Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064937.t1 | XP_024630994.1 | 96.7 | 61 | 2 | 0 | 1 | 61 | 20 | 80 | 1.10E-22 | 115.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064937.t1 | Q9T0H2 | 59.7 | 62 | 24 | 1 | 1 | 61 | 1 | 62 | 1.8e-10 | 65.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064937.t1 | A0A1S2XYN4 | 73.8 | 61 | 15 | 1 | 1 | 61 | 1 | 60 | 7.9e-15 | 88.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene064937 | 0.832816 | 7.62E-56 | -1.69E-46 |
| MS.gene049255 | MS.gene064937 | 0.855054 | 8.16E-62 | -1.69E-46 |
| MS.gene049341 | MS.gene064937 | 0.819884 | 9.19E-53 | -1.69E-46 |
| MS.gene049653 | MS.gene064937 | 0.813742 | 2.19E-51 | -1.69E-46 |
| MS.gene049756 | MS.gene064937 | 0.822841 | 1.91E-53 | -1.69E-46 |
| MS.gene049821 | MS.gene064937 | 0.804073 | 2.57E-49 | -1.69E-46 |
| MS.gene050228 | MS.gene064937 | 0.80367 | 3.12E-49 | -1.69E-46 |
| MS.gene050277 | MS.gene064937 | 0.827743 | 1.32E-54 | -1.69E-46 |
| MS.gene050303 | MS.gene064937 | 0.834188 | 3.46E-56 | -1.69E-46 |
| MS.gene050442 | MS.gene064937 | 0.800683 | 1.28E-48 | -1.69E-46 |
| MS.gene050623 | MS.gene064937 | -0.81458 | 1.43E-51 | -1.69E-46 |
| MS.gene050689 | MS.gene064937 | 0.80328 | 3.76E-49 | -1.69E-46 |
| MS.gene050780 | MS.gene064937 | 0.801481 | 8.82E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene064937 | 0.827224 | 1.76E-54 | -1.69E-46 |
| MS.gene050815 | MS.gene064937 | 0.855462 | 6.21E-62 | -1.69E-46 |
| MS.gene051241 | MS.gene064937 | 0.802309 | 5.96E-49 | -1.69E-46 |
| MS.gene051243 | MS.gene064937 | 0.854156 | 1.48E-61 | -1.69E-46 |
| MS.gene051245 | MS.gene064937 | 0.822946 | 1.80E-53 | -1.69E-46 |
| MS.gene051328 | MS.gene064937 | 0.849337 | 3.45E-60 | -1.69E-46 |
| MS.gene051349 | MS.gene064937 | 0.815252 | 1.02E-51 | -1.69E-46 |
| MS.gene05134 | MS.gene064937 | 0.84627 | 2.41E-59 | -1.69E-46 |
| MS.gene051451 | MS.gene064937 | 0.819504 | 1.12E-52 | -1.69E-46 |
| MS.gene051452 | MS.gene064937 | 0.832926 | 7.15E-56 | -1.69E-46 |
| MS.gene051535 | MS.gene064937 | 0.813121 | 3.00E-51 | -1.69E-46 |
| MS.gene051789 | MS.gene064937 | 0.80885 | 2.53E-50 | -1.69E-46 |
| MS.gene051975 | MS.gene064937 | 0.812031 | 5.20E-51 | -1.69E-46 |
| MS.gene052012 | MS.gene064937 | 0.841732 | 3.98E-58 | -1.69E-46 |
| MS.gene052286 | MS.gene064937 | 0.801469 | 8.87E-49 | -1.69E-46 |
| MS.gene052376 | MS.gene064937 | 0.8045 | 2.10E-49 | -1.69E-46 |
| MS.gene052426 | MS.gene064937 | 0.819017 | 1.45E-52 | -1.69E-46 |
| MS.gene053006 | MS.gene064937 | 0.807817 | 4.20E-50 | -1.69E-46 |
| MS.gene053008 | MS.gene064937 | 0.849265 | 3.61E-60 | -1.69E-46 |
| MS.gene053058 | MS.gene064937 | 0.812435 | 4.25E-51 | -1.69E-46 |
| MS.gene053074 | MS.gene064937 | 0.826851 | 2.16E-54 | -1.69E-46 |
| MS.gene053080 | MS.gene064937 | 0.80002 | 1.75E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene064937 | 0.801736 | 7.82E-49 | -1.69E-46 |
| MS.gene053931 | MS.gene064937 | 0.801413 | 9.11E-49 | -1.69E-46 |
| MS.gene054472 | MS.gene064937 | 0.81791 | 2.58E-52 | -1.69E-46 |
| MS.gene054657 | MS.gene064937 | 0.852322 | 4.98E-61 | -1.69E-46 |
| MS.gene054658 | MS.gene064937 | 0.839743 | 1.32E-57 | -1.69E-46 |
| MS.gene05510 | MS.gene064937 | 0.829641 | 4.59E-55 | -1.69E-46 |
| MS.gene055162 | MS.gene064937 | 0.807826 | 4.18E-50 | -1.69E-46 |
| MS.gene05517 | MS.gene064937 | 0.852585 | 4.19E-61 | -1.69E-46 |
| MS.gene055512 | MS.gene064937 | 0.800643 | 1.31E-48 | -1.69E-46 |
| MS.gene055623 | MS.gene064937 | 0.861213 | 1.20E-63 | -1.69E-46 |
| MS.gene055701 | MS.gene064937 | 0.802293 | 6.01E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene064937 | 0.813475 | 2.51E-51 | -1.69E-46 |
| MS.gene055841 | MS.gene064937 | 0.81002 | 1.42E-50 | -1.69E-46 |
| MS.gene056506 | MS.gene064937 | 0.810029 | 1.41E-50 | -1.69E-46 |
| MS.gene056631 | MS.gene064937 | 0.83022 | 3.32E-55 | -1.69E-46 |
| MS.gene056760 | MS.gene064937 | 0.841428 | 4.78E-58 | -1.69E-46 |
| MS.gene057060 | MS.gene064937 | 0.826723 | 2.32E-54 | -1.69E-46 |
| MS.gene05712 | MS.gene064937 | 0.804701 | 1.90E-49 | -1.69E-46 |
| MS.gene057140 | MS.gene064937 | 0.842507 | 2.48E-58 | -1.69E-46 |
| MS.gene057208 | MS.gene064937 | 0.808404 | 3.15E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene064937 | 0.860467 | 2.02E-63 | -1.69E-46 |
| MS.gene058084 | MS.gene064937 | 0.820223 | 7.68E-53 | -1.69E-46 |
| MS.gene058126 | MS.gene064937 | 0.801297 | 9.62E-49 | -1.69E-46 |
| MS.gene058146 | MS.gene064937 | 0.813757 | 2.18E-51 | -1.69E-46 |
| MS.gene058238 | MS.gene064937 | 0.811011 | 8.66E-51 | -1.69E-46 |
| MS.gene058548 | MS.gene064937 | 0.808273 | 3.36E-50 | -1.69E-46 |
| MS.gene058595 | MS.gene064937 | 0.815702 | 8.07E-52 | -1.69E-46 |
| MS.gene058906 | MS.gene064937 | 0.832524 | 9.00E-56 | -1.69E-46 |
| MS.gene059006 | MS.gene064937 | 0.816606 | 5.07E-52 | -1.69E-46 |
| MS.gene059317 | MS.gene064937 | 0.802112 | 6.55E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene064937 | 0.800201 | 1.61E-48 | -1.69E-46 |
| MS.gene059569 | MS.gene064937 | 0.834173 | 3.49E-56 | -1.69E-46 |
| MS.gene05978 | MS.gene064937 | 0.811483 | 6.84E-51 | -1.69E-46 |
| MS.gene05979 | MS.gene064937 | 0.820938 | 5.26E-53 | -1.69E-46 |
| MS.gene059830 | MS.gene064937 | 0.853894 | 1.77E-61 | -1.69E-46 |
| MS.gene059842 | MS.gene064937 | 0.830559 | 2.74E-55 | -1.69E-46 |
| MS.gene060310 | MS.gene064937 | 0.816924 | 4.30E-52 | -1.69E-46 |
| MS.gene060322 | MS.gene064937 | 0.814949 | 1.19E-51 | -1.69E-46 |
| MS.gene060429 | MS.gene064937 | 0.830193 | 3.37E-55 | -1.69E-46 |
| MS.gene060572 | MS.gene064937 | 0.802044 | 6.76E-49 | -1.69E-46 |
| MS.gene060683 | MS.gene064937 | 0.822308 | 2.54E-53 | -1.69E-46 |
| MS.gene060685 | MS.gene064937 | 0.821176 | 4.64E-53 | -1.69E-46 |
| MS.gene060802 | MS.gene064937 | 0.817793 | 2.74E-52 | -1.69E-46 |
| MS.gene060803 | MS.gene064937 | 0.858339 | 8.82E-63 | -1.69E-46 |
| MS.gene060998 | MS.gene064937 | 0.822716 | 2.04E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064937.t1 | MTR_1g045890 | 94.286 | 35 | 2 | 0 | 1 | 35 | 20 | 54 | 6.88e-17 | 67.4 |
| MS.gene064937.t1 | MTR_1g045890 | 75.000 | 48 | 11 | 1 | 1 | 47 | 20 | 67 | 1.01e-16 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCGACTTTGCTATCATTGA+CGG | 0.418660 | 1.4:-30454733 | None:intergenic |
| ATGAGAACAATGGATTTGTT+CGG | 0.449405 | 1.4:+30454627 | MS.gene064937:CDS |
| ATCATTGACGGCAATACCTT+CGG | 0.457365 | 1.4:-30454721 | None:intergenic |
| GGATTTGTTCGGAGAGGAGA+TGG | 0.472087 | 1.4:+30454638 | MS.gene064937:CDS |
| TCAGATAACAACCATGGCTA+TGG | 0.492608 | 1.4:+30454665 | MS.gene064937:CDS |
| CAGCATCGTCGAAATCGTCT+TGG | 0.535487 | 1.4:-30454779 | None:intergenic |
| AACAATGGATTTGTTCGGAG+AGG | 0.601463 | 1.4:+30454632 | MS.gene064937:CDS |
| AATTTCGATATCTTATCCGA+AGG | 0.608255 | 1.4:+30454705 | MS.gene064937:CDS |
| ATCGTCGAAATCGTCTTGGA+AGG | 0.611833 | 1.4:-30454775 | None:intergenic |
| TTTGTTCGGAGAGGAGATGG+AGG | 0.616272 | 1.4:+30454641 | MS.gene064937:CDS |
| GTCATCAACATCCATAGCCA+TGG | 0.620045 | 1.4:-30454676 | None:intergenic |
| TAGTCGAAGAATGAGAACAA+TGG | 0.638863 | 1.4:+30454617 | None:intergenic |
| GGAGGATCAGATAACAACCA+TGG | 0.690661 | 1.4:+30454659 | MS.gene064937:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATTTCGATATCTTATCCGA+AGG | + | chr1.4:30454705-30454724 | MS.gene064937:CDS | 30.0% | |
| ! | ATGAGAACAATGGATTTGTT+CGG | + | chr1.4:30454627-30454646 | MS.gene064937:CDS | 30.0% |
| ATCATTGACGGCAATACCTT+CGG | - | chr1.4:30454724-30454743 | None:intergenic | 40.0% | |
| TCAGATAACAACCATGGCTA+TGG | + | chr1.4:30454665-30454684 | MS.gene064937:CDS | 40.0% | |
| ! | AACAATGGATTTGTTCGGAG+AGG | + | chr1.4:30454632-30454651 | MS.gene064937:CDS | 40.0% |
| ! | AGCGACTTTGCTATCATTGA+CGG | - | chr1.4:30454736-30454755 | None:intergenic | 40.0% |
| ATCGTCGAAATCGTCTTGGA+AGG | - | chr1.4:30454778-30454797 | None:intergenic | 45.0% | |
| GGAGGATCAGATAACAACCA+TGG | + | chr1.4:30454659-30454678 | MS.gene064937:CDS | 45.0% | |
| GTCATCAACATCCATAGCCA+TGG | - | chr1.4:30454679-30454698 | None:intergenic | 45.0% | |
| CAGCATCGTCGAAATCGTCT+TGG | - | chr1.4:30454782-30454801 | None:intergenic | 50.0% | |
| TTTGTTCGGAGAGGAGATGG+AGG | + | chr1.4:30454641-30454660 | MS.gene064937:CDS | 50.0% | |
| ! | GGATTTGTTCGGAGAGGAGA+TGG | + | chr1.4:30454638-30454657 | MS.gene064937:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 30454627 | 30454812 | 30454627 | ID=MS.gene064937 |
| chr1.4 | mRNA | 30454627 | 30454812 | 30454627 | ID=MS.gene064937.t1;Parent=MS.gene064937 |
| chr1.4 | exon | 30454627 | 30454812 | 30454627 | ID=MS.gene064937.t1.exon1;Parent=MS.gene064937.t1 |
| chr1.4 | CDS | 30454627 | 30454812 | 30454627 | ID=cds.MS.gene064937.t1;Parent=MS.gene064937.t1 |
| Gene Sequence |
| Protein sequence |
