AlfalfaGEDB | Msa0716560

M. sativa cultivar zhongmu-4 / Msa0716560

KEGG: K19355
GO: GO:0000003 GO:0000272 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004567 GO:0005575 GO:0005576 GO:0005623 GO:0005975 GO:0005976 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009636 GO:0009664 GO:0009791 GO:0009827 GO:0009828 GO:0009845 GO:0009900 GO:0009987 GO:0010035 GO:0010038 GO:0010047 GO:0010154 GO:0010412 GO:0015923 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0016985 GO:0016998 GO:0022414 GO:0032501 GO:0032502 GO:0032504 GO:0042221 GO:0042545 GO:0043170 GO:0044036 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044464 GO:0045229 GO:0046355 GO:0046686 GO:0048046 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0061687 GO:0071554 GO:0071555 GO:0071585 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0090351 GO:0097501 GO:0098754 GO:1901575 GO:1990059 GO:1990170
PFAM: PF00150.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0716560	XP_003612065.1	98.858	438	5	0	1	438	1	438	0.0	901

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0716560	sp\|Q9FJZ3\|MAN7_ARATH	66.284	436	139	4	2	436	1	429	0.0	592

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0716560	G7KF03	98.858	438	5	0	1	438	1	438	0.0	901

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0008340	Msa0716560	0.820220	7.693686e-53	-8.615850e-47
Msa0011140	Msa0716560	0.848407	6.252005e-60	-8.615850e-47
Msa0011720	Msa0716560	0.808168	3.535615e-50	-8.615850e-47
Msa0011850	Msa0716560	0.804883	1.744265e-49	-8.615850e-47
Msa0015940	Msa0716560	0.810327	1.218279e-50	-8.615850e-47
Msa0050760	Msa0716560	0.808808	2.581222e-50	-8.615850e-47
Msa0105420	Msa0716560	0.819336	1.225061e-52	-8.615850e-47
Msa0110970	Msa0716560	0.805395	1.363075e-49	-8.615850e-47
Msa0120330	Msa0716560	0.817657	2.943172e-52	-8.615850e-47
Msa0716560	Msa0749550	0.802063	6.700089e-49	-8.615850e-47
Msa0716560	Msa0757950	0.939708	8.063060e-100	-8.615850e-47
Msa0716560	Msa0782970	0.817112	3.904838e-52	-8.615850e-47
Msa0716560	Msa0810480	0.947351	7.961446e-106	-8.615850e-47
Msa0716560	Msa0820670	0.809959	1.462222e-50	-8.615850e-47
Msa0716560	Msa0845590	-0.811426	7.044658e-51	-8.615850e-47
Msa0716560	Msa0862100	0.819458	1.149154e-52	-8.615850e-47
Msa0716560	Msa0893810	0.800380	1.480918e-48	-8.615850e-47
Msa0716560	Msa0896210	0.826088	3.286134e-54	-8.615850e-47
Msa0716560	Msa0944770	0.812284	4.580598e-51	-8.615850e-47
Msa0716560	Msa0951710	0.802604	5.184045e-49	-8.615850e-47
Msa0716560	Msa0955300	0.802102	6.579327e-49	-8.615850e-47
Msa0716560	Msa0996080	0.802133	6.483882e-49	-8.615850e-47
Msa0716560	Msa1008490	0.816358	5.762092e-52	-8.615850e-47
Msa0716560	Msa1009000	0.846808	1.721222e-59	-8.615850e-47
Msa0716560	Msa1014270	0.817538	3.130956e-52	-8.615850e-47
Msa0716560	Msa1020710	0.815433	9.266381e-52	-8.615850e-47
Msa0716560	Msa1055260	0.833561	4.970063e-56	-8.615850e-47
Msa0716560	Msa1055280	0.835666	1.469285e-56	-8.615850e-47
Msa0716560	Msa1098560	0.844120	9.202049e-59	-8.615850e-47
Msa0716560	Msa1099030	0.808119	3.622294e-50	-8.615850e-47
Msa0716560	Msa1124680	-0.801475	8.849921e-49	-8.615850e-47
Msa0716560	Msa1167400	0.804335	2.269800e-49	-8.615850e-47
Msa0716560	Msa1201470	0.802023	6.828491e-49	-8.615850e-47
Msa0716560	Msa1215960	0.813067	3.087242e-51	-8.615850e-47
Msa0716560	Msa1220230	0.804320	2.286279e-49	-8.615850e-47
Msa0716560	Msa1221560	0.805504	1.292812e-49	-8.615850e-47
Msa0716560	Msa1228210	0.821423	4.070834e-53	-8.615850e-47
Msa0716560	Msa1243010	0.812201	4.775359e-51	-8.615850e-47
Msa0716560	Msa1263660	0.821132	4.749999e-53	-8.615850e-47
Msa0716560	Msa1267220	0.819482	1.134894e-52	-8.615850e-47
Msa0716560	Msa1269080	0.939588	9.876282e-100	-8.615850e-47
Msa0716560	Msa1271280	0.838660	2.520420e-57	-8.615850e-47
Msa0716560	Msa1271300	0.813560	2.406143e-51	-8.615850e-47
Msa0716560	Msa1278840	0.829685	4.482374e-55	-8.615850e-47
Msa0716560	Msa1303720	0.840488	8.439683e-58	-8.615850e-47
Msa0716560	Msa1303780	0.843157	1.664963e-58	-8.615850e-47
Msa0716560	Msa1318240	0.810599	1.063771e-50	-8.615850e-47
Msa0716560	Msa1320740	0.808459	3.065441e-50	-8.615850e-47
Msa0716560	Msa1323060	0.800377	1.483242e-48	-8.615850e-47
Msa0716560	Msa1343820	0.812343	4.447597e-51	-8.615850e-47
Msa0716560	Msa1363120	0.807496	4.913311e-50	-8.615850e-47
Msa0716560	Msa1382720	0.816858	4.453309e-52	-8.615850e-47
Msa0716560	Msa1423450	0.817070	3.990600e-52	-8.615850e-47
Msa0716560	Msa1440600	0.808685	2.743349e-50	-8.615850e-47
Msa0716560	Msa1445790	0.802531	5.368818e-49	-8.615850e-47
Msa0716560	Msa1460890	0.807432	5.068357e-50	-8.615850e-47
Msa0136080	Msa0716560	0.849633	2.852261e-60	-8.615850e-47
Msa0136110	Msa0716560	0.853348	2.535689e-61	-8.615850e-47
Msa0140910	Msa0716560	0.807202	5.671091e-50	-8.615850e-47
Msa0151270	Msa0716560	0.829279	5.625768e-55	-8.615850e-47
Msa0185480	Msa0716560	0.809621	1.728388e-50	-8.615850e-47
Msa0197230	Msa0716560	0.823210	1.566118e-53	-8.615850e-47
Msa0371420	Msa0716560	-0.805163	1.524307e-49	-8.615850e-47
Msa0371540	Msa0716560	0.813913	2.012171e-51	-8.615850e-47
Msa0378340	Msa0716560	0.835538	1.582641e-56	-8.615850e-47
Msa0427690	Msa0716560	0.837017	6.660565e-57	-8.615850e-47
Msa0430820	Msa0716560	0.805940	1.047244e-49	-8.615850e-47
Msa0466060	Msa0716560	0.805150	1.533755e-49	-8.615850e-47
Msa0238890	Msa0716560	0.821896	3.163441e-53	-8.615850e-47
Msa0264350	Msa0716560	0.819847	9.367588e-53	-8.615850e-47
Msa0289890	Msa0716560	0.824611	7.349856e-54	-8.615850e-47
Msa0308830	Msa0716560	0.818194	2.225481e-52	-8.615850e-47
Msa0337330	Msa0716560	0.801545	8.561719e-49	-8.615850e-47
Msa0517340	Msa0716560	0.822345	2.489522e-53	-8.615850e-47
Msa0518520	Msa0716560	0.834452	2.973792e-56	-8.615850e-47
Msa0532770	Msa0716560	0.800610	1.329474e-48	-8.615850e-47
Msa0535450	Msa0716560	0.815475	9.072344e-52	-8.615850e-47
Msa0554710	Msa0716560	0.801862	7.371249e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0716560	MtrunA17_Chr5g0404601	98.858	438	5	0	1	438	1	438	0.0	901
Msa0716560	MtrunA17_Chr3g0141751	64.678	419	137	6	2	418	1	410	0.0	549
Msa0716560	MtrunA17_Chr1g0193611	57.179	397	166	2	7	403	11	403	2.17e-165	471
Msa0716560	MtrunA17_Chr1g0193601	57.252	393	164	2	11	403	15	403	2.15e-163	466
Msa0716560	MtrunA17_Chr1g0193531	56.061	396	168	4	9	404	11	400	1.04e-162	464
Msa0716560	MtrunA17_Chr4g0020521	57.182	369	156	2	31	399	34	400	2.06e-160	458
Msa0716560	MtrunA17_Chr1g0193551	52.854	403	186	4	1	403	6	404	5.42e-155	444
Msa0716560	MtrunA17_Chr7g0271511	54.987	371	162	3	29	399	60	425	6.28e-150	432
Msa0716560	MtrunA17_Chr8g0334501	52.455	387	181	2	24	408	55	440	2.57e-146	424
Msa0716560	MtrunA17_Chr7g0245101	51.958	383	181	2	31	411	60	441	4.57e-146	424
Msa0716560	MtrunA17_Chr7g0245051	49.630	405	195	3	15	411	37	440	7.09e-145	421
Msa0716560	MtrunA17_Chr2g0324441	46.791	374	195	2	30	399	39	412	1.99e-127	375
Msa0716560	MtrunA17_Chr1g0193541	47.044	406	174	5	5	408	8	374	2.23e-127	374
Msa0716560	MtrunA17_Chr4g0040121	43.236	377	211	1	31	404	39	415	6.02e-118	351

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0716560	AT5G66460.1	66.284	436	139	4	2	436	1	429	0.0	592
Msa0716560	AT3G10890.1	53.580	405	184	4	2	403	1	404	1.93e-168	479
Msa0716560	AT3G10900.1	54.568	405	177	6	2	402	1	402	1.72e-160	459
Msa0716560	AT5G01930.1	50.758	396	189	3	15	405	28	422	1.03e-147	428
Msa0716560	AT1G02310.1	52.368	380	169	5	26	399	24	397	2.59e-134	392
Msa0716560	AT1G02310.2	52.368	380	169	5	26	399	44	417	6.05e-134	392
Msa0716560	AT2G20680.1	46.133	375	200	1	30	402	41	415	7.05e-125	369
Msa0716560	AT4G28320.1	45.699	372	200	1	30	399	40	411	2.33e-124	368
Msa0716560	AT3G30540.1	48.333	360	140	7	2	355	1	320	6.05e-115	340

Find 120 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACAAATTGGTTTCTGAAAT+TGG	0.066566	5_1:-10030236	Msa0716560:exon
CTACAATGAACAAATGTTTA+AGG	0.138199	5_1:-10032873	Msa0716560:intron
GGCATTCATTTCATGTTAAA+TGG	0.157356	5_1:-10033076	Msa0716560:CDS
CCAATTCTAGTAGCAGAATT+TGG	0.190502	5_1:-10030588	Msa0716560:CDS
GGAAAATCATTTAAAGATTC+AGG	0.202299	5_1:-10030567	Msa0716560:CDS
GCAAAAGTGCATCTCTTTAA+TGG	0.203171	5_1:+10033311	None:intergenic
TTGCATAAATCTTCCGAATA+AGG	0.205768	5_1:+10030337	None:intergenic
TTTAGGAACCCTGTTGTTAA+AGG	0.213923	5_1:-10032422	Msa0716560:CDS
CAAATGGCTTTAATGCTTAC+TGG	0.225498	5_1:-10033039	Msa0716560:CDS
CTGATGTATTTAACCGAAAT+AGG	0.234530	5_1:-10029859	Msa0716560:three_prime_UTR
GTGAACAATTATGAGAGTTT+TGG	0.256433	5_1:-10032515	Msa0716560:CDS
TGGTTTCTGAAATTGGAATA+TGG	0.261008	5_1:-10030229	Msa0716560:exon
CCAAATTCTGCTACTAGAAT+TGG	0.275704	5_1:+10030588	None:intergenic
CATTGATAGGAATCACTTAT+TGG	0.286976	5_1:-10031244	Msa0716560:CDS
GTTAATAGTAGGATTGAATT+TGG	0.289349	5_1:+10031176	None:intergenic
GGAAATAGAGGAAGACAAAT+TGG	0.293388	5_1:-10030249	Msa0716560:CDS
CATCAGATGATGATTTCTTT+AGG	0.293457	5_1:-10032439	Msa0716560:CDS
TGATATTGAAACACTCTTGA+TGG	0.294230	5_1:+10033137	None:intergenic
CACAAAGCCCTAACCTATTT+CGG	0.301910	5_1:+10029846	None:intergenic
CTATTAAAAGTAAATAATTA+AGG	0.313308	5_1:-10029899	Msa0716560:three_prime_UTR
CTCATAAACTATACCTTATT+CGG	0.322951	5_1:-10030350	Msa0716560:CDS
CGTTTCTCTAATGTATTTCA+TGG	0.337333	5_1:-10029818	Msa0716560:three_prime_UTR
CAGCGAGTGGAGCATTGTTC+TGG	0.358097	5_1:-10030467	Msa0716560:CDS
ACACTCTTGATGGACCAAAA+TGG	0.361828	5_1:+10033147	None:intergenic
TTAAAGCCATTTGCATAGTA+AGG	0.366576	5_1:+10033049	None:intergenic
CTTTAGTGAAGTCCATTGAT+AGG	0.370341	5_1:-10031257	Msa0716560:CDS
GATATATGCGTCTGCGAAAA+GGG	0.371629	5_1:-10030496	Msa0716560:CDS
ACACTATATATCAATGTGTA+TGG	0.372842	5_1:+10033243	None:intergenic
GACTTACTGTTGCTAGAACT+TGG	0.374694	5_1:-10032943	Msa0716560:CDS
GTCAAAGGAAATTACAAGTA+AGG	0.378214	5_1:+10033370	None:intergenic
TGATCCATCTCAGAGATTCA+AGG	0.381469	5_1:-10032999	Msa0716560:CDS
GTATTTAACCGAAATAGGTT+AGG	0.386604	5_1:-10029854	Msa0716560:three_prime_UTR
CAAAAGTGCATCTCTTTAAT+GGG	0.390846	5_1:+10033312	None:intergenic
AACTGGGCTAGAAGTAATAA+TGG	0.393024	5_1:-10032473	Msa0716560:CDS
AGCTCCCATGCCATGATTGT+TGG	0.395030	5_1:+10031861	None:intergenic
ATGTAGAAACCTTTAACAAC+AGG	0.395168	5_1:+10032413	None:intergenic
GATGCCTGGGATTCGATTGT+TGG	0.395930	5_1:+10031131	None:intergenic
GCTTATTATTTGTGTTGTGT+TGG	0.398515	5_1:-10030195	Msa0716560:three_prime_UTR
TTTCTACATCAATCATGTTA+AGG	0.399179	5_1:-10032399	Msa0716560:intron
ATATAATTTAGTTACCCTCT+TGG	0.414515	5_1:+10030116	None:intergenic
TCCTTCAGGAAGGACTATTC+AGG	0.416711	5_1:-10031811	Msa0716560:intron
GTCCATGGCTTGTTGCTTCA+CGG	0.424293	5_1:+10032963	None:intergenic
ATACATCAGCAAACACAATC+TGG	0.428504	5_1:+10029873	None:intergenic
AGATATATGCGTCTGCGAAA+AGG	0.430041	5_1:-10030497	Msa0716560:CDS
TTATTGGAAGCTGGACTTGA+AGG	0.430930	5_1:-10031228	Msa0716560:CDS
CTTATTATTTGTGTTGTGTT+GGG	0.434370	5_1:-10030194	Msa0716560:three_prime_UTR
AGGTGTACGTCAGATCCTTC+AGG	0.440657	5_1:-10031825	Msa0716560:CDS
TCTCTTTAATGGGAAGTTGT+TGG	0.447013	5_1:+10033322	None:intergenic
TATTGAAAAGTTGATCTCTC+TGG	0.448941	5_1:+10030532	None:intergenic
GGAAGAAGCAGTATGTGAAC+TGG	0.448948	5_1:-10032490	Msa0716560:CDS
TGGAAAAGAGCTAGATTTAA+CGG	0.453928	5_1:-10030294	Msa0716560:CDS
AGTCCTTTGCAATATTCTCC+TGG	0.460187	5_1:-10032899	Msa0716560:CDS
GGATTGAATTTGGTCCTTTG+AGG	0.460580	5_1:+10031186	None:intergenic
AGTGAGTGGTAAGACTTAAG+AGG	0.463631	5_1:+10033284	None:intergenic
TTCCACAGACTGTTCTCAAC+AGG	0.466531	5_1:-10031920	Msa0716560:intron
GGAAATCCTTACTATGCAAA+TGG	0.467525	5_1:-10033055	Msa0716560:CDS
ATAGGGCTGGATCACGGAAA+TGG	0.471647	5_1:-10031283	Msa0716560:intron
TATTTAACCGAAATAGGTTA+GGG	0.471916	5_1:-10029853	Msa0716560:three_prime_UTR
CAATAAGTGATTCCTATCAA+TGG	0.476985	5_1:+10031245	None:intergenic
AATCCTACTATTAACAACAT+AGG	0.484474	5_1:-10031168	Msa0716560:CDS
ACGGTTGCAAAATCGATGCC+TGG	0.487815	5_1:+10031117	None:intergenic
GTCCCTATGTTGTTAATAGT+AGG	0.488737	5_1:+10031165	None:intergenic
AGAACTTGGGCTTTCAGTGA+TGG	0.491493	5_1:-10032929	Msa0716560:CDS
CAAAGATGATCCAACAATCA+TGG	0.501682	5_1:-10031871	Msa0716560:CDS
ATGCAATAAAACTAAAACTT+CGG	0.503597	5_1:+10029757	None:intergenic
AGGAATCACTTATTGGAAGC+TGG	0.504001	5_1:-10031237	Msa0716560:CDS
GAGCTAGATTTAACGGAAGA+AGG	0.504274	5_1:-10030287	Msa0716560:CDS
TTAGAGCATATTGTGCGTCT+AGG	0.506071	5_1:+10030616	None:intergenic
ACTTACTGTTGCTAGAACTT+GGG	0.508850	5_1:-10032942	Msa0716560:CDS
GAATCTTTCGACGATGGCTA+TGG	0.509733	5_1:-10030423	Msa0716560:CDS
TTCTGGCAACTTCTTACAGA+AGG	0.511415	5_1:-10030450	Msa0716560:CDS
GTTTCTCTAATGTATTTCAT+GGG	0.513529	5_1:-10029817	Msa0716560:three_prime_UTR
ATCCTACTATTAACAACATA+GGG	0.515967	5_1:-10031167	Msa0716560:CDS
GAAACCTTGAATCTCTGAGA+TGG	0.516474	5_1:+10032995	None:intergenic
TTCCGTGAAGCAACAAGCCA+TGG	0.522141	5_1:-10032965	Msa0716560:CDS
TCACGCGTTGGTGGAAATAG+AGG	0.525388	5_1:-10030261	Msa0716560:CDS
GCAACTTCTTACAGAAGGAA+TGG	0.529256	5_1:-10030445	Msa0716560:CDS
TATTGGAAGCTGGACTTGAA+GGG	0.536131	5_1:-10031227	Msa0716560:CDS
AATATCAATGTAGAAGCTGC+TGG	0.539763	5_1:-10033121	Msa0716560:CDS
CGGTTGCAAAATCGATGCCT+GGG	0.543074	5_1:+10031118	None:intergenic
TTGGACCATGAAAAGTGAAA+AGG	0.545370	5_1:+10033341	None:intergenic
CAGAACAATGCTCCACTCGC+TGG	0.548466	5_1:+10030468	None:intergenic
CACTATATATCAATGTGTAT+GGG	0.551211	5_1:+10033244	None:intergenic
ATTGCCAACAATCGAATCCC+AGG	0.555801	5_1:-10031135	Msa0716560:CDS
GTGAAAAGGATGTTTGTCAA+AGG	0.561022	5_1:+10033355	None:intergenic
GATGGCTATGGAATATCGCT+TGG	0.563874	5_1:-10030411	Msa0716560:CDS
TTCATTGTTATCCCGACCAA+TGG	0.564049	5_1:-10031095	Msa0716560:intron
TAAAACTAAAACTTCGGTGA+AGG	0.565929	5_1:+10029763	None:intergenic
ATGATCCAACAATCATGGCA+TGG	0.566474	5_1:-10031866	Msa0716560:CDS
ATTTGTTCATTGTAGAAACC+AGG	0.567175	5_1:+10032881	None:intergenic
CGCATATATCTTGTAGTACA+CGG	0.572125	5_1:+10030509	None:intergenic
ATATCTGAGGCTAGAAAGCA+TGG	0.573786	5_1:-10032560	Msa0716560:CDS
AATTAAGCTGATACTAAGCT+TGG	0.574063	5_1:-10032537	Msa0716560:CDS
TGATCCAACAATCATGGCAT+GGG	0.574244	5_1:-10031865	Msa0716560:CDS
AGAAGGTCTTCACGCGTTGG+TGG	0.576835	5_1:-10030270	Msa0716560:CDS
TTCTAGCAACAGTAAGTCCA+TGG	0.583102	5_1:+10032948	None:intergenic
ACTTGGGCTTTCAGTGATGG+TGG	0.586210	5_1:-10032926	Msa0716560:CDS
TTGTTTATTAGAGAAAGCTA+AGG	0.589413	5_1:-10030059	Msa0716560:three_prime_UTR
AAAAGGGGAGGTCCAGCGAG+TGG	0.590584	5_1:-10030480	Msa0716560:CDS
GGAAGAAGGTCTTCACGCGT+TGG	0.594873	5_1:-10030273	Msa0716560:CDS
TTGATTATAGGGCTGGATCA+CGG	0.597155	5_1:-10031289	Msa0716560:intron
ATGGACAATCAACACCTCAA+AGG	0.598022	5_1:-10031200	Msa0716560:CDS
AATGTAGAAGCTGCTGGTGA+TGG	0.601670	5_1:-10033115	Msa0716560:CDS
GAAGAAGCAGTATGTGAACT+GGG	0.610382	5_1:-10032489	Msa0716560:CDS
ACCTGAATAGTCCTTCCTGA+AGG	0.619157	5_1:+10031810	None:intergenic
GTGGTAAGACTTAAGAGGAA+AGG	0.620330	5_1:+10033289	None:intergenic
CAGAATGTTTACGTCCAAGA+GGG	0.622445	5_1:-10030130	Msa0716560:three_prime_UTR
ATATATGCGTCTGCGAAAAG+GGG	0.632807	5_1:-10030495	Msa0716560:CDS
AACAAACGAGAAGCCTGACA+TGG	0.638086	5_1:+10030077	None:intergenic
TGTAGAAACCTTTAACAACA+GGG	0.641407	5_1:+10032414	None:intergenic
TATGCGTCTGCGAAAAGGGG+AGG	0.642345	5_1:-10030492	Msa0716560:CDS
AAACCAGGAGAATATTGCAA+AGG	0.645714	5_1:+10032896	None:intergenic
ATACACATTGATATATAGTG+TGG	0.654755	5_1:-10033241	Msa0716560:five_prime_UTR
TCAGAATGTTTACGTCCAAG+AGG	0.658264	5_1:-10030131	Msa0716560:three_prime_UTR
TTGGTCGGGATAACAATGAA+CGG	0.658478	5_1:+10031098	None:intergenic
GAAGGATCTGACGTACACCT+AGG	0.660094	5_1:+10031828	None:intergenic
GGGAGCTTATGAATGAACCT+AGG	0.661088	5_1:-10031845	Msa0716560:CDS
GGAATGGAATCTTTCGACGA+TGG	0.663000	5_1:-10030429	Msa0716560:CDS
AGATCGCTAATGAACGACGA+TGG	0.665356	5_1:-10030314	Msa0716560:CDS
GTACGTCAGATCCTTCAGGA+AGG	0.713213	5_1:-10031821	Msa0716560:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTAAAAATTAATTTTGCATT+TGG	+	chr5_1:10031165-10031184	None:intergenic	10.0%
!!	ATATTTATATAATATATACG+AGG	-	chr5_1:10031216-10031235	Msa0716560:CDS	10.0%
!!	ATTGTAAACAAATTAAAAAT+GGG	+	chr5_1:10032210-10032229	None:intergenic	10.0%
!!	CTATTAAAAGTAAATAATTA+AGG	-	chr5_1:10033315-10033334	Msa0716560:five_prime_UTR	10.0%
!!!	TATTTTTCATAAACTATTCA+AGG	-	chr5_1:10030369-10030388	Msa0716560:CDS	15.0%
!!!	ACAATTTTCAACTTTTTTTA+CGG	+	chr5_1:10030508-10030527	None:intergenic	15.0%
!!	GTTCAATTTAATATATCATT+TGG	-	chr5_1:10030568-10030587	Msa0716560:CDS	15.0%
!!	TGAATATGAAAATTATCATA+TGG	-	chr5_1:10030880-10030899	Msa0716560:intron	15.0%
!!	TTAACTCATAAATAAGAATA+AGG	+	chr5_1:10031073-10031092	None:intergenic	15.0%
!!	AAAAAGAAAAAAAGAAAAAG+GGG	+	chr5_1:10031479-10031498	None:intergenic	15.0%
!!	CAAAAAGAAAAAAAGAAAAA+GGG	+	chr5_1:10031480-10031499	None:intergenic	15.0%
!!	TCAAAAAGAAAAAAAGAAAA+AGG	+	chr5_1:10031481-10031500	None:intergenic	15.0%
!!	TAATTGAAGTATAATTTCTT+TGG	+	chr5_1:10031578-10031597	None:intergenic	15.0%
!!!	ATTATGAACATTTTTTACAT+AGG	-	chr5_1:10031616-10031635	Msa0716560:intron	15.0%
!!	AATGATTTAACAATTATGAT+AGG	+	chr5_1:10031645-10031664	None:intergenic	15.0%
!!	CATTGTAAACAAATTAAAAA+TGG	+	chr5_1:10032211-10032230	None:intergenic	15.0%
!!!	TTATTCTAGACCTTTTTTTA+GGG	+	chr5_1:10029769-10029788	None:intergenic	20.0%
!!!	ATTATTCTAGACCTTTTTTT+AGG	+	chr5_1:10029770-10029789	None:intergenic	20.0%
!!!	TTTTGCTTTGTTATTTCTTT+TGG	-	chr5_1:10030044-10030063	Msa0716560:three_prime_UTR	20.0%
!!!	ATTTTTTTGTCTTAAACTTC+CGG	-	chr5_1:10030530-10030549	Msa0716560:CDS	20.0%
!!	CCTAATATGTAAAAACTAAA+AGG	+	chr5_1:10030940-10030959	None:intergenic	20.0%
!!!	CCTTTTAGTTTTTACATATT+AGG	-	chr5_1:10030937-10030956	Msa0716560:intron	20.0%
!!!	AAATTAATTTTGCATTTGGA+TGG	+	chr5_1:10031161-10031180	None:intergenic	20.0%
!!!	TTTGATGTAATTTATTTTCC+TGG	-	chr5_1:10031268-10031287	Msa0716560:CDS	20.0%
!!	TAAAGTCTTACCAATATTAT+TGG	-	chr5_1:10031798-10031817	Msa0716560:intron	20.0%
!!	ACTGTTTAACATTTGATTAT+AGG	-	chr5_1:10031913-10031932	Msa0716560:CDS	20.0%
!!	CTGTTTAACATTTGATTATA+GGG	-	chr5_1:10031914-10031933	Msa0716560:CDS	20.0%
!!!	ACTATATTTTTAGTTACCAT+TGG	+	chr5_1:10032138-10032157	None:intergenic	20.0%
!!!	ATTTGTTTACAATGCAATTT+TGG	-	chr5_1:10032217-10032236	Msa0716560:intron	20.0%
!!!	TTTGTTTACAATGCAATTTT+GGG	-	chr5_1:10032218-10032237	Msa0716560:intron	20.0%
!!	ATGCAATAAAACTAAAACTT+CGG	+	chr5_1:10033460-10033479	None:intergenic	20.0%
!!!	AAGTTTTAGTTTTATTGCAT+TGG	-	chr5_1:10033460-10033479	Msa0716560:five_prime_UTR	20.0%
!!!	AGTTTTAGTTTTATTGCATT+GGG	-	chr5_1:10033461-10033480	Msa0716560:five_prime_UTR	20.0%
!	CACTATATATCAATGTGTAT+GGG	+	chr5_1:10029973-10029992	None:intergenic	25.0%
!	ACACTATATATCAATGTGTA+TGG	+	chr5_1:10029974-10029993	None:intergenic	25.0%
!	ATACACATTGATATATAGTG+TGG	-	chr5_1:10029973-10029992	Msa0716560:three_prime_UTR	25.0%
!	CTACAATGAACAAATGTTTA+AGG	-	chr5_1:10030341-10030360	Msa0716560:CDS	25.0%
!!	TTGTATCTGATTTTCTATGT+TGG	-	chr5_1:10030605-10030624	Msa0716560:CDS	25.0%
!	TTTCTACATCAATCATGTTA+AGG	-	chr5_1:10030815-10030834	Msa0716560:intron	25.0%
!	CCAGTTTACGATTAAAAAAT+AGG	+	chr5_1:10031748-10031767	None:intergenic	25.0%
!!!	CCTATTTTTTAATCGTAAAC+TGG	-	chr5_1:10031745-10031764	Msa0716560:intron	25.0%
!	GTTTCTACTACCAATAATAT+TGG	+	chr5_1:10031811-10031830	None:intergenic	25.0%
!!	GAAACTTTTGCAATATGATT+TGG	-	chr5_1:10031826-10031845	Msa0716560:CDS	25.0%
!	GTTAATAGTAGGATTGAATT+TGG	+	chr5_1:10032041-10032060	None:intergenic	25.0%
!	AATCCTACTATTAACAACAT+AGG	-	chr5_1:10032046-10032065	Msa0716560:intron	25.0%
!	ATCCTACTATTAACAACATA+GGG	-	chr5_1:10032047-10032066	Msa0716560:intron	25.0%
!!!	TATTTTTAGTTACCATTGGT+CGG	+	chr5_1:10032134-10032153	None:intergenic	25.0%
!	CCAATTAAATCATGTACATT+TGG	+	chr5_1:10032452-10032471	None:intergenic	25.0%
!	CCAAATGTACATGATTTAAT+TGG	-	chr5_1:10032449-10032468	Msa0716560:CDS	25.0%
!	GGAAAATCATTTAAAGATTC+AGG	-	chr5_1:10032647-10032666	Msa0716560:intron	25.0%
!	CTCATAAACTATACCTTATT+CGG	-	chr5_1:10032864-10032883	Msa0716560:intron	25.0%
!	CTTATTATTTGTGTTGTGTT+GGG	-	chr5_1:10033020-10033039	Msa0716560:CDS	25.0%
!!!	GGAAGACATTTTTAAAGTAA+AGG	-	chr5_1:10033041-10033060	Msa0716560:CDS	25.0%
!	ATATAATTTAGTTACCCTCT+TGG	+	chr5_1:10033101-10033120	None:intergenic	25.0%
!	TTGTTTATTAGAGAAAGCTA+AGG	-	chr5_1:10033155-10033174	Msa0716560:CDS	25.0%
!	TATTTAACCGAAATAGGTTA+GGG	-	chr5_1:10033361-10033380	Msa0716560:five_prime_UTR	25.0%
!	GTTTCTCTAATGTATTTCAT+GGG	-	chr5_1:10033397-10033416	Msa0716560:five_prime_UTR	25.0%
	GTCAAAGGAAATTACAAGTA+AGG	+	chr5_1:10029847-10029866	None:intergenic	30.0%
!!	AACATCCTTTTCACTTTTCA+TGG	-	chr5_1:10029868-10029887	Msa0716560:three_prime_UTR	30.0%
!	CAAAAGTGCATCTCTTTAAT+GGG	+	chr5_1:10029905-10029924	None:intergenic	30.0%
!	TATTTTGTATTGTGAGTGAG+TGG	+	chr5_1:10029947-10029966	None:intergenic	30.0%
!!!	GTTATTTCTTTTGGCCATTT+TGG	-	chr5_1:10030053-10030072	Msa0716560:three_prime_UTR	30.0%
	TGATATTGAAACACTCTTGA+TGG	+	chr5_1:10030080-10030099	None:intergenic	30.0%
	GGCATTCATTTCATGTTAAA+TGG	-	chr5_1:10030138-10030157	Msa0716560:three_prime_UTR	30.0%
	TTAAAGCCATTTGCATAGTA+AGG	+	chr5_1:10030168-10030187	None:intergenic	30.0%
	ATTTGTTCATTGTAGAAACC+AGG	+	chr5_1:10030336-10030355	None:intergenic	30.0%
	TAATCTAACTTTGGATTACG+AGG	+	chr5_1:10030453-10030472	None:intergenic	30.0%
	TAATCCAAAGTTAGATTACG+AGG	-	chr5_1:10030455-10030474	Msa0716560:CDS	30.0%
	TTTACCTCGTAATCTAACTT+TGG	+	chr5_1:10030462-10030481	None:intergenic	30.0%
	TAAATTGAACAATTTCCGCA+GGG	+	chr5_1:10030558-10030577	None:intergenic	30.0%
	TTAAATTGAACAATTTCCGC+AGG	+	chr5_1:10030559-10030578	None:intergenic	30.0%
!	ATCTGATTTTCTATGTTGGT+TGG	-	chr5_1:10030609-10030628	Msa0716560:CDS	30.0%
!!	GTTGGATTTTGTTATATCTG+AGG	-	chr5_1:10030641-10030660	Msa0716560:CDS	30.0%
	AATTAAGCTGATACTAAGCT+TGG	-	chr5_1:10030677-10030696	Msa0716560:CDS	30.0%
!	GTGAACAATTATGAGAGTTT+TGG	-	chr5_1:10030699-10030718	Msa0716560:intron	30.0%
!!	AACAATTATGAGAGTTTTGG+AGG	-	chr5_1:10030702-10030721	Msa0716560:intron	30.0%
!!!	ACAATTATGAGAGTTTTGGA+GGG	-	chr5_1:10030703-10030722	Msa0716560:intron	30.0%
	CATCAGATGATGATTTCTTT+AGG	-	chr5_1:10030775-10030794	Msa0716560:intron	30.0%
	TGTAGAAACCTTTAACAACA+GGG	+	chr5_1:10030803-10030822	None:intergenic	30.0%
	ATGTAGAAACCTTTAACAAC+AGG	+	chr5_1:10030804-10030823	None:intergenic	30.0%
	AGATTAATTGGTTGTTGGAT+CGG	+	chr5_1:10031122-10031141	None:intergenic	30.0%
!!	ATTAGACCTCTGAGATTAAT+TGG	+	chr5_1:10031134-10031153	None:intergenic	30.0%
!	TAATTTTGCATTTGGATGGT+GGG	+	chr5_1:10031157-10031176	None:intergenic	30.0%
!	TTAATTTTGCATTTGGATGG+TGG	+	chr5_1:10031158-10031177	None:intergenic	30.0%
!!!	AGGAGTATAGTTTTGTTGTT+AGG	-	chr5_1:10031236-10031255	Msa0716560:CDS	30.0%
	AAGAAAAAAAGAAAAAGGGG+TGG	+	chr5_1:10031476-10031495	None:intergenic	30.0%
	TTAACATTTGATTATAGGGC+TGG	-	chr5_1:10031918-10031937	Msa0716560:intron	30.0%
	CAATAAGTGATTCCTATCAA+TGG	+	chr5_1:10031972-10031991	None:intergenic	30.0%
	CATTGATAGGAATCACTTAT+TGG	-	chr5_1:10031970-10031989	Msa0716560:intron	30.0%
!!!	ATTTTTAGTTACCATTGGTC+GGG	+	chr5_1:10032133-10032152	None:intergenic	30.0%
	AAGTGAATTTGACAGAATCA+TGG	+	chr5_1:10032424-10032443	None:intergenic	30.0%
!	TTTTCTTTGATTGCACAAAC+AGG	-	chr5_1:10032513-10032532	Msa0716560:CDS	30.0%
	TTTCTTTGATTGCACAAACA+GGG	-	chr5_1:10032514-10032533	Msa0716560:CDS	30.0%
!	TATTGAAAAGTTGATCTCTC+TGG	+	chr5_1:10032685-10032704	None:intergenic	30.0%
	TTGCATAAATCTTCCGAATA+AGG	+	chr5_1:10032880-10032899	None:intergenic	30.0%
	TGGAAAAGAGCTAGATTTAA+CGG	-	chr5_1:10032920-10032939	Msa0716560:CDS	30.0%
!!	GACAAATTGGTTTCTGAAAT+TGG	-	chr5_1:10032978-10032997	Msa0716560:CDS	30.0%
!!	TGGTTTCTGAAATTGGAATA+TGG	-	chr5_1:10032985-10033004	Msa0716560:CDS	30.0%
	GCTTATTATTTGTGTTGTGT+TGG	-	chr5_1:10033019-10033038	Msa0716560:CDS	30.0%
!	TTTTGCATCTACTTGTGTTT+TGG	-	chr5_1:10033194-10033213	Msa0716560:exon	30.0%
	CTGATGTATTTAACCGAAAT+AGG	-	chr5_1:10033355-10033374	Msa0716560:five_prime_UTR	30.0%
	GTATTTAACCGAAATAGGTT+AGG	-	chr5_1:10033360-10033379	Msa0716560:five_prime_UTR	30.0%
	CGTTTCTCTAATGTATTTCA+TGG	-	chr5_1:10033396-10033415	Msa0716560:five_prime_UTR	30.0%
	TAAAACTAAAACTTCGGTGA+AGG	+	chr5_1:10033454-10033473	None:intergenic	30.0%
	ATCTATCCCACCCTAAAAAA+AGG	-	chr5_1:10029756-10029775	Msa0716560:three_prime_UTR	35.0%
!!!	TCTAGACCTTTTTTTAGGGT+GGG	+	chr5_1:10029765-10029784	None:intergenic	35.0%
!!!	TTCTAGACCTTTTTTTAGGG+TGG	+	chr5_1:10029766-10029785	None:intergenic	35.0%
	GTGAAAAGGATGTTTGTCAA+AGG	+	chr5_1:10029862-10029881	None:intergenic	35.0%
	TTGGACCATGAAAAGTGAAA+AGG	+	chr5_1:10029876-10029895	None:intergenic	35.0%
	TCTCTTTAATGGGAAGTTGT+TGG	+	chr5_1:10029895-10029914	None:intergenic	35.0%
!	GCAAAAGTGCATCTCTTTAA+TGG	+	chr5_1:10029906-10029925	None:intergenic	35.0%
	AATATCAATGTAGAAGCTGC+TGG	-	chr5_1:10030093-10030112	Msa0716560:three_prime_UTR	35.0%
	GGAAATCCTTACTATGCAAA+TGG	-	chr5_1:10030159-10030178	Msa0716560:three_prime_UTR	35.0%
!!	CAAATGGCTTTAATGCTTAC+TGG	-	chr5_1:10030175-10030194	Msa0716560:three_prime_UTR	35.0%
	ACTTACTGTTGCTAGAACTT+GGG	-	chr5_1:10030272-10030291	Msa0716560:CDS	35.0%
	AAACCAGGAGAATATTGCAA+AGG	+	chr5_1:10030321-10030340	None:intergenic	35.0%
	TCTTAACCATCCTTGAGAAA+GGG	-	chr5_1:10030426-10030445	Msa0716560:CDS	35.0%
!!	TTCTATGTTGGTTGGTTTGA+AGG	-	chr5_1:10030617-10030636	Msa0716560:CDS	35.0%
!!	TCTATGTTGGTTGGTTTGAA+GGG	-	chr5_1:10030618-10030637	Msa0716560:CDS	35.0%
!	AACTGGGCTAGAAGTAATAA+TGG	-	chr5_1:10030741-10030760	Msa0716560:intron	35.0%
	TTTAGGAACCCTGTTGTTAA+AGG	-	chr5_1:10030792-10030811	Msa0716560:intron	35.0%
!	GAACTTCTTTTGTCTTTGCT+TGG	-	chr5_1:10030850-10030869	Msa0716560:intron	35.0%
!!	CTCTGAGATTAATTGGTTGT+TGG	+	chr5_1:10031127-10031146	None:intergenic	35.0%
	CAACAACCAATTAATCTCAG+AGG	-	chr5_1:10031125-10031144	Msa0716560:CDS	35.0%
!	AACAGGTACAACAGTTTTAC+TGG	-	chr5_1:10031311-10031330	Msa0716560:intron	35.0%
	CAAAGATGATCCAACAATCA+TGG	-	chr5_1:10031343-10031362	Msa0716560:intron	35.0%
	ACAGTCAATGAGAGGAATAA+AGG	+	chr5_1:10031516-10031535	None:intergenic	35.0%
	TGGACAAGTTTCACAAATCA+CGG	-	chr5_1:10031846-10031865	Msa0716560:CDS	35.0%
	TATAGCATGTCCTTGAAATC+AGG	+	chr5_1:10031873-10031892	None:intergenic	35.0%
	CTTTAGTGAAGTCCATTGAT+AGG	-	chr5_1:10031957-10031976	Msa0716560:intron	35.0%
	GTCCCTATGTTGTTAATAGT+AGG	+	chr5_1:10032052-10032071	None:intergenic	35.0%
!	AATGGGAGTTCTCGAATTTT+GGG	+	chr5_1:10032193-10032212	None:intergenic	35.0%
!	AAATGGGAGTTCTCGAATTT+TGG	+	chr5_1:10032194-10032213	None:intergenic	35.0%
!	AGCTTGCATTTTTGAACAAC+TGG	-	chr5_1:10032561-10032580	Msa0716560:CDS	35.0%
	CCAAATTCTGCTACTAGAAT+TGG	+	chr5_1:10032629-10032648	None:intergenic	35.0%
	CCAATTCTAGTAGCAGAATT+TGG	-	chr5_1:10032626-10032645	Msa0716560:intron	35.0%
	CGCATATATCTTGTAGTACA+CGG	+	chr5_1:10032708-10032727	None:intergenic	35.0%
	GGAAATAGAGGAAGACAAAT+TGG	-	chr5_1:10032965-10032984	Msa0716560:CDS	35.0%
	ATACATCAGCAAACACAATC+TGG	+	chr5_1:10033344-10033363	None:intergenic	35.0%
	GTGGTAAGACTTAAGAGGAA+AGG	+	chr5_1:10029928-10029947	None:intergenic	40.0%
	AGTGAGTGGTAAGACTTAAG+AGG	+	chr5_1:10029933-10029952	None:intergenic	40.0%
!	ACACTCTTGATGGACCAAAA+TGG	+	chr5_1:10030070-10030089	None:intergenic	40.0%
!!!	GATGGTTTTGTGAGAACAAG+AGG	-	chr5_1:10030117-10030136	Msa0716560:three_prime_UTR	40.0%
	TGATCCATCTCAGAGATTCA+AGG	-	chr5_1:10030215-10030234	Msa0716560:three_prime_UTR	40.0%
!	GAAACCTTGAATCTCTGAGA+TGG	+	chr5_1:10030222-10030241	None:intergenic	40.0%
	TTCTAGCAACAGTAAGTCCA+TGG	+	chr5_1:10030269-10030288	None:intergenic	40.0%
	GACTTACTGTTGCTAGAACT+TGG	-	chr5_1:10030271-10030290	Msa0716560:CDS	40.0%
	AGTCCTTTGCAATATTCTCC+TGG	-	chr5_1:10030315-10030334	Msa0716560:CDS	40.0%
	GTCTTAACCATCCTTGAGAA+AGG	-	chr5_1:10030425-10030444	Msa0716560:CDS	40.0%
!!	CTATGTTGGTTGGTTTGAAG+GGG	-	chr5_1:10030619-10030638	Msa0716560:CDS	40.0%
!	ATATCTGAGGCTAGAAAGCA+TGG	-	chr5_1:10030654-10030673	Msa0716560:CDS	40.0%
	GAAGAAGCAGTATGTGAACT+GGG	-	chr5_1:10030725-10030744	Msa0716560:intron	40.0%
	ATGATCCAACAATCATGGCA+TGG	-	chr5_1:10031348-10031367	Msa0716560:intron	40.0%
	TGATCCAACAATCATGGCAT+GGG	-	chr5_1:10031349-10031368	Msa0716560:intron	40.0%
!	TGAGAGGAATAAAGGTGTAG+TGG	+	chr5_1:10031508-10031527	None:intergenic	40.0%
	CATCTATCACAGTCAATGAG+AGG	+	chr5_1:10031524-10031543	None:intergenic	40.0%
	GGACAAGTTTCACAAATCAC+GGG	-	chr5_1:10031847-10031866	Msa0716560:CDS	40.0%
	TTGATTATAGGGCTGGATCA+CGG	-	chr5_1:10031925-10031944	Msa0716560:intron	40.0%
	AGGAATCACTTATTGGAAGC+TGG	-	chr5_1:10031977-10031996	Msa0716560:intron	40.0%
	TTATTGGAAGCTGGACTTGA+AGG	-	chr5_1:10031986-10032005	Msa0716560:intron	40.0%
	TATTGGAAGCTGGACTTGAA+GGG	-	chr5_1:10031987-10032006	Msa0716560:intron	40.0%
	ATGGACAATCAACACCTCAA+AGG	-	chr5_1:10032014-10032033	Msa0716560:intron	40.0%
	GGATTGAATTTGGTCCTTTG+AGG	+	chr5_1:10032031-10032050	None:intergenic	40.0%
	TTGGTCGGGATAACAATGAA+CGG	+	chr5_1:10032119-10032138	None:intergenic	40.0%
	TTCATTGTTATCCCGACCAA+TGG	-	chr5_1:10032119-10032138	Msa0716560:intron	40.0%
	TTAGAGCATATTGTGCGTCT+AGG	+	chr5_1:10032601-10032620	None:intergenic	40.0%
	AGATATATGCGTCTGCGAAA+AGG	-	chr5_1:10032717-10032736	Msa0716560:intron	40.0%
	GATATATGCGTCTGCGAAAA+GGG	-	chr5_1:10032718-10032737	Msa0716560:intron	40.0%
	ATATATGCGTCTGCGAAAAG+GGG	-	chr5_1:10032719-10032738	Msa0716560:intron	40.0%
	TTCTGGCAACTTCTTACAGA+AGG	-	chr5_1:10032764-10032783	Msa0716560:intron	40.0%
	GCAACTTCTTACAGAAGGAA+TGG	-	chr5_1:10032769-10032788	Msa0716560:intron	40.0%
	GAGCTAGATTTAACGGAAGA+AGG	-	chr5_1:10032927-10032946	Msa0716560:CDS	40.0%
	TCAGAATGTTTACGTCCAAG+AGG	-	chr5_1:10033083-10033102	Msa0716560:CDS	40.0%
	CAGAATGTTTACGTCCAAGA+GGG	-	chr5_1:10033084-10033103	Msa0716560:CDS	40.0%
	CACAAAGCCCTAACCTATTT+CGG	+	chr5_1:10033371-10033390	None:intergenic	40.0%
	AATGTAGAAGCTGCTGGTGA+TGG	-	chr5_1:10030099-10030118	Msa0716560:three_prime_UTR	45.0%
	AGAACTTGGGCTTTCAGTGA+TGG	-	chr5_1:10030285-10030304	Msa0716560:CDS	45.0%
	ATTACGAGGACCCTTTCTCA+AGG	+	chr5_1:10030439-10030458	None:intergenic	45.0%
	GGAAGAAGCAGTATGTGAAC+TGG	-	chr5_1:10030724-10030743	Msa0716560:intron	45.0%
	TTCCACAGACTGTTCTCAAC+AGG	-	chr5_1:10031294-10031313	Msa0716560:intron	45.0%
	TACCTGTTGAGAACAGTCTG+TGG	+	chr5_1:10031299-10031318	None:intergenic	45.0%
	GGGAGCTTATGAATGAACCT+AGG	-	chr5_1:10031369-10031388	Msa0716560:intron	45.0%
	TCCTTCAGGAAGGACTATTC+AGG	-	chr5_1:10031403-10031422	Msa0716560:intron	45.0%
	ACCTGAATAGTCCTTCCTGA+AGG	+	chr5_1:10031407-10031426	None:intergenic	45.0%
	AAATCACGGGCCTGATTTCA+AGG	-	chr5_1:10031860-10031879	Msa0716560:CDS	45.0%
	AAACAGTCAGACTCAGCTCA+TGG	+	chr5_1:10031900-10031919	None:intergenic	45.0%
!	GCTGGACTTGAAGGGTTTTA+TGG	-	chr5_1:10031995-10032014	Msa0716560:intron	45.0%
	ATTGCCAACAATCGAATCCC+AGG	-	chr5_1:10032079-10032098	Msa0716560:intron	45.0%
	GACAGAATCATGGTGAGCTA+TGG	+	chr5_1:10032414-10032433	None:intergenic	45.0%
	GGAATGGAATCTTTCGACGA+TGG	-	chr5_1:10032785-10032804	Msa0716560:intron	45.0%
	GAATCTTTCGACGATGGCTA+TGG	-	chr5_1:10032791-10032810	Msa0716560:intron	45.0%
	GATGGCTATGGAATATCGCT+TGG	-	chr5_1:10032803-10032822	Msa0716560:intron	45.0%
	AGATCGCTAATGAACGACGA+TGG	-	chr5_1:10032900-10032919	Msa0716560:CDS	45.0%
!!	CGTGTTTTTATCGCCATGTC+AGG	-	chr5_1:10033124-10033143	Msa0716560:CDS	45.0%
	AACAAACGAGAAGCCTGACA+TGG	+	chr5_1:10033140-10033159	None:intergenic	45.0%
	TTCCGTGAAGCAACAAGCCA+TGG	-	chr5_1:10030249-10030268	Msa0716560:CDS	50.0%
!!	GTCCATGGCTTGTTGCTTCA+CGG	+	chr5_1:10030254-10030273	None:intergenic	50.0%
	ACTTGGGCTTTCAGTGATGG+TGG	-	chr5_1:10030288-10030307	Msa0716560:CDS	50.0%
	CTTAAACTTCCGGTTCCCTG+CGG	-	chr5_1:10030540-10030559	Msa0716560:CDS	50.0%
	GAACAATTTCCGCAGGGAAC+CGG	+	chr5_1:10030552-10030571	None:intergenic	50.0%
!!	GTTGGTTGGTTTGAAGGGGT+TGG	-	chr5_1:10030623-10030642	Msa0716560:CDS	50.0%
	GAACAGTCTGTGGAAGTTCC+AGG	+	chr5_1:10031289-10031308	None:intergenic	50.0%
	AGCTCCCATGCCATGATTGT+TGG	+	chr5_1:10031356-10031375	None:intergenic	50.0%
	GAAGGATCTGACGTACACCT+AGG	+	chr5_1:10031389-10031408	None:intergenic	50.0%
	AGGTGTACGTCAGATCCTTC+AGG	-	chr5_1:10031389-10031408	Msa0716560:intron	50.0%
	GTACGTCAGATCCTTCAGGA+AGG	-	chr5_1:10031393-10031412	Msa0716560:intron	50.0%
	ATAGGGCTGGATCACGGAAA+TGG	-	chr5_1:10031931-10031950	Msa0716560:intron	50.0%
	GATGCCTGGGATTCGATTGT+TGG	+	chr5_1:10032086-10032105	None:intergenic	50.0%
	CGGTTGCAAAATCGATGCCT+GGG	+	chr5_1:10032099-10032118	None:intergenic	50.0%
	ACGGTTGCAAAATCGATGCC+TGG	+	chr5_1:10032100-10032119	None:intergenic	50.0%
!	TCACGCGTTGGTGGAAATAG+AGG	-	chr5_1:10032953-10032972	Msa0716560:CDS	50.0%
	CGAGGACCCTTTCTCAAGGA+TGG	+	chr5_1:10030435-10030454	None:intergenic	55.0%
	TATGCGTCTGCGAAAAGGGG+AGG	-	chr5_1:10032722-10032741	Msa0716560:intron	55.0%
	CAGAACAATGCTCCACTCGC+TGG	+	chr5_1:10032749-10032768	None:intergenic	55.0%
!!	CAGCGAGTGGAGCATTGTTC+TGG	-	chr5_1:10032747-10032766	Msa0716560:intron	55.0%
	GGAAGAAGGTCTTCACGCGT+TGG	-	chr5_1:10032941-10032960	Msa0716560:CDS	55.0%
	AGAAGGTCTTCACGCGTTGG+TGG	-	chr5_1:10032944-10032963	Msa0716560:CDS	55.0%
	AAAAGGGGAGGTCCAGCGAG+TGG	-	chr5_1:10032734-10032753	Msa0716560:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	10029752	10033484	10029752	ID=Msa0716560;Name=Msa0716560
chr5_1	mRNA	10029752	10033484	10029752	ID=Msa0716560-mRNA-1;Parent=Msa0716560;Name=Msa0716560-mRNA-1;_AED=0.03;_eAED=0.03;_QI=281\|1\|1\|1\|1\|1\|5\|490\|438
chr5_1	exon	10029752	10030701	10029752	ID=Msa0716560-mRNA-1:exon:4025;Parent=Msa0716560-mRNA-1
chr5_1	exon	10031096	10031301	10031096	ID=Msa0716560-mRNA-1:exon:4024;Parent=Msa0716560-mRNA-1
chr5_1	exon	10031812	10031934	10031812	ID=Msa0716560-mRNA-1:exon:4023;Parent=Msa0716560-mRNA-1
chr5_1	exon	10032400	10032597	10032400	ID=Msa0716560-mRNA-1:exon:4022;Parent=Msa0716560-mRNA-1
chr5_1	exon	10032874	10033484	10032874	ID=Msa0716560-mRNA-1:exon:4021;Parent=Msa0716560-mRNA-1
chr5_1	five_prime_UTR	10033204	10033484	10033204	ID=Msa0716560-mRNA-1:five_prime_utr;Parent=Msa0716560-mRNA-1
chr5_1	CDS	10032874	10033203	10032874	ID=Msa0716560-mRNA-1:cds;Parent=Msa0716560-mRNA-1
chr5_1	CDS	10032400	10032597	10032400	ID=Msa0716560-mRNA-1:cds;Parent=Msa0716560-mRNA-1
chr5_1	CDS	10031812	10031934	10031812	ID=Msa0716560-mRNA-1:cds;Parent=Msa0716560-mRNA-1
chr5_1	CDS	10031096	10031301	10031096	ID=Msa0716560-mRNA-1:cds;Parent=Msa0716560-mRNA-1
chr5_1	CDS	10030242	10030701	10030242	ID=Msa0716560-mRNA-1:cds;Parent=Msa0716560-mRNA-1
chr5_1	three_prime_UTR	10029752	10030241	10029752	ID=Msa0716560-mRNA-1:three_prime_utr;Parent=Msa0716560-mRNA-1

Gene Sequence

>Msa0716560

ATGATGAAGCATTTTGCTTTGTTATTTCTTTTGGCCATTTTGGTCCATCAAGAGTGTTTCAATATCAATGTAGAAGCTGCTGGTGATGGTTTTGTGAGAACAAGAGGCATTCATTTCATGTTAAATGGAAATCCTTACTATGCAAATGGCTTTAATGCTTACTGGTTAATGTATACAGCTTCTGATCCATCTCAGAGATTCAAGGTTTCAAATGCTTTCCGTGAAGCAACAAGCCATGGACTTACTGTTGCTAGAACTTGGGCTTTCAGTGATGGTGGTTATAGTCCTTTGCAATATTCTCCTGGTTTCTACAATGAACAAATGTTTAAGGGGTTGGATTTTGTTATATCTGAGGCTAGAAAGCATGGAATTAAGCTGATACTAAGCTTGGTGAACAATTATGAGAGTTTTGGAGGGAAGAAGCAGTATGTGAACTGGGCTAGAAGTAATAATGGACAGTATTTGACATCAGATGATGATTTCTTTAGGAACCCTGTTGTTAAAGGTTTCTACATCAATCATGTTAAGACTGTTCTCAACAGGTACAACAGTTTTACTGGAATTCATTACAAAGATGATCCAACAATCATGGCATGGGAGCTTATGAATGAACCTAGGTGTACGTCAGATCCTTCAGGAAGGACTATTCAGGGCTGGATCACGGAAATGGCTTCTTTAGTGAAGTCCATTGATAGGAATCACTTATTGGAAGCTGGACTTGAAGGGTTTTATGGACAATCAACACCTCAAAGGACCAAATTCAATCCTACTATTAACAACATAGGGACAGACTTCATTGCCAACAATCGAATCCCAGGCATCGATTTTGCAACCGTTCATTGTTATCCCGACCAATGGGTACAAAGTTCAAATGAGCAAGATCAGCTTGCATTTTTGAACAACTGGCTCAGTGCTCATTTCCTAGACGCACAATATGCTCTAAAAAAACCAATTCTAGTAGCAGAATTTGGAAAATCATTTAAAGATTCAGGTTTCAGTAGTAACCAGAGAGATCAACTTTTCAATACCGTGTACTACAAGATATATGCGTCTGCGAAAAGGGGAGGTCCAGCGAGTGGAGCATTGTTCTGGCAACTTCTTACAGAAGGAATGGAATCTTTCGACGATGGCTATGGAATATCGCTTGGTCAAAGTACTTCTACTGCTAATGTCATTGCTCAGCAATCTCATAAACTATACCTTATTCGGAAGATTTATGCAAAGATCGCTAATGAACGACGATGGAAAAGAGCTAGATTTAACGGAAGAAGGTCTTCACGCGTTGGTGGAAATAGAGGAAGACAAATTGGTTTCTGA

Protein sequence

>Msa0716560

MMKHFALLFLLAILVHQECFNINVEAAGDGFVRTRGIHFMLNGNPYYANGFNAYWLMYTASDPSQRFKVSNAFREATSHGLTVARTWAFSDGGYSPLQYSPGFYNEQMFKGLDFVISEARKHGIKLILSLVNNYESFGGKKQYVNWARSNNGQYLTSDDDFFRNPVVKGFYINHVKTVLNRYNSFTGIHYKDDPTIMAWELMNEPRCTSDPSGRTIQGWITEMASLVKSIDRNHLLEAGLEGFYGQSTPQRTKFNPTINNIGTDFIANNRIPGIDFATVHCYPDQWVQSSNEQDQLAFLNNWLSAHFLDAQYALKKPILVAEFGKSFKDSGFSSNQRDQLFNTVYYKIYASAKRGGPASGALFWQLLTEGMESFDDGYGISLGQSTSTANVIAQQSHKLYLIRKIYAKIANERRWKRARFNGRRSSRVGGNRGRQIGF*