AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1245800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1245800 MtrunA17_Chr8g0347101 97.035 371 11 0 1 371 1 371 0.0 736
Msa1245800 MtrunA17_Chr3g0126611 41.643 353 191 9 17 359 29 376 3.78e-82 255
Msa1245800 MtrunA17_Chr8g0388231 39.155 355 187 11 26 359 36 382 1.44e-59 197
Msa1245800 MtrunA17_Chr7g0275601 55.357 168 56 5 202 362 1 156 8.91e-52 170
Msa1245800 MtrunA17_Chr8g0388201 34.146 369 202 9 26 362 36 395 1.77e-50 174
Msa1245800 MtrunA17_Chr5g0396601 33.613 357 202 13 30 359 47 395 8.49e-48 166
Msa1245800 MtrunA17_Chr5g0396611 32.581 399 215 19 1 359 1 385 1.72e-40 147
Msa1245800 MtrunA17_Chr3g0081611 46.667 150 65 2 227 366 16 160 3.00e-38 135
Msa1245800 MtrunA17_Chr5g0396591 35.249 261 154 10 108 359 31 285 2.70e-35 130
Msa1245800 MtrunA17_Chr1g0160731 36.015 261 124 13 1 243 8 243 6.56e-29 118
Msa1245800 MtrunA17_Chr1g0160671 33.054 239 128 11 3 222 10 235 1.18e-25 104
Msa1245800 MtrunA17_Chr1g0160581 29.693 293 164 13 1 266 1 278 9.64e-25 105
Msa1245800 MtrunA17_Chr1g0160771 32.661 248 123 11 26 255 40 261 9.58e-24 102
Msa1245800 MtrunA17_Chr7g0248831 42.857 98 54 2 275 371 69 165 1.41e-19 85.5
Msa1245800 MtrunA17_Chr1g0177041 39.583 96 56 2 278 371 65 160 2.00e-19 84.7
Msa1245800 MtrunA17_Chr1g0158651 30.453 243 132 11 26 256 53 270 5.85e-19 88.6
Msa1245800 MtrunA17_Chr8g0348151 39.806 103 58 1 269 371 73 171 9.40e-19 83.6
Msa1245800 MtrunA17_Chr1g0158771 31.696 224 124 9 33 248 45 247 1.83e-16 80.9
Msa1245800 MtrunA17_Chr1g0197031 30.159 126 81 4 243 364 30 152 1.84e-16 76.3
Msa1245800 MtrunA17_Chr7g0248841 36.735 98 59 2 272 366 59 156 8.89e-16 74.3
Msa1245800 MtrunA17_Chr1g0158531 29.310 232 129 12 33 251 37 246 1.19e-15 78.6
Msa1245800 MtrunA17_Chr4g0037001 27.500 240 143 9 32 253 44 270 1.30e-15 78.2
Msa1245800 MtrunA17_Chr7g0273051 38.400 125 74 2 242 363 45 169 4.26e-15 75.9
Msa1245800 MtrunA17_Chr1g0197021 42.857 70 37 1 298 364 80 149 1.70e-14 70.5
Msa1245800 MtrunA17_Chr5g0409211 33.333 135 82 4 233 364 15 144 2.32e-14 70.5
Msa1245800 MtrunA17_Chr5g0399521 31.098 164 97 7 33 189 29 183 5.33e-14 71.2
Msa1245800 MtrunA17_Chr1g0157971 27.935 247 148 10 17 247 24 256 7.81e-14 72.8
Msa1245800 MtrunA17_Chr3g0113811 32.847 137 78 5 226 358 24 150 8.56e-14 68.9
Msa1245800 MtrunA17_Chr5g0409201 51.923 52 25 0 312 363 91 142 8.72e-14 68.6
Msa1245800 MtrunA17_Chr1g0158181 28.685 251 140 11 26 255 56 288 1.28e-13 72.4
Msa1245800 MtrunA17_Chr5g0426881 41.176 85 49 1 286 369 114 198 1.42e-13 71.2
Msa1245800 MtrunA17_Chr1g0158621 41.121 107 51 5 26 121 36 141 1.79e-13 67.8
Msa1245800 MtrunA17_Chr2g0300331 35.294 102 56 2 278 370 125 225 2.60e-13 68.9
Msa1245800 MtrunA17_Chr1g0191601 33.333 138 65 4 242 363 50 176 6.90e-13 69.3
Msa1245800 MtrunA17_Chr6g0486651 27.742 155 91 6 206 357 26 162 7.59e-13 69.3
Msa1245800 MtrunA17_Chr4g0027761 45.070 71 34 1 286 356 100 165 1.24e-12 66.2
Msa1245800 MtrunA17_Chr2g0295571 42.667 75 41 2 282 356 89 161 3.22e-12 66.6
Msa1245800 MtrunA17_Chr3g0133001 39.726 73 40 2 285 356 129 198 3.33e-12 67.4
Msa1245800 MtrunA17_Chr8g0345381 37.879 66 41 0 301 366 40 105 3.88e-12 63.5
Msa1245800 MtrunA17_Chr3g0126931 40.964 83 37 2 280 357 95 170 4.21e-12 66.6
Msa1245800 MtrunA17_Chr7g0246761 55.319 47 21 0 310 356 86 132 4.67e-12 64.3
Msa1245800 MtrunA17_Chr1g0186661 33.621 116 73 2 247 358 60 175 4.67e-12 66.6
Msa1245800 MtrunA17_Chr8g0336881 43.077 65 37 0 303 367 94 158 4.77e-12 66.2
Msa1245800 MtrunA17_Chr5g0426781 32.558 129 69 4 243 356 71 196 5.42e-12 66.2
Msa1245800 MtrunA17_Chr7g0274711 41.096 73 42 1 286 357 67 139 6.20e-12 63.9
Msa1245800 MtrunA17_Chr1g0198201 55.556 45 20 0 314 358 88 132 6.29e-12 64.7
Msa1245800 MtrunA17_Chr7g0274741 39.474 76 45 1 283 357 67 142 7.43e-12 63.5
Msa1245800 MtrunA17_Chr8g0350741 62.791 43 16 0 314 356 89 131 7.52e-12 63.5
Msa1245800 MtrunA17_Chr8g0345401 33.333 90 54 1 282 365 11 100 8.59e-12 62.4
Msa1245800 MtrunA17_Chr5g0426871 37.975 79 48 1 286 363 108 186 1.09e-11 65.9
Msa1245800 MtrunA17_Chr8g0383921 35.526 76 48 1 282 357 107 181 1.13e-11 63.5
Msa1245800 MtrunA17_Chr1g0184631 41.096 73 42 1 286 357 68 140 1.21e-11 63.2
Msa1245800 MtrunA17_Chr3g0120011 40.845 71 39 1 292 362 100 167 1.33e-11 63.5
Msa1245800 MtrunA17_Chr5g0444161 33.784 74 49 0 284 357 155 228 1.58e-11 63.9
Msa1245800 MtrunA17_Chr8g0383941 28.448 116 81 2 242 357 64 177 1.71e-11 63.2
Msa1245800 MtrunA17_Chr1g0148631 41.558 77 30 3 281 357 322 383 2.00e-11 65.1
Msa1245800 MtrunA17_Chr8g0383871 32.558 86 57 1 286 371 112 196 2.65e-11 62.8
Msa1245800 MtrunA17_Chr5g0444141 47.917 48 25 0 310 357 216 263 2.69e-11 63.5
Msa1245800 MtrunA17_Chr4g0050041 36.905 84 49 2 286 368 126 206 2.88e-11 64.3
Msa1245800 MtrunA17_Chr1g0184171 30.921 152 80 7 230 366 41 182 3.02e-11 63.9
Msa1245800 MtrunA17_Chr8g0381731 39.726 73 40 2 285 356 147 216 3.56e-11 63.5
Msa1245800 MtrunA17_Chr8g0364881 39.189 74 43 1 285 358 67 138 4.48e-11 63.5
Msa1245800 MtrunA17_Chr7g0274731 53.488 43 20 0 314 356 114 156 5.82e-11 61.2
Msa1245800 MtrunA17_Chr3g0132491 43.103 58 33 0 314 371 136 193 5.87e-11 63.5
Msa1245800 MtrunA17_Chr5g0413841 39.189 74 43 1 285 358 115 186 6.04e-11 63.2
Msa1245800 MtrunA17_Chr8g0383861 36.111 72 45 1 286 357 113 183 7.02e-11 61.6
Msa1245800 MtrunA17_Chr4g0067591 41.379 58 34 0 314 371 104 161 7.34e-11 62.4
Msa1245800 MtrunA17_Chr1g0203541 51.163 43 21 0 314 356 136 178 7.88e-11 61.2
Msa1245800 MtrunA17_Chr8g0383881 36.111 72 45 1 286 357 103 173 8.97e-11 61.2
Msa1245800 MtrunA17_Chr7g0274721 52.273 44 21 0 314 357 93 136 9.50e-11 60.1
Msa1245800 MtrunA17_Chr3g0118071 34.722 72 47 0 286 357 76 147 9.81e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1245800 AT2G46495.1 46.459 353 172 9 19 362 24 368 6.18e-95 288
Msa1245800 AT2G25410.1 44.282 341 185 5 26 361 34 374 9.97e-93 283
Msa1245800 AT2G46495.2 45.014 351 148 10 21 362 26 340 1.14e-85 263
Msa1245800 AT2G46494.1 42.249 329 179 6 36 360 41 362 2.32e-75 238
Msa1245800 AT5G53110.1 39.489 352 188 10 24 359 33 375 3.88e-75 238
Msa1245800 AT1G28040.1 42.951 305 151 9 68 358 2 297 2.12e-64 207
Msa1245800 AT5G36001.1 37.853 354 155 10 21 371 30 321 4.95e-61 199
Msa1245800 AT5G07040.1 47.260 146 74 2 226 371 9 151 1.74e-38 135
Msa1245800 AT5G36001.2 40.976 205 100 7 21 222 30 216 1.76e-32 121
Msa1245800 AT2G46493.1 34.123 211 108 5 153 360 2 184 2.81e-30 114
Msa1245800 AT2G35910.1 50.575 87 39 2 286 371 121 204 8.15e-24 98.6
Msa1245800 AT2G46160.1 44.944 89 43 1 281 363 99 187 1.45e-19 86.7
Msa1245800 AT2G46160.2 44.944 89 43 1 281 363 99 187 1.45e-19 86.7
Msa1245800 AT3G61550.1 43.299 97 46 1 283 370 96 192 2.30e-19 85.9
Msa1245800 AT5G06490.1 39.785 93 51 1 284 371 94 186 3.12e-18 82.4
Msa1245800 AT4G09100.1 42.857 77 43 1 286 361 56 132 7.14e-16 73.9
Msa1245800 AT4G09130.1 45.333 75 40 1 283 356 88 162 2.17e-13 71.2
Msa1245800 AT5G27420.1 40.426 94 52 2 282 371 91 184 2.35e-13 71.2
Msa1245800 AT2G34990.2 30.935 139 91 3 220 356 25 160 2.43e-13 70.5
Msa1245800 AT2G34990.1 30.935 139 91 3 220 356 3 138 2.46e-13 70.5
Msa1245800 AT4G09120.1 33.571 140 66 5 233 363 51 172 3.17e-13 70.5
Msa1245800 AT1G35330.1 34.328 134 75 4 241 363 47 178 4.02e-13 70.1
Msa1245800 AT3G05200.1 31.818 154 103 2 205 356 17 170 7.49e-13 69.7
Msa1245800 AT4G17905.2 30.714 140 80 2 230 356 88 223 9.39e-13 68.9
Msa1245800 AT1G22500.1 39.560 91 54 1 282 371 85 175 1.12e-12 68.9
Msa1245800 AT1G04360.1 33.333 150 85 4 221 358 32 178 1.14e-12 68.9
Msa1245800 AT4G17905.1 30.714 140 80 2 230 356 62 197 1.27e-12 68.6
Msa1245800 AT2G35000.1 31.496 127 83 2 234 356 51 177 1.46e-12 68.6
Msa1245800 AT4G09110.1 40.506 79 46 1 286 363 94 172 1.49e-12 68.2
Msa1245800 AT1G49200.1 35.227 88 56 1 270 357 93 179 2.32e-12 66.2
Msa1245800 AT1G49220.1 36.275 102 58 3 262 357 78 178 2.53e-12 66.6
Msa1245800 AT5G05810.1 42.466 73 38 2 285 356 119 188 3.05e-12 67.8
Msa1245800 AT4G40070.1 44.595 74 40 1 285 357 94 167 3.43e-12 67.4
Msa1245800 AT4G10160.1 32.414 145 91 3 219 363 9 146 3.71e-12 65.9
Msa1245800 AT4G28890.1 40.541 74 40 2 285 357 96 166 3.86e-12 67.8
Msa1245800 AT1G23980.1 39.189 74 43 1 285 358 117 188 4.49e-12 67.0
Msa1245800 AT2G42350.1 40.260 77 43 1 286 362 75 148 5.46e-12 65.1
Msa1245800 AT1G80400.1 43.939 66 35 2 298 362 338 402 8.09e-12 66.6
Msa1245800 AT1G72200.1 41.772 79 45 1 286 363 115 193 9.78e-12 66.2
Msa1245800 AT3G60220.1 36.190 105 56 3 267 371 76 169 1.65e-11 65.1
Msa1245800 AT5G43420.1 32.450 151 73 6 231 356 34 180 2.22e-11 65.1
Msa1245800 AT1G49230.1 30.252 119 79 2 242 357 57 174 2.65e-11 63.2
Msa1245800 AT4G10150.1 30.872 149 96 4 219 363 15 160 2.68e-11 63.5
Msa1245800 AT5G66070.5 31.496 127 74 3 240 357 97 219 2.86e-11 63.2
Msa1245800 AT5G66070.1 31.496 127 74 3 240 357 97 219 2.86e-11 63.2
Msa1245800 AT5G57750.1 35.065 77 41 2 285 357 94 165 2.87e-11 62.8
Msa1245800 AT2G27940.1 34.653 101 53 4 264 357 89 183 2.96e-11 63.5
Msa1245800 AT2G20030.1 39.189 74 41 2 285 357 97 167 3.11e-11 64.7
Msa1245800 AT1G33480.1 40.244 82 46 1 281 362 70 148 3.11e-11 63.5
Msa1245800 AT3G48030.2 37.975 79 46 2 285 362 101 177 4.11e-11 63.5
Msa1245800 AT3G18773.1 30.709 127 80 4 238 357 48 173 4.94e-11 62.4
Msa1245800 AT1G76410.1 31.707 123 73 3 242 357 29 147 4.98e-11 61.6
Msa1245800 AT5G66070.2 46.939 49 26 0 309 357 195 243 5.47e-11 62.8
Msa1245800 AT1G14200.1 39.706 68 37 2 289 356 87 150 5.80e-11 61.2
Msa1245800 AT1G49210.1 36.111 72 45 1 286 357 108 178 6.45e-11 62.0
Msa1245800 AT3G48030.1 37.975 79 46 2 285 362 179 255 7.33e-11 63.5
Msa1245800 AT3G16720.1 50.000 44 22 0 314 357 119 162 9.07e-11 62.8
Msa1245800 AT4G35840.1 30.833 120 76 2 245 357 115 234 9.13e-11 62.0
Msa1245800 AT3G10910.1 43.333 60 31 1 314 370 114 173 9.71e-11 60.8

Find 60 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTCGTGACTACACATTCTT+AGG 0.233890 8_2:+36007633 Msa1245800:CDS
TTGTTGCCACATAAAGAAAA+AGG 0.259554 8_2:-36007366 None:intergenic
ATTCGTTTCCCCTTTCAATT+AGG 0.271852 8_2:+36007399 Msa1245800:CDS
GGGGAGAGTCGACGTGTTCC+TGG 0.295592 8_2:+36009934 Msa1245800:CDS
TCTGATCATTGTTATTCTAA+TGG 0.310922 8_2:+36009759 Msa1245800:CDS
ATTGAAAGGGGAAACGAATT+AGG 0.326270 8_2:-36007395 None:intergenic
GGCTGTTCATCTCAGAATAT+AGG 0.339403 8_2:+36007654 Msa1245800:CDS
CCTGCAATTACTGTGCCTTC+AGG 0.344530 8_2:-36009868 None:intergenic
AACTTTGTCAATTCTCTGTC+TGG 0.345856 8_2:+36007744 Msa1245800:CDS
TGGAAACAATGCTTGCATTC+AGG 0.352153 8_2:-36010024 None:intergenic
CGACTAAAAGAAGCAATAAA+AGG 0.352176 8_2:-36007615 None:intergenic
ATCATAGCATTATGCGTTAC+TGG 0.355472 8_2:+36009730 Msa1245800:CDS
GCAGGAGAGTTACGACAAAC+AGG 0.377091 8_2:-36010090 None:intergenic
TATCCTTATTGTGGCTATCC+TGG 0.384579 8_2:+36007429 Msa1245800:CDS
CGTATGAGAAAGTAATTCTT+GGG 0.384681 8_2:+36009914 Msa1245800:CDS
AATTGTTCTGGTCTTGCAAT+TGG 0.402925 8_2:-36007795 None:intergenic
CCTGAAGGCACAGTAATTGC+AGG 0.408761 8_2:+36009868 Msa1245800:CDS
TTACCAGAAGTGCTGCTTCT+CGG 0.415149 8_2:+36009818 Msa1245800:CDS
TGACCGAGAAGCAGCACTTC+TGG 0.415992 8_2:-36009821 None:intergenic
AAACCAGGATAGCCACAATA+AGG 0.435316 8_2:-36007432 None:intergenic
ATCCAACAACATCCGTCGTT+CGG 0.437883 8_2:-36009958 None:intergenic
TCGTATGAGAAAGTAATTCT+TGG 0.447081 8_2:+36009913 Msa1245800:CDS
AGAGTTACTAAGACAGTCAA+TGG 0.462457 8_2:-36007691 None:intergenic
GACCGAACGACGGATGTTGT+TGG 0.463361 8_2:+36009956 Msa1245800:CDS
AGAATAACAATGATCAGAAC+TGG 0.467164 8_2:-36009754 None:intergenic
TTACTAGAGGCAACATTAAG+AGG 0.482755 8_2:-36010120 None:intergenic
GCTGTTCATCTCAGAATATA+GGG 0.484289 8_2:+36007655 Msa1245800:CDS
TATTGCTCACCTAATTGAAA+GGG 0.484335 8_2:-36007408 None:intergenic
GAGTTACTAAGACAGTCAAT+GGG 0.488340 8_2:-36007690 None:intergenic
ACGTAGAATTCTTCAGAGTA+AGG 0.496001 8_2:-36007498 None:intergenic
TTGAGGCTTAGAAGACGCTT+CGG 0.500208 8_2:-36007579 None:intergenic
ATTACTGTGCCTTCAGGTTG+CGG 0.500644 8_2:-36009862 None:intergenic
CGCTTCGGAAGACAATCATC+AGG 0.509427 8_2:-36007564 None:intergenic
GACAAAGTTAACTGAAGGAA+TGG 0.511761 8_2:-36007730 None:intergenic
GATTCACAATAACTGCAGTC+AGG 0.530225 8_2:-36007873 None:intergenic
GTGCAGATCTTCAATTAACA+TGG 0.544292 8_2:+36007844 Msa1245800:CDS
TTTCTCATACGATTCAATTG+TGG 0.544477 8_2:-36009902 None:intergenic
ATAGGACATCTTTACCTGGT+TGG 0.546643 8_2:-36007959 None:intergenic
ATATTGCTCACCTAATTGAA+AGG 0.548375 8_2:-36007409 None:intergenic
GGTGAGCAATATCCTTATTG+TGG 0.555316 8_2:+36007420 Msa1245800:CDS
ATGCTGATTGCATTGATGAG+TGG 0.559548 8_2:+36010046 Msa1245800:CDS
ACATTAAGAGGAGAAGGAGC+AGG 0.559825 8_2:-36010108 None:intergenic
TGTTTCTCTGATTACCAACC+AGG 0.560713 8_2:+36007945 Msa1245800:CDS
TGCTTCTCGGTCAGCACCAG+CGG 0.567276 8_2:+36009831 Msa1245800:CDS
GAGGCAACATTAAGAGGAGA+AGG 0.567930 8_2:-36010114 None:intergenic
GGTTGCGGTGATATTGCCGC+TGG 0.576865 8_2:-36009847 None:intergenic
GTGTGCTACAATGATAGAAT+AGG 0.583305 8_2:+36009790 Msa1245800:CDS
CGGATGTTGTTGGATATGCT+TGG 0.583608 8_2:+36009966 Msa1245800:CDS
GCAATATCACCGCAACCTGA+AGG 0.604360 8_2:+36009853 Msa1245800:CDS
GTACAATTTAAGTTGAAACC+AGG 0.606022 8_2:-36007447 None:intergenic
TGCAGATCTTCAATTAACAT+GGG 0.617198 8_2:+36007845 Msa1245800:CDS
AATCATTCAAACATTACTAG+AGG 0.621989 8_2:-36010133 None:intergenic
GAATTGACAAAGTTAACTGA+AGG 0.637579 8_2:-36007735 None:intergenic
ATTGCTCACCTAATTGAAAG+GGG 0.640458 8_2:-36007407 None:intergenic
ACTCATCAATGCAATCAGCA+TGG 0.642591 8_2:-36010044 None:intergenic
CGTGTTCCTGGACCGAACGA+CGG 0.652084 8_2:+36009946 Msa1245800:CDS
CAGGAGAGTTACGACAAACA+GGG 0.678810 8_2:-36010089 None:intergenic
GTATGAGAAAGTAATTCTTG+GGG 0.696201 8_2:+36009915 Msa1245800:CDS
GAATCACATCAGCTAATGTG+TGG 0.700025 8_2:+36007891 Msa1245800:CDS
AGTTACTAAGACAGTCAATG+GGG 0.726322 8_2:-36007689 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAGAAAAAAAGATTTA+AGG + chr8_2:36009079-36009098 Msa1245800:intron 10.0%
!!! TTATTTTATTTTTTTTCTGA+AGG - chr8_2:36009194-36009213 None:intergenic 10.0%
!!! TATACTTAATTTTTTTTGTC+AGG + chr8_2:36008779-36008798 Msa1245800:intron 15.0%
!! TTTGGAAATGTAATAAAAAA+GGG - chr8_2:36009559-36009578 None:intergenic 15.0%
!! ATTTGGAAATGTAATAAAAA+AGG - chr8_2:36009560-36009579 None:intergenic 15.0%
!! CTCTGTTATAAAAAATCTTT+CGG + chr8_2:36007770-36007789 Msa1245800:CDS 20.0%
!!! TTCAATATGCAAATTCTTTT+AGG + chr8_2:36007986-36008005 Msa1245800:intron 20.0%
!! GAGATAGAAATAAAACTATT+TGG + chr8_2:36008151-36008170 Msa1245800:intron 20.0%
!! AGGAATAACTAGAAAAATAT+TGG + chr8_2:36008193-36008212 Msa1245800:intron 20.0%
!!! ATTTATTCCTATTGACTTAA+GGG + chr8_2:36008272-36008291 Msa1245800:intron 20.0%
!!! CTTGCATTAATTTTTTTTGA+AGG - chr8_2:36008370-36008389 None:intergenic 20.0%
!! GTAAAGTATATGATTTGAAA+AGG - chr8_2:36008563-36008582 None:intergenic 20.0%
!! GTAAAGTATATGATTTGAAA+AGG - chr8_2:36008563-36008582 None:intergenic 20.0%
!!! GATGTTGTATTTTAGAAAAA+TGG + chr8_2:36008996-36009015 Msa1245800:intron 20.0%
!! ATTACATTTCCAAATAAATG+AGG + chr8_2:36009565-36009584 Msa1245800:intron 20.0%
!!! ACATTGATATTGATATTTTC+TGG + chr8_2:36009662-36009681 Msa1245800:intron 20.0%
! CAGGAAAGATTAAGTAAAAA+AGG - chr8_2:36007323-36007342 None:intergenic 25.0%
!!! TAGAATTGTTTTGCTATCAT+TGG - chr8_2:36007472-36007491 None:intergenic 25.0%
!! AGATTTTCTTGAAATTGTTC+TGG - chr8_2:36007810-36007829 None:intergenic 25.0%
! ATTTGCATATTGAAGAACAT+AGG - chr8_2:36007980-36007999 None:intergenic 25.0%
!!! GTATCCACAAATTACTTTTT+TGG + chr8_2:36008026-36008045 Msa1245800:intron 25.0%
! CTAACCAAAAAAGTAATTTG+TGG - chr8_2:36008033-36008052 None:intergenic 25.0%
!!! AAAAAACTACTTTCTGGTTA+GGG - chr8_2:36008061-36008080 None:intergenic 25.0%
!!! AAAAAAACTACTTTCTGGTT+AGG - chr8_2:36008062-36008081 None:intergenic 25.0%
!!! TAACCAGAAAGTAGTTTTTT+TGG + chr8_2:36008061-36008080 Msa1245800:intron 25.0%
! TAACCAAAAAAACTACTTTC+TGG - chr8_2:36008067-36008086 None:intergenic 25.0%
! ACTAGAAAAATATTGGTCAA+TGG + chr8_2:36008200-36008219 Msa1245800:intron 25.0%
!!! GATTTATTCCTATTGACTTA+AGG + chr8_2:36008271-36008290 Msa1245800:intron 25.0%
! AATGTTGCACTCTTTAAATT+TGG - chr8_2:36008430-36008449 None:intergenic 25.0%
!! AAATTTAAAGAGTGCAACAT+TGG + chr8_2:36008429-36008448 Msa1245800:intron 25.0%
! ACAATGCATTATTATATGCA+GGG - chr8_2:36008868-36008887 None:intergenic 25.0%
!! CTTTTCATTTTCAATCTAAC+AGG - chr8_2:36009220-36009239 None:intergenic 25.0%
! TGAACATAACAAAATTCTAC+TGG + chr8_2:36009450-36009469 Msa1245800:intron 25.0%
!! TGGACATTTTTACATACTTT+TGG - chr8_2:36009504-36009523 None:intergenic 25.0%
! ATAAAAAAGGGAGGATATAT+GGG - chr8_2:36009547-36009566 None:intergenic 25.0%
! AATAAAAAAGGGAGGATATA+TGG - chr8_2:36009548-36009567 None:intergenic 25.0%
! CAAATAAGACCTCATTTATT+TGG - chr8_2:36009577-36009596 None:intergenic 25.0%
!! TCTGATCATTGTTATTCTAA+TGG + chr8_2:36009759-36009778 Msa1245800:CDS 25.0%
TGTTGCCACATAAAGAAAAA+GGG - chr8_2:36007368-36007387 None:intergenic 30.0%
TTGTTGCCACATAAAGAAAA+AGG - chr8_2:36007369-36007388 None:intergenic 30.0%
TATTGCTCACCTAATTGAAA+GGG - chr8_2:36007411-36007430 None:intergenic 30.0%
ATATTGCTCACCTAATTGAA+AGG - chr8_2:36007412-36007431 None:intergenic 30.0%
!! GTACAATTTAAGTTGAAACC+AGG - chr8_2:36007450-36007469 None:intergenic 30.0%
AAGGAGAGTTTGAAAAATTG+AGG - chr8_2:36007599-36007618 None:intergenic 30.0%
CGACTAAAAGAAGCAATAAA+AGG - chr8_2:36007618-36007637 None:intergenic 30.0%
GAATTGACAAAGTTAACTGA+AGG - chr8_2:36007738-36007757 None:intergenic 30.0%
TGCAGATCTTCAATTAACAT+GGG + chr8_2:36007845-36007864 Msa1245800:CDS 30.0%
ATAGAATCCCTTAAGTCAAT+AGG - chr8_2:36008282-36008301 None:intergenic 30.0%
!!! TTTGCATGTTGGCAATTTTT+TGG + chr8_2:36008301-36008320 Msa1245800:intron 30.0%
TTTGGTAAACATTTGCAAGT+TGG + chr8_2:36008319-36008338 Msa1245800:intron 30.0%
AGTATACTTCACTAATGACA+CGG - chr8_2:36008712-36008731 None:intergenic 30.0%
TGGCTAAAATTCACCTTATA+AGG + chr8_2:36008810-36008829 Msa1245800:intron 30.0%
CAATGCATTATTATATGCAG+GGG - chr8_2:36008867-36008886 None:intergenic 30.0%
GACAATGCATTATTATATGC+AGG - chr8_2:36008869-36008888 None:intergenic 30.0%
ATCAAGTTCTTGATTTACTC+CGG - chr8_2:36008979-36008998 None:intergenic 30.0%
GAAATTATTTGTGAGCAATG+AGG + chr8_2:36009135-36009154 Msa1245800:intron 30.0%
TCTATAACAAAGGAAGAGTT+GGG - chr8_2:36009257-36009276 None:intergenic 30.0%
TGCTTGTTTGTCTATAACAA+AGG - chr8_2:36009267-36009286 None:intergenic 30.0%
TGAACAAATTCACATACAAG+TGG + chr8_2:36009343-36009362 Msa1245800:intron 30.0%
!! GTGGATTAAACAATGTCTTT+TGG + chr8_2:36009362-36009381 Msa1245800:intron 30.0%
GGAAATGTAATAAAAAAGGG+AGG - chr8_2:36009556-36009575 None:intergenic 30.0%
CCTTTATTAGTGTGGTTATA+GGG - chr8_2:36009610-36009629 None:intergenic 30.0%
TCCTTTATTAGTGTGGTTAT+AGG - chr8_2:36009611-36009630 None:intergenic 30.0%
!!! ATTTTCTGGCTTCAATTTTC+AGG + chr8_2:36009676-36009695 Msa1245800:intron 30.0%
AGAATAACAATGATCAGAAC+TGG - chr8_2:36009757-36009776 None:intergenic 30.0%
TTTCTCATACGATTCAATTG+TGG - chr8_2:36009905-36009924 None:intergenic 30.0%
TCGTATGAGAAAGTAATTCT+TGG + chr8_2:36009913-36009932 Msa1245800:CDS 30.0%
CGTATGAGAAAGTAATTCTT+GGG + chr8_2:36009914-36009933 Msa1245800:CDS 30.0%
GTATGAGAAAGTAATTCTTG+GGG + chr8_2:36009915-36009934 Msa1245800:CDS 30.0%
AGAAAAAGGGCAATCGTTTA+AGG - chr8_2:36007355-36007374 None:intergenic 35.0%
! GATTGCCCTTTTTCTTTATG+TGG + chr8_2:36007360-36007379 Msa1245800:CDS 35.0%
ATTGAAAGGGGAAACGAATT+AGG - chr8_2:36007398-36007417 None:intergenic 35.0%
ATTCGTTTCCCCTTTCAATT+AGG + chr8_2:36007399-36007418 Msa1245800:CDS 35.0%
ATTGCTCACCTAATTGAAAG+GGG - chr8_2:36007410-36007429 None:intergenic 35.0%
ACGTAGAATTCTTCAGAGTA+AGG - chr8_2:36007501-36007520 None:intergenic 35.0%
GCTGTTCATCTCAGAATATA+GGG + chr8_2:36007655-36007674 Msa1245800:CDS 35.0%
AGTTACTAAGACAGTCAATG+GGG - chr8_2:36007692-36007711 None:intergenic 35.0%
GAGTTACTAAGACAGTCAAT+GGG - chr8_2:36007693-36007712 None:intergenic 35.0%
AGAGTTACTAAGACAGTCAA+TGG - chr8_2:36007694-36007713 None:intergenic 35.0%
GACAAAGTTAACTGAAGGAA+TGG - chr8_2:36007733-36007752 None:intergenic 35.0%
AACTTTGTCAATTCTCTGTC+TGG + chr8_2:36007744-36007763 Msa1245800:CDS 35.0%
!! AATTGTTCTGGTCTTGCAAT+TGG - chr8_2:36007798-36007817 None:intergenic 35.0%
GTGCAGATCTTCAATTAACA+TGG + chr8_2:36007844-36007863 Msa1245800:CDS 35.0%
AAGGGATTCTATTTGCATGT+TGG + chr8_2:36008290-36008309 Msa1245800:intron 35.0%
!! TAAGGTGAATTTTAGCCACT+AGG - chr8_2:36008808-36008827 None:intergenic 35.0%
TCACCTTATAAGGTGAATAG+TGG + chr8_2:36008820-36008839 Msa1245800:intron 35.0%
ACACCACTATTCACCTTATA+AGG - chr8_2:36008826-36008845 None:intergenic 35.0%
! GTGACTTATAATTTGAGACC+CGG + chr8_2:36008957-36008976 Msa1245800:intron 35.0%
TCAAGTTCTTGATTTACTCC+GGG - chr8_2:36008978-36008997 None:intergenic 35.0%
GTCTATAACAAAGGAAGAGT+TGG - chr8_2:36009258-36009277 None:intergenic 35.0%
ATTAGTGTGGTTATAGGGAA+TGG - chr8_2:36009605-36009624 None:intergenic 35.0%
CCCTATAACCACACTAATAA+AGG + chr8_2:36009607-36009626 Msa1245800:intron 35.0%
ACTAATAAAGGATGCTCTCA+AGG + chr8_2:36009619-36009638 Msa1245800:intron 35.0%
ATCATAGCATTATGCGTTAC+TGG + chr8_2:36009730-36009749 Msa1245800:CDS 35.0%
GTGTGCTACAATGATAGAAT+AGG + chr8_2:36009790-36009809 Msa1245800:CDS 35.0%
TTACTAGAGGCAACATTAAG+AGG - chr8_2:36010123-36010142 None:intergenic 35.0%
GGTGAGCAATATCCTTATTG+TGG + chr8_2:36007420-36007439 Msa1245800:CDS 40.0%
TATCCTTATTGTGGCTATCC+TGG + chr8_2:36007429-36007448 Msa1245800:CDS 40.0%
AAACCAGGATAGCCACAATA+AGG - chr8_2:36007435-36007454 None:intergenic 40.0%
AGTCGTGACTACACATTCTT+AGG + chr8_2:36007633-36007652 Msa1245800:CDS 40.0%
GGCTGTTCATCTCAGAATAT+AGG + chr8_2:36007654-36007673 Msa1245800:CDS 40.0%
GATTCACAATAACTGCAGTC+AGG - chr8_2:36007876-36007895 None:intergenic 40.0%
GAATCACATCAGCTAATGTG+TGG + chr8_2:36007891-36007910 Msa1245800:CDS 40.0%
TGTTTCTCTGATTACCAACC+AGG + chr8_2:36007945-36007964 Msa1245800:CDS 40.0%
ATAGGACATCTTTACCTGGT+TGG - chr8_2:36007962-36007981 None:intergenic 40.0%
GAACATAGGACATCTTTACC+TGG - chr8_2:36007966-36007985 None:intergenic 40.0%
ACCAGTTGACTAAACGTGTT+TGG + chr8_2:36008103-36008122 Msa1245800:intron 40.0%
ACCAAACACGTTTAGTCAAC+TGG - chr8_2:36008107-36008126 None:intergenic 40.0%
TCCTTCTAACCTAAGCATGT+GGG - chr8_2:36008177-36008196 None:intergenic 40.0%
TTCCTTCTAACCTAAGCATG+TGG - chr8_2:36008178-36008197 None:intergenic 40.0%
CATGTTTATCTCAATCGCTG+AGG - chr8_2:36008231-36008250 None:intergenic 40.0%
CTCAGCGATTGAGATAAACA+TGG + chr8_2:36008229-36008248 Msa1245800:intron 40.0%
TGGCTTACGATATGTACGAA+CGG - chr8_2:36008410-36008429 None:intergenic 40.0%
GTTTGGTAAGCAATTGTCTC+TGG - chr8_2:36008475-36008494 None:intergenic 40.0%
GCTTACCAAACTAACACTCT+CGG + chr8_2:36008484-36008503 Msa1245800:intron 40.0%
!! TGATACCGAGAGTGTTAGTT+TGG - chr8_2:36008492-36008511 None:intergenic 40.0%
!!! TTTTTTGTCAGGTAGCCTAG+TGG + chr8_2:36008790-36008809 Msa1245800:intron 40.0%
ATAAGGTGAATAGTGGTGTC+CGG + chr8_2:36008827-36008846 Msa1245800:intron 40.0%
AAAGGGAGGATATATGGGTT+CGG - chr8_2:36009542-36009561 None:intergenic 40.0%
!! GAGAGCATCCTTTATTAGTG+TGG - chr8_2:36009618-36009637 None:intergenic 40.0%
TGGAAACAATGCTTGCATTC+AGG - chr8_2:36010027-36010046 None:intergenic 40.0%
ACTCATCAATGCAATCAGCA+TGG - chr8_2:36010047-36010066 None:intergenic 40.0%
! ATGCTGATTGCATTGATGAG+TGG + chr8_2:36010046-36010065 Msa1245800:CDS 40.0%
! TCGTTTAAGGCGTATACGAC+AGG - chr8_2:36007342-36007361 None:intergenic 45.0%
TTGAGGCTTAGAAGACGCTT+CGG - chr8_2:36007582-36007601 None:intergenic 45.0%
ACTATTTGGCCCACATGCTT+AGG + chr8_2:36008165-36008184 Msa1245800:intron 45.0%
TAAGGTGAATAGTGGTGTCC+GGG + chr8_2:36008828-36008847 Msa1245800:intron 45.0%
ATTATTATATGCAGGGGCCG+GGG - chr8_2:36008861-36008880 None:intergenic 45.0%
CATTATTATATGCAGGGGCC+GGG - chr8_2:36008862-36008881 None:intergenic 45.0%
GCATTATTATATGCAGGGGC+CGG - chr8_2:36008863-36008882 None:intergenic 45.0%
TTGAGCATAGCTCAGTTGGT+AGG - chr8_2:36008891-36008910 None:intergenic 45.0%
TACCAACTGAGCTATGCTCA+AGG + chr8_2:36008890-36008909 Msa1245800:intron 45.0%
ACCAACTGAGCTATGCTCAA+GGG + chr8_2:36008891-36008910 Msa1245800:intron 45.0%
!!! TTTTCAGGTACATCGCGACA+AGG + chr8_2:36009691-36009710 Msa1245800:intron 45.0%
!! TTACCAGAAGTGCTGCTTCT+CGG + chr8_2:36009818-36009837 Msa1245800:CDS 45.0%
!! ATTACTGTGCCTTCAGGTTG+CGG - chr8_2:36009865-36009884 None:intergenic 45.0%
ATCCAACAACATCCGTCGTT+CGG - chr8_2:36009961-36009980 None:intergenic 45.0%
CGGATGTTGTTGGATATGCT+TGG + chr8_2:36009966-36009985 Msa1245800:CDS 45.0%
CAGGAGAGTTACGACAAACA+GGG - chr8_2:36010092-36010111 None:intergenic 45.0%
ACATTAAGAGGAGAAGGAGC+AGG - chr8_2:36010111-36010130 None:intergenic 45.0%
GAGGCAACATTAAGAGGAGA+AGG - chr8_2:36010117-36010136 None:intergenic 45.0%
!! TAAAATAATTAAAATATTCT+AGG + chr8_2:36009028-36009047 Msa1245800:intron 5.0%
!! AAAATAATTAAAATATTCTA+GGG + chr8_2:36009029-36009048 Msa1245800:intron 5.0%
CGCTTCGGAAGACAATCATC+AGG - chr8_2:36007567-36007586 None:intergenic 50.0%
GCCCACATGCTTAGGTTAGA+AGG + chr8_2:36008173-36008192 Msa1245800:intron 50.0%
AAGGTGAATAGTGGTGTCCG+GGG + chr8_2:36008829-36008848 Msa1245800:intron 50.0%
TCCCTTGAGCATAGCTCAGT+TGG - chr8_2:36008895-36008914 None:intergenic 50.0%
TTCGGAGTTGCAGCAACTCA+TGG - chr8_2:36009524-36009543 None:intergenic 50.0%
GCAATATCACCGCAACCTGA+AGG + chr8_2:36009853-36009872 Msa1245800:CDS 50.0%
CCTGCAATTACTGTGCCTTC+AGG - chr8_2:36009871-36009890 None:intergenic 50.0%
! CCTGAAGGCACAGTAATTGC+AGG + chr8_2:36009868-36009887 Msa1245800:CDS 50.0%
GCAGGAGAGTTACGACAAAC+AGG - chr8_2:36010093-36010112 None:intergenic 50.0%
TGACCGAGAAGCAGCACTTC+TGG - chr8_2:36009824-36009843 None:intergenic 55.0%
GACCGAACGACGGATGTTGT+TGG + chr8_2:36009956-36009975 Msa1245800:CDS 55.0%
TGCTTCTCGGTCAGCACCAG+CGG + chr8_2:36009831-36009850 Msa1245800:CDS 60.0%
GGTTGCGGTGATATTGCCGC+TGG - chr8_2:36009850-36009869 None:intergenic 60.0%
CGTGTTCCTGGACCGAACGA+CGG + chr8_2:36009946-36009965 Msa1245800:CDS 60.0%
CAACATCCGTCGTTCGGTCC+AGG - chr8_2:36009955-36009974 None:intergenic 60.0%
GGGGAGAGTCGACGTGTTCC+TGG + chr8_2:36009934-36009953 Msa1245800:CDS 65.0%
GGTGTCCGGGGTTCGAACCC+CGG + chr8_2:36008841-36008860 Msa1245800:intron 70.0%
AGGGGCCGGGGTTCGAACCC+CGG - chr8_2:36008849-36008868 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 36007303 36010149 36007303 ID=Msa1245800;Name=Msa1245800
chr8_2 mRNA 36007303 36010149 36007303 ID=Msa1245800-mRNA-1;Parent=Msa1245800;Name=Msa1245800-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|2|0|371
chr8_2 exon 36007303 36007966 36007303 ID=Msa1245800-mRNA-1:exon:11433;Parent=Msa1245800-mRNA-1
chr8_2 exon 36009698 36010149 36009698 ID=Msa1245800-mRNA-1:exon:11434;Parent=Msa1245800-mRNA-1
chr8_2 CDS 36007303 36007966 36007303 ID=Msa1245800-mRNA-1:cds;Parent=Msa1245800-mRNA-1
chr8_2 CDS 36009698 36010149 36009698 ID=Msa1245800-mRNA-1:cds;Parent=Msa1245800-mRNA-1
Gene Sequence

>Msa1245800

ATGGACATCTTGAAAGTCCTTTTTTACTTAATCTTTCCTGTCGTATACGCCTTAAACGATTGCCCTTTTTCTTTATGTGGCAACAATAGTTTCCTAATTCGTTTCCCCTTTCAATTAGGTGAGCAATATCCTTATTGTGGCTATCCTGGTTTCAACTTAAATTGTACCAATGATAGCAAAACAATTCTAAAACTTCCTTACTCTGAAGAATTCTACGTACGCAGCATAAACTATCTCACACAACAGATACAACTCTATGATCCTGATGATTGTCTTCCGAAGCGTCTTCTAAGCCTCAATTTTTCAAACTCTCCTTTTATTGCTTCTTTTAGTCGTGACTACACATTCTTAGGCTGTTCATCTCAGAATATAGGGTCACAATTCATCCCCATTGACTGTCTTAGTAACTCTACATATTTTGTATCAGCCATTCCTTCAGTTAACTTTGTCAATTCTCTGTCTGGTTGCTCTGTTATAAAAAATCTTTCGGTTCCAATTGCAAGACCAGAACAATTTCAAGAAAATCTCAGAGATGATCTTAGTGCAGATCTTCAATTAACATGGGATAAACCTGACTGCAGTTATTGTGAATCACATCAGCTAATGTGTGGATTTGAAAGCATTAACAGCAATCAAGTTGTCTGTTTCTCTGATTACCAACCAGGTACATCGCGACAAGGACTTAAAATTTTCAGAATCATAGCATTATGCGTTACTGGACCAGTTCTGATCATTGTTATTCTAATGGCAAGTTGTGTGTGCTACAATGATAGAATAGGCAACATTACCAGAAGTGCTGCTTCTCGGTCAGCACCAGCGGCAATATCACCGCAACCTGAAGGCACAGTAATTGCAGGTTTAGATGAATCCACAATTGAATCGTATGAGAAAGTAATTCTTGGGGAGAGTCGACGTGTTCCTGGACCGAACGACGGATGTTGTTGGATATGCTTGGCAGAATACAACAGTAAAGAGACAGTAAGATGCATTCCTGAATGCAAGCATTGTTTCCATGCTGATTGCATTGATGAGTGGCTTCGTATGAATGTTACATGCCCTGTTTGTCGTAACTCTCCTGCTCCTTCTCCTCTTAATGTTGCCTCTAGTAATGTTTGA
Protein sequence

>Msa1245800

MDILKVLFYLIFPVVYALNDCPFSLCGNNSFLIRFPFQLGEQYPYCGYPGFNLNCTNDSKTILKLPYSEEFYVRSINYLTQQIQLYDPDDCLPKRLLSLNFSNSPFIASFSRDYTFLGCSSQNIGSQFIPIDCLSNSTYFVSAIPSVNFVNSLSGCSVIKNLSVPIARPEQFQENLRDDLSADLQLTWDKPDCSYCESHQLMCGFESINSNQVVCFSDYQPGTSRQGLKIFRIIALCVTGPVLIIVILMASCVCYNDRIGNITRSAASRSAPAAISPQPEGTVIAGLDESTIESYEKVILGESRRVPGPNDGCCWICLAEYNSKETVRCIPECKHCFHADCIDEWLRMNVTCPVCRNSPAPSPLNVASSNV*