AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1388230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1388230 MtrunA17_Chr5g0401451 98.730 630 8 0 1 630 1 630 0.0 1286
Msa1388230 MtrunA17_Chr4g0053841 70.279 609 161 9 28 630 1 595 0.0 854
Msa1388230 MtrunA17_Chr4g0010091 41.694 614 316 8 6 606 1 585 1.31e-166 489
Msa1388230 MtrunA17_Chr1g0195911 41.784 639 300 17 25 630 22 621 9.44e-158 467
Msa1388230 MtrunA17_Chr7g0268781 40.469 640 315 14 25 630 22 629 1.50e-153 456
Msa1388230 MtrunA17_Chr5g0432861 39.094 596 297 7 24 606 9 551 6.82e-146 435
Msa1388230 MtrunA17_Chr3g0108331 40.681 617 300 12 24 630 8 568 4.09e-145 432
Msa1388230 MtrunA17_Chr7g0245201 41.531 614 311 17 35 630 57 640 9.09e-143 429
Msa1388230 MtrunA17_Chr4g0050021 38.356 584 289 8 29 551 33 606 5.59e-132 402
Msa1388230 MtrunA17_Chr2g0279481 36.780 590 298 7 28 551 32 612 3.45e-126 388
Msa1388230 MtrunA17_Chr2g0303541 38.278 546 305 11 34 551 20 561 5.96e-126 386
Msa1388230 MtrunA17_Chr8g0382231 36.269 579 301 11 10 551 19 566 6.43e-121 372
Msa1388230 MtrunA17_Chr2g0285511 35.636 550 299 10 34 536 20 561 1.14e-114 355
Msa1388230 MtrunA17_Chr2g0320691 35.043 585 341 13 24 576 7 584 4.41e-114 355
Msa1388230 MtrunA17_Chr8g0374011 34.522 617 326 14 24 567 1 612 2.94e-113 353
Msa1388230 MtrunA17_Chr1g0207461 38.205 479 259 11 37 493 25 488 2.50e-103 327
Msa1388230 MtrunA17_Chr1g0181181 35.306 490 269 7 34 499 20 485 2.96e-103 324
Msa1388230 MtrunA17_Chr5g0401341 38.936 470 264 9 37 493 24 483 2.77e-102 324
Msa1388230 MtrunA17_Chr1g0150191 38.136 472 268 11 36 491 23 486 5.88e-101 320
Msa1388230 MtrunA17_Chr5g0412591 37.972 503 232 10 36 493 22 489 2.94e-100 318
Msa1388230 MtrunA17_Chr1g0199631 36.674 469 260 7 58 500 8 465 5.95e-98 311
Msa1388230 MtrunA17_Chr4g0076521 35.408 466 281 7 36 493 22 475 2.50e-93 300
Msa1388230 MtrunA17_Chr8g0367891 35.433 508 270 12 43 519 29 509 4.93e-88 285
Msa1388230 MtrunA17_Chr2g0290741 35.000 460 275 7 39 493 25 465 2.95e-85 278
Msa1388230 MtrunA17_Chr1g0191711 30.996 542 323 12 26 554 44 547 1.37e-80 265
Msa1388230 MtrunA17_Chr1g0206621 69.343 137 40 1 47 181 75 211 3.45e-61 203
Msa1388230 MtrunA17_Chr4g0003891 39.362 282 152 4 219 494 46 314 1.75e-58 203
Msa1388230 MtrunA17_Chr2g0332281 36.119 335 187 6 219 544 24 340 2.28e-55 194
Msa1388230 MtrunA17_Chr4g0023801 27.405 551 331 19 43 558 11 527 3.38e-49 180
Msa1388230 MtrunA17_Chr4g0037661 25.185 540 345 15 43 551 6 517 4.32e-41 157
Msa1388230 MtrunA17_Chr1g0163311 25.801 562 329 20 43 562 6 521 2.22e-38 149
Msa1388230 MtrunA17_Chr2g0280541 25.799 407 250 13 107 495 2 374 3.02e-26 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1388230 AT5G67385.1 67.851 619 180 8 16 630 1 604 0.0 826
Msa1388230 AT5G67385.4 67.770 574 166 8 61 630 1 559 0.0 759
Msa1388230 AT5G67385.5 67.770 574 166 8 61 630 1 559 0.0 759
Msa1388230 AT5G67385.2 67.770 574 166 8 61 630 1 559 0.0 759
Msa1388230 AT5G67385.3 67.770 574 166 8 61 630 1 559 0.0 759
Msa1388230 AT3G49970.1 54.795 584 185 15 52 630 6 515 0.0 583
Msa1388230 AT3G49970.2 54.795 584 185 15 52 630 6 515 0.0 583
Msa1388230 AT5G48800.1 45.017 602 275 12 20 606 22 582 5.93e-164 483
Msa1388230 AT2G30520.1 40.514 622 313 10 24 630 14 593 3.77e-151 449
Msa1388230 AT1G03010.1 40.678 649 308 15 24 630 21 634 6.92e-150 447
Msa1388230 AT1G03010.2 40.852 634 299 14 39 630 8 607 1.29e-147 441
Msa1388230 AT1G03010.3 40.852 634 299 14 39 630 34 633 1.66e-147 441
Msa1388230 AT2G47860.1 42.986 556 270 12 25 551 23 560 1.13e-143 431
Msa1388230 AT2G47860.3 41.278 579 270 13 25 551 23 583 2.20e-138 419
Msa1388230 AT3G08570.1 42.589 533 278 13 35 551 30 550 3.88e-134 406
Msa1388230 AT2G30520.2 39.761 586 296 10 60 630 2 545 1.19e-132 400
Msa1388230 AT2G30520.3 39.761 586 296 10 60 630 2 545 1.19e-132 400
Msa1388230 AT1G30440.1 35.993 589 323 12 25 566 10 591 3.40e-120 372
Msa1388230 AT3G08660.1 37.914 604 298 14 35 626 30 568 8.71e-119 365
Msa1388230 AT5G13600.1 38.855 489 269 8 34 500 20 500 3.59e-115 357
Msa1388230 AT5G64330.2 35.823 589 274 9 30 516 42 628 3.02e-113 354
Msa1388230 AT5G64330.1 33.888 661 311 11 30 569 42 697 6.56e-113 355
Msa1388230 AT5G03250.1 35.522 594 330 16 25 577 10 591 1.75e-109 342
Msa1388230 AT3G08660.3 37.587 572 281 13 67 626 1 508 2.33e-109 339
Msa1388230 AT2G47860.4 43.379 438 211 10 25 442 23 443 5.51e-109 335
Msa1388230 AT5G66560.1 36.229 541 272 11 34 508 13 546 1.68e-106 336
Msa1388230 AT3G44820.1 33.333 585 339 10 24 563 7 585 1.79e-105 333
Msa1388230 AT2G47860.6 41.215 461 211 11 25 442 23 466 1.11e-103 323
Msa1388230 AT5G64330.3 35.159 566 263 9 53 516 6 569 2.17e-103 326
Msa1388230 AT2G47860.5 40.789 456 227 10 125 551 1 442 1.63e-101 319
Msa1388230 AT2G47860.2 40.789 456 227 10 125 551 1 442 1.63e-101 319
Msa1388230 AT5G66560.3 36.711 523 258 11 52 508 2 517 2.58e-101 322
Msa1388230 AT5G66560.4 40.312 449 239 10 82 508 66 507 6.01e-101 321
Msa1388230 AT2G14820.2 36.810 489 276 10 39 507 26 501 1.31e-100 320
Msa1388230 AT2G14820.1 36.810 489 276 10 39 507 26 501 1.31e-100 320
Msa1388230 AT1G67900.5 38.095 525 251 11 37 508 23 526 3.33e-99 316
Msa1388230 AT1G67900.1 38.095 525 251 11 37 508 23 526 3.33e-99 316
Msa1388230 AT1G67900.3 38.095 525 251 11 37 508 23 526 3.33e-99 316
Msa1388230 AT1G67900.6 38.095 525 251 11 37 508 23 526 3.33e-99 316
Msa1388230 AT1G67900.2 38.095 525 251 11 37 508 23 526 3.33e-99 316
Msa1388230 AT1G67900.4 38.095 525 251 11 37 508 23 526 3.33e-99 316
Msa1388230 AT3G50840.1 33.962 530 318 7 29 551 5 509 3.81e-98 311
Msa1388230 AT5G10250.2 33.391 578 313 13 23 562 33 576 5.02e-98 313
Msa1388230 AT5G10250.1 33.275 568 312 13 23 562 33 561 7.01e-96 307
Msa1388230 AT4G31820.1 35.477 482 275 11 39 508 26 483 3.33e-91 293
Msa1388230 AT4G37590.1 35.174 489 270 10 37 509 24 481 4.27e-88 286
Msa1388230 AT3G50840.2 34.137 498 296 7 61 551 1 473 1.11e-87 283
Msa1388230 AT3G50840.3 34.137 498 296 7 61 551 1 473 1.11e-87 283
Msa1388230 AT5G67440.3 35.714 462 283 9 37 493 24 476 2.58e-87 283
Msa1388230 AT5G67440.1 35.714 462 283 9 37 493 24 476 2.58e-87 283
Msa1388230 AT2G23050.1 33.824 476 269 10 37 506 24 459 3.09e-84 273
Msa1388230 AT5G47800.3 32.238 487 295 10 35 508 21 485 4.33e-83 272
Msa1388230 AT5G47800.1 32.238 487 295 10 35 508 21 485 4.33e-83 272
Msa1388230 AT5G47800.4 32.238 487 295 10 35 508 21 485 4.33e-83 272
Msa1388230 AT3G08660.2 39.474 418 219 10 35 446 30 419 1.35e-82 266
Msa1388230 AT5G67440.2 35.360 444 273 9 55 493 5 439 1.32e-81 267
Msa1388230 AT5G47800.2 32.238 487 295 10 35 508 134 598 1.83e-81 270
Msa1388230 AT4G31820.2 34.802 454 260 11 67 508 1 430 5.17e-80 263
Msa1388230 AT3G26490.1 32.685 514 287 12 37 504 23 523 1.07e-79 264
Msa1388230 AT3G26490.3 32.685 514 287 12 37 504 23 523 1.07e-79 264
Msa1388230 AT3G26490.4 32.685 514 287 12 37 504 23 523 1.07e-79 264
Msa1388230 AT5G03250.2 34.151 489 276 13 125 577 1 479 5.95e-76 251
Msa1388230 AT5G66560.2 33.122 474 245 10 34 442 13 479 1.81e-75 249
Msa1388230 AT3G26490.2 31.264 451 256 11 37 446 23 460 5.83e-60 209
Msa1388230 AT1G52770.1 36.624 314 165 5 193 494 13 304 2.21e-56 197
Msa1388230 AT2G23050.2 32.898 383 216 9 125 506 1 343 7.48e-56 194
Msa1388230 AT5G48130.1 28.390 472 287 12 34 491 33 467 4.33e-55 198
Msa1388230 AT3G15570.1 35.789 285 152 4 228 494 38 309 5.92e-51 183
Msa1388230 AT3G19850.1 27.669 459 276 10 65 491 29 463 1.27e-47 176
Msa1388230 AT1G50280.1 28.753 473 293 12 42 487 5 460 3.68e-46 171
Msa1388230 AT1G50280.2 29.977 427 261 10 82 487 18 427 1.88e-45 169
Msa1388230 AT3G22104.1 27.193 456 272 10 42 478 6 420 5.20e-40 154
Msa1388230 AT3G03510.1 27.696 408 239 9 94 496 8 364 4.13e-38 147
Msa1388230 AT5G17580.2 25.745 470 307 10 42 498 22 462 1.19e-37 147
Msa1388230 AT5G17580.1 25.745 470 307 10 42 498 7 447 1.68e-37 147
Msa1388230 AT3G22104.4 25.587 383 228 7 112 478 4 345 9.44e-28 117
Msa1388230 AT3G22104.2 25.587 383 228 7 112 478 4 345 9.44e-28 117
Msa1388230 AT3G22104.3 25.587 383 228 7 112 478 4 345 9.44e-28 117
Msa1388230 AT3G49900.2 31.795 195 112 7 30 213 55 239 1.30e-13 74.3
Msa1388230 AT3G49900.1 31.250 192 117 6 30 213 55 239 1.47e-13 73.9

Find 135 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTCCATGTCACTTAAAT+TGG 0.099646 tig0021037:+76022 None:intergenic
AGCCTTAGACATCTATTTAA+AGG 0.193457 tig0021037:-76880 Msa1388230:intron
TCTTTCGAGCAGGATATATT+TGG 0.214701 tig0021037:-77407 Msa1388230:intron
TAAAGGAAGATTCTCCAATT+TGG 0.228988 tig0021037:+78930 None:intergenic
GACATTGCATTCTTCTCTTT+TGG 0.249323 tig0021037:+77308 None:intergenic
GGAAACCACTTTGTCTATAA+TGG 0.269644 tig0021037:-77074 Msa1388230:CDS
CACCACCACCCTTTACAATA+TGG 0.282070 tig0021037:+75362 None:intergenic
GGTTTCCAATTTGAGATTCT+TGG 0.291899 tig0021037:+77090 None:intergenic
CTAGATAAGAAGACCTTGTT+TGG 0.292025 tig0021037:-79496 Msa1388230:five_prime_UTR
ATAACTTAAGAAAATGTTAA+AGG 0.301852 tig0021037:-79321 Msa1388230:five_prime_UTR
ATTCCTTCGTATTCATTTAC+CGG 0.307235 tig0021037:-77161 Msa1388230:CDS
TACCTTTAAATAGATGTCTA+AGG 0.307550 tig0021037:+76878 None:intergenic
AGTTGCTTGCAGGCTTGATT+TGG 0.310330 tig0021037:-77234 Msa1388230:CDS
TTTAGTTTGGTGTGAATAGA+AGG 0.321571 tig0021037:+75837 None:intergenic
CTATAAGAACTCTTTGAAAA+AGG 0.323564 tig0021037:+78049 None:intergenic
AATAGCAGAGAAAACAAAAT+TGG 0.326409 tig0021037:-78241 Msa1388230:CDS
TTGATACAACAGTTGCTTGC+AGG 0.331579 tig0021037:-77244 Msa1388230:CDS
GTTGAACTCTCTGATGTTCC+TGG 0.333752 tig0021037:-78492 Msa1388230:CDS
ACCTGAATATACATTTAGTT+TGG 0.337497 tig0021037:+75824 None:intergenic
AAAAGAATGGCAATGCAATT+AGG 0.350908 tig0021037:-77209 Msa1388230:CDS
TCGAAAATCATTTATAAGTT+CGG 0.354375 tig0021037:-75592 Msa1388230:CDS
AATTACCAAGAATCTCAAAT+TGG 0.354888 tig0021037:-77095 Msa1388230:CDS
AGTGATGTCAATGTCCAAAT+TGG 0.359152 tig0021037:-78944 Msa1388230:CDS
CTCTCCATGTCACTTAAATT+GGG 0.374237 tig0021037:+76023 None:intergenic
TTCTGTCATCTCAAGATATT+CGG 0.376339 tig0021037:+78377 None:intergenic
TCACAAGGTCAAATATTTGT+TGG 0.378136 tig0021037:-79468 Msa1388230:five_prime_UTR
CTAGAAGAAAGGTAATTCTA+TGG 0.387879 tig0021037:+79515 None:intergenic
ATAGAAATTTATTTGATTTG+AGG 0.387896 tig0021037:-79434 Msa1388230:five_prime_UTR
ATAAAATATAATTCTTGAGT+TGG 0.390624 tig0021037:-75335 Msa1388230:three_prime_UTR
AAGGAATCTTCAAATATAAA+AGG 0.394765 tig0021037:-79302 Msa1388230:five_prime_UTR
AAAGAAAGTTTGCAGCATAA+TGG 0.400198 tig0021037:-76000 Msa1388230:CDS
AGATTATTCAGTTGGGAACT+TGG 0.401252 tig0021037:-78355 Msa1388230:CDS
GGATTGCATTGCAACCTTGC+TGG 0.406623 tig0021037:-75314 Msa1388230:three_prime_UTR
TATCCACACTTGGAGACTAA+TGG 0.409668 tig0021037:+78561 None:intergenic
AGAGAACTTGGACAACGGTT+TGG 0.419568 tig0021037:+75916 None:intergenic
AACAGAGACCAGTTGTTGAT+TGG 0.421313 tig0021037:-78104 Msa1388230:CDS
AGTTCGGTTTCAAAGAAACT+TGG 0.421844 tig0021037:-75576 Msa1388230:CDS
AGTGGGAACCATATTGTAAA+GGG 0.423439 tig0021037:-75370 Msa1388230:three_prime_UTR
GGGTTTAAACAGTTTGCTAT+TGG 0.424370 tig0021037:-78012 Msa1388230:CDS
AAGAGTTCTTATAGCAATGA+TGG 0.430446 tig0021037:-78040 Msa1388230:CDS
AAATGAAGACCTCAAATTAG+AGG 0.432193 tig0021037:-75760 Msa1388230:CDS
CGACTTCGCGATGCAATGAA+TGG 0.432759 tig0021037:-75879 Msa1388230:CDS
ATCTTCCTTTACTTTACATA+AGG 0.434540 tig0021037:-78918 Msa1388230:intron
ATGACAGAAGATTATTCAGT+TGG 0.439432 tig0021037:-78363 Msa1388230:CDS
CAATCAAAACCTACAGAAGA+TGG 0.439804 tig0021037:-76912 Msa1388230:CDS
TGAACGGGTTGGAAAGCTAA+TGG 0.443135 tig0021037:-77015 Msa1388230:CDS
GAAGGTGAAGCATTTGCAAT+AGG 0.443580 tig0021037:+75648 None:intergenic
GATGGTATTGTTACTGCTAA+AGG 0.444847 tig0021037:-75528 Msa1388230:CDS
TAGATAAGAAGACCTTGTTT+GGG 0.445988 tig0021037:-79495 Msa1388230:five_prime_UTR
CAACGGTTTGGACTGGTAAC+CGG 0.447250 tig0021037:+75928 None:intergenic
ACTTCACTACCTCTAATTTG+AGG 0.447292 tig0021037:+75751 None:intergenic
ACAGAAATCTCTACGAGGTT+TGG 0.458956 tig0021037:-77968 Msa1388230:intron
ATTTGGACATTGACATCACT+TGG 0.463936 tig0021037:+78947 None:intergenic
AATTGCTTATATTGCTTGCA+AGG 0.465206 tig0021037:-78199 Msa1388230:CDS
TCAGCCCACCAATCAACAAC+TGG 0.478880 tig0021037:+78096 None:intergenic
CACGTTATTCGATGTGGACA+CGG 0.493140 tig0021037:-77135 Msa1388230:CDS
TTCCTTCGTATTCATTTACC+GGG 0.494561 tig0021037:-77160 Msa1388230:CDS
AAGTGGGAACCATATTGTAA+AGG 0.496550 tig0021037:-75371 Msa1388230:three_prime_UTR
CCTTGTGAATTTCCCAAACA+AGG 0.497170 tig0021037:+79483 None:intergenic
CTATACATCCCATCTTCTGT+AGG 0.497214 tig0021037:+76903 None:intergenic
ATACCATCCAAACGTGGAAA+AGG 0.497451 tig0021037:+75543 None:intergenic
ACCCGTTCAATATCACTTTG+TGG 0.498785 tig0021037:+77029 None:intergenic
ACCACAAAGTGATATTGAAC+GGG 0.498903 tig0021037:-77030 Msa1388230:CDS
TGTGCATAGAGCATTAGAAT+AGG 0.499783 tig0021037:+77988 None:intergenic
AAGGTCATGAAAATAAATAC+AGG 0.501104 tig0021037:-75464 Msa1388230:three_prime_UTR
ATGTCATCACGATGTTAAAG+TGG 0.506615 tig0021037:-75388 Msa1388230:three_prime_UTR
TCTCCAAGTGTGGATACATT+CGG 0.506959 tig0021037:-78554 Msa1388230:CDS
CGAGCAGGATATATTTGGAA+AGG 0.507201 tig0021037:-77402 Msa1388230:intron
CACTACCTTATGTAAAGTAA+AGG 0.508799 tig0021037:+78913 None:intergenic
GCTTGATTTGGAGAAAAGAA+TGG 0.510077 tig0021037:-77222 Msa1388230:CDS
ACCAAACTAAATGTATATTC+AGG 0.522428 tig0021037:-75825 Msa1388230:CDS
GAGTGTCGTCGATTCTTGCT+TGG 0.523000 tig0021037:+75495 None:intergenic
AGAGACCAGTTGTTGATTGG+TGG 0.523518 tig0021037:-78101 Msa1388230:CDS
GAGCAGGATATATTTGGAAA+GGG 0.526552 tig0021037:-77401 Msa1388230:intron
AGTGTACCGTATGCTTAGAT+AGG 0.529667 tig0021037:+75212 None:intergenic
TGACAGAAGATTATTCAGTT+GGG 0.532521 tig0021037:-78362 Msa1388230:CDS
GGTCTTCTTATCTAGAAGAA+AGG 0.533076 tig0021037:+79504 None:intergenic
AAATCTTTCTGTACTTGCCA+TGG 0.535061 tig0021037:-79217 Msa1388230:exon
GCTGTTCGTAATAGAGAACT+TGG 0.535435 tig0021037:+75904 None:intergenic
AACTGATAAATTTCTGTCAG+TGG 0.538405 tig0021037:+76971 None:intergenic
CACCACAAAGTGATATTGAA+CGG 0.544352 tig0021037:-77031 Msa1388230:CDS
ACTTGGACAACGGTTTGGAC+TGG 0.546671 tig0021037:+75921 None:intergenic
TTTCTGCTTCATTTATAGTG+AGG 0.547211 tig0021037:-79243 Msa1388230:five_prime_UTR
GTAATAGAGAACTTGGACAA+CGG 0.549721 tig0021037:+75911 None:intergenic
TTTCCGAATGTATCCACACT+TGG 0.551161 tig0021037:+78551 None:intergenic
AGAAGTGATAGCGGCTCTGA+CGG 0.551480 tig0021037:-78153 Msa1388230:CDS
GTTCGGTTTCAAAGAAACTT+GGG 0.553017 tig0021037:-75575 Msa1388230:CDS
AGGATATATTTGGAAAGGGA+AGG 0.553677 tig0021037:-77397 Msa1388230:CDS
TCTTTCTGTACTTGCCATGG+TGG 0.556166 tig0021037:-79214 Msa1388230:exon
CCTTGTTTGGGAAATTCACA+AGG 0.561007 tig0021037:-79483 Msa1388230:five_prime_UTR
CGACGACACTCTATTTCCTA+AGG 0.562175 tig0021037:-75483 Msa1388230:exon
AACCATATTGTAAAGGGTGG+TGG 0.564776 tig0021037:-75364 Msa1388230:three_prime_UTR
TTATTCAGTTGGGAACTTGG+TGG 0.567121 tig0021037:-78352 Msa1388230:CDS
CATCACCATTATAGACAAAG+TGG 0.567160 tig0021037:+77069 None:intergenic
CAAAGTGATATTGAACGGGT+TGG 0.567740 tig0021037:-77026 Msa1388230:CDS
GAAGAAAGGTAATTCTATGG+AGG 0.574630 tig0021037:+79518 None:intergenic
TATAAATTTCGAGATAAATG+TGG 0.578053 tig0021037:-78427 Msa1388230:CDS
TAAATCAACTGTTAAATCAA+CGG 0.578589 tig0021037:-75691 Msa1388230:CDS
CACATGCAGCTCAAAACGAC+CGG 0.579581 tig0021037:-75947 Msa1388230:CDS
TCGATGTGGACACGGTTCAA+AGG 0.579585 tig0021037:-77127 Msa1388230:CDS
TCAGTTATAGTATGTGCTCA+AGG 0.582768 tig0021037:+79278 None:intergenic
ATTCTATGGAGGGTAGATGA+AGG 0.584535 tig0021037:+79529 None:intergenic
CTTATAGCAATGATGGCTAG+AGG 0.584871 tig0021037:-78033 Msa1388230:CDS
CTATGAAAAGAACAAGTGAC+TGG 0.585425 tig0021037:-79119 Msa1388230:intron
TTATAGCAATGATGGCTAGA+GGG 0.589083 tig0021037:-78032 Msa1388230:CDS
AATCAAAACCTACAGAAGAT+GGG 0.590751 tig0021037:-76911 Msa1388230:CDS
CTAGCAGCAAAATTCTGCTA+CGG 0.592687 tig0021037:-78450 Msa1388230:CDS
TCAAATGCTTCTGCACCACC+AGG 0.593786 tig0021037:+78474 None:intergenic
AAGAAAGGTAATTCTATGGA+GGG 0.598694 tig0021037:+79519 None:intergenic
CACATCGAATAACGTGTCCC+CGG 0.613083 tig0021037:+77142 None:intergenic
CGGGGACACGTTATTCGATG+TGG 0.614759 tig0021037:-77141 Msa1388230:CDS
TCATCCCAATTTAAGTGACA+TGG 0.614869 tig0021037:-76027 Msa1388230:CDS
GGTAGATGAAGGAATATGAG+TGG 0.620727 tig0021037:+79540 None:intergenic
TAAATCAACGGTAAAATCAA+CGG 0.622532 tig0021037:-75679 Msa1388230:CDS
TCAGCAGAATGAGAATGAGA+AGG 0.624756 tig0021037:+75630 None:intergenic
CATCGCTATGCTTAGATGTG+TGG 0.625147 tig0021037:-78400 Msa1388230:CDS
GAGACCAGTTGTTGATTGGT+GGG 0.625912 tig0021037:-78100 Msa1388230:CDS
GTCTAGACCACGATCCACCA+TGG 0.633131 tig0021037:+79200 None:intergenic
TGTCATCACGATGTTAAAGT+GGG 0.635930 tig0021037:-75387 Msa1388230:three_prime_UTR
TTTAACAGTTGATTTAAGCG+TGG 0.636314 tig0021037:+75698 None:intergenic
CGTTCAATATCACTTTGTGG+TGG 0.641780 tig0021037:+77032 None:intergenic
TATCCATTAGTCTCCAAGTG+TGG 0.642032 tig0021037:-78564 Msa1388230:CDS
GTATTGTTACTGCTAAAGGT+AGG 0.645750 tig0021037:-75524 Msa1388230:CDS
GGGAACCATATTGTAAAGGG+TGG 0.650201 tig0021037:-75367 Msa1388230:three_prime_UTR
GTACTTGCCATGGTGGATCG+TGG 0.650434 tig0021037:-79207 Msa1388230:exon
TATATTGCGAGAAGTACAAG+TGG 0.662671 tig0021037:-75278 Msa1388230:three_prime_UTR
AACATCAGAGAGTTCAACGA+AGG 0.692508 tig0021037:+78497 None:intergenic
ATGAGAGTAGTACACCAGCA+AGG 0.699607 tig0021037:+75300 None:intergenic
GAACTCTCTGATGTTCCTGG+TGG 0.699693 tig0021037:-78489 Msa1388230:CDS
GAGTTCAACGAAGGACACGT+CGG 0.700242 tig0021037:+78506 None:intergenic
TCATCTGTAAGAAGTGATAG+CGG 0.715394 tig0021037:-78162 Msa1388230:CDS
TCCCCGGTAAATGAATACGA+AGG 0.717871 tig0021037:+77158 None:intergenic
GTAACAATACCATCCAAACG+TGG 0.725647 tig0021037:+75537 None:intergenic
TCCTTCGTATTCATTTACCG+GGG 0.730046 tig0021037:-77159 Msa1388230:CDS
TATGCACAGAAATCTCTACG+AGG 0.833179 tig0021037:-77973 Msa1388230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAATTATTAAGAATTA+AGG + tig0021037:77310-77329 None:intergenic 10.0%
!!! ATAGAAATTTATTTGATTTG+AGG - tig0021037:75305-75324 Msa1388230:three_prime_UTR 15.0%
!! ATAACTTAAGAAAATGTTAA+AGG - tig0021037:75418-75437 Msa1388230:three_prime_UTR 15.0%
!! CAAAAAAATCTTTATATTGA+CGG - tig0021037:76127-76146 Msa1388230:intron 15.0%
!!! GTTAAAATTTTACAATTTTC+AGG - tig0021037:78688-78707 Msa1388230:intron 15.0%
!! ATAAAATATAATTCTTGAGT+TGG - tig0021037:79404-79423 Msa1388230:five_prime_UTR 15.0%
!! AAGGAATCTTCAAATATAAA+AGG - tig0021037:75437-75456 Msa1388230:three_prime_UTR 20.0%
!!! TAATTATAGGATTTTTTCTC+AGG - tig0021037:75764-75783 Msa1388230:CDS 20.0%
!! TTAATCAATCATAAAAACGT+AGG + tig0021037:76102-76121 None:intergenic 20.0%
!! TATAAATTTCGAGATAAATG+TGG - tig0021037:76312-76331 Msa1388230:intron 20.0%
!!! TTTCTATCTTTTTTTTGAGA+GGG + tig0021037:76803-76822 None:intergenic 20.0%
!!! TTTTCTATCTTTTTTTTGAG+AGG + tig0021037:76804-76823 None:intergenic 20.0%
!! TTAAAAATAAATACTCCATC+CGG - tig0021037:76825-76844 Msa1388230:intron 20.0%
!! TATAAAAGTCTAATCAATGT+TGG + tig0021037:77239-77258 None:intergenic 20.0%
!!! TAGTAGTTTTAAAATGGATT+TGG + tig0021037:78047-78066 None:intergenic 20.0%
!!! TCTAAGTAGTAGTTTTAAAA+TGG + tig0021037:78053-78072 None:intergenic 20.0%
!!! TCCTATCATTAAATAGATTT+TGG - tig0021037:78197-78216 Msa1388230:CDS 20.0%
!! TTAACAAAATCAAAAGATCA+TGG - tig0021037:78449-78468 Msa1388230:CDS 20.0%
!! TAAATCAACTGTTAAATCAA+CGG - tig0021037:79048-79067 Msa1388230:intron 20.0%
!! TCGAAAATCATTTATAAGTT+CGG - tig0021037:79147-79166 Msa1388230:CDS 20.0%
!!! CATCTATTTTTTTTGTTTGT+GGG + tig0021037:79494-79513 None:intergenic 20.0%
!!! TCATCTATTTTTTTTGTTTG+TGG + tig0021037:79495-79514 None:intergenic 20.0%
!!! AAAAGTTTTAGGGAATAATG+AGG + tig0021037:75352-75371 None:intergenic 25.0%
! AATTAAGCAATACAACTCAT+TGG + tig0021037:75749-75768 None:intergenic 25.0%
! GAGTTGTATTGCTTAATTAT+AGG - tig0021037:75751-75770 Msa1388230:CDS 25.0%
! ATCTTCCTTTACTTTACATA+AGG - tig0021037:75821-75840 Msa1388230:CDS 25.0%
! ATCATAAAAACGTAGGTAAA+AGG + tig0021037:76095-76114 None:intergenic 25.0%
!!! AATTTTGTTTTCTCTGCTAT+TGG + tig0021037:76499-76518 None:intergenic 25.0%
! AATAGCAGAGAAAACAAAAT+TGG - tig0021037:76498-76517 Msa1388230:intron 25.0%
!! CTATAAGAACTCTTTGAAAA+AGG + tig0021037:76693-76712 None:intergenic 25.0%
!!! TTCTATCTTTTTTTTGAGAG+GGG + tig0021037:76802-76821 None:intergenic 25.0%
!! AATTTGCTTATAATAGTGAC+CGG + tig0021037:76956-76975 None:intergenic 25.0%
! CATTCAATAAATGATGTATG+TGG - tig0021037:76882-76901 Msa1388230:CDS 25.0%
! CATTCAATAAATGATGTATG+TGG + tig0021037:76882-76901 None:intergenic 25.0%
!! AATTTGCTTATAATAGTGAC+CGG - tig0021037:76956-76975 Msa1388230:CDS 25.0%
! TGTAATTATATGTGTAAGTG+CGG + tig0021037:77078-77097 None:intergenic 25.0%
! AATTACCAAGAATCTCAAAT+TGG - tig0021037:77644-77663 Msa1388230:intron 25.0%
! TACCTTTAAATAGATGTCTA+AGG + tig0021037:77864-77883 None:intergenic 25.0%
! ATCTATTTACATACAGAGAT+AGG + tig0021037:78108-78127 None:intergenic 25.0%
! GCCAAAATCTATTTAATGAT+AGG + tig0021037:78201-78220 None:intergenic 25.0%
! AGCTAAAAATCTTTATCCAA+TGG - tig0021037:78237-78256 Msa1388230:CDS 25.0%
!! CAAATAAATGTATTGCTGTT+TGG - tig0021037:78413-78432 Msa1388230:CDS 25.0%
! TTTGTATAGTAACACATGTT+TGG + tig0021037:78550-78569 None:intergenic 25.0%
! ACAATAATACATGTTTGGAA+TGG + tig0021037:78586-78605 None:intergenic 25.0%
! ACACTACAATAATACATGTT+TGG + tig0021037:78591-78610 None:intergenic 25.0%
! ACCAAACTAAATGTATATTC+AGG - tig0021037:78914-78933 Msa1388230:intron 25.0%
! ACCTGAATATACATTTAGTT+TGG + tig0021037:78918-78937 None:intergenic 25.0%
! TAAATCAACGGTAAAATCAA+CGG - tig0021037:79060-79079 Msa1388230:intron 25.0%
!! GAACTTATAAATGATTTTCG+AGG + tig0021037:79148-79167 None:intergenic 25.0%
!!! GTATTTATTTTCATGACCTT+AGG + tig0021037:79275-79294 None:intergenic 25.0%
! AAGGTCATGAAAATAAATAC+AGG - tig0021037:79275-79294 Msa1388230:five_prime_UTR 25.0%
AAGAAAGGTAATTCTATGGA+GGG + tig0021037:75223-75242 None:intergenic 30.0%
CTAGAAGAAAGGTAATTCTA+TGG + tig0021037:75227-75246 None:intergenic 30.0%
! TAGATAAGAAGACCTTGTTT+GGG - tig0021037:75244-75263 Msa1388230:three_prime_UTR 30.0%
TCACAAGGTCAAATATTTGT+TGG - tig0021037:75271-75290 Msa1388230:three_prime_UTR 30.0%
TTTCTGCTTCATTTATAGTG+AGG - tig0021037:75496-75515 Msa1388230:CDS 30.0%
!! TATTGTTTGCTACCTTTTGT+TGG - tig0021037:75708-75727 Msa1388230:CDS 30.0%
TAAAGGAAGATTCTCCAATT+TGG + tig0021037:75812-75831 None:intergenic 30.0%
CACTACCTTATGTAAAGTAA+AGG + tig0021037:75829-75848 None:intergenic 30.0%
TTGAAAGCAAGATGTATATG+CGG - tig0021037:76021-76040 Msa1388230:CDS 30.0%
TTCTGTCATCTCAAGATATT+CGG + tig0021037:76365-76384 None:intergenic 30.0%
ATGACAGAAGATTATTCAGT+TGG - tig0021037:76376-76395 Msa1388230:intron 30.0%
TGACAGAAGATTATTCAGTT+GGG - tig0021037:76377-76396 Msa1388230:intron 30.0%
AATTGCTTATATTGCTTGCA+AGG - tig0021037:76540-76559 Msa1388230:intron 30.0%
AAGAGTTCTTATAGCAATGA+TGG - tig0021037:76699-76718 Msa1388230:intron 30.0%
GTAATTATATGTGTAAGTGC+GGG + tig0021037:77077-77096 None:intergenic 30.0%
!!! ATTTTTTCTTTCTTTCGAGC+AGG - tig0021037:77322-77341 Msa1388230:CDS 30.0%
! AAGACAAGAGCATGAAAAAA+GGG - tig0021037:77379-77398 Msa1388230:CDS 30.0%
!! CATTCTTCTCTTTTGGTAAT+AGG + tig0021037:77427-77446 None:intergenic 30.0%
AAAAGAATGGCAATGCAATT+AGG - tig0021037:77530-77549 Msa1388230:intron 30.0%
ATTCCTTCGTATTCATTTAC+CGG - tig0021037:77578-77597 Msa1388230:intron 30.0%
AACTGATAAATTTCTGTCAG+TGG + tig0021037:77771-77790 None:intergenic 30.0%
AATCAAAACCTACAGAAGAT+GGG - tig0021037:77828-77847 Msa1388230:intron 30.0%
AGCCTTAGACATCTATTTAA+AGG - tig0021037:77859-77878 Msa1388230:intron 30.0%
TAATGATAGGAAAACACAAG+CGG + tig0021037:78188-78207 None:intergenic 30.0%
!! AAGCTCTAGAGAATTAGTTT+AGG + tig0021037:78366-78385 None:intergenic 30.0%
ACAAAATCAAAAGATCATGG+TGG - tig0021037:78452-78471 Msa1388230:CDS 30.0%
!!! TGTGAAGTTTGTGTTGTTTT+TGG - tig0021037:78486-78505 Msa1388230:CDS 30.0%
AAAGAAAGTTTGCAGCATAA+TGG - tig0021037:78739-78758 Msa1388230:intron 30.0%
!! TTTAGTTTGGTGTGAATAGA+AGG + tig0021037:78905-78924 None:intergenic 30.0%
AAATGAAGACCTCAAATTAG+AGG - tig0021037:78979-78998 Msa1388230:intron 30.0%
! TTTAACAGTTGATTTAAGCG+TGG + tig0021037:79044-79063 None:intergenic 30.0%
!! ATAAATGATTTTCGAGGTAG+TGG + tig0021037:79142-79161 None:intergenic 30.0%
! TTTTCTCCTATCTAAGCATA+CGG - tig0021037:79521-79540 Msa1388230:five_prime_UTR 30.0%
GAAGAAAGGTAATTCTATGG+AGG + tig0021037:75224-75243 None:intergenic 35.0%
GGTCTTCTTATCTAGAAGAA+AGG + tig0021037:75238-75257 None:intergenic 35.0%
CTAGATAAGAAGACCTTGTT+TGG - tig0021037:75243-75262 Msa1388230:three_prime_UTR 35.0%
! TGCTGAGCTGAAAAGTTTTA+GGG + tig0021037:75362-75381 None:intergenic 35.0%
! ATGCTGAGCTGAAAAGTTTT+AGG + tig0021037:75363-75382 None:intergenic 35.0%
TCAGTTATAGTATGTGCTCA+AGG + tig0021037:75464-75483 None:intergenic 35.0%
AAATCTTTCTGTACTTGCCA+TGG - tig0021037:75522-75541 Msa1388230:CDS 35.0%
CTATGAAAAGAACAAGTGAC+TGG - tig0021037:75620-75639 Msa1388230:CDS 35.0%
! TGCTACCTTTTGTTGGATTT+AGG - tig0021037:75715-75734 Msa1388230:CDS 35.0%
! GCTACCTTTTGTTGGATTTA+GGG - tig0021037:75716-75735 Msa1388230:CDS 35.0%
ATTTGGACATTGACATCACT+TGG + tig0021037:75795-75814 None:intergenic 35.0%
AGTGATGTCAATGTCCAAAT+TGG - tig0021037:75795-75814 Msa1388230:CDS 35.0%
AGATTATTCAGTTGGGAACT+TGG - tig0021037:76384-76403 Msa1388230:intron 35.0%
GTAGCACTAAAAACAATTGC+AGG - tig0021037:76436-76455 Msa1388230:intron 35.0%
! TTTTCTCTGCTATTGGAAGA+AGG + tig0021037:76492-76511 None:intergenic 35.0%
TCATCTGTAAGAAGTGATAG+CGG - tig0021037:76577-76596 Msa1388230:intron 35.0%
TTATAGCAATGATGGCTAGA+GGG - tig0021037:76707-76726 Msa1388230:intron 35.0%
!! GGGTTTAAACAGTTTGCTAT+TGG - tig0021037:76727-76746 Msa1388230:intron 35.0%
! TGTGCATAGAGCATTAGAAT+AGG + tig0021037:76754-76773 None:intergenic 35.0%
! CTATAAAAGCATACTCCTTC+CGG + tig0021037:76978-76997 None:intergenic 35.0%
TGTTCATATCTGTGCTTCAT+AGG - tig0021037:77132-77151 Msa1388230:CDS 35.0%
! CTTCTTTTCTTTCGCAAACA+AGG + tig0021037:77167-77186 None:intergenic 35.0%
TCTTTCGAGCAGGATATATT+TGG - tig0021037:77332-77351 Msa1388230:CDS 35.0%
GAGCAGGATATATTTGGAAA+GGG - tig0021037:77338-77357 Msa1388230:CDS 35.0%
AGGATATATTTGGAAAGGGA+AGG - tig0021037:77342-77361 Msa1388230:CDS 35.0%
!!! CTTTTTTCATGCTCTTGTCT+TGG + tig0021037:77380-77399 None:intergenic 35.0%
! CAAGACAAGAGCATGAAAAA+AGG - tig0021037:77378-77397 Msa1388230:CDS 35.0%
! GACATTGCATTCTTCTCTTT+TGG + tig0021037:77434-77453 None:intergenic 35.0%
!! GCTTGATTTGGAGAAAAGAA+TGG - tig0021037:77517-77536 Msa1388230:intron 35.0%
TTCCTTCGTATTCATTTACC+GGG - tig0021037:77579-77598 Msa1388230:intron 35.0%
! GGTTTCCAATTTGAGATTCT+TGG + tig0021037:77652-77671 None:intergenic 35.0%
GGAAACCACTTTGTCTATAA+TGG - tig0021037:77665-77684 Msa1388230:intron 35.0%
CATCACCATTATAGACAAAG+TGG + tig0021037:77673-77692 None:intergenic 35.0%
CACCACAAAGTGATATTGAA+CGG - tig0021037:77708-77727 Msa1388230:intron 35.0%
ACCACAAAGTGATATTGAAC+GGG - tig0021037:77709-77728 Msa1388230:intron 35.0%
!!! TCTGTAGGTTTTGATTGTTC+AGG + tig0021037:77824-77843 None:intergenic 35.0%
CAATCAAAACCTACAGAAGA+TGG - tig0021037:77827-77846 Msa1388230:intron 35.0%
TCATCCCAATTTAAGTGACA+TGG - tig0021037:78712-78731 Msa1388230:intron 35.0%
! CTCTCCATGTCACTTAAATT+GGG + tig0021037:78719-78738 None:intergenic 35.0%
! TCTCTCCATGTCACTTAAAT+TGG + tig0021037:78720-78739 None:intergenic 35.0%
GTAATAGAGAACTTGGACAA+CGG + tig0021037:78831-78850 None:intergenic 35.0%
ACTTCACTACCTCTAATTTG+AGG + tig0021037:78991-79010 None:intergenic 35.0%
AGTTCGGTTTCAAAGAAACT+TGG - tig0021037:79163-79182 Msa1388230:CDS 35.0%
GTTCGGTTTCAAAGAAACTT+GGG - tig0021037:79164-79183 Msa1388230:CDS 35.0%
GATGGTATTGTTACTGCTAA+AGG - tig0021037:79211-79230 Msa1388230:exon 35.0%
GTATTGTTACTGCTAAAGGT+AGG - tig0021037:79215-79234 Msa1388230:exon 35.0%
ATGTCATCACGATGTTAAAG+TGG - tig0021037:79351-79370 Msa1388230:five_prime_UTR 35.0%
TGTCATCACGATGTTAAAGT+GGG - tig0021037:79352-79371 Msa1388230:five_prime_UTR 35.0%
AAGTGGGAACCATATTGTAA+AGG - tig0021037:79368-79387 Msa1388230:five_prime_UTR 35.0%
AGTGGGAACCATATTGTAAA+GGG - tig0021037:79369-79388 Msa1388230:five_prime_UTR 35.0%
! TATATTGCGAGAAGTACAAG+TGG - tig0021037:79461-79480 Msa1388230:five_prime_UTR 35.0%
ATTCTATGGAGGGTAGATGA+AGG + tig0021037:75213-75232 None:intergenic 40.0%
CCTTGTGAATTTCCCAAACA+AGG + tig0021037:75259-75278 None:intergenic 40.0%
! CCTTGTTTGGGAAATTCACA+AGG - tig0021037:75256-75275 Msa1388230:three_prime_UTR 40.0%
CAGTCCCTAAATCCAACAAA+AGG + tig0021037:75723-75742 None:intergenic 40.0%
! TATCCATTAGTCTCCAAGTG+TGG - tig0021037:76175-76194 Msa1388230:intron 40.0%
TATCCACACTTGGAGACTAA+TGG + tig0021037:76181-76200 None:intergenic 40.0%
TCTCCAAGTGTGGATACATT+CGG - tig0021037:76185-76204 Msa1388230:intron 40.0%
TTTCCGAATGTATCCACACT+TGG + tig0021037:76191-76210 None:intergenic 40.0%
AACATCAGAGAGTTCAACGA+AGG + tig0021037:76245-76264 None:intergenic 40.0%
CTAGCAGCAAAATTCTGCTA+CGG - tig0021037:76289-76308 Msa1388230:intron 40.0%
TTATTCAGTTGGGAACTTGG+TGG - tig0021037:76387-76406 Msa1388230:intron 40.0%
AACAGAGACCAGTTGTTGAT+TGG - tig0021037:76635-76654 Msa1388230:intron 40.0%
CTTATAGCAATGATGGCTAG+AGG - tig0021037:76706-76725 Msa1388230:intron 40.0%
TATGCACAGAAATCTCTACG+AGG - tig0021037:76766-76785 Msa1388230:intron 40.0%
ACAGAAATCTCTACGAGGTT+TGG - tig0021037:76771-76790 Msa1388230:intron 40.0%
TGCTTATAATAGTGACCGGA+TGG + tig0021037:76960-76979 None:intergenic 40.0%
TGCTTATAATAGTGACCGGA+AGG - tig0021037:76960-76979 Msa1388230:CDS 40.0%
AGACACAACTTGTGTAAGTG+CGG + tig0021037:77050-77069 None:intergenic 40.0%
CGAGCAGGATATATTTGGAA+AGG - tig0021037:77337-77356 Msa1388230:CDS 40.0%
TTGATACAACAGTTGCTTGC+AGG - tig0021037:77495-77514 Msa1388230:intron 40.0%
TCCTTCGTATTCATTTACCG+GGG - tig0021037:77580-77599 Msa1388230:intron 40.0%
CGTTCAATATCACTTTGTGG+TGG + tig0021037:77710-77729 None:intergenic 40.0%
ACCCGTTCAATATCACTTTG+TGG + tig0021037:77713-77732 None:intergenic 40.0%
CAAAGTGATATTGAACGGGT+TGG - tig0021037:77713-77732 Msa1388230:intron 40.0%
CTATACATCCCATCTTCTGT+AGG + tig0021037:77839-77858 None:intergenic 40.0%
!! CATAGAGGAAGTGTAGCTTT+TGG + tig0021037:77960-77979 None:intergenic 40.0%
ATGCAATCACAGATCTCAGT+CGG - tig0021037:77989-78008 Msa1388230:CDS 40.0%
GATCTCAGTCGGATGAAAAT+TGG - tig0021037:78000-78019 Msa1388230:CDS 40.0%
CTCGACCATTGAATGAAGAT+CGG - tig0021037:78161-78180 Msa1388230:CDS 40.0%
CACTTACGAATCTCTACCAT+TGG + tig0021037:78256-78275 None:intergenic 40.0%
TCGTAAGTGCAATGACTATG+AGG - tig0021037:78267-78286 Msa1388230:CDS 40.0%
CGTAAGTGCAATGACTATGA+GGG - tig0021037:78268-78287 Msa1388230:CDS 40.0%
GTAAGTGCAATGACTATGAG+GGG - tig0021037:78269-78288 Msa1388230:CDS 40.0%
GCTGTTCGTAATAGAGAACT+TGG + tig0021037:78838-78857 None:intergenic 40.0%
! GAAGGTGAAGCATTTGCAAT+AGG + tig0021037:79094-79113 None:intergenic 40.0%
TCAGCAGAATGAGAATGAGA+AGG + tig0021037:79112-79131 None:intergenic 40.0%
!! AATGATTTTCGAGGTAGTGG+AGG + tig0021037:79139-79158 None:intergenic 40.0%
ATACCATCCAAACGTGGAAA+AGG + tig0021037:79199-79218 None:intergenic 40.0%
GTAACAATACCATCCAAACG+TGG + tig0021037:79205-79224 None:intergenic 40.0%
AACCATATTGTAAAGGGTGG+TGG - tig0021037:79375-79394 Msa1388230:five_prime_UTR 40.0%
AGTGTACCGTATGCTTAGAT+AGG + tig0021037:79530-79549 None:intergenic 40.0%
TCTTTCTGTACTTGCCATGG+TGG - tig0021037:75525-75544 Msa1388230:CDS 45.0%
GTTGAACTCTCTGATGTTCC+TGG - tig0021037:76247-76266 Msa1388230:intron 45.0%
CATCGCTATGCTTAGATGTG+TGG - tig0021037:76339-76358 Msa1388230:intron 45.0%
! AGAGACCAGTTGTTGATTGG+TGG - tig0021037:76638-76657 Msa1388230:intron 45.0%
! GAGACCAGTTGTTGATTGGT+GGG - tig0021037:76639-76658 Msa1388230:intron 45.0%
GCAACAGACACAACTTGTGT+CGG + tig0021037:77027-77046 None:intergenic 45.0%
GACACAACTTGTGTAAGTGC+GGG + tig0021037:77049-77068 None:intergenic 45.0%
AGTTGCTTGCAGGCTTGATT+TGG - tig0021037:77505-77524 Msa1388230:intron 45.0%
TCCCCGGTAAATGAATACGA+AGG + tig0021037:77584-77603 None:intergenic 45.0%
CACGTTATTCGATGTGGACA+CGG - tig0021037:77604-77623 Msa1388230:intron 45.0%
!! TGAACGGGTTGGAAAGCTAA+TGG - tig0021037:77724-77743 Msa1388230:intron 45.0%
TTGCATGACTGCATGCATAG+AGG + tig0021037:77975-77994 None:intergenic 45.0%
GCAATGACTATGAGGGGATT+CGG - tig0021037:78275-78294 Msa1388230:CDS 45.0%
TGTTATGTTGTTCCCACGAG+AGG + tig0021037:78304-78323 None:intergenic 45.0%
AGAGAACTTGGACAACGGTT+TGG + tig0021037:78826-78845 None:intergenic 45.0%
! GCTTTCTCCTTTTCCACGTT+TGG - tig0021037:79189-79208 Msa1388230:CDS 45.0%
! TCTCCTTTTCCACGTTTGGA+TGG - tig0021037:79193-79212 Msa1388230:CDS 45.0%
CGACGACACTCTATTTCCTA+AGG - tig0021037:79256-79275 Msa1388230:five_prime_UTR 45.0%
GGGAACCATATTGTAAAGGG+TGG - tig0021037:79372-79391 Msa1388230:five_prime_UTR 45.0%
CACCACCACCCTTTACAATA+TGG + tig0021037:79380-79399 None:intergenic 45.0%
ATGAGAGTAGTACACCAGCA+AGG + tig0021037:79442-79461 None:intergenic 45.0%
!!! TTTAAATAGATTTTTTTAAA+AGG + tig0021037:77917-77936 None:intergenic 5.0%
!!! TTTTAAAAAAATCTATTTAA+AGG - tig0021037:77916-77935 Msa1388230:intron 5.0%
GAGTTCAACGAAGGACACGT+CGG + tig0021037:76236-76255 None:intergenic 50.0%
GAACTCTCTGATGTTCCTGG+TGG - tig0021037:76250-76269 Msa1388230:intron 50.0%
TCAAATGCTTCTGCACCACC+AGG + tig0021037:76268-76287 None:intergenic 50.0%
!! AGAAGTGATAGCGGCTCTGA+CGG - tig0021037:76586-76605 Msa1388230:intron 50.0%
TCAGCCCACCAATCAACAAC+TGG + tig0021037:76646-76665 None:intergenic 50.0%
CACATCGAATAACGTGTCCC+CGG + tig0021037:77600-77619 None:intergenic 50.0%
TCGATGTGGACACGGTTCAA+AGG - tig0021037:77612-77631 Msa1388230:intron 50.0%
GCGGTCCGATCTTCATTCAA+TGG + tig0021037:78169-78188 None:intergenic 50.0%
CACATGCAGCTCAAAACGAC+CGG - tig0021037:78792-78811 Msa1388230:intron 50.0%
!! CAACGGTTTGGACTGGTAAC+CGG + tig0021037:78814-78833 None:intergenic 50.0%
! ACTTGGACAACGGTTTGGAC+TGG + tig0021037:78821-78840 None:intergenic 50.0%
CGACTTCGCGATGCAATGAA+TGG - tig0021037:78860-78879 Msa1388230:intron 50.0%
!! GAGTGTCGTCGATTCTTGCT+TGG + tig0021037:79247-79266 None:intergenic 50.0%
GGATTGCATTGCAACCTTGC+TGG - tig0021037:79425-79444 Msa1388230:five_prime_UTR 50.0%
!! GTACTTGCCATGGTGGATCG+TGG - tig0021037:75532-75551 Msa1388230:CDS 55.0%
GTCTAGACCACGATCCACCA+TGG + tig0021037:75542-75561 None:intergenic 55.0%
CGGGGACACGTTATTCGATG+TGG - tig0021037:77598-77617 Msa1388230:intron 55.0%
GGGATTCGGATACCTCTCGT+GGG - tig0021037:78289-78308 Msa1388230:CDS 55.0%
GGGGATTCGGATACCTCTCG+TGG - tig0021037:78288-78307 Msa1388230:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0021037 gene 75200 79561 75200 ID=Msa1388230;Name=Msa1388230
tig0021037 mRNA 75200 79561 75200 ID=Msa1388230-mRNA-1;Parent=Msa1388230;Name=Msa1388230-mRNA-1;_AED=0.02;_eAED=0.02;_QI=341|1|1|1|1|1|5|285|630
tig0021037 exon 75200 76051 75200 ID=Msa1388230-mRNA-1:exon:11041;Parent=Msa1388230-mRNA-1
tig0021037 exon 76881 77417 76881 ID=Msa1388230-mRNA-1:exon:11040;Parent=Msa1388230-mRNA-1
tig0021037 exon 77969 78586 77969 ID=Msa1388230-mRNA-1:exon:11039;Parent=Msa1388230-mRNA-1
tig0021037 exon 78919 78988 78919 ID=Msa1388230-mRNA-1:exon:11038;Parent=Msa1388230-mRNA-1
tig0021037 exon 79120 79561 79120 ID=Msa1388230-mRNA-1:exon:11037;Parent=Msa1388230-mRNA-1
tig0021037 five_prime_UTR 79221 79561 79221 ID=Msa1388230-mRNA-1:five_prime_utr;Parent=Msa1388230-mRNA-1
tig0021037 CDS 79120 79220 79120 ID=Msa1388230-mRNA-1:cds;Parent=Msa1388230-mRNA-1
tig0021037 CDS 78919 78988 78919 ID=Msa1388230-mRNA-1:cds;Parent=Msa1388230-mRNA-1
tig0021037 CDS 77969 78586 77969 ID=Msa1388230-mRNA-1:cds;Parent=Msa1388230-mRNA-1
tig0021037 CDS 76881 77417 76881 ID=Msa1388230-mRNA-1:cds;Parent=Msa1388230-mRNA-1
tig0021037 CDS 75485 76051 75485 ID=Msa1388230-mRNA-1:cds;Parent=Msa1388230-mRNA-1
tig0021037 three_prime_UTR 75200 75484 75200 ID=Msa1388230-mRNA-1:three_prime_utr;Parent=Msa1388230-mRNA-1
Gene Sequence

>Msa1388230

ATGGTGGATCGTGGTCTAGACAAAACTACAACATCTGCTAGTGACTTGTCTCTGAAGAAGCAAGAGCTTCTATCTAGTGCTATGAAAAGAACAAGTGACTGGATTTTTTCTCAGGAGATTCCAAGTGATGTCAATGTCCAAATTGGAGAATCTTCCTTTACTTTACATAAGTATCCATTAGTCTCCAAGTGTGGATACATTCGGAAACTCGTCTCAGAATCTAATGACTCCGACGTGTCCTTCGTTGAACTCTCTGATGTTCCTGGTGGTGCAGAAGCATTTGAACTAGCAGCAAAATTCTGCTACGGTATAAATTTCGAGATAAATGTGGAAAACATCGCTATGCTTAGATGTGTGGCCGAATATCTTGAGATGACAGAAGATTATTCAGTTGGGAACTTGGTGGCAAGAACTGATGCATATTTGAATGATGTAGCACTAAAAACAATTGCAGGTTCTGTTTCAATCTTACATATTTCAGAAAGCCTTCTTCCAATAGCAGAGAAAACAAAATTGGTGAGCAAATGCATAGATGCAATTGCTTATATTGCTTGCAAGGAAAGTCAATTGTGTTCATCTGTAAGAAGTGATAGCGGCTCTGACGGAGTGATGTCATCTTCTGTGTCATCTCAACAGAGACCAGTTGTTGATTGGTGGGCTGAAGATCTAACTGTTCTTAGAATCGACCTTTTTCAAAGAGTTCTTATAGCAATGATGGCTAGAGGGTTTAAACAGTTTGCTATTGGTCCTATTCTAATGCTCTATGCACAGAAATCTCTACGAGGTTTGGATATATTTGGAAAGGGAAGGAAGAAGATTGAACCAAGACAAGAGCATGAAAAAAGGGTTATTTTAGAGACAATAGTGAGCCTATTACCAAAAGAGAAGAATGCAATGTCAGTAAGCTTTCTATCGATGCTGCTTCGTGCAGCAATATATCTTGATACAACAGTTGCTTGCAGGCTTGATTTGGAGAAAAGAATGGCAATGCAATTAGGACAAGCTGTCTTAGATGATCTTCTCATTCCTTCGTATTCATTTACCGGGGACACGTTATTCGATGTGGACACGGTTCAAAGGATCATGAATAATTACCAAGAATCTCAAATTGGAAACCACTTTGTCTATAATGGTGATGATGAATATTTTTCTCCACCACAAAGTGATATTGAACGGGTTGGAAAGCTAATGGAAAACTATCTTGCTGAAATAGCCACTGACAGAAATTTATCAGTTGCAAAGTTCATCAGTCTAGCTGAATTGATTCCTGAACAATCAAAACCTACAGAAGATGGGATGTATAGAGCCTTAGACATCTATTTAAAGGCTCATCCCAATTTAAGTGACATGGAGAGAAAGAAAGTTTGCAGCATAATGGACTGTCAAAGACTATCACGAGAAGCATGTGCACATGCAGCTCAAAACGACCGGTTACCAGTCCAAACCGTTGTCCAAGTTCTCTATTACGAACAGCAACGACTTCGCGATGCAATGAATGGAAGTGAAACTGCAGAATCACCTTCTATTCACACCAAACTAAATGTATATTCAGGTGATATTCAAGTTTCAAATGAACTCACAAGCTTACGAAGAGAAAATGAAGACCTCAAATTAGAGGTAGTGAAGTTGAAAATGAAACTAAAAGAGATCGAGATTTCCACGCTTAAATCAACTGTTAAATCAACGGTAAAATCAACGGTTAATAGTCCTATTGCAAATGCTTCACCTTCTCATTCTCATTCTGCTGATAAGCCTCCACTACCTCGAAAATCATTTATAAGTTCGGTTTCAAAGAAACTTGGGAAGCTTTCTCCTTTTCCACGTTTGGATGGTATTGTTACTGCTAAAGGTAGGACAAAACCAAGCAAGAATCGACGACACTCTATTTCCTAA
Protein sequence

>Msa1388230

MVDRGLDKTTTSASDLSLKKQELLSSAMKRTSDWIFSQEIPSDVNVQIGESSFTLHKYPLVSKCGYIRKLVSESNDSDVSFVELSDVPGGAEAFELAAKFCYGINFEINVENIAMLRCVAEYLEMTEDYSVGNLVARTDAYLNDVALKTIAGSVSILHISESLLPIAEKTKLVSKCIDAIAYIACKESQLCSSVRSDSGSDGVMSSSVSSQQRPVVDWWAEDLTVLRIDLFQRVLIAMMARGFKQFAIGPILMLYAQKSLRGLDIFGKGRKKIEPRQEHEKRVILETIVSLLPKEKNAMSVSFLSMLLRAAIYLDTTVACRLDLEKRMAMQLGQAVLDDLLIPSYSFTGDTLFDVDTVQRIMNNYQESQIGNHFVYNGDDEYFSPPQSDIERVGKLMENYLAEIATDRNLSVAKFISLAELIPEQSKPTEDGMYRALDIYLKAHPNLSDMERKKVCSIMDCQRLSREACAHAAQNDRLPVQTVVQVLYYEQQRLRDAMNGSETAESPSIHTKLNVYSGDIQVSNELTSLRRENEDLKLEVVKLKMKLKEIEISTLKSTVKSTVKSTVNSPIANASPSHSHSADKPPLPRKSFISSVSKKLGKLSPFPRLDGIVTAKGRTKPSKNRRHSIS*