AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0997550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0997550 MtrunA17_Chr6g0483361 78.333 480 66 4 1 442 1 480 0.0 712
Msa0997550 MtrunA17_Chr6g0483381 77.178 482 70 5 1 442 1 482 0.0 706
Msa0997550 MtrunA17_Chr6g0477961 73.444 482 88 5 1 442 1 482 0.0 646
Msa0997550 MtrunA17_Chr6g0483351 78.177 417 35 4 82 442 18 434 0.0 599
Msa0997550 MtrunA17_Chr8g0392601 53.611 457 172 4 16 434 3 457 2.66e-166 476
Msa0997550 MtrunA17_Chr1g0208791 51.037 482 187 7 3 440 15 491 1.14e-162 468
Msa0997550 MtrunA17_Chr6g0483331 55.195 462 166 6 20 442 7 466 3.83e-162 465
Msa0997550 MtrunA17_Chr6g0483341 53.247 462 175 6 20 442 36 495 4.86e-162 466
Msa0997550 MtrunA17_Chr1g0208821 43.939 462 219 4 10 433 26 485 1.02e-122 366
Msa0997550 MtrunA17_Chr6g0449061 42.275 466 224 6 5 431 14 473 3.16e-118 354
Msa0997550 MtrunA17_Chr1g0208881 43.441 465 217 5 13 433 7 469 4.20e-114 343
Msa0997550 MtrunA17_Chr7g0251331 40.211 475 241 5 9 442 7 479 8.94e-114 342
Msa0997550 MtrunA17_Chr6g0483161 50.568 352 152 4 82 423 20 359 2.22e-111 332
Msa0997550 MtrunA17_Chr1g0208891 41.075 465 229 5 13 434 7 469 1.33e-108 329
Msa0997550 MtrunA17_Chr2g0317131 37.006 481 245 8 4 430 11 487 1.34e-94 293
Msa0997550 MtrunA17_Chr2g0317151 37.336 458 246 6 4 422 11 466 2.78e-92 287
Msa0997550 MtrunA17_Chr2g0322671 35.874 446 246 6 26 433 53 496 1.79e-89 281
Msa0997550 MtrunA17_Chr7g0251321 41.292 356 187 3 86 431 22 365 6.70e-88 272
Msa0997550 MtrunA17_Chr4g0024501 35.874 446 246 5 26 433 65 508 1.13e-87 276
Msa0997550 MtrunA17_Chr2g0317161 33.895 475 264 7 14 440 13 485 3.54e-84 266
Msa0997550 MtrunA17_Chr2g0317171 33.612 479 268 7 10 440 15 491 1.30e-81 260
Msa0997550 MtrunA17_Chr2g0288281 39.037 374 204 6 82 433 18 389 1.54e-81 256
Msa0997550 MtrunA17_Chr5g0442331 32.911 474 274 6 9 440 26 497 2.54e-76 246
Msa0997550 MtrunA17_Chr2g0317221 36.815 383 214 6 82 440 27 405 1.21e-74 239
Msa0997550 MtrunA17_Chr5g0412771 33.260 457 264 6 15 433 46 499 1.80e-74 242
Msa0997550 MtrunA17_Chr5g0428251 31.878 458 272 4 21 440 51 506 1.07e-73 241
Msa0997550 MtrunA17_Chr8g0368001 33.481 451 255 8 21 431 46 491 6.12e-73 238
Msa0997550 MtrunA17_Chr1g0203791 30.867 473 281 7 8 439 47 514 7.88e-72 236
Msa0997550 MtrunA17_Chr5g0397991 31.838 468 271 7 5 429 43 505 1.97e-70 232
Msa0997550 MtrunA17_Chr3g0140931 32.473 465 264 8 10 429 32 491 1.35e-69 230
Msa0997550 MtrunA17_Chr6g0483264 55.392 204 82 1 237 431 1 204 3.82e-67 214
Msa0997550 MtrunA17_Chr6g0473701 30.998 471 276 8 1 427 101 566 8.19e-66 221
Msa0997550 MtrunA17_Chr1g0154441 35.657 373 212 5 83 433 19 385 9.93e-65 213
Msa0997550 MtrunA17_Chr7g0254521 31.938 454 262 7 21 433 27 474 1.24e-64 215
Msa0997550 MtrunA17_Chr1g0202761 32.688 465 262 9 11 433 30 485 1.36e-64 216
Msa0997550 MtrunA17_Chr5g0412541 32.258 465 266 8 6 427 16 474 2.67e-64 214
Msa0997550 MtrunA17_Chr8g0389801 31.291 457 275 5 7 427 6 459 3.42e-64 214
Msa0997550 MtrunA17_Chr6g0467321 30.490 469 259 9 15 427 32 489 7.52e-64 214
Msa0997550 MtrunA17_Chr7g0254511 30.152 461 278 10 12 433 17 472 8.15e-64 213
Msa0997550 MtrunA17_Chr6g0468521 29.645 479 280 9 3 432 1 471 2.48e-60 204
Msa0997550 MtrunA17_Chr7g0254531 33.505 388 233 7 68 433 1 385 6.19e-60 201
Msa0997550 MtrunA17_Chr3g0132531 30.769 468 282 7 10 438 31 495 7.83e-60 203
Msa0997550 MtrunA17_Chr6g0468531 29.916 478 281 7 3 432 1 472 8.12e-60 202
Msa0997550 MtrunA17_Chr5g0412571 34.247 365 219 7 82 427 57 419 5.62e-56 191
Msa0997550 MtrunA17_Chr4g0013031 28.166 458 287 9 25 442 66 521 2.15e-54 189
Msa0997550 MtrunA17_Chr6g0468511 29.046 482 291 9 3 438 1 477 8.12e-54 186
Msa0997550 MtrunA17_Chr7g0248051 29.778 450 273 8 15 425 59 504 1.60e-52 184
Msa0997550 MtrunA17_Chr6g0483261 56.051 157 69 0 275 431 6 162 7.80e-51 170
Msa0997550 MtrunA17_Chr6g0483221 53.020 149 70 0 275 423 6 154 2.39e-50 168
Msa0997550 MtrunA17_Chr2g0295631 26.180 466 292 6 18 431 13 478 3.06e-49 175
Msa0997550 MtrunA17_Chr7g0254501 34.717 265 160 3 177 432 39 299 4.79e-49 169
Msa0997550 MtrunA17_Chr6g0460541 30.952 378 236 7 82 439 22 394 4.32e-46 164
Msa0997550 MtrunA17_Chr6g0487091 34.711 242 142 3 207 434 161 400 3.15e-39 146
Msa0997550 MtrunA17_Chr8g0364751 42.308 182 103 1 263 442 5 186 1.23e-38 138
Msa0997550 MtrunA17_Chr6g0483241 48.780 164 72 3 82 235 20 181 1.05e-36 132
Msa0997550 MtrunA17_Chr5g0440661 28.530 347 181 7 87 422 23 313 2.46e-35 133
Msa0997550 MtrunA17_Chr3g0132541 25.159 473 262 14 6 439 16 435 1.06e-30 122
Msa0997550 MtrunA17_Chr8g0364761 33.460 263 143 7 26 258 54 314 1.34e-30 120
Msa0997550 MtrunA17_Chr6g0460521 33.654 208 129 1 234 432 1 208 8.31e-29 113
Msa0997550 MtrunA17_Chr5g0398571 31.132 212 137 1 225 427 74 285 1.59e-25 106
Msa0997550 MtrunA17_Chr6g0483251 58.462 65 27 0 20 84 5 69 7.32e-18 77.8
Msa0997550 MtrunA17_Chr4g0025551 32.558 172 99 6 84 254 1 156 2.23e-16 76.3
Msa0997550 MtrunA17_Chr6g0483281 60.317 63 23 1 177 239 16 76 1.01e-15 72.4
Msa0997550 MtrunA17_Chr6g0483291 55.556 63 27 1 20 81 5 67 1.05e-15 71.6
Msa0997550 MtrunA17_Chr5g0442311 30.189 159 99 3 87 235 23 179 1.06e-13 69.3
Msa0997550 MtrunA17_Chr6g0483171 38.889 90 51 3 19 107 4 90 1.50e-11 61.2
Msa0997550 MtrunA17_Chr2g0317211 35.238 105 47 3 10 93 15 119 7.39e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0997550 AT5G52450.1 59.565 460 146 6 19 440 19 476 0.0 525
Msa0997550 AT2G34360.1 57.173 467 161 7 6 440 12 471 0.0 521
Msa0997550 AT1G73700.1 55.460 467 164 6 6 434 8 468 4.99e-170 486
Msa0997550 AT2G34360.2 50.862 464 153 6 6 440 12 429 3.62e-156 449
Msa0997550 AT1G71140.1 42.451 457 221 5 15 431 13 467 6.55e-120 358
Msa0997550 AT1G15170.1 40.667 450 227 5 26 437 34 481 2.77e-105 321
Msa0997550 AT1G15160.1 36.975 476 257 5 5 439 10 483 5.99e-105 320
Msa0997550 AT1G15150.1 36.688 477 259 5 4 439 9 483 1.53e-104 319
Msa0997550 AT1G66760.2 37.367 471 253 6 2 433 4 471 9.26e-103 314
Msa0997550 AT2G04070.1 40.090 444 226 5 26 431 27 468 1.47e-101 311
Msa0997550 AT2G04100.1 38.667 450 236 6 20 431 24 471 2.04e-100 308
Msa0997550 AT2G04080.1 38.964 444 231 5 26 431 27 468 2.33e-100 308
Msa0997550 AT2G04050.1 39.865 444 227 5 26 431 27 468 2.65e-100 308
Msa0997550 AT1G66760.1 37.310 461 247 6 2 423 4 461 1.14e-99 306
Msa0997550 AT2G04040.1 39.597 447 230 4 23 431 24 468 1.21e-99 306
Msa0997550 AT1G15180.1 39.703 471 242 5 7 437 14 482 2.32e-98 303
Msa0997550 AT2G04090.1 38.170 448 237 6 22 431 26 471 1.57e-97 301
Msa0997550 AT2G04100.2 38.462 442 232 6 20 423 24 463 3.91e-97 300
Msa0997550 AT2G04080.2 37.978 445 236 5 19 425 20 462 5.56e-97 299
Msa0997550 AT3G23550.1 38.673 437 228 4 24 422 29 463 1.50e-94 293
Msa0997550 AT1G15170.2 42.033 364 190 4 93 437 1 362 1.72e-93 286
Msa0997550 AT3G23560.1 37.554 466 241 5 5 422 8 471 2.54e-93 290
Msa0997550 AT1G61890.1 32.990 485 270 7 2 433 8 490 3.26e-89 280
Msa0997550 AT1G66780.1 37.324 426 227 4 44 431 53 476 4.13e-89 279
Msa0997550 AT1G15150.2 39.024 369 201 6 93 439 1 367 3.41e-87 271
Msa0997550 AT1G64820.1 36.613 437 237 5 33 431 36 470 3.77e-87 275
Msa0997550 AT1G61890.2 33.053 475 263 7 2 423 8 480 3.43e-86 272
Msa0997550 AT1G11670.1 32.599 454 266 5 26 441 50 501 1.32e-85 271
Msa0997550 AT3G59030.1 34.979 486 262 8 3 436 14 497 4.67e-85 270
Msa0997550 AT3G21690.1 34.292 452 257 5 20 433 46 495 1.09e-84 268
Msa0997550 AT4G21910.2 35.022 454 252 7 20 432 47 498 7.09e-82 261
Msa0997550 AT4G21910.4 35.022 454 252 7 20 432 47 498 7.09e-82 261
Msa0997550 AT4G21910.3 34.581 454 254 7 20 432 45 496 3.30e-81 259
Msa0997550 AT4G21910.1 34.581 454 254 7 20 432 45 496 3.30e-81 259
Msa0997550 AT1G66760.3 41.265 332 174 3 111 423 4 333 2.60e-78 247
Msa0997550 AT4G21903.1 34.651 430 242 6 40 432 67 494 5.34e-77 248
Msa0997550 AT4G21903.2 34.305 446 244 8 40 440 67 508 7.57e-75 243
Msa0997550 AT4G21903.3 35.676 370 217 4 82 432 18 385 8.55e-74 237
Msa0997550 AT1G23300.1 32.527 455 258 8 21 433 39 486 1.29e-73 240
Msa0997550 AT1G61890.3 32.063 446 248 7 2 394 8 451 4.82e-73 237
Msa0997550 AT1G66760.4 35.369 393 212 6 2 355 4 393 1.21e-71 231
Msa0997550 AT1G58340.1 33.559 444 255 4 24 429 56 497 2.68e-69 229
Msa0997550 AT1G12950.1 32.054 443 259 8 30 433 70 509 3.84e-69 228
Msa0997550 AT3G26590.1 30.599 451 272 7 21 433 40 487 1.17e-68 226
Msa0997550 AT1G47530.1 33.121 471 268 9 4 433 13 477 1.18e-68 226
Msa0997550 AT5G38030.1 30.942 446 267 7 26 433 45 487 2.08e-68 226
Msa0997550 AT3G23560.2 36.591 399 203 5 5 355 8 404 2.98e-68 223
Msa0997550 AT4G00350.1 32.088 455 259 9 22 433 82 529 5.95e-68 226
Msa0997550 AT4G29140.1 31.749 463 269 5 10 427 33 493 1.72e-67 224
Msa0997550 AT2G38510.1 30.837 454 272 9 25 439 5 455 2.13e-67 223
Msa0997550 AT5G65380.1 32.773 476 263 10 3 433 17 480 9.01e-65 216
Msa0997550 AT4G23030.1 33.482 448 251 7 24 429 30 472 1.36e-64 216
Msa0997550 AT3G26590.2 33.062 369 225 6 84 433 20 385 1.38e-63 210
Msa0997550 AT5G44050.1 32.623 469 261 12 13 433 22 483 1.17e-62 211
Msa0997550 AT1G47530.2 35.484 372 214 6 83 433 19 385 1.71e-62 207
Msa0997550 AT1G33090.1 31.027 477 280 11 8 439 12 484 1.56e-60 205
Msa0997550 AT1G71870.1 30.042 476 271 11 10 427 8 479 5.84e-60 204
Msa0997550 AT5G52050.1 31.963 438 254 7 33 427 42 478 6.34e-60 204
Msa0997550 AT1G33100.1 30.737 475 281 11 8 439 12 481 8.16e-60 203
Msa0997550 AT1G15180.2 39.223 283 151 3 93 356 1 281 3.38e-59 199
Msa0997550 AT3G59030.2 34.321 405 212 8 3 355 14 416 7.58e-59 199
Msa0997550 AT5G19700.1 30.316 475 283 6 9 437 16 488 9.42e-59 201
Msa0997550 AT1G33110.1 30.042 476 286 10 8 439 12 484 1.77e-58 199
Msa0997550 AT5G10420.1 31.343 469 276 9 14 439 22 487 1.28e-56 194
Msa0997550 AT1G33080.1 29.979 477 285 11 8 439 12 484 1.19e-55 192
Msa0997550 AT1G33080.2 30.538 465 274 11 8 427 12 472 5.41e-55 190
Msa0997550 AT5G49130.1 28.024 496 289 11 7 440 1 490 5.97e-55 191
Msa0997550 AT5G17700.2 29.828 466 278 13 11 433 17 476 9.11e-54 187
Msa0997550 AT5G17700.1 29.828 466 278 13 11 433 17 476 9.11e-54 187
Msa0997550 AT3G03620.2 29.614 466 278 13 11 432 19 478 1.56e-51 181
Msa0997550 AT3G03620.1 29.614 466 278 13 11 432 19 478 1.56e-51 181
Msa0997550 AT4G25640.1 30.103 485 277 12 1 433 1 475 2.84e-49 175
Msa0997550 AT4G25640.2 30.103 485 277 12 1 433 1 475 4.38e-49 175
Msa0997550 AT4G22790.1 28.764 445 279 7 21 427 25 469 1.04e-45 165
Msa0997550 AT4G25640.3 29.730 444 250 12 1 392 1 434 7.80e-42 154
Msa0997550 AT2G04066.1 47.692 130 65 1 310 439 42 168 3.60e-36 131
Msa0997550 AT1G33110.2 28.753 393 231 11 8 355 12 400 1.37e-34 133
Msa0997550 AT5G17700.3 28.021 389 231 13 10 355 16 398 2.02e-31 124

Find 96 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTAATGGTTCTCCTTTC+TGG 0.099766 6_4:-77507748 Msa0997550:CDS
TTATACTAGTTCGTAATTTA+TGG 0.163853 6_4:-77507177 Msa0997550:CDS
CTATTCCCCTCGCGATTATT+TGG 0.190931 6_4:-77508356 Msa0997550:CDS
TGTTACTGGTTTCAGTTTAT+TGG 0.197279 6_4:-77510296 Msa0997550:intron
GGAATGCAAGCTGTTCTTTC+AGG 0.202710 6_4:-77507070 Msa0997550:intron
ATGGCAAGCTTCAGGAAAAT+TGG 0.241777 6_4:+77507960 None:intergenic
CTTCCTCTATGTTGGATAAA+TGG 0.246267 6_4:-77508141 Msa0997550:CDS
CTGTAGATATTTCATGATCT+TGG 0.274075 6_4:+77508301 None:intergenic
GATGTTTCCAACTTTGGATT+TGG 0.287173 6_4:+77507718 None:intergenic
GAGAATGAAGAGACATGTTT+TGG 0.306641 6_4:+77510524 None:intergenic
TATTCCCCTCGCGATTATTT+GGG 0.313645 6_4:-77508355 Msa0997550:CDS
AATGTGGTCATTTGAATTAA+TGG 0.315453 6_4:-77507761 Msa0997550:CDS
CTTATGTTTGTTGGTCATCT+TGG 0.328499 6_4:-77510364 Msa0997550:CDS
TCAGTTACACTCTTAAATTA+TGG 0.341516 6_4:-77510403 Msa0997550:CDS
CTTGGATCATATTATTTAGT+TGG 0.342797 6_4:-77506678 Msa0997550:CDS
CTGAAGGAATGGCAAGCTTC+AGG 0.354381 6_4:+77507952 None:intergenic
ATTATTTCACTTATGTTTGT+TGG 0.354686 6_4:-77510373 Msa0997550:CDS
TCATCCACAGGCTGCGCGTT+TGG 0.355776 6_4:-77507257 Msa0997550:CDS
GGATGTGGTTGGCAGAAAAT+TGG 0.357552 6_4:-77506714 Msa0997550:CDS
TTTAACATAGAGTGCGAGTA+TGG 0.357733 6_4:+77508049 None:intergenic
TATACTAGTTCGTAATTTAT+GGG 0.361051 6_4:-77507176 Msa0997550:CDS
ATCTTTCAATGCACTGTTCA+AGG 0.363947 6_4:-77506215 Msa0997550:three_prime_UTR
TGGCAGCTATGCAAAGTTAA+TGG 0.367835 6_4:-77508274 Msa0997550:CDS
GTAGAAGACCATAAGCAAAA+AGG 0.373145 6_4:+77508244 None:intergenic
GGAAGAAGAAAGAAGAAGAT+TGG 0.389475 6_4:-77510470 Msa0997550:CDS
TTGGATCATATTATTTAGTT+GGG 0.395886 6_4:-77506677 Msa0997550:CDS
AAGACACAGTTATGGTTATC+AGG 0.397761 6_4:-77510436 Msa0997550:CDS
TGCCATTCCTTCAGCTGTAA+TGG 0.404862 6_4:-77507943 Msa0997550:CDS
CTTGGTGAATTGTCTCTCTC+TGG 0.419399 6_4:-77510346 Msa0997550:CDS
TAAATACAACTGCAGCTGTT+TGG 0.421440 6_4:-77507494 Msa0997550:CDS
ATGATCCCCTTCGGACTTAG+TGG 0.428230 6_4:-77507471 Msa0997550:CDS
TACTTCATCTTCCTCTATGT+TGG 0.435862 6_4:-77508149 Msa0997550:CDS
GTCTCTCTCTGGTGCTTCCA+TGG 0.436078 6_4:-77510335 Msa0997550:CDS
GTTCAGCGCAAGTGCTTCCT+TGG 0.439233 6_4:+77507989 None:intergenic
CTCCTTCATGTAAGAAAACA+TGG 0.439623 6_4:-77508022 Msa0997550:CDS
ATAATATAGGGCCTGTGGCT+GGG 0.439811 6_4:-77506533 Msa0997550:intron
TAGCAAATTCAATATCGTAC+TGG 0.441020 6_4:-77508082 Msa0997550:CDS
TAATATAGGGCCTGTGGCTG+GGG 0.444477 6_4:-77506532 Msa0997550:intron
GCAAAGCAGTACCGCATGTC+AGG 0.449733 6_4:-77508420 Msa0997550:intron
TGGCAAGCTTCAGGAAAATT+GGG 0.459148 6_4:+77507961 None:intergenic
TTCCTTCAGCTGTAATGGTC+TGG 0.465617 6_4:-77507938 Msa0997550:intron
AAGTGGGATACGGCAAGAAT+TGG 0.472050 6_4:+77507100 None:intergenic
TCATTACAATACGCACAGAT+TGG 0.479725 6_4:-77506469 Msa0997550:CDS
ATTCGTGTTTCGAATGAACT+TGG 0.485317 6_4:-77507286 Msa0997550:CDS
GCCGTATCCCACTTACTAGA+TGG 0.486822 6_4:-77507091 Msa0997550:CDS
AGAAGCAAAAGAAGTAGCCA+TGG 0.492944 6_4:+77510318 None:intergenic
GACTAAGATGCTCTCAATAA+CGG 0.495838 6_4:+77507210 None:intergenic
CATGAAATATCTACAGAAGC+TGG 0.501346 6_4:-77508294 Msa0997550:CDS
TTTGTTTCTTCAACAATCAA+AGG 0.503333 6_4:+77510502 None:intergenic
CTTGCATTCCATCTAGTAAG+TGG 0.503895 6_4:+77507083 None:intergenic
GCTGTTTGGATGATCCCCTT+CGG 0.508428 6_4:-77507480 Msa0997550:CDS
AAAGCGAATACAATGGCTGC+TGG 0.511302 6_4:+77506651 None:intergenic
TCGGACTTAGTGGAGCTGTA+AGG 0.514444 6_4:-77507461 Msa0997550:intron
CATTACAATACGCACAGATT+GGG 0.515621 6_4:-77506468 Msa0997550:CDS
ATTGGCGCATTTGTCAACCT+TGG 0.517716 6_4:-77506696 Msa0997550:CDS
TTGGCCAAGTAAAATGAGAA+TGG 0.519826 6_4:+77508320 None:intergenic
TGCACTGCATATTGGCGGGA+AGG 0.532183 6_4:-77506628 Msa0997550:intron
CACATATAATCCCCAGCCAC+AGG 0.532483 6_4:+77506522 None:intergenic
GACCATTACAGCTGAAGGAA+TGG 0.532522 6_4:+77507941 None:intergenic
TTTCTTAGAGTATTTGGAAG+GGG 0.536460 6_4:-77506242 Msa0997550:three_prime_UTR
AAGAAGTGAAGACACAGTTA+TGG 0.538327 6_4:-77510444 Msa0997550:CDS
GTACGATATTGAATTTGCTA+TGG 0.543345 6_4:+77508085 None:intergenic
CGATGAGCAGCTTGGAAATG+TGG 0.543480 6_4:-77507777 Msa0997550:intron
TCCATCTAGTAAGTGGGATA+CGG 0.545689 6_4:+77507090 None:intergenic
TCATGTAAGAAAACATGGAA+TGG 0.547867 6_4:-77508017 Msa0997550:CDS
AAGAGTGTAACTGATATCAA+TGG 0.547934 6_4:+77510412 None:intergenic
ACGCACAGATTGGGAGAAAG+AGG 0.549190 6_4:-77506459 Msa0997550:intron
GTTTCGAATGAACTTGGTGC+TGG 0.552325 6_4:-77507280 Msa0997550:CDS
AGCAAATTCAATATCGTACT+GGG 0.552376 6_4:-77508081 Msa0997550:CDS
TCAGGTACTGCTAGAGGATG+TGG 0.553165 6_4:-77506729 Msa0997550:intron
CAACTAAATAATATGATCCA+AGG 0.560535 6_4:+77506679 None:intergenic
ATACTAGTTCGTAATTTATG+GGG 0.564636 6_4:-77507175 Msa0997550:CDS
TACCAGACCATTACAGCTGA+AGG 0.567961 6_4:+77507936 None:intergenic
AAGTCTGGATTAGGTAGCAG+AGG 0.573228 6_4:-77508113 Msa0997550:intron
ATACCATTTATCCAACATAG+AGG 0.581555 6_4:+77508138 None:intergenic
ACTTGGTGCTGGTCATCCAC+AGG 0.587929 6_4:-77507269 Msa0997550:CDS
TACAGCTCCACTAAGTCCGA+AGG 0.592565 6_4:+77507464 None:intergenic
ATGAAGTAATGTTGTAACAG+TGG 0.599612 6_4:+77508164 None:intergenic
TGAGAGCATCTTAGTCGGAA+CGG 0.605010 6_4:-77507203 Msa0997550:CDS
TATTTATAATATAGGGCCTG+TGG 0.609405 6_4:-77506538 Msa0997550:intron
ACAGCTCCACTAAGTCCGAA+GGG 0.616463 6_4:+77507465 None:intergenic
TTGCATTCCATCTAGTAAGT+GGG 0.618025 6_4:+77507084 None:intergenic
GTTTGCCCAAATAATCGCGA+GGG 0.618210 6_4:+77508350 None:intergenic
TATGATTCAGGTACTGCTAG+AGG 0.620194 6_4:-77506735 Msa0997550:intron
GATTTGGAAGAAGACCAGAA+AGG 0.631676 6_4:+77507734 None:intergenic
GTTATTGAGAGCATCTTAGT+CGG 0.638872 6_4:-77507208 Msa0997550:CDS
TGTTTGCCCAAATAATCGCG+AGG 0.649848 6_4:+77508349 None:intergenic
TGAAGAAACAAAACAAAACA+AGG 0.651044 6_4:-77510491 Msa0997550:CDS
GACAGTCATGAGAATGAACA+TGG 0.656632 6_4:+77508380 None:intergenic
GACAAAGCGCTTGAACAACG+AGG 0.670556 6_4:+77506498 None:intergenic
GTACTGCTAGAGGATGTGGT+TGG 0.674177 6_4:-77506725 Msa0997550:CDS
CAGTGCAAAAGCGAATACAA+TGG 0.677120 6_4:+77506644 None:intergenic
CAGCTCCACTAAGTCCGAAG+GGG 0.684598 6_4:+77507466 None:intergenic
ACAAAGCGCTTGAACAACGA+GGG 0.691240 6_4:+77506499 None:intergenic
TTTGCCCAAATAATCGCGAG+GGG 0.704818 6_4:+77508351 None:intergenic
GCATGTGTATGCCTGACATG+CGG 0.723702 6_4:+77508409 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAAATATTTATAATATA+GGG - chr6_4:77510143-77510162 Msa0997550:intron 0.0%
!!! TAACTAATGTATTTTTTTTT+TGG - chr6_4:77506584-77506603 Msa0997550:intron 10.0%
!!! GTAAAACTTTTTTTTTTTTT+TGG + chr6_4:77507101-77507120 None:intergenic 10.0%
!! ATTATTCAAACTTAAATTTA+TGG - chr6_4:77508050-77508069 Msa0997550:CDS 10.0%
!! TAAAATTCTATTATAATATG+TGG + chr6_4:77508804-77508823 None:intergenic 10.0%
!! TATTATAAATATTTATTAGC+AGG + chr6_4:77510141-77510160 None:intergenic 10.0%
!!! AAAAAAAAATGATTTTTTTC+AGG + chr6_4:77510420-77510439 None:intergenic 10.0%
!!! AAATTTTTCAAGGAAAAAAA+TGG + chr6_4:77506437-77506456 None:intergenic 15.0%
!! ATAATGTAACAATGTTAATT+TGG - chr6_4:77506933-77506952 Msa0997550:intron 15.0%
!!! CTTTTTATATATGTAAATTC+CGG + chr6_4:77507145-77507164 None:intergenic 15.0%
!! TAAAGAATTTGTCAATTATA+AGG - chr6_4:77507669-77507688 Msa0997550:intron 15.0%
!!! TTGTTTTTGATATAATAGAT+AGG - chr6_4:77508202-77508221 Msa0997550:intron 15.0%
!! TCACATGAATATTTAATTAA+AGG + chr6_4:77510290-77510309 None:intergenic 15.0%
!! AAATGAATCAGAAAATATAA+AGG + chr6_4:77510335-77510354 None:intergenic 15.0%
!! ATTATTTCACTTATGTTTGT+TGG - chr6_4:77506315-77506334 Msa0997550:CDS 20.0%
!!! TCTGATTATGAAATTTTTCA+AGG + chr6_4:77506447-77506466 None:intergenic 20.0%
!! ATTATAAATGAGAACTTCTA+CGG - chr6_4:77506486-77506505 Msa0997550:CDS 20.0%
!!! TAAAGTTTGAGTTTCTTTTT+TGG - chr6_4:77506772-77506791 Msa0997550:intron 20.0%
!!! TTTAGTCTCTAAAAAATCAT+GGG - chr6_4:77506896-77506915 Msa0997550:intron 20.0%
!!! AAAAAAAAGTTTTACGCTTA+TGG - chr6_4:77507106-77507125 Msa0997550:CDS 20.0%
!! TTATGGTATAAATTCTTAAC+CGG - chr6_4:77507123-77507142 Msa0997550:CDS 20.0%
!! ATTCATTTCATTAACAGAAA+TGG + chr6_4:77507403-77507422 None:intergenic 20.0%
!!! GATAATTTTTCTTGATAACT+TGG - chr6_4:77507604-77507623 Msa0997550:intron 20.0%
!! CAAATTCTTTACAATGAATA+TGG + chr6_4:77507660-77507679 None:intergenic 20.0%
!! TTTGATATAATAGATAGGAA+TGG - chr6_4:77508207-77508226 Msa0997550:intron 20.0%
!! TAGGATTAAGTTAAATAGTA+AGG + chr6_4:77509270-77509289 None:intergenic 20.0%
!! TAAAAAAGAGAAAAAAGCTT+TGG + chr6_4:77509299-77509318 None:intergenic 20.0%
!! TTATACTAGTTCGTAATTTA+TGG - chr6_4:77509511-77509530 Msa0997550:intron 20.0%
!! TATACTAGTTCGTAATTTAT+GGG - chr6_4:77509512-77509531 Msa0997550:intron 20.0%
!! ACAACAAAATAAAAAGTTGA+TGG + chr6_4:77509653-77509672 None:intergenic 20.0%
!! CTAATATATCTGATACAAAA+AGG - chr6_4:77509719-77509738 Msa0997550:intron 20.0%
!!! TACAAAAAGGTAACTAATTT+TGG - chr6_4:77509732-77509751 Msa0997550:intron 20.0%
!! GATCGAAAAAAATAAATAAC+AGG - chr6_4:77509832-77509851 Msa0997550:intron 20.0%
!! TTGGATCATATTATTTAGTT+GGG - chr6_4:77510011-77510030 Msa0997550:intron 20.0%
!!! TTTTGTTTTCTTAGAGTATT+TGG - chr6_4:77510440-77510459 Msa0997550:CDS 20.0%
!! TTTGTTTCTTCAACAATCAA+AGG + chr6_4:77506189-77506208 None:intergenic 25.0%
! TGAAGAAACAAAACAAAACA+AGG - chr6_4:77506197-77506216 Msa0997550:three_prime_UTR 25.0%
! TCAGTTACACTCTTAAATTA+TGG - chr6_4:77506285-77506304 Msa0997550:exon 25.0%
! TACCACTAAGATTACAAAAT+TGG + chr6_4:77506870-77506889 None:intergenic 25.0%
!! CTTTAGTCTCTAAAAAATCA+TGG - chr6_4:77506895-77506914 Msa0997550:intron 25.0%
!!! GGATGAAAATTTTTGTTTAC+TGG - chr6_4:77506954-77506973 Msa0997550:intron 25.0%
!!! GTTCTAGTTTAACTTAAACA+TGG + chr6_4:77507059-77507078 None:intergenic 25.0%
! CAATAATTCATAAAACCCTA+GGG + chr6_4:77507301-77507320 None:intergenic 25.0%
!!! CTAGGGTTTTATGAATTATT+GGG - chr6_4:77507300-77507319 Msa0997550:intron 25.0%
! CTTGTGTTGAATACATATTT+GGG + chr6_4:77507365-77507384 None:intergenic 25.0%
! CAAATATGTATTCAACACAA+GGG - chr6_4:77507364-77507383 Msa0997550:intron 25.0%
! TCACAATGAATAAGAACATA+TGG + chr6_4:77507535-77507554 None:intergenic 25.0%
! TTCTTATTCATTGTGATGTT+TGG - chr6_4:77507539-77507558 Msa0997550:intron 25.0%
! TCTTATTCATTGTGATGTTT+GGG - chr6_4:77507540-77507559 Msa0997550:intron 25.0%
! ATGATTAATTAGATAAGCAG+AGG + chr6_4:77507841-77507860 None:intergenic 25.0%
!! TACTAACCTTATCTGAAATA+CGG + chr6_4:77507872-77507891 None:intergenic 25.0%
!! AGACTAAACTTGTTCATAAT+TGG - chr6_4:77508021-77508040 Msa0997550:CDS 25.0%
!! TTTTCCTATACTCTAATAGT+AGG - chr6_4:77508113-77508132 Msa0997550:intron 25.0%
!!! ATTTTTGGACAAAATGACAA+AGG + chr6_4:77508168-77508187 None:intergenic 25.0%
! AATGTGGTCATTTGAATTAA+TGG - chr6_4:77508927-77508946 Msa0997550:intron 25.0%
!!! AAAAAAGCTTTGGAGAATTT+AGG + chr6_4:77509289-77509308 None:intergenic 25.0%
! ATACTAGTTCGTAATTTATG+GGG - chr6_4:77509513-77509532 Msa0997550:intron 25.0%
!! TTTGTTGTCTTCATAAGATT+TGG - chr6_4:77509665-77509684 Msa0997550:intron 25.0%
! TTGTTGTCTTCATAAGATTT+GGG - chr6_4:77509666-77509685 Msa0997550:intron 25.0%
! CAACTAAATAATATGATCCA+AGG + chr6_4:77510012-77510031 None:intergenic 25.0%
! CTTGGATCATATTATTTAGT+TGG - chr6_4:77510010-77510029 Msa0997550:intron 25.0%
! AAAATCGCTATCATAAGATT+TGG - chr6_4:77510110-77510129 Msa0997550:intron 25.0%
!! TTTTCTGATTCATTTTGTAC+AGG - chr6_4:77510340-77510359 Msa0997550:CDS 25.0%
!!! TTTTCTTAGAGTATTTGGAA+GGG - chr6_4:77510445-77510464 Msa0997550:CDS 25.0%
! AAGAGTGTAACTGATATCAA+TGG + chr6_4:77506279-77506298 None:intergenic 30.0%
!! TGTTACTGGTTTCAGTTTAT+TGG - chr6_4:77506392-77506411 Msa0997550:intron 30.0%
TACGGTACACTCATAAATTA+TGG - chr6_4:77506504-77506523 Msa0997550:CDS 30.0%
ACTCATAAATTATGGTGCAT+TGG - chr6_4:77506512-77506531 Msa0997550:CDS 30.0%
!!! GGTTGTCAGAAATTTTTTGA+AGG - chr6_4:77506793-77506812 Msa0997550:intron 30.0%
TGCAGGAAAACATTAATACA+AGG - chr6_4:77506840-77506859 Msa0997550:intron 30.0%
! GTCCAATTTTGTAATCTTAG+TGG - chr6_4:77506865-77506884 Msa0997550:intron 30.0%
AAAAATCATGGGTTCAATTG+TGG - chr6_4:77506907-77506926 Msa0997550:intron 30.0%
ATCTCACATTTAGAAACAAG+AGG - chr6_4:77507026-77507045 Msa0997550:intron 30.0%
CCAATAATTCATAAAACCCT+AGG + chr6_4:77507302-77507321 None:intergenic 30.0%
!! CCTAGGGTTTTATGAATTAT+TGG - chr6_4:77507299-77507318 Msa0997550:intron 30.0%
CCTTGTGTTGAATACATATT+TGG + chr6_4:77507366-77507385 None:intergenic 30.0%
CCAAATATGTATTCAACACA+AGG - chr6_4:77507363-77507382 Msa0997550:intron 30.0%
CATTTCATTAACAGAAATGG+AGG + chr6_4:77507400-77507419 None:intergenic 30.0%
CTTATTCATTGTGATGTTTG+GGG - chr6_4:77507541-77507560 Msa0997550:intron 30.0%
TTATTCATTGTGATGTTTGG+GGG - chr6_4:77507542-77507561 Msa0997550:intron 30.0%
!! TTGCAACACCATTTTGATAT+GGG + chr6_4:77507729-77507748 None:intergenic 30.0%
!! TTTGCAACACCATTTTGATA+TGG + chr6_4:77507730-77507749 None:intergenic 30.0%
GAGCAACTTAAAGTAAATGA+CGG + chr6_4:77507762-77507781 None:intergenic 30.0%
!! TCATTTACTTTAAGTTGCTC+AGG - chr6_4:77507762-77507781 Msa0997550:CDS 30.0%
! ACTAACCTTATCTGAAATAC+GGG + chr6_4:77507871-77507890 None:intergenic 30.0%
!!! TTCCTATTTTTAATCTCTCG+TGG - chr6_4:77507921-77507940 Msa0997550:intron 30.0%
AACCACGAGAGATTAAAAAT+AGG + chr6_4:77507926-77507945 None:intergenic 30.0%
AGAGAAAATAGAGGGAAATA+TGG + chr6_4:77507958-77507977 None:intergenic 30.0%
TAGAGTTGAGAGAAAATAGA+GGG + chr6_4:77507966-77507985 None:intergenic 30.0%
TTAGAGTTGAGAGAAAATAG+AGG + chr6_4:77507967-77507986 None:intergenic 30.0%
! AGTTTAGTCTTCAAGGTAAT+GGG + chr6_4:77508011-77508030 None:intergenic 30.0%
! AAGTTTAGTCTTCAAGGTAA+TGG + chr6_4:77508012-77508031 None:intergenic 30.0%
! ATGAACAAGTTTAGTCTTCA+AGG + chr6_4:77508018-77508037 None:intergenic 30.0%
AACACCTACTATTAGAGTAT+AGG + chr6_4:77508120-77508139 None:intergenic 30.0%
ACATTATGTGGACAATCATA+TGG - chr6_4:77508244-77508263 Msa0997550:CDS 30.0%
! AGATCCATTCTCATTTTACT+TGG - chr6_4:77508364-77508383 Msa0997550:CDS 30.0%
CTGTAGATATTTCATGATCT+TGG + chr6_4:77508390-77508409 None:intergenic 30.0%
ATGAAGTAATGTTGTAACAG+TGG + chr6_4:77508527-77508546 None:intergenic 30.0%
ATACCATTTATCCAACATAG+AGG + chr6_4:77508553-77508572 None:intergenic 30.0%
!! GTACGATATTGAATTTGCTA+TGG + chr6_4:77508606-77508625 None:intergenic 30.0%
TAGCAAATTCAATATCGTAC+TGG - chr6_4:77508606-77508625 Msa0997550:intron 30.0%
AGCAAATTCAATATCGTACT+GGG - chr6_4:77508607-77508626 Msa0997550:intron 30.0%
! TTCCATGTTTTCTTACATGA+AGG + chr6_4:77508671-77508690 None:intergenic 30.0%
TCATGTAAGAAAACATGGAA+TGG - chr6_4:77508671-77508690 Msa0997550:intron 30.0%
!! TTAACCTTTTGTTTGTGACT+TGG - chr6_4:77509160-77509179 Msa0997550:intron 30.0%
! TAGCGATTTTCATGAAAGAA+TGG + chr6_4:77510100-77510119 None:intergenic 30.0%
TATTTATAATATAGGGCCTG+TGG - chr6_4:77510150-77510169 Msa0997550:intron 30.0%
AATATTCATGTGATGCATTG+TGG - chr6_4:77510297-77510316 Msa0997550:CDS 30.0%
ATATTCATGTGATGCATTGT+GGG - chr6_4:77510298-77510317 Msa0997550:CDS 30.0%
!! GTTTTCTTAGAGTATTTGGA+AGG - chr6_4:77510444-77510463 Msa0997550:CDS 30.0%
! TTTCTTAGAGTATTTGGAAG+GGG - chr6_4:77510446-77510465 Msa0997550:CDS 30.0%
! GAGAATGAAGAGACATGTTT+TGG + chr6_4:77506167-77506186 None:intergenic 35.0%
GGAAGAAGAAAGAAGAAGAT+TGG - chr6_4:77506218-77506237 Msa0997550:three_prime_UTR 35.0%
AAGAAGTGAAGACACAGTTA+TGG - chr6_4:77506244-77506263 Msa0997550:three_prime_UTR 35.0%
AAGACACAGTTATGGTTATC+AGG - chr6_4:77506252-77506271 Msa0997550:three_prime_UTR 35.0%
! CTTATGTTTGTTGGTCATCT+TGG - chr6_4:77506324-77506343 Msa0997550:CDS 35.0%
! ACTTCTTTTGCTTCTGTTAC+TGG - chr6_4:77506378-77506397 Msa0997550:intron 35.0%
ATGTGAGTTTGTGTGAACTA+AGG - chr6_4:77506630-77506649 Msa0997550:CDS 35.0%
ACAATCCTAGAATTTGTACC+TGG - chr6_4:77507217-77507236 Msa0997550:CDS 35.0%
CTTAACCAGGTACAAATTCT+AGG + chr6_4:77507225-77507244 None:intergenic 35.0%
CTAGAATTTGTACCTGGTTA+AGG - chr6_4:77507223-77507242 Msa0997550:CDS 35.0%
AATTTGTACCTGGTTAAGGA+TGG - chr6_4:77507227-77507246 Msa0997550:CDS 35.0%
! TGATTTGGACATGACAATGA+AGG + chr6_4:77507327-77507346 None:intergenic 35.0%
TCATTGTCATGTCCAAATCA+CGG - chr6_4:77507327-77507346 Msa0997550:intron 35.0%
TCATTAACAGAAATGGAGGA+TGG + chr6_4:77507396-77507415 None:intergenic 35.0%
CAAACCATTCCTTGAAACAA+GGG + chr6_4:77507573-77507592 None:intergenic 35.0%
ACAAACCATTCCTTGAAACA+AGG + chr6_4:77507574-77507593 None:intergenic 35.0%
!! TGCAACACCATTTTGATATG+GGG + chr6_4:77507728-77507747 None:intergenic 35.0%
!! TCTTCAAGGTAATGGGAAAA+GGG + chr6_4:77508004-77508023 None:intergenic 35.0%
TTGTCCACATAATGTGTCTA+AGG + chr6_4:77508239-77508258 None:intergenic 35.0%
TTGGCCAAGTAAAATGAGAA+TGG + chr6_4:77508371-77508390 None:intergenic 35.0%
CATGAAATATCTACAGAAGC+TGG - chr6_4:77508394-77508413 Msa0997550:CDS 35.0%
GTAGAAGACCATAAGCAAAA+AGG + chr6_4:77508447-77508466 None:intergenic 35.0%
TACTTCATCTTCCTCTATGT+TGG - chr6_4:77508539-77508558 Msa0997550:intron 35.0%
CTTCCTCTATGTTGGATAAA+TGG - chr6_4:77508547-77508566 Msa0997550:intron 35.0%
GGATAAATGGTATACAAGTC+TGG - chr6_4:77508560-77508579 Msa0997550:intron 35.0%
ATGGTATACAAGTCTGGATT+AGG - chr6_4:77508566-77508585 Msa0997550:intron 35.0%
TTTAACATAGAGTGCGAGTA+TGG + chr6_4:77508642-77508661 None:intergenic 35.0%
CTCCTTCATGTAAGAAAACA+TGG - chr6_4:77508666-77508685 Msa0997550:intron 35.0%
! AACATGGAATGGTTTTTCCA+AGG - chr6_4:77508682-77508701 Msa0997550:intron 35.0%
! GTGTTATGAAGTACTAGAGT+AGG + chr6_4:77508782-77508801 None:intergenic 35.0%
GAATTAATGGTTCTCCTTTC+TGG - chr6_4:77508940-77508959 Msa0997550:intron 35.0%
TCTTCTTCCAAATCCAAAGT+TGG - chr6_4:77508963-77508982 Msa0997550:intron 35.0%
GATGTTTCCAACTTTGGATT+TGG + chr6_4:77508973-77508992 None:intergenic 35.0%
AAACATCTGTGCTTTCTATC+TGG - chr6_4:77508986-77509005 Msa0997550:intron 35.0%
AGAACCAAGTCACAAACAAA+AGG + chr6_4:77509167-77509186 None:intergenic 35.0%
TAAATACAACTGCAGCTGTT+TGG - chr6_4:77509194-77509213 Msa0997550:intron 35.0%
ATTCGTGTTTCGAATGAACT+TGG - chr6_4:77509402-77509421 Msa0997550:intron 35.0%
GACTAAGATGCTCTCAATAA+CGG + chr6_4:77509481-77509500 None:intergenic 35.0%
!! GTTATTGAGAGCATCTTAGT+CGG - chr6_4:77509480-77509499 Msa0997550:intron 35.0%
TTGCATTCCATCTAGTAAGT+GGG + chr6_4:77509607-77509626 None:intergenic 35.0%
TAAAAACAGACCGAGACATA+TGG - chr6_4:77509690-77509709 Msa0997550:intron 35.0%
AAAAACAGACCGAGACATAT+GGG - chr6_4:77509691-77509710 Msa0997550:intron 35.0%
TTAGAAGTTCCCATATGTCT+CGG + chr6_4:77509703-77509722 None:intergenic 35.0%
AAATAACAGGTCATCGAAGT+TGG - chr6_4:77509845-77509864 Msa0997550:intron 35.0%
TTGGCATCTCTAACACTTAT+TGG - chr6_4:77509864-77509883 Msa0997550:intron 35.0%
TCATTACAATACGCACAGAT+TGG - chr6_4:77510219-77510238 Msa0997550:intron 35.0%
CATTACAATACGCACAGATT+GGG - chr6_4:77510220-77510239 Msa0997550:intron 35.0%
TATTCATGTGATGCATTGTG+GGG - chr6_4:77510299-77510318 Msa0997550:CDS 35.0%
! TCATTTTGTACAGGCAAAGA+AGG - chr6_4:77510349-77510368 Msa0997550:CDS 35.0%
ATCTTTCAATGCACTGTTCA+AGG - chr6_4:77510473-77510492 Msa0997550:CDS 35.0%
AGAAGCAAAAGAAGTAGCCA+TGG + chr6_4:77506373-77506392 None:intergenic 40.0%
TGCATTCATGCAATCAGACA+TGG - chr6_4:77506682-77506701 Msa0997550:CDS 40.0%
!! GATGGATGTCTGGGATTTTA+GGG - chr6_4:77507245-77507264 Msa0997550:CDS 40.0%
TGTATTCAACACAAGGGTCT+TGG - chr6_4:77507370-77507389 Msa0997550:intron 40.0%
TGTCGACACATGTGAATTCA+TGG + chr6_4:77507705-77507724 None:intergenic 40.0%
GTCATCCCGTATTTCAGATA+AGG - chr6_4:77507863-77507882 Msa0997550:intron 40.0%
! GTAATGGGAAAAGGGAACAA+AGG + chr6_4:77507996-77508015 None:intergenic 40.0%
!! GTCTTCAAGGTAATGGGAAA+AGG + chr6_4:77508005-77508024 None:intergenic 40.0%
TGACAAAGGTTGAATCTCAG+TGG + chr6_4:77508154-77508173 None:intergenic 40.0%
AGTGCCTTAGACACATTATG+TGG - chr6_4:77508232-77508251 Msa0997550:CDS 40.0%
GACAGTCATGAGAATGAACA+TGG + chr6_4:77508311-77508330 None:intergenic 40.0%
TATTCCCCTCGCGATTATTT+GGG - chr6_4:77508333-77508352 Msa0997550:CDS 40.0%
TGGCAGCTATGCAAAGTTAA+TGG - chr6_4:77508414-77508433 Msa0997550:CDS 40.0%
! GTTCCATGCCTTTTTGCTTA+TGG - chr6_4:77508436-77508455 Msa0997550:intron 40.0%
AGACCATAAGCAAAAAGGCA+TGG + chr6_4:77508442-77508461 None:intergenic 40.0%
ACAGTGGAGCTAAACATCAT+TGG + chr6_4:77508511-77508530 None:intergenic 40.0%
TGGCAAGCTTCAGGAAAATT+GGG + chr6_4:77508730-77508749 None:intergenic 40.0%
ATGGCAAGCTTCAGGAAAAT+TGG + chr6_4:77508731-77508750 None:intergenic 40.0%
CATCGTGACGAGGAAAAAAA+TGG + chr6_4:77508896-77508915 None:intergenic 40.0%
GATTTGGAAGAAGACCAGAA+AGG + chr6_4:77508957-77508976 None:intergenic 40.0%
AGCACAGATGTTTCCAACTT+TGG + chr6_4:77508979-77508998 None:intergenic 40.0%
ATCTGTGCTTTCTATCTGGT+TGG - chr6_4:77508990-77509009 Msa0997550:intron 40.0%
GAGCTGTAAGGTTTGAATAC+AGG - chr6_4:77509239-77509258 Msa0997550:intron 40.0%
TCCATCTAGTAAGTGGGATA+CGG + chr6_4:77509601-77509620 None:intergenic 40.0%
CTTGCATTCCATCTAGTAAG+TGG + chr6_4:77509608-77509627 None:intergenic 40.0%
GATCTCCTTCACTATGATTC+AGG - chr6_4:77509941-77509960 Msa0997550:intron 40.0%
CAGTACCTGAATCATAGTGA+AGG + chr6_4:77509949-77509968 None:intergenic 40.0%
! TATGATTCAGGTACTGCTAG+AGG - chr6_4:77509953-77509972 Msa0997550:intron 40.0%
CAGTGCAAAAGCGAATACAA+TGG + chr6_4:77510047-77510066 None:intergenic 40.0%
!! TTCGCTTTTGCACTGCATAT+TGG - chr6_4:77510052-77510071 Msa0997550:intron 40.0%
GGCGGGAAGGTAAAAATAAA+TGG - chr6_4:77510073-77510092 Msa0997550:intron 40.0%
CTTGGTGAATTGTCTCTCTC+TGG - chr6_4:77506342-77506361 Msa0997550:intron 45.0%
TTCCTGCAATCGTGTAGTCA+GGG + chr6_4:77506828-77506847 None:intergenic 45.0%
TTTCCTGCAATCGTGTAGTC+AGG + chr6_4:77506829-77506848 None:intergenic 45.0%
CTGGTTAAGGATGGATGTCT+GGG - chr6_4:77507236-77507255 Msa0997550:CDS 45.0%
!! GGATGGATGTCTGGGATTTT+AGG - chr6_4:77507244-77507263 Msa0997550:CDS 45.0%
!!! GATTTTAGGGTGAGTGTGTG+TGG - chr6_4:77507258-77507277 Msa0997550:CDS 45.0%
ATTCATAAAACCCTAGGGCC+AGG + chr6_4:77507296-77507315 None:intergenic 45.0%
TTGTCATGTCCAAATCACGG+TGG - chr6_4:77507330-77507349 Msa0997550:intron 45.0%
TGTCATGTCCAAATCACGGT+GGG - chr6_4:77507331-77507350 Msa0997550:intron 45.0%
CATTGTGATGTTTGGGGGAT+TGG - chr6_4:77507547-77507566 Msa0997550:intron 45.0%
! TTGGCCCTTGTTTCAAGGAA+TGG - chr6_4:77507566-77507585 Msa0997550:intron 45.0%
GTCGACACCCCATATCAAAA+TGG - chr6_4:77507718-77507737 Msa0997550:intron 45.0%
GGTTGAATCTCAGTGGATCA+TGG + chr6_4:77508147-77508166 None:intergenic 45.0%
CTATTCCCCTCGCGATTATT+TGG - chr6_4:77508332-77508351 Msa0997550:CDS 45.0%
TTTGCCCAAATAATCGCGAG+GGG + chr6_4:77508340-77508359 None:intergenic 45.0%
GTTTGCCCAAATAATCGCGA+GGG + chr6_4:77508341-77508360 None:intergenic 45.0%
TGTTTGCCCAAATAATCGCG+AGG + chr6_4:77508342-77508361 None:intergenic 45.0%
! AAGTCTGGATTAGGTAGCAG+AGG - chr6_4:77508575-77508594 Msa0997550:intron 45.0%
TGCCATTCCTTCAGCTGTAA+TGG - chr6_4:77508745-77508764 Msa0997550:intron 45.0%
GACCATTACAGCTGAAGGAA+TGG + chr6_4:77508750-77508769 None:intergenic 45.0%
TTCCTTCAGCTGTAATGGTC+TGG - chr6_4:77508750-77508769 Msa0997550:intron 45.0%
TACCAGACCATTACAGCTGA+AGG + chr6_4:77508755-77508774 None:intergenic 45.0%
!! GTTTCGAATGAACTTGGTGC+TGG - chr6_4:77509408-77509427 Msa0997550:intron 45.0%
!! TGAGAGCATCTTAGTCGGAA+CGG - chr6_4:77509485-77509504 Msa0997550:intron 45.0%
AAGTGGGATACGGCAAGAAT+TGG + chr6_4:77509591-77509610 None:intergenic 45.0%
GGAATGCAAGCTGTTCTTTC+AGG - chr6_4:77509618-77509637 Msa0997550:intron 45.0%
GGATGTGGTTGGCAGAAAAT+TGG - chr6_4:77509974-77509993 Msa0997550:intron 45.0%
ATTGGCGCATTTGTCAACCT+TGG - chr6_4:77509992-77510011 Msa0997550:intron 45.0%
AAAGCGAATACAATGGCTGC+TGG + chr6_4:77510040-77510059 None:intergenic 45.0%
! GCTTTTGCACTGCATATTGG+CGG - chr6_4:77510055-77510074 Msa0997550:intron 45.0%
! CTTTTGCACTGCATATTGGC+GGG - chr6_4:77510056-77510075 Msa0997550:intron 45.0%
TATAATATAGGGCCTGTGGC+TGG - chr6_4:77510154-77510173 Msa0997550:intron 45.0%
ATAATATAGGGCCTGTGGCT+GGG - chr6_4:77510155-77510174 Msa0997550:intron 45.0%
! ACAAAGCGCTTGAACAACGA+GGG + chr6_4:77510192-77510211 None:intergenic 45.0%
!!! AATAACATTAAAATTATTTT+TGG + chr6_4:77508183-77508202 None:intergenic 5.0%
!! CTAATAAATATTTATAATAT+AGG - chr6_4:77510142-77510161 Msa0997550:intron 5.0%
CAATCGTGTAGTCAGGGTAC+TGG + chr6_4:77506822-77506841 None:intergenic 50.0%
TACCCTGACTACACGATTGC+AGG - chr6_4:77506823-77506842 Msa0997550:intron 50.0%
CCAGACATCCATCCTTAACC+AGG + chr6_4:77507238-77507257 None:intergenic 50.0%
CCTGGTTAAGGATGGATGTC+TGG - chr6_4:77507235-77507254 Msa0997550:CDS 50.0%
GTGTGGTCGAAAATCGATCC+TGG - chr6_4:77507275-77507294 Msa0997550:CDS 50.0%
GAAAATCGATCCTGGCCCTA+GGG - chr6_4:77507283-77507302 Msa0997550:CDS 50.0%
ACATGTAGCCCACCGTGATT+TGG + chr6_4:77507342-77507361 None:intergenic 50.0%
GCATGTGTATGCCTGACATG+CGG + chr6_4:77508282-77508301 None:intergenic 50.0%
CTGAAGGAATGGCAAGCTTC+AGG + chr6_4:77508739-77508758 None:intergenic 50.0%
CGATGAGCAGCTTGGAAATG+TGG - chr6_4:77508911-77508930 Msa0997550:intron 50.0%
GCTGTTTGGATGATCCCCTT+CGG - chr6_4:77509208-77509227 Msa0997550:intron 50.0%
ATGATCCCCTTCGGACTTAG+TGG - chr6_4:77509217-77509236 Msa0997550:intron 50.0%
ACAGCTCCACTAAGTCCGAA+GGG + chr6_4:77509226-77509245 None:intergenic 50.0%
TACAGCTCCACTAAGTCCGA+AGG + chr6_4:77509227-77509246 None:intergenic 50.0%
TCGGACTTAGTGGAGCTGTA+AGG - chr6_4:77509227-77509246 Msa0997550:intron 50.0%
GCCGTATCCCACTTACTAGA+TGG - chr6_4:77509597-77509616 Msa0997550:intron 50.0%
! TCAGGTACTGCTAGAGGATG+TGG - chr6_4:77509959-77509978 Msa0997550:intron 50.0%
GTACTGCTAGAGGATGTGGT+TGG - chr6_4:77509963-77509982 Msa0997550:intron 50.0%
TAATATAGGGCCTGTGGCTG+GGG - chr6_4:77510156-77510175 Msa0997550:intron 50.0%
CACATATAATCCCCAGCCAC+AGG + chr6_4:77510169-77510188 None:intergenic 50.0%
! GACAAAGCGCTTGAACAACG+AGG + chr6_4:77510193-77510212 None:intergenic 50.0%
ACGCACAGATTGGGAGAAAG+AGG - chr6_4:77510229-77510248 Msa0997550:intron 50.0%
GTCTCTCTCTGGTGCTTCCA+TGG - chr6_4:77506353-77506372 Msa0997550:intron 55.0%
!! TCGTGTAGTCAGGGTACTGG+TGG + chr6_4:77506819-77506838 None:intergenic 55.0%
CGAAAATCGATCCTGGCCCT+AGG - chr6_4:77507282-77507301 Msa0997550:CDS 55.0%
!! GGGGATTGGCCCTTGTTTCA+AGG - chr6_4:77507561-77507580 Msa0997550:intron 55.0%
GCAAAGCAGTACCGCATGTC+AGG - chr6_4:77508268-77508287 Msa0997550:CDS 55.0%
GTTCAGCGCAAGTGCTTCCT+TGG + chr6_4:77508702-77508721 None:intergenic 55.0%
CAGCTCCACTAAGTCCGAAG+GGG + chr6_4:77509225-77509244 None:intergenic 55.0%
!! ACTTGGTGCTGGTCATCCAC+AGG - chr6_4:77509419-77509438 Msa0997550:intron 55.0%
TGCACTGCATATTGGCGGGA+AGG - chr6_4:77510060-77510079 Msa0997550:intron 55.0%
CCAAGCTGCTCATCGTGACG+AGG + chr6_4:77508906-77508925 None:intergenic 60.0%
CCTCGTCACGATGAGCAGCT+TGG - chr6_4:77508903-77508922 Msa0997550:intron 60.0%
TCATCCACAGGCTGCGCGTT+TGG - chr6_4:77509431-77509450 Msa0997550:intron 60.0%
ACTGCCAAACGCGCAGCCTG+TGG + chr6_4:77509438-77509457 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 77506153 77510557 77506153 ID=Msa0997550;Name=Msa0997550
chr6_4 mRNA 77506153 77510557 77506153 ID=Msa0997550-mRNA-1;Parent=Msa0997550;Name=Msa0997550-mRNA-1;_AED=0.18;_eAED=0.18;_QI=18|0.55|0.6|1|0.44|0.5|10|136|442
chr6_4 exon 77506153 77506348 77506153 ID=Msa0997550-mRNA-1:exon:14587;Parent=Msa0997550-mRNA-1
chr6_4 exon 77506460 77506546 77506460 ID=Msa0997550-mRNA-1:exon:14586;Parent=Msa0997550-mRNA-1
chr6_4 exon 77506629 77506747 77506629 ID=Msa0997550-mRNA-1:exon:14585;Parent=Msa0997550-mRNA-1
chr6_4 exon 77507071 77507309 77507071 ID=Msa0997550-mRNA-1:exon:14584;Parent=Msa0997550-mRNA-1
chr6_4 exon 77507462 77507518 77507462 ID=Msa0997550-mRNA-1:exon:14583;Parent=Msa0997550-mRNA-1
chr6_4 exon 77507730 77507789 77507730 ID=Msa0997550-mRNA-1:exon:14582;Parent=Msa0997550-mRNA-1
chr6_4 exon 77507939 77508122 77507939 ID=Msa0997550-mRNA-1:exon:14581;Parent=Msa0997550-mRNA-1
chr6_4 exon 77508134 77508188 77508134 ID=Msa0997550-mRNA-1:exon:14580;Parent=Msa0997550-mRNA-1
chr6_4 exon 77508209 77508433 77508209 ID=Msa0997550-mRNA-1:exon:14579;Parent=Msa0997550-mRNA-1
chr6_4 exon 77510297 77510557 77510297 ID=Msa0997550-mRNA-1:exon:14578;Parent=Msa0997550-mRNA-1
chr6_4 five_prime_UTR 77510540 77510557 77510540 ID=Msa0997550-mRNA-1:five_prime_utr;Parent=Msa0997550-mRNA-1
chr6_4 CDS 77510297 77510539 77510297 ID=Msa0997550-mRNA-1:cds;Parent=Msa0997550-mRNA-1
chr6_4 CDS 77508209 77508433 77508209 ID=Msa0997550-mRNA-1:cds;Parent=Msa0997550-mRNA-1
chr6_4 CDS 77508134 77508188 77508134 ID=Msa0997550-mRNA-1:cds;Parent=Msa0997550-mRNA-1
chr6_4 CDS 77507939 77508122 77507939 ID=Msa0997550-mRNA-1:cds;Parent=Msa0997550-mRNA-1
chr6_4 CDS 77507730 77507789 77507730 ID=Msa0997550-mRNA-1:cds;Parent=Msa0997550-mRNA-1
chr6_4 CDS 77507462 77507518 77507462 ID=Msa0997550-mRNA-1:cds;Parent=Msa0997550-mRNA-1
chr6_4 CDS 77507071 77507309 77507071 ID=Msa0997550-mRNA-1:cds;Parent=Msa0997550-mRNA-1
chr6_4 CDS 77506629 77506747 77506629 ID=Msa0997550-mRNA-1:cds;Parent=Msa0997550-mRNA-1
chr6_4 CDS 77506460 77506546 77506460 ID=Msa0997550-mRNA-1:cds;Parent=Msa0997550-mRNA-1
chr6_4 CDS 77506289 77506348 77506289 ID=Msa0997550-mRNA-1:cds;Parent=Msa0997550-mRNA-1
chr6_4 three_prime_UTR 77506153 77506288 77506153 ID=Msa0997550-mRNA-1:three_prime_utr;Parent=Msa0997550-mRNA-1
Gene Sequence

>Msa0997550

ATGTCTCTTCATTCTCCTTTGATTGTTGAAGAAACAAAACAAAACAAGGAAGAAGAAAGAAGAAGATTGGTTGAAGAAGTGAAGACACAGTTATGGTTATCAGGACCATTGATATCAGTTACACTCTTAAATTATGGTATCAATATTATTTCACTTATGTTTGTTGGTCATCTTGGTGAATTGTCTCTCTCTGGTGCTTCCATGGCTACTTCTTTTGCTTCTGTTACTGGTTTCAGTTTATTGTACCGCATGTCAGGCATACACATGCAAAGAGCCATGTTCATTCTCATGACTGTCGCTATTCCCCTCGCGATTATTTGGGCAAACACAAGATCCATTCTCATTTTACTTGGCCAAGATCATGAAATATCTACAGAAGCTGGCAGCTATGCAAAGTTAATGGTTCCATGCCTTTTTGCTTATGGTCTTCTACAATGTCTTAACAGATTCTTACAGACTCAAAATATTCTCCACTGTTACAACATTACTTCATCTTCCTCTATGTTGGATAAATGGTATACAAGTAGCAGAGGAGCTGCCATAGCAAATTCAATATCGTACTGGGTGAATGTTACCATACTCGCACTCTATGTTAAATTTTCTCCTTCATGTAAGAAAACATGGAATGGTTTTTCCAAGGAAGCACTTGCGCTGAACAACATCCCAATTTTCCTGAAGCTTGCCATTCCTTCAGCTGTAATGGTCTGCTTGGAAATGTGGTCATTTGAATTAATGGTTCTCCTTTCTGGTCTTCTTCCAAATCCAAATTTAAATACAACTGCAGCTGTTTGGATGATCCCCTTCGGACTTAGTGGAGCTGTAAGCATTCGTGTTTCGAATGAACTTGGTGCTGGTCATCCACAGGCTGCGCGTTTGGCAGTTTGTGTCGTTGTAGTGATTGCCGTTATTGAGAGCATCTTAGTCGGAACGGTGATTATACTAGTTCGTAATTTATGGGGATATGCTTATAGCAACGAAGAAGAAGTAGTCAAATATGTTGCAACTATGATGCCAATTCTTGCCGTATCCCACTTACTAGATGGAATGCAAGCTGTTCTTTCAGGTACTGCTAGAGGATGTGGTTGGCAGAAAATTGGCGCATTTGTCAACCTTGGATCATATTATTTAGTTGGGATTCCAGCAGCCATTGTATTCGCTTTTGCACTGCATATTGGCGGGAAGGGCCTGTGGCTGGGGATTATATGTGCCCTCGTTGTTCAAGCGCTTTGTCTAATGATCATTACAATACGCACAGATTGGGAGAAAGAGGCAAAGAAGGCTACTGATAGAGTTCATAATGCGATAACAACAAAGAGCTTAGTCTCCTGA
Protein sequence

>Msa0997550

MSLHSPLIVEETKQNKEEERRRLVEEVKTQLWLSGPLISVTLLNYGINIISLMFVGHLGELSLSGASMATSFASVTGFSLLYRMSGIHMQRAMFILMTVAIPLAIIWANTRSILILLGQDHEISTEAGSYAKLMVPCLFAYGLLQCLNRFLQTQNILHCYNITSSSSMLDKWYTSSRGAAIANSISYWVNVTILALYVKFSPSCKKTWNGFSKEALALNNIPIFLKLAIPSAVMVCLEMWSFELMVLLSGLLPNPNLNTTAAVWMIPFGLSGAVSIRVSNELGAGHPQAARLAVCVVVVIAVIESILVGTVIILVRNLWGYAYSNEEEVVKYVATMMPILAVSHLLDGMQAVLSGTARGCGWQKIGAFVNLGSYYLVGIPAAIVFAFALHIGGKGLWLGIICALVVQALCLMIITIRTDWEKEAKKATDRVHNAITTKSLVS*