AlfalfaGEDB | MS.gene057783

M. sativa cultivar XinJiangDaYe / MS.gene057783

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057783.t1	AES89350.1	96.6	87	2	1	1	87	1	86	5.40E-39	169.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057783.t1	P33080	72.2	90	19	2	1	85	1	89	1.7e-30	132.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057783.t1	G7JMA7	96.6	87	2	1	1	87	1	86	3.9e-39	169.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057783	MS.gene99375	0.802299	5.99E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene062943	MS.gene057783	PPI
MS.gene01358	MS.gene057783	PPI
MS.gene057783	MS.gene75314	PPI
MS.gene057783	MS.gene01764	PPI
MS.gene003608	MS.gene057783	PPI
MS.gene057783	MS.gene75679	PPI
MS.gene40326	MS.gene057783	PPI
MS.gene057783	MS.gene40325	PPI
MS.gene062952	MS.gene057783	PPI
MS.gene057783	MS.gene68760	PPI
MS.gene057783	MS.gene031198	PPI
MS.gene50676	MS.gene057783	PPI
MS.gene23370	MS.gene057783	PPI
MS.gene50766	MS.gene057783	PPI
MS.gene057783	MS.gene057797	PPI
MS.gene003594	MS.gene057783	PPI
MS.gene057783	MS.gene003614	PPI
MS.gene057783	MS.gene77528	PPI
MS.gene50665	MS.gene057783	PPI
MS.gene057783	MS.gene79886	PPI
MS.gene057783	MS.gene014671	PPI
MS.gene057783	MS.gene003596	PPI
MS.gene057783	MS.gene062988	PPI
MS.gene057783	MS.gene23371	PPI
MS.gene057783	MS.gene75672	PPI
MS.gene057783	MS.gene75674	PPI
MS.gene057783	MS.gene50654	PPI
MS.gene057783	MS.gene062974	PPI
MS.gene057783	MS.gene003583	PPI
MS.gene057783	MS.gene062951	PPI
MS.gene057783	MS.gene057801	PPI
MS.gene057783	MS.gene77527	PPI
MS.gene50763	MS.gene057783	PPI
MS.gene057783	MS.gene77524	PPI
MS.gene057783	MS.gene057795	PPI
MS.gene057783	MS.gene68162	PPI
MS.gene045365	MS.gene057783	PPI
MS.gene057783	MS.gene045363	PPI
MS.gene057783	MS.gene75675	PPI
MS.gene057783	MS.gene79887	PPI
MS.gene50659	MS.gene057783	PPI
MS.gene75316	MS.gene057783	PPI
MS.gene057783	MS.gene50652	PPI
MS.gene057783	MS.gene68761	PPI
MS.gene057783	MS.gene002380	PPI
MS.gene057790	MS.gene057783	PPI
MS.gene057783	MS.gene68165	PPI
MS.gene003624	MS.gene057783	PPI
MS.gene031201	MS.gene057783	PPI
MS.gene057783	MS.gene50677	PPI
MS.gene057783	MS.gene50684	PPI
MS.gene057783	MS.gene045366	PPI
MS.gene77529	MS.gene057783	PPI
MS.gene014667	MS.gene057783	PPI
MS.gene057783	MS.gene045362	PPI
MS.gene057783	MS.gene23372	PPI
MS.gene057783	MS.gene014668	PPI
MS.gene057783	MS.gene50765	PPI
MS.gene057783	MS.gene003586	PPI
MS.gene057778	MS.gene057783	PPI
MS.gene057783	MS.gene003627	PPI
MS.gene057783	MS.gene79891	PPI
MS.gene057783	MS.gene045367	PPI
MS.gene057783	MS.gene045364	PPI
MS.gene79890	MS.gene057783	PPI
MS.gene003615	MS.gene057783	PPI
MS.gene062973	MS.gene057783	PPI
MS.gene003602	MS.gene057783	PPI
MS.gene057783	MS.gene014669	PPI
MS.gene057783	MS.gene057808	PPI
MS.gene062984	MS.gene057783	PPI
MS.gene40327	MS.gene057783	PPI
MS.gene057783	MS.gene77526	PPI
MS.gene057783	MS.gene68163	PPI
MS.gene057783	MS.gene75315	PPI
MS.gene057783	MS.gene057818	PPI
MS.gene057783	MS.gene68164	PPI
MS.gene77523	MS.gene057783	PPI
MS.gene057783	MS.gene68759	PPI
MS.gene057783	MS.gene79889	PPI
MS.gene003587	MS.gene057783	PPI
MS.gene057783	MS.gene045368	PPI
MS.gene057783	MS.gene031200	PPI
MS.gene057783	MS.gene045360	PPI
MS.gene057783	MS.gene014222	PPI
MS.gene057783	MS.gene79888	PPI
MS.gene003590	MS.gene057783	PPI
MS.gene063112	MS.gene057783	PPI
MS.gene057783	MS.gene75676	PPI
MS.gene057783	MS.gene75678	PPI
MS.gene057783	MS.gene031199	PPI
MS.gene057783	MS.gene50687	PPI
MS.gene057783	MS.gene014223	PPI
MS.gene057783	MS.gene75673	PPI
MS.gene057783	MS.gene79885	PPI
MS.gene047517	MS.gene057783	PPI
MS.gene057783	MS.gene50764	PPI
MS.gene057783	MS.gene062963	PPI
MS.gene75677	MS.gene057783	PPI
MS.gene003625	MS.gene057783	PPI
MS.gene057783	MS.gene003577	PPI
MS.gene057783	MS.gene50675	PPI
MS.gene045361	MS.gene057783	PPI
MS.gene003591	MS.gene057783	PPI
MS.gene057783	MS.gene014670	PPI
MS.gene062979	MS.gene057783	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057783.t1	MTR_4g072820	96.552	87	2	1	1	87	1	86	1.63e-56	169
MS.gene057783.t1	MTR_4g072380	85.542	83	12	0	1	83	1	83	1.63e-47	146
MS.gene057783.t1	MTR_4g072580	82.353	85	14	1	1	85	1	84	3.11e-47	145
MS.gene057783.t1	MTR_4g072740	79.070	86	17	1	1	86	1	85	6.85e-46	142
MS.gene057783.t1	MTR_4g072490	77.907	86	18	1	1	86	1	85	3.92e-45	140
MS.gene057783.t1	MTR_4g072370	77.528	89	16	2	1	86	1	88	1.94e-44	138
MS.gene057783.t1	MTR_4g072870	72.527	91	20	1	1	86	1	91	3.81e-44	137
MS.gene057783.t1	MTR_4g072470	76.744	86	19	1	1	86	1	85	2.80e-43	135
MS.gene057783.t1	MTR_4g072310	72.826	92	18	2	1	86	1	91	5.69e-43	135
MS.gene057783.t1	MTR_4g072260	72.222	90	20	1	1	85	1	90	1.08e-42	134
MS.gene057783.t1	MTR_4g072830	77.273	88	16	2	1	85	1	87	1.20e-42	134
MS.gene057783.t1	MTR_4g072600	77.647	85	18	1	1	85	1	84	1.41e-42	134
MS.gene057783.t1	MTR_3g084150	72.527	91	19	2	1	86	1	90	1.55e-42	134
MS.gene057783.t1	MTR_3g110038	71.111	90	22	1	1	86	1	90	1.79e-42	133
MS.gene057783.t1	MTR_4g072610	78.824	85	16	2	1	85	1	83	1.84e-42	133
MS.gene057783.t1	MTR_4g072880	71.739	92	19	2	1	86	1	91	2.15e-42	133
MS.gene057783.t1	MTR_4g072220	72.222	90	20	1	1	85	1	90	2.20e-42	133
MS.gene057783.t1	MTR_4g072330	70.455	88	24	1	1	86	1	88	2.92e-42	133
MS.gene057783.t1	MTR_3g084170	71.111	90	22	1	1	86	1	90	4.75e-42	132
MS.gene057783.t1	MTR_4g072630	74.419	86	22	0	1	86	1	86	5.84e-42	132
MS.gene057783.t1	MTR_4g072240	70.652	92	20	2	1	86	1	91	9.40e-42	132
MS.gene057783.t1	MTR_3g084180	71.429	91	20	2	1	86	1	90	1.13e-41	131
MS.gene057783.t1	MTR_4g072890	69.231	91	23	1	1	86	1	91	3.14e-41	130
MS.gene057783.t1	MTR_4g072650	77.647	85	17	2	1	85	1	83	3.57e-41	130
MS.gene057783.t1	MTR_3g084240	70.000	90	23	1	1	86	1	90	4.91e-41	130
MS.gene057783.t1	MTR_8g461410	69.231	91	22	2	1	86	1	90	5.19e-41	130
MS.gene057783.t1	MTR_4g050700	69.231	91	23	1	1	86	1	91	1.30e-40	129
MS.gene057783.t1	MTR_2g012410	69.231	91	23	1	1	86	1	91	1.30e-40	129
MS.gene057783.t1	MTR_4g072250	71.429	91	20	2	1	86	1	90	2.00e-40	128
MS.gene057783.t1	MTR_4g072500	72.527	91	19	3	1	86	1	90	2.11e-40	128
MS.gene057783.t1	MTR_4g072465	74.713	87	20	2	1	86	1	86	3.95e-40	127
MS.gene057783.t1	MTR_4g072300	68.478	92	22	2	1	86	1	91	4.72e-40	127
MS.gene057783.t1	MTR_4g072540	75.581	86	21	0	1	86	1	86	1.11e-39	126
MS.gene057783.t1	MTR_3g084220	67.778	90	25	1	1	86	1	90	1.32e-39	126
MS.gene057783.t1	MTR_4g072480	68.132	91	23	2	1	86	1	90	1.47e-39	126
MS.gene057783.t1	MTR_3g084250	71.429	91	20	2	1	86	1	90	2.12e-39	125
MS.gene057783.t1	MTR_4g072230	72.222	90	16	3	1	84	1	87	3.88e-39	125
MS.gene057783.t1	MTR_4g072270	68.539	89	21	2	1	83	1	88	5.18e-39	125
MS.gene057783.t1	MTR_4g072850	73.118	93	17	2	1	86	1	92	5.58e-39	125
MS.gene057783.t1	MTR_4g072530	70.330	91	21	3	1	86	1	90	6.17e-39	124
MS.gene057783.t1	MTR_3g110042	71.111	90	20	2	1	85	1	89	7.15e-39	124
MS.gene057783.t1	MTR_4g072670	74.419	86	22	0	1	86	1	86	7.72e-39	124
MS.gene057783.t1	MTR_4g072570	68.132	91	23	2	1	86	1	90	9.40e-39	124
MS.gene057783.t1	MTR_4g072660	69.880	83	24	1	1	83	1	82	1.57e-38	123
MS.gene057783.t1	MTR_4g072640	69.231	91	22	3	1	86	1	90	1.72e-38	123
MS.gene057783.t1	MTR_3g084260	65.934	91	26	1	1	86	1	91	2.09e-38	123
MS.gene057783.t1	MTR_4g072860	69.565	92	21	2	1	86	1	91	2.57e-38	123
MS.gene057783.t1	MTR_5g008350	77.333	75	16	1	1	75	1	74	3.49e-38	122
MS.gene057783.t1	MTR_3g110032	66.304	92	24	2	1	86	1	91	4.17e-38	122
MS.gene057783.t1	MTR_4g072590	80.233	86	16	1	1	86	1	85	4.34e-38	122
MS.gene057783.t1	MTR_4g072620	64.835	91	26	2	1	86	1	90	4.42e-38	122
MS.gene057783.t1	MTR_4g072680	65.934	91	25	2	1	86	1	90	5.56e-38	122
MS.gene057783.t1	MTR_8g461420	71.111	90	22	1	1	86	1	90	1.07e-37	121
MS.gene057783.t1	MTR_3g117630	71.429	84	17	2	9	86	8	90	1.13e-37	121
MS.gene057783.t1	MTR_4g072730	63.333	90	29	1	1	86	1	90	2.10e-37	120
MS.gene057783.t1	MTR_4g072510	73.256	86	23	0	1	86	1	86	2.76e-37	120
MS.gene057783.t1	MTR_3g084190	68.889	90	24	1	1	86	1	90	4.23e-37	120
MS.gene057783.t1	MTR_3g084230	70.000	90	23	1	1	86	1	90	6.63e-37	119
MS.gene057783.t1	MTR_4g072720	72.093	86	24	0	1	86	1	86	7.32e-37	119
MS.gene057783.t1	MTR_4g072460	63.736	91	27	2	1	86	1	90	1.76e-36	118
MS.gene057783.t1	MTR_3g084210	68.889	90	24	1	1	86	1	90	2.19e-36	118
MS.gene057783.t1	MTR_4g072940	71.429	91	18	2	3	86	33	122	8.14e-36	117
MS.gene057783.t1	MTR_3g110045	70.455	88	22	1	3	86	62	149	1.02e-35	118
MS.gene057783.t1	MTR_3g084160	68.132	91	23	2	1	86	1	90	3.59e-35	115
MS.gene057783.t1	MTR_4g072690	75.581	86	20	1	1	86	1	85	8.68e-35	114
MS.gene057783.t1	MTR_5g008370	66.667	90	26	2	1	86	1	90	1.26e-34	115
MS.gene057783.t1	MTR_4g072770	66.279	86	23	1	1	86	1	80	2.76e-34	112
MS.gene057783.t1	MTR_5g008360	68.354	79	24	1	8	86	4	81	4.95e-34	112
MS.gene057783.t1	MTR_4g072980	57.447	94	33	2	1	87	1	94	2.44e-33	110
MS.gene057783.t1	MTR_4g072450	73.256	86	22	1	1	86	1	85	9.80e-33	108
MS.gene057783.t1	MTR_4g072550	65.000	80	24	1	11	86	22	101	1.02e-31	107
MS.gene057783.t1	MTR_3g084200	71.233	73	15	2	1	68	1	72	2.92e-31	105
MS.gene057783.t1	MTR_4g072560	52.525	99	35	3	1	87	1	99	3.01e-31	105
MS.gene057783.t1	MTR_4g072910	64.103	78	25	1	9	86	26	100	1.31e-30	103
MS.gene057783.t1	MTR_3g117640	52.000	100	34	2	1	86	1	100	9.29e-30	102
MS.gene057783.t1	MTR_4g072840	60.920	87	8	2	1	87	1	61	9.13e-28	95.5
MS.gene057783.t1	MTR_4g072290	54.545	88	27	2	1	86	1	77	2.22e-27	95.1
MS.gene057783.t1	MTR_4g072750	68.000	75	16	2	1	68	1	74	3.08e-27	95.1
MS.gene057783.t1	MTR_3g110035	65.625	64	22	0	23	86	73	136	1.77e-25	92.0
MS.gene057783.t1	MTR_3g113310	59.701	67	26	1	20	86	41	106	1.80e-25	91.3
MS.gene057783.t1	MTR_4g072280	61.333	75	24	2	1	70	1	75	1.17e-24	88.6
MS.gene057783.t1	MTR_4g072930	53.750	80	33	1	11	86	31	110	1.27e-24	89.4
MS.gene057783.t1	MTR_3g117610	62.500	64	23	1	20	83	44	106	1.05e-23	87.0
MS.gene057783.t1	MTR_4g072190	51.765	85	37	2	5	86	17	100	4.15e-23	85.1
MS.gene057783.t1	MTR_6g077850	56.061	66	29	0	20	85	33	98	5.28e-23	84.7
MS.gene057783.t1	MTR_8g076040	55.882	68	29	1	19	86	39	105	2.79e-21	80.5
MS.gene057783.t1	MTR_5g021820	54.412	68	30	1	19	86	38	104	5.78e-21	79.7
MS.gene057783.t1	MTR_8g461400	46.667	90	18	2	1	86	1	64	4.37e-19	73.9
MS.gene057783.t1	MTR_4g124750	50.000	74	36	1	13	86	61	133	1.52e-17	72.4
MS.gene057783.t1	MTR_2g043960	52.941	68	28	2	20	87	75	138	7.94e-17	70.9
MS.gene057783.t1	MTR_1g024085	48.000	75	38	1	4	78	20	93	9.97e-17	70.1
MS.gene057783.t1	MTR_1g094750	51.613	62	29	1	20	81	49	109	1.15e-16	69.3
MS.gene057783.t1	MTR_4g005310	48.529	68	34	1	15	81	56	123	1.66e-16	69.7
MS.gene057783.t1	MTR_1g009150	52.239	67	31	1	12	78	62	127	4.38e-16	69.3
MS.gene057783.t1	MTR_4g025830	47.059	68	32	2	21	85	79	145	1.15e-15	67.8
MS.gene057783.t1	MTR_7g101740	52.308	65	30	1	19	83	38	101	1.37e-15	66.2
MS.gene057783.t1	MTR_8g461210	51.667	60	28	1	19	78	33	91	6.91e-15	64.7
MS.gene057783.t1	MTR_4g005320	52.459	61	28	1	18	78	67	126	8.87e-15	65.5
MS.gene057783.t1	MTR_1g063950	50.000	62	30	1	20	81	47	107	1.09e-14	64.3
MS.gene057783.t1	MTR_7g118010	47.692	65	31	2	21	83	23	86	2.68e-14	62.8
MS.gene057783.t1	MTR_4g072160	50.794	63	28	2	20	81	36	96	7.30e-14	62.4
MS.gene057783.t1	MTR_1g061570	50.000	66	32	1	13	78	11	75	8.87e-14	62.0
MS.gene057783.t1	MTR_3g092220	42.857	77	40	3	4	78	17	91	1.26e-13	61.2
MS.gene057783.t1	MTR_0388s0020	42.647	68	35	2	21	85	75	141	1.63e-13	62.0
MS.gene057783.t1	MTR_7g104950	35.065	77	49	1	11	87	40	115	1.99e-13	61.2
MS.gene057783.t1	MTR_8g026730	48.333	60	28	2	21	78	27	85	2.49e-13	60.5
MS.gene057783.t1	MTR_2g095430	44.118	68	33	2	11	74	35	101	4.72e-13	60.5
MS.gene057783.t1	MTR_1g059770	36.620	71	44	1	17	87	45	114	4.93e-13	60.1
MS.gene057783.t1	MTR_3g109160	51.667	60	28	1	19	78	67	125	6.43e-13	60.8
MS.gene057783.t1	MTR_2g066830	41.667	72	39	2	2	73	41	109	1.03e-12	59.7
MS.gene057783.t1	MTR_3g097120	45.333	75	32	2	20	86	8	81	1.73e-12	58.2
MS.gene057783.t1	MTR_3g105900	72.222	36	10	0	20	55	1171	1206	2.69e-12	60.8
MS.gene057783.t1	MTR_8g096470	43.333	60	33	1	22	81	47	105	6.79e-12	57.0
MS.gene057783.t1	MTR_7g104940	35.000	80	45	2	8	87	43	115	7.11e-12	57.4
MS.gene057783.t1	MTR_4g021810	49.254	67	32	2	13	78	18	83	7.52e-12	56.6
MS.gene057783.t1	MTR_7g405740	39.437	71	42	1	11	81	57	126	1.53e-11	57.0
MS.gene057783.t1	MTR_8g101520	44.615	65	35	1	22	86	51	114	3.38e-11	56.2
MS.gene057783.t1	MTR_8g096440	40.260	77	42	2	7	80	28	103	3.68e-11	55.8
MS.gene057783.t1	MTR_8g096500	45.000	60	32	1	21	80	49	107	6.08e-11	55.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057783.t1	AT4G38840	73.529	68	17	1	20	86	32	99	2.72e-33	110
MS.gene057783.t1	AT5G18050	64.474	76	26	1	12	86	15	90	6.77e-33	109
MS.gene057783.t1	AT5G18020	64.474	76	26	1	12	86	15	90	1.12e-32	108
MS.gene057783.t1	AT5G18080	64.474	76	26	1	12	86	15	90	3.74e-32	107
MS.gene057783.t1	AT5G18060	64.474	76	26	1	12	86	15	90	6.06e-32	107
MS.gene057783.t1	AT5G18030	62.500	80	25	2	8	86	13	88	1.45e-31	106
MS.gene057783.t1	AT4G34800	56.667	90	36	1	1	87	1	90	4.48e-31	105
MS.gene057783.t1	AT4G34810	63.415	82	25	2	10	86	37	118	1.29e-30	104
MS.gene057783.t1	AT4G38850	60.976	82	24	2	8	86	13	89	1.45e-30	103
MS.gene057783.t1	AT2G21200	63.636	77	26	2	10	85	11	86	1.55e-30	103
MS.gene057783.t1	AT5G18010	61.842	76	28	1	12	86	15	90	4.17e-30	102
MS.gene057783.t1	AT2G21210	63.158	76	25	2	12	85	20	94	1.78e-29	101
MS.gene057783.t1	AT4G34770	64.865	74	24	1	14	85	28	101	2.61e-29	100
MS.gene057783.t1	AT4G38825	63.235	68	24	1	20	86	22	89	3.71e-29	100
MS.gene057783.t1	AT2G21210	63.158	76	25	2	12	85	87	161	8.20e-29	101
MS.gene057783.t1	AT3G03840	55.682	88	30	3	1	86	14	94	4.58e-28	97.4
MS.gene057783.t1	AT3G03820	57.143	77	30	1	13	86	19	95	9.48e-28	96.7
MS.gene057783.t1	AT4G13790	50.000	86	40	2	3	86	8	92	6.57e-27	94.4
MS.gene057783.t1	AT3G03850	64.179	67	22	1	22	86	26	92	6.61e-27	94.4
MS.gene057783.t1	AT3G03830	58.333	72	27	1	18	86	21	92	3.21e-26	92.8
MS.gene057783.t1	AT4G34790	63.235	68	23	1	21	86	39	106	1.14e-24	89.4
MS.gene057783.t1	AT4G38860	51.648	91	35	2	5	86	15	105	1.76e-24	88.6
MS.gene057783.t1	AT4G34780	52.500	80	35	1	10	86	17	96	3.60e-24	87.8
MS.gene057783.t1	AT1G75580	62.121	66	24	1	21	86	44	108	6.09e-23	85.1
MS.gene057783.t1	AT2G21220	50.000	90	35	3	5	85	15	103	1.65e-22	83.6
MS.gene057783.t1	AT4G34760	56.716	67	28	1	20	86	42	107	4.57e-22	82.8
MS.gene057783.t1	AT1G19830	61.765	68	24	2	20	86	47	113	4.88e-21	80.5
MS.gene057783.t1	AT4G36110	46.250	80	42	1	4	83	23	101	2.70e-19	75.5
MS.gene057783.t1	AT2G16580	53.731	67	30	1	20	86	43	108	4.06e-19	75.1
MS.gene057783.t1	AT2G18010	56.250	64	27	1	20	83	47	109	2.28e-18	73.6
MS.gene057783.t1	AT3G43120	52.941	68	28	2	20	87	78	141	4.51e-18	73.9
MS.gene057783.t1	AT5G66260	46.988	83	43	1	4	86	17	98	7.68e-18	71.6
MS.gene057783.t1	AT3G51200	45.570	79	37	3	4	81	26	99	3.18e-17	70.5
MS.gene057783.t1	AT3G20220	46.479	71	36	2	12	81	38	107	2.77e-16	68.2
MS.gene057783.t1	AT5G20810	52.941	68	28	2	20	87	78	141	2.82e-16	69.3
MS.gene057783.t1	AT2G37030	53.226	62	28	1	20	81	48	108	3.89e-16	68.2
MS.gene057783.t1	AT5G20810	52.941	68	28	2	20	87	78	141	5.37e-16	69.3
MS.gene057783.t1	AT2G45210	45.205	73	36	2	16	85	74	145	1.04e-15	67.8
MS.gene057783.t1	AT3G60690	46.479	71	34	2	19	86	86	155	1.46e-15	67.8
MS.gene057783.t1	AT1G75590	50.000	68	32	2	12	78	32	98	1.58e-14	64.7
MS.gene057783.t1	AT5G10990	43.529	85	40	3	10	87	29	112	1.60e-14	64.7
MS.gene057783.t1	AT4G34750	46.269	67	35	1	12	78	28	93	1.73e-14	64.3
MS.gene057783.t1	AT4G34750	46.269	67	35	1	12	78	28	93	1.73e-14	64.3
MS.gene057783.t1	AT3G61900	43.662	71	36	2	11	78	20	89	2.63e-14	63.5
MS.gene057783.t1	AT1G19840	50.000	70	33	2	10	78	29	97	7.19e-14	62.8
MS.gene057783.t1	AT3G53250	46.774	62	29	1	20	81	51	108	1.22e-13	61.6
MS.gene057783.t1	AT4G09530	38.095	84	49	2	1	81	8	91	1.29e-13	61.2
MS.gene057783.t1	AT1G56150	44.118	68	37	1	11	78	28	94	2.27e-13	60.5
MS.gene057783.t1	AT2G46690	43.836	73	36	3	9	78	13	83	2.48e-13	60.8
MS.gene057783.t1	AT3G53250	46.774	62	29	1	20	81	82	139	2.53e-13	61.6
MS.gene057783.t1	AT3G12830	43.662	71	37	2	3	73	34	101	4.25e-13	60.5
MS.gene057783.t1	AT2G24400	47.143	70	36	1	9	78	52	120	5.92e-13	61.2
MS.gene057783.t1	AT4G00880	44.872	78	37	3	3	78	13	86	7.38e-13	59.7
MS.gene057783.t1	AT1G16510	40.278	72	42	1	15	86	48	118	1.87e-12	59.3
MS.gene057783.t1	AT4G31320	44.286	70	38	1	9	78	68	136	1.93e-12	60.1
MS.gene057783.t1	AT4G12410	44.118	68	34	2	21	85	76	142	2.27e-12	59.3
MS.gene057783.t1	AT5G03310	47.297	74	29	4	20	87	38	107	6.45e-12	57.0
MS.gene057783.t1	AT3G09870	46.875	64	31	2	12	73	36	98	6.59e-12	57.0
MS.gene057783.t1	AT4G22620	38.554	83	46	3	6	85	65	145	1.18e-11	57.4
MS.gene057783.t1	AT5G53590	42.029	69	34	2	20	83	45	112	2.41e-11	56.2

Find 19 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATCAAGCAGAAGAAGAATT+TGG	0.279041	4.4:+36556001	MS.gene057783:CDS
CTTTCTTACAACTCTCTTCT+CGG	0.306926	4.4:+36556071	MS.gene057783:CDS
GCTTACCTGGTATCATTAGA+AGG	0.376089	4.4:+36555852	MS.gene057783:CDS
TAAACTGCAAGATAGCCTTT+TGG	0.389661	4.4:-36555905	None:intergenic
AGGTTGGTTCAATAATGAAA+TGG	0.405391	4.4:-36555960	None:intergenic
GGTTGGTTCAATAATGAAAT+GGG	0.411568	4.4:-36555959	None:intergenic
GGCTATCTTGCAGTTTATGT+TGG	0.454915	4.4:+36555911	MS.gene057783:CDS
TGTAGTAATTCTTGAAATGA+AGG	0.484527	4.4:-36555980	None:intergenic
TGGGACTTCATCCACAGCCT+TGG	0.495456	4.4:-36555885	None:intergenic
GTAATTCTTGAAATGAAGGT+TGG	0.498263	4.4:-36555976	None:intergenic
GTCATCATCTTCCAAGGCTG+TGG	0.512173	4.4:+36555874	MS.gene057783:CDS
TTTGGATATGATCATCCAAT+GGG	0.541874	4.4:+36556019	MS.gene057783:CDS
ATTTGGATATGATCATCCAA+TGG	0.556875	4.4:+36556018	MS.gene057783:CDS
AAGAAAGCATCTTCACTGCA+TGG	0.581745	4.4:-36556055	None:intergenic
TAGAAGGTCATCATCTTCCA+AGG	0.592917	4.4:+36555868	MS.gene057783:CDS
GCTGTGGATGAAGTCCCAAA+AGG	0.598635	4.4:+36555890	MS.gene057783:CDS
GGAATTGTGAGACCACCCAT+TGG	0.604006	4.4:-36556034	None:intergenic
GATGACCTTCTAATGATACC+AGG	0.644342	4.4:-36555857	None:intergenic
GGATATGATCATCCAATGGG+TGG	0.655640	4.4:+36556022	MS.gene057783:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGTAGTAATTCTTGAAATGA+AGG	-	chr4.4:36555983-36556002	None:intergenic	25.0%
	ATTTGGATATGATCATCCAA+TGG	+	chr4.4:36556018-36556037	MS.gene057783:CDS	30.0%
	GTAATTCTTGAAATGAAGGT+TGG	-	chr4.4:36555979-36555998	None:intergenic	30.0%
	TTTGGATATGATCATCCAAT+GGG	+	chr4.4:36556019-36556038	MS.gene057783:CDS	30.0%
!	AGGTTGGTTCAATAATGAAA+TGG	-	chr4.4:36555963-36555982	None:intergenic	30.0%
!	GGTTGGTTCAATAATGAAAT+GGG	-	chr4.4:36555962-36555981	None:intergenic	30.0%
	CATCAAGCAGAAGAAGAATT+TGG	+	chr4.4:36556001-36556020	MS.gene057783:CDS	35.0%
	CTTTCTTACAACTCTCTTCT+CGG	+	chr4.4:36556071-36556090	MS.gene057783:CDS	35.0%
!	AAACTGCAAGATAGCCTTTT+GGG	-	chr4.4:36555907-36555926	None:intergenic	35.0%
!	TAAACTGCAAGATAGCCTTT+TGG	-	chr4.4:36555908-36555927	None:intergenic	35.0%
	GATGACCTTCTAATGATACC+AGG	-	chr4.4:36555860-36555879	None:intergenic	40.0%
	GCTTACCTGGTATCATTAGA+AGG	+	chr4.4:36555852-36555871	MS.gene057783:CDS	40.0%
	GGCTATCTTGCAGTTTATGT+TGG	+	chr4.4:36555911-36555930	MS.gene057783:CDS	40.0%
	TAGAAGGTCATCATCTTCCA+AGG	+	chr4.4:36555868-36555887	MS.gene057783:CDS	40.0%
!!	AAGAAAGCATCTTCACTGCA+TGG	-	chr4.4:36556058-36556077	None:intergenic	40.0%
	GGATATGATCATCCAATGGG+TGG	+	chr4.4:36556022-36556041	MS.gene057783:CDS	45.0%
	GCTGTGGATGAAGTCCCAAA+AGG	+	chr4.4:36555890-36555909	MS.gene057783:CDS	50.0%
	GGAATTGTGAGACCACCCAT+TGG	-	chr4.4:36556037-36556056	None:intergenic	50.0%
	GTCATCATCTTCCAAGGCTG+TGG	+	chr4.4:36555874-36555893	MS.gene057783:CDS	50.0%
	TGGGACTTCATCCACAGCCT+TGG	-	chr4.4:36555888-36555907	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	36555842	36556105	36555842	ID=MS.gene057783
chr4.4	mRNA	36555842	36556105	36555842	ID=MS.gene057783.t1;Parent=MS.gene057783
chr4.4	exon	36555842	36556105	36555842	ID=MS.gene057783.t1.exon1;Parent=MS.gene057783.t1
chr4.4	CDS	36555842	36556105	36555842	ID=cds.MS.gene057783.t1;Parent=MS.gene057783.t1

Gene Sequence	>MS.gene057783 ATGGGTTTTCGCTTACCTGGTATCATTAGAAGGTCATCATCTTCCAAGGCTGTGGATGAAGTCCCAAAAGGCTATCTTGCAGTTTATGTTGGAGAAAAAATGAAGCGATTTGTGATTCCCATTTCATTATTGAACCAACCTTCATTTCAAGAATTACTACATCAAGCAGAAGAAGAATTTGGATATGATCATCCAATGGGTGGTCTCACAATTCCATGCAGTGAAGATGCTTTCTTACAACTCTCTTCTCGGCTTCAATGTTAA
Protein sequence	>MS.gene057783.t1 MGFRLPGIIRRSSSSKAVDEVPKGYLAVYVGEKMKRFVIPISLLNQPSFQELLHQAEEEFGYDHPMGGLTIPCSEDAFLQLSSRLQC