AlfalfaGEDB | MS.gene77525

M. sativa cultivar XinJiangDaYe / MS.gene77525

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77525.t1	AES71926.1	93.5	92	6	0	1	92	1	92	4.20E-42	180.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77525.t1	P33080	75.0	92	23	0	1	92	1	92	2.8e-34	145.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77525.t1	G7JAQ9	93.5	92	6	0	1	92	1	92	3.0e-42	180.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049758	MS.gene77525	0.801507	8.71E-49	-1.69E-46
MS.gene049915	MS.gene77525	0.802197	6.29E-49	-1.69E-46
MS.gene053836	MS.gene77525	0.822947	1.80E-53	-1.69E-46
MS.gene054473	MS.gene77525	0.80618	9.32E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene77525	MS.gene68163	PPI
MS.gene003591	MS.gene77525	PPI
MS.gene062979	MS.gene77525	PPI
MS.gene77525	MS.gene057818	PPI
MS.gene062943	MS.gene77525	PPI
MS.gene77525	MS.gene79889	PPI
MS.gene77525	MS.gene045368	PPI
MS.gene01358	MS.gene77525	PPI
MS.gene003608	MS.gene77525	PPI
MS.gene77525	MS.gene045360	PPI
MS.gene77525	MS.gene014222	PPI
MS.gene40326	MS.gene77525	PPI
MS.gene77525	MS.gene75678	PPI
MS.gene77525	MS.gene75314	PPI
MS.gene77525	MS.gene01764	PPI
MS.gene77525	MS.gene031199	PPI
MS.gene50676	MS.gene77525	PPI
MS.gene77525	MS.gene75673	PPI
MS.gene23370	MS.gene77525	PPI
MS.gene50766	MS.gene77525	PPI
MS.gene003594	MS.gene77525	PPI
MS.gene50665	MS.gene77525	PPI
MS.gene77525	MS.gene031198	PPI
MS.gene003625	MS.gene77525	PPI
MS.gene77525	MS.gene50764	PPI
MS.gene77525	MS.gene003577	PPI
MS.gene77525	MS.gene75672	PPI
MS.gene77525	MS.gene75674	PPI
MS.gene50763	MS.gene77525	PPI
MS.gene77525	MS.gene50654	PPI
MS.gene77525	MS.gene50675	PPI
MS.gene045365	MS.gene77525	PPI
MS.gene77525	MS.gene014670	PPI
MS.gene77525	MS.gene77524	PPI
MS.gene50659	MS.gene77525	PPI
MS.gene77525	MS.gene68162	PPI
MS.gene75316	MS.gene77525	PPI
MS.gene77525	MS.gene79887	PPI
MS.gene057790	MS.gene77525	PPI
MS.gene062952	MS.gene77525	PPI
MS.gene003624	MS.gene77525	PPI
MS.gene77525	MS.gene75679	PPI
MS.gene77525	MS.gene68761	PPI
MS.gene77525	MS.gene40325	PPI
MS.gene77529	MS.gene77525	PPI
MS.gene014667	MS.gene77525	PPI
MS.gene77525	MS.gene50677	PPI
MS.gene77525	MS.gene68760	PPI
MS.gene77525	MS.gene50684	PPI
MS.gene77525	MS.gene057797	PPI
MS.gene77525	MS.gene003614	PPI
MS.gene77525	MS.gene014668	PPI
MS.gene057778	MS.gene77525	PPI
MS.gene77525	MS.gene77528	PPI
MS.gene77525	MS.gene79886	PPI
MS.gene77525	MS.gene014671	PPI
MS.gene77525	MS.gene50765	PPI
MS.gene77525	MS.gene003596	PPI
MS.gene77525	MS.gene062988	PPI
MS.gene77525	MS.gene23371	PPI
MS.gene77525	MS.gene062974	PPI
MS.gene003615	MS.gene77525	PPI
MS.gene77525	MS.gene003583	PPI
MS.gene77525	MS.gene062951	PPI
MS.gene77525	MS.gene057801	PPI
MS.gene77525	MS.gene77527	PPI
MS.gene77525	MS.gene79891	PPI
MS.gene003602	MS.gene77525	PPI
MS.gene77525	MS.gene057795	PPI
MS.gene77525	MS.gene045367	PPI
MS.gene77525	MS.gene045364	PPI
MS.gene77525	MS.gene045363	PPI
MS.gene77525	MS.gene75675	PPI
MS.gene062984	MS.gene77525	PPI
MS.gene77525	MS.gene50652	PPI
MS.gene40327	MS.gene77525	PPI
MS.gene77525	MS.gene002380	PPI
MS.gene031201	MS.gene77525	PPI
MS.gene77525	MS.gene68165	PPI
MS.gene77525	MS.gene77526	PPI
MS.gene77525	MS.gene045366	PPI
MS.gene77525	MS.gene045362	PPI
MS.gene77523	MS.gene77525	PPI
MS.gene77525	MS.gene23372	PPI
MS.gene003587	MS.gene77525	PPI
MS.gene77525	MS.gene75315	PPI
MS.gene77525	MS.gene68164	PPI
MS.gene003590	MS.gene77525	PPI
MS.gene063112	MS.gene77525	PPI
MS.gene77525	MS.gene68759	PPI
MS.gene77525	MS.gene003586	PPI
MS.gene77525	MS.gene003627	PPI
MS.gene77525	MS.gene031200	PPI
MS.gene79890	MS.gene77525	PPI
MS.gene77525	MS.gene75676	PPI
MS.gene062973	MS.gene77525	PPI
MS.gene77525	MS.gene79888	PPI
MS.gene047517	MS.gene77525	PPI
MS.gene77525	MS.gene50687	PPI
MS.gene77525	MS.gene014223	PPI
MS.gene77525	MS.gene79885	PPI
MS.gene77525	MS.gene014669	PPI
MS.gene77525	MS.gene057808	PPI
MS.gene75677	MS.gene77525	PPI
MS.gene77525	MS.gene062963	PPI
MS.gene045361	MS.gene77525	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77525.t1	MTR_3g084230	93.478	92	6	0	1	92	1	92	2.21e-55	166
MS.gene77525.t1	MTR_3g084190	93.478	92	6	0	1	92	1	92	4.97e-55	165
MS.gene77525.t1	MTR_8g461420	91.304	92	8	0	1	92	1	92	1.98e-54	164
MS.gene77525.t1	MTR_3g084160	93.478	92	6	0	1	92	1	92	4.72e-54	163
MS.gene77525.t1	MTR_3g084250	83.696	92	15	0	1	92	1	92	2.24e-53	161
MS.gene77525.t1	MTR_3g084210	89.130	92	10	0	1	92	1	92	7.18e-53	160
MS.gene77525.t1	MTR_3g110045	86.667	90	12	0	3	92	62	151	7.48e-49	152
MS.gene77525.t1	MTR_4g072500	70.652	92	27	0	1	92	1	92	1.35e-44	139
MS.gene77525.t1	MTR_3g084150	69.565	92	28	0	1	92	1	92	1.92e-44	139
MS.gene77525.t1	MTR_8g461410	68.478	92	29	0	1	92	1	92	1.43e-43	136
MS.gene77525.t1	MTR_3g084180	68.478	92	29	0	1	92	1	92	2.10e-43	136
MS.gene77525.t1	MTR_4g072530	70.000	90	27	0	1	90	1	90	2.57e-43	135
MS.gene77525.t1	MTR_4g072480	68.478	92	29	0	1	92	1	92	9.12e-43	134
MS.gene77525.t1	MTR_4g072640	69.231	91	28	0	1	91	1	91	1.14e-42	134
MS.gene77525.t1	MTR_4g072310	67.742	93	29	1	1	92	1	93	2.91e-42	133
MS.gene77525.t1	MTR_3g110042	71.264	87	25	0	1	87	1	87	3.64e-42	133
MS.gene77525.t1	MTR_4g072880	67.742	93	29	1	1	92	1	93	4.46e-42	132
MS.gene77525.t1	MTR_3g084170	72.043	93	24	2	1	92	1	92	4.94e-42	132
MS.gene77525.t1	MTR_4g050700	68.817	93	28	1	1	92	1	93	6.99e-42	132
MS.gene77525.t1	MTR_2g012410	68.817	93	28	1	1	92	1	93	6.99e-42	132
MS.gene77525.t1	MTR_4g072270	67.742	93	29	1	1	92	1	93	1.03e-41	132
MS.gene77525.t1	MTR_4g072870	70.330	91	26	1	1	90	1	91	1.10e-41	132
MS.gene77525.t1	MTR_4g072620	66.304	92	31	0	1	92	1	92	1.69e-41	131
MS.gene77525.t1	MTR_4g072240	69.231	91	27	1	1	90	1	91	3.81e-41	130
MS.gene77525.t1	MTR_3g084240	70.968	93	25	2	1	92	1	92	3.88e-41	130
MS.gene77525.t1	MTR_4g072570	66.304	92	31	0	1	92	1	92	5.76e-41	130
MS.gene77525.t1	MTR_4g072680	66.304	92	31	0	1	92	1	92	6.50e-41	130
MS.gene77525.t1	MTR_4g072730	66.667	90	30	0	1	90	1	90	7.25e-41	129
MS.gene77525.t1	MTR_4g072370	67.778	90	27	1	1	90	1	88	9.61e-41	129
MS.gene77525.t1	MTR_4g072600	69.231	91	23	1	1	91	1	86	1.47e-40	129
MS.gene77525.t1	MTR_3g117630	70.588	85	25	0	8	92	8	92	1.50e-40	129
MS.gene77525.t1	MTR_4g072220	67.742	93	29	1	1	92	1	93	1.98e-40	129
MS.gene77525.t1	MTR_4g072230	67.742	93	27	2	1	92	1	91	3.13e-40	128
MS.gene77525.t1	MTR_4g072300	65.934	91	30	1	1	90	1	91	8.13e-40	127
MS.gene77525.t1	MTR_4g072830	66.304	92	29	1	1	92	1	90	8.81e-40	127
MS.gene77525.t1	MTR_4g072890	67.033	91	29	1	1	90	1	91	1.09e-39	127
MS.gene77525.t1	MTR_4g072330	64.444	90	30	1	1	90	1	88	1.10e-39	127
MS.gene77525.t1	MTR_4g072610	68.132	91	23	1	1	91	1	85	1.34e-39	126
MS.gene77525.t1	MTR_3g110038	68.817	93	27	2	1	92	1	92	1.46e-39	126
MS.gene77525.t1	MTR_4g072860	64.516	93	32	1	1	92	1	93	1.70e-39	126
MS.gene77525.t1	MTR_4g072580	67.033	91	25	1	1	91	1	86	1.96e-39	126
MS.gene77525.t1	MTR_4g072460	64.130	92	33	0	1	92	1	92	2.13e-39	126
MS.gene77525.t1	MTR_4g072260	65.591	93	31	1	1	92	1	93	2.17e-39	126
MS.gene77525.t1	MTR_3g110032	65.591	93	31	1	1	92	1	93	2.31e-39	126
MS.gene77525.t1	MTR_3g084220	65.217	92	32	0	1	92	1	92	2.43e-39	125
MS.gene77525.t1	MTR_4g072490	67.778	90	24	1	1	90	1	85	2.90e-39	125
MS.gene77525.t1	MTR_4g072250	69.231	91	26	2	1	90	1	90	5.46e-39	125
MS.gene77525.t1	MTR_4g072630	68.889	90	24	1	1	90	1	86	8.10e-39	124
MS.gene77525.t1	MTR_4g072740	67.778	90	24	1	1	90	1	85	1.17e-38	124
MS.gene77525.t1	MTR_4g072650	68.132	91	23	1	1	91	1	85	1.37e-38	124
MS.gene77525.t1	MTR_4g072850	68.085	94	28	1	1	92	1	94	1.59e-38	124
MS.gene77525.t1	MTR_4g072820	68.889	90	23	1	1	90	1	85	2.40e-38	123
MS.gene77525.t1	MTR_4g072540	67.778	90	25	1	1	90	1	86	8.30e-37	119
MS.gene77525.t1	MTR_4g072470	64.444	90	27	1	1	90	1	85	4.98e-36	117
MS.gene77525.t1	MTR_4g072465	63.333	90	29	1	1	90	1	86	5.35e-36	117
MS.gene77525.t1	MTR_4g072380	65.556	90	27	1	1	90	1	86	1.34e-35	116
MS.gene77525.t1	MTR_3g084260	63.736	91	32	1	1	90	1	91	1.54e-35	116
MS.gene77525.t1	MTR_4g072590	66.667	90	25	1	1	90	1	85	1.78e-35	116
MS.gene77525.t1	MTR_4g072660	61.957	92	30	1	1	92	1	87	2.45e-35	115
MS.gene77525.t1	MTR_4g072720	67.778	90	25	1	1	90	1	86	4.02e-35	115
MS.gene77525.t1	MTR_4g072940	66.304	92	29	1	3	92	33	124	4.87e-35	116
MS.gene77525.t1	MTR_4g072510	65.556	90	27	1	1	90	1	86	1.19e-34	114
MS.gene77525.t1	MTR_4g072670	63.333	90	29	1	1	90	1	86	1.34e-34	114
MS.gene77525.t1	MTR_5g008360	62.195	82	31	0	11	92	2	83	2.28e-33	110
MS.gene77525.t1	MTR_4g072980	57.609	92	36	2	1	89	1	92	2.61e-32	108
MS.gene77525.t1	MTR_5g008370	58.889	90	37	0	1	90	1	90	3.08e-32	109
MS.gene77525.t1	MTR_4g072450	63.333	90	28	1	1	90	1	85	6.06e-32	107
MS.gene77525.t1	MTR_4g072690	63.333	90	28	1	1	90	1	85	2.09e-31	105
MS.gene77525.t1	MTR_4g072910	57.831	83	35	0	8	90	18	100	5.29e-31	105
MS.gene77525.t1	MTR_3g110035	75.758	66	16	0	27	92	73	138	1.53e-30	105
MS.gene77525.t1	MTR_4g072770	56.667	90	29	1	1	90	1	80	5.46e-30	102
MS.gene77525.t1	MTR_4g072550	59.302	86	34	1	7	92	19	103	8.93e-30	102
MS.gene77525.t1	MTR_3g084200	68.493	73	21	2	1	72	1	72	1.35e-29	101
MS.gene77525.t1	MTR_5g008350	60.759	79	26	1	1	79	1	74	2.19e-29	100
MS.gene77525.t1	MTR_4g072280	60.000	75	29	1	1	74	1	75	2.54e-28	97.8
MS.gene77525.t1	MTR_4g072750	62.500	80	28	1	1	78	1	80	3.05e-28	97.8
MS.gene77525.t1	MTR_4g072560	51.546	97	39	3	1	89	1	97	7.57e-28	97.4
MS.gene77525.t1	MTR_3g117640	63.235	68	24	1	24	90	33	100	5.24e-27	95.1
MS.gene77525.t1	MTR_4g072290	54.839	93	27	2	1	92	1	79	2.78e-26	92.8
MS.gene77525.t1	MTR_3g117610	51.087	92	36	2	8	91	20	110	7.22e-25	90.1
MS.gene77525.t1	MTR_3g113310	58.824	68	27	1	22	89	39	105	6.31e-24	87.4
MS.gene77525.t1	MTR_4g072930	50.617	81	34	2	16	90	30	110	5.24e-23	85.5
MS.gene77525.t1	MTR_4g072190	58.209	67	27	1	23	89	34	99	2.08e-22	83.6
MS.gene77525.t1	MTR_6g077850	50.000	68	34	0	24	91	33	100	2.17e-22	83.6
MS.gene77525.t1	MTR_5g021820	47.674	86	40	2	8	89	19	103	9.48e-21	79.3
MS.gene77525.t1	MTR_8g076040	56.061	66	28	1	24	89	40	104	2.87e-20	78.2
MS.gene77525.t1	MTR_8g461400	47.826	92	22	1	1	92	1	66	4.21e-20	76.6
MS.gene77525.t1	MTR_4g025830	59.016	61	21	2	25	82	79	138	5.61e-20	79.0
MS.gene77525.t1	MTR_4g072840	50.549	91	13	3	1	90	1	60	2.63e-19	74.3
MS.gene77525.t1	MTR_4g072160	51.471	68	32	1	24	91	36	102	4.27e-18	73.6
MS.gene77525.t1	MTR_1g024085	46.753	77	38	2	8	82	18	93	8.07e-18	73.2
MS.gene77525.t1	MTR_1g063950	46.667	75	39	1	9	83	32	105	2.16e-17	71.2
MS.gene77525.t1	MTR_4g124750	43.182	88	47	2	4	89	46	132	2.36e-17	72.0
MS.gene77525.t1	MTR_0388s0020	50.000	70	31	2	12	78	62	130	3.75e-17	71.6
MS.gene77525.t1	MTR_1g094750	47.368	76	37	2	11	84	34	108	8.51e-17	69.7
MS.gene77525.t1	MTR_2g043960	51.429	70	33	1	15	84	66	134	1.08e-16	70.5
MS.gene77525.t1	MTR_8g461210	42.857	84	47	1	8	91	18	100	1.35e-16	69.3
MS.gene77525.t1	MTR_7g101740	46.753	77	40	1	11	87	26	101	2.04e-16	68.6
MS.gene77525.t1	MTR_1g061570	53.030	66	28	2	19	82	11	75	4.05e-16	68.2
MS.gene77525.t1	MTR_4g005310	48.611	72	34	2	13	82	50	120	1.93e-15	67.0
MS.gene77525.t1	MTR_7g118010	46.377	69	34	2	25	91	23	90	2.10e-15	65.9
MS.gene77525.t1	MTR_1g009150	48.529	68	34	1	15	82	61	127	2.24e-14	65.1
MS.gene77525.t1	MTR_3g092220	44.928	69	35	2	16	82	24	91	7.67e-14	62.0
MS.gene77525.t1	MTR_8g026730	43.478	69	36	2	25	91	27	94	1.71e-13	60.8
MS.gene77525.t1	MTR_4g005320	47.692	65	33	1	25	89	70	133	2.61e-13	62.0
MS.gene77525.t1	MTR_3g097120	43.836	73	32	2	20	84	4	75	1.28e-12	58.9
MS.gene77525.t1	MTR_3g098970	34.483	87	51	2	11	92	34	119	2.77e-12	58.9
MS.gene77525.t1	MTR_7g405740	39.241	79	40	3	5	82	52	123	2.92e-12	58.9
MS.gene77525.t1	MTR_3g109160	44.595	74	40	1	9	82	53	125	3.02e-12	59.3
MS.gene77525.t1	MTR_2g066830	36.842	76	41	2	2	77	41	109	1.66e-11	56.6
MS.gene77525.t1	MTR_8g096470	39.394	66	39	1	26	91	47	111	3.58e-11	55.1
MS.gene77525.t1	MTR_8g101520	46.269	67	32	2	26	92	51	113	5.13e-11	55.8
MS.gene77525.t1	MTR_8g096500	44.828	58	31	1	25	82	49	105	9.53e-11	55.1
MS.gene77525.t1	MTR_8g096440	40.845	71	39	2	12	82	34	101	9.62e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77525.t1	AT2G21200	65.278	72	24	1	19	89	15	86	6.79e-29	99.8
MS.gene77525.t1	AT4G34800	60.274	73	26	1	14	83	10	82	1.28e-28	99.0
MS.gene77525.t1	AT5G18020	57.143	77	32	1	16	91	15	91	1.29e-28	99.0
MS.gene77525.t1	AT4G34770	51.961	102	37	3	1	90	1	102	1.50e-28	99.4
MS.gene77525.t1	AT4G38840	63.235	68	24	1	24	90	32	99	2.18e-28	98.6
MS.gene77525.t1	AT2G21210	60.811	74	27	1	19	90	22	95	2.34e-28	98.6
MS.gene77525.t1	AT3G03820	56.098	82	33	1	9	87	11	92	5.85e-28	97.4
MS.gene77525.t1	AT5G18080	59.211	76	30	1	16	90	15	90	5.89e-28	97.4
MS.gene77525.t1	AT2G21210	60.811	74	27	1	19	90	89	162	9.07e-28	99.0
MS.gene77525.t1	AT4G34810	55.422	83	30	2	8	83	28	110	1.38e-27	97.1
MS.gene77525.t1	AT4G38850	57.831	83	22	2	8	87	14	86	1.66e-27	96.3
MS.gene77525.t1	AT5G18050	56.579	76	32	1	16	90	15	90	2.37e-27	95.9
MS.gene77525.t1	AT5G18010	56.579	76	32	1	16	90	15	90	2.45e-27	95.9
MS.gene77525.t1	AT5G18060	57.895	76	31	1	16	90	15	90	4.05e-27	95.1
MS.gene77525.t1	AT4G38825	58.667	75	30	1	17	90	15	89	9.14e-27	94.4
MS.gene77525.t1	AT3G03840	53.933	89	28	2	1	87	14	91	1.22e-26	94.0
MS.gene77525.t1	AT5G18030	51.765	85	35	2	7	90	9	88	2.00e-26	93.2
MS.gene77525.t1	AT3G03850	65.672	67	21	1	26	90	26	92	8.36e-26	92.0
MS.gene77525.t1	AT4G38860	61.765	68	26	0	23	90	38	105	3.67e-25	90.5
MS.gene77525.t1	AT4G34790	61.538	65	23	1	25	87	39	103	5.30e-25	90.5
MS.gene77525.t1	AT4G13790	53.521	71	31	1	19	87	19	89	2.86e-23	85.5
MS.gene77525.t1	AT4G34780	55.556	72	29	1	23	91	26	97	6.69e-23	85.1
MS.gene77525.t1	AT2G21220	50.000	86	38	2	8	89	19	103	9.50e-23	84.7
MS.gene77525.t1	AT1G75580	61.194	67	25	1	23	89	42	107	2.13e-22	84.0
MS.gene77525.t1	AT3G03830	56.164	73	29	1	18	87	17	89	3.24e-22	82.8
MS.gene77525.t1	AT4G34760	58.209	67	27	1	23	89	41	106	1.49e-21	81.6
MS.gene77525.t1	AT1G19830	61.667	60	22	1	23	82	46	104	1.34e-19	77.0
MS.gene77525.t1	AT2G16580	55.224	67	29	1	23	89	42	107	7.33e-19	74.7
MS.gene77525.t1	AT5G66260	48.235	85	41	2	8	90	15	98	1.82e-18	73.6
MS.gene77525.t1	AT3G43120	52.174	69	32	1	14	82	68	135	2.25e-18	75.1
MS.gene77525.t1	AT5G20810	54.545	66	29	1	17	82	71	135	1.56e-17	72.8
MS.gene77525.t1	AT4G36110	55.932	59	25	1	24	82	39	96	1.96e-17	70.9
MS.gene77525.t1	AT5G20810	54.545	66	29	1	17	82	71	135	2.12e-17	73.2
MS.gene77525.t1	AT1G75590	46.835	79	37	2	8	82	21	98	2.79e-17	72.0
MS.gene77525.t1	AT2G45210	55.357	56	21	2	25	77	79	133	3.57e-17	72.0
MS.gene77525.t1	AT3G20220	47.887	71	35	2	13	82	35	104	4.77e-17	70.5
MS.gene77525.t1	AT2G18010	55.932	59	25	1	24	82	47	104	6.64e-17	70.1
MS.gene77525.t1	AT4G34750	44.118	68	37	1	24	91	36	102	8.35e-17	70.5
MS.gene77525.t1	AT4G34750	44.118	68	37	1	24	91	36	102	8.35e-17	70.5
MS.gene77525.t1	AT5G10990	40.230	87	48	2	8	91	21	106	1.08e-16	70.5
MS.gene77525.t1	AT1G19840	46.154	78	38	2	8	82	21	97	2.85e-16	69.3
MS.gene77525.t1	AT2G37030	49.206	63	31	1	24	86	48	109	3.27e-16	68.6
MS.gene77525.t1	AT3G61900	41.176	85	46	2	10	91	15	98	1.59e-15	67.0
MS.gene77525.t1	AT3G60690	52.459	61	25	2	25	82	88	147	4.11e-15	66.6
MS.gene77525.t1	AT4G00880	48.571	70	33	2	24	91	27	95	9.23e-15	64.7
MS.gene77525.t1	AT3G51200	44.156	77	39	3	8	83	24	97	4.00e-14	62.8
MS.gene77525.t1	AT5G53590	44.444	72	34	2	24	90	45	115	9.13e-14	62.8
MS.gene77525.t1	AT2G46690	40.260	77	42	2	17	90	16	91	1.03e-13	62.0
MS.gene77525.t1	AT3G53250	42.857	63	32	1	24	86	51	109	1.57e-13	61.6
MS.gene77525.t1	AT4G09530	45.714	70	32	3	16	82	22	88	1.80e-13	60.8
MS.gene77525.t1	AT3G12830	45.588	68	35	2	11	77	35	101	2.06e-13	61.6
MS.gene77525.t1	AT3G53250	42.857	63	32	1	24	86	82	140	2.64e-13	61.6
MS.gene77525.t1	AT4G12410	42.500	80	41	3	6	82	58	135	3.77e-13	61.2
MS.gene77525.t1	AT4G22620	42.500	80	37	3	6	82	65	138	1.22e-12	60.1
MS.gene77525.t1	AT1G56150	46.552	58	30	1	25	82	38	94	2.78e-12	58.2
MS.gene77525.t1	AT1G16510	44.828	58	31	1	25	82	54	110	6.92e-12	57.8
MS.gene77525.t1	AT4G31320	46.552	58	30	1	25	82	80	136	7.34e-11	55.8
MS.gene77525.t1	AT3G09870	49.123	57	26	2	24	78	44	99	7.48e-11	54.3
MS.gene77525.t1	AT2G24400	48.276	58	29	1	25	82	64	120	9.04e-11	55.5

Find 22 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAAGAACATGTCTTCCTTGC+AGG	0.295055	3.2:-72563269	None:intergenic
CTAAGCAATTCTTGAAATAA+AGG	0.317640	3.2:-72563193	None:intergenic
TACACTGCAAGATGGCCTTT+TGG	0.383305	3.2:-72563118	None:intergenic
TTCGCTTACCTGGTATTAGA+AGG	0.386157	3.2:+72563050	MS.gene77525:CDS
TCAATCATCTAGCAAACAAC+TGG	0.425756	3.2:+72563090	MS.gene77525:CDS
ATACAACTTCTGTCTTGAAC+AGG	0.433943	3.2:+72563293	MS.gene77525:CDS
AGCCAGGCTGAAGAGGAATT+CGG	0.463119	3.2:+72563214	MS.gene77525:CDS
ATTTCAAGAATTGCTTAGCC+AGG	0.480187	3.2:+72563198	MS.gene77525:CDS
TTCGGTTATTGTCATCCAAT+GGG	0.481883	3.2:+72563232	MS.gene77525:CDS
AGGCTCATTCAAGAATGATA+CGG	0.500633	3.2:-72563173	None:intergenic
ATTCGGTTATTGTCATCCAA+TGG	0.511785	3.2:+72563231	MS.gene77525:CDS
GGCTCATTCAAGAATGATAC+GGG	0.516217	3.2:-72563172	None:intergenic
AACCGAATTCCTCTTCAGCC+TGG	0.542183	3.2:-72563216	None:intergenic
GGCCATCTTGCAGTGTATGT+TGG	0.549018	3.2:+72563124	MS.gene77525:CDS
ATTGCTTAGCCAGGCTGAAG+AGG	0.556864	3.2:+72563207	MS.gene77525:CDS
AAACAACTGGAAGTACCAAA+AGG	0.567081	3.2:+72563103	MS.gene77525:CDS
AAGAACATGTCTTCCTTGCA+GGG	0.575971	3.2:-72563268	None:intergenic
TGGTCTTACAATTCCCTGCA+AGG	0.603321	3.2:+72563255	MS.gene77525:CDS
CTCCAACATACACTGCAAGA+TGG	0.603726	3.2:-72563126	None:intergenic
GGAATTGTAAGACCACCCAT+TGG	0.609754	3.2:-72563247	None:intergenic
GATGACAACCTTCTAATACC+AGG	0.628886	3.2:-72563058	None:intergenic
GGTTATTGTCATCCAATGGG+TGG	0.630953	3.2:+72563235	MS.gene77525:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CTAAGCAATTCTTGAAATAA+AGG	-	chr3.2:72563196-72563215	None:intergenic	25.0%
	AGGCTCATTCAAGAATGATA+CGG	-	chr3.2:72563176-72563195	None:intergenic	35.0%
	ATACAACTTCTGTCTTGAAC+AGG	+	chr3.2:72563293-72563312	MS.gene77525:CDS	35.0%
	ATTCGGTTATTGTCATCCAA+TGG	+	chr3.2:72563231-72563250	MS.gene77525:CDS	35.0%
	ATTTCAAGAATTGCTTAGCC+AGG	+	chr3.2:72563198-72563217	MS.gene77525:CDS	35.0%
	TCAATCATCTAGCAAACAAC+TGG	+	chr3.2:72563090-72563109	MS.gene77525:CDS	35.0%
	TTCGGTTATTGTCATCCAAT+GGG	+	chr3.2:72563232-72563251	MS.gene77525:CDS	35.0%
!!	AAACAACTGGAAGTACCAAA+AGG	+	chr3.2:72563103-72563122	MS.gene77525:CDS	35.0%
	AAGAACATGTCTTCCTTGCA+GGG	-	chr3.2:72563271-72563290	None:intergenic	40.0%
	GATGACAACCTTCTAATACC+AGG	-	chr3.2:72563061-72563080	None:intergenic	40.0%
	GGCTCATTCAAGAATGATAC+GGG	-	chr3.2:72563175-72563194	None:intergenic	40.0%
	TAAGAACATGTCTTCCTTGC+AGG	-	chr3.2:72563272-72563291	None:intergenic	40.0%
	TTCGCTTACCTGGTATTAGA+AGG	+	chr3.2:72563050-72563069	MS.gene77525:CDS	40.0%
	CTCCAACATACACTGCAAGA+TGG	-	chr3.2:72563129-72563148	None:intergenic	45.0%
	GGAATTGTAAGACCACCCAT+TGG	-	chr3.2:72563250-72563269	None:intergenic	45.0%
	GGTTATTGTCATCCAATGGG+TGG	+	chr3.2:72563235-72563254	MS.gene77525:CDS	45.0%
!	TACACTGCAAGATGGCCTTT+TGG	-	chr3.2:72563121-72563140	None:intergenic	45.0%
!	TGGTCTTACAATTCCCTGCA+AGG	+	chr3.2:72563255-72563274	MS.gene77525:CDS	45.0%
	AACCGAATTCCTCTTCAGCC+TGG	-	chr3.2:72563219-72563238	None:intergenic	50.0%
	AGCCAGGCTGAAGAGGAATT+CGG	+	chr3.2:72563214-72563233	MS.gene77525:CDS	50.0%
	ATTGCTTAGCCAGGCTGAAG+AGG	+	chr3.2:72563207-72563226	MS.gene77525:CDS	50.0%
	GGCCATCTTGCAGTGTATGT+TGG	+	chr3.2:72563124-72563143	MS.gene77525:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	72563043	72563321	72563043	ID=MS.gene77525
chr3.2	mRNA	72563043	72563321	72563043	ID=MS.gene77525.t1;Parent=MS.gene77525
chr3.2	exon	72563043	72563321	72563043	ID=MS.gene77525.t1.exon1;Parent=MS.gene77525.t1
chr3.2	CDS	72563043	72563321	72563043	ID=cds.MS.gene77525.t1;Parent=MS.gene77525.t1

Gene Sequence	>MS.gene77525 ATGGGTTTTCGCTTACCTGGTATTAGAAGGTTGTCATCTAGTGCAAGTCAATCATCTAGCAAACAACTGGAAGTACCAAAAGGCCATCTTGCAGTGTATGTTGGAGAGAAAATGAGAAGATTCATGATTCCCGTATCATTCTTGAATGAGCCTTTATTTCAAGAATTGCTTAGCCAGGCTGAAGAGGAATTCGGTTATTGTCATCCAATGGGTGGTCTTACAATTCCCTGCAAGGAAGACATGTTCTTACATACAACTTCTGTCTTGAACAGGCTATAA
Protein sequence	>MS.gene77525.t1 MGFRLPGIRRLSSSASQSSSKQLEVPKGHLAVYVGEKMRRFMIPVSFLNEPLFQELLSQAEEEFGYCHPMGGLTIPCKEDMFLHTTSVLNRL