AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0714020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0714020 MtrunA17_Chr5g0401451 98.730 630 8 0 1 630 1 630 0.0 1286
Msa0714020 MtrunA17_Chr4g0053841 70.279 609 161 9 28 630 1 595 0.0 854
Msa0714020 MtrunA17_Chr4g0010091 41.694 614 316 8 6 606 1 585 1.31e-166 489
Msa0714020 MtrunA17_Chr1g0195911 41.784 639 300 17 25 630 22 621 9.44e-158 467
Msa0714020 MtrunA17_Chr7g0268781 40.469 640 315 14 25 630 22 629 1.50e-153 456
Msa0714020 MtrunA17_Chr5g0432861 39.094 596 297 7 24 606 9 551 6.82e-146 435
Msa0714020 MtrunA17_Chr3g0108331 40.681 617 300 12 24 630 8 568 4.09e-145 432
Msa0714020 MtrunA17_Chr7g0245201 41.531 614 311 17 35 630 57 640 9.09e-143 429
Msa0714020 MtrunA17_Chr4g0050021 38.356 584 289 8 29 551 33 606 5.59e-132 402
Msa0714020 MtrunA17_Chr2g0279481 36.780 590 298 7 28 551 32 612 3.45e-126 388
Msa0714020 MtrunA17_Chr2g0303541 38.278 546 305 11 34 551 20 561 5.96e-126 386
Msa0714020 MtrunA17_Chr8g0382231 36.269 579 301 11 10 551 19 566 6.43e-121 372
Msa0714020 MtrunA17_Chr2g0285511 35.636 550 299 10 34 536 20 561 1.14e-114 355
Msa0714020 MtrunA17_Chr2g0320691 35.043 585 341 13 24 576 7 584 4.41e-114 355
Msa0714020 MtrunA17_Chr8g0374011 34.522 617 326 14 24 567 1 612 2.94e-113 353
Msa0714020 MtrunA17_Chr1g0207461 38.205 479 259 11 37 493 25 488 2.50e-103 327
Msa0714020 MtrunA17_Chr1g0181181 35.306 490 269 7 34 499 20 485 2.96e-103 324
Msa0714020 MtrunA17_Chr5g0401341 38.936 470 264 9 37 493 24 483 2.77e-102 324
Msa0714020 MtrunA17_Chr1g0150191 38.136 472 268 11 36 491 23 486 5.88e-101 320
Msa0714020 MtrunA17_Chr5g0412591 37.972 503 232 10 36 493 22 489 2.94e-100 318
Msa0714020 MtrunA17_Chr1g0199631 36.674 469 260 7 58 500 8 465 5.95e-98 311
Msa0714020 MtrunA17_Chr4g0076521 35.408 466 281 7 36 493 22 475 2.50e-93 300
Msa0714020 MtrunA17_Chr8g0367891 35.433 508 270 12 43 519 29 509 4.93e-88 285
Msa0714020 MtrunA17_Chr2g0290741 35.000 460 275 7 39 493 25 465 2.95e-85 278
Msa0714020 MtrunA17_Chr1g0191711 30.996 542 323 12 26 554 44 547 1.37e-80 265
Msa0714020 MtrunA17_Chr1g0206621 69.343 137 40 1 47 181 75 211 3.45e-61 203
Msa0714020 MtrunA17_Chr4g0003891 39.362 282 152 4 219 494 46 314 1.75e-58 203
Msa0714020 MtrunA17_Chr2g0332281 36.119 335 187 6 219 544 24 340 2.28e-55 194
Msa0714020 MtrunA17_Chr4g0023801 27.405 551 331 19 43 558 11 527 3.38e-49 180
Msa0714020 MtrunA17_Chr4g0037661 25.185 540 345 15 43 551 6 517 4.32e-41 157
Msa0714020 MtrunA17_Chr1g0163311 25.801 562 329 20 43 562 6 521 2.22e-38 149
Msa0714020 MtrunA17_Chr2g0280541 25.799 407 250 13 107 495 2 374 3.02e-26 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0714020 AT5G67385.1 67.851 619 180 8 16 630 1 604 0.0 826
Msa0714020 AT5G67385.4 67.770 574 166 8 61 630 1 559 0.0 759
Msa0714020 AT5G67385.5 67.770 574 166 8 61 630 1 559 0.0 759
Msa0714020 AT5G67385.2 67.770 574 166 8 61 630 1 559 0.0 759
Msa0714020 AT5G67385.3 67.770 574 166 8 61 630 1 559 0.0 759
Msa0714020 AT3G49970.1 54.795 584 185 15 52 630 6 515 0.0 583
Msa0714020 AT3G49970.2 54.795 584 185 15 52 630 6 515 0.0 583
Msa0714020 AT5G48800.1 45.017 602 275 12 20 606 22 582 5.93e-164 483
Msa0714020 AT2G30520.1 40.514 622 313 10 24 630 14 593 3.77e-151 449
Msa0714020 AT1G03010.1 40.678 649 308 15 24 630 21 634 6.92e-150 447
Msa0714020 AT1G03010.2 40.852 634 299 14 39 630 8 607 1.29e-147 441
Msa0714020 AT1G03010.3 40.852 634 299 14 39 630 34 633 1.66e-147 441
Msa0714020 AT2G47860.1 42.986 556 270 12 25 551 23 560 1.13e-143 431
Msa0714020 AT2G47860.3 41.278 579 270 13 25 551 23 583 2.20e-138 419
Msa0714020 AT3G08570.1 42.589 533 278 13 35 551 30 550 3.88e-134 406
Msa0714020 AT2G30520.2 39.761 586 296 10 60 630 2 545 1.19e-132 400
Msa0714020 AT2G30520.3 39.761 586 296 10 60 630 2 545 1.19e-132 400
Msa0714020 AT1G30440.1 35.993 589 323 12 25 566 10 591 3.40e-120 372
Msa0714020 AT3G08660.1 37.914 604 298 14 35 626 30 568 8.71e-119 365
Msa0714020 AT5G13600.1 38.855 489 269 8 34 500 20 500 3.59e-115 357
Msa0714020 AT5G64330.2 35.823 589 274 9 30 516 42 628 3.02e-113 354
Msa0714020 AT5G64330.1 33.888 661 311 11 30 569 42 697 6.56e-113 355
Msa0714020 AT5G03250.1 35.522 594 330 16 25 577 10 591 1.75e-109 342
Msa0714020 AT3G08660.3 37.587 572 281 13 67 626 1 508 2.33e-109 339
Msa0714020 AT2G47860.4 43.379 438 211 10 25 442 23 443 5.51e-109 335
Msa0714020 AT5G66560.1 36.229 541 272 11 34 508 13 546 1.68e-106 336
Msa0714020 AT3G44820.1 33.333 585 339 10 24 563 7 585 1.79e-105 333
Msa0714020 AT2G47860.6 41.215 461 211 11 25 442 23 466 1.11e-103 323
Msa0714020 AT5G64330.3 35.159 566 263 9 53 516 6 569 2.17e-103 326
Msa0714020 AT2G47860.5 40.789 456 227 10 125 551 1 442 1.63e-101 319
Msa0714020 AT2G47860.2 40.789 456 227 10 125 551 1 442 1.63e-101 319
Msa0714020 AT5G66560.3 36.711 523 258 11 52 508 2 517 2.58e-101 322
Msa0714020 AT5G66560.4 40.312 449 239 10 82 508 66 507 6.01e-101 321
Msa0714020 AT2G14820.2 36.810 489 276 10 39 507 26 501 1.31e-100 320
Msa0714020 AT2G14820.1 36.810 489 276 10 39 507 26 501 1.31e-100 320
Msa0714020 AT1G67900.5 38.095 525 251 11 37 508 23 526 3.33e-99 316
Msa0714020 AT1G67900.1 38.095 525 251 11 37 508 23 526 3.33e-99 316
Msa0714020 AT1G67900.3 38.095 525 251 11 37 508 23 526 3.33e-99 316
Msa0714020 AT1G67900.6 38.095 525 251 11 37 508 23 526 3.33e-99 316
Msa0714020 AT1G67900.2 38.095 525 251 11 37 508 23 526 3.33e-99 316
Msa0714020 AT1G67900.4 38.095 525 251 11 37 508 23 526 3.33e-99 316
Msa0714020 AT3G50840.1 33.962 530 318 7 29 551 5 509 3.81e-98 311
Msa0714020 AT5G10250.2 33.391 578 313 13 23 562 33 576 5.02e-98 313
Msa0714020 AT5G10250.1 33.275 568 312 13 23 562 33 561 7.01e-96 307
Msa0714020 AT4G31820.1 35.477 482 275 11 39 508 26 483 3.33e-91 293
Msa0714020 AT4G37590.1 35.174 489 270 10 37 509 24 481 4.27e-88 286
Msa0714020 AT3G50840.2 34.137 498 296 7 61 551 1 473 1.11e-87 283
Msa0714020 AT3G50840.3 34.137 498 296 7 61 551 1 473 1.11e-87 283
Msa0714020 AT5G67440.3 35.714 462 283 9 37 493 24 476 2.58e-87 283
Msa0714020 AT5G67440.1 35.714 462 283 9 37 493 24 476 2.58e-87 283
Msa0714020 AT2G23050.1 33.824 476 269 10 37 506 24 459 3.09e-84 273
Msa0714020 AT5G47800.3 32.238 487 295 10 35 508 21 485 4.33e-83 272
Msa0714020 AT5G47800.1 32.238 487 295 10 35 508 21 485 4.33e-83 272
Msa0714020 AT5G47800.4 32.238 487 295 10 35 508 21 485 4.33e-83 272
Msa0714020 AT3G08660.2 39.474 418 219 10 35 446 30 419 1.35e-82 266
Msa0714020 AT5G67440.2 35.360 444 273 9 55 493 5 439 1.32e-81 267
Msa0714020 AT5G47800.2 32.238 487 295 10 35 508 134 598 1.83e-81 270
Msa0714020 AT4G31820.2 34.802 454 260 11 67 508 1 430 5.17e-80 263
Msa0714020 AT3G26490.1 32.685 514 287 12 37 504 23 523 1.07e-79 264
Msa0714020 AT3G26490.3 32.685 514 287 12 37 504 23 523 1.07e-79 264
Msa0714020 AT3G26490.4 32.685 514 287 12 37 504 23 523 1.07e-79 264
Msa0714020 AT5G03250.2 34.151 489 276 13 125 577 1 479 5.95e-76 251
Msa0714020 AT5G66560.2 33.122 474 245 10 34 442 13 479 1.81e-75 249
Msa0714020 AT3G26490.2 31.264 451 256 11 37 446 23 460 5.83e-60 209
Msa0714020 AT1G52770.1 36.624 314 165 5 193 494 13 304 2.21e-56 197
Msa0714020 AT2G23050.2 32.898 383 216 9 125 506 1 343 7.48e-56 194
Msa0714020 AT5G48130.1 28.390 472 287 12 34 491 33 467 4.33e-55 198
Msa0714020 AT3G15570.1 35.789 285 152 4 228 494 38 309 5.92e-51 183
Msa0714020 AT3G19850.1 27.669 459 276 10 65 491 29 463 1.27e-47 176
Msa0714020 AT1G50280.1 28.753 473 293 12 42 487 5 460 3.68e-46 171
Msa0714020 AT1G50280.2 29.977 427 261 10 82 487 18 427 1.88e-45 169
Msa0714020 AT3G22104.1 27.193 456 272 10 42 478 6 420 5.20e-40 154
Msa0714020 AT3G03510.1 27.696 408 239 9 94 496 8 364 4.13e-38 147
Msa0714020 AT5G17580.2 25.745 470 307 10 42 498 22 462 1.19e-37 147
Msa0714020 AT5G17580.1 25.745 470 307 10 42 498 7 447 1.68e-37 147
Msa0714020 AT3G22104.4 25.587 383 228 7 112 478 4 345 9.44e-28 117
Msa0714020 AT3G22104.2 25.587 383 228 7 112 478 4 345 9.44e-28 117
Msa0714020 AT3G22104.3 25.587 383 228 7 112 478 4 345 9.44e-28 117
Msa0714020 AT3G49900.2 31.795 195 112 7 30 213 55 239 1.30e-13 74.3
Msa0714020 AT3G49900.1 31.250 192 117 6 30 213 55 239 1.47e-13 73.9

Find 135 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTCCATGTCACTTAAAT+TGG 0.099646 5_1:+6586885 None:intergenic
AGCCTTAGACATCTATTTAA+AGG 0.193457 5_1:-6587741 Msa0714020:intron
TCTTTCGAGCAGGATATATT+TGG 0.214701 5_1:-6588268 Msa0714020:intron
TAAAGGAAGATTCTCCAATT+TGG 0.228988 5_1:+6589791 None:intergenic
GACATTGCATTCTTCTCTTT+TGG 0.249323 5_1:+6588169 None:intergenic
GGAAACCACTTTGTCTATAA+TGG 0.269644 5_1:-6587935 Msa0714020:CDS
CACCACCACCCTTTACAATA+TGG 0.282070 5_1:+6586225 None:intergenic
GGTTTCCAATTTGAGATTCT+TGG 0.291899 5_1:+6587951 None:intergenic
CTAGATAAGAAGACCTTGTT+TGG 0.292025 5_1:-6590357 Msa0714020:five_prime_UTR
ATAACTTAAGAAAATGTTAA+AGG 0.301852 5_1:-6590182 Msa0714020:five_prime_UTR
ATTCCTTCGTATTCATTTAC+CGG 0.307235 5_1:-6588022 Msa0714020:CDS
TACCTTTAAATAGATGTCTA+AGG 0.307550 5_1:+6587739 None:intergenic
AGTTGCTTGCAGGCTTGATT+TGG 0.310330 5_1:-6588095 Msa0714020:CDS
TTTAGTTTGGTGTGAATAGA+AGG 0.321571 5_1:+6586700 None:intergenic
CTATAAGAACTCTTTGAAAA+AGG 0.323564 5_1:+6588910 None:intergenic
AATAGCAGAGAAAACAAAAT+TGG 0.326409 5_1:-6589102 Msa0714020:CDS
TTGATACAACAGTTGCTTGC+AGG 0.331579 5_1:-6588105 Msa0714020:CDS
GTTGAACTCTCTGATGTTCC+TGG 0.333752 5_1:-6589353 Msa0714020:CDS
ACCTGAATATACATTTAGTT+TGG 0.337497 5_1:+6586687 None:intergenic
AAAAGAATGGCAATGCAATT+AGG 0.350908 5_1:-6588070 Msa0714020:CDS
TCGAAAATCATTTATAAGTT+CGG 0.354375 5_1:-6586455 Msa0714020:CDS
AATTACCAAGAATCTCAAAT+TGG 0.354888 5_1:-6587956 Msa0714020:CDS
AGTGATGTCAATGTCCAAAT+TGG 0.359152 5_1:-6589805 Msa0714020:CDS
CTCTCCATGTCACTTAAATT+GGG 0.374237 5_1:+6586886 None:intergenic
TTCTGTCATCTCAAGATATT+CGG 0.376339 5_1:+6589238 None:intergenic
TCACAAGGTCAAATATTTGT+TGG 0.378136 5_1:-6590329 Msa0714020:five_prime_UTR
CTAGAAGAAAGGTAATTCTA+TGG 0.387879 5_1:+6590376 None:intergenic
ATAGAAATTTATTTGATTTG+AGG 0.387896 5_1:-6590295 Msa0714020:five_prime_UTR
ATAAAATATAATTCTTGAGT+TGG 0.390624 5_1:-6586198 Msa0714020:three_prime_UTR
AAGGAATCTTCAAATATAAA+AGG 0.394765 5_1:-6590163 Msa0714020:five_prime_UTR
AAAGAAAGTTTGCAGCATAA+TGG 0.400198 5_1:-6586863 Msa0714020:CDS
AGATTATTCAGTTGGGAACT+TGG 0.401252 5_1:-6589216 Msa0714020:CDS
GGATTGCATTGCAACCTTGC+TGG 0.406623 5_1:-6586177 Msa0714020:three_prime_UTR
TATCCACACTTGGAGACTAA+TGG 0.409668 5_1:+6589422 None:intergenic
AGAGAACTTGGACAACGGTT+TGG 0.419568 5_1:+6586779 None:intergenic
AACAGAGACCAGTTGTTGAT+TGG 0.421313 5_1:-6588965 Msa0714020:CDS
AGTTCGGTTTCAAAGAAACT+TGG 0.421844 5_1:-6586439 Msa0714020:CDS
AGTGGGAACCATATTGTAAA+GGG 0.423439 5_1:-6586233 Msa0714020:three_prime_UTR
GGGTTTAAACAGTTTGCTAT+TGG 0.424370 5_1:-6588873 Msa0714020:CDS
AAGAGTTCTTATAGCAATGA+TGG 0.430446 5_1:-6588901 Msa0714020:CDS
AAATGAAGACCTCAAATTAG+AGG 0.432193 5_1:-6586623 Msa0714020:CDS
CGACTTCGCGATGCAATGAA+TGG 0.432759 5_1:-6586742 Msa0714020:CDS
ATCTTCCTTTACTTTACATA+AGG 0.434540 5_1:-6589779 Msa0714020:intron
ATGACAGAAGATTATTCAGT+TGG 0.439432 5_1:-6589224 Msa0714020:CDS
CAATCAAAACCTACAGAAGA+TGG 0.439804 5_1:-6587773 Msa0714020:CDS
TGAACGGGTTGGAAAGCTAA+TGG 0.443135 5_1:-6587876 Msa0714020:CDS
GAAGGTGAAGCATTTGCAAT+AGG 0.443580 5_1:+6586511 None:intergenic
GATGGTATTGTTACTGCTAA+AGG 0.444847 5_1:-6586391 Msa0714020:CDS
TAGATAAGAAGACCTTGTTT+GGG 0.445988 5_1:-6590356 Msa0714020:five_prime_UTR
CAACGGTTTGGACTGGTAAC+CGG 0.447250 5_1:+6586791 None:intergenic
ACTTCACTACCTCTAATTTG+AGG 0.447292 5_1:+6586614 None:intergenic
ACAGAAATCTCTACGAGGTT+TGG 0.458956 5_1:-6588829 Msa0714020:intron
ATTTGGACATTGACATCACT+TGG 0.463936 5_1:+6589808 None:intergenic
AATTGCTTATATTGCTTGCA+AGG 0.465206 5_1:-6589060 Msa0714020:CDS
TCAGCCCACCAATCAACAAC+TGG 0.478880 5_1:+6588957 None:intergenic
CACGTTATTCGATGTGGACA+CGG 0.493140 5_1:-6587996 Msa0714020:CDS
TTCCTTCGTATTCATTTACC+GGG 0.494561 5_1:-6588021 Msa0714020:CDS
AAGTGGGAACCATATTGTAA+AGG 0.496550 5_1:-6586234 Msa0714020:three_prime_UTR
CCTTGTGAATTTCCCAAACA+AGG 0.497170 5_1:+6590344 None:intergenic
CTATACATCCCATCTTCTGT+AGG 0.497214 5_1:+6587764 None:intergenic
ATACCATCCAAACGTGGAAA+AGG 0.497451 5_1:+6586406 None:intergenic
ACCCGTTCAATATCACTTTG+TGG 0.498785 5_1:+6587890 None:intergenic
ACCACAAAGTGATATTGAAC+GGG 0.498903 5_1:-6587891 Msa0714020:CDS
TGTGCATAGAGCATTAGAAT+AGG 0.499783 5_1:+6588849 None:intergenic
AAGGTCATGAAAATAAATAC+AGG 0.501104 5_1:-6586327 Msa0714020:three_prime_UTR
ATGTCATCACGATGTTAAAG+TGG 0.506615 5_1:-6586251 Msa0714020:three_prime_UTR
TCTCCAAGTGTGGATACATT+CGG 0.506959 5_1:-6589415 Msa0714020:CDS
CGAGCAGGATATATTTGGAA+AGG 0.507201 5_1:-6588263 Msa0714020:intron
CACTACCTTATGTAAAGTAA+AGG 0.508799 5_1:+6589774 None:intergenic
GCTTGATTTGGAGAAAAGAA+TGG 0.510077 5_1:-6588083 Msa0714020:CDS
ACCAAACTAAATGTATATTC+AGG 0.522428 5_1:-6586688 Msa0714020:CDS
GAGTGTCGTCGATTCTTGCT+TGG 0.523000 5_1:+6586358 None:intergenic
AGAGACCAGTTGTTGATTGG+TGG 0.523518 5_1:-6588962 Msa0714020:CDS
GAGCAGGATATATTTGGAAA+GGG 0.526552 5_1:-6588262 Msa0714020:intron
AGTGTACCGTATGCTTAGAT+AGG 0.529667 5_1:+6586076 None:intergenic
TGACAGAAGATTATTCAGTT+GGG 0.532521 5_1:-6589223 Msa0714020:CDS
GGTCTTCTTATCTAGAAGAA+AGG 0.533076 5_1:+6590365 None:intergenic
AAATCTTTCTGTACTTGCCA+TGG 0.535061 5_1:-6590078 Msa0714020:exon
GCTGTTCGTAATAGAGAACT+TGG 0.535435 5_1:+6586767 None:intergenic
AACTGATAAATTTCTGTCAG+TGG 0.538405 5_1:+6587832 None:intergenic
CACCACAAAGTGATATTGAA+CGG 0.544352 5_1:-6587892 Msa0714020:CDS
ACTTGGACAACGGTTTGGAC+TGG 0.546671 5_1:+6586784 None:intergenic
TTTCTGCTTCATTTATAGTG+AGG 0.547211 5_1:-6590104 Msa0714020:five_prime_UTR
GTAATAGAGAACTTGGACAA+CGG 0.549721 5_1:+6586774 None:intergenic
TTTCCGAATGTATCCACACT+TGG 0.551161 5_1:+6589412 None:intergenic
AGAAGTGATAGCGGCTCTGA+CGG 0.551480 5_1:-6589014 Msa0714020:CDS
GTTCGGTTTCAAAGAAACTT+GGG 0.553017 5_1:-6586438 Msa0714020:CDS
AGGATATATTTGGAAAGGGA+AGG 0.553677 5_1:-6588258 Msa0714020:CDS
TCTTTCTGTACTTGCCATGG+TGG 0.556166 5_1:-6590075 Msa0714020:exon
CCTTGTTTGGGAAATTCACA+AGG 0.561007 5_1:-6590344 Msa0714020:five_prime_UTR
CGACGACACTCTATTTCCTA+AGG 0.562175 5_1:-6586346 Msa0714020:exon
AACCATATTGTAAAGGGTGG+TGG 0.564776 5_1:-6586227 Msa0714020:three_prime_UTR
TTATTCAGTTGGGAACTTGG+TGG 0.567121 5_1:-6589213 Msa0714020:CDS
CATCACCATTATAGACAAAG+TGG 0.567160 5_1:+6587930 None:intergenic
CAAAGTGATATTGAACGGGT+TGG 0.567740 5_1:-6587887 Msa0714020:CDS
GAAGAAAGGTAATTCTATGG+AGG 0.574630 5_1:+6590379 None:intergenic
TATAAATTTCGAGATAAATG+TGG 0.578053 5_1:-6589288 Msa0714020:CDS
TAAATCAACTGTTAAATCAA+CGG 0.578589 5_1:-6586554 Msa0714020:CDS
CACATGCAGCTCAAAACGAC+CGG 0.579581 5_1:-6586810 Msa0714020:CDS
TCGATGTGGACACGGTTCAA+AGG 0.579585 5_1:-6587988 Msa0714020:CDS
TCAGTTATAGTATGTGCTCA+AGG 0.582768 5_1:+6590139 None:intergenic
ATTCTATGGAGGGTAGATGA+AGG 0.584535 5_1:+6590390 None:intergenic
CTTATAGCAATGATGGCTAG+AGG 0.584871 5_1:-6588894 Msa0714020:CDS
CTATGAAAAGAACAAGTGAC+TGG 0.585425 5_1:-6589980 Msa0714020:intron
TTATAGCAATGATGGCTAGA+GGG 0.589083 5_1:-6588893 Msa0714020:CDS
AATCAAAACCTACAGAAGAT+GGG 0.590751 5_1:-6587772 Msa0714020:CDS
CTAGCAGCAAAATTCTGCTA+CGG 0.592687 5_1:-6589311 Msa0714020:CDS
TCAAATGCTTCTGCACCACC+AGG 0.593786 5_1:+6589335 None:intergenic
AAGAAAGGTAATTCTATGGA+GGG 0.598694 5_1:+6590380 None:intergenic
CACATCGAATAACGTGTCCC+CGG 0.613083 5_1:+6588003 None:intergenic
CGGGGACACGTTATTCGATG+TGG 0.614759 5_1:-6588002 Msa0714020:CDS
TCATCCCAATTTAAGTGACA+TGG 0.614869 5_1:-6586890 Msa0714020:CDS
GGTAGATGAAGGAATATGAG+TGG 0.620727 5_1:+6590401 None:intergenic
TAAATCAACGGTAAAATCAA+CGG 0.622532 5_1:-6586542 Msa0714020:CDS
TCAGCAGAATGAGAATGAGA+AGG 0.624756 5_1:+6586493 None:intergenic
CATCGCTATGCTTAGATGTG+TGG 0.625147 5_1:-6589261 Msa0714020:CDS
GAGACCAGTTGTTGATTGGT+GGG 0.625912 5_1:-6588961 Msa0714020:CDS
GTCTAGACCACGATCCACCA+TGG 0.633131 5_1:+6590061 None:intergenic
TGTCATCACGATGTTAAAGT+GGG 0.635930 5_1:-6586250 Msa0714020:three_prime_UTR
TTTAACAGTTGATTTAAGCG+TGG 0.636314 5_1:+6586561 None:intergenic
CGTTCAATATCACTTTGTGG+TGG 0.641780 5_1:+6587893 None:intergenic
TATCCATTAGTCTCCAAGTG+TGG 0.642032 5_1:-6589425 Msa0714020:CDS
GTATTGTTACTGCTAAAGGT+AGG 0.645750 5_1:-6586387 Msa0714020:CDS
GGGAACCATATTGTAAAGGG+TGG 0.650201 5_1:-6586230 Msa0714020:three_prime_UTR
GTACTTGCCATGGTGGATCG+TGG 0.650434 5_1:-6590068 Msa0714020:exon
TATATTGCGAGAAGTACAAG+TGG 0.662671 5_1:-6586141 Msa0714020:three_prime_UTR
AACATCAGAGAGTTCAACGA+AGG 0.692508 5_1:+6589358 None:intergenic
ATGAGAGTAGTACACCAGCA+AGG 0.699607 5_1:+6586163 None:intergenic
GAACTCTCTGATGTTCCTGG+TGG 0.699693 5_1:-6589350 Msa0714020:CDS
GAGTTCAACGAAGGACACGT+CGG 0.700242 5_1:+6589367 None:intergenic
TCATCTGTAAGAAGTGATAG+CGG 0.715394 5_1:-6589023 Msa0714020:CDS
TCCCCGGTAAATGAATACGA+AGG 0.717871 5_1:+6588019 None:intergenic
GTAACAATACCATCCAAACG+TGG 0.725647 5_1:+6586400 None:intergenic
TCCTTCGTATTCATTTACCG+GGG 0.730046 5_1:-6588020 Msa0714020:CDS
TATGCACAGAAATCTCTACG+AGG 0.833179 5_1:-6588834 Msa0714020:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAATTATTAAGAATTA+AGG + chr5_1:6588174-6588193 None:intergenic 10.0%
!!! ATAGAAATTTATTTGATTTG+AGG - chr5_1:6586169-6586188 Msa0714020:three_prime_UTR 15.0%
!! ATAACTTAAGAAAATGTTAA+AGG - chr5_1:6586282-6586301 Msa0714020:three_prime_UTR 15.0%
!! CAAAAAAATCTTTATATTGA+CGG - chr5_1:6586991-6587010 Msa0714020:intron 15.0%
!!! TTTCCTATCATTAAATTTTT+TGG - chr5_1:6589059-6589078 Msa0714020:CDS 15.0%
!!! GTTAAAATTTTACAATTTTC+AGG - chr5_1:6589550-6589569 Msa0714020:intron 15.0%
!! ATAAAATATAATTCTTGAGT+TGG - chr5_1:6590266-6590285 Msa0714020:five_prime_UTR 15.0%
!! AAGGAATCTTCAAATATAAA+AGG - chr5_1:6586301-6586320 Msa0714020:three_prime_UTR 20.0%
!!! TAATTATAGGATTTTTTCTC+AGG - chr5_1:6586628-6586647 Msa0714020:CDS 20.0%
!! TTAATCAATCATAAAAACGT+AGG + chr5_1:6586966-6586985 None:intergenic 20.0%
!! TATAAATTTCGAGATAAATG+TGG - chr5_1:6587176-6587195 Msa0714020:intron 20.0%
!!! TTTCTATCTTTTTTTTGAGA+GGG + chr5_1:6587667-6587686 None:intergenic 20.0%
!!! TTTTCTATCTTTTTTTTGAG+AGG + chr5_1:6587668-6587687 None:intergenic 20.0%
!! TTAAAAATAAATACTCCATC+CGG - chr5_1:6587689-6587708 Msa0714020:intron 20.0%
!! TATAAAAGTCTAATCAATGT+TGG + chr5_1:6588103-6588122 None:intergenic 20.0%
!!! TAGTAGTTTTAAAATGGATT+TGG + chr5_1:6588911-6588930 None:intergenic 20.0%
!!! TCTAAGTAGTAGTTTTAAAA+TGG + chr5_1:6588917-6588936 None:intergenic 20.0%
!! TAGCCAAAAAATTTAATGAT+AGG + chr5_1:6589065-6589084 None:intergenic 20.0%
!! TTAACAAAATCAAAAGATCA+TGG - chr5_1:6589311-6589330 Msa0714020:CDS 20.0%
!! TAAATCAACTGTTAAATCAA+CGG - chr5_1:6589910-6589929 Msa0714020:intron 20.0%
!! TCGAAAATCATTTATAAGTT+CGG - chr5_1:6590009-6590028 Msa0714020:CDS 20.0%
!!! TCATCTATTTTTTTGTTTGT+GGG + chr5_1:6590356-6590375 None:intergenic 20.0%
!!! TTCATCTATTTTTTTGTTTG+TGG + chr5_1:6590357-6590376 None:intergenic 20.0%
!!! AAAAGTTTTAGGGAATAATG+AGG + chr5_1:6586216-6586235 None:intergenic 25.0%
! AATTAAGCAATACAACTCAT+TGG + chr5_1:6586613-6586632 None:intergenic 25.0%
! GAGTTGTATTGCTTAATTAT+AGG - chr5_1:6586615-6586634 Msa0714020:CDS 25.0%
! ATCTTCCTTTACTTTACATA+AGG - chr5_1:6586685-6586704 Msa0714020:CDS 25.0%
! ATCATAAAAACGTAGGTAAA+AGG + chr5_1:6586959-6586978 None:intergenic 25.0%
!!! AATTTTGTTTTCTCTGCTAT+TGG + chr5_1:6587363-6587382 None:intergenic 25.0%
! AATAGCAGAGAAAACAAAAT+TGG - chr5_1:6587362-6587381 Msa0714020:intron 25.0%
!! CTATAAGAACTCTTTGAAAA+AGG + chr5_1:6587557-6587576 None:intergenic 25.0%
!!! TTCTATCTTTTTTTTGAGAG+GGG + chr5_1:6587666-6587685 None:intergenic 25.0%
!! AATTTGCTTATAATAGTGAC+CGG + chr5_1:6587820-6587839 None:intergenic 25.0%
! CATTCAATAAATGATGTATG+TGG - chr5_1:6587746-6587765 Msa0714020:CDS 25.0%
! CATTCAATAAATGATGTATG+TGG + chr5_1:6587746-6587765 None:intergenic 25.0%
!! AATTTGCTTATAATAGTGAC+CGG - chr5_1:6587820-6587839 Msa0714020:CDS 25.0%
! TGTAATTATATGTGTAAGTG+CGG + chr5_1:6587942-6587961 None:intergenic 25.0%
! AATTACCAAGAATCTCAAAT+TGG - chr5_1:6588508-6588527 Msa0714020:intron 25.0%
! TACCTTTAAATAGATGTCTA+AGG + chr5_1:6588728-6588747 None:intergenic 25.0%
! ATCTATTTACATACAGAGAT+AGG + chr5_1:6588972-6588991 None:intergenic 25.0%
! AGCTAAAAATCTTTATCCAA+TGG - chr5_1:6589099-6589118 Msa0714020:CDS 25.0%
!! CAAATAAATGTATTGCTGTT+TGG - chr5_1:6589275-6589294 Msa0714020:CDS 25.0%
! TTTGTATAGTAACACATGTT+TGG + chr5_1:6589412-6589431 None:intergenic 25.0%
! ACAATAATACATGTTTGGAA+TGG + chr5_1:6589448-6589467 None:intergenic 25.0%
! ACACTACAATAATACATGTT+TGG + chr5_1:6589453-6589472 None:intergenic 25.0%
! ACCAAACTAAATGTATATTC+AGG - chr5_1:6589776-6589795 Msa0714020:intron 25.0%
! ACCTGAATATACATTTAGTT+TGG + chr5_1:6589780-6589799 None:intergenic 25.0%
! TAAATCAACGGTAAAATCAA+CGG - chr5_1:6589922-6589941 Msa0714020:intron 25.0%
!! GAACTTATAAATGATTTTCG+AGG + chr5_1:6590010-6590029 None:intergenic 25.0%
!!! GTATTTATTTTCATGACCTT+AGG + chr5_1:6590137-6590156 None:intergenic 25.0%
! AAGGTCATGAAAATAAATAC+AGG - chr5_1:6590137-6590156 Msa0714020:five_prime_UTR 25.0%
AAGAAAGGTAATTCTATGGA+GGG + chr5_1:6586087-6586106 None:intergenic 30.0%
CTAGAAGAAAGGTAATTCTA+TGG + chr5_1:6586091-6586110 None:intergenic 30.0%
! TAGATAAGAAGACCTTGTTT+GGG - chr5_1:6586108-6586127 Msa0714020:three_prime_UTR 30.0%
TCACAAGGTCAAATATTTGT+TGG - chr5_1:6586135-6586154 Msa0714020:three_prime_UTR 30.0%
TTTCTGCTTCATTTATAGTG+AGG - chr5_1:6586360-6586379 Msa0714020:CDS 30.0%
!! TATTGTTTGCTACCTTTTGT+TGG - chr5_1:6586572-6586591 Msa0714020:CDS 30.0%
TAAAGGAAGATTCTCCAATT+TGG + chr5_1:6586676-6586695 None:intergenic 30.0%
CACTACCTTATGTAAAGTAA+AGG + chr5_1:6586693-6586712 None:intergenic 30.0%
TTGAAAGCAAGATGTATATG+CGG - chr5_1:6586885-6586904 Msa0714020:CDS 30.0%
TTCTGTCATCTCAAGATATT+CGG + chr5_1:6587229-6587248 None:intergenic 30.0%
ATGACAGAAGATTATTCAGT+TGG - chr5_1:6587240-6587259 Msa0714020:intron 30.0%
TGACAGAAGATTATTCAGTT+GGG - chr5_1:6587241-6587260 Msa0714020:intron 30.0%
AATTGCTTATATTGCTTGCA+AGG - chr5_1:6587404-6587423 Msa0714020:intron 30.0%
AAGAGTTCTTATAGCAATGA+TGG - chr5_1:6587563-6587582 Msa0714020:intron 30.0%
GTAATTATATGTGTAAGTGC+GGG + chr5_1:6587941-6587960 None:intergenic 30.0%
!!! ATTTTTTCTTTCTTTCGAGC+AGG - chr5_1:6588186-6588205 Msa0714020:CDS 30.0%
! AAGACAAGAGCATGAAAAAA+GGG - chr5_1:6588243-6588262 Msa0714020:CDS 30.0%
!! CATTCTTCTCTTTTGGTAAT+AGG + chr5_1:6588291-6588310 None:intergenic 30.0%
AAAAGAATGGCAATGCAATT+AGG - chr5_1:6588394-6588413 Msa0714020:intron 30.0%
ATTCCTTCGTATTCATTTAC+CGG - chr5_1:6588442-6588461 Msa0714020:intron 30.0%
AACTGATAAATTTCTGTCAG+TGG + chr5_1:6588635-6588654 None:intergenic 30.0%
AATCAAAACCTACAGAAGAT+GGG - chr5_1:6588692-6588711 Msa0714020:intron 30.0%
AGCCTTAGACATCTATTTAA+AGG - chr5_1:6588723-6588742 Msa0714020:intron 30.0%
TAATGATAGGAAAACACAAG+CGG + chr5_1:6589052-6589071 None:intergenic 30.0%
!! AAGCTCTAGAGAATTAGTTT+AGG + chr5_1:6589228-6589247 None:intergenic 30.0%
ACAAAATCAAAAGATCATGG+TGG - chr5_1:6589314-6589333 Msa0714020:CDS 30.0%
!!! TGTGAAGTTTGTGTTGTTTT+TGG - chr5_1:6589348-6589367 Msa0714020:CDS 30.0%
AAAGAAAGTTTGCAGCATAA+TGG - chr5_1:6589601-6589620 Msa0714020:intron 30.0%
!! TTTAGTTTGGTGTGAATAGA+AGG + chr5_1:6589767-6589786 None:intergenic 30.0%
AAATGAAGACCTCAAATTAG+AGG - chr5_1:6589841-6589860 Msa0714020:intron 30.0%
! TTTAACAGTTGATTTAAGCG+TGG + chr5_1:6589906-6589925 None:intergenic 30.0%
!! ATAAATGATTTTCGAGGTAG+TGG + chr5_1:6590004-6590023 None:intergenic 30.0%
! TTTTCTCCTATCTAAGCATA+CGG - chr5_1:6590382-6590401 Msa0714020:five_prime_UTR 30.0%
GAAGAAAGGTAATTCTATGG+AGG + chr5_1:6586088-6586107 None:intergenic 35.0%
GGTCTTCTTATCTAGAAGAA+AGG + chr5_1:6586102-6586121 None:intergenic 35.0%
CTAGATAAGAAGACCTTGTT+TGG - chr5_1:6586107-6586126 Msa0714020:three_prime_UTR 35.0%
! TGCTGAGCTGAAAAGTTTTA+GGG + chr5_1:6586226-6586245 None:intergenic 35.0%
! ATGCTGAGCTGAAAAGTTTT+AGG + chr5_1:6586227-6586246 None:intergenic 35.0%
TCAGTTATAGTATGTGCTCA+AGG + chr5_1:6586328-6586347 None:intergenic 35.0%
AAATCTTTCTGTACTTGCCA+TGG - chr5_1:6586386-6586405 Msa0714020:CDS 35.0%
CTATGAAAAGAACAAGTGAC+TGG - chr5_1:6586484-6586503 Msa0714020:CDS 35.0%
! TGCTACCTTTTGTTGGATTT+AGG - chr5_1:6586579-6586598 Msa0714020:CDS 35.0%
! GCTACCTTTTGTTGGATTTA+GGG - chr5_1:6586580-6586599 Msa0714020:CDS 35.0%
ATTTGGACATTGACATCACT+TGG + chr5_1:6586659-6586678 None:intergenic 35.0%
AGTGATGTCAATGTCCAAAT+TGG - chr5_1:6586659-6586678 Msa0714020:CDS 35.0%
AGATTATTCAGTTGGGAACT+TGG - chr5_1:6587248-6587267 Msa0714020:intron 35.0%
GTAGCACTAAAAACAATTGC+AGG - chr5_1:6587300-6587319 Msa0714020:intron 35.0%
! TTTTCTCTGCTATTGGAAGA+AGG + chr5_1:6587356-6587375 None:intergenic 35.0%
TCATCTGTAAGAAGTGATAG+CGG - chr5_1:6587441-6587460 Msa0714020:intron 35.0%
TTATAGCAATGATGGCTAGA+GGG - chr5_1:6587571-6587590 Msa0714020:intron 35.0%
!! GGGTTTAAACAGTTTGCTAT+TGG - chr5_1:6587591-6587610 Msa0714020:intron 35.0%
! TGTGCATAGAGCATTAGAAT+AGG + chr5_1:6587618-6587637 None:intergenic 35.0%
! CTATAAAAGCATACTCCTTC+CGG + chr5_1:6587842-6587861 None:intergenic 35.0%
TGTTCATATCTGTGCTTCAT+AGG - chr5_1:6587996-6588015 Msa0714020:CDS 35.0%
! CTTCTTTTCTTTCGCAAACA+AGG + chr5_1:6588031-6588050 None:intergenic 35.0%
TCTTTCGAGCAGGATATATT+TGG - chr5_1:6588196-6588215 Msa0714020:CDS 35.0%
GAGCAGGATATATTTGGAAA+GGG - chr5_1:6588202-6588221 Msa0714020:CDS 35.0%
AGGATATATTTGGAAAGGGA+AGG - chr5_1:6588206-6588225 Msa0714020:CDS 35.0%
!!! CTTTTTTCATGCTCTTGTCT+TGG + chr5_1:6588244-6588263 None:intergenic 35.0%
! CAAGACAAGAGCATGAAAAA+AGG - chr5_1:6588242-6588261 Msa0714020:CDS 35.0%
! GACATTGCATTCTTCTCTTT+TGG + chr5_1:6588298-6588317 None:intergenic 35.0%
!! GCTTGATTTGGAGAAAAGAA+TGG - chr5_1:6588381-6588400 Msa0714020:intron 35.0%
TTCCTTCGTATTCATTTACC+GGG - chr5_1:6588443-6588462 Msa0714020:intron 35.0%
! GGTTTCCAATTTGAGATTCT+TGG + chr5_1:6588516-6588535 None:intergenic 35.0%
GGAAACCACTTTGTCTATAA+TGG - chr5_1:6588529-6588548 Msa0714020:intron 35.0%
CATCACCATTATAGACAAAG+TGG + chr5_1:6588537-6588556 None:intergenic 35.0%
CACCACAAAGTGATATTGAA+CGG - chr5_1:6588572-6588591 Msa0714020:intron 35.0%
ACCACAAAGTGATATTGAAC+GGG - chr5_1:6588573-6588592 Msa0714020:intron 35.0%
!!! TCTGTAGGTTTTGATTGTTC+AGG + chr5_1:6588688-6588707 None:intergenic 35.0%
CAATCAAAACCTACAGAAGA+TGG - chr5_1:6588691-6588710 Msa0714020:intron 35.0%
TCATCCCAATTTAAGTGACA+TGG - chr5_1:6589574-6589593 Msa0714020:intron 35.0%
! CTCTCCATGTCACTTAAATT+GGG + chr5_1:6589581-6589600 None:intergenic 35.0%
! TCTCTCCATGTCACTTAAAT+TGG + chr5_1:6589582-6589601 None:intergenic 35.0%
GTAATAGAGAACTTGGACAA+CGG + chr5_1:6589693-6589712 None:intergenic 35.0%
ACTTCACTACCTCTAATTTG+AGG + chr5_1:6589853-6589872 None:intergenic 35.0%
AGTTCGGTTTCAAAGAAACT+TGG - chr5_1:6590025-6590044 Msa0714020:CDS 35.0%
GTTCGGTTTCAAAGAAACTT+GGG - chr5_1:6590026-6590045 Msa0714020:CDS 35.0%
GATGGTATTGTTACTGCTAA+AGG - chr5_1:6590073-6590092 Msa0714020:exon 35.0%
GTATTGTTACTGCTAAAGGT+AGG - chr5_1:6590077-6590096 Msa0714020:exon 35.0%
ATGTCATCACGATGTTAAAG+TGG - chr5_1:6590213-6590232 Msa0714020:five_prime_UTR 35.0%
TGTCATCACGATGTTAAAGT+GGG - chr5_1:6590214-6590233 Msa0714020:five_prime_UTR 35.0%
AAGTGGGAACCATATTGTAA+AGG - chr5_1:6590230-6590249 Msa0714020:five_prime_UTR 35.0%
AGTGGGAACCATATTGTAAA+GGG - chr5_1:6590231-6590250 Msa0714020:five_prime_UTR 35.0%
! TATATTGCGAGAAGTACAAG+TGG - chr5_1:6590323-6590342 Msa0714020:five_prime_UTR 35.0%
ATTCTATGGAGGGTAGATGA+AGG + chr5_1:6586077-6586096 None:intergenic 40.0%
CCTTGTGAATTTCCCAAACA+AGG + chr5_1:6586123-6586142 None:intergenic 40.0%
! CCTTGTTTGGGAAATTCACA+AGG - chr5_1:6586120-6586139 Msa0714020:three_prime_UTR 40.0%
CAGTCCCTAAATCCAACAAA+AGG + chr5_1:6586587-6586606 None:intergenic 40.0%
! TATCCATTAGTCTCCAAGTG+TGG - chr5_1:6587039-6587058 Msa0714020:intron 40.0%
TATCCACACTTGGAGACTAA+TGG + chr5_1:6587045-6587064 None:intergenic 40.0%
TCTCCAAGTGTGGATACATT+CGG - chr5_1:6587049-6587068 Msa0714020:intron 40.0%
TTTCCGAATGTATCCACACT+TGG + chr5_1:6587055-6587074 None:intergenic 40.0%
AACATCAGAGAGTTCAACGA+AGG + chr5_1:6587109-6587128 None:intergenic 40.0%
CTAGCAGCAAAATTCTGCTA+CGG - chr5_1:6587153-6587172 Msa0714020:intron 40.0%
TTATTCAGTTGGGAACTTGG+TGG - chr5_1:6587251-6587270 Msa0714020:intron 40.0%
AACAGAGACCAGTTGTTGAT+TGG - chr5_1:6587499-6587518 Msa0714020:intron 40.0%
CTTATAGCAATGATGGCTAG+AGG - chr5_1:6587570-6587589 Msa0714020:intron 40.0%
TATGCACAGAAATCTCTACG+AGG - chr5_1:6587630-6587649 Msa0714020:intron 40.0%
ACAGAAATCTCTACGAGGTT+TGG - chr5_1:6587635-6587654 Msa0714020:intron 40.0%
TGCTTATAATAGTGACCGGA+TGG + chr5_1:6587824-6587843 None:intergenic 40.0%
TGCTTATAATAGTGACCGGA+AGG - chr5_1:6587824-6587843 Msa0714020:CDS 40.0%
AGACACAACTTGTGTAAGTG+CGG + chr5_1:6587914-6587933 None:intergenic 40.0%
CGAGCAGGATATATTTGGAA+AGG - chr5_1:6588201-6588220 Msa0714020:CDS 40.0%
TTGATACAACAGTTGCTTGC+AGG - chr5_1:6588359-6588378 Msa0714020:intron 40.0%
TCCTTCGTATTCATTTACCG+GGG - chr5_1:6588444-6588463 Msa0714020:intron 40.0%
CGTTCAATATCACTTTGTGG+TGG + chr5_1:6588574-6588593 None:intergenic 40.0%
ACCCGTTCAATATCACTTTG+TGG + chr5_1:6588577-6588596 None:intergenic 40.0%
CAAAGTGATATTGAACGGGT+TGG - chr5_1:6588577-6588596 Msa0714020:intron 40.0%
CTATACATCCCATCTTCTGT+AGG + chr5_1:6588703-6588722 None:intergenic 40.0%
!! CATAGAGGAAGTGTAGCTTT+TGG + chr5_1:6588824-6588843 None:intergenic 40.0%
ATGCAATCACAGATCTCAGT+CGG - chr5_1:6588853-6588872 Msa0714020:CDS 40.0%
GATCTCAGTCGGATGAAAAT+TGG - chr5_1:6588864-6588883 Msa0714020:CDS 40.0%
CTCGACCATTGAATGAAGAT+CGG - chr5_1:6589025-6589044 Msa0714020:CDS 40.0%
CACTTACGAATCTCTACCAT+TGG + chr5_1:6589118-6589137 None:intergenic 40.0%
TCGTAAGTGCAATGACTATG+AGG - chr5_1:6589129-6589148 Msa0714020:CDS 40.0%
CGTAAGTGCAATGACTATGA+GGG - chr5_1:6589130-6589149 Msa0714020:CDS 40.0%
GTAAGTGCAATGACTATGAG+GGG - chr5_1:6589131-6589150 Msa0714020:CDS 40.0%
GCTGTTCGTAATAGAGAACT+TGG + chr5_1:6589700-6589719 None:intergenic 40.0%
! GAAGGTGAAGCATTTGCAAT+AGG + chr5_1:6589956-6589975 None:intergenic 40.0%
TCAGCAGAATGAGAATGAGA+AGG + chr5_1:6589974-6589993 None:intergenic 40.0%
!! AATGATTTTCGAGGTAGTGG+AGG + chr5_1:6590001-6590020 None:intergenic 40.0%
ATACCATCCAAACGTGGAAA+AGG + chr5_1:6590061-6590080 None:intergenic 40.0%
GTAACAATACCATCCAAACG+TGG + chr5_1:6590067-6590086 None:intergenic 40.0%
AACCATATTGTAAAGGGTGG+TGG - chr5_1:6590237-6590256 Msa0714020:five_prime_UTR 40.0%
AGTGTACCGTATGCTTAGAT+AGG + chr5_1:6590391-6590410 None:intergenic 40.0%
TCTTTCTGTACTTGCCATGG+TGG - chr5_1:6586389-6586408 Msa0714020:CDS 45.0%
GTTGAACTCTCTGATGTTCC+TGG - chr5_1:6587111-6587130 Msa0714020:intron 45.0%
CATCGCTATGCTTAGATGTG+TGG - chr5_1:6587203-6587222 Msa0714020:intron 45.0%
! AGAGACCAGTTGTTGATTGG+TGG - chr5_1:6587502-6587521 Msa0714020:intron 45.0%
! GAGACCAGTTGTTGATTGGT+GGG - chr5_1:6587503-6587522 Msa0714020:intron 45.0%
GCAACAGACACAACTTGTGT+CGG + chr5_1:6587891-6587910 None:intergenic 45.0%
GACACAACTTGTGTAAGTGC+GGG + chr5_1:6587913-6587932 None:intergenic 45.0%
AGTTGCTTGCAGGCTTGATT+TGG - chr5_1:6588369-6588388 Msa0714020:intron 45.0%
TCCCCGGTAAATGAATACGA+AGG + chr5_1:6588448-6588467 None:intergenic 45.0%
CACGTTATTCGATGTGGACA+CGG - chr5_1:6588468-6588487 Msa0714020:intron 45.0%
!! TGAACGGGTTGGAAAGCTAA+TGG - chr5_1:6588588-6588607 Msa0714020:intron 45.0%
TTGCATGACTGCATGCATAG+AGG + chr5_1:6588839-6588858 None:intergenic 45.0%
GCAATGACTATGAGGGGATT+CGG - chr5_1:6589137-6589156 Msa0714020:CDS 45.0%
TGTTATGTTGTTCCCACGAG+AGG + chr5_1:6589166-6589185 None:intergenic 45.0%
AGAGAACTTGGACAACGGTT+TGG + chr5_1:6589688-6589707 None:intergenic 45.0%
! GCTTTCTCCTTTTCCACGTT+TGG - chr5_1:6590051-6590070 Msa0714020:CDS 45.0%
! TCTCCTTTTCCACGTTTGGA+TGG - chr5_1:6590055-6590074 Msa0714020:CDS 45.0%
CGACGACACTCTATTTCCTA+AGG - chr5_1:6590118-6590137 Msa0714020:five_prime_UTR 45.0%
GGGAACCATATTGTAAAGGG+TGG - chr5_1:6590234-6590253 Msa0714020:five_prime_UTR 45.0%
CACCACCACCCTTTACAATA+TGG + chr5_1:6590242-6590261 None:intergenic 45.0%
ATGAGAGTAGTACACCAGCA+AGG + chr5_1:6590304-6590323 None:intergenic 45.0%
!!! TTTAAATAGATTTTTTTAAA+AGG + chr5_1:6588781-6588800 None:intergenic 5.0%
!!! TTTTAAAAAAATCTATTTAA+AGG - chr5_1:6588780-6588799 Msa0714020:intron 5.0%
GAGTTCAACGAAGGACACGT+CGG + chr5_1:6587100-6587119 None:intergenic 50.0%
GAACTCTCTGATGTTCCTGG+TGG - chr5_1:6587114-6587133 Msa0714020:intron 50.0%
TCAAATGCTTCTGCACCACC+AGG + chr5_1:6587132-6587151 None:intergenic 50.0%
!! AGAAGTGATAGCGGCTCTGA+CGG - chr5_1:6587450-6587469 Msa0714020:intron 50.0%
TCAGCCCACCAATCAACAAC+TGG + chr5_1:6587510-6587529 None:intergenic 50.0%
CACATCGAATAACGTGTCCC+CGG + chr5_1:6588464-6588483 None:intergenic 50.0%
TCGATGTGGACACGGTTCAA+AGG - chr5_1:6588476-6588495 Msa0714020:intron 50.0%
GCGGTCCGATCTTCATTCAA+TGG + chr5_1:6589033-6589052 None:intergenic 50.0%
CACATGCAGCTCAAAACGAC+CGG - chr5_1:6589654-6589673 Msa0714020:intron 50.0%
!! CAACGGTTTGGACTGGTAAC+CGG + chr5_1:6589676-6589695 None:intergenic 50.0%
! ACTTGGACAACGGTTTGGAC+TGG + chr5_1:6589683-6589702 None:intergenic 50.0%
CGACTTCGCGATGCAATGAA+TGG - chr5_1:6589722-6589741 Msa0714020:intron 50.0%
!! GAGTGTCGTCGATTCTTGCT+TGG + chr5_1:6590109-6590128 None:intergenic 50.0%
GGATTGCATTGCAACCTTGC+TGG - chr5_1:6590287-6590306 Msa0714020:five_prime_UTR 50.0%
!! GTACTTGCCATGGTGGATCG+TGG - chr5_1:6586396-6586415 Msa0714020:CDS 55.0%
GTCTAGACCACGATCCACCA+TGG + chr5_1:6586406-6586425 None:intergenic 55.0%
CGGGGACACGTTATTCGATG+TGG - chr5_1:6588462-6588481 Msa0714020:intron 55.0%
GGGATTCGGATACCTCTCGT+GGG - chr5_1:6589151-6589170 Msa0714020:CDS 55.0%
GGGGATTCGGATACCTCTCG+TGG - chr5_1:6589150-6589169 Msa0714020:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 6586064 6590422 6586064 ID=Msa0714020;Name=Msa0714020
chr5_1 mRNA 6586064 6590422 6586064 ID=Msa0714020-mRNA-1;Parent=Msa0714020;Name=Msa0714020-mRNA-1;_AED=0.02;_eAED=0.02;_QI=341|1|1|1|1|1|5|284|630
chr5_1 exon 6586064 6586914 6586064 ID=Msa0714020-mRNA-1:exon:2779;Parent=Msa0714020-mRNA-1
chr5_1 exon 6587742 6588278 6587742 ID=Msa0714020-mRNA-1:exon:2778;Parent=Msa0714020-mRNA-1
chr5_1 exon 6588830 6589447 6588830 ID=Msa0714020-mRNA-1:exon:2777;Parent=Msa0714020-mRNA-1
chr5_1 exon 6589780 6589849 6589780 ID=Msa0714020-mRNA-1:exon:2776;Parent=Msa0714020-mRNA-1
chr5_1 exon 6589981 6590422 6589981 ID=Msa0714020-mRNA-1:exon:2775;Parent=Msa0714020-mRNA-1
chr5_1 five_prime_UTR 6590082 6590422 6590082 ID=Msa0714020-mRNA-1:five_prime_utr;Parent=Msa0714020-mRNA-1
chr5_1 CDS 6589981 6590081 6589981 ID=Msa0714020-mRNA-1:cds;Parent=Msa0714020-mRNA-1
chr5_1 CDS 6589780 6589849 6589780 ID=Msa0714020-mRNA-1:cds;Parent=Msa0714020-mRNA-1
chr5_1 CDS 6588830 6589447 6588830 ID=Msa0714020-mRNA-1:cds;Parent=Msa0714020-mRNA-1
chr5_1 CDS 6587742 6588278 6587742 ID=Msa0714020-mRNA-1:cds;Parent=Msa0714020-mRNA-1
chr5_1 CDS 6586348 6586914 6586348 ID=Msa0714020-mRNA-1:cds;Parent=Msa0714020-mRNA-1
chr5_1 three_prime_UTR 6586064 6586347 6586064 ID=Msa0714020-mRNA-1:three_prime_utr;Parent=Msa0714020-mRNA-1
Gene Sequence

>Msa0714020

ATGGTGGATCGTGGTCTAGACAAAACTACAACATCTGCTAGTGACTTGTCTCTGAAGAAGCAAGAGCTTCTATCTAGTGCTATGAAAAGAACAAGTGACTGGATTTTTTCTCAGGAGATTCCAAGTGATGTCAATGTCCAAATTGGAGAATCTTCCTTTACTTTACATAAGTATCCATTAGTCTCCAAGTGTGGATACATTCGGAAACTCGTCTCAGAATCTAATGACTCCGACGTGTCCTTCGTTGAACTCTCTGATGTTCCTGGTGGTGCAGAAGCATTTGAACTAGCAGCAAAATTCTGCTACGGTATAAATTTCGAGATAAATGTGGAAAACATCGCTATGCTTAGATGTGTGGCCGAATATCTTGAGATGACAGAAGATTATTCAGTTGGGAACTTGGTGGCAAGAACTGATGCATATTTGAATGATGTAGCACTAAAAACAATTGCAGGTTCTGTTTCAATCTTACATATTTCAGAAAGCCTTCTTCCAATAGCAGAGAAAACAAAATTGGTGAGCAAATGCATAGATGCAATTGCTTATATTGCTTGCAAGGAAAGTCAATTGTGTTCATCTGTAAGAAGTGATAGCGGCTCTGACGGAGTGATGTCATCTTCTGTGTCATCTCAACAGAGACCAGTTGTTGATTGGTGGGCTGAAGATCTAACTGTTCTTAGAATCGACCTTTTTCAAAGAGTTCTTATAGCAATGATGGCTAGAGGGTTTAAACAGTTTGCTATTGGTCCTATTCTAATGCTCTATGCACAGAAATCTCTACGAGGTTTGGATATATTTGGAAAGGGAAGGAAGAAGATTGAACCAAGACAAGAGCATGAAAAAAGGGTTATTTTAGAGACAATAGTGAGCCTATTACCAAAAGAGAAGAATGCAATGTCAGTAAGCTTTCTATCGATGCTGCTTCGTGCAGCAATATATCTTGATACAACAGTTGCTTGCAGGCTTGATTTGGAGAAAAGAATGGCAATGCAATTAGGACAAGCTGTCTTAGATGATCTTCTCATTCCTTCGTATTCATTTACCGGGGACACGTTATTCGATGTGGACACGGTTCAAAGGATCATGAATAATTACCAAGAATCTCAAATTGGAAACCACTTTGTCTATAATGGTGATGATGAATATTTTTCTCCACCACAAAGTGATATTGAACGGGTTGGAAAGCTAATGGAAAACTATCTTGCTGAAATAGCCACTGACAGAAATTTATCAGTTGCAAAGTTCATCAGTCTAGCTGAATTGATTCCTGAACAATCAAAACCTACAGAAGATGGGATGTATAGAGCCTTAGACATCTATTTAAAGGCTCATCCCAATTTAAGTGACATGGAGAGAAAGAAAGTTTGCAGCATAATGGACTGTCAAAGACTATCACGAGAAGCATGTGCACATGCAGCTCAAAACGACCGGTTACCAGTCCAAACCGTTGTCCAAGTTCTCTATTACGAACAGCAACGACTTCGCGATGCAATGAATGGAAGTGAAACTGCAGAATCACCTTCTATTCACACCAAACTAAATGTATATTCAGGTGATATTCAAGTTTCAAATGAACTCACAAGCTTACGAAGAGAAAATGAAGACCTCAAATTAGAGGTAGTGAAGTTGAAAATGAAACTAAAAGAGATCGAGATTTCCACGCTTAAATCAACTGTTAAATCAACGGTAAAATCAACGGTTAATAGTCCTATTGCAAATGCTTCACCTTCTCATTCTCATTCTGCTGATAAGCCTCCACTACCTCGAAAATCATTTATAAGTTCGGTTTCAAAGAAACTTGGGAAGCTTTCTCCTTTTCCACGTTTGGATGGTATTGTTACTGCTAAAGGTAGGACAAAACCAAGCAAGAATCGACGACACTCTATTTCCTAA
Protein sequence

>Msa0714020

MVDRGLDKTTTSASDLSLKKQELLSSAMKRTSDWIFSQEIPSDVNVQIGESSFTLHKYPLVSKCGYIRKLVSESNDSDVSFVELSDVPGGAEAFELAAKFCYGINFEINVENIAMLRCVAEYLEMTEDYSVGNLVARTDAYLNDVALKTIAGSVSILHISESLLPIAEKTKLVSKCIDAIAYIACKESQLCSSVRSDSGSDGVMSSSVSSQQRPVVDWWAEDLTVLRIDLFQRVLIAMMARGFKQFAIGPILMLYAQKSLRGLDIFGKGRKKIEPRQEHEKRVILETIVSLLPKEKNAMSVSFLSMLLRAAIYLDTTVACRLDLEKRMAMQLGQAVLDDLLIPSYSFTGDTLFDVDTVQRIMNNYQESQIGNHFVYNGDDEYFSPPQSDIERVGKLMENYLAEIATDRNLSVAKFISLAELIPEQSKPTEDGMYRALDIYLKAHPNLSDMERKKVCSIMDCQRLSREACAHAAQNDRLPVQTVVQVLYYEQQRLRDAMNGSETAESPSIHTKLNVYSGDIQVSNELTSLRRENEDLKLEVVKLKMKLKEIEISTLKSTVKSTVKSTVNSPIANASPSHSHSADKPPLPRKSFISSVSKKLGKLSPFPRLDGIVTAKGRTKPSKNRRHSIS*