Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65515.t1 | KEH41146.1 | 100 | 83 | 0 | 0 | 1 | 83 | 158 | 240 | 1.10E-36 | 162.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65515.t1 | Q8GWQ2 | 78.3 | 83 | 18 | 0 | 1 | 83 | 99 | 181 | 4.4e-31 | 134.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65515.t1 | A0A072VHP8 | 100.0 | 83 | 0 | 0 | 1 | 83 | 158 | 240 | 7.7e-37 | 162.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene65515 | -0.800068 | 1.71E-48 | -1.69E-46 |
| MS.gene049281 | MS.gene65515 | 0.850432 | 1.70E-60 | -1.69E-46 |
| MS.gene049320 | MS.gene65515 | -0.826776 | 2.25E-54 | -1.69E-46 |
| MS.gene049549 | MS.gene65515 | 0.820272 | 7.49E-53 | -1.69E-46 |
| MS.gene049634 | MS.gene65515 | 0.807886 | 4.06E-50 | -1.69E-46 |
| MS.gene050170 | MS.gene65515 | -0.808476 | 3.04E-50 | -1.69E-46 |
| MS.gene050380 | MS.gene65515 | 0.803271 | 3.78E-49 | -1.69E-46 |
| MS.gene050381 | MS.gene65515 | 0.854567 | 1.13E-61 | -1.69E-46 |
| MS.gene050496 | MS.gene65515 | -0.827578 | 1.45E-54 | -1.69E-46 |
| MS.gene050504 | MS.gene65515 | -0.82356 | 1.30E-53 | -1.69E-46 |
| MS.gene050612 | MS.gene65515 | -0.82021 | 7.74E-53 | -1.69E-46 |
| MS.gene050675 | MS.gene65515 | -0.802784 | 4.76E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene65515 | -0.813186 | 2.91E-51 | -1.69E-46 |
| MS.gene050871 | MS.gene65515 | -0.812764 | 3.60E-51 | -1.69E-46 |
| MS.gene050883 | MS.gene65515 | 0.839759 | 1.31E-57 | -1.69E-46 |
| MS.gene051044 | MS.gene65515 | 0.826894 | 2.11E-54 | -1.69E-46 |
| MS.gene05104 | MS.gene65515 | 0.826051 | 3.35E-54 | -1.69E-46 |
| MS.gene051127 | MS.gene65515 | 0.877191 | 7.66E-69 | -1.69E-46 |
| MS.gene051138 | MS.gene65515 | 0.845238 | 4.60E-59 | -1.69E-46 |
| MS.gene051364 | MS.gene65515 | 0.838016 | 3.69E-57 | -1.69E-46 |
| MS.gene051366 | MS.gene65515 | 0.823058 | 1.70E-53 | -1.69E-46 |
| MS.gene051651 | MS.gene65515 | 0.819461 | 1.15E-52 | -1.69E-46 |
| MS.gene051946 | MS.gene65515 | 0.800042 | 1.73E-48 | -1.69E-46 |
| MS.gene052164 | MS.gene65515 | -0.807764 | 4.31E-50 | -1.69E-46 |
| MS.gene052211 | MS.gene65515 | 0.832584 | 8.70E-56 | -1.69E-46 |
| MS.gene05240 | MS.gene65515 | 0.812257 | 4.64E-51 | -1.69E-46 |
| MS.gene052482 | MS.gene65515 | -0.805862 | 1.09E-49 | -1.69E-46 |
| MS.gene052486 | MS.gene65515 | -0.811231 | 7.76E-51 | -1.69E-46 |
| MS.gene053406 | MS.gene65515 | 0.807703 | 4.44E-50 | -1.69E-46 |
| MS.gene053420 | MS.gene65515 | 0.898816 | 3.66E-77 | -1.69E-46 |
| MS.gene053488 | MS.gene65515 | 0.837158 | 6.13E-57 | -1.69E-46 |
| MS.gene053561 | MS.gene65515 | 0.820137 | 8.04E-53 | -1.69E-46 |
| MS.gene053826 | MS.gene65515 | 0.807819 | 4.20E-50 | -1.69E-46 |
| MS.gene053887 | MS.gene65515 | 0.828556 | 8.42E-55 | -1.69E-46 |
| MS.gene054054 | MS.gene65515 | 0.802378 | 5.77E-49 | -1.69E-46 |
| MS.gene054056 | MS.gene65515 | 0.812213 | 4.75E-51 | -1.69E-46 |
| MS.gene054146 | MS.gene65515 | 0.819445 | 1.16E-52 | -1.69E-46 |
| MS.gene054706 | MS.gene65515 | 0.823696 | 1.21E-53 | -1.69E-46 |
| MS.gene054708 | MS.gene65515 | 0.851637 | 7.80E-61 | -1.69E-46 |
| MS.gene05470 | MS.gene65515 | -0.817505 | 3.18E-52 | -1.69E-46 |
| MS.gene054959 | MS.gene65515 | 0.823815 | 1.13E-53 | -1.69E-46 |
| MS.gene055124 | MS.gene65515 | 0.844042 | 9.66E-59 | -1.69E-46 |
| MS.gene055139 | MS.gene65515 | 0.81175 | 5.99E-51 | -1.69E-46 |
| MS.gene055154 | MS.gene65515 | 0.857547 | 1.52E-62 | -1.69E-46 |
| MS.gene055202 | MS.gene65515 | 0.840204 | 1.00E-57 | -1.69E-46 |
| MS.gene055362 | MS.gene65515 | -0.815237 | 1.02E-51 | -1.69E-46 |
| MS.gene055502 | MS.gene65515 | -0.815771 | 7.79E-52 | -1.69E-46 |
| MS.gene055577 | MS.gene65515 | -0.80378 | 2.96E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene65515 | -0.828006 | 1.14E-54 | -1.69E-46 |
| MS.gene055942 | MS.gene65515 | 0.825329 | 4.97E-54 | -1.69E-46 |
| MS.gene056218 | MS.gene65515 | 0.81083 | 9.48E-51 | -1.69E-46 |
| MS.gene056224 | MS.gene65515 | 0.835786 | 1.37E-56 | -1.69E-46 |
| MS.gene056310 | MS.gene65515 | 0.814881 | 1.23E-51 | -1.69E-46 |
| MS.gene056311 | MS.gene65515 | 0.813436 | 2.56E-51 | -1.69E-46 |
| MS.gene056313 | MS.gene65515 | 0.800718 | 1.26E-48 | -1.69E-46 |
| MS.gene056504 | MS.gene65515 | -0.843803 | 1.12E-58 | -1.69E-46 |
| MS.gene056657 | MS.gene65515 | -0.800476 | 1.42E-48 | -1.69E-46 |
| MS.gene056748 | MS.gene65515 | 0.813357 | 2.67E-51 | -1.69E-46 |
| MS.gene056856 | MS.gene65515 | 0.836236 | 1.05E-56 | -1.69E-46 |
| MS.gene057261 | MS.gene65515 | 0.808976 | 2.38E-50 | -1.69E-46 |
| MS.gene057473 | MS.gene65515 | 0.807167 | 5.77E-50 | -1.69E-46 |
| MS.gene057549 | MS.gene65515 | 0.813637 | 2.31E-51 | -1.69E-46 |
| MS.gene057768 | MS.gene65515 | 0.800395 | 1.47E-48 | -1.69E-46 |
| MS.gene058068 | MS.gene65515 | 0.828031 | 1.13E-54 | -1.69E-46 |
| MS.gene058093 | MS.gene65515 | 0.808166 | 3.54E-50 | -1.69E-46 |
| MS.gene058099 | MS.gene65515 | 0.857252 | 1.85E-62 | -1.69E-46 |
| MS.gene058109 | MS.gene65515 | 0.835396 | 1.72E-56 | -1.69E-46 |
| MS.gene058111 | MS.gene65515 | 0.819238 | 1.29E-52 | -1.69E-46 |
| MS.gene058117 | MS.gene65515 | 0.826135 | 3.20E-54 | -1.69E-46 |
| MS.gene058171 | MS.gene65515 | 0.848149 | 7.37E-60 | -1.69E-46 |
| MS.gene058173 | MS.gene65515 | 0.834107 | 3.63E-56 | -1.69E-46 |
| MS.gene058175 | MS.gene65515 | 0.846149 | 2.60E-59 | -1.69E-46 |
| MS.gene058268 | MS.gene65515 | 0.837809 | 4.17E-57 | -1.69E-46 |
| MS.gene058269 | MS.gene65515 | 0.836832 | 7.43E-57 | -1.69E-46 |
| MS.gene058334 | MS.gene65515 | 0.826183 | 3.12E-54 | -1.69E-46 |
| MS.gene05837 | MS.gene65515 | -0.809158 | 2.17E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene65515 | -0.812307 | 4.53E-51 | -1.69E-46 |
| MS.gene058493 | MS.gene65515 | 0.820434 | 6.87E-53 | -1.69E-46 |
| MS.gene058494 | MS.gene65515 | 0.813567 | 2.40E-51 | -1.69E-46 |
| MS.gene058603 | MS.gene65515 | 0.834693 | 2.59E-56 | -1.69E-46 |
| MS.gene058628 | MS.gene65515 | 0.816971 | 4.20E-52 | -1.69E-46 |
| MS.gene058629 | MS.gene65515 | 0.814407 | 1.57E-51 | -1.69E-46 |
| MS.gene058630 | MS.gene65515 | 0.818502 | 1.90E-52 | -1.69E-46 |
| MS.gene059022 | MS.gene65515 | -0.831302 | 1.80E-55 | -1.69E-46 |
| MS.gene059037 | MS.gene65515 | 0.80503 | 1.62E-49 | -1.69E-46 |
| MS.gene059045 | MS.gene65515 | 0.821413 | 4.09E-53 | -1.69E-46 |
| MS.gene059223 | MS.gene65515 | 0.81207 | 5.10E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene65515 | -0.809902 | 1.50E-50 | -1.69E-46 |
| MS.gene059694 | MS.gene65515 | 0.810857 | 9.36E-51 | -1.69E-46 |
| MS.gene059697 | MS.gene65515 | 0.811667 | 6.24E-51 | -1.69E-46 |
| MS.gene059865 | MS.gene65515 | -0.840916 | 6.52E-58 | -1.69E-46 |
| MS.gene059916 | MS.gene65515 | 0.82892 | 6.87E-55 | -1.69E-46 |
| MS.gene059953 | MS.gene65515 | 0.819822 | 9.49E-53 | -1.69E-46 |
| MS.gene059955 | MS.gene65515 | 0.849349 | 3.42E-60 | -1.69E-46 |
| MS.gene060593 | MS.gene65515 | 0.84472 | 6.35E-59 | -1.69E-46 |
| MS.gene060595 | MS.gene65515 | 0.825992 | 3.46E-54 | -1.69E-46 |
| MS.gene060778 | MS.gene65515 | -0.814727 | 1.33E-51 | -1.69E-46 |
| MS.gene06077 | MS.gene65515 | -0.841546 | 4.45E-58 | -1.69E-46 |
| MS.gene060982 | MS.gene65515 | -0.804511 | 2.09E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65515.t1 | MTR_1g044155 | 100.000 | 83 | 0 | 0 | 1 | 83 | 158 | 240 | 2.73e-50 | 161 |
| MS.gene65515.t1 | MTR_1g073860 | 91.250 | 80 | 7 | 0 | 1 | 80 | 126 | 205 | 1.87e-45 | 148 |
| MS.gene65515.t1 | MTR_3g068035 | 78.750 | 80 | 17 | 0 | 1 | 80 | 111 | 190 | 2.04e-38 | 129 |
| MS.gene65515.t1 | MTR_1g079810 | 71.250 | 80 | 23 | 0 | 1 | 80 | 106 | 185 | 6.55e-38 | 128 |
| MS.gene65515.t1 | MTR_7g080980 | 74.684 | 79 | 20 | 0 | 1 | 79 | 89 | 167 | 6.68e-37 | 124 |
| MS.gene65515.t1 | MTR_5g091630 | 70.886 | 79 | 23 | 0 | 1 | 79 | 79 | 157 | 1.20e-36 | 124 |
| MS.gene65515.t1 | MTR_4g098450 | 71.250 | 80 | 23 | 0 | 1 | 80 | 108 | 187 | 6.37e-36 | 122 |
| MS.gene65515.t1 | MTR_5g011520 | 75.000 | 80 | 20 | 0 | 1 | 80 | 132 | 211 | 1.34e-32 | 115 |
| MS.gene65515.t1 | MTR_8g036060 | 75.000 | 80 | 20 | 0 | 1 | 80 | 118 | 197 | 9.14e-32 | 112 |
| MS.gene65515.t1 | MTR_5g080580 | 65.000 | 80 | 28 | 0 | 1 | 80 | 121 | 200 | 6.74e-31 | 110 |
| MS.gene65515.t1 | MTR_3g100470 | 50.575 | 87 | 39 | 2 | 1 | 83 | 106 | 192 | 8.97e-24 | 91.3 |
| MS.gene65515.t1 | MTR_5g066020 | 46.512 | 86 | 43 | 2 | 1 | 83 | 85 | 170 | 1.28e-21 | 84.7 |
| MS.gene65515.t1 | MTR_7g116320 | 39.241 | 79 | 48 | 0 | 3 | 81 | 150 | 228 | 3.83e-16 | 70.9 |
| MS.gene65515.t1 | MTR_7g074935 | 44.615 | 65 | 36 | 0 | 1 | 65 | 132 | 196 | 1.09e-15 | 70.1 |
| MS.gene65515.t1 | MTR_7g116320 | 39.744 | 78 | 47 | 0 | 3 | 80 | 150 | 227 | 1.40e-15 | 69.7 |
| MS.gene65515.t1 | MTR_8g036160 | 40.476 | 84 | 46 | 1 | 1 | 80 | 147 | 230 | 4.67e-15 | 68.2 |
| MS.gene65515.t1 | MTR_5g010330 | 49.123 | 57 | 29 | 0 | 8 | 64 | 146 | 202 | 1.91e-13 | 63.5 |
| MS.gene65515.t1 | MTR_4g101990 | 42.424 | 66 | 38 | 0 | 1 | 66 | 149 | 214 | 2.85e-13 | 63.2 |
| MS.gene65515.t1 | MTR_1g076710 | 39.286 | 84 | 47 | 2 | 1 | 81 | 139 | 221 | 3.92e-13 | 62.8 |
| MS.gene65515.t1 | MTR_8g098390 | 45.763 | 59 | 32 | 0 | 6 | 64 | 176 | 234 | 2.99e-12 | 60.5 |
| MS.gene65515.t1 | MTR_1g079780 | 37.975 | 79 | 48 | 1 | 3 | 80 | 116 | 194 | 3.01e-11 | 57.8 |
| MS.gene65515.t1 | MTR_6g061670 | 38.462 | 65 | 40 | 0 | 1 | 65 | 130 | 194 | 5.95e-11 | 56.6 |
| MS.gene65515.t1 | MTR_6g061670 | 38.462 | 65 | 40 | 0 | 1 | 65 | 135 | 199 | 6.32e-11 | 56.6 |
| MS.gene65515.t1 | MTR_1g061530 | 40.323 | 62 | 36 | 1 | 3 | 63 | 148 | 209 | 6.63e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65515.t1 | AT4G14465 | 78.750 | 80 | 17 | 0 | 1 | 80 | 99 | 178 | 2.51e-40 | 134 |
| MS.gene65515.t1 | AT2G42940 | 64.198 | 81 | 29 | 0 | 1 | 81 | 85 | 165 | 4.70e-34 | 117 |
| MS.gene65515.t1 | AT3G04570 | 64.773 | 88 | 23 | 1 | 1 | 80 | 112 | 199 | 1.96e-33 | 117 |
| MS.gene65515.t1 | AT4G17800 | 67.500 | 80 | 26 | 0 | 1 | 80 | 114 | 193 | 3.18e-33 | 115 |
| MS.gene65515.t1 | AT2G35270 | 66.667 | 81 | 27 | 0 | 1 | 81 | 110 | 190 | 3.97e-33 | 115 |
| MS.gene65515.t1 | AT3G60870 | 69.880 | 83 | 23 | 1 | 1 | 83 | 91 | 171 | 3.84e-29 | 104 |
| MS.gene65515.t1 | AT4G22810 | 69.880 | 83 | 22 | 1 | 1 | 80 | 137 | 219 | 1.67e-28 | 104 |
| MS.gene65515.t1 | AT4G12050 | 69.136 | 81 | 24 | 1 | 1 | 80 | 150 | 230 | 1.92e-28 | 104 |
| MS.gene65515.t1 | AT2G45430 | 70.238 | 84 | 21 | 2 | 1 | 80 | 121 | 204 | 9.44e-28 | 102 |
| MS.gene65515.t1 | AT3G55560 | 66.250 | 80 | 26 | 1 | 1 | 79 | 120 | 199 | 4.37e-27 | 100 |
| MS.gene65515.t1 | AT5G49700 | 55.556 | 90 | 33 | 2 | 1 | 83 | 88 | 177 | 8.75e-25 | 93.6 |
| MS.gene65515.t1 | AT1G76500 | 61.957 | 92 | 26 | 1 | 1 | 83 | 104 | 195 | 2.12e-23 | 90.5 |
| MS.gene65515.t1 | AT4G35390 | 67.073 | 82 | 25 | 1 | 1 | 80 | 95 | 176 | 6.17e-23 | 89.4 |
| MS.gene65515.t1 | AT1G20900 | 61.798 | 89 | 25 | 1 | 1 | 80 | 118 | 206 | 3.09e-19 | 79.3 |
| MS.gene65515.t1 | AT1G14490 | 56.923 | 65 | 26 | 1 | 1 | 63 | 35 | 99 | 3.96e-19 | 77.4 |
| MS.gene65515.t1 | AT1G14490 | 56.923 | 65 | 26 | 1 | 1 | 63 | 54 | 118 | 5.14e-19 | 77.8 |
| MS.gene65515.t1 | AT4G12080 | 42.424 | 66 | 38 | 0 | 1 | 66 | 175 | 240 | 1.69e-14 | 67.0 |
| MS.gene65515.t1 | AT5G62260 | 41.463 | 82 | 45 | 1 | 3 | 81 | 167 | 248 | 1.78e-14 | 66.2 |
| MS.gene65515.t1 | AT5G62260 | 41.463 | 82 | 45 | 1 | 3 | 81 | 167 | 248 | 2.06e-14 | 66.6 |
| MS.gene65515.t1 | AT5G62260 | 41.463 | 82 | 45 | 1 | 3 | 81 | 167 | 248 | 2.06e-14 | 66.6 |
| MS.gene65515.t1 | AT5G51590 | 39.535 | 86 | 45 | 2 | 1 | 81 | 183 | 266 | 2.11e-14 | 66.6 |
| MS.gene65515.t1 | AT1G63470 | 43.750 | 64 | 36 | 0 | 1 | 64 | 182 | 245 | 2.46e-14 | 66.6 |
| MS.gene65515.t1 | AT4G25320 | 41.463 | 82 | 45 | 1 | 3 | 81 | 173 | 254 | 3.68e-14 | 65.9 |
| MS.gene65515.t1 | AT4G00200 | 40.625 | 64 | 38 | 0 | 1 | 64 | 128 | 191 | 1.05e-13 | 64.3 |
| MS.gene65515.t1 | AT4G00200 | 40.625 | 64 | 38 | 0 | 1 | 64 | 128 | 191 | 1.05e-13 | 64.3 |
| MS.gene65515.t1 | AT4G22770 | 41.538 | 65 | 38 | 0 | 1 | 65 | 155 | 219 | 6.32e-13 | 62.4 |
| MS.gene65515.t1 | AT4G22770 | 41.538 | 65 | 38 | 0 | 1 | 65 | 155 | 219 | 6.32e-13 | 62.4 |
| MS.gene65515.t1 | AT2G33620 | 46.032 | 63 | 34 | 0 | 1 | 63 | 168 | 230 | 1.01e-12 | 62.0 |
| MS.gene65515.t1 | AT2G33620 | 46.032 | 63 | 34 | 0 | 1 | 63 | 168 | 230 | 1.01e-12 | 62.0 |
| MS.gene65515.t1 | AT2G33620 | 46.032 | 63 | 34 | 0 | 1 | 63 | 168 | 230 | 1.01e-12 | 62.0 |
| MS.gene65515.t1 | AT2G33620 | 46.032 | 63 | 34 | 0 | 1 | 63 | 168 | 230 | 1.01e-12 | 62.0 |
| MS.gene65515.t1 | AT3G04590 | 35.000 | 80 | 52 | 0 | 1 | 80 | 176 | 255 | 1.99e-12 | 60.8 |
| MS.gene65515.t1 | AT3G04590 | 35.000 | 80 | 52 | 0 | 1 | 80 | 176 | 255 | 2.22e-12 | 60.8 |
| MS.gene65515.t1 | AT4G17950 | 42.424 | 66 | 36 | 1 | 1 | 64 | 226 | 291 | 3.37e-12 | 60.5 |
| MS.gene65515.t1 | AT5G46640 | 40.625 | 64 | 38 | 0 | 1 | 64 | 185 | 248 | 7.95e-12 | 59.3 |
Find 28 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGTCGCCTTCTTGCAAAC+TGG | 0.280850 | 1.4:+28584473 | None:intergenic |
| CTGGTGCTGCTGGTTGTCTA+AGG | 0.295952 | 1.4:+28584404 | None:intergenic |
| CCTGCTCCTCCAGGCGCAAC+CGG | 0.373469 | 1.4:-28584316 | MS.gene65515:CDS |
| GGAGCTGTTGTAGCACTTCA+TGG | 0.401949 | 1.4:-28584382 | MS.gene65515:CDS |
| TTTGATATTCTATCGTTAAC+CGG | 0.404149 | 1.4:-28584355 | MS.gene65515:CDS |
| CTACCGGGACCTGCTCCTCC+AGG | 0.422866 | 1.4:-28584325 | MS.gene65515:CDS |
| ACAACAGCTCCTGGTGCTGC+TGG | 0.430712 | 1.4:+28584394 | None:intergenic |
| ACTGTGAGTCCGGTTGCGCC+TGG | 0.442371 | 1.4:+28584307 | None:intergenic |
| CCGGTTGCGCCTGGAGGAGC+AGG | 0.444168 | 1.4:+28584316 | None:intergenic |
| TTGTCGCCTTCTTGCAAACT+GGG | 0.462433 | 1.4:+28584474 | None:intergenic |
| GGCTAAGTACACTGTGAGTC+CGG | 0.495462 | 1.4:+28584297 | None:intergenic |
| GTTTGTGTTCTAAGCGGAAG+TGG | 0.522918 | 1.4:-28584445 | MS.gene65515:CDS |
| GCGCCTGGAGGAGCAGGTCC+CGG | 0.523911 | 1.4:+28584322 | None:intergenic |
| AACAACTTGACCTTGTCCAC+CGG | 0.525030 | 1.4:+28584276 | None:intergenic |
| TGAAGTGCTACAACAGCTCC+TGG | 0.542969 | 1.4:+28584385 | None:intergenic |
| CTGTTGTAGCACTTCATGGA+AGG | 0.544498 | 1.4:-28584378 | MS.gene65515:CDS |
| TGGTGCTGCTGGTTGTCTAA+GGG | 0.547569 | 1.4:+28584405 | None:intergenic |
| AGGTCCCGGTAGAAAAGCTC+CGG | 0.559148 | 1.4:+28584336 | None:intergenic |
| GTGAGTCCGGTTGCGCCTGG+AGG | 0.561910 | 1.4:+28584310 | None:intergenic |
| GTGTACTTAGCCGGTGGACA+AGG | 0.563201 | 1.4:-28584286 | None:intergenic |
| CTTAGACAACCAGCAGCACC+AGG | 0.566004 | 1.4:-28584403 | MS.gene65515:CDS |
| CATGTGATGGAAGTATCCTC+TGG | 0.566447 | 1.4:-28584520 | None:intergenic |
| TTCAGCTACATCTGCTCCAG+AGG | 0.579175 | 1.4:+28584504 | None:intergenic |
| GTGTAGCCCAGTTTGCAAGA+AGG | 0.599811 | 1.4:-28584480 | MS.gene65515:CDS |
| GGACTCACAGTGTACTTAGC+CGG | 0.612698 | 1.4:-28584295 | MS.gene65515:CDS |
| CGCGGTGTTTGTGTTCTAAG+CGG | 0.639393 | 1.4:-28584451 | MS.gene65515:CDS |
| CTCACAGTGTACTTAGCCGG+TGG | 0.652416 | 1.4:-28584292 | MS.gene65515:CDS |
| TTTGCAAGAAGGCGACAACG+CGG | 0.760437 | 1.4:-28584469 | MS.gene65515:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTGATATTCTATCGTTAAC+CGG | - | chr1.4:28584446-28584465 | MS.gene65515:CDS | 25.0% |
| CTGTTGTAGCACTTCATGGA+AGG | - | chr1.4:28584423-28584442 | MS.gene65515:CDS | 45.0% | |
| TTGTCGCCTTCTTGCAAACT+GGG | + | chr1.4:28584330-28584349 | None:intergenic | 45.0% | |
| ! | GTTAACCGGAGCTTTTCTAC+CGG | - | chr1.4:28584460-28584479 | MS.gene65515:CDS | 45.0% |
| ! | GTTTGTGTTCTAAGCGGAAG+TGG | - | chr1.4:28584356-28584375 | MS.gene65515:CDS | 45.0% |
| ! | TTAACCGGAGCTTTTCTACC+GGG | - | chr1.4:28584461-28584480 | MS.gene65515:CDS | 45.0% |
| GGACTCACAGTGTACTTAGC+CGG | - | chr1.4:28584506-28584525 | MS.gene65515:CDS | 50.0% | |
| GGAGCTGTTGTAGCACTTCA+TGG | - | chr1.4:28584419-28584438 | MS.gene65515:CDS | 50.0% | |
| GGCTAAGTACACTGTGAGTC+CGG | + | chr1.4:28584507-28584526 | None:intergenic | 50.0% | |
| GTGTAGCCCAGTTTGCAAGA+AGG | - | chr1.4:28584321-28584340 | MS.gene65515:CDS | 50.0% | |
| GTTGTCGCCTTCTTGCAAAC+TGG | + | chr1.4:28584331-28584350 | None:intergenic | 50.0% | |
| TTCAGCTACATCTGCTCCAG+AGG | + | chr1.4:28584300-28584319 | None:intergenic | 50.0% | |
| TTTGCAAGAAGGCGACAACG+CGG | - | chr1.4:28584332-28584351 | MS.gene65515:CDS | 50.0% | |
| ! | CGCGGTGTTTGTGTTCTAAG+CGG | - | chr1.4:28584350-28584369 | MS.gene65515:CDS | 50.0% |
| ! | TGAAGTGCTACAACAGCTCC+TGG | + | chr1.4:28584419-28584438 | None:intergenic | 50.0% |
| !! | TGGTGCTGCTGGTTGTCTAA+GGG | + | chr1.4:28584399-28584418 | None:intergenic | 50.0% |
| AGGTCCCGGTAGAAAAGCTC+CGG | + | chr1.4:28584468-28584487 | None:intergenic | 55.0% | |
| CTCACAGTGTACTTAGCCGG+TGG | - | chr1.4:28584509-28584528 | MS.gene65515:CDS | 55.0% | |
| CTTAGACAACCAGCAGCACC+AGG | - | chr1.4:28584398-28584417 | MS.gene65515:CDS | 55.0% | |
| !! | CTGGTGCTGCTGGTTGTCTA+AGG | + | chr1.4:28584400-28584419 | None:intergenic | 55.0% |
| !! | ACAACAGCTCCTGGTGCTGC+TGG | + | chr1.4:28584410-28584429 | None:intergenic | 60.0% |
| ACTGTGAGTCCGGTTGCGCC+TGG | + | chr1.4:28584497-28584516 | None:intergenic | 65.0% | |
| CCTGCTCCTCCAGGCGCAAC+CGG | - | chr1.4:28584485-28584504 | MS.gene65515:CDS | 70.0% | |
| CTACCGGGACCTGCTCCTCC+AGG | - | chr1.4:28584476-28584495 | MS.gene65515:CDS | 70.0% | |
| GTGAGTCCGGTTGCGCCTGG+AGG | + | chr1.4:28584494-28584513 | None:intergenic | 70.0% | |
| CCGGTTGCGCCTGGAGGAGC+AGG | + | chr1.4:28584488-28584507 | None:intergenic | 75.0% | |
| GCGCCTGGAGGAGCAGGTCC+CGG | + | chr1.4:28584482-28584501 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 28584287 | 28584536 | 28584287 | ID=MS.gene65515 |
| chr1.4 | mRNA | 28584287 | 28584536 | 28584287 | ID=MS.gene65515.t1;Parent=MS.gene65515 |
| chr1.4 | exon | 28584287 | 28584536 | 28584287 | ID=MS.gene65515.t1.exon1;Parent=MS.gene65515.t1 |
| chr1.4 | CDS | 28584287 | 28584536 | 28584287 | ID=cds.MS.gene65515.t1;Parent=MS.gene65515.t1 |
| Gene Sequence |
| Protein sequence |
