Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96680.t1 | XP_013452267.1 | 95.2 | 124 | 6 | 0 | 1 | 124 | 46 | 169 | 2.70E-58 | 234.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96680.t1 | Q9LTD9 | 42.1 | 121 | 62 | 1 | 3 | 123 | 45 | 157 | 1.8e-18 | 93.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96680.t1 | A0A072UKD7 | 95.2 | 124 | 6 | 0 | 1 | 124 | 46 | 169 | 2.0e-58 | 234.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene96680 | 0.808526 | 2.97E-50 | -1.69E-46 |
| MS.gene049346 | MS.gene96680 | 0.819934 | 8.95E-53 | -1.69E-46 |
| MS.gene049661 | MS.gene96680 | 0.828497 | 8.70E-55 | -1.69E-46 |
| MS.gene049662 | MS.gene96680 | 0.827413 | 1.59E-54 | -1.69E-46 |
| MS.gene049758 | MS.gene96680 | 0.818782 | 1.64E-52 | -1.69E-46 |
| MS.gene049910 | MS.gene96680 | 0.801112 | 1.05E-48 | -1.69E-46 |
| MS.gene050076 | MS.gene96680 | 0.865029 | 7.94E-65 | -1.69E-46 |
| MS.gene050301 | MS.gene96680 | 0.823263 | 1.52E-53 | -1.69E-46 |
| MS.gene050397 | MS.gene96680 | 0.862155 | 6.19E-64 | -1.69E-46 |
| MS.gene050404 | MS.gene96680 | 0.889524 | 2.24E-73 | -1.69E-46 |
| MS.gene050406 | MS.gene96680 | 0.913589 | 5.16E-84 | -1.69E-46 |
| MS.gene050426 | MS.gene96680 | -0.823018 | 1.74E-53 | -1.69E-46 |
| MS.gene050432 | MS.gene96680 | 0.81584 | 7.52E-52 | -1.69E-46 |
| MS.gene050464 | MS.gene96680 | 0.862641 | 4.39E-64 | -1.69E-46 |
| MS.gene050529 | MS.gene96680 | 0.88737 | 1.51E-72 | -1.69E-46 |
| MS.gene050642 | MS.gene96680 | 0.804301 | 2.31E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene96680 | 0.828078 | 1.10E-54 | -1.69E-46 |
| MS.gene050812 | MS.gene96680 | 0.831319 | 1.79E-55 | -1.69E-46 |
| MS.gene05101 | MS.gene96680 | 0.835802 | 1.36E-56 | -1.69E-46 |
| MS.gene05105 | MS.gene96680 | 0.824809 | 6.60E-54 | -1.69E-46 |
| MS.gene051171 | MS.gene96680 | 0.846935 | 1.59E-59 | -1.69E-46 |
| MS.gene051242 | MS.gene96680 | 0.82569 | 4.08E-54 | -1.69E-46 |
| MS.gene051244 | MS.gene96680 | 0.8026 | 5.19E-49 | -1.69E-46 |
| MS.gene051471 | MS.gene96680 | 0.863829 | 1.88E-64 | -1.69E-46 |
| MS.gene051582 | MS.gene96680 | 0.860712 | 1.71E-63 | -1.69E-46 |
| MS.gene052077 | MS.gene96680 | 0.836699 | 8.03E-57 | -1.69E-46 |
| MS.gene052078 | MS.gene96680 | 0.870382 | 1.52E-66 | -1.69E-46 |
| MS.gene052178 | MS.gene96680 | 0.829595 | 4.71E-55 | -1.69E-46 |
| MS.gene052188 | MS.gene96680 | 0.824927 | 6.19E-54 | -1.69E-46 |
| MS.gene052253 | MS.gene96680 | 0.826889 | 2.12E-54 | -1.69E-46 |
| MS.gene052290 | MS.gene96680 | 0.827094 | 1.89E-54 | -1.69E-46 |
| MS.gene052310 | MS.gene96680 | 0.885016 | 1.17E-71 | -1.69E-46 |
| MS.gene052322 | MS.gene96680 | 0.827538 | 1.48E-54 | -1.69E-46 |
| MS.gene052326 | MS.gene96680 | 0.808945 | 2.41E-50 | -1.69E-46 |
| MS.gene052340 | MS.gene96680 | 0.858577 | 7.49E-63 | -1.69E-46 |
| MS.gene052481 | MS.gene96680 | 0.801257 | 9.81E-49 | -1.69E-46 |
| MS.gene052758 | MS.gene96680 | 0.8182 | 2.22E-52 | -1.69E-46 |
| MS.gene053103 | MS.gene96680 | 0.841158 | 5.63E-58 | -1.69E-46 |
| MS.gene053209 | MS.gene96680 | 0.814795 | 1.28E-51 | -1.69E-46 |
| MS.gene053330 | MS.gene96680 | 0.835095 | 2.05E-56 | -1.69E-46 |
| MS.gene053557 | MS.gene96680 | 0.840069 | 1.09E-57 | -1.69E-46 |
| MS.gene053836 | MS.gene96680 | 0.851989 | 6.19E-61 | -1.69E-46 |
| MS.gene053907 | MS.gene96680 | 0.846535 | 2.04E-59 | -1.69E-46 |
| MS.gene053928 | MS.gene96680 | 0.811803 | 5.83E-51 | -1.69E-46 |
| MS.gene053971 | MS.gene96680 | 0.89139 | 4.15E-74 | -1.69E-46 |
| MS.gene054123 | MS.gene96680 | 0.812637 | 3.83E-51 | -1.69E-46 |
| MS.gene054349 | MS.gene96680 | 0.810457 | 1.14E-50 | -1.69E-46 |
| MS.gene054802 | MS.gene96680 | 0.803757 | 2.99E-49 | -1.69E-46 |
| MS.gene054803 | MS.gene96680 | 0.801692 | 7.99E-49 | -1.69E-46 |
| MS.gene054804 | MS.gene96680 | 0.804538 | 2.06E-49 | -1.69E-46 |
| MS.gene054918 | MS.gene96680 | 0.809835 | 1.55E-50 | -1.69E-46 |
| MS.gene054983 | MS.gene96680 | 0.823518 | 1.33E-53 | -1.69E-46 |
| MS.gene055116 | MS.gene96680 | 0.855581 | 5.73E-62 | -1.69E-46 |
| MS.gene055254 | MS.gene96680 | 0.808593 | 2.87E-50 | -1.69E-46 |
| MS.gene055277 | MS.gene96680 | 0.850996 | 1.18E-60 | -1.69E-46 |
| MS.gene055370 | MS.gene96680 | 0.858248 | 9.39E-63 | -1.69E-46 |
| MS.gene055422 | MS.gene96680 | 0.828454 | 8.91E-55 | -1.69E-46 |
| MS.gene055428 | MS.gene96680 | 0.870875 | 1.05E-66 | -1.69E-46 |
| MS.gene05553 | MS.gene96680 | 0.85059 | 1.54E-60 | -1.69E-46 |
| MS.gene055601 | MS.gene96680 | -0.803717 | 3.05E-49 | -1.69E-46 |
| MS.gene055712 | MS.gene96680 | 0.800861 | 1.18E-48 | -1.69E-46 |
| MS.gene055980 | MS.gene96680 | 0.801689 | 8.00E-49 | -1.69E-46 |
| MS.gene056191 | MS.gene96680 | 0.801386 | 9.23E-49 | -1.69E-46 |
| MS.gene056407 | MS.gene96680 | 0.856331 | 3.46E-62 | -1.69E-46 |
| MS.gene056451 | MS.gene96680 | 0.859995 | 2.81E-63 | -1.69E-46 |
| MS.gene056609 | MS.gene96680 | 0.802452 | 5.57E-49 | -1.69E-46 |
| MS.gene056612 | MS.gene96680 | 0.867709 | 1.12E-65 | -1.69E-46 |
| MS.gene056633 | MS.gene96680 | 0.840237 | 9.82E-58 | -1.69E-46 |
| MS.gene056659 | MS.gene96680 | 0.818142 | 2.29E-52 | -1.69E-46 |
| MS.gene056664 | MS.gene96680 | 0.865616 | 5.19E-65 | -1.69E-46 |
| MS.gene056796 | MS.gene96680 | 0.817279 | 3.58E-52 | -1.69E-46 |
| MS.gene056899 | MS.gene96680 | -0.838807 | 2.31E-57 | -1.69E-46 |
| MS.gene05702 | MS.gene96680 | 0.898009 | 8.07E-77 | -1.69E-46 |
| MS.gene057123 | MS.gene96680 | 0.833404 | 5.44E-56 | -1.69E-46 |
| MS.gene057196 | MS.gene96680 | 0.811068 | 8.42E-51 | -1.69E-46 |
| MS.gene057295 | MS.gene96680 | 0.803526 | 3.34E-49 | -1.69E-46 |
| MS.gene05733 | MS.gene96680 | 0.811801 | 5.84E-51 | -1.69E-46 |
| MS.gene057439 | MS.gene96680 | 0.803488 | 3.40E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene96680 | 0.810739 | 9.92E-51 | -1.69E-46 |
| MS.gene057823 | MS.gene96680 | 0.806541 | 7.83E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene96680 | 0.845867 | 3.11E-59 | -1.69E-46 |
| MS.gene058082 | MS.gene96680 | 0.81731 | 3.52E-52 | -1.69E-46 |
| MS.gene05811 | MS.gene96680 | 0.805944 | 1.05E-49 | -1.69E-46 |
| MS.gene058142 | MS.gene96680 | 0.837173 | 6.08E-57 | -1.69E-46 |
| MS.gene058233 | MS.gene96680 | 0.895695 | 7.51E-76 | -1.69E-46 |
| MS.gene058276 | MS.gene96680 | 0.876142 | 1.77E-68 | -1.69E-46 |
| MS.gene058293 | MS.gene96680 | 0.823221 | 1.56E-53 | -1.69E-46 |
| MS.gene058365 | MS.gene96680 | 0.809359 | 1.97E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene96680 | 0.835648 | 1.48E-56 | -1.69E-46 |
| MS.gene058710 | MS.gene96680 | 0.809405 | 1.92E-50 | -1.69E-46 |
| MS.gene058746 | MS.gene96680 | 0.881687 | 1.95E-70 | -1.69E-46 |
| MS.gene058928 | MS.gene96680 | 0.802373 | 5.79E-49 | -1.69E-46 |
| MS.gene059011 | MS.gene96680 | 0.805733 | 1.16E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene96680 | 0.840866 | 6.72E-58 | -1.69E-46 |
| MS.gene059285 | MS.gene96680 | 0.823996 | 1.03E-53 | -1.69E-46 |
| MS.gene059326 | MS.gene96680 | 0.806495 | 8.00E-50 | -1.69E-46 |
| MS.gene059518 | MS.gene96680 | 0.816458 | 5.47E-52 | -1.69E-46 |
| MS.gene059597 | MS.gene96680 | 0.844168 | 8.94E-59 | -1.69E-46 |
| MS.gene059676 | MS.gene96680 | 0.81456 | 1.45E-51 | -1.69E-46 |
| MS.gene059688 | MS.gene96680 | 0.840188 | 1.01E-57 | -1.69E-46 |
| MS.gene059692 | MS.gene96680 | 0.855314 | 6.86E-62 | -1.69E-46 |
| MS.gene059695 | MS.gene96680 | 0.854776 | 9.83E-62 | -1.69E-46 |
| MS.gene05984 | MS.gene96680 | 0.820177 | 7.87E-53 | -1.69E-46 |
| MS.gene059894 | MS.gene96680 | 0.890883 | 6.59E-74 | -1.69E-46 |
| MS.gene059960 | MS.gene96680 | 0.80807 | 3.71E-50 | -1.69E-46 |
| MS.gene06037 | MS.gene96680 | 0.808168 | 3.54E-50 | -1.69E-46 |
| MS.gene060403 | MS.gene96680 | 0.834316 | 3.22E-56 | -1.69E-46 |
| MS.gene060407 | MS.gene96680 | 0.868995 | 4.31E-66 | -1.69E-46 |
| MS.gene06090 | MS.gene96680 | 0.812453 | 4.21E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene96680 | 0.893126 | 8.40E-75 | -1.69E-46 |
| MS.gene061112 | MS.gene96680 | 0.831551 | 1.57E-55 | -1.69E-46 |
| MS.gene061159 | MS.gene96680 | 0.815239 | 1.02E-51 | -1.69E-46 |
| MS.gene061265 | MS.gene96680 | 0.826795 | 2.23E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96680.t1 | MTR_6g051780 | 95.161 | 124 | 6 | 0 | 1 | 124 | 46 | 169 | 1.37e-69 | 207 |
| MS.gene96680.t1 | MTR_5g027010 | 42.529 | 87 | 50 | 0 | 38 | 124 | 100 | 186 | 1.96e-19 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96680.t1 | AT5G52780 | 48.810 | 84 | 43 | 0 | 40 | 123 | 74 | 157 | 3.27e-24 | 91.7 |
| MS.gene96680.t1 | AT4G19100 | 42.529 | 87 | 50 | 0 | 38 | 124 | 49 | 135 | 1.05e-18 | 77.0 |
| MS.gene96680.t1 | AT4G19100 | 42.529 | 87 | 50 | 0 | 38 | 124 | 112 | 198 | 1.14e-18 | 78.6 |
Find 29 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATCATTCCTGCATCTTTA+TGG | 0.158172 | 8.1:-27551897 | MS.gene96680:CDS |
| TTTGAAGAAGTGAGAAGAAT+TGG | 0.269974 | 8.1:-27551726 | MS.gene96680:CDS |
| ACTTTAGTTCCAATGGCATT+AGG | 0.278677 | 8.1:-27551921 | MS.gene96680:CDS |
| TCCTTAGCTTTATTTGTTGA+TGG | 0.314102 | 8.1:+27552041 | None:intergenic |
| CTTTAGTTCCAATGGCATTA+GGG | 0.325438 | 8.1:-27551920 | MS.gene96680:CDS |
| TGGTGTGCTTTCTTCTAGTT+TGG | 0.335101 | 8.1:-27551778 | MS.gene96680:CDS |
| ATCATCATCATTCTTATTGT+TGG | 0.338614 | 8.1:+27551995 | None:intergenic |
| ATTCTATACATAACTTCCTT+AGG | 0.373433 | 8.1:+27551966 | None:intergenic |
| TCATCATCATTCTTATTGTT+GGG | 0.386695 | 8.1:+27551996 | None:intergenic |
| TTGAAGAAGTGAGAAGAATT+GGG | 0.394639 | 8.1:-27551725 | MS.gene96680:CDS |
| GAAGTTATGTATAGAATAAT+CGG | 0.401140 | 8.1:-27551960 | MS.gene96680:CDS |
| GAGAGAGAAGGTTCTTTGTT+GGG | 0.409732 | 8.1:-27551750 | MS.gene96680:CDS |
| TCTGGACTTGGAATTGCATA+TGG | 0.435952 | 8.1:-27551798 | MS.gene96680:CDS |
| AAATATTTGATGCTCCTTTG+TGG | 0.443314 | 8.1:-27551857 | MS.gene96680:CDS |
| AGAGAGAGAAGGTTCTTTGT+TGG | 0.444549 | 8.1:-27551751 | MS.gene96680:CDS |
| TCTTGTGTTGGAGAAGCCTT+TGG | 0.450187 | 8.1:+27552067 | None:intergenic |
| AATACAAGAGTTGTCACAAA+TGG | 0.482315 | 8.1:+27551831 | None:intergenic |
| GTTTAGTACTTTAGTTCCAA+TGG | 0.487189 | 8.1:-27551928 | MS.gene96680:CDS |
| TTAGCTTTATTTGTTGATGG+TGG | 0.488414 | 8.1:+27552044 | None:intergenic |
| TGTTGATGGTGGTCTTGTGT+TGG | 0.494927 | 8.1:+27552055 | None:intergenic |
| TTAACTCACCATAAAGATGC+AGG | 0.497091 | 8.1:+27551889 | None:intergenic |
| AAGCCTTTGGCACTAGCTTG+TGG | 0.500076 | 8.1:+27552080 | None:intergenic |
| AATGATAACCCTAATGCCAT+TGG | 0.526208 | 8.1:+27551912 | None:intergenic |
| ACCATCAACAAATAAAGCTA+AGG | 0.559111 | 8.1:-27552042 | MS.gene96680:CDS |
| AGTTTGGATGCAGAGAGAGA+AGG | 0.579276 | 8.1:-27551762 | MS.gene96680:CDS |
| TGATGATGATGATATTCCTA+AGG | 0.584648 | 8.1:-27551982 | MS.gene96680:CDS |
| AAGCCACAAGCTAGTGCCAA+AGG | 0.610001 | 8.1:-27552083 | MS.gene96680:CDS |
| GTCACAAATGGCATCCACAA+AGG | 0.623023 | 8.1:+27551843 | None:intergenic |
| AGAAGAATTGGGTTGAGATG+TGG | 0.651644 | 8.1:-27551714 | MS.gene96680:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAGTTATGTATAGAATAAT+CGG | - | chr8.1:27551818-27551837 | MS.gene96680:CDS | 20.0% |
| ! | ATCATCATCATTCTTATTGT+TGG | + | chr8.1:27551786-27551805 | None:intergenic | 25.0% |
| ! | ATTCTATACATAACTTCCTT+AGG | + | chr8.1:27551815-27551834 | None:intergenic | 25.0% |
| ! | TCATCATCATTCTTATTGTT+GGG | + | chr8.1:27551785-27551804 | None:intergenic | 25.0% |
| AAATATTTGATGCTCCTTTG+TGG | - | chr8.1:27551921-27551940 | MS.gene96680:CDS | 30.0% | |
| AATACAAGAGTTGTCACAAA+TGG | + | chr8.1:27551950-27551969 | None:intergenic | 30.0% | |
| ACCATCAACAAATAAAGCTA+AGG | - | chr8.1:27551736-27551755 | MS.gene96680:CDS | 30.0% | |
| TTATCATTCCTGCATCTTTA+TGG | - | chr8.1:27551881-27551900 | MS.gene96680:CDS | 30.0% | |
| TTGAAGAAGTGAGAAGAATT+GGG | - | chr8.1:27552053-27552072 | MS.gene96680:CDS | 30.0% | |
| TTTGAAGAAGTGAGAAGAAT+TGG | - | chr8.1:27552052-27552071 | MS.gene96680:CDS | 30.0% | |
| ! | GTTTAGTACTTTAGTTCCAA+TGG | - | chr8.1:27551850-27551869 | MS.gene96680:CDS | 30.0% |
| ! | TGATGATGATGATATTCCTA+AGG | - | chr8.1:27551796-27551815 | MS.gene96680:CDS | 30.0% |
| !! | TCCTTAGCTTTATTTGTTGA+TGG | + | chr8.1:27551740-27551759 | None:intergenic | 30.0% |
| !! | TTAGCTTTATTTGTTGATGG+TGG | + | chr8.1:27551737-27551756 | None:intergenic | 30.0% |
| !!! | GTGACAACTCTTGTATTTTT+TGG | - | chr8.1:27551953-27551972 | MS.gene96680:CDS | 30.0% |
| AATGATAACCCTAATGCCAT+TGG | + | chr8.1:27551869-27551888 | None:intergenic | 35.0% | |
| TTAACTCACCATAAAGATGC+AGG | + | chr8.1:27551892-27551911 | None:intergenic | 35.0% | |
| ! | ACTTTAGTTCCAATGGCATT+AGG | - | chr8.1:27551857-27551876 | MS.gene96680:CDS | 35.0% |
| ! | CTTTAGTTCCAATGGCATTA+GGG | - | chr8.1:27551858-27551877 | MS.gene96680:CDS | 35.0% |
| !!! | CTTGTATTTTTTGGAGCTTC+TGG | - | chr8.1:27551962-27551981 | MS.gene96680:CDS | 35.0% |
| AGAGAGAGAAGGTTCTTTGT+TGG | - | chr8.1:27552027-27552046 | MS.gene96680:CDS | 40.0% | |
| GAGAGAGAAGGTTCTTTGTT+GGG | - | chr8.1:27552028-27552047 | MS.gene96680:CDS | 40.0% | |
| TCTGGACTTGGAATTGCATA+TGG | - | chr8.1:27551980-27551999 | MS.gene96680:CDS | 40.0% | |
| ! | TGGTGTGCTTTCTTCTAGTT+TGG | - | chr8.1:27552000-27552019 | MS.gene96680:CDS | 40.0% |
| !! | AGAAGAATTGGGTTGAGATG+TGG | - | chr8.1:27552064-27552083 | MS.gene96680:CDS | 40.0% |
| !!! | TTTTTTGGAGCTTCTGGACT+TGG | - | chr8.1:27551968-27551987 | MS.gene96680:CDS | 40.0% |
| AGTTTGGATGCAGAGAGAGA+AGG | - | chr8.1:27552016-27552035 | MS.gene96680:CDS | 45.0% | |
| GTCACAAATGGCATCCACAA+AGG | + | chr8.1:27551938-27551957 | None:intergenic | 45.0% | |
| TCTTGTGTTGGAGAAGCCTT+TGG | + | chr8.1:27551714-27551733 | None:intergenic | 45.0% | |
| !! | TGTTGATGGTGGTCTTGTGT+TGG | + | chr8.1:27551726-27551745 | None:intergenic | 45.0% |
| ! | AAGCCTTTGGCACTAGCTTG+TGG | + | chr8.1:27551701-27551720 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 27551698 | 27552102 | 27551698 | ID=MS.gene96680 |
| chr8.1 | mRNA | 27551698 | 27552102 | 27551698 | ID=MS.gene96680.t1;Parent=MS.gene96680 |
| chr8.1 | exon | 27551698 | 27552102 | 27551698 | ID=MS.gene96680.t1.exon1;Parent=MS.gene96680.t1 |
| chr8.1 | CDS | 27551698 | 27552102 | 27551698 | ID=cds.MS.gene96680.t1;Parent=MS.gene96680.t1 |
| Gene Sequence |
| Protein sequence |
