Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0215840 | RHN75073.1 | 91.566 | 83 | 5 | 1 | 7 | 87 | 1 | 83 | 7.47e-39 | 135 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0215840 | A0A396JFK5 | 91.566 | 83 | 5 | 1 | 7 | 87 | 1 | 83 | 3.57e-39 | 135 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017510 | Msa0215840 | 0.819054 | 1.420296e-52 | -8.615850e-47 |
| Msa0095380 | Msa0215840 | 0.800225 | 1.592802e-48 | -8.615850e-47 |
| Msa0110970 | Msa0215840 | 0.809840 | 1.550966e-50 | -8.615850e-47 |
| Msa0150270 | Msa0215840 | 0.806334 | 8.651648e-50 | -8.615850e-47 |
| Msa0157290 | Msa0215840 | 0.818465 | 1.933103e-52 | -8.615850e-47 |
| Msa0188630 | Msa0215840 | 0.816468 | 5.446012e-52 | -8.615850e-47 |
| Msa0191250 | Msa0215840 | 0.810742 | 9.907429e-51 | -8.615850e-47 |
| Msa0215830 | Msa0215840 | 0.801154 | 1.029478e-48 | -8.615850e-47 |
| Msa0215840 | Msa0222920 | 0.804622 | 1.977942e-49 | -8.615850e-47 |
| Msa0215840 | Msa0230770 | 0.800114 | 1.677282e-48 | -8.615850e-47 |
| Msa0215840 | Msa0270100 | 0.803298 | 3.726079e-49 | -8.615850e-47 |
| Msa0215840 | Msa0270270 | 0.808754 | 2.651471e-50 | -8.615850e-47 |
| Msa0215840 | Msa0311860 | 0.801184 | 1.014722e-48 | -8.615850e-47 |
| Msa0215840 | Msa0320100 | 0.811367 | 7.253527e-51 | -8.615850e-47 |
| Msa0215840 | Msa0321860 | 0.820019 | 8.556892e-53 | -8.615850e-47 |
| Msa0215840 | Msa0323080 | 0.803331 | 3.669195e-49 | -8.615850e-47 |
| Msa0215840 | Msa0333030 | 0.800661 | 1.298067e-48 | -8.615850e-47 |
| Msa0215840 | Msa0333720 | -0.803269 | 3.778204e-49 | -8.615850e-47 |
| Msa0215840 | Msa0344830 | 0.801199 | 1.007544e-48 | -8.615850e-47 |
| Msa0215840 | Msa0369850 | 0.801041 | 1.085391e-48 | -8.615850e-47 |
| Msa0215840 | Msa0410500 | 0.801750 | 7.770450e-49 | -8.615850e-47 |
| Msa0215840 | Msa0432770 | 0.815346 | 9.692773e-52 | -8.615850e-47 |
| Msa0215840 | Msa0436050 | 0.800228 | 1.590147e-48 | -8.615850e-47 |
| Msa0215840 | Msa0480000 | 0.809766 | 1.609167e-50 | -8.615850e-47 |
| Msa0215840 | Msa0507680 | 0.805598 | 1.235814e-49 | -8.615850e-47 |
| Msa0215840 | Msa0519000 | 0.801200 | 1.007418e-48 | -8.615850e-47 |
| Msa0215840 | Msa0593650 | 0.801938 | 7.110288e-49 | -8.615850e-47 |
| Msa0215840 | Msa0598460 | 0.803179 | 3.944543e-49 | -8.615850e-47 |
| Msa0215840 | Msa0621590 | 0.814135 | 1.797126e-51 | -8.615850e-47 |
| Msa0215840 | Msa0638070 | 0.812538 | 4.030560e-51 | -8.615850e-47 |
| Msa0215840 | Msa0638280 | 0.819012 | 1.452155e-52 | -8.615850e-47 |
| Msa0215840 | Msa0638320 | 0.809610 | 1.737477e-50 | -8.615850e-47 |
| Msa0215840 | Msa0689010 | 0.806917 | 6.517209e-50 | -8.615850e-47 |
| Msa0215840 | Msa0691380 | 0.801006 | 1.103813e-48 | -8.615850e-47 |
| Msa0215840 | Msa0692230 | 0.820479 | 6.712336e-53 | -8.615850e-47 |
| Msa0215840 | Msa0693380 | 0.800996 | 1.108626e-48 | -8.615850e-47 |
| Msa0215840 | Msa0743260 | 0.824376 | 8.345252e-54 | -8.615850e-47 |
| Msa0215840 | Msa0743720 | 0.812891 | 3.374403e-51 | -8.615850e-47 |
| Msa0215840 | Msa0744040 | 0.813722 | 2.216473e-51 | -8.615850e-47 |
| Msa0215840 | Msa0754720 | -0.809602 | 1.745163e-50 | -8.615850e-47 |
| Msa0215840 | Msa0756630 | -0.801839 | 7.451236e-49 | -8.615850e-47 |
| Msa0215840 | Msa0778320 | 0.818112 | 2.323162e-52 | -8.615850e-47 |
| Msa0215840 | Msa0790510 | 0.803494 | 3.393423e-49 | -8.615850e-47 |
| Msa0215840 | Msa0829620 | 0.811824 | 5.769743e-51 | -8.615850e-47 |
| Msa0215840 | Msa0850960 | 0.827236 | 1.749236e-54 | -8.615850e-47 |
| Msa0215840 | Msa0863370 | 0.808855 | 2.523268e-50 | -8.615850e-47 |
| Msa0215840 | Msa0867890 | 0.812031 | 5.201752e-51 | -8.615850e-47 |
| Msa0215840 | Msa0880140 | 0.817327 | 3.492759e-52 | -8.615850e-47 |
| Msa0215840 | Msa0881020 | 0.805672 | 1.192300e-49 | -8.615850e-47 |
| Msa0215840 | Msa0881030 | 0.802845 | 4.623049e-49 | -8.615850e-47 |
| Msa0215840 | Msa0937670 | 0.808100 | 3.656288e-50 | -8.615850e-47 |
| Msa0215840 | Msa0944190 | 0.802799 | 4.727015e-49 | -8.615850e-47 |
| Msa0215840 | Msa1023400 | 0.800712 | 1.266992e-48 | -8.615850e-47 |
| Msa0215840 | Msa1040120 | 0.801096 | 1.057996e-48 | -8.615850e-47 |
| Msa0215840 | Msa1082500 | 0.806458 | 8.144538e-50 | -8.615850e-47 |
| Msa0215840 | Msa1090260 | 0.802928 | 4.445413e-49 | -8.615850e-47 |
| Msa0215840 | Msa1097800 | 0.807992 | 3.853917e-50 | -8.615850e-47 |
| Msa0215840 | Msa1111110 | 0.801244 | 9.865947e-49 | -8.615850e-47 |
| Msa0215840 | Msa1116130 | -0.808090 | 3.673274e-50 | -8.615850e-47 |
| Msa0215840 | Msa1151910 | 0.804384 | 2.216407e-49 | -8.615850e-47 |
| Msa0215840 | Msa1170280 | 0.804312 | 2.294403e-49 | -8.615850e-47 |
| Msa0215840 | Msa1171870 | 0.811483 | 6.844895e-51 | -8.615850e-47 |
| Msa0215840 | Msa1203250 | 0.818012 | 2.447390e-52 | -8.615850e-47 |
| Msa0215840 | Msa1217860 | 0.806718 | 7.178765e-50 | -8.615850e-47 |
| Msa0215840 | Msa1222970 | 0.805277 | 1.442510e-49 | -8.615850e-47 |
| Msa0215840 | Msa1233890 | 0.809451 | 1.879692e-50 | -8.615850e-47 |
| Msa0215840 | Msa1238930 | 0.814946 | 1.188738e-51 | -8.615850e-47 |
| Msa0215840 | Msa1264020 | 0.810165 | 1.320322e-50 | -8.615850e-47 |
| Msa0215840 | Msa1306430 | 0.810193 | 1.301963e-50 | -8.615850e-47 |
| Msa0215840 | Msa1308380 | 0.801966 | 7.017315e-49 | -8.615850e-47 |
| Msa0215840 | Msa1327050 | 0.803238 | 3.834139e-49 | -8.615850e-47 |
| Msa0215840 | Msa1348490 | 0.801080 | 1.065851e-48 | -8.615850e-47 |
| Msa0215840 | Msa1351730 | 0.806783 | 6.956714e-50 | -8.615850e-47 |
| Msa0215840 | Msa1359040 | 0.803342 | 3.649838e-49 | -8.615850e-47 |
| Msa0215840 | Msa1367080 | 0.810731 | 9.963089e-51 | -8.615850e-47 |
| Msa0215840 | Msa1371430 | 0.816728 | 4.760763e-52 | -8.615850e-47 |
| Msa0215840 | Msa1415640 | 0.812796 | 3.540212e-51 | -8.615850e-47 |
| Msa0215840 | Msa1416150 | 0.806713 | 7.197150e-50 | -8.615850e-47 |
| Msa0215840 | Msa1439960 | 0.807028 | 6.173437e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0215840 | MtrunA17_Chr2g0317191 | 91.566 | 83 | 5 | 1 | 7 | 87 | 1 | 83 | 6.87e-43 | 135 |
| Msa0215840 | MtrunA17_Chr5g0399351 | 66.667 | 81 | 24 | 2 | 7 | 87 | 3 | 80 | 9.19e-24 | 92.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0215840 | AT5G05210.1 | 57.627 | 59 | 24 | 1 | 29 | 87 | 24 | 81 | 3.54e-13 | 63.5 |
| Msa0215840 | AT5G05210.2 | 57.627 | 59 | 24 | 1 | 29 | 87 | 16 | 73 | 4.02e-13 | 63.5 |
Find 18 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACGAGAAGCCATGGTTTC+AGG | 0.217653 | 2_1:-61367717 | Msa0215840:CDS |
| CTGAAGGGTGTTGCAGATAT+TGG | 0.394867 | 2_1:-61367839 | Msa0215840:CDS |
| GCTGTTACAATGAGAGATTC+CGG | 0.414625 | 2_1:-61367884 | None:intergenic |
| GTTGGTAGATAAAACTTAGC+TGG | 0.463279 | 2_1:+61367746 | Msa0215820:intron |
| AACGAGAAGCCATGGTTTCA+GGG | 0.477522 | 2_1:-61367716 | Msa0215840:CDS |
| TATTGGTGCTTGTCAAGACT+TGG | 0.520276 | 2_1:-61367822 | Msa0215840:CDS |
| GTTAGGGTCTGAACTCTAAA+CGG | 0.521104 | 2_1:+61367624 | Msa0215820:intron |
| GGCTTCTCGTTATCATCAGT+TGG | 0.521411 | 2_1:+61367728 | Msa0215820:intron |
| AATGAGAGATTCCGGAGTGA+TGG | 0.528245 | 2_1:-61367876 | Msa0215840:CDS |
| TTGCTTAGACCCTGAAACCA+TGG | 0.533554 | 2_1:+61367707 | Msa0215820:intron |
| GGAGATTAAGAAGAAGCTGA+AGG | 0.558266 | 2_1:-61367855 | Msa0215840:CDS |
| AAGCAAGGGTGCAAAAGCTA+AGG | 0.564446 | 2_1:-61367690 | Msa0215840:CDS |
| GAGATTAAGAAGAAGCTGAA+GGG | 0.579276 | 2_1:-61367854 | Msa0215840:CDS |
| TAGACAATATTATGAAGTCT+CGG | 0.586642 | 2_1:-61367652 | Msa0215840:CDS |
| ATGGTTTCAGGGTCTAAGCA+AGG | 0.591717 | 2_1:-61367705 | Msa0215840:CDS |
| CTTCTTAATCTCCATCACTC+CGG | 0.599988 | 2_1:+61367865 | Msa0215820:intron |
| CTGATGATAACGAGAAGCCA+TGG | 0.624704 | 2_1:-61367724 | Msa0215840:CDS |
| TGGTTTCAGGGTCTAAGCAA+GGG | 0.633440 | 2_1:-61367704 | Msa0215840:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GATCAATTTGTCGAAAAAAT+GGG | + | chr2_1:61367725-61367744 | Msa0215820:intron | 25.0% |
| ! | TAGACAATATTATGAAGTCT+CGG | - | chr2_1:61367848-61367867 | Msa0215840:CDS | 25.0% |
| CGATCAATTTGTCGAAAAAA+TGG | + | chr2_1:61367726-61367745 | Msa0215820:intron | 30.0% | |
| GAGATTAAGAAGAAGCTGAA+GGG | - | chr2_1:61367646-61367665 | Msa0215840:CDS | 35.0% | |
| ! | GTTGGTAGATAAAACTTAGC+TGG | + | chr2_1:61367757-61367776 | Msa0215820:intron | 35.0% |
| CTTCTTAATCTCCATCACTC+CGG | + | chr2_1:61367638-61367657 | Msa0215820:intron | 40.0% | |
| GGAGATTAAGAAGAAGCTGA+AGG | - | chr2_1:61367645-61367664 | Msa0215840:CDS | 40.0% | |
| ! | TATTGGTGCTTGTCAAGACT+TGG | - | chr2_1:61367678-61367697 | Msa0215840:CDS | 40.0% |
| !! | CTGAAGGGTGTTGCAGATAT+TGG | - | chr2_1:61367661-61367680 | Msa0215840:CDS | 45.0% |
| GGCTTCTCGTTATCATCAGT+TGG | + | chr2_1:61367775-61367794 | Msa0215820:intron | 45.0% | |
| ! | CTGATGATAACGAGAAGCCA+TGG | - | chr2_1:61367776-61367795 | Msa0215840:CDS | 45.0% |
| TAACGAGAAGCCATGGTTTC+AGG | - | chr2_1:61367783-61367802 | Msa0215840:CDS | 45.0% | |
| AACGAGAAGCCATGGTTTCA+GGG | - | chr2_1:61367784-61367803 | Msa0215840:CDS | 45.0% | |
| TTGCTTAGACCCTGAAACCA+TGG | + | chr2_1:61367796-61367815 | Msa0215820:intron | 45.0% | |
| ATGGTTTCAGGGTCTAAGCA+AGG | - | chr2_1:61367795-61367814 | Msa0215840:CDS | 45.0% | |
| TGGTTTCAGGGTCTAAGCAA+GGG | - | chr2_1:61367796-61367815 | Msa0215840:CDS | 45.0% | |
| ! | AAGCAAGGGTGCAAAAGCTA+AGG | - | chr2_1:61367810-61367829 | Msa0215840:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 61367625 | 61367897 | 61367625 | ID=Msa0215840;Name=Msa0215840 |
| chr2_1 | mRNA | 61367625 | 61367897 | 61367625 | ID=Msa0215840-mRNA-1;Parent=Msa0215840;Name=Msa0215840-mRNA-1;_AED=0.44;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|90 |
| chr2_1 | exon | 61367625 | 61367897 | 61367625 | ID=Msa0215840-mRNA-1:exon:16940;Parent=Msa0215840-mRNA-1 |
| chr2_1 | CDS | 61367625 | 61367897 | 61367625 | ID=Msa0215840-mRNA-1:cds;Parent=Msa0215840-mRNA-1 |
| Gene Sequence |
| Protein sequence |
