AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0728440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATAATATTTGAACCTATT+TGG 0.165996 5_1:-33075447 Msa0728440:three_prime_UTR
GGGTTACATGTTTGATAAAT+TGG 0.217858 5_1:-33075507 Msa0728440:three_prime_UTR
GTTGGATTTAGATGATAAAT+AGG 0.238893 5_1:+33075708 None:intergenic
TTACAATGGTAGTTGTCTTT+TGG 0.253249 5_1:-33078411 Msa0728440:CDS
AACCAAATACAAAACTAAAT+AGG 0.295677 5_1:+33075296 None:intergenic
TACCGAATTTAAAACCAAAT+AGG 0.323650 5_1:+33075433 None:intergenic
AGAGTTGGATCTTTCAAGTT+TGG 0.344503 5_1:-33076760 Msa0728440:CDS
AACCGTGAGTGTAACTAAAT+AGG 0.356359 5_1:+33076401 None:intergenic
GCATTGTCTTCCTACATTGT+TGG 0.387969 5_1:+33075690 None:intergenic
TAGGAAGACAATGCATCAAA+TGG 0.400920 5_1:-33075681 Msa0728440:three_prime_UTR
ATACAAAGCTTCTTTATTGA+CGG 0.409686 5_1:-33075652 Msa0728440:three_prime_UTR
GATAAATTGGAAGTTTGAAC+TGG 0.414196 5_1:-33075494 Msa0728440:three_prime_UTR
TTCAATATGCAGTCTTATCA+AGG 0.421472 5_1:-33075621 Msa0728440:three_prime_UTR
AAATATTATGTTATGCATCA+AGG 0.435058 5_1:+33075459 None:intergenic
AAGAGAGTCACTAAAATTCA+AGG 0.442926 5_1:+33075865 None:intergenic
ATCATACAAAAGCAAATTCA+AGG 0.445622 5_1:-33076328 Msa0728440:three_prime_UTR
TAATAATTACTAATGTAGTA+AGG 0.472023 5_1:-33075782 Msa0728440:three_prime_UTR
CACGGTTATAGTCACATAAG+AGG 0.495209 5_1:-33076385 Msa0728440:three_prime_UTR
ACATAAGAGGAAGCAAGAGC+TGG 0.497961 5_1:-33076372 Msa0728440:three_prime_UTR
TAGCATCAAACCTTAATCAA+TGG 0.502082 5_1:+33076287 None:intergenic
AAAGATCCAACTCTAACTCT+TGG 0.506986 5_1:+33076769 None:intergenic
AGGAAGACAATGCATCAAAT+GGG 0.527537 5_1:-33075680 Msa0728440:three_prime_UTR
TGATAGCCAAGAGTTAGAGT+TGG 0.528815 5_1:-33076775 Msa0728440:CDS
AATATTATGTTATGCATCAA+GGG 0.534580 5_1:+33075460 None:intergenic
TCATCTAAATCCAACAATGT+AGG 0.539766 5_1:-33075700 Msa0728440:three_prime_UTR
TTCCTATTTAGTTACACTCA+CGG 0.574912 5_1:-33076403 Msa0728440:exon
TGAGTAGTGACGATGAGTAG+TGG 0.638549 5_1:-33078446 None:intergenic
ACAACTACCATTGTAAACAC+AGG 0.647981 5_1:+33078418 None:intergenic
GGTGGATCCTGTGTTTACAA+TGG 0.680380 5_1:-33078425 Msa0728440:CDS
TTATGCATCAAGGGACATCG+AGG 0.697162 5_1:+33075469 None:intergenic
TATGCATCAAGGGACATCGA+GGG 0.701146 5_1:+33075470 None:intergenic
GTAGTGACGATGAGTAGTGG+TGG 0.708051 5_1:-33078443 None:intergenic
AACACATTATGTTCACACAG+AGG 0.752547 5_1:+33075929 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTTTTTTTTTTTTTGAAA+CGG + chr5_1:33075480-33075499 None:intergenic 10.0%
!!! ATACAAAACAAAATAATTTT+TGG + chr5_1:33075749-33075768 None:intergenic 10.0%
!!! AAATTATTTTGTTTTGTATT+CGG - chr5_1:33075750-33075769 Msa0728440:three_prime_UTR 10.0%
!!! TATCAATTTTATAATAAACT+AGG + chr5_1:33076454-33076473 None:intergenic 10.0%
!!! TTATGAATGATATTTTTTAA+TGG - chr5_1:33077724-33077743 Msa0728440:intron 10.0%
!!! TATCAAACTTTTAAACATAT+AGG + chr5_1:33075824-33075843 None:intergenic 15.0%
!!! AACTAATTTCGTTTTAAATT+TGG - chr5_1:33075900-33075919 Msa0728440:three_prime_UTR 15.0%
!!! AACCTATTTTGTTTTAAATT+TGG - chr5_1:33076197-33076216 Msa0728440:intron 15.0%
!!! TACCAAATTTAAAACAAAAT+AGG + chr5_1:33076202-33076221 None:intergenic 15.0%
!!! AAATGCTAAAATTTCATTTT+CGG + chr5_1:33076279-33076298 None:intergenic 15.0%
!!! AAATGAAATTTTAGCATTTA+TGG - chr5_1:33076280-33076299 Msa0728440:intron 15.0%
!!! TTCAAAGTACTTTTAAATTT+GGG + chr5_1:33077548-33077567 None:intergenic 15.0%
!!! ATTCAAAGTACTTTTAAATT+TGG + chr5_1:33077549-33077568 None:intergenic 15.0%
!!! TCAAAATTTTAAACTTATTC+AGG + chr5_1:33077661-33077680 None:intergenic 15.0%
!!! GTTTAAAATTTTGAATAAGT+AGG - chr5_1:33077667-33077686 Msa0728440:intron 15.0%
!! TAATGTTCAAATTTCAAAAA+AGG + chr5_1:33077904-33077923 None:intergenic 15.0%
!! TAATAATTACTAATGTAGTA+AGG - chr5_1:33077932-33077951 Msa0728440:intron 15.0%
!!! ATGGTTACAAATTTTATAAA+TGG - chr5_1:33078355-33078374 Msa0728440:intron 15.0%
!!! TACAAATTTTATAAATGGAA+AGG - chr5_1:33078360-33078379 Msa0728440:intron 15.0%
!!! AACCTATTTGGTTTTATATT+CGG - chr5_1:33075602-33075621 Msa0728440:three_prime_UTR 20.0%
!! AAAATTATGTTACGCAATAA+TGG + chr5_1:33075878-33075897 None:intergenic 20.0%
!!! AACATATTTGGTTTGAAATT+CGG - chr5_1:33076049-33076068 Msa0728440:intron 20.0%
!! AATATCATGTTTCACATAAA+GGG + chr5_1:33076175-33076194 None:intergenic 20.0%
!! AAATATCATGTTTCACATAA+AGG + chr5_1:33076176-33076195 None:intergenic 20.0%
!!! AATGCTAAAATTTCATTTTC+GGG + chr5_1:33076278-33076297 None:intergenic 20.0%
!! TACAAAAATGATAAATGAGA+AGG - chr5_1:33076304-33076323 Msa0728440:three_prime_UTR 20.0%
!! ACAAAAATGATAAATGAGAA+GGG - chr5_1:33076305-33076324 Msa0728440:three_prime_UTR 20.0%
!!! AGACGTTTTAAACTATTAAT+TGG - chr5_1:33076535-33076554 Msa0728440:intron 20.0%
!! ATAGTTAGGAATTTGATAAA+TGG - chr5_1:33076628-33076647 Msa0728440:intron 20.0%
!! TAGTTAGGAATTTGATAAAT+GGG - chr5_1:33076629-33076648 Msa0728440:intron 20.0%
!! AGCATAGTTAATTCTAAATT+CGG - chr5_1:33076701-33076720 Msa0728440:intron 20.0%
!! TAATTCTAAATTCGGTAAAT+TGG - chr5_1:33076709-33076728 Msa0728440:intron 20.0%
!! AAAAACAAAAATCTTGAGAT+CGG + chr5_1:33077468-33077487 None:intergenic 20.0%
!!! TCATGTTATTTATCTACTTT+GGG - chr5_1:33077584-33077603 Msa0728440:intron 20.0%
!! AATATTATGTTATGCATCAA+GGG + chr5_1:33078257-33078276 None:intergenic 20.0%
!! AAATATTATGTTATGCATCA+AGG + chr5_1:33078258-33078277 None:intergenic 20.0%
!! ACATAATATTTGAACCTATT+TGG - chr5_1:33078267-33078286 Msa0728440:intron 20.0%
!!! AACCTATTTGGTTTTAAATT+CGG - chr5_1:33078279-33078298 Msa0728440:intron 20.0%
!!! AAATGCAATTTTAGCTTATA+TGG - chr5_1:33078336-33078355 Msa0728440:intron 20.0%
!!! TACATCATGTTTTAAATCAA+GGG + chr5_1:33078396-33078415 None:intergenic 20.0%
!!! TTACATCATGTTTTAAATCA+AGG + chr5_1:33078397-33078416 None:intergenic 20.0%
!!! AACCTATTTAGTTTTGTATT+TGG - chr5_1:33078416-33078435 Msa0728440:CDS 20.0%
!! AACCAAATACAAAACTAAAT+AGG + chr5_1:33078421-33078440 None:intergenic 20.0%
!! AAATGAAATGTTAGCCTATT+TGG - chr5_1:33075516-33075535 Msa0728440:three_prime_UTR 25.0%
! TATCAAACTTCTAACCAAAT+AGG + chr5_1:33075533-33075552 None:intergenic 25.0%
! TTCCGAATATAAAACCAAAT+AGG + chr5_1:33075607-33075626 None:intergenic 25.0%
!! ATTCGGAAAAATGAAAGTAT+TGG - chr5_1:33075619-33075638 Msa0728440:three_prime_UTR 25.0%
!!! AAATGAGATTTTAGCCTATA+TGG - chr5_1:33075658-33075677 Msa0728440:three_prime_UTR 25.0%
! TATCAAACTTCTAACCATAT+AGG + chr5_1:33075675-33075694 None:intergenic 25.0%
! TATGGTTAGAAGTTTGATAA+AGG - chr5_1:33075676-33075695 Msa0728440:three_prime_UTR 25.0%
!!! TAGGCTAAAATCTCATTTTT+GGG + chr5_1:33075805-33075824 None:intergenic 25.0%
!! ATAGGCTAAAATCTCATTTT+TGG + chr5_1:33075657-33075676 None:intergenic 25.0%
!! TACATGTTTGTTGAATATGA+AGG - chr5_1:33075981-33076000 Msa0728440:intron 25.0%
! ACATGATATGTGAACATATT+TGG - chr5_1:33076037-33076056 Msa0728440:intron 25.0%
!!! AAATGAAATTTTAGGCATTG+TGG - chr5_1:33076106-33076125 Msa0728440:intron 25.0%
!!! ATTTTTAAGCCAAGTTTGTT+TGG - chr5_1:33076365-33076384 Msa0728440:three_prime_UTR 25.0%
!!! ACTAGGTTCAAATCATTTTT+GGG + chr5_1:33076437-33076456 None:intergenic 25.0%
!! AACTAGGTTCAAATCATTTT+TGG + chr5_1:33076438-33076457 None:intergenic 25.0%
!! ACTTGATATTTAAGACTAGT+TGG - chr5_1:33076807-33076826 Msa0728440:intron 25.0%
!!! AGACTAGTTGGTTTTAAATT+CGG - chr5_1:33076819-33076838 Msa0728440:intron 25.0%
!! GCAGATAAAGTTTTCTTAAT+CGG + chr5_1:33077115-33077134 None:intergenic 25.0%
! CCAATTTATTGATCTCTTTA+CGG - chr5_1:33077225-33077244 Msa0728440:intron 25.0%
! ATCATACAAAAGCAAATTCA+AGG - chr5_1:33077386-33077405 Msa0728440:intron 25.0%
!!! TAATTTTCTGCCATTGATTA+AGG - chr5_1:33077417-33077436 Msa0728440:intron 25.0%
!! GTCATGTTATTTATCTACTT+TGG - chr5_1:33077583-33077602 Msa0728440:intron 25.0%
! ATATGAAATTGCTCAAAGTT+GGG + chr5_1:33077636-33077655 None:intergenic 25.0%
! TCTAACATACTTATATTAGC+AGG + chr5_1:33077703-33077722 None:intergenic 25.0%
!! ATGTGTTTTTCTTTCAACTA+AGG - chr5_1:33077801-33077820 Msa0728440:intron 25.0%
! TAGTAAGGTTGTGTAAAAAA+TGG - chr5_1:33077947-33077966 Msa0728440:intron 25.0%
! AGTAAGGTTGTGTAAAAAAT+GGG - chr5_1:33077948-33077967 Msa0728440:intron 25.0%
! GTTGGATTTAGATGATAAAT+AGG + chr5_1:33078009-33078028 None:intergenic 25.0%
! ATACAAAGCTTCTTTATTGA+CGG - chr5_1:33078062-33078081 Msa0728440:intron 25.0%
!! AAGCTAAAATCTCATTTTCT+CGG + chr5_1:33078182-33078201 None:intergenic 25.0%
!!! AATGAGATTTTAGCTTATCT+CGG - chr5_1:33078186-33078205 Msa0728440:intron 25.0%
!! TACCGAATTTAAAACCAAAT+AGG + chr5_1:33078284-33078303 None:intergenic 25.0%
! TTACAATGGTAGTTGTCTTT+TGG - chr5_1:33075303-33075322 Msa0728440:three_prime_UTR 30.0%
GAACGAAATCAAGATACAAA+TGG + chr5_1:33075416-33075435 None:intergenic 30.0%
ACATGATATGTGAACCTATT+TGG - chr5_1:33075590-33075609 Msa0728440:three_prime_UTR 30.0%
CATATCATGTTTCGTATCAA+GGG + chr5_1:33075722-33075741 None:intergenic 30.0%
ACATATCATGTTTCGTATCA+AGG + chr5_1:33075723-33075742 None:intergenic 30.0%
!!! AAATCTCATTTTTGGGACAA+CGG + chr5_1:33075798-33075817 None:intergenic 30.0%
! AAATTTCATTTTCGCGACAA+GGG + chr5_1:33076097-33076116 None:intergenic 30.0%
! AAAATTTCATTTTCGCGACA+AGG + chr5_1:33076098-33076117 None:intergenic 30.0%
! TGTCGCGAAAATGAAATTTT+AGG - chr5_1:33076098-33076117 Msa0728440:intron 30.0%
TGGTTGCAAGTTTGATAAAT+GGG - chr5_1:33076126-33076145 Msa0728440:intron 30.0%
AAAATCATGTTTCGCATCAA+GGG + chr5_1:33076350-33076369 None:intergenic 30.0%
AAAAATCATGTTTCGCATCA+AGG + chr5_1:33076351-33076370 None:intergenic 30.0%
!!! TTCAAATCATTTTTGGGTTG+AGG + chr5_1:33076431-33076450 None:intergenic 30.0%
!! GGATTTTAGTGTAGATAGTT+AGG - chr5_1:33076614-33076633 Msa0728440:intron 30.0%
TAGGAATTTGATAAATGGGA+AGG - chr5_1:33076633-33076652 Msa0728440:intron 30.0%
AAAAGGGTAAAGATGCAAAT+GGG + chr5_1:33077025-33077044 None:intergenic 30.0%
AAAAAGGGTAAAGATGCAAA+TGG + chr5_1:33077026-33077045 None:intergenic 30.0%
ACGCAGTCAAAAAGAAAAAA+GGG + chr5_1:33077041-33077060 None:intergenic 30.0%
TACGCAGTCAAAAAGAAAAA+AGG + chr5_1:33077042-33077061 None:intergenic 30.0%
CCGTAAAGAGATCAATAAAT+TGG + chr5_1:33077228-33077247 None:intergenic 30.0%
TTCCTATTTAGTTACACTCA+CGG - chr5_1:33077311-33077330 Msa0728440:intron 30.0%
! TTTTGTATGATGATTGCACT+CGG + chr5_1:33077377-33077396 None:intergenic 30.0%
TAGCATCAAACCTTAATCAA+TGG + chr5_1:33077430-33077449 None:intergenic 30.0%
TATGAAATTGCTCAAAGTTG+GGG + chr5_1:33077635-33077654 None:intergenic 30.0%
CATATGAAATTGCTCAAAGT+TGG + chr5_1:33077637-33077656 None:intergenic 30.0%
ATACTTATATTAGCAGGACA+TGG + chr5_1:33077697-33077716 None:intergenic 30.0%
TGTTGAATAATCATCTTTCC+AGG - chr5_1:33077747-33077766 Msa0728440:intron 30.0%
AAGAGAGTCACTAAAATTCA+AGG + chr5_1:33077852-33077871 None:intergenic 30.0%
TCATCTAAATCCAACAATGT+AGG - chr5_1:33078014-33078033 Msa0728440:intron 30.0%
! TTCAATATGCAGTCTTATCA+AGG - chr5_1:33078093-33078112 Msa0728440:intron 30.0%
!!! ATGAGATTTTAGCTTATCTC+GGG - chr5_1:33078187-33078206 Msa0728440:intron 30.0%
GGGTTACATGTTTGATAAAT+TGG - chr5_1:33078207-33078226 Msa0728440:intron 30.0%
GATAAATTGGAAGTTTGAAC+TGG - chr5_1:33078220-33078239 Msa0728440:intron 30.0%
ACAACTACCATTGTAAACAC+AGG + chr5_1:33075299-33075318 None:intergenic 35.0%
!! TGTCTTTTGGAGGTTGTTTT+AGG - chr5_1:33075316-33075335 Msa0728440:three_prime_UTR 35.0%
TATGGTGTTTCGTCTATATG+CGG - chr5_1:33075388-33075407 Msa0728440:three_prime_UTR 35.0%
! ACATTTCATTTTCGCGACAA+GGG + chr5_1:33075507-33075526 None:intergenic 35.0%
! AACATTTCATTTTCGCGACA+AGG + chr5_1:33075508-33075527 None:intergenic 35.0%
CATATCATGTTTCGCATCAA+GGG + chr5_1:33075580-33075599 None:intergenic 35.0%
ACATATCATGTTTCGCATCA+AGG + chr5_1:33075581-33075600 None:intergenic 35.0%
! AAATCTCGTTTTCGCGATAA+GGG + chr5_1:33075948-33075967 None:intergenic 35.0%
! AAAATCTCGTTTTCGCGATA+AGG + chr5_1:33075949-33075968 None:intergenic 35.0%
CATATCATGTTTCGCATCAA+GGG + chr5_1:33075580-33075599 None:intergenic 35.0%
ACATATCATGTTTCGCATCA+AGG + chr5_1:33075581-33075600 None:intergenic 35.0%
GTGGTTGCAAGTTTGATAAA+TGG - chr5_1:33076125-33076144 Msa0728440:intron 35.0%
! AGCCAAGTTTGTTTGGATTT+CGG - chr5_1:33076372-33076391 Msa0728440:three_prime_UTR 35.0%
TACCGAAATCCAAACAAACT+TGG + chr5_1:33076377-33076396 None:intergenic 35.0%
TTGTTTGGATTTCGGTAAAC+TGG - chr5_1:33076380-33076399 Msa0728440:three_prime_UTR 35.0%
! AAATCCAATTTTCGCGTCAA+GGG + chr5_1:33076600-33076619 None:intergenic 35.0%
! AAAATCCAATTTTCGCGTCA+AGG + chr5_1:33076601-33076620 None:intergenic 35.0%
GTTCTCAAACTTCTAAACGA+GGG + chr5_1:33076759-33076778 None:intergenic 35.0%
GTTTAGAAGTTTGAGAACGA+AGG - chr5_1:33076761-33076780 Msa0728440:CDS 35.0%
TTTCGAAACAAGGGTCATTA+AGG + chr5_1:33076868-33076887 None:intergenic 35.0%
! AATGACCCTTGTTTCGAAAA+TGG - chr5_1:33076869-33076888 Msa0728440:intron 35.0%
! TCGATCCATTTTCGAAACAA+GGG + chr5_1:33076877-33076896 None:intergenic 35.0%
! ATCGATCCATTTTCGAAACA+AGG + chr5_1:33076878-33076897 None:intergenic 35.0%
TTTCGAAAATGGATCGATAG+TGG - chr5_1:33076880-33076899 Msa0728440:intron 35.0%
TATCAAATACAACAGCCTAC+AGG + chr5_1:33076924-33076943 None:intergenic 35.0%
AAAGATCCAACTCTAACTCT+TGG + chr5_1:33076948-33076967 None:intergenic 35.0%
!! AGAGTTGGATCTTTCAAGTT+TGG - chr5_1:33076954-33076973 Msa0728440:intron 35.0%
! CAATGATTGCTGCAGTTTTT+AGG - chr5_1:33076983-33077002 Msa0728440:intron 35.0%
TTCACATACGACAACAAACA+TGG - chr5_1:33077085-33077104 Msa0728440:intron 35.0%
! AACTTTATCTGCGACTGTTT+TGG - chr5_1:33077123-33077142 Msa0728440:intron 35.0%
TACAATTCGCCAACTTACAT+TGG - chr5_1:33077168-33077187 Msa0728440:intron 35.0%
ACAATTCGCCAACTTACATT+GGG - chr5_1:33077169-33077188 Msa0728440:intron 35.0%
! TACCTACCCTTTTAGATACA+AGG + chr5_1:33077198-33077217 None:intergenic 35.0%
AACCGTGAGTGTAACTAAAT+AGG + chr5_1:33077316-33077335 None:intergenic 35.0%
AGGACAAAATGATGTATACC+TGG + chr5_1:33077768-33077787 None:intergenic 35.0%
AACACATTATGTTCACACAG+AGG + chr5_1:33077788-33077807 None:intergenic 35.0%
AGGTTGTGTAAAAAATGGGA+AGG - chr5_1:33077952-33077971 Msa0728440:intron 35.0%
TAGGAAGACAATGCATCAAA+TGG - chr5_1:33078033-33078052 Msa0728440:intron 35.0%
AGGAAGACAATGCATCAAAT+GGG - chr5_1:33078034-33078053 Msa0728440:intron 35.0%
! AAATTGCATTTTCGCGACAA+GGG + chr5_1:33078327-33078346 None:intergenic 35.0%
! AAAATTGCATTTTCGCGACA+AGG + chr5_1:33078328-33078347 None:intergenic 35.0%
! CAATGGTAGTTGTCTTTTGG+AGG - chr5_1:33075306-33075325 Msa0728440:three_prime_UTR 40.0%
!!! CTTTTGGAGGTTGTTTTAGG+TGG - chr5_1:33075319-33075338 Msa0728440:three_prime_UTR 40.0%
TGTTGGAGAGTTTATCTCCA+GGG - chr5_1:33075349-33075368 Msa0728440:three_prime_UTR 40.0%
!!! TATCTCCAGGGGTGTTTTTT+AGG - chr5_1:33075361-33075380 Msa0728440:three_prime_UTR 40.0%
!!! ATCTCCAGGGGTGTTTTTTA+GGG - chr5_1:33075362-33075381 Msa0728440:three_prime_UTR 40.0%
!! TACGCAATAATGGACATCGA+GGG + chr5_1:33075868-33075887 None:intergenic 40.0%
!! TTACGCAATAATGGACATCG+AGG + chr5_1:33075869-33075888 None:intergenic 40.0%
TTTCACATAAAGGGACCTGA+GGG + chr5_1:33076166-33076185 None:intergenic 40.0%
AAACTGGATGCAAGAGATTG+TGG - chr5_1:33076396-33076415 Msa0728440:exon 40.0%
TTCACATCAAGTGACATCGA+GGG + chr5_1:33076669-33076688 None:intergenic 40.0%
TTTCACATCAAGTGACATCG+AGG + chr5_1:33076670-33076689 None:intergenic 40.0%
CTTCTAAACGAGGGTCATAA+AGG + chr5_1:33076750-33076769 None:intergenic 40.0%
CGTTCTCAAACTTCTAAACG+AGG + chr5_1:33076760-33076779 None:intergenic 40.0%
AATATTTCACGCTTGCCTGT+AGG - chr5_1:33076906-33076925 Msa0728440:intron 40.0%
TGATAGCCAAGAGTTAGAGT+TGG - chr5_1:33076939-33076958 Msa0728440:intron 40.0%
!!! GTTTTGGAAGAGACACTCAA+TGG - chr5_1:33077139-33077158 Msa0728440:intron 40.0%
TGGGACACCTTGTATCTAAA+AGG - chr5_1:33077188-33077207 Msa0728440:intron 40.0%
GGGACACCTTGTATCTAAAA+GGG - chr5_1:33077189-33077208 Msa0728440:intron 40.0%
CACCTTGTATCTAAAAGGGT+AGG - chr5_1:33077193-33077212 Msa0728440:intron 40.0%
CACGGTTATAGTCACATAAG+AGG - chr5_1:33077329-33077348 Msa0728440:intron 40.0%
GCATTGTCTTCCTACATTGT+TGG + chr5_1:33078027-33078046 None:intergenic 40.0%
GGTGGATCCTGTGTTTACAA+TGG - chr5_1:33075289-33075308 Msa0728440:three_prime_UTR 45.0%
!! TTTTAGGTGGTGAGGTGTGT+TGG - chr5_1:33075332-33075351 Msa0728440:three_prime_UTR 45.0%
GTGTTGGAGAGTTTATCTCC+AGG - chr5_1:33075348-33075367 Msa0728440:three_prime_UTR 45.0%
GTTGGAGAGTTTATCTCCAG+GGG - chr5_1:33075350-33075369 Msa0728440:three_prime_UTR 45.0%
!!! TCTCCAGGGGTGTTTTTTAG+GGG - chr5_1:33075363-33075382 Msa0728440:three_prime_UTR 45.0%
!!! GGGTGTTTTTTAGGGGGTTA+TGG - chr5_1:33075370-33075389 Msa0728440:three_prime_UTR 45.0%
TTCGTATCAAGGGAGATCGA+GGG + chr5_1:33075712-33075731 None:intergenic 45.0%
TTTCGTATCAAGGGAGATCG+AGG + chr5_1:33075713-33075732 None:intergenic 45.0%
GAAGTTTGAACTTGTCCCTC+AGG - chr5_1:33076148-33076167 Msa0728440:intron 45.0%
GTTTCACATAAAGGGACCTG+AGG + chr5_1:33076167-33076186 None:intergenic 45.0%
TATGCCCTTGACGCGAAAAT+TGG - chr5_1:33076593-33076612 Msa0728440:intron 45.0%
! CAAGGTGTCCCAATGTAAGT+TGG + chr5_1:33077180-33077199 None:intergenic 45.0%
!! CTTTGCTGCTTTCATGTCAC+AGG - chr5_1:33077269-33077288 Msa0728440:intron 45.0%
ACATAAGAGGAAGCAAGAGC+TGG - chr5_1:33077342-33077361 Msa0728440:intron 45.0%
TATGCATCAAGGGACATCGA+GGG + chr5_1:33078247-33078266 None:intergenic 45.0%
TTATGCATCAAGGGACATCG+AGG + chr5_1:33078248-33078267 None:intergenic 45.0%
!! TTATTATAAAATTGATAAAT+AGG - chr5_1:33076457-33076476 Msa0728440:intron 5.0%
!!! GGAGGTTGTTTTAGGTGGTG+AGG - chr5_1:33075324-33075343 Msa0728440:three_prime_UTR 50.0%
!!! CTCCAGGGGTGTTTTTTAGG+GGG - chr5_1:33075364-33075383 Msa0728440:three_prime_UTR 50.0%
AACCCCCTAAAAAACACCCC+TGG + chr5_1:33075369-33075388 None:intergenic 50.0%
TTCGCATCAAGGGACATCGA+GGG + chr5_1:33075570-33075589 None:intergenic 50.0%
TTTCGCATCAAGGGACATCG+AGG + chr5_1:33075571-33075590 None:intergenic 50.0%
TTCGCATCAAGGGACATCGA+TGG + chr5_1:33075570-33075589 None:intergenic 50.0%
TTTCGCATCAAGGGACATCG+AGG + chr5_1:33075571-33075590 None:intergenic 50.0%
CGTTCACTCCTTGCGTCTTG+CGG - chr5_1:33075431-33075450 Msa0728440:three_prime_UTR 55.0%
GTTCACTCCTTGCGTCTTGC+GGG - chr5_1:33075432-33075451 Msa0728440:three_prime_UTR 55.0%
TTCACTCCTTGCGTCTTGCG+GGG - chr5_1:33075433-33075452 Msa0728440:three_prime_UTR 55.0%
CGTCATCCCCGCAAGACGCA+AGG + chr5_1:33075442-33075461 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 33075280 33078456 33075280 ID=Msa0728440;Name=Msa0728440
chr5_1 mRNA 33075280 33078456 33075280 ID=Msa0728440-mRNA-1;Parent=Msa0728440;Name=Msa0728440-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|0.33|0.75|0.75|0.66|0.5|4|805|57
chr5_1 exon 33075280 33075967 33075280 ID=Msa0728440-mRNA-1:exon:10635;Parent=Msa0728440-mRNA-1
chr5_1 exon 33076298 33076445 33076298 ID=Msa0728440-mRNA-1:exon:10634;Parent=Msa0728440-mRNA-1
chr5_1 exon 33076732 33076808 33076732 ID=Msa0728440-mRNA-1:exon:10633;Parent=Msa0728440-mRNA-1
chr5_1 exon 33078391 33078456 33078391 ID=Msa0728440-mRNA-1:exon:10632;Parent=Msa0728440-mRNA-1
chr5_1 CDS 33078391 33078456 33078391 ID=Msa0728440-mRNA-1:cds;Parent=Msa0728440-mRNA-1
chr5_1 CDS 33076732 33076808 33076732 ID=Msa0728440-mRNA-1:cds;Parent=Msa0728440-mRNA-1
chr5_1 CDS 33076415 33076445 33076415 ID=Msa0728440-mRNA-1:cds;Parent=Msa0728440-mRNA-1
chr5_1 three_prime_UTR 33076298 33076414 33076298 ID=Msa0728440-mRNA-1:three_prime_utr;Parent=Msa0728440-mRNA-1
chr5_1 three_prime_UTR 33075280 33075967 33075280 ID=Msa0728440-mRNA-1:three_prime_utr;Parent=Msa0728440-mRNA-1
Gene Sequence

>Msa0728440

ATGAGTAGTGGTGGATCCTGTGTTTACAATGGTAGTTGTCTTTTGGAGGTTGTTTTAGGTGGTGAGGCTGTTGTATTTGATAGCCAAGAGTTAGAGTTGGATCTTTCAAGTTTGGATGCTACAATGATTGCTGCAGTTTTTAGGTTGAGAATAAATGTGCACATTCCTATTTAG
Protein sequence

>Msa0728440

MSSGGSCVYNGSCLLEVVLGGEAVVFDSQELELDLSSLDATMIAAVFRLRINVHIPI*