Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0010770 | Msa0798640 | 0.814429 | 1.547791e-51 | -8.615850e-47 |
| Msa0022550 | Msa0798640 | 0.803988 | 2.680383e-49 | -8.615850e-47 |
| Msa0022750 | Msa0798640 | -0.815584 | 8.579013e-52 | -8.615850e-47 |
| Msa0039250 | Msa0798640 | 0.811766 | 5.940878e-51 | -8.615850e-47 |
| Msa0074750 | Msa0798640 | 0.810804 | 9.609259e-51 | -8.615850e-47 |
| Msa0096580 | Msa0798640 | 0.800769 | 1.233832e-48 | -8.615850e-47 |
| Msa0097950 | Msa0798640 | 0.801094 | 1.058893e-48 | -8.615850e-47 |
| Msa0606810 | Msa0798640 | 0.823390 | 1.420984e-53 | -8.615850e-47 |
| Msa0628500 | Msa0798640 | 0.826163 | 3.154597e-54 | -8.615850e-47 |
| Msa0640940 | Msa0798640 | 0.802722 | 4.902863e-49 | -8.615850e-47 |
| Msa0652690 | Msa0798640 | 0.811053 | 8.483801e-51 | -8.615850e-47 |
| Msa0673270 | Msa0798640 | 0.807160 | 5.788236e-50 | -8.615850e-47 |
| Msa0679360 | Msa0798640 | 0.803571 | 3.271934e-49 | -8.615850e-47 |
| Msa0686080 | Msa0798640 | 0.801538 | 8.590753e-49 | -8.615850e-47 |
| Msa0689760 | Msa0798640 | 0.815482 | 9.039384e-52 | -8.615850e-47 |
| Msa0710270 | Msa0798640 | 0.809283 | 2.042288e-50 | -8.615850e-47 |
| Msa0716860 | Msa0798640 | 0.805923 | 1.056083e-49 | -8.615850e-47 |
| Msa0726080 | Msa0798640 | 0.800698 | 1.275917e-48 | -8.615850e-47 |
| Msa0743530 | Msa0798640 | 0.808004 | 3.831590e-50 | -8.615850e-47 |
| Msa0750250 | Msa0798640 | 0.814992 | 1.161502e-51 | -8.615850e-47 |
| Msa0750330 | Msa0798640 | -0.800283 | 1.549683e-48 | -8.615850e-47 |
| Msa0798640 | Msa0811040 | 0.807059 | 6.081274e-50 | -8.615850e-47 |
| Msa0798640 | Msa0820890 | 0.802057 | 6.721760e-49 | -8.615850e-47 |
| Msa0798640 | Msa0846230 | 0.800018 | 1.754557e-48 | -8.615850e-47 |
| Msa0798640 | Msa0866400 | 0.826538 | 2.567376e-54 | -8.615850e-47 |
| Msa0798640 | Msa0866410 | 0.814616 | 1.406864e-51 | -8.615850e-47 |
| Msa0798640 | Msa0919470 | 0.805463 | 1.318894e-49 | -8.615850e-47 |
| Msa0798640 | Msa0945240 | 0.810406 | 1.171080e-50 | -8.615850e-47 |
| Msa0798640 | Msa0957870 | 0.813235 | 2.836165e-51 | -8.615850e-47 |
| Msa0798640 | Msa0957970 | 0.810254 | 1.262904e-50 | -8.615850e-47 |
| Msa0798640 | Msa1010070 | 0.801954 | 7.057152e-49 | -8.615850e-47 |
| Msa0798640 | Msa1010460 | 0.815121 | 1.087258e-51 | -8.615850e-47 |
| Msa0798640 | Msa1127430 | 0.804935 | 1.701394e-49 | -8.615850e-47 |
| Msa0798640 | Msa1128330 | 0.816689 | 4.858510e-52 | -8.615850e-47 |
| Msa0798640 | Msa1168550 | 0.804626 | 1.973480e-49 | -8.615850e-47 |
| Msa0798640 | Msa1206650 | 0.807576 | 4.725159e-50 | -8.615850e-47 |
| Msa0798640 | Msa1211340 | 0.810784 | 9.701232e-51 | -8.615850e-47 |
| Msa0798640 | Msa1214320 | 0.813324 | 2.711972e-51 | -8.615850e-47 |
| Msa0798640 | Msa1230040 | 0.801448 | 8.960212e-49 | -8.615850e-47 |
| Msa0798640 | Msa1250110 | 0.819756 | 9.829139e-53 | -8.615850e-47 |
| Msa0798640 | Msa1250730 | 0.815219 | 1.033938e-51 | -8.615850e-47 |
| Msa0798640 | Msa1259640 | 0.807507 | 4.886817e-50 | -8.615850e-47 |
| Msa0798640 | Msa1272600 | 0.801916 | 7.183533e-49 | -8.615850e-47 |
| Msa0798640 | Msa1297530 | 0.801567 | 8.472676e-49 | -8.615850e-47 |
| Msa0798640 | Msa1299330 | 0.822980 | 1.771968e-53 | -8.615850e-47 |
| Msa0798640 | Msa1320820 | 0.823154 | 1.613333e-53 | -8.615850e-47 |
| Msa0798640 | Msa1329790 | 0.803745 | 3.010002e-49 | -8.615850e-47 |
| Msa0798640 | Msa1372290 | 0.811081 | 8.365908e-51 | -8.615850e-47 |
| Msa0798640 | Msa1400120 | 0.809911 | 1.497089e-50 | -8.615850e-47 |
| Msa0798640 | Msa1416260 | 0.815076 | 1.112342e-51 | -8.615850e-47 |
| Msa0798640 | Msa1420250 | 0.816837 | 4.501302e-52 | -8.615850e-47 |
| Msa0798640 | Msa1442790 | 0.802490 | 5.473939e-49 | -8.615850e-47 |
| Msa0133720 | Msa0798640 | 0.826150 | 3.176518e-54 | -8.615850e-47 |
| Msa0133820 | Msa0798640 | 0.808702 | 2.720719e-50 | -8.615850e-47 |
| Msa0136120 | Msa0798640 | 0.800276 | 1.555247e-48 | -8.615850e-47 |
| Msa0138210 | Msa0798640 | 0.811296 | 7.517445e-51 | -8.615850e-47 |
| Msa0142500 | Msa0798640 | 0.814683 | 1.359893e-51 | -8.615850e-47 |
| Msa0146080 | Msa0798640 | 0.812239 | 4.685896e-51 | -8.615850e-47 |
| Msa0183420 | Msa0798640 | 0.814114 | 1.816643e-51 | -8.615850e-47 |
| Msa0207030 | Msa0798640 | 0.805977 | 1.028424e-49 | -8.615850e-47 |
| Msa0227230 | Msa0798640 | 0.807700 | 4.447934e-50 | -8.615850e-47 |
| Msa0237200 | Msa0798640 | 0.814815 | 1.271171e-51 | -8.615850e-47 |
| Msa0381000 | Msa0798640 | 0.807853 | 4.127125e-50 | -8.615850e-47 |
| Msa0381080 | Msa0798640 | 0.805130 | 1.548860e-49 | -8.615850e-47 |
| Msa0394690 | Msa0798640 | 0.822080 | 2.868478e-53 | -8.615850e-47 |
| Msa0395980 | Msa0798640 | 0.812306 | 4.529454e-51 | -8.615850e-47 |
| Msa0397820 | Msa0798640 | 0.800543 | 1.371649e-48 | -8.615850e-47 |
| Msa0443430 | Msa0798640 | 0.829529 | 4.891175e-55 | -8.615850e-47 |
| Msa0473980 | Msa0798640 | 0.801974 | 6.991200e-49 | -8.615850e-47 |
| Msa0480780 | Msa0798640 | 0.811185 | 7.945631e-51 | -8.615850e-47 |
| Msa0274970 | Msa0798640 | 0.803488 | 3.403964e-49 | -8.615850e-47 |
| Msa0285330 | Msa0798640 | 0.812814 | 3.508445e-51 | -8.615850e-47 |
| Msa0310430 | Msa0798640 | 0.806190 | 9.276277e-50 | -8.615850e-47 |
| Msa0315680 | Msa0798640 | 0.803312 | 3.701971e-49 | -8.615850e-47 |
| Msa0324240 | Msa0798640 | 0.804153 | 2.476175e-49 | -8.615850e-47 |
| Msa0347950 | Msa0798640 | 0.825017 | 5.895763e-54 | -8.615850e-47 |
| Msa0347990 | Msa0798640 | 0.831559 | 1.558861e-55 | -8.615850e-47 |
| Msa0489660 | Msa0798640 | 0.821569 | 3.766061e-53 | -8.615850e-47 |
| Msa0491000 | Msa0798640 | 0.813895 | 2.030617e-51 | -8.615850e-47 |
| Msa0566350 | Msa0798640 | 0.800110 | 1.680779e-48 | -8.615850e-47 |
| Msa0566750 | Msa0798640 | 0.811465 | 6.906547e-51 | -8.615850e-47 |
| Msa0573040 | Msa0798640 | 0.812962 | 3.256262e-51 | -8.615850e-47 |
| Msa0577200 | Msa0798640 | -0.807809 | 4.215625e-50 | -8.615850e-47 |
| Msa0588120 | Msa0798640 | 0.817486 | 3.217044e-52 | -8.615850e-47 |
| Msa0600450 | Msa0798640 | 0.802448 | 5.584307e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 5 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGTACCTGTTAGATTTGC+AGG | 0.274237 | 5_3:-11387205 | None:intergenic |
| AAAGGAGAGAAAGAAACGAC+AGG | 0.473228 | 5_3:+11387054 | Msa0798640:CDS |
| AGAAAGAGATAGAGAAACTG+TGG | 0.508313 | 5_3:+11387117 | Msa0798640:CDS |
| TTAGATTTGCAGGGTATGAA+AGG | 0.536268 | 5_3:-11387195 | None:intergenic |
| ATGTACCTGTTAGATTTGCA+GGG | 0.596460 | 5_3:-11387204 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTATTAAATCAAAAGTAG+AGG | - | chr5_3:11386944-11386963 | None:intergenic | 15.0% |
| !! | AGAAACAAAAGAAAAAGAAA+AGG | + | chr5_3:11387036-11387055 | Msa0798640:CDS | 20.0% |
| !! | TTTCATTTTCATTTTCCAGA+AGG | + | chr5_3:11386983-11387002 | Msa0798640:intron | 25.0% |
| ! | ACAAATAGAGAGAAAAAGAA+AGG | + | chr5_3:11387082-11387101 | Msa0798640:CDS | 25.0% |
| ! | CAAATAGAGAGAAAAAGAAA+GGG | + | chr5_3:11387083-11387102 | Msa0798640:CDS | 25.0% |
| ! | AAATAGAGAGAAAAAGAAAG+GGG | + | chr5_3:11387084-11387103 | Msa0798640:CDS | 25.0% |
| ! | TATATTTTGCAATCACCTTC+TGG | - | chr5_3:11387001-11387020 | None:intergenic | 30.0% |
| !! | TTTTCTTTTGTTTCTCTTCG+TGG | - | chr5_3:11387031-11387050 | None:intergenic | 30.0% |
| AGAAAGAGATAGAGAAACTG+TGG | + | chr5_3:11387117-11387136 | Msa0798640:CDS | 35.0% | |
| TTAGATTTGCAGGGTATGAA+AGG | - | chr5_3:11387198-11387217 | None:intergenic | 35.0% | |
| AAAGGAGAGAAAGAAACGAC+AGG | + | chr5_3:11387054-11387073 | Msa0798640:CDS | 40.0% | |
| ! | CGTTTTCTCTTCTGTGGTTT+TGG | - | chr5_3:11387153-11387172 | None:intergenic | 40.0% |
| ! | AGACATCGTTTTCTCTTCTG+TGG | - | chr5_3:11387159-11387178 | None:intergenic | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 11386888 | 11387217 | 11386888 | ID=Msa0798640;Name=Msa0798640 |
| chr5_3 | mRNA | 11386888 | 11387217 | 11386888 | ID=Msa0798640-mRNA-1;Parent=Msa0798640;Name=Msa0798640-mRNA-1;_AED=0.57;_eAED=1.00;_QI=0|0|0|0.5|1|1|2|0|74 |
| chr5_3 | exon | 11386888 | 11386896 | 11386888 | ID=Msa0798640-mRNA-1:exon:4748;Parent=Msa0798640-mRNA-1 |
| chr5_3 | exon | 11387002 | 11387217 | 11387002 | ID=Msa0798640-mRNA-1:exon:4749;Parent=Msa0798640-mRNA-1 |
| chr5_3 | CDS | 11386888 | 11386896 | 11386888 | ID=Msa0798640-mRNA-1:cds;Parent=Msa0798640-mRNA-1 |
| chr5_3 | CDS | 11387002 | 11387217 | 11387002 | ID=Msa0798640-mRNA-1:cds;Parent=Msa0798640-mRNA-1 |
| Gene Sequence |
| Protein sequence |
