AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0215830


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0215830 MtrunA17_Chr5g0399351 61.929 197 48 4 10 179 109 305 2.88e-65 204
Msa0215830 MtrunA17_Chr2g0317181 79.208 101 19 1 91 191 1 99 2.29e-45 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0215830 AT5G05210.1 45.588 204 79 6 1 179 144 340 9.77e-41 142
Msa0215830 AT5G05210.2 45.588 204 79 6 1 179 136 332 1.13e-40 142
Msa0215830 AT2G27750.1 45.946 111 54 3 74 179 39 148 4.79e-20 83.2

Find 46 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTGAAGCTATTGCTAAAA+AGG 0.287342 2_1:-61367192 Msa0215830:CDS
ATACTTCTCTTTATCAGTTT+AGG 0.301257 2_1:+61367116 Msa0215820:intron
TGAAAGAAAGATAGAGTTTC+CGG 0.308185 2_1:-61367378 Msa0215830:CDS
AGGTCCTAACTGTGCAAATC+TGG 0.341251 2_1:-61367420 Msa0215830:CDS
ATCGCAAACTTGAAGAGTTT+CGG 0.345780 2_1:-61367445 Msa0215830:CDS
GCGGTTAAGCCTTTCATGTC+GGG 0.372922 2_1:-61367521 Msa0215830:CDS
AGCAGAAAATCGCAGTGAAA+TGG 0.374864 2_1:-61367073 Msa0215830:CDS
TGAAAGAGCTAAGAAGTTAG+AGG 0.400614 2_1:-61367240 Msa0215830:CDS
GTATACAGAAATGGAAGAAT+AGG 0.408150 2_1:-61367097 Msa0215830:CDS
GCAATAGCTTCACCCTTCTC+AGG 0.419537 2_1:+61367200 Msa0215820:intron
AGCAGCAATGAATAGAGCTT+CGG 0.425070 2_1:-61367159 Msa0215830:CDS
GCAGCAATGAATAGAGCTTC+GGG 0.426903 2_1:-61367158 Msa0215830:CDS
TCTTTAAAAGAGCTTGTGTT+TGG 0.437000 2_1:-61367326 Msa0215830:CDS
TCCTTTACCACCTTCTCCTC+CGG 0.440386 2_1:+61367359 Msa0215820:intron
ATCTGGAAAATGCTAATAAG+AGG 0.444036 2_1:-61367403 Msa0215830:CDS
TAAAGAGAAGTATACAGAAA+TGG 0.461758 2_1:-61367106 Msa0215830:CDS
GCAGAAAATCGCAGTGAAAT+GGG 0.472714 2_1:-61367072 Msa0215830:CDS
TCTTTCAAGTTCCTTGTGCT+TGG 0.476271 2_1:+61367256 Msa0215820:intron
GGCAATGTTAAACTTCAGAA+TGG 0.487362 2_1:-61367305 Msa0215830:CDS
GATAGAGTTTCCGGAGGAGA+AGG 0.496889 2_1:-61367369 Msa0215830:CDS
GAATAGAGCTTCGGGAATCA+AGG 0.497557 2_1:-61367150 Msa0215830:CDS
TGCGGTTAAGCCTTTCATGT+CGG 0.509135 2_1:-61367522 Msa0215830:CDS
TCCGGAGGAGAAGGTGGTAA+AGG 0.513098 2_1:-61367360 Msa0215830:CDS
AAACTTGAAGAGTTTCGGTC+AGG 0.519958 2_1:-61367440 Msa0215830:CDS
TAAGCCTTTCATGTCGGGGT+TGG 0.521349 2_1:-61367516 Msa0215830:CDS
TCAAACAAAGGATCAGTTGA+AGG 0.525525 2_1:-61367032 Msa0215830:CDS
CGCAGTGAAATGGGAAGAGA+GGG 0.547718 2_1:-61367063 Msa0215830:CDS
CCTTTCGTGTAGTTCTTCAA+AGG 0.548079 2_1:+61367472 Msa0215820:intron
GCTTCCAACCCCGACATGAA+AGG 0.556004 2_1:+61367512 Msa0215820:intron
CGGGGTTGGAAGCAGATGAT+CGG 0.561569 2_1:-61367502 Msa0215830:CDS
CTTCAGAATGGTGAAATGCA+AGG 0.562609 2_1:-61367293 Msa0215830:CDS
TTCAGAATGGTGAAATGCAA+GGG 0.568164 2_1:-61367292 Msa0215830:CDS
AGAGTTTCCGGAGGAGAAGG+TGG 0.570130 2_1:-61367366 Msa0215830:CDS
AATGACAGCGATGGAGAGGA+AGG 0.580649 2_1:-61367545 Msa0215830:CDS
CGGTTAAGCCTTTCATGTCG+GGG 0.581496 2_1:-61367520 Msa0215830:CDS
AATGAATGACAGCGATGGAG+AGG 0.585376 2_1:-61367549 Msa0215830:CDS
GAAGAAGAATGATCCTGAGA+AGG 0.589096 2_1:-61367213 Msa0215830:CDS
AAGAAGAATGATCCTGAGAA+GGG 0.591026 2_1:-61367212 Msa0215830:CDS
TCTGGAAAATGCTAATAAGA+GGG 0.594774 2_1:-61367402 Msa0215830:CDS
TCGCAGTGAAATGGGAAGAG+AGG 0.597560 2_1:-61367064 Msa0215830:CDS
AGGGTTCAAAGTTCAAACAA+AGG 0.644899 2_1:-61367044 Msa0215830:CDS
GAGAAAAGTTTCCAAGCACA+AGG 0.645702 2_1:-61367267 Msa0215830:CDS
GGAGGAGAAGGTGGTAAAGG+AGG 0.646856 2_1:-61367357 Msa0215830:CDS
AAGAAAGATAGAGTTTCCGG+AGG 0.655190 2_1:-61367375 Msa0215830:CDS
CCTTTGAAGAACTACACGAA+AGG 0.661334 2_1:-61367472 Msa0215830:CDS
CAGCGATGGAGAGGAAGGTG+CGG 0.700646 2_1:-61367540 Msa0215830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATACTTCTCTTTATCAGTTT+AGG + chr2_1:61367430-61367449 Msa0215820:intron 25.0%
!! TAAAGAGAAGTATACAGAAA+TGG - chr2_1:61367437-61367456 Msa0215830:CDS 25.0%
ATCTGGAAAATGCTAATAAG+AGG - chr2_1:61367140-61367159 Msa0215830:CDS 30.0%
TCTGGAAAATGCTAATAAGA+GGG - chr2_1:61367141-61367160 Msa0215830:CDS 30.0%
TGAAAGAAAGATAGAGTTTC+CGG - chr2_1:61367165-61367184 Msa0215830:CDS 30.0%
! TCTTTAAAAGAGCTTGTGTT+TGG - chr2_1:61367217-61367236 Msa0215830:CDS 30.0%
GTATACAGAAATGGAAGAAT+AGG - chr2_1:61367446-61367465 Msa0215830:CDS 30.0%
ATCGCAAACTTGAAGAGTTT+CGG - chr2_1:61367098-61367117 Msa0215830:CDS 35.0%
! TTTTCCAGATTTGCACAGTT+AGG + chr2_1:61367130-61367149 Msa0215820:intron 35.0%
!! AAGCTCTTTTAAAGACTCTG+TGG + chr2_1:61367212-61367231 Msa0215820:intron 35.0%
GGCAATGTTAAACTTCAGAA+TGG - chr2_1:61367238-61367257 Msa0215830:CDS 35.0%
TTCAGAATGGTGAAATGCAA+GGG - chr2_1:61367251-61367270 Msa0215830:CDS 35.0%
TGAAAGAGCTAAGAAGTTAG+AGG - chr2_1:61367303-61367322 Msa0215830:CDS 35.0%
AAGAAGAATGATCCTGAGAA+GGG - chr2_1:61367331-61367350 Msa0215830:CDS 35.0%
CTATTGCTAAAAAGGAAGCA+TGG - chr2_1:61367359-61367378 Msa0215830:CDS 35.0%
AGGGTTCAAAGTTCAAACAA+AGG - chr2_1:61367499-61367518 Msa0215830:CDS 35.0%
TCAAACAAAGGATCAGTTGA+AGG - chr2_1:61367511-61367530 Msa0215830:CDS 35.0%
AGGCAGAGAAACAACAAAAA+AGG - chr2_1:61367531-61367550 Msa0215830:CDS 35.0%
CCTTTCGTGTAGTTCTTCAA+AGG + chr2_1:61367074-61367093 Msa0215820:intron 40.0%
! CCTTTGAAGAACTACACGAA+AGG - chr2_1:61367071-61367090 Msa0215830:CDS 40.0%
AAACTTGAAGAGTTTCGGTC+AGG - chr2_1:61367103-61367122 Msa0215830:CDS 40.0%
AAGAAAGATAGAGTTTCCGG+AGG - chr2_1:61367168-61367187 Msa0215830:CDS 40.0%
CTTCAGAATGGTGAAATGCA+AGG - chr2_1:61367250-61367269 Msa0215830:CDS 40.0%
GAGAAAAGTTTCCAAGCACA+AGG - chr2_1:61367276-61367295 Msa0215830:CDS 40.0%
!! TCTTTCAAGTTCCTTGTGCT+TGG + chr2_1:61367290-61367309 Msa0215820:intron 40.0%
GAAGAAGAATGATCCTGAGA+AGG - chr2_1:61367330-61367349 Msa0215830:CDS 40.0%
GGGTGAAGCTATTGCTAAAA+AGG - chr2_1:61367351-61367370 Msa0215830:CDS 40.0%
AGCAGCAATGAATAGAGCTT+CGG - chr2_1:61367384-61367403 Msa0215830:CDS 40.0%
AGCAGAAAATCGCAGTGAAA+TGG - chr2_1:61367470-61367489 Msa0215830:CDS 40.0%
GCAGAAAATCGCAGTGAAAT+GGG - chr2_1:61367471-61367490 Msa0215830:CDS 40.0%
TGCGGTTAAGCCTTTCATGT+CGG - chr2_1:61367021-61367040 Msa0215830:CDS 45.0%
AGGTCCTAACTGTGCAAATC+TGG - chr2_1:61367123-61367142 Msa0215830:CDS 45.0%
GCAGCAATGAATAGAGCTTC+GGG - chr2_1:61367385-61367404 Msa0215830:CDS 45.0%
GAATAGAGCTTCGGGAATCA+AGG - chr2_1:61367393-61367412 Msa0215830:CDS 45.0%
AATGACAGCGATGGAGAGGA+AGG - chr2_1:61366998-61367017 Msa0215830:CDS 50.0%
GCGGTTAAGCCTTTCATGTC+GGG - chr2_1:61367022-61367041 Msa0215830:CDS 50.0%
CGGTTAAGCCTTTCATGTCG+GGG - chr2_1:61367023-61367042 Msa0215830:CDS 50.0%
TAAGCCTTTCATGTCGGGGT+TGG - chr2_1:61367027-61367046 Msa0215830:CDS 50.0%
GATAGAGTTTCCGGAGGAGA+AGG - chr2_1:61367174-61367193 Msa0215830:CDS 50.0%
TCCTTTACCACCTTCTCCTC+CGG + chr2_1:61367187-61367206 Msa0215820:intron 50.0%
GCAATAGCTTCACCCTTCTC+AGG + chr2_1:61367346-61367365 Msa0215820:intron 50.0%
TCGCAGTGAAATGGGAAGAG+AGG - chr2_1:61367479-61367498 Msa0215830:CDS 50.0%
CGCAGTGAAATGGGAAGAGA+GGG - chr2_1:61367480-61367499 Msa0215830:CDS 50.0%
GCTTCCAACCCCGACATGAA+AGG + chr2_1:61367034-61367053 Msa0215820:intron 55.0%
CGGGGTTGGAAGCAGATGAT+CGG - chr2_1:61367041-61367060 Msa0215830:CDS 55.0%
AGAGTTTCCGGAGGAGAAGG+TGG - chr2_1:61367177-61367196 Msa0215830:CDS 55.0%
TCCGGAGGAGAAGGTGGTAA+AGG - chr2_1:61367183-61367202 Msa0215830:CDS 55.0%
GGAGGAGAAGGTGGTAAAGG+AGG - chr2_1:61367186-61367205 Msa0215830:CDS 55.0%
CAGCGATGGAGAGGAAGGTG+CGG - chr2_1:61367003-61367022 Msa0215830:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 61366995 61367570 61366995 ID=Msa0215830;Name=Msa0215830
chr2_1 mRNA 61366995 61367570 61366995 ID=Msa0215830-mRNA-1;Parent=Msa0215830;Name=Msa0215830-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|191
chr2_1 exon 61366995 61367570 61366995 ID=Msa0215830-mRNA-1:exon:16937;Parent=Msa0215830-mRNA-1
chr2_1 CDS 61366995 61367570 61366995 ID=Msa0215830-mRNA-1:cds;Parent=Msa0215830-mRNA-1
Gene Sequence

>Msa0215830

ATGAATGACAGCGATGGAGAGGAAGGTGCGGTTAAGCCTTTCATGTCGGGGTTGGAAGCAGATGATCGGTTTACGACCTTTGAAGAACTACACGAAAGGCTTCATCGCAAACTTGAAGAGTTTCGGTCAGGTCCTAACTGTGCAAATCTGGAAAATGCTAATAAGAGGGATGAAAGAAAGATAGAGTTTCCGGAGGAGAAGGTGGTAAAGGAGGCCACAGAGTCTTTAAAAGAGCTTGTGTTTGGCAATGTTAAACTTCAGAATGGTGAAATGCAAGGGAAGAGAAAAGTTTCCAAGCACAAGGAACTTGAAAGAGCTAAGAAGTTAGAGGAAGTGAAGAAGAATGATCCTGAGAAGGGTGAAGCTATTGCTAAAAAGGAAGCATGGAAAGCAGCAATGAATAGAGCTTCGGGAATCAAGGTTCATGATGATCCTAAACTGATAAAGAGAAGTATACAGAAATGGAAGAATAGGCAGCAGAAAATCGCAGTGAAATGGGAAGAGAGGGTTCAAAGTTCAAACAAAGGATCAGTTGAAGGCAGAGAAACAACAAAAAAGGTATGCAAATATAGCTGA

Protein sequence

>Msa0215830

MNDSDGEEGAVKPFMSGLEADDRFTTFEELHERLHRKLEEFRSGPNCANLENANKRDERKIEFPEEKVVKEATESLKELVFGNVKLQNGEMQGKRKVSKHKELERAKKLEEVKKNDPEKGEAIAKKEAWKAAMNRASGIKVHDDPKLIKRSIQKWKNRQQKIAVKWEERVQSSNKGSVEGRETTKKVCKYS*