AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071408


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071408.t1 MTR_3g088160 98.450 129 2 0 1 129 308 436 3.08e-86 258
MS.gene071408.t1 MTR_3g088160 96.250 80 3 0 1 80 308 387 1.30e-48 160
MS.gene071408.t1 MTR_5g022510 61.538 52 18 1 3 54 173 222 4.27e-14 67.0
MS.gene071408.t1 MTR_5g022510 61.538 52 18 1 3 54 198 247 6.44e-14 66.6
MS.gene071408.t1 MTR_4g073410 62.745 51 17 1 4 54 200 248 1.03e-13 66.2
MS.gene071408.t1 MTR_4g061140 63.830 47 17 0 2 48 166 212 7.20e-13 63.2
MS.gene071408.t1 MTR_4g061140 63.830 47 17 0 2 48 200 246 8.93e-13 63.2
MS.gene071408.t1 MTR_4g061140 63.830 47 17 0 2 48 200 246 9.19e-13 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071408.t1 AT1G77490 77.344 128 25 1 1 128 293 416 2.07e-62 196
MS.gene071408.t1 AT1G77490 77.344 128 25 1 1 128 293 416 2.16e-62 196
MS.gene071408.t1 AT4G08390 84.746 59 9 0 1 59 314 372 3.02e-27 103
MS.gene071408.t1 AT4G08390 84.746 59 9 0 1 59 314 372 3.02e-27 103
MS.gene071408.t1 AT4G08390 84.746 59 9 0 1 59 314 372 3.02e-27 103
MS.gene071408.t1 AT4G08390 84.746 59 9 0 1 59 313 371 3.59e-27 103
MS.gene071408.t1 AT4G08390 80.435 46 9 0 14 59 301 346 1.34e-17 77.4
MS.gene071408.t1 AT3G09640 65.957 47 16 0 2 48 200 246 2.06e-13 65.1
MS.gene071408.t1 AT3G09640 65.957 47 16 0 2 48 200 246 2.06e-13 65.1
MS.gene071408.t1 AT4G35000 57.692 52 20 1 2 53 197 246 3.68e-12 62.0
MS.gene071408.t1 AT1G07890 63.830 47 17 0 2 48 200 246 5.01e-12 61.2
MS.gene071408.t1 AT1G07890 63.830 47 17 0 2 48 200 246 5.21e-12 61.2
MS.gene071408.t1 AT1G07890 63.830 47 17 0 2 48 200 246 5.21e-12 61.2
MS.gene071408.t1 AT1G07890 63.830 47 17 0 2 48 200 246 5.21e-12 61.2
MS.gene071408.t1 AT1G07890 63.830 47 17 0 2 48 200 246 5.21e-12 61.2
MS.gene071408.t1 AT1G07890 63.830 47 17 0 2 48 200 246 5.21e-12 61.2
MS.gene071408.t1 AT1G07890 63.830 47 17 0 2 48 200 246 5.21e-12 61.2
MS.gene071408.t1 AT1G07890 63.830 47 17 0 2 48 200 246 5.21e-12 61.2

Find 26 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTCCAAGGTTGCTGAGTT+TGG 0.256056 3.3:-73453379 None:intergenic
CATAATTGTTATTGCTGCTT+TGG 0.283340 3.3:+73454104 MS.gene071408:CDS
TTTGACTTTAGAGCCTTATC+TGG 0.287367 3.3:-73454066 None:intergenic
CAATAACAATTATGATGTTT+AGG 0.296597 3.3:-73454095 None:intergenic
AACTTCTCTCCCACTACATT+TGG 0.336185 3.3:-73453648 None:intergenic
TTTCAGGGCATTGTAATAGA+TGG 0.363698 3.3:+73453621 MS.gene071408:intron
AACCTTTCCAGAGGAGTACT+TGG 0.382167 3.3:-73453679 None:intergenic
CAGGTTCAAACTTTGCTCCA+AGG 0.468266 3.3:-73453392 None:intergenic
CATGCCAAACTCAGCAACCT+TGG 0.474570 3.3:+73453375 MS.gene071408:CDS
ATGCTATGAGGAAGAAGATA+CGG 0.479276 3.3:+73454016 MS.gene071408:CDS
CAACGTGAACAGACCTCTGC+AGG 0.481098 3.3:-73453411 None:intergenic
TTGACTTTAGAGCCTTATCT+GGG 0.486207 3.3:-73454065 None:intergenic
TGAGAAATATGCTGTAGATC+AGG 0.501966 3.3:+73453323 MS.gene071408:CDS
GAAGATACGGGCTGAATATG+AGG 0.502305 3.3:+73454029 MS.gene071408:CDS
GCTGAATATGAGGCGGTTGG+CGG 0.508251 3.3:+73454039 MS.gene071408:CDS
GTAGCAGCCAAGTACTCCTC+TGG 0.510718 3.3:+73453672 MS.gene071408:CDS
GGTTGGCGGAAGCCCAGATA+AGG 0.547496 3.3:+73454053 MS.gene071408:CDS
TGCTATGAGGAAGAAGATAC+GGG 0.568469 3.3:+73454017 MS.gene071408:CDS
AAGAGCTGTCTGATGCTATG+AGG 0.570930 3.3:+73454004 MS.gene071408:CDS
AGATGGTTCTCCAAATGTAG+TGG 0.571290 3.3:+73453638 MS.gene071408:CDS
CGGGCTGAATATGAGGCGGT+TGG 0.594896 3.3:+73454036 MS.gene071408:CDS
AGCAAAGTTTGAACCTGCAG+AGG 0.602470 3.3:+73453398 MS.gene071408:CDS
GATGGTTCTCCAAATGTAGT+GGG 0.610180 3.3:+73453639 MS.gene071408:CDS
AGCCAAGTACTCCTCTGGAA+AGG 0.627771 3.3:+73453677 MS.gene071408:CDS
CAAGCTTAAAACCTTTCCAG+AGG 0.659132 3.3:-73453688 None:intergenic
GATACGGGCTGAATATGAGG+CGG 0.710767 3.3:+73454032 MS.gene071408:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAAGAAAACTTTAAT+AGG - chr3.3:73453596-73453615 None:intergenic 10.0%
!!! AATTTTATGTTGAATAAATA+TGG - chr3.3:73453254-73453273 None:intergenic 10.0%
!!! ATATTTCACAAATAATTTTA+TGG - chr3.3:73453291-73453310 None:intergenic 10.0%
!!! TATTTCACAAATAATTTTAT+GGG - chr3.3:73453290-73453309 None:intergenic 10.0%
!!! ATTATTATCACATGTAATTT+TGG + chr3.3:73453922-73453941 MS.gene071408:intron 15.0%
!! AAATCTTAAATGAAGAAGAA+AGG + chr3.3:73453945-73453964 MS.gene071408:intron 20.0%
!! AAATTATTTGTGAAATATGC+AGG + chr3.3:73453293-73453312 MS.gene071408:intron 20.0%
!! CAATAACAATTATGATGTTT+AGG - chr3.3:73454098-73454117 None:intergenic 20.0%
!!! TCTTTTTTATCTGTTTTTCA+GGG + chr3.3:73453606-73453625 MS.gene071408:intron 20.0%
!!! TTCTTTTTTATCTGTTTTTC+AGG + chr3.3:73453605-73453624 MS.gene071408:intron 20.0%
! ATGTTGAATAAATATGGACA+TGG - chr3.3:73453248-73453267 None:intergenic 25.0%
! GAAAACTTTAATAGGTGATT+TGG - chr3.3:73453588-73453607 None:intergenic 25.0%
! TGTTGAATAAATATGGACAT+GGG - chr3.3:73453247-73453266 None:intergenic 25.0%
!!! TGTTTTGTGCATTTTTAGAT+GGG + chr3.3:73453440-73453459 MS.gene071408:intron 25.0%
ATGCAGACATGAAAAAGAAA+AGG - chr3.3:73453524-73453543 None:intergenic 30.0%
! ATTCAGCAATTTTACCTTGA+AGG - chr3.3:73453219-73453238 None:intergenic 30.0%
! CATAATTGTTATTGCTGCTT+TGG + chr3.3:73454104-73454123 MS.gene071408:CDS 30.0%
! TTCATTTTCTTGATGCTTGT+TGG + chr3.3:73453733-73453752 MS.gene071408:intron 30.0%
!! AAGAAGCTATTTTCAAATCG+TGG + chr3.3:73453898-73453917 MS.gene071408:intron 30.0%
!! AGAAGCTATTTTCAAATCGT+GGG + chr3.3:73453899-73453918 MS.gene071408:intron 30.0%
!! CTGTTTTGTGCATTTTTAGA+TGG + chr3.3:73453439-73453458 MS.gene071408:intron 30.0%
!!! GCTATTTTGACATATCTGTT+TGG + chr3.3:73454126-73454145 MS.gene071408:CDS 30.0%
ATGCTATGAGGAAGAAGATA+CGG + chr3.3:73454016-73454035 MS.gene071408:CDS 35.0%
TGAGAAATATGCTGTAGATC+AGG + chr3.3:73453323-73453342 MS.gene071408:CDS 35.0%
! AGCAATTTTACCTTGAAGGA+AGG - chr3.3:73453215-73453234 None:intergenic 35.0%
! GTTCATTGTTTCTCTATTCC+TGG + chr3.3:73453813-73453832 MS.gene071408:intron 35.0%
!! TTGACTTTAGAGCCTTATCT+GGG - chr3.3:73454068-73454087 None:intergenic 35.0%
!! TTTCAGGGCATTGTAATAGA+TGG + chr3.3:73453621-73453640 MS.gene071408:intron 35.0%
!! TTTGACTTTAGAGCCTTATC+TGG - chr3.3:73454069-73454088 None:intergenic 35.0%
AACTTCTCTCCCACTACATT+TGG - chr3.3:73453651-73453670 None:intergenic 40.0%
AGATGGTTCTCCAAATGTAG+TGG + chr3.3:73453638-73453657 MS.gene071408:CDS 40.0%
CAAGCTTAAAACCTTTCCAG+AGG - chr3.3:73453691-73453710 None:intergenic 40.0%
GATGGTTCTCCAAATGTAGT+GGG + chr3.3:73453639-73453658 MS.gene071408:CDS 40.0%
TCAAAAAGAGCAGCATCAGT+TGG - chr3.3:73453188-73453207 None:intergenic 40.0%
TGCTATGAGGAAGAAGATAC+GGG + chr3.3:73454017-73454036 MS.gene071408:CDS 40.0%
! TTTTACTCCTGCATCAAACC+AGG - chr3.3:73453834-73453853 None:intergenic 40.0%
! TTTTGACGATCCTTCCTTCA+AGG + chr3.3:73453202-73453221 MS.gene071408:CDS 40.0%
AAGAGCTGTCTGATGCTATG+AGG + chr3.3:73454004-73454023 MS.gene071408:CDS 45.0%
AGCAAAGTTTGAACCTGCAG+AGG + chr3.3:73453398-73453417 MS.gene071408:CDS 45.0%
CAGGTTCAAACTTTGCTCCA+AGG - chr3.3:73453395-73453414 None:intergenic 45.0%
! CTCTATTCCTGGTTTGATGC+AGG + chr3.3:73453824-73453843 MS.gene071408:intron 45.0%
! GAAGATACGGGCTGAATATG+AGG + chr3.3:73454029-73454048 MS.gene071408:CDS 45.0%
!! AACCTTTCCAGAGGAGTACT+TGG - chr3.3:73453682-73453701 None:intergenic 45.0%
!! ATTATATATAATTTAATGAA+AGG + chr3.3:73453762-73453781 MS.gene071408:intron 5.0%
CATGCCAAACTCAGCAACCT+TGG + chr3.3:73453375-73453394 MS.gene071408:CDS 50.0%
TGCTCCAAGGTTGCTGAGTT+TGG - chr3.3:73453382-73453401 None:intergenic 50.0%
! AGCCAAGTACTCCTCTGGAA+AGG + chr3.3:73453677-73453696 MS.gene071408:CDS 50.0%
! GATACGGGCTGAATATGAGG+CGG + chr3.3:73454032-73454051 MS.gene071408:CDS 50.0%
CAACGTGAACAGACCTCTGC+AGG - chr3.3:73453414-73453433 None:intergenic 55.0%
GCTGAATATGAGGCGGTTGG+CGG + chr3.3:73454039-73454058 MS.gene071408:CDS 55.0%
GTAGCAGCCAAGTACTCCTC+TGG + chr3.3:73453672-73453691 MS.gene071408:CDS 55.0%
CGGGCTGAATATGAGGCGGT+TGG + chr3.3:73454036-73454055 MS.gene071408:CDS 60.0%
GGTTGGCGGAAGCCCAGATA+AGG + chr3.3:73454053-73454072 MS.gene071408:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 73453164 73454155 73453164 ID=MS.gene071408
chr3.3 mRNA 73453164 73454155 73453164 ID=MS.gene071408.t1;Parent=MS.gene071408
chr3.3 exon 73453164 73453223 73453164 ID=MS.gene071408.t1.exon1;Parent=MS.gene071408.t1
chr3.3 CDS 73453164 73453223 73453164 ID=cds.MS.gene071408.t1;Parent=MS.gene071408.t1
chr3.3 exon 73453315 73453419 73453315 ID=MS.gene071408.t1.exon2;Parent=MS.gene071408.t1
chr3.3 CDS 73453315 73453419 73453315 ID=cds.MS.gene071408.t1;Parent=MS.gene071408.t1
chr3.3 exon 73453627 73453698 73453627 ID=MS.gene071408.t1.exon3;Parent=MS.gene071408.t1
chr3.3 CDS 73453627 73453698 73453627 ID=cds.MS.gene071408.t1;Parent=MS.gene071408.t1
chr3.3 exon 73454003 73454155 73454003 ID=MS.gene071408.t1.exon4;Parent=MS.gene071408.t1
chr3.3 CDS 73454003 73454155 73454003 ID=cds.MS.gene071408.t1;Parent=MS.gene071408.t1
Gene Sequence

>MS.gene071408

GATGAAGATCTATTGGTATTGCCAACTGATGCTGCTCTTTTTGACGATCCTTCCTTCAAGGTAAAATTGCTGAATTTTTTCCCATGTCCATATTTATTCAACATAAAATTAAACTGTGATATACCCATAAAATTATTTGTGAAATATGCAGGTATATGCTGAGAAATATGCTGTAGATCAGGAAGCATTCTTTAAAGATTATGCTGAAGCACATGCCAAACTCAGCAACCTTGGAGCAAAGTTTGAACCTGCAGAGGTCTGTTCACGTTGAGTTACTGTTTTGTGCATTTTTAGATGGGTGTGTTTATTATGTTACTACTGACTTAGATATTATGTTCTTTCATACAAAATTTGTTACCTTTTCTTTTTCATGTCTGCATACGTACGTATTTGATTTGAGTTCAAATAACAAGACAGTTGACCAAATCACCTATTAAAGTTTTCTTTTTTATCTGTTTTTCAGGGCATTGTAATAGATGGTTCTCCAAATGTAGTGGGAGAGAAGTTTGTAGCAGCCAAGTACTCCTCTGGAAAGGTTTTAAGCTTGCACAATTTATTAAATTTTACTATTCATTTTCTTGATGCTTGTTGGAAATTTATTATATATAATTTAATGAAAGGTTTGCATAAAATATATATTTTGCATATTGTTCATTGTTTCTCTATTCCTGGTTTGATGCAGGAGTAAAAACAAGTGTGTTGTTTTCTTGTGCAAGTATCTTTAATAATACAAGAAGAAGCTATTTTCAAATCGTGGGATTATTATCACATGTAATTTTGGAAATCTTAAATGAAGAAGAAAGGAATAAAATTCAATAGTGTGTCTATTTGAATTGCAGAAAGAGCTGTCTGATGCTATGAGGAAGAAGATACGGGCTGAATATGAGGCGGTTGGCGGAAGCCCAGATAAGGCTCTAAAGTCAAACTATTTCCTAAACATCATAATTGTTATTGCTGCTTTGGCTATTTTGACATATCTGTTTGGAAACTAA

Protein sequence

>MS.gene071408.t1

DEDLLVLPTDAALFDDPSFKVYAEKYAVDQEAFFKDYAEAHAKLSNLGAKFEPAEGIVIDGSPNVVGEKFVAAKYSSGKKELSDAMRKKIRAEYEAVGGSPDKALKSNYFLNIIIVIAALAILTYLFGN