AlfalfaGEDB | MS.gene70685

M. sativa cultivar XinJiangDaYe / MS.gene70685

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70685.t1	AES89321.1	89.5	86	9	0	1	86	1	86	3.20E-36	160.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70685.t1	P33080	64.4	90	28	1	1	86	1	90	8.0e-28	124.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70685.t1	G7JL46	89.5	86	9	0	1	86	1	86	2.3e-36	160.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene003615	MS.gene70685	PPI
MS.gene70685	MS.gene79886	PPI
MS.gene70685	MS.gene50765	PPI
MS.gene062973	MS.gene70685	PPI
MS.gene003602	MS.gene70685	PPI
MS.gene23372	MS.gene70685	PPI
MS.gene047517	MS.gene70685	PPI
MS.gene003627	MS.gene70685	PPI
MS.gene003577	MS.gene70685	PPI
MS.gene77524	MS.gene70685	PPI
MS.gene062984	MS.gene70685	PPI
MS.gene057801	MS.gene70685	PPI
MS.gene014223	MS.gene70685	PPI
MS.gene40327	MS.gene70685	PPI
MS.gene70685	MS.gene79891	PPI
MS.gene70685	MS.gene045367	PPI
MS.gene045368	MS.gene70685	PPI
MS.gene045362	MS.gene70685	PPI
MS.gene70685	MS.gene014669	PPI
MS.gene70685	MS.gene057808	PPI
MS.gene70685	MS.gene002380	PPI
MS.gene003587	MS.gene70685	PPI
MS.gene003590	MS.gene70685	PPI
MS.gene062943	MS.gene70685	PPI
MS.gene063112	MS.gene70685	PPI
MS.gene70685	MS.gene68163	PPI
MS.gene003608	MS.gene70685	PPI
MS.gene70685	MS.gene75315	PPI
MS.gene70685	MS.gene68164	PPI
MS.gene77526	MS.gene70685	PPI
MS.gene045360	MS.gene70685	PPI
MS.gene70685	MS.gene014222	PPI
MS.gene70685	MS.gene79888	PPI
MS.gene057818	MS.gene70685	PPI
MS.gene70685	MS.gene75678	PPI
MS.gene50687	MS.gene70685	PPI
MS.gene75677	MS.gene70685	PPI
MS.gene003625	MS.gene70685	PPI
MS.gene057795	MS.gene70685	PPI
MS.gene70685	MS.gene79885	PPI
MS.gene68165	MS.gene70685	PPI
MS.gene045361	MS.gene70685	PPI
MS.gene50675	MS.gene70685	PPI
MS.gene003591	MS.gene70685	PPI
MS.gene70685	MS.gene50764	PPI
MS.gene062979	MS.gene70685	PPI
MS.gene40325	MS.gene70685	PPI
MS.gene01358	MS.gene70685	PPI
MS.gene75675	MS.gene70685	PPI
MS.gene003583	MS.gene70685	PPI
MS.gene40326	MS.gene70685	PPI
MS.gene062952	MS.gene70685	PPI
MS.gene77527	MS.gene70685	PPI
MS.gene70685	MS.gene014670	PPI
MS.gene031199	MS.gene70685	PPI
MS.gene50676	MS.gene70685	PPI
MS.gene003614	MS.gene70685	PPI
MS.gene057797	MS.gene70685	PPI
MS.gene23370	MS.gene70685	PPI
MS.gene50766	MS.gene70685	PPI
MS.gene77529	MS.gene70685	PPI
MS.gene014667	MS.gene70685	PPI
MS.gene003594	MS.gene70685	PPI
MS.gene70685	MS.gene01764	PPI
MS.gene003596	MS.gene70685	PPI
MS.gene50665	MS.gene70685	PPI
MS.gene79889	MS.gene70685	PPI
MS.gene50677	MS.gene70685	PPI
MS.gene70685	MS.gene75679	PPI
MS.gene68759	MS.gene70685	PPI
MS.gene003586	MS.gene70685	PPI
MS.gene062988	MS.gene70685	PPI
MS.gene75673	MS.gene70685	PPI
MS.gene77528	MS.gene70685	PPI
MS.gene50763	MS.gene70685	PPI
MS.gene031198	MS.gene70685	PPI
MS.gene031200	MS.gene70685	PPI
MS.gene045365	MS.gene70685	PPI
MS.gene70685	MS.gene014671	PPI
MS.gene68760	MS.gene70685	PPI
MS.gene045364	MS.gene70685	PPI
MS.gene50659	MS.gene70685	PPI
MS.gene70685	MS.gene75672	PPI
MS.gene75316	MS.gene70685	PPI
MS.gene70685	MS.gene50654	PPI
MS.gene057790	MS.gene70685	PPI
MS.gene70685	MS.gene062974	PPI
MS.gene003624	MS.gene70685	PPI
MS.gene70685	MS.gene062951	PPI
MS.gene23371	MS.gene70685	PPI
MS.gene031201	MS.gene70685	PPI
MS.gene70685	MS.gene68162	PPI
MS.gene75314	MS.gene70685	PPI
MS.gene70685	MS.gene045363	PPI
MS.gene68761	MS.gene70685	PPI
MS.gene70685	MS.gene79887	PPI
MS.gene75676	MS.gene70685	PPI
MS.gene70685	MS.gene50652	PPI
MS.gene77523	MS.gene70685	PPI
MS.gene057778	MS.gene70685	PPI
MS.gene045366	MS.gene70685	PPI
MS.gene062963	MS.gene70685	PPI
MS.gene70685	MS.gene50684	PPI
MS.gene75674	MS.gene70685	PPI
MS.gene70685	MS.gene014668	PPI
MS.gene79890	MS.gene70685	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70685.t1	MTR_4g072630	88.372	86	10	0	1	86	1	86	1.76e-53	161
MS.gene70685.t1	MTR_4g072465	84.884	86	13	0	1	86	1	86	6.75e-51	154
MS.gene70685.t1	MTR_4g072740	86.047	86	11	1	1	86	1	85	9.41e-51	154
MS.gene70685.t1	MTR_4g072490	87.209	86	10	1	1	86	1	85	1.38e-50	154
MS.gene70685.t1	MTR_4g072470	82.558	86	14	1	1	86	1	85	1.86e-46	143
MS.gene70685.t1	MTR_4g072580	80.233	86	16	1	1	86	1	85	5.36e-46	142
MS.gene70685.t1	MTR_4g072370	78.409	88	17	1	1	86	1	88	5.92e-46	142
MS.gene70685.t1	MTR_4g072510	89.535	86	9	0	1	86	1	86	1.03e-45	141
MS.gene70685.t1	MTR_4g072670	87.209	86	11	0	1	86	1	86	1.88e-45	140
MS.gene70685.t1	MTR_4g072830	78.161	87	17	1	1	85	1	87	6.13e-45	139
MS.gene70685.t1	MTR_4g072540	87.209	86	11	0	1	86	1	86	3.22e-44	137
MS.gene70685.t1	MTR_4g072720	87.209	86	11	0	1	86	1	86	3.52e-44	137
MS.gene70685.t1	MTR_4g072600	76.744	86	19	1	1	86	1	85	3.63e-43	135
MS.gene70685.t1	MTR_4g072610	77.907	86	17	1	1	86	1	84	3.65e-43	135
MS.gene70685.t1	MTR_4g072650	76.744	86	18	1	1	86	1	84	1.89e-42	133
MS.gene70685.t1	MTR_3g084150	70.000	90	23	1	1	86	1	90	8.06e-42	132
MS.gene70685.t1	MTR_3g084220	68.889	90	24	1	1	86	1	90	9.40e-42	132
MS.gene70685.t1	MTR_3g084170	70.000	90	23	1	1	86	1	90	2.79e-41	130
MS.gene70685.t1	MTR_4g072330	71.910	89	21	2	1	86	1	88	3.16e-41	130
MS.gene70685.t1	MTR_5g008350	81.333	75	13	1	1	75	1	74	4.19e-41	129
MS.gene70685.t1	MTR_8g461410	68.889	90	24	1	1	86	1	90	4.77e-41	130
MS.gene70685.t1	MTR_3g084180	68.889	90	24	1	1	86	1	90	5.50e-41	130
MS.gene70685.t1	MTR_3g110038	68.889	90	24	1	1	86	1	90	2.39e-40	128
MS.gene70685.t1	MTR_4g072870	68.478	92	22	2	1	86	1	91	2.92e-40	128
MS.gene70685.t1	MTR_3g084240	68.889	90	24	1	1	86	1	90	3.71e-40	127
MS.gene70685.t1	MTR_3g110032	65.934	91	26	1	1	86	1	91	3.00e-39	125
MS.gene70685.t1	MTR_4g072590	77.907	86	18	1	1	86	1	85	5.78e-39	124
MS.gene70685.t1	MTR_4g072310	67.033	91	25	1	1	86	1	91	6.33e-39	124
MS.gene70685.t1	MTR_4g072240	64.835	91	27	1	1	86	1	91	1.42e-38	124
MS.gene70685.t1	MTR_4g050700	67.033	91	25	1	1	86	1	91	1.47e-38	124
MS.gene70685.t1	MTR_2g012410	67.033	91	25	1	1	86	1	91	1.47e-38	124
MS.gene70685.t1	MTR_4g072880	65.934	91	26	1	1	86	1	91	3.39e-38	122
MS.gene70685.t1	MTR_4g072690	75.581	86	20	1	1	86	1	85	3.90e-38	122
MS.gene70685.t1	MTR_3g110042	70.588	85	21	1	1	81	1	85	4.24e-38	122
MS.gene70685.t1	MTR_4g072480	64.444	90	28	1	1	86	1	90	5.96e-38	122
MS.gene70685.t1	MTR_4g072380	72.414	87	22	2	1	86	1	86	6.00e-38	122
MS.gene70685.t1	MTR_4g072450	76.744	86	19	1	1	86	1	85	8.41e-38	121
MS.gene70685.t1	MTR_3g084260	64.835	91	27	1	1	86	1	91	1.43e-37	121
MS.gene70685.t1	MTR_4g072300	62.637	91	29	1	1	86	1	91	2.06e-37	120
MS.gene70685.t1	MTR_4g072220	62.637	91	29	1	1	86	1	91	3.87e-37	120
MS.gene70685.t1	MTR_4g072890	62.637	91	29	1	1	86	1	91	4.12e-37	120
MS.gene70685.t1	MTR_4g072270	64.835	91	27	1	1	86	1	91	4.14e-37	120
MS.gene70685.t1	MTR_4g072250	69.231	91	22	2	1	86	1	90	4.68e-37	120
MS.gene70685.t1	MTR_3g084250	65.556	90	27	1	1	86	1	90	9.98e-37	119
MS.gene70685.t1	MTR_4g072820	71.264	87	22	2	1	86	1	85	1.43e-36	118
MS.gene70685.t1	MTR_4g072570	64.444	90	28	1	1	86	1	90	1.45e-36	119
MS.gene70685.t1	MTR_4g072620	61.111	90	31	1	1	86	1	90	1.63e-36	118
MS.gene70685.t1	MTR_4g072860	69.565	92	21	2	1	86	1	91	1.78e-36	118
MS.gene70685.t1	MTR_4g072680	63.333	90	29	1	1	86	1	90	1.97e-36	118
MS.gene70685.t1	MTR_4g072260	61.538	91	30	1	1	86	1	91	2.67e-36	118
MS.gene70685.t1	MTR_4g072850	66.304	92	25	1	1	86	1	92	4.91e-36	117
MS.gene70685.t1	MTR_4g072500	64.444	90	28	1	1	86	1	90	2.80e-35	115
MS.gene70685.t1	MTR_4g072530	63.333	90	29	1	1	86	1	90	3.36e-35	115
MS.gene70685.t1	MTR_4g072730	60.000	90	32	1	1	86	1	90	3.72e-35	115
MS.gene70685.t1	MTR_4g072460	61.111	90	31	1	1	86	1	90	4.84e-35	115
MS.gene70685.t1	MTR_3g117630	71.233	73	21	0	14	86	18	90	9.45e-35	114
MS.gene70685.t1	MTR_3g084210	64.444	90	28	1	1	86	1	90	1.62e-34	113
MS.gene70685.t1	MTR_4g072640	63.736	91	27	2	1	86	1	90	2.59e-34	113
MS.gene70685.t1	MTR_3g084190	66.667	90	26	1	1	86	1	90	2.92e-34	112
MS.gene70685.t1	MTR_8g461420	66.667	90	26	1	1	86	1	90	4.06e-34	112
MS.gene70685.t1	MTR_4g072230	63.736	91	26	2	1	86	1	89	8.88e-34	111
MS.gene70685.t1	MTR_3g084230	65.556	90	27	1	1	86	1	90	1.11e-33	111
MS.gene70685.t1	MTR_4g072660	60.465	86	33	1	1	86	1	85	1.34e-33	111
MS.gene70685.t1	MTR_4g072940	64.444	90	26	1	3	86	33	122	4.92e-33	110
MS.gene70685.t1	MTR_3g084160	65.556	90	27	1	1	86	1	90	5.40e-33	109
MS.gene70685.t1	MTR_5g008370	63.333	90	29	1	1	86	1	90	2.56e-32	109
MS.gene70685.t1	MTR_5g008360	64.103	78	28	0	9	86	4	81	3.14e-32	107
MS.gene70685.t1	MTR_3g110045	65.909	88	26	1	3	86	62	149	3.64e-32	109
MS.gene70685.t1	MTR_3g084200	68.056	72	19	1	1	68	1	72	4.44e-31	105
MS.gene70685.t1	MTR_4g072980	55.435	92	34	2	1	85	1	92	1.08e-29	101
MS.gene70685.t1	MTR_4g072560	51.546	97	35	3	1	85	1	97	1.41e-28	99.0
MS.gene70685.t1	MTR_4g072770	58.140	86	30	1	1	86	1	80	2.28e-28	97.8
MS.gene70685.t1	MTR_4g072910	61.039	77	29	1	10	86	25	100	1.52e-27	96.3
MS.gene70685.t1	MTR_4g072550	63.380	71	26	0	16	86	31	101	2.46e-27	95.9
MS.gene70685.t1	MTR_3g117640	61.765	68	25	1	20	86	33	100	7.64e-27	94.7
MS.gene70685.t1	MTR_4g072750	63.514	74	21	2	1	68	1	74	1.86e-24	87.8
MS.gene70685.t1	MTR_4g072280	56.000	75	28	1	1	70	1	75	3.94e-24	87.0
MS.gene70685.t1	MTR_4g072290	54.651	86	30	2	1	86	1	77	1.18e-23	85.9
MS.gene70685.t1	MTR_3g110035	59.375	64	26	0	23	86	73	136	7.08e-23	85.5
MS.gene70685.t1	MTR_3g117610	57.812	64	26	1	19	82	43	105	3.75e-22	82.8
MS.gene70685.t1	MTR_4g072190	50.000	72	35	1	11	82	26	96	4.63e-21	79.7
MS.gene70685.t1	MTR_3g113310	52.239	67	31	1	19	85	40	105	1.07e-20	79.0
MS.gene70685.t1	MTR_6g077850	49.254	67	34	0	20	86	33	99	1.59e-19	75.9
MS.gene70685.t1	MTR_4g072930	46.914	81	38	2	11	86	30	110	5.67e-19	74.7
MS.gene70685.t1	MTR_5g021820	45.679	81	41	2	4	82	21	100	1.53e-18	73.6
MS.gene70685.t1	MTR_8g461400	45.556	90	19	2	1	86	1	64	1.59e-17	69.7
MS.gene70685.t1	MTR_8g076040	54.237	59	26	1	20	78	40	97	2.91e-17	70.5
MS.gene70685.t1	MTR_4g072840	49.425	87	16	3	1	86	1	60	7.35e-17	68.2
MS.gene70685.t1	MTR_1g061570	45.783	83	42	2	5	85	1	82	4.17e-16	67.8
MS.gene70685.t1	MTR_2g043960	54.237	59	26	1	20	78	75	132	5.95e-16	68.6
MS.gene70685.t1	MTR_4g124750	52.542	59	27	1	20	78	68	125	1.48e-15	67.4
MS.gene70685.t1	MTR_1g094750	47.692	65	33	1	20	84	49	112	8.82e-15	64.3
MS.gene70685.t1	MTR_1g009150	49.275	69	34	1	13	81	63	130	1.08e-14	65.5
MS.gene70685.t1	MTR_4g005310	48.387	62	31	1	21	81	62	123	2.24e-14	64.3
MS.gene70685.t1	MTR_1g063950	45.161	62	33	1	20	81	47	107	5.14e-14	62.4
MS.gene70685.t1	MTR_0388s0020	46.269	67	32	2	11	74	65	130	5.91e-14	63.2
MS.gene70685.t1	MTR_3g105900	77.778	36	8	0	20	55	1171	1206	5.99e-14	65.5
MS.gene70685.t1	MTR_7g101740	48.387	62	31	1	20	81	39	99	1.16e-13	61.2
MS.gene70685.t1	MTR_4g072160	44.000	75	40	2	8	81	23	96	2.30e-13	61.2
MS.gene70685.t1	MTR_4g005320	47.541	61	31	1	21	81	70	129	6.32e-13	60.5
MS.gene70685.t1	MTR_4g025830	48.333	60	27	2	18	74	76	134	7.87e-13	60.5
MS.gene70685.t1	MTR_7g118010	47.619	63	30	2	21	81	23	84	9.48e-13	58.9
MS.gene70685.t1	MTR_1g024085	45.312	64	34	1	20	83	36	98	1.73e-12	59.3
MS.gene70685.t1	MTR_3g092220	43.077	65	34	2	12	74	24	87	2.59e-12	57.8
MS.gene70685.t1	MTR_3g109160	49.180	61	30	1	21	81	69	128	7.26e-12	58.2
MS.gene70685.t1	MTR_8g461210	40.506	79	42	2	4	78	14	91	8.68e-12	56.6
MS.gene70685.t1	MTR_5g008380	78.125	32	7	0	55	86	246	277	1.12e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70685.t1	AT4G38840	67.647	68	21	1	20	86	32	99	2.30e-30	103
MS.gene70685.t1	AT4G34800	57.143	84	33	1	1	81	1	84	1.86e-29	100
MS.gene70685.t1	AT2G21210	53.086	81	36	1	8	86	15	95	1.26e-28	99.0
MS.gene70685.t1	AT2G21210	53.086	81	36	1	8	86	82	162	8.43e-28	99.0
MS.gene70685.t1	AT4G34810	61.842	76	25	1	10	81	37	112	1.13e-27	97.1
MS.gene70685.t1	AT5G18080	58.108	74	30	1	14	86	17	90	2.00e-27	95.9
MS.gene70685.t1	AT4G38850	56.098	82	28	2	8	86	13	89	2.50e-27	95.5
MS.gene70685.t1	AT4G38825	57.692	78	27	2	10	86	17	89	8.52e-27	94.0
MS.gene70685.t1	AT2G21200	58.333	72	29	1	15	85	15	86	1.58e-26	93.2
MS.gene70685.t1	AT5G18020	60.000	70	27	1	14	82	17	86	1.59e-26	93.6
MS.gene70685.t1	AT5G18050	56.410	78	30	2	10	86	16	90	2.08e-26	93.2
MS.gene70685.t1	AT5G18060	62.121	66	24	1	22	86	25	90	2.56e-26	92.8
MS.gene70685.t1	AT5G18030	55.696	79	31	2	9	86	13	88	4.43e-26	92.4
MS.gene70685.t1	AT5G18010	55.405	74	32	1	14	86	17	90	1.11e-25	91.3
MS.gene70685.t1	AT4G13790	49.412	85	40	2	3	85	8	91	2.98e-25	90.1
MS.gene70685.t1	AT3G03820	53.165	79	34	1	11	86	17	95	2.18e-24	88.2
MS.gene70685.t1	AT3G03830	55.696	79	29	2	11	86	17	92	2.97e-24	87.8
MS.gene70685.t1	AT3G03850	61.194	67	24	1	22	86	26	92	5.04e-24	87.0
MS.gene70685.t1	AT4G34770	59.211	76	29	1	5	78	19	94	7.77e-24	87.0
MS.gene70685.t1	AT3G03840	52.564	78	35	1	11	86	17	94	3.61e-23	85.1
MS.gene70685.t1	AT4G38860	49.383	81	39	1	4	82	21	101	2.09e-22	83.6
MS.gene70685.t1	AT4G34790	57.143	70	26	2	21	86	39	108	2.15e-22	83.6
MS.gene70685.t1	AT2G21220	46.914	81	40	2	4	82	21	100	7.65e-21	79.3
MS.gene70685.t1	AT1G75580	55.224	67	29	1	19	85	42	107	1.14e-20	79.0
MS.gene70685.t1	AT4G34760	54.688	64	28	1	19	82	41	103	5.77e-20	77.4
MS.gene70685.t1	AT2G16580	54.688	64	28	1	19	82	42	104	1.34e-19	76.3
MS.gene70685.t1	AT4G34780	54.286	70	29	1	19	85	26	95	2.11e-19	75.9
MS.gene70685.t1	AT1G19830	58.333	60	24	1	19	78	46	104	2.41e-18	73.6
MS.gene70685.t1	AT4G36110	54.237	59	26	1	20	78	39	96	6.84e-18	72.0
MS.gene70685.t1	AT2G18010	54.237	59	26	1	20	78	47	104	1.98e-17	70.9
MS.gene70685.t1	AT3G43120	52.542	59	27	1	20	78	78	135	2.48e-16	69.3
MS.gene70685.t1	AT5G20810	54.237	59	26	1	20	78	78	135	1.18e-15	67.8
MS.gene70685.t1	AT3G20220	51.613	62	28	2	21	81	47	107	1.50e-15	66.2
MS.gene70685.t1	AT5G20810	54.237	59	26	1	20	78	78	135	1.78e-15	67.8
MS.gene70685.t1	AT5G66260	48.485	66	33	1	21	86	34	98	4.40e-14	62.0
MS.gene70685.t1	AT2G37030	49.206	63	31	1	20	82	48	109	2.78e-13	60.8
MS.gene70685.t1	AT3G51200	43.902	82	40	3	5	81	19	99	1.05e-12	58.9
MS.gene70685.t1	AT4G34750	43.548	62	34	1	20	81	36	96	1.14e-12	59.7
MS.gene70685.t1	AT4G34750	43.548	62	34	1	20	81	36	96	1.14e-12	59.7
MS.gene70685.t1	AT1G75590	47.458	59	30	1	20	78	41	98	1.27e-12	59.7
MS.gene70685.t1	AT5G10990	42.667	75	37	2	4	78	29	97	2.76e-12	58.5
MS.gene70685.t1	AT1G19840	44.000	75	36	2	4	78	29	97	5.32e-12	58.2
MS.gene70685.t1	AT3G53250	44.444	63	31	1	20	82	51	109	1.35e-11	56.2
MS.gene70685.t1	AT2G46690	41.096	73	39	2	5	74	8	79	1.59e-11	56.2
MS.gene70685.t1	AT4G31320	45.902	61	32	1	21	81	80	139	2.68e-11	57.0
MS.gene70685.t1	AT3G53250	44.444	63	31	1	20	82	82	140	3.33e-11	55.8
MS.gene70685.t1	AT3G61900	37.975	79	44	3	12	86	20	97	3.48e-11	55.5
MS.gene70685.t1	AT2G24400	47.541	61	31	1	21	81	64	123	9.44e-11	55.1
MS.gene70685.t1	AT2G45210	45.000	60	29	2	17	73	75	133	9.50e-11	55.1

Find 19 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGACTTGGCTCAACAATTCT+TGG	0.243513	3.3:+65381349	None:intergenic
GGCATCATCTTCAAAAGGTT+TGG	0.355226	3.3:-65381460	MS.gene70685:CDS
GCTCAACAATTCTTGGAATG+AGG	0.458184	3.3:+65381356	None:intergenic
TACACTGCTAGATAGCCTTT+CGG	0.463901	3.3:+65381432	None:intergenic
TACCTAGTATCATCAGATCA+AGG	0.482621	3.3:-65381482	MS.gene70685:CDS
ATTCAAATTGTTCCTCGACT+TGG	0.485458	3.3:+65381334	None:intergenic
TCAAGGGCATCATCTTCAAA+AGG	0.490872	3.3:-65381465	MS.gene70685:CDS
GCCCTTGATCTGATGATACT+AGG	0.523987	3.3:+65381480	None:intergenic
TTTGAATATGACCATCCAAT+GGG	0.542663	3.3:-65381318	MS.gene70685:CDS
AAGAAAATGTATTCTCTGCA+AGG	0.545012	3.3:+65381282	None:intergenic
ATTTGAATATGACCATCCAA+TGG	0.559485	3.3:-65381319	MS.gene70685:CDS
ACCTAGTATCATCAGATCAA+GGG	0.588343	3.3:-65381481	MS.gene70685:CDS
TAGTGAGACCACCCATTGGA+TGG	0.596413	3.3:+65381307	None:intergenic
GGAATAGTGAGACCACCCAT+TGG	0.603922	3.3:+65381303	None:intergenic
AAAGGTTTGGACGTGCCGAA+AGG	0.613661	3.3:-65381447	MS.gene70685:CDS
AGAATTGTTGAGCCAAGTCG+AGG	0.641143	3.3:-65381346	MS.gene70685:CDS
GAATATGACCATCCAATGGG+TGG	0.646056	3.3:-65381315	MS.gene70685:CDS
GGCTATCTAGCAGTGTACGT+AGG	0.679603	3.3:-65381426	MS.gene70685:CDS
ACGTAGGAGAGAAAATGAAG+CGG	0.744637	3.3:-65381410	MS.gene70685:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTGTTCAAATATGATATA+GGG	+	chr3.3:65381376-65381395	None:intergenic	15.0%
!!!	GTTTTGTTCAAATATGATAT+AGG	+	chr3.3:65381377-65381396	None:intergenic	20.0%
	AAGAAAATGTATTCTCTGCA+AGG	+	chr3.3:65381473-65381492	None:intergenic	30.0%
	ATTTGAATATGACCATCCAA+TGG	-	chr3.3:65381433-65381452	MS.gene70685:CDS	30.0%
	TTTGAATATGACCATCCAAT+GGG	-	chr3.3:65381434-65381453	MS.gene70685:CDS	30.0%
	ACCTAGTATCATCAGATCAA+GGG	-	chr3.3:65381271-65381290	MS.gene70685:CDS	35.0%
	ATTCAAATTGTTCCTCGACT+TGG	+	chr3.3:65381421-65381440	None:intergenic	35.0%
	TACCTAGTATCATCAGATCA+AGG	-	chr3.3:65381270-65381289	MS.gene70685:CDS	35.0%
	ACGTAGGAGAGAAAATGAAG+CGG	-	chr3.3:65381342-65381361	MS.gene70685:CDS	40.0%
	GCTCAACAATTCTTGGAATG+AGG	+	chr3.3:65381399-65381418	None:intergenic	40.0%
	GGCATCATCTTCAAAAGGTT+TGG	-	chr3.3:65381292-65381311	MS.gene70685:CDS	40.0%
	TACACTGCTAGATAGCCTTT+CGG	+	chr3.3:65381323-65381342	None:intergenic	40.0%
!!	TCAAGGGCATCATCTTCAAA+AGG	-	chr3.3:65381287-65381306	MS.gene70685:CDS	40.0%
	AGAATTGTTGAGCCAAGTCG+AGG	-	chr3.3:65381406-65381425	MS.gene70685:CDS	45.0%
	GAATATGACCATCCAATGGG+TGG	-	chr3.3:65381437-65381456	MS.gene70685:CDS	45.0%
	GCCCTTGATCTGATGATACT+AGG	+	chr3.3:65381275-65381294	None:intergenic	45.0%
!	CGACTTGGCTCAACAATTCT+TGG	+	chr3.3:65381406-65381425	None:intergenic	45.0%
	AAAGGTTTGGACGTGCCGAA+AGG	-	chr3.3:65381305-65381324	MS.gene70685:CDS	50.0%
	GGAATAGTGAGACCACCCAT+TGG	+	chr3.3:65381452-65381471	None:intergenic	50.0%
	GGCTATCTAGCAGTGTACGT+AGG	-	chr3.3:65381326-65381345	MS.gene70685:CDS	50.0%
	TAGTGAGACCACCCATTGGA+TGG	+	chr3.3:65381448-65381467	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	65381257	65381517	65381257	ID=MS.gene70685
chr3.3	mRNA	65381257	65381517	65381257	ID=MS.gene70685.t1;Parent=MS.gene70685
chr3.3	exon	65381257	65381517	65381257	ID=MS.gene70685.t1.exon1;Parent=MS.gene70685.t1
chr3.3	CDS	65381257	65381517	65381257	ID=cds.MS.gene70685.t1;Parent=MS.gene70685.t1

Gene Sequence	>MS.gene70685 ATGGGTTTTCGTTTACCTAGTATCATCAGATCAAGGGCATCATCTTCAAAAGGTTTGGACGTGCCGAAAGGCTATCTAGCAGTGTACGTAGGAGAGAAAATGAAGCGGTTTGTGATCCCTATATCATATTTGAACAAAACCTCATTCCAAGAATTGTTGAGCCAAGTCGAGGAACAATTTGAATATGACCATCCAATGGGTGGTCTCACTATTCCTTGCAGAGAATACATTTTCTTAGATATAACTTATCAGTTGAATTGA
Protein sequence	>MS.gene70685.t1 MGFRLPSIIRSRASSSKGLDVPKGYLAVYVGEKMKRFVIPISYLNKTSFQELLSQVEEQFEYDHPMGGLTIPCREYIFLDITYQLN