AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96284


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96284.t1 MTR_4g133070 88.785 214 10 4 4 216 1 201 4.51e-109 311
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96284.t1 AT5G20130 51.807 166 74 3 52 216 42 202 3.70e-41 139
MS.gene96284.t1 AT5G20130 51.807 166 74 3 52 216 71 231 1.79e-40 138

Find 57 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGGTTTGGGTGGGGTAGA+TGG 0.204366 8.4:+1355571 MS.gene96284:CDS
CTGGAGGAGGTTGGTTTGGG+TGG 0.219431 8.4:+1355562 MS.gene96284:CDS
AAGAGAGCGTTGTAAGAAAA+TGG 0.252399 8.4:+1355971 MS.gene96284:CDS
TCCATTGCTGTATGCACTTC+GGG 0.261348 8.4:+1355831 MS.gene96284:CDS
GTGCTGGAGGAGGTTGGTTT+GGG 0.308879 8.4:+1355559 MS.gene96284:CDS
GGTGCTGGAGGAGGTTGGTT+TGG 0.312842 8.4:+1355558 MS.gene96284:CDS
TGGAGGAGGTTGGTTTGGGT+GGG 0.343284 8.4:+1355563 MS.gene96284:CDS
GGAGGAGGTTGGTTTGGGTG+GGG 0.346451 8.4:+1355564 MS.gene96284:CDS
TTTGGGTGGGGTAGATGGTT+TGG 0.362531 8.4:+1355576 MS.gene96284:CDS
GCTGCTTAGTCGTCGTCAAT+TGG 0.383421 8.4:-1354600 None:intergenic
TTCAAAGAGCCATCAAAATC+AGG 0.393603 8.4:-1355919 None:intergenic
GGAGGTGACAGCGGCGGTGC+TGG 0.396880 8.4:+1355543 MS.gene96284:CDS
AGAGAGCGTTGTAAGAAAAT+GGG 0.401097 8.4:+1355972 MS.gene96284:CDS
CGGGGAGTGAGAAATGGATT+TGG 0.413388 8.4:+1355850 MS.gene96284:CDS
AATCAAAAGACGAGAAGAGC+TGG 0.418830 8.4:+1355515 MS.gene96284:CDS
AACGATGGCGTGACCTGATT+AGG 0.420437 8.4:-1354576 None:intergenic
CAAGCTAGTCTTACTATACT+TGG 0.438781 8.4:+1355636 MS.gene96284:CDS
TCCCGTATTCAATCCTAATC+AGG 0.439070 8.4:+1354563 MS.gene96284:CDS
CGAGAAGAGCTGGGAGGCGG+AGG 0.440547 8.4:+1355525 MS.gene96284:CDS
CTGCTTAGTCGTCGTCAATT+GGG 0.460767 8.4:-1354599 None:intergenic
GACAGCGGCGGTGCTGGAGG+AGG 0.464114 8.4:+1355549 MS.gene96284:CDS
GCACTTCGGGGAGTGAGAAA+TGG 0.464500 8.4:+1355844 MS.gene96284:CDS
GGTGGGGTAGATGGTTTGGC+TGG 0.477513 8.4:+1355580 MS.gene96284:CDS
AGAAAATTGAATTTGACAAG+TGG 0.484614 8.4:+1355484 MS.gene96284:CDS
ATCCATTGCTGTATGCACTT+CGG 0.485612 8.4:+1355830 MS.gene96284:CDS
CAGTATATCCTAGTTGCAAA+AGG 0.489427 8.4:+1355781 MS.gene96284:intron
TTGAATACGGGACTCCTCCT+AGG 0.496159 8.4:-1354552 None:intergenic
GACCTGATTAGGATTGAATA+CGG 0.498106 8.4:-1354565 None:intergenic
GGTGACAGCGGCGGTGCTGG+AGG 0.514884 8.4:+1355546 MS.gene96284:CDS
GAAAATTGAATTTGACAAGT+GGG 0.514902 8.4:+1355485 MS.gene96284:CDS
AGACGAGAAGAGCTGGGAGG+CGG 0.517079 8.4:+1355522 MS.gene96284:CDS
GCGGCGGTGCTGGAGGAGGT+TGG 0.517578 8.4:+1355553 MS.gene96284:CDS
AGAGGCAGGTGAATCTGCTT+CGG 0.520608 8.4:-1354681 None:intergenic
GCATTGGAAGGTGCATTAAG+TGG 0.525308 8.4:+1355459 MS.gene96284:CDS
GGATCAAGCTAGAAAAGCAT+TGG 0.529686 8.4:+1355443 MS.gene96284:intron
GATGGCTCTTTGAAGAAGGA+AGG 0.548351 8.4:+1355928 MS.gene96284:CDS
GTTGTTGTCTGAGAAGAGGC+AGG 0.558606 8.4:-1354695 None:intergenic
GAGAGCGTTGTAAGAAAATG+GGG 0.569827 8.4:+1355973 MS.gene96284:CDS
CTGGGAGGCGGAGGTGACAG+CGG 0.571119 8.4:+1355534 MS.gene96284:CDS
GGAGGCGGAGGTGACAGCGG+CGG 0.580483 8.4:+1355537 MS.gene96284:CDS
AAGTGGAGGAATGGAAAGCA+TGG 0.580913 8.4:+1354516 None:intergenic
ATCAAAAGACGAGAAGAGCT+GGG 0.583730 8.4:+1355516 MS.gene96284:CDS
CCCCGAAGTGCATACAGCAA+TGG 0.590470 8.4:-1355832 None:intergenic
GCAGGTGAATCTGCTTCGGT+TGG 0.595607 8.4:-1354677 None:intergenic
AACTGAGTTTGTGCTGTAAG+CGG 0.600011 8.4:-1354651 None:intergenic
CATTGGAAGGTGCATTAAGT+GGG 0.609653 8.4:+1355460 MS.gene96284:CDS
ATGGGAGCAATTGTAAACCT+AGG 0.620327 8.4:+1354535 MS.gene96284:CDS
ACCTGATTAGGATTGAATAC+GGG 0.620878 8.4:-1354564 None:intergenic
CAAGCTAGAAAAGCATTGGA+AGG 0.624701 8.4:+1355447 MS.gene96284:CDS
AGTGGAGGAATGGAAAGCAT+GGG 0.629305 8.4:+1354517 None:intergenic
TCGTCGTCAATTGGGAACGA+TGG 0.631589 8.4:-1354591 None:intergenic
ATACAGCAATGGATTGATGA+TGG 0.639169 8.4:-1355821 None:intergenic
CTCAGACAACAACAAAAGAG+AGG 0.641852 8.4:+1354705 MS.gene96284:CDS
GGAGCAATTGTAAACCTAGG+AGG 0.664395 8.4:+1354538 MS.gene96284:CDS
CCATTGCTGTATGCACTTCG+GGG 0.665878 8.4:+1355832 MS.gene96284:CDS
ATGGATTGATGATGGCAGCG+AGG 0.723355 8.4:-1355813 None:intergenic
AAAAGACGAGAAGAGCTGGG+AGG 0.769117 8.4:+1355519 MS.gene96284:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCCATATATTTATTATT+AGG - chr8.4:1355039-1355058 None:intergenic 10.0%
!!! TTTATCTTAATTTATTTTGT+TGG + chr8.4:1355138-1355157 MS.gene96284:intron 10.0%
!! AAGTTGTATCTTATTATATT+CGG - chr8.4:1354981-1355000 None:intergenic 15.0%
!! AATTAAATATGTTCTACATT+AGG - chr8.4:1355167-1355186 None:intergenic 15.0%
!! ATTGGAATCATAAATAAATT+AGG - chr8.4:1355016-1355035 None:intergenic 15.0%
!! CAATCCTAATAATAAATATA+TGG + chr8.4:1355032-1355051 MS.gene96284:intron 15.0%
!! CATATATTTATTATTAGGAT+TGG - chr8.4:1355034-1355053 None:intergenic 15.0%
!! ACAAAAAATAAAACTGAAGT+TGG - chr8.4:1354792-1354811 None:intergenic 20.0%
!! ATATGCTATTATAAATAGAG+AGG - chr8.4:1354954-1354973 None:intergenic 20.0%
!! CAAAAAATAAAACTGAAGTT+GGG - chr8.4:1354791-1354810 None:intergenic 20.0%
!! CAGTGATAAATTAATTTCTA+TGG - chr8.4:1355292-1355311 None:intergenic 20.0%
!! TGAATGAATGAATATGAAAT+AGG - chr8.4:1354737-1354756 None:intergenic 20.0%
! AGAAAATTGAATTTGACAAG+TGG + chr8.4:1355484-1355503 MS.gene96284:CDS 25.0%
! GAAAATTGAATTTGACAAGT+GGG + chr8.4:1355485-1355504 MS.gene96284:CDS 25.0%
! GTCTTGAAAAATACTTCTAA+CGG + chr8.4:1355889-1355908 MS.gene96284:CDS 25.0%
! TAAAGAAATGAATTGCTATG+AGG - chr8.4:1354882-1354901 None:intergenic 25.0%
! TAAATATGTTCTACATTAGG+AGG - chr8.4:1355164-1355183 None:intergenic 25.0%
!! AATTTTGTAGTAGCATTTCA+TGG + chr8.4:1354846-1354865 MS.gene96284:intron 25.0%
!! ATAGTCTCTGATCTAATTTT+GGG + chr8.4:1355251-1355270 MS.gene96284:intron 25.0%
!! GGTCTAATGATGATAATTTT+TGG + chr8.4:1355601-1355620 MS.gene96284:CDS 25.0%
!! TATAGTCTCTGATCTAATTT+TGG + chr8.4:1355250-1355269 MS.gene96284:intron 25.0%
AATCAACATAGTATGAGAGT+AGG - chr8.4:1354918-1354937 None:intergenic 30.0%
ACTGCAAATAAAGAGAGATA+AGG - chr8.4:1355765-1355784 None:intergenic 30.0%
ATGCTACTACAAAATTATGC+AGG - chr8.4:1354842-1354861 None:intergenic 30.0%
ATTTATAGTCTCAGATATGG+GGG + chr8.4:1355345-1355364 MS.gene96284:intron 30.0%
CAAACAAGTTCAGTTAAGAA+AGG - chr8.4:1355110-1355129 None:intergenic 30.0%
CATTTATAGTCTCAGATATG+GGG + chr8.4:1355344-1355363 MS.gene96284:intron 30.0%
GCATTTATAGTCTCAGATAT+GGG + chr8.4:1355343-1355362 MS.gene96284:intron 30.0%
TATAAGTGAATTCTGATTGC+AGG + chr8.4:1355422-1355441 MS.gene96284:intron 30.0%
! CATATCTGAGACTATAAATG+CGG - chr8.4:1355344-1355363 None:intergenic 30.0%
! CTTTCTTAACTGAACTTGTT+TGG + chr8.4:1355108-1355127 MS.gene96284:intron 30.0%
! TAGTCTCTGATCTAATTTTG+GGG + chr8.4:1355252-1355271 MS.gene96284:intron 30.0%
AAAATTATGCAGGCAGTGAA+AGG - chr8.4:1354832-1354851 None:intergenic 35.0%
AATTTGAGAAGTGAGTTCTG+AGG + chr8.4:1355389-1355408 MS.gene96284:intron 35.0%
ACCTGATTAGGATTGAATAC+GGG - chr8.4:1354567-1354586 None:intergenic 35.0%
CAGTATATCCTAGTTGCAAA+AGG + chr8.4:1355781-1355800 MS.gene96284:intron 35.0%
CGCATTTATAGTCTCAGATA+TGG + chr8.4:1355342-1355361 MS.gene96284:intron 35.0%
GACCTGATTAGGATTGAATA+CGG - chr8.4:1354568-1354587 None:intergenic 35.0%
TGCAATTTACTGTCACACTT+TGG + chr8.4:1355078-1355097 MS.gene96284:intron 35.0%
TTCAAAGAGCCATCAAAATC+AGG - chr8.4:1355922-1355941 None:intergenic 35.0%
! ACTCTTCCGTTGATGAAAAA+TGG + chr8.4:1354626-1354645 MS.gene96284:CDS 35.0%
! ATTTGAGAAGTGAGTTCTGA+GGG + chr8.4:1355390-1355409 MS.gene96284:intron 35.0%
! TCAAATCACCTTTTGCAACT+AGG - chr8.4:1355792-1355811 None:intergenic 35.0%
!! AAGAGAGCGTTGTAAGAAAA+TGG + chr8.4:1355971-1355990 MS.gene96284:CDS 35.0%
!! AGAGAGCGTTGTAAGAAAAT+GGG + chr8.4:1355972-1355991 MS.gene96284:CDS 35.0%
!! ATACAGCAATGGATTGATGA+TGG - chr8.4:1355824-1355843 None:intergenic 35.0%
!! CAAGCTAGTCTTACTATACT+TGG + chr8.4:1355636-1355655 MS.gene96284:CDS 35.0%
!! TTTTGATGGCTCTTTGAAGA+AGG + chr8.4:1355924-1355943 MS.gene96284:CDS 35.0%
!!! TTTTGTTGTTGTCTGAGAAG+AGG - chr8.4:1354702-1354721 None:intergenic 35.0%
AACTGAGTTTGTGCTGTAAG+CGG - chr8.4:1354654-1354673 None:intergenic 40.0%
AATCAAAAGACGAGAAGAGC+TGG + chr8.4:1355515-1355534 MS.gene96284:CDS 40.0%
ATCAAAAGACGAGAAGAGCT+GGG + chr8.4:1355516-1355535 MS.gene96284:CDS 40.0%
ATCCATTGCTGTATGCACTT+CGG + chr8.4:1355830-1355849 MS.gene96284:CDS 40.0%
ATGGGAGCAATTGTAAACCT+AGG + chr8.4:1354535-1354554 MS.gene96284:CDS 40.0%
CAGATATGGGGGAATTAAGT+GGG + chr8.4:1355356-1355375 MS.gene96284:intron 40.0%
CTCAGACAACAACAAAAGAG+AGG + chr8.4:1354705-1354724 MS.gene96284:CDS 40.0%
GACAACAACAAAAGAGAGGT+AGG + chr8.4:1354709-1354728 MS.gene96284:intron 40.0%
TCAGATATGGGGGAATTAAG+TGG + chr8.4:1355355-1355374 MS.gene96284:intron 40.0%
TCCCGTATTCAATCCTAATC+AGG + chr8.4:1354563-1354582 MS.gene96284:CDS 40.0%
! CATTGGAAGGTGCATTAAGT+GGG + chr8.4:1355460-1355479 MS.gene96284:CDS 40.0%
! GGATCAAGCTAGAAAAGCAT+TGG + chr8.4:1355443-1355462 MS.gene96284:intron 40.0%
!! CAAGCTAGAAAAGCATTGGA+AGG + chr8.4:1355447-1355466 MS.gene96284:CDS 40.0%
!! GAGAGCGTTGTAAGAAAATG+GGG + chr8.4:1355973-1355992 MS.gene96284:CDS 40.0%
CTGCTTAGTCGTCGTCAATT+GGG - chr8.4:1354602-1354621 None:intergenic 45.0%
GGAGCAATTGTAAACCTAGG+AGG + chr8.4:1354538-1354557 MS.gene96284:CDS 45.0%
TCCATTGCTGTATGCACTTC+GGG + chr8.4:1355831-1355850 MS.gene96284:CDS 45.0%
! GATGGCTCTTTGAAGAAGGA+AGG + chr8.4:1355928-1355947 MS.gene96284:CDS 45.0%
! GCATTGGAAGGTGCATTAAG+TGG + chr8.4:1355459-1355478 MS.gene96284:CDS 45.0%
! GGTGATCAGCCTGATTTTGA+TGG + chr8.4:1355910-1355929 MS.gene96284:CDS 45.0%
!! GCGGCTCCATTTTTCATCAA+CGG - chr8.4:1354635-1354654 None:intergenic 45.0%
AAAAGACGAGAAGAGCTGGG+AGG + chr8.4:1355519-1355538 MS.gene96284:CDS 50.0%
AACGATGGCGTGACCTGATT+AGG - chr8.4:1354579-1354598 None:intergenic 50.0%
AGAGGCAGGTGAATCTGCTT+CGG - chr8.4:1354684-1354703 None:intergenic 50.0%
CCATTGCTGTATGCACTTCG+GGG + chr8.4:1355832-1355851 MS.gene96284:CDS 50.0%
GCTGCTTAGTCGTCGTCAAT+TGG - chr8.4:1354603-1354622 None:intergenic 50.0%
GTTGTTGTCTGAGAAGAGGC+AGG - chr8.4:1354698-1354717 None:intergenic 50.0%
TCGTCGTCAATTGGGAACGA+TGG - chr8.4:1354594-1354613 None:intergenic 50.0%
TTGAATACGGGACTCCTCCT+AGG - chr8.4:1354555-1354574 None:intergenic 50.0%
TTTGGGTGGGGTAGATGGTT+TGG + chr8.4:1355576-1355595 MS.gene96284:CDS 50.0%
! CGGGGAGTGAGAAATGGATT+TGG + chr8.4:1355850-1355869 MS.gene96284:CDS 50.0%
!! ATGGATTGATGATGGCAGCG+AGG - chr8.4:1355816-1355835 None:intergenic 50.0%
CCCCGAAGTGCATACAGCAA+TGG - chr8.4:1355835-1355854 None:intergenic 55.0%
GCACTTCGGGGAGTGAGAAA+TGG + chr8.4:1355844-1355863 MS.gene96284:CDS 55.0%
GCAGGTGAATCTGCTTCGGT+TGG - chr8.4:1354680-1354699 None:intergenic 55.0%
!! GTGCTGGAGGAGGTTGGTTT+GGG + chr8.4:1355559-1355578 MS.gene96284:CDS 55.0%
!! GTTGGTTTGGGTGGGGTAGA+TGG + chr8.4:1355571-1355590 MS.gene96284:CDS 55.0%
!! TGGAGGAGGTTGGTTTGGGT+GGG + chr8.4:1355563-1355582 MS.gene96284:CDS 55.0%
AGACGAGAAGAGCTGGGAGG+CGG + chr8.4:1355522-1355541 MS.gene96284:CDS 60.0%
! GGTGCTGGAGGAGGTTGGTT+TGG + chr8.4:1355558-1355577 MS.gene96284:CDS 60.0%
! GGTGGGGTAGATGGTTTGGC+TGG + chr8.4:1355580-1355599 MS.gene96284:CDS 60.0%
!! CTGGAGGAGGTTGGTTTGGG+TGG + chr8.4:1355562-1355581 MS.gene96284:CDS 60.0%
!! GGAGGAGGTTGGTTTGGGTG+GGG + chr8.4:1355564-1355583 MS.gene96284:CDS 60.0%
CGAGAAGAGCTGGGAGGCGG+AGG + chr8.4:1355525-1355544 MS.gene96284:CDS 70.0%
CTGGGAGGCGGAGGTGACAG+CGG + chr8.4:1355534-1355553 MS.gene96284:CDS 70.0%
GGAGGCGGAGGTGACAGCGG+CGG + chr8.4:1355537-1355556 MS.gene96284:CDS 75.0%
GGAGGTGACAGCGGCGGTGC+TGG + chr8.4:1355543-1355562 MS.gene96284:CDS 75.0%
! GACAGCGGCGGTGCTGGAGG+AGG + chr8.4:1355549-1355568 MS.gene96284:CDS 75.0%
! GCGGCGGTGCTGGAGGAGGT+TGG + chr8.4:1355553-1355572 MS.gene96284:CDS 75.0%
! GGTGACAGCGGCGGTGCTGG+AGG + chr8.4:1355546-1355565 MS.gene96284:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 1354526 1356005 1354526 ID=MS.gene96284
chr8.4 mRNA 1354526 1356005 1354526 ID=MS.gene96284.t1;Parent=MS.gene96284
chr8.4 exon 1354526 1354726 1354526 ID=MS.gene96284.t1.exon1;Parent=MS.gene96284.t1
chr8.4 CDS 1354526 1354726 1354526 ID=cds.MS.gene96284.t1;Parent=MS.gene96284.t1
chr8.4 exon 1355444 1355671 1355444 ID=MS.gene96284.t1.exon2;Parent=MS.gene96284.t1
chr8.4 CDS 1355444 1355671 1355444 ID=cds.MS.gene96284.t1;Parent=MS.gene96284.t1
chr8.4 exon 1355784 1356005 1355784 ID=MS.gene96284.t1.exon3;Parent=MS.gene96284.t1
chr8.4 CDS 1355784 1356005 1355784 ID=cds.MS.gene96284.t1;Parent=MS.gene96284.t1
Gene Sequence

>MS.gene96284

ATGGAAAGCATGGGAGCAATTGTAAACCTAGGAGGAGTCCCGTATTCAATCCTAATCAGGTCACGCCATCGTTCCCAATTGACGACGACTAAGCAGCTTGACTCTTCCGTTGATGAAAAATGGAGCCGCTTACAGCACAAACTCAGTTTCACCAACCGAAGCAGATTCACCTGCCTCTTCTCAGACAACAACAAAAGAGAGGTAGGTACCTATTTCATATTCATTCATTCATTCATTCGCATATATCATATCAATTTAATAACCCAACTTCAGTTTTATTTTTTGTTGCTACTAATATTCAATCCTTTCACTGCCTGCATAATTTTGTAGTAGCATTTCATGGATTGTTTGTTCCTCATAGCAATTCATTTCTTTATGATTAGAACGAACCTACTCTCATACTATGTTGATTTTGTGATTTCTGACCTCTCTATTTATAATAGCATATAGAACCGAATATAATAAGATACAACTTTCTAATTTAAATCCTAATTTATTTATGATTCCAATCCTAATAATAAATATATGGATTTCATTCTAACACTTTCTACATGCAATTTACTGTCACACTTTGGAAAATACCTTTCTTAACTGAACTTGTTTGGTTTGTTATTTATCTTAATTTATTTTGTTGGCCTCCTAATGTAGAACATATTTAATTTTCATGATACTAGCAAAATTGTATCATGCACTTTAGTTTGCAAAAGACAAAAATAATTCTCAATATAGTCTCTGATCTAATTTTGGGGTCATGATCAGTTATCCATAGAAATTAATTTATCACTGAAATATATTTCATATCTTGTGATGATGAGCCGCATTTATAGTCTCAGATATGGGGGAATTAAGTGGGAAGAAAATTGAATTTGAGAAGTGAGTTCTGAGGGAAATGAAACTATAAGTGAATTCTGATTGCAGGATCAAGCTAGAAAAGCATTGGAAGGTGCATTAAGTGGGAAGAAAATTGAATTTGACAAGTGGGACAAAGAAATCAAAAGACGAGAAGAGCTGGGAGGCGGAGGTGACAGCGGCGGTGCTGGAGGAGGTTGGTTTGGGTGGGGTAGATGGTTTGGCTGGTCTAATGATGATAATTTTTGGCAAGAAGCAAAACAAGCTAGTCTTACTATACTTGGCATCGTTCTCATAGTAAGAAATTTTCTCACACTACTTTCAATTCTTTTGCTGCAAGTTCAAAACTAATTAATATTATTATTATAAAATAAAAAAACTATTTTACCTTATCTCTCTTTATTTGCAGTATATCCTAGTTGCAAAAGGTGATTTGATCCTCGCTGCCATCATCAATCCATTGCTGTATGCACTTCGGGGAGTGAGAAATGGATTTGGTCTCGCAACATCACAAGTCTTGAAAAATACTTCTAACGGTGATCAGCCTGATTTTGATGGCTCTTTGAAGAAGGAAGGCTACAAAAGTGCTTCTGCTAAAGAGAGCGTTGTAAGAAAATGGGGTAGTGATTAG

Protein sequence

>MS.gene96284.t1

MESMGAIVNLGGVPYSILIRSRHRSQLTTTKQLDSSVDEKWSRLQHKLSFTNRSRFTCLFSDNNKREDQARKALEGALSGKKIEFDKWDKEIKRREELGGGGDSGGAGGGWFGWGRWFGWSNDDNFWQEAKQASLTILGIVLIYILVAKGDLILAAIINPLLYALRGVRNGFGLATSQVLKNTSNGDQPDFDGSLKKEGYKSASAKESVVRKWGSD