AlfalfaGEDB | MS.gene057784

M. sativa cultivar XinJiangDaYe / MS.gene057784

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057784.t1	AES89311.1	85.6	90	13	0	1	90	1	90	3.40E-36	160.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057784.t1	P33080	70.0	90	25	1	1	88	1	90	1.1e-30	133.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057784.t1	G7JL36	85.6	90	13	0	1	90	1	90	2.4e-36	160.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057784	MS.gene062983	0.821096	4.84E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene057784	MS.gene79887	PPI
MS.gene045360	MS.gene057784	PPI
MS.gene057784	MS.gene75679	PPI
MS.gene057784	MS.gene68761	PPI
MS.gene057784	MS.gene40325	PPI
MS.gene75677	MS.gene057784	PPI
MS.gene003625	MS.gene057784	PPI
MS.gene057784	MS.gene50684	PPI
MS.gene057784	MS.gene057797	PPI
MS.gene057784	MS.gene003614	PPI
MS.gene045361	MS.gene057784	PPI
MS.gene057784	MS.gene014668	PPI
MS.gene057784	MS.gene79886	PPI
MS.gene057784	MS.gene50765	PPI
MS.gene50675	MS.gene057784	PPI
MS.gene057784	MS.gene003596	PPI
MS.gene003591	MS.gene057784	PPI
MS.gene057784	MS.gene23371	PPI
MS.gene062979	MS.gene057784	PPI
MS.gene01358	MS.gene057784	PPI
MS.gene057784	MS.gene003583	PPI
MS.gene057784	MS.gene062951	PPI
MS.gene057784	MS.gene77527	PPI
MS.gene057784	MS.gene79891	PPI
MS.gene057784	MS.gene057795	PPI
MS.gene057784	MS.gene045367	PPI
MS.gene057784	MS.gene045364	PPI
MS.gene40326	MS.gene057784	PPI
MS.gene057784	MS.gene045363	PPI
MS.gene062952	MS.gene057784	PPI
MS.gene50676	MS.gene057784	PPI
MS.gene057784	MS.gene50652	PPI
MS.gene057784	MS.gene014669	PPI
MS.gene057784	MS.gene057808	PPI
MS.gene057784	MS.gene002380	PPI
MS.gene23370	MS.gene057784	PPI
MS.gene50766	MS.gene057784	PPI
MS.gene77529	MS.gene057784	PPI
MS.gene014667	MS.gene057784	PPI
MS.gene003594	MS.gene057784	PPI
MS.gene057784	MS.gene68165	PPI
MS.gene50665	MS.gene057784	PPI
MS.gene50677	MS.gene057784	PPI
MS.gene003586	MS.gene057784	PPI
MS.gene062988	MS.gene057784	PPI
MS.gene75673	MS.gene057784	PPI
MS.gene057784	MS.gene68163	PPI
MS.gene057784	MS.gene75315	PPI
MS.gene057784	MS.gene057818	PPI
MS.gene057784	MS.gene68164	PPI
MS.gene50763	MS.gene057784	PPI
MS.gene057784	MS.gene68759	PPI
MS.gene045365	MS.gene057784	PPI
MS.gene057784	MS.gene031200	PPI
MS.gene057784	MS.gene79888	PPI
MS.gene50659	MS.gene057784	PPI
MS.gene057784	MS.gene75676	PPI
MS.gene75316	MS.gene057784	PPI
MS.gene057784	MS.gene031199	PPI
MS.gene057790	MS.gene057784	PPI
MS.gene057784	MS.gene50687	PPI
MS.gene057784	MS.gene014223	PPI
MS.gene003624	MS.gene057784	PPI
MS.gene057784	MS.gene79885	PPI
MS.gene031201	MS.gene057784	PPI
MS.gene77523	MS.gene057784	PPI
MS.gene057778	MS.gene057784	PPI
MS.gene045366	MS.gene057784	PPI
MS.gene062963	MS.gene057784	PPI
MS.gene057784	MS.gene79889	PPI
MS.gene057784	MS.gene045368	PPI
MS.gene75674	MS.gene057784	PPI
MS.gene79890	MS.gene057784	PPI
MS.gene003615	MS.gene057784	PPI
MS.gene057784	MS.gene014222	PPI
MS.gene062973	MS.gene057784	PPI
MS.gene003602	MS.gene057784	PPI
MS.gene23372	MS.gene057784	PPI
MS.gene047517	MS.gene057784	PPI
MS.gene057784	MS.gene75678	PPI
MS.gene057784	MS.gene75314	PPI
MS.gene057784	MS.gene01764	PPI
MS.gene003627	MS.gene057784	PPI
MS.gene062984	MS.gene057784	PPI
MS.gene40327	MS.gene057784	PPI
MS.gene057784	MS.gene68760	PPI
MS.gene057784	MS.gene031198	PPI
MS.gene057784	MS.gene50764	PPI
MS.gene057784	MS.gene77528	PPI
MS.gene045362	MS.gene057784	PPI
MS.gene057784	MS.gene014671	PPI
MS.gene003587	MS.gene057784	PPI
MS.gene057784	MS.gene003577	PPI
MS.gene057784	MS.gene75672	PPI
MS.gene003590	MS.gene057784	PPI
MS.gene062943	MS.gene057784	PPI
MS.gene063112	MS.gene057784	PPI
MS.gene057784	MS.gene50654	PPI
MS.gene057784	MS.gene062974	PPI
MS.gene003608	MS.gene057784	PPI
MS.gene057784	MS.gene014670	PPI
MS.gene057784	MS.gene057801	PPI
MS.gene057784	MS.gene77524	PPI
MS.gene057784	MS.gene68162	PPI
MS.gene057784	MS.gene75675	PPI
MS.gene77526	MS.gene057784	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057784.t1	MTR_4g072370	85.556	90	13	0	1	90	1	90	1.02e-53	162
MS.gene057784.t1	MTR_4g072330	82.222	90	16	0	1	90	1	90	2.77e-52	158
MS.gene057784.t1	MTR_4g072830	85.057	87	13	0	1	87	1	87	4.09e-50	153
MS.gene057784.t1	MTR_4g072580	80.000	90	15	1	1	90	1	87	1.31e-47	146
MS.gene057784.t1	MTR_4g072740	80.682	88	14	1	1	88	1	85	1.90e-47	146
MS.gene057784.t1	MTR_4g072600	80.000	90	15	1	1	90	1	87	2.17e-47	146
MS.gene057784.t1	MTR_4g072630	76.136	88	19	1	1	88	1	86	6.93e-45	139
MS.gene057784.t1	MTR_4g072490	75.000	88	19	1	1	88	1	85	3.61e-44	137
MS.gene057784.t1	MTR_4g072870	71.429	91	23	1	1	88	1	91	2.44e-43	135
MS.gene057784.t1	MTR_4g072470	77.273	88	17	1	1	88	1	85	3.35e-43	135
MS.gene057784.t1	MTR_4g072880	68.132	91	26	1	1	88	1	91	1.58e-42	134
MS.gene057784.t1	MTR_3g084150	70.000	90	25	1	1	88	1	90	1.87e-42	134
MS.gene057784.t1	MTR_3g084250	72.222	90	23	1	1	88	1	90	1.96e-42	134
MS.gene057784.t1	MTR_4g072240	69.231	91	25	1	1	88	1	91	2.29e-42	133
MS.gene057784.t1	MTR_3g110032	70.330	91	24	1	1	88	1	91	2.86e-42	133
MS.gene057784.t1	MTR_4g072310	70.330	91	24	1	1	88	1	91	9.89e-42	132
MS.gene057784.t1	MTR_3g084180	68.889	90	26	1	1	88	1	90	1.04e-41	132
MS.gene057784.t1	MTR_4g072300	68.132	91	26	1	1	88	1	91	2.22e-41	131
MS.gene057784.t1	MTR_3g084170	71.111	90	24	1	1	88	1	90	2.36e-41	130
MS.gene057784.t1	MTR_3g110038	71.111	90	24	1	1	88	1	90	2.44e-41	130
MS.gene057784.t1	MTR_4g072610	70.000	90	23	1	1	90	1	86	3.00e-41	130
MS.gene057784.t1	MTR_4g072230	72.527	91	20	2	1	88	1	89	4.38e-41	130
MS.gene057784.t1	MTR_4g072570	68.889	90	26	1	1	88	1	90	5.67e-41	130
MS.gene057784.t1	MTR_4g072465	70.455	88	24	1	1	88	1	86	6.12e-41	129
MS.gene057784.t1	MTR_8g461410	67.778	90	27	1	1	88	1	90	6.47e-41	130
MS.gene057784.t1	MTR_4g072650	72.222	90	21	2	1	90	1	86	1.07e-40	129
MS.gene057784.t1	MTR_4g072540	73.864	88	21	1	1	88	1	86	1.66e-40	128
MS.gene057784.t1	MTR_3g084240	70.000	90	25	1	1	88	1	90	2.75e-40	128
MS.gene057784.t1	MTR_4g072220	68.132	91	26	1	1	88	1	91	3.03e-40	128
MS.gene057784.t1	MTR_4g072860	71.429	91	23	1	1	88	1	91	4.60e-40	127
MS.gene057784.t1	MTR_4g072670	75.000	88	20	1	1	88	1	86	5.03e-40	127
MS.gene057784.t1	MTR_4g072890	67.033	91	27	1	1	88	1	91	5.76e-40	127
MS.gene057784.t1	MTR_4g050700	70.330	91	24	1	1	88	1	91	6.05e-40	127
MS.gene057784.t1	MTR_2g012410	70.330	91	24	1	1	88	1	91	6.05e-40	127
MS.gene057784.t1	MTR_4g072500	70.330	91	23	2	1	88	1	90	6.13e-40	127
MS.gene057784.t1	MTR_4g072250	71.429	91	22	2	1	88	1	90	7.00e-40	127
MS.gene057784.t1	MTR_4g072820	74.157	89	18	3	1	88	1	85	2.24e-39	125
MS.gene057784.t1	MTR_4g072510	72.727	88	22	1	1	88	1	86	2.50e-39	125
MS.gene057784.t1	MTR_4g072640	70.330	91	23	2	1	88	1	90	2.70e-39	125
MS.gene057784.t1	MTR_4g072260	65.934	91	28	1	1	88	1	91	2.97e-39	125
MS.gene057784.t1	MTR_3g084220	67.778	90	27	1	1	88	1	90	3.39e-39	125
MS.gene057784.t1	MTR_4g072530	70.330	91	23	2	1	88	1	90	4.03e-39	125
MS.gene057784.t1	MTR_4g072270	64.835	91	29	1	1	88	1	91	1.75e-38	124
MS.gene057784.t1	MTR_4g072380	70.000	90	23	2	1	89	1	87	8.41e-38	122
MS.gene057784.t1	MTR_3g084260	68.132	91	26	1	1	88	1	91	1.05e-37	122
MS.gene057784.t1	MTR_3g117630	67.045	88	26	1	4	88	3	90	1.74e-37	121
MS.gene057784.t1	MTR_3g084190	65.556	90	29	1	1	88	1	90	3.88e-37	120
MS.gene057784.t1	MTR_8g461420	66.667	90	28	1	1	88	1	90	3.96e-37	120
MS.gene057784.t1	MTR_4g072850	68.478	92	25	1	1	88	1	92	4.64e-37	120
MS.gene057784.t1	MTR_4g072720	69.318	88	25	1	1	88	1	86	4.67e-37	120
MS.gene057784.t1	MTR_4g072730	63.333	90	31	1	1	88	1	90	7.24e-37	119
MS.gene057784.t1	MTR_4g072480	62.222	90	32	1	1	88	1	90	8.92e-37	119
MS.gene057784.t1	MTR_3g084210	64.444	90	30	1	1	88	1	90	1.74e-36	118
MS.gene057784.t1	MTR_3g084230	65.556	90	29	1	1	88	1	90	1.99e-36	118
MS.gene057784.t1	MTR_4g072590	70.455	88	23	1	1	88	1	85	3.57e-36	117
MS.gene057784.t1	MTR_4g072680	61.111	90	33	1	1	88	1	90	5.15e-36	117
MS.gene057784.t1	MTR_3g110042	67.778	90	25	2	1	87	1	89	5.50e-36	117
MS.gene057784.t1	MTR_5g008350	74.026	77	17	1	1	77	1	74	6.19e-36	117
MS.gene057784.t1	MTR_4g072450	70.455	88	23	1	1	88	1	85	6.52e-36	117
MS.gene057784.t1	MTR_4g072660	63.636	88	29	1	1	88	1	85	7.80e-36	117
MS.gene057784.t1	MTR_4g072620	60.000	90	34	1	1	88	1	90	9.42e-36	117
MS.gene057784.t1	MTR_3g110045	67.045	88	27	1	3	88	62	149	1.31e-35	118
MS.gene057784.t1	MTR_4g072460	60.000	90	34	1	1	88	1	90	2.88e-35	115
MS.gene057784.t1	MTR_3g084160	65.934	91	27	2	1	88	1	90	3.25e-35	115
MS.gene057784.t1	MTR_4g072690	69.318	88	24	1	1	88	1	85	3.46e-35	115
MS.gene057784.t1	MTR_4g072940	67.778	90	25	1	3	88	33	122	2.73e-34	114
MS.gene057784.t1	MTR_5g008370	63.333	90	31	1	1	88	1	90	1.74e-33	112
MS.gene057784.t1	MTR_5g008360	62.963	81	30	0	8	88	1	81	1.87e-33	110
MS.gene057784.t1	MTR_4g072910	65.333	75	25	1	14	88	27	100	6.49e-31	105
MS.gene057784.t1	MTR_4g072560	52.000	100	36	3	1	89	1	99	1.64e-30	103
MS.gene057784.t1	MTR_3g084200	68.056	72	21	1	1	70	1	72	1.33e-29	101
MS.gene057784.t1	MTR_3g117640	49.020	102	40	2	1	90	1	102	4.24e-29	100
MS.gene057784.t1	MTR_4g072550	63.380	71	26	0	18	88	31	101	5.61e-29	100
MS.gene057784.t1	MTR_4g072770	68.182	66	21	0	23	88	15	80	5.88e-29	99.4
MS.gene057784.t1	MTR_4g072980	53.409	88	37	2	1	87	8	92	9.51e-29	99.4
MS.gene057784.t1	MTR_4g072280	64.000	75	24	1	1	72	1	75	2.11e-28	97.8
MS.gene057784.t1	MTR_4g072290	55.682	88	28	2	1	88	1	77	5.54e-26	91.7
MS.gene057784.t1	MTR_4g072750	61.429	70	27	0	1	70	5	74	1.36e-25	90.9
MS.gene057784.t1	MTR_3g110035	56.790	81	29	1	8	88	62	136	3.95e-25	91.3
MS.gene057784.t1	MTR_6g077850	56.716	67	29	0	22	88	33	99	2.09e-24	88.6
MS.gene057784.t1	MTR_4g072190	50.000	86	37	2	7	87	15	99	5.61e-23	84.7
MS.gene057784.t1	MTR_4g072930	51.220	82	35	2	13	89	30	111	7.11e-23	85.1
MS.gene057784.t1	MTR_3g113310	56.716	67	28	1	21	87	40	105	1.14e-22	84.3
MS.gene057784.t1	MTR_3g117610	60.000	65	25	1	21	85	43	106	1.54e-22	84.0
MS.gene057784.t1	MTR_8g461400	48.889	90	18	2	1	88	1	64	2.81e-20	77.0
MS.gene057784.t1	MTR_1g094750	54.839	62	27	1	22	83	49	109	6.91e-19	75.1
MS.gene057784.t1	MTR_2g043960	45.556	90	37	2	2	80	44	132	1.54e-18	75.1
MS.gene057784.t1	MTR_5g021820	47.436	78	40	1	10	87	27	103	2.01e-18	73.6
MS.gene057784.t1	MTR_4g072840	46.591	88	19	2	1	88	1	60	2.23e-18	72.0
MS.gene057784.t1	MTR_1g063950	53.226	62	28	1	22	83	47	107	2.31e-18	73.9
MS.gene057784.t1	MTR_8g076040	46.067	89	36	2	8	85	15	102	4.56e-18	72.4
MS.gene057784.t1	MTR_7g101740	56.250	64	27	1	22	85	39	101	4.88e-18	72.8
MS.gene057784.t1	MTR_8g461210	57.627	59	24	1	22	80	34	91	5.66e-18	72.8
MS.gene057784.t1	MTR_4g124750	55.932	59	25	1	22	80	68	125	4.82e-17	71.2
MS.gene057784.t1	MTR_1g061570	53.226	62	28	1	22	83	18	78	1.90e-16	68.9
MS.gene057784.t1	MTR_4g025830	47.887	71	33	2	20	87	76	145	2.34e-16	69.7
MS.gene057784.t1	MTR_3g092220	42.308	78	42	2	14	89	24	100	3.56e-16	67.8
MS.gene057784.t1	MTR_4g072160	47.297	74	36	2	11	83	25	96	4.86e-16	68.2
MS.gene057784.t1	MTR_4g005310	53.226	62	28	1	23	83	62	123	8.09e-16	68.2
MS.gene057784.t1	MTR_1g024085	44.872	78	38	2	3	80	21	93	1.04e-15	67.8
MS.gene057784.t1	MTR_7g118010	45.588	68	34	2	23	88	23	89	2.02e-15	65.9
MS.gene057784.t1	MTR_0388s0020	41.026	78	42	2	13	87	65	141	5.43e-15	65.9
MS.gene057784.t1	MTR_2g066830	41.558	77	44	1	13	89	48	123	1.65e-14	64.3
MS.gene057784.t1	MTR_1g009150	45.946	74	39	1	10	83	58	130	1.95e-14	65.1
MS.gene057784.t1	MTR_8g026730	44.118	68	35	2	23	88	27	93	4.49e-14	62.4
MS.gene057784.t1	MTR_8g096470	45.000	60	32	1	24	83	47	105	1.15e-13	61.6
MS.gene057784.t1	MTR_7g405740	47.541	61	31	1	23	83	67	126	3.00e-13	61.6
MS.gene057784.t1	MTR_8g096440	39.189	74	44	1	10	83	32	104	4.66e-13	60.8
MS.gene057784.t1	MTR_4g005320	50.000	58	28	1	23	80	70	126	1.09e-12	60.1
MS.gene057784.t1	MTR_8g096500	45.902	61	32	1	23	83	49	108	1.73e-12	59.7
MS.gene057784.t1	MTR_3g109160	50.820	61	29	1	23	83	69	128	1.82e-12	59.7
MS.gene057784.t1	MTR_3g098970	34.211	76	49	1	13	88	43	117	8.62e-12	57.4
MS.gene057784.t1	MTR_8g101520	43.077	65	36	1	24	88	51	114	9.34e-12	57.8
MS.gene057784.t1	MTR_5g008380	78.125	32	7	0	57	88	246	277	1.75e-11	58.5
MS.gene057784.t1	MTR_1g059770	38.462	65	39	1	23	87	49	112	2.42e-11	55.8
MS.gene057784.t1	MTR_2g095430	41.096	73	38	2	8	76	30	101	2.86e-11	55.8
MS.gene057784.t1	MTR_7g101890	38.571	70	40	2	9	76	38	106	5.46e-11	54.7
MS.gene057784.t1	MTR_3g105900	63.415	41	15	0	22	62	1171	1211	6.64e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057784.t1	AT4G38840	69.118	68	20	1	22	88	32	99	1.14e-31	107
MS.gene057784.t1	AT5G18080	58.667	75	30	1	15	88	16	90	4.84e-29	100
MS.gene057784.t1	AT5G18020	57.317	82	30	2	8	88	13	90	5.09e-29	100
MS.gene057784.t1	AT2G21210	58.108	74	29	1	17	88	22	95	1.11e-28	99.4
MS.gene057784.t1	AT4G34770	62.963	81	28	1	10	88	22	102	1.11e-28	99.4
MS.gene057784.t1	AT2G21200	57.500	80	33	1	9	87	7	86	1.49e-28	98.6
MS.gene057784.t1	AT5G18060	56.098	82	31	2	8	88	13	90	1.67e-28	98.6
MS.gene057784.t1	AT5G18050	54.878	82	32	2	8	88	13	90	2.32e-28	98.2
MS.gene057784.t1	AT3G03840	61.111	72	26	1	19	88	23	94	3.18e-28	98.2
MS.gene057784.t1	AT4G38850	54.762	84	28	2	8	88	13	89	4.54e-28	97.4
MS.gene057784.t1	AT2G21210	58.108	74	29	1	17	88	89	162	5.09e-28	99.8
MS.gene057784.t1	AT4G38825	57.333	75	31	1	15	88	15	89	5.60e-28	97.4
MS.gene057784.t1	AT4G34810	65.714	70	19	2	23	87	48	117	1.14e-27	97.4
MS.gene057784.t1	AT4G34800	60.811	74	26	1	13	83	11	84	1.40e-27	96.3
MS.gene057784.t1	AT5G18030	59.722	72	28	1	18	88	17	88	1.41e-27	96.3
MS.gene057784.t1	AT5G18010	56.000	75	32	1	15	88	16	90	1.92e-27	95.9
MS.gene057784.t1	AT3G03820	52.222	90	40	1	2	88	6	95	4.10e-27	95.1
MS.gene057784.t1	AT3G03850	61.194	67	24	1	24	88	26	92	1.46e-25	91.3
MS.gene057784.t1	AT4G34780	62.857	70	23	1	21	87	26	95	2.07e-25	91.3
MS.gene057784.t1	AT4G13790	47.126	87	41	2	3	87	8	91	2.13e-25	90.9
MS.gene057784.t1	AT4G34790	60.870	69	25	1	23	89	39	107	2.44e-24	88.6
MS.gene057784.t1	AT4G38860	55.882	68	30	0	21	88	38	105	1.53e-22	84.0
MS.gene057784.t1	AT3G03830	50.000	74	34	1	18	88	19	92	4.35e-22	82.4
MS.gene057784.t1	AT2G37030	56.338	71	30	1	14	84	40	109	2.69e-21	81.3
MS.gene057784.t1	AT1G75580	58.209	67	27	1	21	87	42	107	8.88e-21	79.7
MS.gene057784.t1	AT2G21220	45.977	87	39	2	8	87	18	103	1.79e-20	78.6
MS.gene057784.t1	AT4G34760	46.067	89	38	2	8	87	19	106	2.09e-20	78.6
MS.gene057784.t1	AT3G43120	53.030	66	30	1	15	80	71	135	2.57e-19	77.4
MS.gene057784.t1	AT3G12830	44.318	88	44	2	2	89	33	115	4.09e-18	73.6
MS.gene057784.t1	AT1G19830	58.333	60	24	1	21	80	46	104	7.77e-18	72.4
MS.gene057784.t1	AT5G20810	53.030	66	30	1	15	80	71	135	1.19e-17	73.2
MS.gene057784.t1	AT2G16580	44.318	88	38	2	8	85	19	105	1.22e-17	71.6
MS.gene057784.t1	AT5G20810	53.030	66	30	1	15	80	71	135	1.52e-17	73.6
MS.gene057784.t1	AT3G53250	50.794	63	27	1	22	84	51	109	2.56e-17	71.2
MS.gene057784.t1	AT3G53250	50.794	63	27	1	22	84	82	140	4.16e-17	71.6
MS.gene057784.t1	AT4G36110	53.125	64	29	1	22	85	39	101	4.63e-17	70.1
MS.gene057784.t1	AT5G66260	43.023	86	43	2	8	88	14	98	7.35e-17	69.3
MS.gene057784.t1	AT3G20220	49.275	69	33	2	23	90	47	114	1.00e-16	69.7
MS.gene057784.t1	AT2G18010	51.562	64	30	1	22	85	47	109	6.24e-16	67.4
MS.gene057784.t1	AT4G34750	43.478	69	38	1	22	90	36	103	6.84e-16	68.2
MS.gene057784.t1	AT4G34750	43.478	69	38	1	22	90	36	103	6.84e-16	68.2
MS.gene057784.t1	AT1G75590	52.542	59	27	1	22	80	41	98	1.83e-15	67.0
MS.gene057784.t1	AT1G16510	49.254	67	33	1	23	89	54	119	2.01e-15	67.0
MS.gene057784.t1	AT5G10990	47.143	70	36	1	11	80	29	97	4.59e-15	65.9
MS.gene057784.t1	AT3G61900	45.570	79	38	3	14	88	20	97	5.12e-15	65.5
MS.gene057784.t1	AT1G56150	46.269	67	35	1	23	89	38	103	7.15e-15	64.7
MS.gene057784.t1	AT3G51200	50.794	63	29	2	22	83	38	99	7.32e-15	64.3
MS.gene057784.t1	AT1G19840	52.542	59	27	1	22	80	40	97	1.61e-14	64.7
MS.gene057784.t1	AT5G03310	51.471	68	26	3	22	83	38	104	1.85e-14	63.5
MS.gene057784.t1	AT2G46690	40.506	79	43	2	13	88	14	91	4.16e-14	62.8
MS.gene057784.t1	AT4G12410	42.500	80	42	2	11	87	64	142	1.17e-13	62.8
MS.gene057784.t1	AT2G45210	42.466	73	38	2	18	87	74	145	2.19e-13	62.0
MS.gene057784.t1	AT3G60690	44.928	69	34	2	23	88	88	155	2.22e-13	62.0
MS.gene057784.t1	AT1G79130	41.250	80	41	2	8	87	38	111	4.06e-13	60.8
MS.gene057784.t1	AT4G00880	53.333	60	25	2	23	80	28	86	1.36e-12	58.9
MS.gene057784.t1	AT4G22620	44.118	68	34	2	23	87	79	145	2.15e-12	59.3
MS.gene057784.t1	AT5G53590	41.667	72	36	2	22	88	45	115	4.00e-12	58.2
MS.gene057784.t1	AT3G09870	49.206	63	29	2	16	76	38	99	1.46e-11	56.2
MS.gene057784.t1	AT4G31320	47.541	61	31	1	23	83	80	139	1.81e-11	57.4
MS.gene057784.t1	AT2G24400	49.180	61	30	1	23	83	64	123	2.47e-11	57.0
MS.gene057784.t1	AT2G36210	33.735	83	55	0	8	90	39	121	6.09e-11	55.1

Find 22 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTCACCTGGTATCATTAGA+AGG	0.300177	4.4:-36568209	MS.gene057784:CDS
AATCAAGCTGAGGAACAATT+TGG	0.330842	4.4:-36568054	MS.gene057784:CDS
GCAATTCTTGAAACAATGGT+TGG	0.491830	4.4:+36568079	None:intergenic
TCAAAACAAGCATCTTCAAA+AGG	0.508704	4.4:-36568183	MS.gene057784:CDS
TTCAGCAATTCTTGAAACAA+TGG	0.512470	4.4:+36568075	None:intergenic
TTTGGATATGATCATCCAAC+GGG	0.521338	4.4:-36568036	MS.gene057784:CDS
AAGAAAACGTCTTCTCTACA+AGG	0.526143	4.4:+36568000	None:intergenic
GGATACCTTGCAGTATATGT+TGG	0.528219	4.4:-36568144	MS.gene057784:CDS
AAAAGGCATGGATGTGCCAA+AGG	0.533320	4.4:-36568166	MS.gene057784:CDS
ACAAGCATCTTCAAAAGGCA+TGG	0.535432	4.4:-36568178	MS.gene057784:CDS
TTCACCTGGTATCATTAGAA+GGG	0.554199	4.4:-36568208	MS.gene057784:CDS
TATACTGCAAGGTATCCCTT+TGG	0.555072	4.4:+36568150	None:intergenic
GAGGCCCTTCTAATGATACC+AGG	0.577589	4.4:+36568204	None:intergenic
AAAGGCATGGATGTGCCAAA+GGG	0.593194	4.4:-36568165	MS.gene057784:CDS
CCTTGCAGTATATGTTGGAG+AGG	0.605007	4.4:-36568139	MS.gene057784:CDS
CCTCTCCAACATATACTGCA+AGG	0.627279	4.4:+36568139	None:intergenic
ATTTGGATATGATCATCCAA+CGG	0.628419	4.4:-36568037	MS.gene057784:CDS
AGAATTGCTGAATCAAGCTG+AGG	0.644376	4.4:-36568064	MS.gene057784:CDS
AGAAAACGTCTTCTCTACAA+GGG	0.654570	4.4:+36568001	None:intergenic
GGGATTGTGAGACCACCCGT+TGG	0.658136	4.4:+36568021	None:intergenic
ATGTTGGAGAGGAAATGAAG+CGG	0.662900	4.4:-36568128	MS.gene057784:CDS
GGATATGATCATCCAACGGG+TGG	0.666271	4.4:-36568033	MS.gene057784:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATTTGGATATGATCATCCAA+CGG	-	chr4.4:36568151-36568170	MS.gene057784:CDS	30.0%
	TCAAAACAAGCATCTTCAAA+AGG	-	chr4.4:36568005-36568024	MS.gene057784:CDS	30.0%
	TTCAGCAATTCTTGAAACAA+TGG	+	chr4.4:36568116-36568135	None:intergenic	30.0%
!!!	TTTTGAAGATGCTTGTTTTG+AGG	+	chr4.4:36568006-36568025	None:intergenic	30.0%
	AAGAAAACGTCTTCTCTACA+AGG	+	chr4.4:36568191-36568210	None:intergenic	35.0%
	AATCAAGCTGAGGAACAATT+TGG	-	chr4.4:36568134-36568153	MS.gene057784:CDS	35.0%
	AGAAAACGTCTTCTCTACAA+GGG	+	chr4.4:36568190-36568209	None:intergenic	35.0%
	GCAATTCTTGAAACAATGGT+TGG	+	chr4.4:36568112-36568131	None:intergenic	35.0%
	TTCACCTGGTATCATTAGAA+GGG	-	chr4.4:36567980-36567999	MS.gene057784:CDS	35.0%
	TTTGGATATGATCATCCAAC+GGG	-	chr4.4:36568152-36568171	MS.gene057784:CDS	35.0%
	ACAAGCATCTTCAAAAGGCA+TGG	-	chr4.4:36568010-36568029	MS.gene057784:CDS	40.0%
	AGAATTGCTGAATCAAGCTG+AGG	-	chr4.4:36568124-36568143	MS.gene057784:CDS	40.0%
	ATGTTGGAGAGGAAATGAAG+CGG	-	chr4.4:36568060-36568079	MS.gene057784:CDS	40.0%
	GGATACCTTGCAGTATATGT+TGG	-	chr4.4:36568044-36568063	MS.gene057784:CDS	40.0%
	GTTCACCTGGTATCATTAGA+AGG	-	chr4.4:36567979-36567998	MS.gene057784:CDS	40.0%
!	TATACTGCAAGGTATCCCTT+TGG	+	chr4.4:36568041-36568060	None:intergenic	40.0%
	CCTCTCCAACATATACTGCA+AGG	+	chr4.4:36568052-36568071	None:intergenic	45.0%
	CCTTGCAGTATATGTTGGAG+AGG	-	chr4.4:36568049-36568068	MS.gene057784:CDS	45.0%
!	AAAAGGCATGGATGTGCCAA+AGG	-	chr4.4:36568022-36568041	MS.gene057784:CDS	45.0%
!	AAAGGCATGGATGTGCCAAA+GGG	-	chr4.4:36568023-36568042	MS.gene057784:CDS	45.0%
	GAGGCCCTTCTAATGATACC+AGG	+	chr4.4:36567987-36568006	None:intergenic	50.0%
	GGATATGATCATCCAACGGG+TGG	-	chr4.4:36568155-36568174	MS.gene057784:CDS	50.0%
	GGGATTGTGAGACCACCCGT+TGG	+	chr4.4:36568170-36568189	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	36567969	36568241	36567969	ID=MS.gene057784
chr4.4	mRNA	36567969	36568241	36567969	ID=MS.gene057784.t1;Parent=MS.gene057784
chr4.4	exon	36567969	36568241	36567969	ID=MS.gene057784.t1.exon1;Parent=MS.gene057784.t1
chr4.4	CDS	36567969	36568241	36567969	ID=cds.MS.gene057784.t1;Parent=MS.gene057784.t1

Gene Sequence	>MS.gene057784 ATGGGTTTTCGTTCACCTGGTATCATTAGAAGGGCCTCAAAACAAGCATCTTCAAAAGGCATGGATGTGCCAAAGGGATACCTTGCAGTATATGTTGGAGAGGAAATGAAGCGGTTTGTGATATCAATTTCTTTGTTGAACCAACCATTGTTTCAAGAATTGCTGAATCAAGCTGAGGAACAATTTGGATATGATCATCCAACGGGTGGTCTCACAATCCCTTGTAGAGAAGACGTTTTCTTAGATGTTACTTCTCGTTTAAATCTGTGCTAA
Protein sequence	>MS.gene057784.t1 MGFRSPGIIRRASKQASSKGMDVPKGYLAVYVGEEMKRFVISISLLNQPLFQELLNQAEEQFGYDHPTGGLTIPCREDVFLDVTSRLNLC