AlfalfaGEDB | Msa0181100

M. sativa cultivar zhongmu-4 / Msa0181100

KEGG: K19044
GO: GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070647 GO:0071704 GO:0140096 GO:1901564
PFAM: PF13637.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0181100	sp\|Q4FE45\|XB33_ARATH	74.231	520	119	7	1	512	1	513	0.0	774

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0181100	A0A072VPZ6	98.047	512	10	0	1	512	1	512	0.0	1038

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0008960	Msa0181100	0.801492	8.777942e-49	-8.615850e-47
Msa0027020	Msa0181100	-0.812558	3.991974e-51	-8.615850e-47
Msa0037280	Msa0181100	0.813831	2.097446e-51	-8.615850e-47
Msa0042520	Msa0181100	-0.802135	6.476853e-49	-8.615850e-47
Msa0074860	Msa0181100	-0.806898	6.578752e-50	-8.615850e-47
Msa0075990	Msa0181100	-0.813869	2.057724e-51	-8.615850e-47
Msa0081610	Msa0181100	-0.803792	2.944050e-49	-8.615850e-47
Msa0087550	Msa0181100	0.830158	3.438202e-55	-8.615850e-47
Msa0097470	Msa0181100	-0.804036	2.619209e-49	-8.615850e-47
Msa0127350	Msa0181100	-0.805903	1.066381e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0181100	MtrunA17_Chr6g0472461	83.041	513	86	1	1	512	1	513	0.0	891
Msa0181100	MtrunA17_Chr1g0203651	97.449	392	10	0	121	512	1	392	0.0	792
Msa0181100	MtrunA17_Chr4g0073161	58.313	403	153	4	1	396	7	401	4.71e-162	469
Msa0181100	MtrunA17_Chr1g0203661	94.595	111	6	0	1	111	1	111	4.12e-70	220
Msa0181100	MtrunA17_Chr2g0328031	31.051	409	216	11	1	376	1	376	4.76e-42	155
Msa0181100	MtrunA17_Chr4g0031491	30.198	404	221	11	5	378	6	378	9.80e-40	149
Msa0181100	MtrunA17_Chr4g0010051	29.330	358	196	9	47	375	55	384	1.95e-32	129
Msa0181100	MtrunA17_Chr1g0196261	27.670	206	122	4	47	250	255	435	6.06e-13	70.9
Msa0181100	MtrunA17_Chr7g0276681	30.403	273	141	11	17	265	50	297	1.49e-12	70.1
Msa0181100	MtrunA17_Chr5g0409271	34.677	124	79	2	8	131	9	130	4.84e-12	66.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0181100	AT5G07270.1	74.231	520	119	7	1	512	1	513	0.0	774
Msa0181100	AT5G57740.1	55.990	384	156	4	1	379	7	382	7.28e-145	426
Msa0181100	AT2G28840.1	29.484	407	224	11	1	376	1	375	3.61e-39	148
Msa0181100	AT2G28840.2	29.518	332	172	10	75	376	62	361	3.95e-28	117
Msa0181100	AT4G19150.1	38.655	119	71	2	14	132	20	136	1.81e-13	70.9
Msa0181100	AT3G04710.3	29.412	221	128	5	49	261	283	483	2.50e-13	73.2
Msa0181100	AT3G04710.1	29.412	221	128	5	49	261	59	259	6.04e-13	71.2
Msa0181100	AT2G31820.1	27.097	310	166	12	14	307	193	458	6.76e-13	71.6
Msa0181100	AT1G07710.2	28.421	285	154	11	10	286	65	307	5.10e-12	68.6
Msa0181100	AT1G07710.1	28.421	285	154	11	10	286	65	307	5.10e-12	68.6

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Find 150 sgRNAs with CRISPR-Local

Find 291 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCATCTTCGCTTCCACA+AGG	0.645699	1_4:-69204714	None:intergenic
AAATCACAATCACAATCTCC+CGG	0.585082	1_4:-69208569	None:intergenic
AAGATGTATTAGACTTGTTG+TGG	0.638569	1_4:+69205569	Msa0181100:CDS
AAGCTTGTAGATATGGTCAT+TGG	0.430336	1_4:+69205356	Msa0181100:CDS
AAGGACAGGTAATTTGAATG+AGG	0.567827	1_4:-69206509	None:intergenic
AATAAATTACACACGTCTCT+TGG	0.473863	1_4:+69207928	Msa0181100:CDS
AATGTTTCTTCTGAAATGCT+TGG	0.462369	1_4:+69207823	Msa0181100:CDS
AATTTACAAAGCACAAATCT+AGG	0.450076	1_4:+69208703	Msa0181100:three_prime_UTR
ACAAAATTCAATCTCATCAG+AGG	0.568331	1_4:-69207384	None:intergenic
ACAATTCCATGTCTACAAAG+AGG	0.632660	1_4:-69207868	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAAATAAAAAGGTCAACGT+TGG	-	chr1_4:69204620-69204639	None:intergenic	30.0%
TCTAAGGGACACAAATAAAA+AGG	-	chr1_4:69204630-69204649	None:intergenic	30.0%
TTTATTTGTGTCCCTTAGAT+GGG	+	chr1_4:69204631-69204650	Msa0181100:exon	30.0%
GATTAGATGGTTACATTTAG+TGG	+	chr1_4:69205039-69205058	Msa0181100:intron	30.0%
TGTGTGGTCTATTCAATATT+AGG	+	chr1_4:69205482-69205501	Msa0181100:intron	30.0%
TTTGTAAATAAGACAGCTGA+TGG	+	chr1_4:69205903-69205922	Msa0181100:CDS	30.0%
ATTTAACGAAGCCATATGAA+GGG	-	chr1_4:69205940-69205959	None:intergenic	30.0%
TCTTCTGTGACATTTCATTA+TGG	+	chr1_4:69206008-69206027	Msa0181100:CDS	30.0%
CATGATATTGATATACCTTC+TGG	+	chr1_4:69206214-69206233	Msa0181100:intron	30.0%
AACGGGTAATTCATTTCAAA+GGG	+	chr1_4:69206320-69206339	Msa0181100:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	69204527	69208877	69204527	ID=Msa0181100;Name=Msa0181100
chr1_4	mRNA	69204527	69208877	69204527	ID=Msa0181100-mRNA-1;Parent=Msa0181100;Name=Msa0181100-mRNA-1;_AED=0.09;_eAED=0.09;_QI=122\|1\|1\|1\|1\|1\|10\|566\|512
chr1_4	exon	69204527	69204825	69204527	ID=Msa0181100-mRNA-1:exon:22983;Parent=Msa0181100-mRNA-1
chr1_4	exon	69204911	69204970	69204911	ID=Msa0181100-mRNA-1:exon:22984;Parent=Msa0181100-mRNA-1
chr1_4	exon	69205343	69205414	69205343	ID=Msa0181100-mRNA-1:exon:22985;Parent=Msa0181100-mRNA-1
chr1_4	exon	69205504	69205688	69205504	ID=Msa0181100-mRNA-1:exon:22986;Parent=Msa0181100-mRNA-1
chr1_4	exon	69205890	69206050	69205890	ID=Msa0181100-mRNA-1:exon:22987;Parent=Msa0181100-mRNA-1
chr1_4	exon	69206131	69206189	69206131	ID=Msa0181100-mRNA-1:exon:22988;Parent=Msa0181100-mRNA-1
chr1_4	exon	69206278	69206324	69206278	ID=Msa0181100-mRNA-1:exon:22989;Parent=Msa0181100-mRNA-1
chr1_4	exon	69206418	69206561	69206418	ID=Msa0181100-mRNA-1:exon:22990;Parent=Msa0181100-mRNA-1
chr1_4	exon	69207355	69207446	69207355	ID=Msa0181100-mRNA-1:exon:22991;Parent=Msa0181100-mRNA-1
chr1_4	exon	69207770	69208877	69207770	ID=Msa0181100-mRNA-1:exon:22992;Parent=Msa0181100-mRNA-1
chr1_4	five_prime_UTR	69204527	69204648	69204527	ID=Msa0181100-mRNA-1:five_prime_utr;Parent=Msa0181100-mRNA-1
chr1_4	CDS	69204649	69204825	69204649	ID=Msa0181100-mRNA-1:cds;Parent=Msa0181100-mRNA-1
chr1_4	CDS	69204911	69204970	69204911	ID=Msa0181100-mRNA-1:cds;Parent=Msa0181100-mRNA-1
chr1_4	CDS	69205343	69205414	69205343	ID=Msa0181100-mRNA-1:cds;Parent=Msa0181100-mRNA-1
chr1_4	CDS	69205504	69205688	69205504	ID=Msa0181100-mRNA-1:cds;Parent=Msa0181100-mRNA-1
chr1_4	CDS	69205890	69206050	69205890	ID=Msa0181100-mRNA-1:cds;Parent=Msa0181100-mRNA-1
chr1_4	CDS	69206131	69206189	69206131	ID=Msa0181100-mRNA-1:cds;Parent=Msa0181100-mRNA-1
chr1_4	CDS	69206278	69206324	69206278	ID=Msa0181100-mRNA-1:cds;Parent=Msa0181100-mRNA-1
chr1_4	CDS	69206418	69206561	69206418	ID=Msa0181100-mRNA-1:cds;Parent=Msa0181100-mRNA-1
chr1_4	CDS	69207355	69207446	69207355	ID=Msa0181100-mRNA-1:cds;Parent=Msa0181100-mRNA-1
chr1_4	CDS	69207770	69208311	69207770	ID=Msa0181100-mRNA-1:cds;Parent=Msa0181100-mRNA-1
chr1_4	three_prime_UTR	69208312	69208877	69208312	ID=Msa0181100-mRNA-1:three_prime_utr;Parent=Msa0181100-mRNA-1

Gene Sequence

>Msa0181100

ATGGGTAATTCTTTCGGATGTTCTGCTTCTGGCGAGAGACTGGTATCTGCGGCGAGGGATGGTGACCTTGTGGAAGCGAAGATGCTTTTGAATTGTAATCCTTATCTTGCTAAATACTCCACTTTTGGTGGTCTTAATTCTCCTCTTCATTTTGCATCTGCAAAGGGTCATAACGAGATTGTTGCTTTGTTGCTTGAGAATGGAGCTGATGTTAATTCAAGAAATTATTCTGGCCAGACGGCATTGATGCAAGCTTGTAGATATGGTCATTGGGAAGTTGTACAAACACTTTTACTCTTCAGATGCAATGTTATGAGAGCTGATTATCTTAGTGGGAGGACAGCTCTTCACTTTGCAGCGATGAACGGGCATGTAAGATGTATTAGACTTGTTGTGGCTGACTTTGTTCCAAGTGCTCCTTATGATGTGATACATGCTCGATCAGATGCTGAACAAGGTGGTGGATCAAATGTGAAAGGCAAACATGAACAAAGTGCTTTGTCCAAGTTTGTAAATAAGACAGCTGATGGTGGTATCACTGCCCTTCATATGGCTTCGTTAAATGGATACTTTGATTGTGTGCTACTGCTACTTGATCTTAATGCAAATATGTCTTCTGTGACATTTCATTATGGAACATCAATGGATTTAATAGGGGCTGGAAGCACTCCTTTGCATTACGCCGCATGTGGGGGAAATTTAAAATGCTGTCAGATCCTCCTTGCAAGAGGTGCAAGTCGGATGTCTTTGAATTGCAACGGGTGGCTTCCCCTTGATATTGCTCGGATGTGGGGGCGTCATTGGCTTGAACCGTTGCTGGCACCCAGTTCTGATGCCGTAACACCTTCCTTCCCTCATTCAAATTACCTGTCCTTGCCTCTCATGAGTGTTCTCAACATAGCCAGAGAGTATGGATTACGATCATCTACAACTTCCTCTGATGAGATTGAATTTTGTGCTGTATGCTTGGAGAGGCCATGTTCAGTGGCTGCAGAAGGATGTGGACATGAACTTTGTGTGAGATGTGCGCTCTATCTTTGCTCGACAAGCAATGTTTCTTCTGAAATGCTTGGCCCTCCTGGCTCTATCCCTTGCCCTCTTTGTAGACATGGAATTGTCTCTTTTGTCAAGTTGCCAGGTTCCCAAGCAAAAGAAAATAAATTACACACGTCTCTTGGCCTCTGCACTCCATGCATGTTACATCCACGCGACATAGATCAGCCATCTCTTTCTCACACACCAGAGATCCAAAGAAACTGTGTTGATTCTGTTCCTTCAGAGTTACTCTGTCCCGTTACATGTAGTCCATTTCCATCTATGGCAATACCTCTATGCACCTGCAACGAAGGTTCGTGTCCATCATTTGAACCACGAGACGTAGAAACAAGAGACGAATCACCTCGTCATTCACAGACATCAACAACAGATCAGGATAAAATTGAAGGGCCGAGACTGGATAAAACAACTTGCTCGAACATGTTTTGGGGTAGAAGAAGTTGCAGCAGGGAGCACCAATGCAATTCGGAAATAAATGCTTGA

Protein sequence

>Msa0181100

MGNSFGCSASGERLVSAARDGDLVEAKMLLNCNPYLAKYSTFGGLNSPLHFASAKGHNEIVALLLENGADVNSRNYSGQTALMQACRYGHWEVVQTLLLFRCNVMRADYLSGRTALHFAAMNGHVRCIRLVVADFVPSAPYDVIHARSDAEQGGGSNVKGKHEQSALSKFVNKTADGGITALHMASLNGYFDCVLLLLDLNANMSSVTFHYGTSMDLIGAGSTPLHYAACGGNLKCCQILLARGASRMSLNCNGWLPLDIARMWGRHWLEPLLAPSSDAVTPSFPHSNYLSLPLMSVLNIAREYGLRSSTTSSDEIEFCAVCLERPCSVAAEGCGHELCVRCALYLCSTSNVSSEMLGPPGSIPCPLCRHGIVSFVKLPGSQAKENKLHTSLGLCTPCMLHPRDIDQPSLSHTPEIQRNCVDSVPSELLCPVTCSPFPSMAIPLCTCNEGSCPSFEPRDVETRDESPRHSQTSTTDQDKIEGPRLDKTTCSNMFWGRRSCSREHQCNSEINA*