AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0792330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0792330 MtrunA17_Chr8g0347101 97.305 371 10 0 1 371 1 371 0.0 736
Msa0792330 MtrunA17_Chr3g0126611 41.926 353 190 9 17 359 29 376 1.16e-83 259
Msa0792330 MtrunA17_Chr8g0388231 39.437 355 186 11 26 359 36 382 7.06e-60 198
Msa0792330 MtrunA17_Chr7g0275601 55.357 168 56 5 202 362 1 156 1.68e-51 169
Msa0792330 MtrunA17_Chr8g0388201 34.417 369 201 11 26 362 36 395 1.10e-49 172
Msa0792330 MtrunA17_Chr5g0396601 33.613 357 202 13 30 359 47 395 8.67e-48 166
Msa0792330 MtrunA17_Chr5g0396611 32.494 397 218 18 1 359 1 385 2.58e-40 146
Msa0792330 MtrunA17_Chr3g0081611 65.116 86 30 0 281 366 75 160 4.39e-38 134
Msa0792330 MtrunA17_Chr5g0396591 34.884 258 159 8 108 359 31 285 2.59e-35 130
Msa0792330 MtrunA17_Chr1g0160731 36.214 243 121 12 1 225 8 234 2.32e-27 113
Msa0792330 MtrunA17_Chr1g0160671 33.054 239 128 11 3 222 10 235 7.21e-25 102
Msa0792330 MtrunA17_Chr1g0160581 33.197 244 125 12 1 217 1 233 3.80e-24 103
Msa0792330 MtrunA17_Chr1g0160771 32.661 248 123 11 26 255 40 261 5.66e-23 100
Msa0792330 MtrunA17_Chr7g0248831 42.857 98 54 2 275 371 69 165 1.25e-19 85.5
Msa0792330 MtrunA17_Chr1g0177041 39.583 96 56 2 278 371 65 160 2.06e-19 84.7
Msa0792330 MtrunA17_Chr8g0348151 39.806 103 58 1 269 371 73 171 8.86e-19 84.0
Msa0792330 MtrunA17_Chr1g0158651 29.752 242 133 11 26 255 53 269 1.57e-17 84.3
Msa0792330 MtrunA17_Chr1g0197031 30.159 126 81 4 243 364 30 152 1.78e-16 76.3
Msa0792330 MtrunA17_Chr1g0158771 36.364 165 89 6 33 189 45 201 3.24e-16 80.1
Msa0792330 MtrunA17_Chr4g0037001 27.917 240 142 9 32 253 44 270 3.65e-16 80.1
Msa0792330 MtrunA17_Chr1g0158311 26.616 263 162 11 26 277 33 275 7.94e-16 79.0
Msa0792330 MtrunA17_Chr7g0248841 36.735 98 59 2 272 366 59 156 8.38e-16 74.3
Msa0792330 MtrunA17_Chr7g0273051 39.200 125 73 2 242 363 45 169 1.21e-15 77.4
Msa0792330 MtrunA17_Chr5g0409211 34.074 135 81 4 233 364 15 144 7.32e-15 72.0
Msa0792330 MtrunA17_Chr1g0197021 42.857 70 37 1 298 364 80 149 1.86e-14 70.5
Msa0792330 MtrunA17_Chr1g0157971 30.556 216 123 9 17 216 24 228 1.91e-14 74.7
Msa0792330 MtrunA17_Chr1g0158531 28.879 232 130 12 33 251 37 246 2.17e-14 74.7
Msa0792330 MtrunA17_Chr2g0300331 31.724 145 79 4 235 370 92 225 7.31e-14 70.5
Msa0792330 MtrunA17_Chr5g0409201 51.923 52 25 0 312 363 91 142 9.62e-14 68.6
Msa0792330 MtrunA17_Chr5g0399521 31.098 164 97 7 33 189 29 183 1.31e-13 70.1
Msa0792330 MtrunA17_Chr5g0426881 41.176 85 49 1 286 369 114 198 1.50e-13 71.2
Msa0792330 MtrunA17_Chr1g0158621 41.121 107 51 5 26 121 36 141 1.62e-13 67.8
Msa0792330 MtrunA17_Chr3g0113811 32.847 137 78 5 226 358 24 150 1.79e-13 68.2
Msa0792330 MtrunA17_Chr1g0191601 34.058 138 64 4 242 363 50 176 2.20e-13 70.9
Msa0792330 MtrunA17_Chr6g0486651 28.758 153 92 5 206 357 26 162 4.16e-13 70.1
Msa0792330 MtrunA17_Chr4g0027761 45.070 71 34 1 286 356 100 165 1.29e-12 66.2
Msa0792330 MtrunA17_Chr2g0295571 42.667 75 41 2 282 356 89 161 3.19e-12 66.6
Msa0792330 MtrunA17_Chr3g0133001 39.726 73 40 2 285 356 129 198 3.74e-12 67.4
Msa0792330 MtrunA17_Chr3g0126931 40.964 83 37 2 280 357 95 170 4.02e-12 67.0
Msa0792330 MtrunA17_Chr8g0345381 37.879 66 41 0 301 366 40 105 4.19e-12 63.5
Msa0792330 MtrunA17_Chr7g0246761 55.319 47 21 0 310 356 86 132 4.67e-12 64.3
Msa0792330 MtrunA17_Chr1g0186661 33.621 116 73 2 247 358 60 175 4.76e-12 66.6
Msa0792330 MtrunA17_Chr8g0336881 30.000 140 93 2 228 367 24 158 4.86e-12 66.2
Msa0792330 MtrunA17_Chr5g0426781 32.558 129 69 4 243 356 71 196 5.32e-12 66.6
Msa0792330 MtrunA17_Chr7g0274711 41.096 73 42 1 286 357 67 139 6.02e-12 63.9
Msa0792330 MtrunA17_Chr1g0198201 55.556 45 20 0 314 358 88 132 6.06e-12 64.7
Msa0792330 MtrunA17_Chr7g0274741 39.474 76 45 1 283 357 67 142 7.15e-12 63.5
Msa0792330 MtrunA17_Chr8g0350741 62.791 43 16 0 314 356 89 131 7.59e-12 63.5
Msa0792330 MtrunA17_Chr8g0345401 33.333 90 54 1 282 365 11 100 7.95e-12 62.8
Msa0792330 MtrunA17_Chr5g0426871 37.975 79 48 1 286 363 108 186 1.13e-11 65.9
Msa0792330 MtrunA17_Chr8g0383921 35.526 76 48 1 282 357 107 181 1.19e-11 63.5
Msa0792330 MtrunA17_Chr3g0120011 40.845 71 39 1 292 362 100 167 1.22e-11 63.5
Msa0792330 MtrunA17_Chr1g0184631 41.096 73 42 1 286 357 68 140 1.23e-11 63.2
Msa0792330 MtrunA17_Chr5g0444161 32.927 82 54 1 284 365 155 235 1.32e-11 63.9
Msa0792330 MtrunA17_Chr1g0148631 41.558 77 30 3 281 357 322 383 2.20e-11 64.7
Msa0792330 MtrunA17_Chr5g0444141 47.917 48 25 0 310 357 216 263 2.43e-11 63.5
Msa0792330 MtrunA17_Chr8g0383871 32.558 86 57 1 286 371 112 196 2.72e-11 62.8
Msa0792330 MtrunA17_Chr4g0050041 36.905 84 49 2 286 368 126 206 2.83e-11 64.3
Msa0792330 MtrunA17_Chr8g0381731 39.726 73 40 2 285 356 147 216 3.66e-11 63.5
Msa0792330 MtrunA17_Chr8g0364881 39.189 74 43 1 285 358 67 138 4.81e-11 63.5
Msa0792330 MtrunA17_Chr8g0383941 28.696 115 80 2 243 357 65 177 5.15e-11 62.0
Msa0792330 MtrunA17_Chr3g0132491 34.483 87 56 1 286 371 107 193 5.21e-11 63.5
Msa0792330 MtrunA17_Chr7g0274731 53.488 43 20 0 314 356 114 156 5.65e-11 61.2
Msa0792330 MtrunA17_Chr1g0184171 38.824 85 43 3 286 366 103 182 6.26e-11 63.2
Msa0792330 MtrunA17_Chr5g0413841 39.189 74 43 1 285 358 115 186 6.43e-11 63.2
Msa0792330 MtrunA17_Chr4g0067591 41.379 58 34 0 314 371 104 161 6.88e-11 62.4
Msa0792330 MtrunA17_Chr8g0383861 36.111 72 45 1 286 357 113 183 6.89e-11 61.6
Msa0792330 MtrunA17_Chr1g0203541 51.163 43 21 0 314 356 136 178 7.31e-11 61.2
Msa0792330 MtrunA17_Chr8g0383881 36.111 72 45 1 286 357 103 173 9.05e-11 61.2
Msa0792330 MtrunA17_Chr3g0118071 34.722 72 47 0 286 357 76 147 9.09e-11 61.2
Msa0792330 MtrunA17_Chr7g0274721 52.273 44 21 0 314 357 93 136 9.50e-11 60.1
Msa0792330 MtrunA17_Chr8g0369571 28.667 150 91 5 217 364 5 140 9.58e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0792330 AT2G46495.1 46.742 353 171 9 19 362 24 368 2.32e-94 286
Msa0792330 AT2G25410.1 43.988 341 186 5 26 361 34 374 5.36e-91 278
Msa0792330 AT2G46495.2 45.042 353 149 10 19 362 24 340 1.41e-85 263
Msa0792330 AT5G53110.1 39.489 352 188 10 24 359 33 375 1.29e-75 239
Msa0792330 AT2G46494.1 42.249 329 179 6 36 360 41 362 2.09e-75 238
Msa0792330 AT1G28040.1 43.189 301 156 9 68 358 2 297 3.03e-64 207
Msa0792330 AT5G36001.1 37.853 354 155 10 21 371 30 321 1.44e-60 198
Msa0792330 AT5G07040.1 46.575 146 75 2 226 371 9 151 8.41e-38 134
Msa0792330 AT5G36001.2 40.976 205 100 7 21 222 30 216 3.96e-32 120
Msa0792330 AT2G46493.1 34.597 211 107 5 153 360 2 184 2.38e-31 117
Msa0792330 AT2G35910.1 50.575 87 39 2 286 371 121 204 8.57e-24 98.2
Msa0792330 AT2G46160.1 44.944 89 43 1 281 363 99 187 1.54e-19 86.7
Msa0792330 AT2G46160.2 44.944 89 43 1 281 363 99 187 1.54e-19 86.7
Msa0792330 AT3G61550.1 43.299 97 46 1 283 370 96 192 2.12e-19 86.3
Msa0792330 AT5G06490.1 39.785 93 51 1 284 371 94 186 3.25e-18 82.4
Msa0792330 AT4G09100.1 42.857 77 43 1 286 361 56 132 7.14e-16 73.9
Msa0792330 AT4G09120.1 34.286 140 65 5 233 363 51 172 9.11e-14 72.0
Msa0792330 AT1G35330.1 35.075 134 74 4 241 363 47 178 1.25e-13 71.6
Msa0792330 AT5G27420.1 40.426 94 52 2 282 371 91 184 2.05e-13 71.2
Msa0792330 AT4G09130.1 45.333 75 40 1 283 356 88 162 2.23e-13 71.2
Msa0792330 AT2G34990.2 30.435 138 93 3 220 356 25 160 2.74e-13 70.5
Msa0792330 AT2G34990.1 30.435 138 93 3 220 356 3 138 2.91e-13 70.1
Msa0792330 AT3G05200.1 31.250 160 96 4 205 356 17 170 5.60e-13 70.1
Msa0792330 AT1G22500.1 39.560 91 54 1 282 371 85 175 1.09e-12 69.3
Msa0792330 AT4G09110.1 40.506 79 46 1 286 363 94 172 1.51e-12 68.2
Msa0792330 AT1G04360.1 35.099 151 81 6 221 358 32 178 1.52e-12 68.6
Msa0792330 AT4G17905.2 30.714 140 80 2 230 356 88 223 2.07e-12 68.2
Msa0792330 AT1G49200.1 35.227 88 56 1 270 357 93 179 2.25e-12 66.6
Msa0792330 AT2G35000.1 31.496 127 83 2 234 356 51 177 2.28e-12 68.2
Msa0792330 AT1G49220.1 36.275 102 58 3 262 357 78 178 2.78e-12 66.6
Msa0792330 AT4G17905.1 30.714 140 80 2 230 356 62 197 2.93e-12 67.4
Msa0792330 AT5G05810.1 39.506 81 45 2 285 364 119 196 3.19e-12 67.8
Msa0792330 AT4G40070.1 44.595 74 40 1 285 357 94 167 3.34e-12 67.4
Msa0792330 AT4G28890.1 40.541 74 40 2 285 357 96 166 4.19e-12 67.4
Msa0792330 AT2G42350.1 40.260 77 43 1 286 362 75 148 5.01e-12 65.1
Msa0792330 AT1G23980.1 39.189 74 43 1 285 358 117 188 5.29e-12 67.0
Msa0792330 AT4G10160.1 41.463 82 45 1 282 363 68 146 6.08e-12 65.1
Msa0792330 AT1G80400.1 43.939 66 35 2 298 362 338 402 8.02e-12 66.6
Msa0792330 AT1G72200.1 41.772 79 45 1 286 363 115 193 9.35e-12 66.2
Msa0792330 AT3G60220.1 36.190 105 56 3 267 371 76 169 1.66e-11 65.1
Msa0792330 AT5G66070.5 33.333 108 62 2 256 357 116 219 2.43e-11 63.2
Msa0792330 AT5G66070.1 33.333 108 62 2 256 357 116 219 2.43e-11 63.2
Msa0792330 AT3G48030.2 30.137 146 83 5 230 362 38 177 2.91e-11 63.9
Msa0792330 AT4G10150.1 40.244 82 46 1 282 363 82 160 2.92e-11 63.5
Msa0792330 AT2G27940.1 34.653 101 53 4 264 357 89 183 3.01e-11 63.2
Msa0792330 AT5G57750.1 34.615 78 42 2 285 358 94 166 3.06e-11 62.8
Msa0792330 AT1G33480.1 40.244 82 46 1 281 362 70 148 3.17e-11 63.5
Msa0792330 AT5G43420.1 31.788 151 74 6 231 356 34 180 3.22e-11 64.7
Msa0792330 AT2G20030.1 39.189 74 41 2 285 357 97 167 3.46e-11 64.7
Msa0792330 AT5G66070.2 46.939 49 26 0 309 357 195 243 5.36e-11 62.8
Msa0792330 AT1G14200.1 39.706 68 37 2 289 356 87 150 5.92e-11 61.2
Msa0792330 AT1G49210.1 36.111 72 45 1 286 357 108 178 6.15e-11 62.4
Msa0792330 AT4G35840.1 30.833 120 76 2 245 357 115 234 7.71e-11 62.0
Msa0792330 AT3G48030.1 37.975 79 46 2 285 362 179 255 7.96e-11 63.2
Msa0792330 AT3G16720.1 50.000 44 22 0 314 357 119 162 8.59e-11 62.8
Msa0792330 AT1G49230.1 36.986 73 45 1 285 357 103 174 9.10e-11 61.6
Msa0792330 AT3G10910.1 43.333 60 31 1 314 370 114 173 9.81e-11 60.8

Find 66 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTGCCACATAAAGAAAA+AGG 0.259554 5_3:-3462501 None:intergenic
ATTCGTTTCCCCTTTCAATT+AGG 0.271852 5_3:+3462534 Msa0792330:CDS
AGTCGTGACTACACCTTCTT+AGG 0.282841 5_3:+3462768 Msa0792330:CDS
GGGGAGAGTCGACGTGTTCC+TGG 0.295592 5_3:+3465076 Msa0792330:CDS
ATTGAAAGGGGAAACGAATT+AGG 0.326270 5_3:-3462530 None:intergenic
TCTGATCATTGTTATTCTAA+TGG 0.337767 5_3:+3464901 Msa0792330:CDS
GGCTGTTCATCTCAGAATAT+AGG 0.339403 5_3:+3462789 Msa0792330:CDS
ATTGAGGCTTAGAAGACGTT+TGG 0.339581 5_3:-3462715 None:intergenic
CCTGCAATTACTGTGCCTTC+AGG 0.344530 5_3:-3465010 None:intergenic
TGGAAACAATGCTTGCATTC+AGG 0.352153 5_3:-3465166 None:intergenic
CGACTAAAAGAAGCAATAAA+AGG 0.352176 5_3:-3462750 None:intergenic
ATCATAGCATTATGCGTTAC+TGG 0.355472 5_3:+3464872 Msa0792330:CDS
AGCTTTGTCAATTCTCTGTC+TGG 0.363370 5_3:+3462879 Msa0792330:CDS
AACCGTTCTGGTCTTGCAAT+TGG 0.365731 5_3:-3462930 None:intergenic
GCAGGAGAGTTACGACAAAC+AGG 0.377091 5_3:-3465232 None:intergenic
TATCCTTATTGTGGCTATCC+TGG 0.384579 5_3:+3462564 Msa0792330:CDS
CGTATGAGAAAGTAATTCTT+GGG 0.384681 5_3:+3465056 Msa0792330:CDS
CCTGAAGGCACAGTAATTGC+AGG 0.408761 5_3:+3465010 Msa0792330:CDS
TTACCAGAAGTGCTGCTTCT+CGG 0.415149 5_3:+3464960 Msa0792330:CDS
TGACCGAGAAGCAGCACTTC+TGG 0.415992 5_3:-3464963 None:intergenic
TTGAGGCTTAGAAGACGTTT+GGG 0.423213 5_3:-3462714 None:intergenic
AGAGTTACTAAGACAATCAA+TGG 0.428456 5_3:-3462826 None:intergenic
TCGTTTAAGGCGTATGCGAC+AGG 0.432440 5_3:-3462474 None:intergenic
AAACCAGGATAGCCACAATA+AGG 0.435316 5_3:-3462567 None:intergenic
ATCCAACAACATCCGTCGTT+CGG 0.437883 5_3:-3465100 None:intergenic
TCGTATGAGAAAGTAATTCT+TGG 0.447081 5_3:+3465055 Msa0792330:CDS
GACCGAACGACGGATGTTGT+TGG 0.463361 5_3:+3465098 Msa0792330:CDS
TTACTAGAGGCAACATTAAG+AGG 0.482755 5_3:-3465262 None:intergenic
GCTGTTCATCTCAGAATATA+GGG 0.484289 5_3:+3462790 Msa0792330:CDS
TATTGCTCACCTAATTGAAA+GGG 0.484335 5_3:-3462543 None:intergenic
ATCTAACATCTTTACCTGGT+TGG 0.489784 5_3:-3463094 None:intergenic
ACATAGAATTCTTCAGAGTA+AGG 0.498143 5_3:-3462633 None:intergenic
ATTACTGTGCCTTCAGGTTG+CGG 0.500644 5_3:-3465004 None:intergenic
GACAAAGCTAACTGAAGGAA+TGG 0.510349 5_3:-3462865 None:intergenic
ACGTTTGGGAAGACAATCAT+CGG 0.534356 5_3:-3462700 None:intergenic
GTGCAGATCTTCAATTAACA+TGG 0.544292 5_3:+3462979 Msa0792330:CDS
TTTCTCATACGATTCAATTG+TGG 0.544477 5_3:-3465044 None:intergenic
ATATTGCTCACCTAATTGAA+AGG 0.548375 5_3:-3462544 None:intergenic
CTGAGATGAACAGCCTAAGA+AGG 0.553624 5_3:-3462781 None:intergenic
GATTCACAATAACTGCAGTC+AGG 0.554160 5_3:-3463008 None:intergenic
GGTGAGCAATATCCTTATTG+TGG 0.555316 5_3:+3462555 Msa0792330:CDS
ATGCTGATTGCATTGATGAG+TGG 0.559548 5_3:+3465188 Msa0792330:CDS
ACATTAAGAGGAGAAGGAGC+AGG 0.559825 5_3:-3465250 None:intergenic
TGTTTCTCTGATTACCAACC+AGG 0.560713 5_3:+3463080 Msa0792330:CDS
TGCTTCTCGGTCAGCACCAG+CGG 0.567276 5_3:+3464973 Msa0792330:CDS
AGAATAACAATGATCAGAGC+TGG 0.567847 5_3:-3464896 None:intergenic
GAGGCAACATTAAGAGGAGA+AGG 0.567930 5_3:-3465256 None:intergenic
TTAATTGAAGATCTGCACTA+AGG 0.568451 5_3:-3462974 None:intergenic
CGTTTGGGAAGACAATCATC+GGG 0.574221 5_3:-3462699 None:intergenic
GGTTGCGGTGATATTGCCGC+TGG 0.576865 5_3:-3464989 None:intergenic
GTGTGCTACAATGATAGAAT+AGG 0.583305 5_3:+3464932 Msa0792330:CDS
CGGATGTTGTTGGATATGCT+TGG 0.583608 5_3:+3465108 Msa0792330:CDS
GAGTTACTAAGACAATCAAT+GGG 0.593741 5_3:-3462825 None:intergenic
GTCTTGCAATTGGAACTGAA+AGG 0.594777 5_3:-3462920 None:intergenic
GCAATATCACCGCAACCTGA+AGG 0.604360 5_3:+3464995 Msa0792330:CDS
GTACAATTTAAGTTGAAACC+AGG 0.606022 5_3:-3462582 None:intergenic
TGCAGATCTTCAATTAACAT+GGG 0.617198 5_3:+3462980 Msa0792330:CDS
AATCATTCAAACATTACTAG+AGG 0.621989 5_3:-3465275 None:intergenic
GAATTGACAAAGCTAACTGA+AGG 0.634686 5_3:-3462870 None:intergenic
ATTGCTCACCTAATTGAAAG+GGG 0.640458 5_3:-3462542 None:intergenic
TTCCAATTGCAAGACCAGAA+CGG 0.642475 5_3:+3462928 Msa0792330:CDS
ACTCATCAATGCAATCAGCA+TGG 0.642591 5_3:-3465186 None:intergenic
CGTGTTCCTGGACCGAACGA+CGG 0.652084 5_3:+3465088 Msa0792330:CDS
CAGGAGAGTTACGACAAACA+GGG 0.678810 5_3:-3465231 None:intergenic
GTATGAGAAAGTAATTCTTG+GGG 0.696201 5_3:+3465057 Msa0792330:CDS
GAATCGCATCAGCTAATGTG+TGG 0.708361 5_3:+3463026 Msa0792330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAAAAAATAAAACTAGT+TGG + chr5_3:3463325-3463344 Msa0792330:intron 10.0%
!! ATAAAAGAAAAAAAGATTTA+AGG + chr5_3:3464227-3464246 Msa0792330:intron 10.0%
!!! TTAATATTTTATTGCTTTTT+TGG - chr5_3:3464423-3464442 None:intergenic 10.0%
!! AAAAAGCAATAAAATATTAA+CGG + chr5_3:3464423-3464442 Msa0792330:intron 10.0%
!!! GTATTTTTTTTTTTTTCTGA+AGG - chr5_3:3464342-3464361 None:intergenic 15.0%
!! TTTGGAAATGTAATAAAAAA+GGG - chr5_3:3464701-3464720 None:intergenic 15.0%
!! ATTTGGAAATGTAATAAAAA+AGG - chr5_3:3464702-3464721 None:intergenic 15.0%
!!! TATCCTGAAATTAGTTTTTT+TGG + chr5_3:3463168-3463187 Msa0792330:intron 20.0%
!! TAACCAAAAAAACTAATTTC+AGG - chr5_3:3463174-3463193 None:intergenic 20.0%
!!! ATATCCAGAAATTAGTTTTT+TGG + chr5_3:3463202-3463221 Msa0792330:intron 20.0%
!!! TATCCAGAAATTAGTTTTTT+TGG + chr5_3:3463203-3463222 Msa0792330:intron 20.0%
!! TAACCAAAAAAACTAATTTC+TGG - chr5_3:3463174-3463193 None:intergenic 20.0%
!! ATATAATACCTTAAGTCAAT+AGG - chr5_3:3463457-3463476 None:intergenic 20.0%
!!! AAGGTATTATATTTGCATAT+TGG + chr5_3:3463465-3463484 Msa0792330:intron 20.0%
!!! TCTTGCATTAATTTTTTTGA+AGG - chr5_3:3463546-3463565 None:intergenic 20.0%
!! GTAAAGTATATGATTTGAAA+AGG - chr5_3:3463739-3463758 None:intergenic 20.0%
!!! TAAGCAAATTTGACTTTTTT+TGG - chr5_3:3463837-3463856 None:intergenic 20.0%
!!! AGTCAAATTTGCTTATAATT+AGG + chr5_3:3463842-3463861 Msa0792330:intron 20.0%
!!! GTCAAATTTGCTTATAATTA+GGG + chr5_3:3463843-3463862 Msa0792330:intron 20.0%
!! GACAAAAAAGATTAAGTATA+CGG - chr5_3:3463929-3463948 None:intergenic 20.0%
!!! TATACTTAATCTTTTTTGTC+AGG + chr5_3:3463929-3463948 Msa0792330:intron 20.0%
!!! GATGTTGTATTTTAGAAAAA+TGG + chr5_3:3464144-3464163 Msa0792330:intron 20.0%
!! TGAACAAATTCATATAAAAG+TGG + chr5_3:3464487-3464506 Msa0792330:intron 20.0%
!! ATTACATTTCCAAATAAATG+AGG + chr5_3:3464707-3464726 Msa0792330:intron 20.0%
!!! ACATTGATATTGATATTTTC+TGG + chr5_3:3464804-3464823 Msa0792330:intron 20.0%
! CAGGAAAGATTAAGTAAAAA+AGG - chr5_3:3462458-3462477 None:intergenic 25.0%
!!! TAGAATTGTTTTGCTATCAT+TGG - chr5_3:3462607-3462626 None:intergenic 25.0%
! AAGGAGAATTTGAAAAATTG+AGG - chr5_3:3462734-3462753 None:intergenic 25.0%
! CTAACCAAAAAACTAATTTC+TGG - chr5_3:3463209-3463228 None:intergenic 25.0%
! GGAATCACTAGAAAAATATT+TGG + chr5_3:3463368-3463387 Msa0792330:intron 25.0%
! CTAGAAAAATATTTGGTCAA+TGG + chr5_3:3463375-3463394 Msa0792330:intron 25.0%
!!! GATTTATTCCTATTGACTTA+AGG + chr5_3:3463446-3463465 Msa0792330:intron 25.0%
!!! TTTGCATATTGGCAATTTTT+TGG + chr5_3:3463476-3463495 Msa0792330:intron 25.0%
! TGGTTCAATAATAAATGACA+CGG - chr5_3:3463792-3463811 None:intergenic 25.0%
! CATTCAATTATTGATGTATG+TGG - chr5_3:3463812-3463831 None:intergenic 25.0%
!! AATTTGCTTATAATTAGGGA+CGG + chr5_3:3463847-3463866 Msa0792330:intron 25.0%
! ACAATGCATTATTATATGCA+GGG - chr5_3:3464020-3464039 None:intergenic 25.0%
!! CTTTTCATTTTCAATCTAAC+AGG - chr5_3:3464364-3464383 None:intergenic 25.0%
! TGAACATAACAAAATTCTAC+TGG + chr5_3:3464592-3464611 Msa0792330:intron 25.0%
!! TGGACATTTTTACATACTTT+TGG - chr5_3:3464646-3464665 None:intergenic 25.0%
! ATAAAAAAGGGAGGATATAT+GGG - chr5_3:3464689-3464708 None:intergenic 25.0%
! AATAAAAAAGGGAGGATATA+TGG - chr5_3:3464690-3464709 None:intergenic 25.0%
! CAAATAAGACCTCATTTATT+TGG - chr5_3:3464719-3464738 None:intergenic 25.0%
!! TCTGATCATTGTTATTCTAA+TGG + chr5_3:3464901-3464920 Msa0792330:CDS 25.0%
TGTTGCCACATAAAGAAAAA+GGG - chr5_3:3462503-3462522 None:intergenic 30.0%
TTGTTGCCACATAAAGAAAA+AGG - chr5_3:3462504-3462523 None:intergenic 30.0%
TATTGCTCACCTAATTGAAA+GGG - chr5_3:3462546-3462565 None:intergenic 30.0%
ATATTGCTCACCTAATTGAA+AGG - chr5_3:3462547-3462566 None:intergenic 30.0%
!! GTACAATTTAAGTTGAAACC+AGG - chr5_3:3462585-3462604 None:intergenic 30.0%
ACATAGAATTCTTCAGAGTA+AGG - chr5_3:3462636-3462655 None:intergenic 30.0%
CGACTAAAAGAAGCAATAAA+AGG - chr5_3:3462753-3462772 None:intergenic 30.0%
GAGTTACTAAGACAATCAAT+GGG - chr5_3:3462828-3462847 None:intergenic 30.0%
AGAGTTACTAAGACAATCAA+TGG - chr5_3:3462829-3462848 None:intergenic 30.0%
TTAATTGAAGATCTGCACTA+AGG - chr5_3:3462977-3462996 None:intergenic 30.0%
TGCAGATCTTCAATTAACAT+GGG + chr5_3:3462980-3462999 Msa0792330:CDS 30.0%
AATTGCATACTGAAGAACAT+AGG - chr5_3:3463122-3463141 None:intergenic 30.0%
! ATGTTTAGTCAACTGATTTC+TGG - chr5_3:3463271-3463290 None:intergenic 30.0%
ATCAGTTGACTAAACATGTT+TGG + chr5_3:3463274-3463293 Msa0792330:intron 30.0%
! TTGGTAAACATTTTGCAAGT+TGG + chr5_3:3463495-3463514 Msa0792330:intron 30.0%
AACGTTGCACTCTTTAAATT+TGG - chr5_3:3463605-3463624 None:intergenic 30.0%
! AAATTTAAAGAGTGCAACGT+TGG + chr5_3:3463604-3463623 Msa0792330:intron 30.0%
CACCTTATAAGGTCAATAAA+TGG + chr5_3:3463970-3463989 Msa0792330:intron 30.0%
CTCCATTTATTGACCTTATA+AGG - chr5_3:3463975-3463994 None:intergenic 30.0%
CAATGCATTATTATATGCAG+GGG - chr5_3:3464019-3464038 None:intergenic 30.0%
GACAATGCATTATTATATGC+AGG - chr5_3:3464021-3464040 None:intergenic 30.0%
ATCAAGTTCTTGATTTACTC+CGG - chr5_3:3464127-3464146 None:intergenic 30.0%
GAAATTATTTGTGAGCAATG+AGG + chr5_3:3464283-3464302 Msa0792330:intron 30.0%
!!! TGCTTTTTTGGCTATAACAA+AGG - chr5_3:3464411-3464430 None:intergenic 30.0%
GGAAATGTAATAAAAAAGGG+AGG - chr5_3:3464698-3464717 None:intergenic 30.0%
CCTTTATTAGTGTGGTTATA+GGG - chr5_3:3464752-3464771 None:intergenic 30.0%
TCCTTTATTAGTGTGGTTAT+AGG - chr5_3:3464753-3464772 None:intergenic 30.0%
!!! ATTTTCTGGCTTCAATTTTC+AGG + chr5_3:3464818-3464837 Msa0792330:intron 30.0%
TTTCTCATACGATTCAATTG+TGG - chr5_3:3465047-3465066 None:intergenic 30.0%
TCGTATGAGAAAGTAATTCT+TGG + chr5_3:3465055-3465074 Msa0792330:CDS 30.0%
CGTATGAGAAAGTAATTCTT+GGG + chr5_3:3465056-3465075 Msa0792330:CDS 30.0%
GTATGAGAAAGTAATTCTTG+GGG + chr5_3:3465057-3465076 Msa0792330:CDS 30.0%
AGAAAAAGGGCAATCGTTTA+AGG - chr5_3:3462490-3462509 None:intergenic 35.0%
! GATTGCCCTTTTTCTTTATG+TGG + chr5_3:3462495-3462514 Msa0792330:CDS 35.0%
ATTGAAAGGGGAAACGAATT+AGG - chr5_3:3462533-3462552 None:intergenic 35.0%
ATTCGTTTCCCCTTTCAATT+AGG + chr5_3:3462534-3462553 Msa0792330:CDS 35.0%
ATTGCTCACCTAATTGAAAG+GGG - chr5_3:3462545-3462564 None:intergenic 35.0%
GCTGTTCATCTCAGAATATA+GGG + chr5_3:3462790-3462809 Msa0792330:CDS 35.0%
GAATTGACAAAGCTAACTGA+AGG - chr5_3:3462873-3462892 None:intergenic 35.0%
! AGATTTTCTTGAAACCGTTC+TGG - chr5_3:3462945-3462964 None:intergenic 35.0%
GTGCAGATCTTCAATTAACA+TGG + chr5_3:3462979-3462998 Msa0792330:CDS 35.0%
ATCTAACATCTTTACCTGGT+TGG - chr5_3:3463097-3463116 None:intergenic 35.0%
TGGATTACGATATGTACGAA+TGG - chr5_3:3463585-3463604 None:intergenic 35.0%
GTGCTAGAATTCACCTTATA+AGG + chr5_3:3463959-3463978 Msa0792330:intron 35.0%
! GTGACTTATAATTTGAGACC+CGG + chr5_3:3464105-3464124 Msa0792330:intron 35.0%
TCAAGTTCTTGATTTACTCC+GGG - chr5_3:3464126-3464145 None:intergenic 35.0%
GCTATAACAAAGGAAGAGTT+GGG - chr5_3:3464401-3464420 None:intergenic 35.0%
ATTAGTGTGGTTATAGGGAA+TGG - chr5_3:3464747-3464766 None:intergenic 35.0%
CCCTATAACCACACTAATAA+AGG + chr5_3:3464749-3464768 Msa0792330:intron 35.0%
ACTAATAAAGGATGCTCTCA+AGG + chr5_3:3464761-3464780 Msa0792330:intron 35.0%
ATCATAGCATTATGCGTTAC+TGG + chr5_3:3464872-3464891 Msa0792330:CDS 35.0%
AGAATAACAATGATCAGAGC+TGG - chr5_3:3464899-3464918 None:intergenic 35.0%
GTGTGCTACAATGATAGAAT+AGG + chr5_3:3464932-3464951 Msa0792330:CDS 35.0%
TTACTAGAGGCAACATTAAG+AGG - chr5_3:3465265-3465284 None:intergenic 35.0%
GGTGAGCAATATCCTTATTG+TGG + chr5_3:3462555-3462574 Msa0792330:CDS 40.0%
TATCCTTATTGTGGCTATCC+TGG + chr5_3:3462564-3462583 Msa0792330:CDS 40.0%
AAACCAGGATAGCCACAATA+AGG - chr5_3:3462570-3462589 None:intergenic 40.0%
ACGTTTGGGAAGACAATCAT+CGG - chr5_3:3462703-3462722 None:intergenic 40.0%
TTGAGGCTTAGAAGACGTTT+GGG - chr5_3:3462717-3462736 None:intergenic 40.0%
ATTGAGGCTTAGAAGACGTT+TGG - chr5_3:3462718-3462737 None:intergenic 40.0%
GGCTGTTCATCTCAGAATAT+AGG + chr5_3:3462789-3462808 Msa0792330:CDS 40.0%
GACAAAGCTAACTGAAGGAA+TGG - chr5_3:3462868-3462887 None:intergenic 40.0%
! AGCTTTGTCAATTCTCTGTC+TGG + chr5_3:3462879-3462898 Msa0792330:CDS 40.0%
GTCTTGCAATTGGAACTGAA+AGG - chr5_3:3462923-3462942 None:intergenic 40.0%
TTCCAATTGCAAGACCAGAA+CGG + chr5_3:3462928-3462947 Msa0792330:CDS 40.0%
GATTCACAATAACTGCAGTC+AGG - chr5_3:3463011-3463030 None:intergenic 40.0%
TGTTTCTCTGATTACCAACC+AGG + chr5_3:3463080-3463099 Msa0792330:CDS 40.0%
GGACATCTAACATCTTTACC+TGG - chr5_3:3463101-3463120 None:intergenic 40.0%
CATGTTTATCTCAATCGCTG+AGG - chr5_3:3463406-3463425 None:intergenic 40.0%
CTCAGCGATTGAGATAAACA+TGG + chr5_3:3463404-3463423 Msa0792330:intron 40.0%
GTTTGGTAAGCAATTGTCTC+TGG - chr5_3:3463650-3463669 None:intergenic 40.0%
GCTTACCAAACTAACACTCT+CGG + chr5_3:3463659-3463678 Msa0792330:intron 40.0%
!! TGATACCGAGAGTGTTAGTT+TGG - chr5_3:3463667-3463686 None:intergenic 40.0%
TTGCTTATAATTAGGGACGG+AGG + chr5_3:3463850-3463869 Msa0792330:intron 40.0%
TGCTTATAATTAGGGACGGA+GGG + chr5_3:3463851-3463870 Msa0792330:intron 40.0%
! AGGTCAATAAATGGAGTGTC+TGG + chr5_3:3463979-3463998 Msa0792330:intron 40.0%
! GGTCAATAAATGGAGTGTCT+GGG + chr5_3:3463980-3463999 Msa0792330:intron 40.0%
! GTCAATAAATGGAGTGTCTG+GGG + chr5_3:3463981-3464000 Msa0792330:intron 40.0%
GGCTATAACAAAGGAAGAGT+TGG - chr5_3:3464402-3464421 None:intergenic 40.0%
AAAGGGAGGATATATGGGTT+CGG - chr5_3:3464684-3464703 None:intergenic 40.0%
!! GAGAGCATCCTTTATTAGTG+TGG - chr5_3:3464760-3464779 None:intergenic 40.0%
TGGAAACAATGCTTGCATTC+AGG - chr5_3:3465169-3465188 None:intergenic 40.0%
ACTCATCAATGCAATCAGCA+TGG - chr5_3:3465189-3465208 None:intergenic 40.0%
! ATGCTGATTGCATTGATGAG+TGG + chr5_3:3465188-3465207 Msa0792330:CDS 40.0%
CGTTTGGGAAGACAATCATC+GGG - chr5_3:3462702-3462721 None:intergenic 45.0%
AGTCGTGACTACACCTTCTT+AGG + chr5_3:3462768-3462787 Msa0792330:CDS 45.0%
CTGAGATGAACAGCCTAAGA+AGG - chr5_3:3462784-3462803 None:intergenic 45.0%
!! AACCGTTCTGGTCTTGCAAT+TGG - chr5_3:3462933-3462952 None:intergenic 45.0%
GAATCGCATCAGCTAATGTG+TGG + chr5_3:3463026-3463045 Msa0792330:CDS 45.0%
TCCTTCTAACCTCAGCATGT+GGG - chr5_3:3463351-3463370 None:intergenic 45.0%
TTCCTTCTAACCTCAGCATG+TGG - chr5_3:3463352-3463371 None:intergenic 45.0%
GCTTATAATTAGGGACGGAG+GGG + chr5_3:3463852-3463871 Msa0792330:intron 45.0%
CTTATAATTAGGGACGGAGG+GGG + chr5_3:3463853-3463872 Msa0792330:intron 45.0%
ATTATTATATGCAGGGGCCG+GGG - chr5_3:3464013-3464032 None:intergenic 45.0%
CATTATTATATGCAGGGGCC+GGG - chr5_3:3464014-3464033 None:intergenic 45.0%
GCATTATTATATGCAGGGGC+CGG - chr5_3:3464015-3464034 None:intergenic 45.0%
TACCAACTGAGCTATGCTCA+CGG + chr5_3:3464042-3464061 Msa0792330:intron 45.0%
!!! TTTTCAGGTACATCGCGACA+AGG + chr5_3:3464833-3464852 Msa0792330:intron 45.0%
!! TTACCAGAAGTGCTGCTTCT+CGG + chr5_3:3464960-3464979 Msa0792330:CDS 45.0%
!! ATTACTGTGCCTTCAGGTTG+CGG - chr5_3:3465007-3465026 None:intergenic 45.0%
ATCCAACAACATCCGTCGTT+CGG - chr5_3:3465103-3465122 None:intergenic 45.0%
CGGATGTTGTTGGATATGCT+TGG + chr5_3:3465108-3465127 Msa0792330:CDS 45.0%
CAGGAGAGTTACGACAAACA+GGG - chr5_3:3465234-3465253 None:intergenic 45.0%
ACATTAAGAGGAGAAGGAGC+AGG - chr5_3:3465253-3465272 None:intergenic 45.0%
GAGGCAACATTAAGAGGAGA+AGG - chr5_3:3465259-3465278 None:intergenic 45.0%
!! TAAAATAATTAAAATATTCT+AGG + chr5_3:3464176-3464195 Msa0792330:intron 5.0%
!! AAAATAATTAAAATATTCTA+GGG + chr5_3:3464177-3464196 Msa0792330:intron 5.0%
! TCGTTTAAGGCGTATGCGAC+AGG - chr5_3:3462477-3462496 None:intergenic 50.0%
GTGAGCATAGCTCAGTTGGT+AGG - chr5_3:3464043-3464062 None:intergenic 50.0%
ACCAACTGAGCTATGCTCAC+GGG + chr5_3:3464043-3464062 Msa0792330:intron 50.0%
TTCGGAGTTGCAGCAACTCA+TGG - chr5_3:3464666-3464685 None:intergenic 50.0%
GCAATATCACCGCAACCTGA+AGG + chr5_3:3464995-3465014 Msa0792330:CDS 50.0%
CCTGCAATTACTGTGCCTTC+AGG - chr5_3:3465013-3465032 None:intergenic 50.0%
! CCTGAAGGCACAGTAATTGC+AGG + chr5_3:3465010-3465029 Msa0792330:CDS 50.0%
GCAGGAGAGTTACGACAAAC+AGG - chr5_3:3465235-3465254 None:intergenic 50.0%
ACTAGTTGGCCCACATGCTG+AGG + chr5_3:3463339-3463358 Msa0792330:intron 55.0%
GCCCACATGCTGAGGTTAGA+AGG + chr5_3:3463347-3463366 Msa0792330:intron 55.0%
TCCCGTGAGCATAGCTCAGT+TGG - chr5_3:3464047-3464066 None:intergenic 55.0%
TGACCGAGAAGCAGCACTTC+TGG - chr5_3:3464966-3464985 None:intergenic 55.0%
GACCGAACGACGGATGTTGT+TGG + chr5_3:3465098-3465117 Msa0792330:CDS 55.0%
! AGTGTCTGGGGTTCGAACCC+CGG + chr5_3:3463993-3464012 Msa0792330:intron 60.0%
TGCTTCTCGGTCAGCACCAG+CGG + chr5_3:3464973-3464992 Msa0792330:CDS 60.0%
GGTTGCGGTGATATTGCCGC+TGG - chr5_3:3464992-3465011 None:intergenic 60.0%
CGTGTTCCTGGACCGAACGA+CGG + chr5_3:3465088-3465107 Msa0792330:CDS 60.0%
CAACATCCGTCGTTCGGTCC+AGG - chr5_3:3465097-3465116 None:intergenic 60.0%
GGGGAGAGTCGACGTGTTCC+TGG + chr5_3:3465076-3465095 Msa0792330:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 3462438 3465291 3462438 ID=Msa0792330;Name=Msa0792330
chr5_3 mRNA 3462438 3465291 3462438 ID=Msa0792330-mRNA-1;Parent=Msa0792330;Name=Msa0792330-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|371
chr5_3 exon 3462438 3463101 3462438 ID=Msa0792330-mRNA-1:exon:1502;Parent=Msa0792330-mRNA-1
chr5_3 exon 3464840 3465291 3464840 ID=Msa0792330-mRNA-1:exon:1503;Parent=Msa0792330-mRNA-1
chr5_3 CDS 3462438 3463101 3462438 ID=Msa0792330-mRNA-1:cds;Parent=Msa0792330-mRNA-1
chr5_3 CDS 3464840 3465291 3464840 ID=Msa0792330-mRNA-1:cds;Parent=Msa0792330-mRNA-1
Gene Sequence

>Msa0792330

ATGGACATCTTGAAAGTCCTTTTTTACTTAATCTTTCCTGTCGCATACGCCTTAAACGATTGCCCTTTTTCTTTATGTGGCAACAATAGTTTCCTAATTCGTTTCCCCTTTCAATTAGGTGAGCAATATCCTTATTGTGGCTATCCTGGTTTCAACTTAAATTGTACCAATGATAGCAAAACAATTCTAAAACTTCCTTACTCTGAAGAATTCTATGTACGCAGCATAAACTATCTCACGCAACAGATACTACTCTATGATCCCGATGATTGTCTTCCCAAACGTCTTCTAAGCCTCAATTTTTCAAATTCTCCTTTTATTGCTTCTTTTAGTCGTGACTACACCTTCTTAGGCTGTTCATCTCAGAATATAGGGTCACAATTCATTCCCATTGATTGTCTTAGTAACTCTACATATTTTGTATCAGCCATTCCTTCAGTTAGCTTTGTCAATTCTCTGTCTGGTTGCTCTGTTATAAAAAACCTTTCAGTTCCAATTGCAAGACCAGAACGGTTTCAAGAAAATCTAAGAGATGACCTTAGTGCAGATCTTCAATTAACATGGGATAAACCTGACTGCAGTTATTGTGAATCGCATCAGCTAATGTGTGGATTTGAAAGCATTAACAGCAATCAAGTTGTCTGTTTCTCTGATTACCAACCAGGTACATCGCGACAAGGACTTAAAATTTTCAGAATCATAGCATTATGCGTTACTGGACCAGCTCTGATCATTGTTATTCTAATGGCAAGTTGTGTGTGCTACAATGATAGAATAGGCAACATTACCAGAAGTGCTGCTTCTCGGTCAGCACCAGCGGCAATATCACCGCAACCTGAAGGCACAGTAATTGCAGGTTTAGATGAATCCACAATTGAATCGTATGAGAAAGTAATTCTTGGGGAGAGTCGACGTGTTCCTGGACCGAACGACGGATGTTGTTGGATATGCTTGGCAGAATACAACAGTAAAGAGACAGTAAGATGCATTCCTGAATGCAAGCATTGTTTCCATGCTGATTGCATTGATGAGTGGCTTCGTATGAATGTTACATGCCCTGTTTGTCGTAACTCTCCTGCTCCTTCTCCTCTTAATGTTGCCTCTAGTAATGTTTGA
Protein sequence

>Msa0792330

MDILKVLFYLIFPVAYALNDCPFSLCGNNSFLIRFPFQLGEQYPYCGYPGFNLNCTNDSKTILKLPYSEEFYVRSINYLTQQILLYDPDDCLPKRLLSLNFSNSPFIASFSRDYTFLGCSSQNIGSQFIPIDCLSNSTYFVSAIPSVSFVNSLSGCSVIKNLSVPIARPERFQENLRDDLSADLQLTWDKPDCSYCESHQLMCGFESINSNQVVCFSDYQPGTSRQGLKIFRIIALCVTGPALIIVILMASCVCYNDRIGNITRSAASRSAPAAISPQPEGTVIAGLDESTIESYEKVILGESRRVPGPNDGCCWICLAEYNSKETVRCIPECKHCFHADCIDEWLRMNVTCPVCRNSPAPSPLNVASSNV*