AlfalfaGEDB | Msa0820130

M. sativa cultivar zhongmu-4 / Msa0820130

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0820130	XP_003617003.1	87.770	139	14	2	1	139	1	136	7.10e-73	226

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0820130	G7KCQ2	87.770	139	14	2	1	139	1	136	3.39e-73	226

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa0820130	0.841100	5.830364e-58	-8.615850e-47
Msa0000800	Msa0820130	-0.852505	4.418881e-61	-8.615850e-47
Msa0001510	Msa0820130	0.881821	1.745632e-70	-8.615850e-47
Msa0001550	Msa0820130	0.880757	4.219688e-70	-8.615850e-47
Msa0002110	Msa0820130	0.916659	1.364902e-85	-8.615850e-47
Msa0002690	Msa0820130	0.914742	1.340881e-84	-8.615850e-47
Msa0002700	Msa0820130	0.907444	5.036432e-81	-8.615850e-47
Msa0003340	Msa0820130	0.863366	2.620475e-64	-8.615850e-47
Msa0004180	Msa0820130	0.934495	3.695735e-96	-8.615850e-47
Msa0005500	Msa0820130	0.835928	1.261036e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0820130	MtrunA17_Chr5g0439941	87.770	139	14	2	1	139	1	136	6.53e-77	226

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0820130	AT3G44020.1	66.265	83	20	2	62	139	58	137	7.80e-31	109

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Find 32 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCCATTAAACAGCAAAAT+GGG	0.370822	5_3:-57287755	None:intergenic
AAGGAAGAGAGAAAGAGCAA+TGG	0.658609	5_3:-57287840	None:intergenic
AATTATCAAGGACACATCAC+AGG	0.525647	5_3:-57290421	None:intergenic
AATTCAAAGCTCATAACCTT+CGG	0.437358	5_3:-57290286	None:intergenic
AGAGAAGATGTAGAAGAACA+AGG	0.567040	5_3:-57287635	None:intergenic
AGCCATTAAACAGCAAAATG+GGG	0.675128	5_3:-57287754	None:intergenic
AGGAGAAGAAGAAAGATGAA+TGG	0.496278	5_3:-57287615	None:intergenic
ATCCAGATTCTTGATGATCA+TGG	0.464032	5_3:+57287730	Msa0820130:CDS
ATTATCAAGGACACATCACA+GGG	0.688379	5_3:-57290420	None:intergenic
ATTCAAAGCTCATAACCTTC+GGG	0.418762	5_3:-57290285	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGCCATTAAACAGCAAAAT+GGG	-	chr5_3:57287758-57287777	None:intergenic	30.0%
TAAGCCATTAAACAGCAAAA+TGG	-	chr5_3:57287759-57287778	None:intergenic	30.0%
TTTGGTAAAAACGTATCAGA+TGG	-	chr5_3:57288358-57288377	None:intergenic	30.0%
TAGTAACCAAAAAGAACCTT+AGG	+	chr5_3:57288396-57288415	Msa0820130:intron	30.0%
AATAAACACATTTCTGCCTA+AGG	-	chr5_3:57288415-57288434	None:intergenic	30.0%
ATAGATGGAATTAGTCCATT+TGG	-	chr5_3:57288447-57288466	None:intergenic	30.0%
ATTCAATTATGAGTTTCTGC+TGG	+	chr5_3:57288614-57288633	Msa0820130:intron	30.0%
GATTGGAAAAACCTTTATTG+GGG	+	chr5_3:57288641-57288660	Msa0820130:intron	30.0%
GGTAATTATCTTTCTGCAAT+TGG	+	chr5_3:57288877-57288896	Msa0820130:intron	30.0%
GGGTAAGGAAAATAAAAACA+TGG	-	chr5_3:57289289-57289308	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	57287495	57290583	57287495	ID=Msa0820130;Name=Msa0820130
chr5_3	mRNA	57287495	57290583	57287495	ID=Msa0820130-mRNA-1;Parent=Msa0820130;Name=Msa0820130-mRNA-1;_AED=0.02;_eAED=0.02;_QI=65\|0.66\|0.75\|1\|1\|1\|4\|278\|158
chr5_3	exon	57287495	57287871	57287495	ID=Msa0820130-mRNA-1:exon:17309;Parent=Msa0820130-mRNA-1
chr5_3	exon	57288841	57288877	57288841	ID=Msa0820130-mRNA-1:exon:17310;Parent=Msa0820130-mRNA-1
chr5_3	exon	57289774	57289841	57289774	ID=Msa0820130-mRNA-1:exon:17311;Parent=Msa0820130-mRNA-1
chr5_3	exon	57290246	57290583	57290246	ID=Msa0820130-mRNA-1:exon:17312;Parent=Msa0820130-mRNA-1
chr5_3	five_prime_UTR	57287495	57287559	57287495	ID=Msa0820130-mRNA-1:five_prime_utr;Parent=Msa0820130-mRNA-1
chr5_3	CDS	57287560	57287871	57287560	ID=Msa0820130-mRNA-1:cds;Parent=Msa0820130-mRNA-1
chr5_3	CDS	57288841	57288877	57288841	ID=Msa0820130-mRNA-1:cds;Parent=Msa0820130-mRNA-1
chr5_3	CDS	57289774	57289841	57289774	ID=Msa0820130-mRNA-1:cds;Parent=Msa0820130-mRNA-1
chr5_3	CDS	57290246	57290305	57290246	ID=Msa0820130-mRNA-1:cds;Parent=Msa0820130-mRNA-1
chr5_3	three_prime_UTR	57290306	57290583	57290306	ID=Msa0820130-mRNA-1:three_prime_utr;Parent=Msa0820130-mRNA-1

Gene Sequence

>Msa0820130

ATGGCCACGCTTTTTCACTGCACTCTTCATCCTCTATCTTCTTCTTCTTCAAGCTCCATTCATCTTTCTTCTTCTCCTTGTTCTTCTACATCTTCTCTTACACTTCCACAACCTTCTTCTTCATCCAAGTTCTACTTCCAATCCTCCAAATGCAGACGAAAACCAACAAAATCCAGATTCTTGATGATCATGGACCCCATTTTGCTGTTTAATGGCTTAGGTAGTAGTGCTTTCTATATTGATACACAAACACTTCTTGCTACTGTTAGTGTTTTGGCTGCCATTGCTCTTTCTCTCTTCCTTGGCTTCAAGGGTGATCCGGTGTCCTGTGAGCGATGTGGTGGCAACGGTGGAACAAAATGTGTTTTTTGCAACAACGGCAAGATGAAAAAAGAGACTGGGTTGGTTGACTGCAAAGATTGGTACTCTGCAAGAAATGTGCAGGTTCTGGCTATTCCCGAAGGTTATGAGCTTTGA

Protein sequence

>Msa0820130

MATLFHCTLHPLSSSSSSSIHLSSSPCSSTSSLTLPQPSSSSKFYFQSSKCRRKPTKSRFLMIMDPILLFNGLGSSAFYIDTQTLLATVSVLAAIALSLFLGFKGDPVSCERCGGNGGTKCVFCNNGKMKKETGLVDCKDWYSARNVQVLAIPEGYEL*