Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76428.t1 | RHN42073.1 | 96.2 | 105 | 4 | 0 | 1 | 105 | 7 | 111 | 1.10E-49 | 205.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76428.t1 | A0A396GNI7 | 96.2 | 105 | 4 | 0 | 1 | 105 | 7 | 111 | 8.1e-50 | 205.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene76428 | 0.824177 | 9.30E-54 | -1.69E-46 |
| MS.gene049351 | MS.gene76428 | 0.817583 | 3.06E-52 | -1.69E-46 |
| MS.gene049356 | MS.gene76428 | 0.808352 | 3.23E-50 | -1.69E-46 |
| MS.gene049427 | MS.gene76428 | 0.808742 | 2.67E-50 | -1.69E-46 |
| MS.gene049609 | MS.gene76428 | 0.822631 | 2.14E-53 | -1.69E-46 |
| MS.gene049805 | MS.gene76428 | 0.802333 | 5.90E-49 | -1.69E-46 |
| MS.gene050397 | MS.gene76428 | 0.805611 | 1.23E-49 | -1.69E-46 |
| MS.gene050400 | MS.gene76428 | 0.810549 | 1.09E-50 | -1.69E-46 |
| MS.gene050593 | MS.gene76428 | 0.833571 | 4.94E-56 | -1.69E-46 |
| MS.gene050670 | MS.gene76428 | 0.868386 | 6.79E-66 | -1.69E-46 |
| MS.gene050747 | MS.gene76428 | 0.806754 | 7.05E-50 | -1.69E-46 |
| MS.gene050772 | MS.gene76428 | 0.807875 | 4.08E-50 | -1.69E-46 |
| MS.gene050812 | MS.gene76428 | 0.804174 | 2.45E-49 | -1.69E-46 |
| MS.gene05081 | MS.gene76428 | 0.809873 | 1.53E-50 | -1.69E-46 |
| MS.gene050950 | MS.gene76428 | 0.803345 | 3.64E-49 | -1.69E-46 |
| MS.gene050971 | MS.gene76428 | 0.813604 | 2.35E-51 | -1.69E-46 |
| MS.gene05105 | MS.gene76428 | 0.868056 | 8.67E-66 | -1.69E-46 |
| MS.gene051255 | MS.gene76428 | 0.828986 | 6.63E-55 | -1.69E-46 |
| MS.gene051360 | MS.gene76428 | 0.829868 | 4.04E-55 | -1.69E-46 |
| MS.gene051387 | MS.gene76428 | 0.856438 | 3.22E-62 | -1.69E-46 |
| MS.gene051525 | MS.gene76428 | 0.859971 | 2.86E-63 | -1.69E-46 |
| MS.gene051605 | MS.gene76428 | 0.801864 | 7.36E-49 | -1.69E-46 |
| MS.gene051666 | MS.gene76428 | 0.801637 | 8.20E-49 | -1.69E-46 |
| MS.gene051692 | MS.gene76428 | 0.818311 | 2.09E-52 | -1.69E-46 |
| MS.gene051838 | MS.gene76428 | 0.802503 | 5.44E-49 | -1.69E-46 |
| MS.gene051858 | MS.gene76428 | 0.813013 | 3.17E-51 | -1.69E-46 |
| MS.gene052011 | MS.gene76428 | -0.821758 | 3.41E-53 | -1.69E-46 |
| MS.gene052188 | MS.gene76428 | 0.841979 | 3.42E-58 | -1.69E-46 |
| MS.gene052253 | MS.gene76428 | 0.859853 | 3.10E-63 | -1.69E-46 |
| MS.gene052310 | MS.gene76428 | 0.822755 | 2.00E-53 | -1.69E-46 |
| MS.gene052322 | MS.gene76428 | 0.836093 | 1.14E-56 | -1.69E-46 |
| MS.gene052340 | MS.gene76428 | 0.801404 | 9.15E-49 | -1.69E-46 |
| MS.gene052346 | MS.gene76428 | 0.843733 | 1.17E-58 | -1.69E-46 |
| MS.gene052414 | MS.gene76428 | 0.845028 | 5.24E-59 | -1.69E-46 |
| MS.gene052421 | MS.gene76428 | 0.831949 | 1.25E-55 | -1.69E-46 |
| MS.gene052481 | MS.gene76428 | 0.854535 | 1.15E-61 | -1.69E-46 |
| MS.gene052484 | MS.gene76428 | 0.838018 | 3.69E-57 | -1.69E-46 |
| MS.gene052485 | MS.gene76428 | 0.8691 | 3.99E-66 | -1.69E-46 |
| MS.gene052501 | MS.gene76428 | 0.824511 | 7.76E-54 | -1.69E-46 |
| MS.gene052505 | MS.gene76428 | 0.816859 | 4.45E-52 | -1.69E-46 |
| MS.gene052615 | MS.gene76428 | 0.809313 | 2.01E-50 | -1.69E-46 |
| MS.gene052982 | MS.gene76428 | 0.835941 | 1.25E-56 | -1.69E-46 |
| MS.gene052999 | MS.gene76428 | 0.814108 | 1.82E-51 | -1.69E-46 |
| MS.gene053169 | MS.gene76428 | 0.842721 | 2.18E-58 | -1.69E-46 |
| MS.gene053449 | MS.gene76428 | 0.817213 | 3.71E-52 | -1.69E-46 |
| MS.gene053574 | MS.gene76428 | 0.802457 | 5.56E-49 | -1.69E-46 |
| MS.gene053758 | MS.gene76428 | 0.836883 | 7.21E-57 | -1.69E-46 |
| MS.gene053813 | MS.gene76428 | 0.807777 | 4.28E-50 | -1.69E-46 |
| MS.gene054556 | MS.gene76428 | 0.842728 | 2.17E-58 | -1.69E-46 |
| MS.gene054719 | MS.gene76428 | 0.811774 | 5.92E-51 | -1.69E-46 |
| MS.gene05494 | MS.gene76428 | 0.801673 | 8.06E-49 | -1.69E-46 |
| MS.gene05505 | MS.gene76428 | 0.827371 | 1.62E-54 | -1.69E-46 |
| MS.gene055116 | MS.gene76428 | 0.829317 | 5.51E-55 | -1.69E-46 |
| MS.gene055206 | MS.gene76428 | 0.812456 | 4.20E-51 | -1.69E-46 |
| MS.gene055539 | MS.gene76428 | 0.813872 | 2.05E-51 | -1.69E-46 |
| MS.gene05553 | MS.gene76428 | 0.807408 | 5.13E-50 | -1.69E-46 |
| MS.gene055722 | MS.gene76428 | 0.802702 | 4.95E-49 | -1.69E-46 |
| MS.gene055741 | MS.gene76428 | 0.825548 | 4.41E-54 | -1.69E-46 |
| MS.gene055744 | MS.gene76428 | 0.829125 | 6.13E-55 | -1.69E-46 |
| MS.gene055909 | MS.gene76428 | 0.842878 | 1.98E-58 | -1.69E-46 |
| MS.gene055911 | MS.gene76428 | 0.84907 | 4.09E-60 | -1.69E-46 |
| MS.gene055941 | MS.gene76428 | 0.803673 | 3.12E-49 | -1.69E-46 |
| MS.gene056166 | MS.gene76428 | 0.806215 | 9.16E-50 | -1.69E-46 |
| MS.gene056191 | MS.gene76428 | 0.813869 | 2.06E-51 | -1.69E-46 |
| MS.gene056407 | MS.gene76428 | 0.817915 | 2.57E-52 | -1.69E-46 |
| MS.gene056410 | MS.gene76428 | 0.803877 | 2.83E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene76428 | 0.855691 | 5.32E-62 | -1.69E-46 |
| MS.gene056514 | MS.gene76428 | 0.804737 | 1.87E-49 | -1.69E-46 |
| MS.gene05666 | MS.gene76428 | 0.800644 | 1.31E-48 | -1.69E-46 |
| MS.gene056782 | MS.gene76428 | 0.808368 | 3.21E-50 | -1.69E-46 |
| MS.gene056796 | MS.gene76428 | 0.825164 | 5.44E-54 | -1.69E-46 |
| MS.gene056877 | MS.gene76428 | 0.822417 | 2.40E-53 | -1.69E-46 |
| MS.gene057019 | MS.gene76428 | 0.802109 | 6.56E-49 | -1.69E-46 |
| MS.gene057124 | MS.gene76428 | 0.810757 | 9.83E-51 | -1.69E-46 |
| MS.gene057146 | MS.gene76428 | 0.818555 | 1.84E-52 | -1.69E-46 |
| MS.gene057289 | MS.gene76428 | 0.809022 | 2.32E-50 | -1.69E-46 |
| MS.gene057416 | MS.gene76428 | 0.808418 | 3.13E-50 | -1.69E-46 |
| MS.gene057476 | MS.gene76428 | 0.80397 | 2.70E-49 | -1.69E-46 |
| MS.gene057734 | MS.gene76428 | 0.800923 | 1.15E-48 | -1.69E-46 |
| MS.gene057749 | MS.gene76428 | 0.859012 | 5.55E-63 | -1.69E-46 |
| MS.gene057823 | MS.gene76428 | 0.862426 | 5.11E-64 | -1.69E-46 |
| MS.gene057825 | MS.gene76428 | 0.800141 | 1.66E-48 | -1.69E-46 |
| MS.gene057856 | MS.gene76428 | 0.815304 | 9.90E-52 | -1.69E-46 |
| MS.gene058007 | MS.gene76428 | 0.850477 | 1.66E-60 | -1.69E-46 |
| MS.gene058019 | MS.gene76428 | 0.80361 | 3.21E-49 | -1.69E-46 |
| MS.gene05811 | MS.gene76428 | 0.829656 | 4.56E-55 | -1.69E-46 |
| MS.gene058376 | MS.gene76428 | 0.875138 | 3.90E-68 | -1.69E-46 |
| MS.gene058444 | MS.gene76428 | 0.859704 | 3.44E-63 | -1.69E-46 |
| MS.gene058660 | MS.gene76428 | 0.821898 | 3.16E-53 | -1.69E-46 |
| MS.gene058700 | MS.gene76428 | 0.857679 | 1.38E-62 | -1.69E-46 |
| MS.gene058928 | MS.gene76428 | 0.813741 | 2.20E-51 | -1.69E-46 |
| MS.gene058948 | MS.gene76428 | 0.829619 | 4.65E-55 | -1.69E-46 |
| MS.gene058995 | MS.gene76428 | 0.815758 | 7.85E-52 | -1.69E-46 |
| MS.gene05907 | MS.gene76428 | 0.846102 | 2.68E-59 | -1.69E-46 |
| MS.gene059082 | MS.gene76428 | 0.80813 | 3.60E-50 | -1.69E-46 |
| MS.gene059141 | MS.gene76428 | 0.837714 | 4.42E-57 | -1.69E-46 |
| MS.gene05934 | MS.gene76428 | 0.820162 | 7.94E-53 | -1.69E-46 |
| MS.gene059420 | MS.gene76428 | 0.848788 | 4.90E-60 | -1.69E-46 |
| MS.gene059455 | MS.gene76428 | 0.823816 | 1.13E-53 | -1.69E-46 |
| MS.gene059536 | MS.gene76428 | 0.81956 | 1.09E-52 | -1.69E-46 |
| MS.gene059674 | MS.gene76428 | 0.815638 | 8.34E-52 | -1.69E-46 |
| MS.gene059676 | MS.gene76428 | 0.815997 | 6.94E-52 | -1.69E-46 |
| MS.gene059688 | MS.gene76428 | 0.85326 | 2.69E-61 | -1.69E-46 |
| MS.gene059692 | MS.gene76428 | 0.828455 | 8.91E-55 | -1.69E-46 |
| MS.gene059695 | MS.gene76428 | 0.833905 | 4.08E-56 | -1.69E-46 |
| MS.gene059902 | MS.gene76428 | 0.808922 | 2.44E-50 | -1.69E-46 |
| MS.gene060403 | MS.gene76428 | 0.851054 | 1.14E-60 | -1.69E-46 |
| MS.gene060469 | MS.gene76428 | 0.80416 | 2.47E-49 | -1.69E-46 |
| MS.gene060485 | MS.gene76428 | 0.815812 | 7.63E-52 | -1.69E-46 |
| MS.gene060804 | MS.gene76428 | 0.825824 | 3.80E-54 | -1.69E-46 |
| MS.gene060835 | MS.gene76428 | 0.805599 | 1.24E-49 | -1.69E-46 |
| MS.gene060837 | MS.gene76428 | 0.865156 | 7.24E-65 | -1.69E-46 |
| MS.gene060986 | MS.gene76428 | 0.83028 | 3.21E-55 | -1.69E-46 |
| MS.gene061154 | MS.gene76428 | 0.828415 | 9.11E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76428.t1 | MTR_8g077115 | 96.154 | 104 | 4 | 0 | 1 | 104 | 7 | 110 | 3.71e-71 | 214 |
| MS.gene76428.t1 | MTR_8g077120 | 65.385 | 104 | 36 | 0 | 1 | 104 | 1 | 104 | 9.83e-45 | 145 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTTGTTCCTTTCAATCTC+TGG | 0.215633 | 8.1:-30642984 | MS.gene76428:CDS |
| CTTCAAGATCATTCACTTGT+GGG | 0.325356 | 8.1:-30643005 | MS.gene76428:CDS |
| AACAAGAAGCTTTCGTTGAT+AGG | 0.397043 | 8.1:-30642885 | MS.gene76428:CDS |
| GCTTGTTCCTTTCAATCTCT+GGG | 0.399249 | 8.1:-30642983 | MS.gene76428:CDS |
| GTGGTAATTTAGGAAGGAAG+TGG | 0.454585 | 8.1:+30642793 | None:intergenic |
| GCTTCAAGATCATTCACTTG+TGG | 0.509536 | 8.1:-30643006 | MS.gene76428:CDS |
| TAAGAGTTGATTCCACGTCC+TGG | 0.534079 | 8.1:+30642833 | None:intergenic |
| GTAAGAACTGCTCAGAGAGA+TGG | 0.542961 | 8.1:-30642937 | MS.gene76428:CDS |
| CAGAGAGATGGAAAGAGTGA+TGG | 0.564058 | 8.1:-30642925 | MS.gene76428:CDS |
| ATAGCAAAGCGTGGCTGTTG+CGG | 0.565629 | 8.1:+30643110 | None:intergenic |
| GGAATCAACTCTTAATCGAC+TGG | 0.570438 | 8.1:-30642824 | MS.gene76428:CDS |
| CATGGAGTGGTAATTTAGGA+AGG | 0.580532 | 8.1:+30642787 | None:intergenic |
| GATCTTGAAGCAGATAAACT+AGG | 0.591700 | 8.1:+30643018 | None:intergenic |
| TGGGTGAAAAGTGCGATGGA+TGG | 0.619297 | 8.1:-30642964 | MS.gene76428:CDS |
| TCAACTCTTAATCGACTGGT+CGG | 0.626805 | 8.1:-30642820 | MS.gene76428:CDS |
| TCTCTGGGTGAAAAGTGCGA+TGG | 0.680175 | 8.1:-30642968 | MS.gene76428:CDS |
| AGATGGATTATAGCAAAGCG+TGG | 0.685105 | 8.1:+30643101 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTCAAGTATCTTTGTTT+AGG | + | chr8.1:30642864-30642883 | None:intergenic | 20.0% |
| !!! | ATCCATCTAACAATTTTTTG+AGG | - | chr8.1:30642829-30642848 | MS.gene76428:CDS | 25.0% |
| GTCCTCAAAAAATTGTTAGA+TGG | + | chr8.1:30642834-30642853 | None:intergenic | 30.0% | |
| AACAAGAAGCTTTCGTTGAT+AGG | - | chr8.1:30643030-30643049 | MS.gene76428:CDS | 35.0% | |
| CTTCAAGATCATTCACTTGT+GGG | - | chr8.1:30642910-30642929 | MS.gene76428:CDS | 35.0% | |
| GATCTTGAAGCAGATAAACT+AGG | + | chr8.1:30642900-30642919 | None:intergenic | 35.0% | |
| ACGTCCTGGAACAAAAACTT+TGG | + | chr8.1:30643071-30643090 | None:intergenic | 40.0% | |
| GCTTCAAGATCATTCACTTG+TGG | - | chr8.1:30642909-30642928 | MS.gene76428:CDS | 40.0% | |
| GCTTGTTCCTTTCAATCTCT+GGG | - | chr8.1:30642932-30642951 | MS.gene76428:CDS | 40.0% | |
| GGAATCAACTCTTAATCGAC+TGG | - | chr8.1:30643091-30643110 | MS.gene76428:CDS | 40.0% | |
| TCAACTCTTAATCGACTGGT+CGG | - | chr8.1:30643095-30643114 | MS.gene76428:CDS | 40.0% | |
| ! | AAAGTTTTTGTTCCAGGACG+TGG | - | chr8.1:30643070-30643089 | MS.gene76428:CDS | 40.0% |
| ! | CTTTTCACCCAGAGATTGAA+AGG | + | chr8.1:30642942-30642961 | None:intergenic | 40.0% |
| ! | TGAGCCAAAGTTTTTGTTCC+AGG | - | chr8.1:30643064-30643083 | MS.gene76428:CDS | 40.0% |
| !! | AGATGGATTATAGCAAAGCG+TGG | + | chr8.1:30642817-30642836 | None:intergenic | 40.0% |
| CAGAGAGATGGAAAGAGTGA+TGG | - | chr8.1:30642990-30643009 | MS.gene76428:CDS | 45.0% | |
| GTAAGAACTGCTCAGAGAGA+TGG | - | chr8.1:30642978-30642997 | MS.gene76428:CDS | 45.0% | |
| ! | GGCTTGTTCCTTTCAATCTC+TGG | - | chr8.1:30642931-30642950 | MS.gene76428:CDS | 45.0% |
| ! | TAAGAGTTGATTCCACGTCC+TGG | + | chr8.1:30643085-30643104 | None:intergenic | 45.0% |
| ATAGCAAAGCGTGGCTGTTG+CGG | + | chr8.1:30642808-30642827 | None:intergenic | 50.0% | |
| ! | TCTCTGGGTGAAAAGTGCGA+TGG | - | chr8.1:30642947-30642966 | MS.gene76428:CDS | 50.0% |
| ! | TGGGTGAAAAGTGCGATGGA+TGG | - | chr8.1:30642951-30642970 | MS.gene76428:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 30642801 | 30643136 | 30642801 | ID=MS.gene76428 |
| chr8.1 | mRNA | 30642801 | 30643136 | 30642801 | ID=MS.gene76428.t1;Parent=MS.gene76428 |
| chr8.1 | exon | 30642801 | 30643136 | 30642801 | ID=MS.gene76428.t1.exon1;Parent=MS.gene76428.t1 |
| chr8.1 | CDS | 30642801 | 30643136 | 30642801 | ID=cds.MS.gene76428.t1;Parent=MS.gene76428.t1 |
| Gene Sequence |
| Protein sequence |
