AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1457240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 60 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGATCGAGGATTGGTAATT+TGG 0.177882 tig0038559:+24684 None:intergenic
TGTCTTTCAAACTCTATTCT+AGG 0.184570 tig0038559:+24001 None:intergenic
AAATGAACAGTTATGGTTTA+TGG 0.197069 tig0038559:-23757 Msa1457240:three_prime_UTR
CAAATTACAAATACTTCATT+TGG 0.287255 tig0038559:-24063 Msa1457240:three_prime_UTR
ATGACAGATATAAAGCTTCT+AGG 0.322425 tig0038559:+24750 None:intergenic
TGTTATCTGCTCATTCAATA+AGG 0.344676 tig0038559:-24188 Msa1457240:intron
ACAGAGAAGTAGAGTTATGA+TGG 0.345223 tig0038559:-24362 Msa1457240:intron
AAATACTTCATTTGGAAAGA+TGG 0.382527 tig0038559:-24055 Msa1457240:three_prime_UTR
CACCTCCATGGACTTTGAAA+TGG 0.389456 tig0038559:+24328 None:intergenic
TAGTGTTTATTTGTATTCAT+AGG 0.408613 tig0038559:-23849 Msa1457240:three_prime_UTR
TCGAAGTAAATGAACAGTTA+TGG 0.408932 tig0038559:-23764 Msa1457240:three_prime_UTR
TGAAGAGTGTCACATGTAAA+GGG 0.427701 tig0038559:-24291 Msa1457240:three_prime_UTR
TGAAGTATTTGTAATTTGCC+TGG 0.440764 tig0038559:+24068 None:intergenic
TTCGTATACGCACAGATCAT+TGG 0.443102 tig0038559:-24799 Msa1457240:five_prime_UTR
TCGTAGGTCATGTTGTTTGT+TGG 0.448334 tig0038559:-23815 Msa1457240:three_prime_UTR
AAAGATCACCAACTTCCATC+TGG 0.451508 tig0038559:+24831 None:intergenic
AATAATGAAGAGTATGAAAA+GGG 0.464170 tig0038559:-24935 Msa1457240:five_prime_UTR
CACAGATCATTGGCCTAGCT+TGG 0.466019 tig0038559:-24789 Msa1457240:five_prime_UTR
GTTTGAAAGACAATTTGTAA+TGG 0.471333 tig0038559:-23990 Msa1457240:three_prime_UTR
CAATAATGAAGAGTATGAAA+AGG 0.476333 tig0038559:-24936 Msa1457240:five_prime_UTR
TCCCAGGTTTAATCGTGTGT+TGG 0.481497 tig0038559:+24215 None:intergenic
CAGAGAAGTAGAGTTATGAT+GGG 0.481922 tig0038559:-24361 Msa1457240:CDS
TGAAAGACAATTTGTAATGG+AGG 0.485109 tig0038559:-23987 Msa1457240:three_prime_UTR
GATCTGTGCGTATACGAACT+AGG 0.488180 tig0038559:+24804 None:intergenic
GTGAAGAGTGTCACATGTAA+AGG 0.488306 tig0038559:-24292 Msa1457240:three_prime_UTR
CACCAACTTCCATCTGGAAA+AGG 0.495097 tig0038559:+24837 None:intergenic
GCAAAGCCACAATCGACATA+TGG 0.499824 tig0038559:+24608 None:intergenic
AGTAGAGTTATGATGGGAGG+TGG 0.517962 tig0038559:-24355 Msa1457240:CDS
AGCCAACACACGATTAAACC+TGG 0.536131 tig0038559:-24217 Msa1457240:three_prime_UTR
AGGGAGGTATTATCAATAGC+AGG 0.536649 tig0038559:-24916 Msa1457240:five_prime_UTR
CTTCCATCTGGAAAAGGAGA+TGG 0.541956 tig0038559:+24843 None:intergenic
ATCACAAGCTGATCGAGGAT+TGG 0.543704 tig0038559:+24676 None:intergenic
ACTTCATTTGGAAAGATGGT+GGG 0.550297 tig0038559:-24051 Msa1457240:three_prime_UTR
TCATCAATGTAGGCCAAGCT+AGG 0.555240 tig0038559:+24776 None:intergenic
AAAGGCTCTCTTCACCTCCA+TGG 0.555290 tig0038559:+24316 None:intergenic
ATCTGTGCGTATACGAACTA+GGG 0.556185 tig0038559:+24805 None:intergenic
TACTTCATTTGGAAAGATGG+TGG 0.557698 tig0038559:-24052 Msa1457240:three_prime_UTR
GTACACCATTTCAAAGTCCA+TGG 0.558332 tig0038559:-24333 Msa1457240:CDS
GGAGATGGAACCGCGGTGGA+AGG 0.564698 tig0038559:+24858 None:intergenic
GAGCAGATAACAGTCTTCCC+AGG 0.575774 tig0038559:+24199 None:intergenic
CTTCATTATTGTATGTAAGA+CGG 0.577163 tig0038559:+24948 None:intergenic
AGAGAATGCAATGGATGATG+TGG 0.581557 tig0038559:-23950 Msa1457240:three_prime_UTR
CTTTCATCACAGTTGCAACA+AGG 0.601002 tig0038559:+24255 None:intergenic
TTCTAGGTAATCATCAATGT+AGG 0.605445 tig0038559:+24766 None:intergenic
CATGTGACACTCTTCACGAA+AGG 0.615991 tig0038559:+24298 None:intergenic
AGAAGTAGAGTTATGATGGG+AGG 0.619251 tig0038559:-24358 Msa1457240:CDS
ATAGGACATAGTGCATTCGT+AGG 0.632440 tig0038559:-23831 Msa1457240:three_prime_UTR
AATGAAGAGTATGAAAAGGG+AGG 0.635041 tig0038559:-24932 Msa1457240:five_prime_UTR
GCCAACACACGATTAAACCT+GGG 0.646274 tig0038559:-24216 Msa1457240:three_prime_UTR
TATGTGCCATATGTCGATTG+TGG 0.649512 tig0038559:-24614 Msa1457240:CDS
TTATTGTATGTAAGACGGAG+TGG 0.654217 tig0038559:+24953 None:intergenic
GAAGAGTGTCACATGTAAAG+GGG 0.661434 tig0038559:-24290 Msa1457240:three_prime_UTR
CTTGTTGCAACTGTGATGAA+AGG 0.663166 tig0038559:-24254 Msa1457240:three_prime_UTR
ACATCATCACAAGCTGATCG+AGG 0.688565 tig0038559:+24671 None:intergenic
TTCTAATATTCCTTCCACCG+CGG 0.694161 tig0038559:-24868 Msa1457240:five_prime_UTR
AAAAGGAGATGGAACCGCGG+TGG 0.695999 tig0038559:+24854 None:intergenic
GGTGTGAGTACTTTATGTAG+CGG 0.706839 tig0038559:-23794 Msa1457240:three_prime_UTR
ACTTTGAAGAGAGAATGCAA+TGG 0.726106 tig0038559:-23959 Msa1457240:three_prime_UTR
CACCATTTCAAAGTCCATGG+AGG 0.730620 tig0038559:-24330 Msa1457240:CDS
TGGAAAAGGAGATGGAACCG+CGG 0.748922 tig0038559:+24851 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATAGTTATTTCAATTTAA+AGG + tig0038559:24293-24312 None:intergenic 10.0%
!!! TTATAGTTATTTCAATTTAA+AGG + tig0038559:24293-24312 None:intergenic 10.0%
!!! AATAATGAAGAGTATGAAAA+GGG - tig0038559:23774-23793 Msa1457240:three_prime_UTR 20.0%
!!! TTTAGTTCTTGAAAATGATA+TGG - tig0038559:24218-24237 Msa1457240:three_prime_UTR 20.0%
!! ATTTCAATTTAAAGGACATT+GGG + tig0038559:24285-24304 None:intergenic 20.0%
!! TATTTCAATTTAAAGGACAT+TGG + tig0038559:24286-24305 None:intergenic 20.0%
!! CAAATTACAAATACTTCATT+TGG - tig0038559:24646-24665 Msa1457240:CDS 20.0%
!! TAGTGTTTATTTGTATTCAT+AGG - tig0038559:24860-24879 Msa1457240:five_prime_UTR 20.0%
!!! AATAATGAAGAGTATGAAAA+GGG - tig0038559:23774-23793 Msa1457240:three_prime_UTR 20.0%
!!! TTTAGTTCTTGAAAATGATA+TGG - tig0038559:24218-24237 Msa1457240:three_prime_UTR 20.0%
!! ATTTCAATTTAAAGGACATT+GGG + tig0038559:24285-24304 None:intergenic 20.0%
!! TATTTCAATTTAAAGGACAT+TGG + tig0038559:24286-24305 None:intergenic 20.0%
!! CAAATTACAAATACTTCATT+TGG - tig0038559:24646-24665 Msa1457240:CDS 20.0%
!! TAGTGTTTATTTGTATTCAT+AGG - tig0038559:24860-24879 Msa1457240:five_prime_UTR 20.0%
! CTTCATTATTGTATGTAAGA+CGG + tig0038559:23764-23783 None:intergenic 25.0%
!! CAATAATGAAGAGTATGAAA+AGG - tig0038559:23773-23792 Msa1457240:three_prime_UTR 25.0%
! TTCTTGAAAATGATATGGTT+AGG - tig0038559:24223-24242 Msa1457240:three_prime_UTR 25.0%
!!! GTTTTAATTGATTGTCACTT+TGG + tig0038559:24250-24269 None:intergenic 25.0%
! TCAATAAGGTTTGTAAATGT+TGG - tig0038559:24535-24554 Msa1457240:intron 25.0%
!!! CTATATTTTGATGTTTTTGC+AGG - tig0038559:24614-24633 Msa1457240:CDS 25.0%
! AAATACTTCATTTGGAAAGA+TGG - tig0038559:24654-24673 Msa1457240:CDS 25.0%
! GTTTGAAAGACAATTTGTAA+TGG - tig0038559:24719-24738 Msa1457240:five_prime_UTR 25.0%
! AAATGAACAGTTATGGTTTA+TGG - tig0038559:24952-24971 Msa1457240:five_prime_UTR 25.0%
! CTTCATTATTGTATGTAAGA+CGG + tig0038559:23764-23783 None:intergenic 25.0%
!! CAATAATGAAGAGTATGAAA+AGG - tig0038559:23773-23792 Msa1457240:three_prime_UTR 25.0%
! TTCTTGAAAATGATATGGTT+AGG - tig0038559:24223-24242 Msa1457240:three_prime_UTR 25.0%
!!! GTTTTAATTGATTGTCACTT+TGG + tig0038559:24250-24269 None:intergenic 25.0%
! TCAATAAGGTTTGTAAATGT+TGG - tig0038559:24535-24554 Msa1457240:intron 25.0%
!!! CTATATTTTGATGTTTTTGC+AGG - tig0038559:24614-24633 Msa1457240:CDS 25.0%
! AAATACTTCATTTGGAAAGA+TGG - tig0038559:24654-24673 Msa1457240:CDS 25.0%
! GTTTGAAAGACAATTTGTAA+TGG - tig0038559:24719-24738 Msa1457240:five_prime_UTR 25.0%
! AAATGAACAGTTATGGTTTA+TGG - tig0038559:24952-24971 Msa1457240:five_prime_UTR 25.0%
AATAGCAGGCAAAAAAATCT+GGG - tig0038559:23807-23826 Msa1457240:three_prime_UTR 30.0%
TTCTAGGTAATCATCAATGT+AGG + tig0038559:23946-23965 None:intergenic 30.0%
ATGACAGATATAAAGCTTCT+AGG + tig0038559:23962-23981 None:intergenic 30.0%
TGTTATCTGCTCATTCAATA+AGG - tig0038559:24521-24540 Msa1457240:intron 30.0%
ATATAGATTCCTCAACAACA+TGG + tig0038559:24600-24619 None:intergenic 30.0%
! TGAAGTATTTGTAATTTGCC+TGG + tig0038559:24644-24663 None:intergenic 30.0%
TGTCTTTCAAACTCTATTCT+AGG + tig0038559:24711-24730 None:intergenic 30.0%
TGAAAGACAATTTGTAATGG+AGG - tig0038559:24722-24741 Msa1457240:five_prime_UTR 30.0%
TCGAAGTAAATGAACAGTTA+TGG - tig0038559:24945-24964 Msa1457240:five_prime_UTR 30.0%
AATAGCAGGCAAAAAAATCT+GGG - tig0038559:23807-23826 Msa1457240:three_prime_UTR 30.0%
TTCTAGGTAATCATCAATGT+AGG + tig0038559:23946-23965 None:intergenic 30.0%
ATGACAGATATAAAGCTTCT+AGG + tig0038559:23962-23981 None:intergenic 30.0%
TGTTATCTGCTCATTCAATA+AGG - tig0038559:24521-24540 Msa1457240:intron 30.0%
ATATAGATTCCTCAACAACA+TGG + tig0038559:24600-24619 None:intergenic 30.0%
! TGAAGTATTTGTAATTTGCC+TGG + tig0038559:24644-24663 None:intergenic 30.0%
TGTCTTTCAAACTCTATTCT+AGG + tig0038559:24711-24730 None:intergenic 30.0%
TGAAAGACAATTTGTAATGG+AGG - tig0038559:24722-24741 Msa1457240:five_prime_UTR 30.0%
TCGAAGTAAATGAACAGTTA+TGG - tig0038559:24945-24964 Msa1457240:five_prime_UTR 30.0%
TTATTGTATGTAAGACGGAG+TGG + tig0038559:23759-23778 None:intergenic 35.0%
! AATGAAGAGTATGAAAAGGG+AGG - tig0038559:23777-23796 Msa1457240:three_prime_UTR 35.0%
CAATAGCAGGCAAAAAAATC+TGG - tig0038559:23806-23825 Msa1457240:three_prime_UTR 35.0%
! ATTTTTGCTCGTGCAATTTG+AGG - tig0038559:24140-24159 Msa1457240:intron 35.0%
CAAGAACTAAAACTGCATGA+AGG + tig0038559:24209-24228 None:intergenic 35.0%
ACAGAGAAGTAGAGTTATGA+TGG - tig0038559:24347-24366 Msa1457240:CDS 35.0%
CAGAGAAGTAGAGTTATGAT+GGG - tig0038559:24348-24367 Msa1457240:CDS 35.0%
!! TGAAGAGTGTCACATGTAAA+GGG - tig0038559:24418-24437 Msa1457240:intron 35.0%
TTCAGACATGTACGAAGAAA+TGG + tig0038559:24575-24594 None:intergenic 35.0%
TACTTCATTTGGAAAGATGG+TGG - tig0038559:24657-24676 Msa1457240:CDS 35.0%
ACTTCATTTGGAAAGATGGT+GGG - tig0038559:24658-24677 Msa1457240:CDS 35.0%
! ACTTTGAAGAGAGAATGCAA+TGG - tig0038559:24750-24769 Msa1457240:five_prime_UTR 35.0%
TTATTGTATGTAAGACGGAG+TGG + tig0038559:23759-23778 None:intergenic 35.0%
! AATGAAGAGTATGAAAAGGG+AGG - tig0038559:23777-23796 Msa1457240:three_prime_UTR 35.0%
CAATAGCAGGCAAAAAAATC+TGG - tig0038559:23806-23825 Msa1457240:three_prime_UTR 35.0%
! ATTTTTGCTCGTGCAATTTG+AGG - tig0038559:24140-24159 Msa1457240:intron 35.0%
CAAGAACTAAAACTGCATGA+AGG + tig0038559:24209-24228 None:intergenic 35.0%
ACAGAGAAGTAGAGTTATGA+TGG - tig0038559:24347-24366 Msa1457240:CDS 35.0%
CAGAGAAGTAGAGTTATGAT+GGG - tig0038559:24348-24367 Msa1457240:CDS 35.0%
!! TGAAGAGTGTCACATGTAAA+GGG - tig0038559:24418-24437 Msa1457240:intron 35.0%
TTCAGACATGTACGAAGAAA+TGG + tig0038559:24575-24594 None:intergenic 35.0%
TACTTCATTTGGAAAGATGG+TGG - tig0038559:24657-24676 Msa1457240:CDS 35.0%
ACTTCATTTGGAAAGATGGT+GGG - tig0038559:24658-24677 Msa1457240:CDS 35.0%
! ACTTTGAAGAGAGAATGCAA+TGG - tig0038559:24750-24769 Msa1457240:five_prime_UTR 35.0%
!! AGGGAGGTATTATCAATAGC+AGG - tig0038559:23793-23812 Msa1457240:three_prime_UTR 40.0%
TTCTAATATTCCTTCCACCG+CGG - tig0038559:23841-23860 Msa1457240:three_prime_UTR 40.0%
AAAGATCACCAACTTCCATC+TGG + tig0038559:23881-23900 None:intergenic 40.0%
ATCTGTGCGTATACGAACTA+GGG + tig0038559:23907-23926 None:intergenic 40.0%
TTCGTATACGCACAGATCAT+TGG - tig0038559:23910-23929 Msa1457240:three_prime_UTR 40.0%
! CTGATCGAGGATTGGTAATT+TGG + tig0038559:24028-24047 None:intergenic 40.0%
! TATGTGCCATATGTCGATTG+TGG - tig0038559:24095-24114 Msa1457240:intron 40.0%
! AGAAGTAGAGTTATGATGGG+AGG - tig0038559:24351-24370 Msa1457240:CDS 40.0%
GTACACCATTTCAAAGTCCA+TGG - tig0038559:24376-24395 Msa1457240:intron 40.0%
!! GTGAAGAGTGTCACATGTAA+AGG - tig0038559:24417-24436 Msa1457240:intron 40.0%
!! GAAGAGTGTCACATGTAAAG+GGG - tig0038559:24419-24438 Msa1457240:intron 40.0%
CTTTCATCACAGTTGCAACA+AGG + tig0038559:24457-24476 None:intergenic 40.0%
CTTGTTGCAACTGTGATGAA+AGG - tig0038559:24455-24474 Msa1457240:intron 40.0%
GTCTGAATTCCATGTTGTTG+AGG - tig0038559:24588-24607 Msa1457240:CDS 40.0%
AGAGAATGCAATGGATGATG+TGG - tig0038559:24759-24778 Msa1457240:five_prime_UTR 40.0%
ATAGGACATAGTGCATTCGT+AGG - tig0038559:24878-24897 Msa1457240:five_prime_UTR 40.0%
! TCGTAGGTCATGTTGTTTGT+TGG - tig0038559:24894-24913 Msa1457240:five_prime_UTR 40.0%
GGTGTGAGTACTTTATGTAG+CGG - tig0038559:24915-24934 Msa1457240:five_prime_UTR 40.0%
!! AGGGAGGTATTATCAATAGC+AGG - tig0038559:23793-23812 Msa1457240:three_prime_UTR 40.0%
TTCTAATATTCCTTCCACCG+CGG - tig0038559:23841-23860 Msa1457240:three_prime_UTR 40.0%
AAAGATCACCAACTTCCATC+TGG + tig0038559:23881-23900 None:intergenic 40.0%
ATCTGTGCGTATACGAACTA+GGG + tig0038559:23907-23926 None:intergenic 40.0%
TTCGTATACGCACAGATCAT+TGG - tig0038559:23910-23929 Msa1457240:three_prime_UTR 40.0%
! CTGATCGAGGATTGGTAATT+TGG + tig0038559:24028-24047 None:intergenic 40.0%
! TATGTGCCATATGTCGATTG+TGG - tig0038559:24095-24114 Msa1457240:intron 40.0%
! AGAAGTAGAGTTATGATGGG+AGG - tig0038559:24351-24370 Msa1457240:CDS 40.0%
GTACACCATTTCAAAGTCCA+TGG - tig0038559:24376-24395 Msa1457240:intron 40.0%
!! GTGAAGAGTGTCACATGTAA+AGG - tig0038559:24417-24436 Msa1457240:intron 40.0%
!! GAAGAGTGTCACATGTAAAG+GGG - tig0038559:24419-24438 Msa1457240:intron 40.0%
CTTTCATCACAGTTGCAACA+AGG + tig0038559:24457-24476 None:intergenic 40.0%
CTTGTTGCAACTGTGATGAA+AGG - tig0038559:24455-24474 Msa1457240:intron 40.0%
GTCTGAATTCCATGTTGTTG+AGG - tig0038559:24588-24607 Msa1457240:CDS 40.0%
AGAGAATGCAATGGATGATG+TGG - tig0038559:24759-24778 Msa1457240:five_prime_UTR 40.0%
ATAGGACATAGTGCATTCGT+AGG - tig0038559:24878-24897 Msa1457240:five_prime_UTR 40.0%
! TCGTAGGTCATGTTGTTTGT+TGG - tig0038559:24894-24913 Msa1457240:five_prime_UTR 40.0%
GGTGTGAGTACTTTATGTAG+CGG - tig0038559:24915-24934 Msa1457240:five_prime_UTR 40.0%
! GTTCCATCTCCTTTTCCAGA+TGG - tig0038559:23863-23882 Msa1457240:three_prime_UTR 45.0%
CTTCCATCTGGAAAAGGAGA+TGG + tig0038559:23869-23888 None:intergenic 45.0%
! CTCCTTTTCCAGATGGAAGT+TGG - tig0038559:23870-23889 Msa1457240:three_prime_UTR 45.0%
CACCAACTTCCATCTGGAAA+AGG + tig0038559:23875-23894 None:intergenic 45.0%
GATCTGTGCGTATACGAACT+AGG + tig0038559:23908-23927 None:intergenic 45.0%
TCATCAATGTAGGCCAAGCT+AGG + tig0038559:23936-23955 None:intergenic 45.0%
ATCACAAGCTGATCGAGGAT+TGG + tig0038559:24036-24055 None:intergenic 45.0%
ACATCATCACAAGCTGATCG+AGG + tig0038559:24041-24060 None:intergenic 45.0%
GCAAAGCCACAATCGACATA+TGG + tig0038559:24104-24123 None:intergenic 45.0%
! AGTAGAGTTATGATGGGAGG+TGG - tig0038559:24354-24373 Msa1457240:CDS 45.0%
CACCATTTCAAAGTCCATGG+AGG - tig0038559:24379-24398 Msa1457240:intron 45.0%
! CACCTCCATGGACTTTGAAA+TGG + tig0038559:24384-24403 None:intergenic 45.0%
CATGTGACACTCTTCACGAA+AGG + tig0038559:24414-24433 None:intergenic 45.0%
AGCCAACACACGATTAAACC+TGG - tig0038559:24492-24511 Msa1457240:intron 45.0%
GCCAACACACGATTAAACCT+GGG - tig0038559:24493-24512 Msa1457240:intron 45.0%
TCCCAGGTTTAATCGTGTGT+TGG + tig0038559:24497-24516 None:intergenic 45.0%
!! GATGTTTTTGCAGGATGACC+AGG - tig0038559:24623-24642 Msa1457240:CDS 45.0%
! GTTCCATCTCCTTTTCCAGA+TGG - tig0038559:23863-23882 Msa1457240:three_prime_UTR 45.0%
CTTCCATCTGGAAAAGGAGA+TGG + tig0038559:23869-23888 None:intergenic 45.0%
! CTCCTTTTCCAGATGGAAGT+TGG - tig0038559:23870-23889 Msa1457240:three_prime_UTR 45.0%
CACCAACTTCCATCTGGAAA+AGG + tig0038559:23875-23894 None:intergenic 45.0%
GATCTGTGCGTATACGAACT+AGG + tig0038559:23908-23927 None:intergenic 45.0%
TCATCAATGTAGGCCAAGCT+AGG + tig0038559:23936-23955 None:intergenic 45.0%
ATCACAAGCTGATCGAGGAT+TGG + tig0038559:24036-24055 None:intergenic 45.0%
ACATCATCACAAGCTGATCG+AGG + tig0038559:24041-24060 None:intergenic 45.0%
GCAAAGCCACAATCGACATA+TGG + tig0038559:24104-24123 None:intergenic 45.0%
! AGTAGAGTTATGATGGGAGG+TGG - tig0038559:24354-24373 Msa1457240:CDS 45.0%
CACCATTTCAAAGTCCATGG+AGG - tig0038559:24379-24398 Msa1457240:intron 45.0%
! CACCTCCATGGACTTTGAAA+TGG + tig0038559:24384-24403 None:intergenic 45.0%
CATGTGACACTCTTCACGAA+AGG + tig0038559:24414-24433 None:intergenic 45.0%
AGCCAACACACGATTAAACC+TGG - tig0038559:24492-24511 Msa1457240:intron 45.0%
GCCAACACACGATTAAACCT+GGG - tig0038559:24493-24512 Msa1457240:intron 45.0%
TCCCAGGTTTAATCGTGTGT+TGG + tig0038559:24497-24516 None:intergenic 45.0%
!! GATGTTTTTGCAGGATGACC+AGG - tig0038559:24623-24642 Msa1457240:CDS 45.0%
TGGAAAAGGAGATGGAACCG+CGG + tig0038559:23861-23880 None:intergenic 50.0%
! CACAGATCATTGGCCTAGCT+TGG - tig0038559:23920-23939 Msa1457240:three_prime_UTR 50.0%
CTTGCAGCAACGGAGGAATA+AGG + tig0038559:24316-24335 None:intergenic 50.0%
CCAAACTCAGCTTGCAGCAA+CGG + tig0038559:24326-24345 None:intergenic 50.0%
AAAGGCTCTCTTCACCTCCA+TGG + tig0038559:24396-24415 None:intergenic 50.0%
GAGCAGATAACAGTCTTCCC+AGG + tig0038559:24513-24532 None:intergenic 50.0%
TGGAAAAGGAGATGGAACCG+CGG + tig0038559:23861-23880 None:intergenic 50.0%
! CACAGATCATTGGCCTAGCT+TGG - tig0038559:23920-23939 Msa1457240:three_prime_UTR 50.0%
CTTGCAGCAACGGAGGAATA+AGG + tig0038559:24316-24335 None:intergenic 50.0%
CCAAACTCAGCTTGCAGCAA+CGG + tig0038559:24326-24345 None:intergenic 50.0%
AAAGGCTCTCTTCACCTCCA+TGG + tig0038559:24396-24415 None:intergenic 50.0%
GAGCAGATAACAGTCTTCCC+AGG + tig0038559:24513-24532 None:intergenic 50.0%
AAAAGGAGATGGAACCGCGG+TGG + tig0038559:23858-23877 None:intergenic 55.0%
AACTCAGCTTGCAGCAACGG+AGG + tig0038559:24323-24342 None:intergenic 55.0%
CCGTTGCTGCAAGCTGAGTT+TGG - tig0038559:24323-24342 Msa1457240:exon 55.0%
AAAAGGAGATGGAACCGCGG+TGG + tig0038559:23858-23877 None:intergenic 55.0%
AACTCAGCTTGCAGCAACGG+AGG + tig0038559:24323-24342 None:intergenic 55.0%
CCGTTGCTGCAAGCTGAGTT+TGG - tig0038559:24323-24342 Msa1457240:exon 55.0%
GGAGATGGAACCGCGGTGGA+AGG + tig0038559:23854-23873 None:intergenic 65.0%
GGAGATGGAACCGCGGTGGA+AGG + tig0038559:23854-23873 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0038559 gene 23748 24983 23748 ID=Msa1457240;Name=Msa1457240
tig0038559 mRNA 23748 24983 23748 ID=Msa1457240-mRNA-1;Parent=Msa1457240;Name=Msa1457240-mRNA-1;_AED=0.09;_eAED=0.09;_QI=306|1|1|1|0.5|0.33|3|486|53
tig0038559 exon 23748 24095 23748 ID=Msa1457240-mRNA-1:exon:21046;Parent=Msa1457240-mRNA-1
tig0038559 exon 24189 24380 24189 ID=Msa1457240-mRNA-1:exon:21045;Parent=Msa1457240-mRNA-1
tig0038559 exon 24570 24983 24570 ID=Msa1457240-mRNA-1:exon:21044;Parent=Msa1457240-mRNA-1
tig0038559 five_prime_UTR 24678 24983 24678 ID=Msa1457240-mRNA-1:five_prime_utr;Parent=Msa1457240-mRNA-1
tig0038559 CDS 24570 24677 24570 ID=Msa1457240-mRNA-1:cds;Parent=Msa1457240-mRNA-1
tig0038559 CDS 24327 24380 24327 ID=Msa1457240-mRNA-1:cds;Parent=Msa1457240-mRNA-1
tig0038559 three_prime_UTR 24189 24326 24189 ID=Msa1457240-mRNA-1:three_prime_utr;Parent=Msa1457240-mRNA-1
tig0038559 three_prime_UTR 23748 24095 23748 ID=Msa1457240-mRNA-1:three_prime_utr;Parent=Msa1457240-mRNA-1
Gene Sequence

>Msa1457240

ATGATGTATGTCTTTTTTATTCTTTTGCAATGTACAGTTTGTATGTGCCATATGTCGATTGTGGCTTTGCTGTTCAATAAGAACACATTTTTGCTCGTGCAATTTGAGAGAAGTAGAGTTATGATGGGAGGTGGTACACCATTTCAAAGTCCATGGAGGTGA
Protein sequence

>Msa1457240

MMYVFFILLQCTVCMCHMSIVALLFNKNTFLLVQFERSRVMMGGGTPFQSPWR*