AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49665


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGGAGAGCTGGAAATTC+TGG 0.279686 5.1:+27825920 MS.gene49665:intron
TAAACAAACCTGTTGTGAAT+AGG 0.400300 5.1:-27824263 None:intergenic
TTAGTTAGTGGTGGAGGAAC+AGG 0.433994 5.1:+27824092 MS.gene49665:CDS
TTTAGTAGCTTAGTTAGTGG+TGG 0.443725 5.1:+27824083 None:intergenic
TGTCAACCATGATGCACAAT+TGG 0.454537 5.1:+27824196 MS.gene49665:CDS
GACCAGATCCTATTCACAAC+AGG 0.455835 5.1:+27824255 MS.gene49665:CDS
CATGATGAACAAGTTGTTGT+TGG 0.456308 5.1:+27824155 MS.gene49665:CDS
TGAATAGGATCTGGTCCATT+GGG 0.478116 5.1:-27824248 None:intergenic
GGAAATTCTGGTAGACCTCC+TGG 0.500969 5.1:+27825932 MS.gene49665:CDS
AGTAGCTTAGTTAGTGGTGG+AGG 0.503163 5.1:+27824086 None:intergenic
AACCTGTTGTGAATAGGATC+TGG 0.513984 5.1:-27824257 None:intergenic
GTGAATAGGATCTGGTCCAT+TGG 0.561168 5.1:-27824249 None:intergenic
TGAAGTCCAATTGTGCATCA+TGG 0.582511 5.1:-27824202 None:intergenic
TCAGTTGACTAAGTTTGACC+AGG 0.583639 5.1:-27825950 None:intergenic
AGTTGTTGTTGGTAAAGACA+AGG 0.593670 5.1:+27824166 MS.gene49665:CDS
GACAAACAACTACAAGACAT+TGG 0.623172 5.1:+27824114 MS.gene49665:CDS
AACTACAAGACATTGGTCAG+AGG 0.649811 5.1:+27824121 MS.gene49665:CDS
GTTGTTGTTGGTAAAGACAA+GGG 0.652393 5.1:+27824167 MS.gene49665:CDS
AGTAAAAGAAGAGTTCCCAA+TGG 0.653384 5.1:+27824233 MS.gene49665:CDS
GTTGACTAAGTTTGACCAGG+AGG 0.677258 5.1:-27825947 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATACAATATTTAGAAAAT+AGG + chr5.1:27824501-27824520 MS.gene49665:intron 10.0%
!! TTTGAAGATATTTAATATAT+AGG - chr5.1:27825631-27825650 None:intergenic 10.0%
!!! TTTGGATTTTTTTTTTTTTT+TGG + chr5.1:27825008-27825027 MS.gene49665:intron 10.0%
!! AAAAAAAGAGATAAAAACTA+AGG + chr5.1:27824879-27824898 MS.gene49665:intron 15.0%
!! AAAAAAGAGATAAAAACTAA+GGG + chr5.1:27824880-27824899 MS.gene49665:intron 15.0%
!! ATTATAGATGAATAATATGT+TGG - chr5.1:27825840-27825859 None:intergenic 15.0%
!! TATCTGTTAATCTATTATAA+AGG - chr5.1:27825265-27825284 None:intergenic 15.0%
!! TGACAAATTTATTTAATGTA+AGG - chr5.1:27825537-27825556 None:intergenic 15.0%
!!! GAATTTTGAAATAGATATTT+AGG - chr5.1:27825505-27825524 None:intergenic 15.0%
!!! TCTTTTTTTTTTTTTTTGAG+TGG - chr5.1:27824868-27824887 None:intergenic 15.0%
!! AAGAAAGACAAAAAAAAAGA+AGG - chr5.1:27825406-27825425 None:intergenic 20.0%
!! AATCTAATTCGATTAGATTA+GGG + chr5.1:27824727-27824746 MS.gene49665:intron 20.0%
!! ATTAAATATCTTCAAATGCT+TGG + chr5.1:27825635-27825654 MS.gene49665:intron 20.0%
!! CACAAATTATTAACAAATTC+AGG + chr5.1:27825575-27825594 MS.gene49665:intron 20.0%
!! GATATATAAGTGAAAAGAAA+AGG + chr5.1:27824902-27824921 MS.gene49665:intron 20.0%
!! TATTTAGAAAATAGGATATG+AGG + chr5.1:27824509-27824528 MS.gene49665:intron 20.0%
!! TGAAATAGATATTTAGGAAA+TGG - chr5.1:27825499-27825518 None:intergenic 20.0%
!! TTAAATATCTTCAAATGCTT+GGG + chr5.1:27825636-27825655 MS.gene49665:intron 20.0%
!!! AACTTTTGATATTATGCTAA+GGG + chr5.1:27824831-27824850 MS.gene49665:intron 20.0%
!!! ACGTATTAAACATTAGTTTT+TGG + chr5.1:27824990-27825009 MS.gene49665:intron 20.0%
!!! TAACTTTTGATATTATGCTA+AGG + chr5.1:27824830-27824849 MS.gene49665:intron 20.0%
!!! TCATTTGGTAACATTTTTTT+TGG + chr5.1:27825888-27825907 MS.gene49665:intron 20.0%
!!! TTTTTTGGTAACATTTTTTC+AGG + chr5.1:27825903-27825922 MS.gene49665:intron 20.0%
! AAATCTTTATCCTGCTATAT+TGG + chr5.1:27825138-27825157 MS.gene49665:intron 25.0%
! AAGAAGATCGAATCTAATTA+AGG - chr5.1:27825056-27825075 None:intergenic 25.0%
! ACTAATGTTTAATACGTGTT+AGG - chr5.1:27824987-27825006 None:intergenic 25.0%
! CAATCTAATTCGATTAGATT+AGG + chr5.1:27824726-27824745 MS.gene49665:intron 25.0%
! GCTTATGAGTAATTAAGAAA+TGG - chr5.1:27825334-27825353 None:intergenic 25.0%
! TAAATATCTTCAAATGCTTG+GGG + chr5.1:27825637-27825656 MS.gene49665:intron 25.0%
! TACCAAATGATCTCTTAAAA+GGG - chr5.1:27825878-27825897 None:intergenic 25.0%
! TTACCAAATGATCTCTTAAA+AGG - chr5.1:27825879-27825898 None:intergenic 25.0%
! TTGGTTCAATCAGTTTAAAT+CGG + chr5.1:27824689-27824708 MS.gene49665:intron 25.0%
!! AGCTCACAAAATTTTAAATG+CGG + chr5.1:27825724-27825743 MS.gene49665:intron 25.0%
!! GTTTAAATCGGTTCAATTAA+AGG + chr5.1:27824701-27824720 MS.gene49665:intron 25.0%
!! TTAACCTCTTTTAACATTGA+TGG + chr5.1:27825238-27825257 MS.gene49665:intron 25.0%
!!! AGATTTTAAAGTCAGAGTTA+AGG - chr5.1:27825124-27825143 None:intergenic 25.0%
!!! CTTCAATTGTTTTTAGTTCA+TGG + chr5.1:27825604-27825623 MS.gene49665:intron 25.0%
!!! TAGTTAGCTTTCTTTCTTTT+TGG - chr5.1:27825172-27825191 None:intergenic 25.0%
!!! TTCAATTTTTGCCACTTATT+AGG + chr5.1:27824338-27824357 MS.gene49665:intron 25.0%
ACTGATTGAACCAATTCAAT+CGG - chr5.1:27824683-27824702 None:intergenic 30.0%
AGCAATGTATACCTAATAAG+TGG - chr5.1:27824352-27824371 None:intergenic 30.0%
CTATATTCTAGGTGCAATAT+AGG + chr5.1:27824459-27824478 MS.gene49665:intron 30.0%
GGTATGTGATAAGAATAGAA+AGG - chr5.1:27825035-27825054 None:intergenic 30.0%
GTTAAAAAATCAAACCGAAG+AGG - chr5.1:27824814-27824833 None:intergenic 30.0%
TAAACAAACCTGTTGTGAAT+AGG - chr5.1:27824266-27824285 None:intergenic 30.0%
TATGTGACACTCTATATTCT+AGG + chr5.1:27824448-27824467 MS.gene49665:intron 30.0%
TGTTAGGAAAGGAACATAAT+TGG - chr5.1:27824971-27824990 None:intergenic 30.0%
TGTTTAATACGTGTTAGGAA+AGG - chr5.1:27824982-27825001 None:intergenic 30.0%
TTCAGAGAGAAAAAATGACA+TGG - chr5.1:27825369-27825388 None:intergenic 30.0%
! AAATTTTAAATGCGGAGAAG+TGG + chr5.1:27825732-27825751 MS.gene49665:intron 30.0%
! AATTTTAAATGCGGAGAAGT+GGG + chr5.1:27825733-27825752 MS.gene49665:intron 30.0%
!! TTCCCTTTTAAGAGATCATT+TGG + chr5.1:27825873-27825892 MS.gene49665:intron 30.0%
ACATTGCATAATATATGCGG+GGG - chr5.1:27825781-27825800 None:intergenic 35.0%
AGGACCATCAATGTTAAAAG+AGG - chr5.1:27825245-27825264 None:intergenic 35.0%
AGTAAAAGAAGAGTTCCCAA+TGG + chr5.1:27824233-27824252 MS.gene49665:CDS 35.0%
ATGAATAATATGTTGGTCCC+TGG - chr5.1:27825833-27825852 None:intergenic 35.0%
CATGATGAACAAGTTGTTGT+TGG + chr5.1:27824155-27824174 MS.gene49665:CDS 35.0%
CCAAACAACTGGTCAATTAT+TGG + chr5.1:27824770-27824789 MS.gene49665:intron 35.0%
CCAATAATTGACCAGTTGTT+TGG - chr5.1:27824773-27824792 None:intergenic 35.0%
CCAATTGAACCATAACTCAA+AGG - chr5.1:27824549-27824568 None:intergenic 35.0%
GACAAACAACTACAAGACAT+TGG + chr5.1:27824114-27824133 MS.gene49665:CDS 35.0%
GACATTGCATAATATATGCG+GGG - chr5.1:27825782-27825801 None:intergenic 35.0%
GATTAGATTAGGGTTCAAAC+AGG + chr5.1:27824737-27824756 MS.gene49665:intron 35.0%
GGACATTGCATAATATATGC+GGG - chr5.1:27825783-27825802 None:intergenic 35.0%
GGGACATTGCATAATATATG+CGG - chr5.1:27825784-27825803 None:intergenic 35.0%
TAATTGACCAGTTGTTTGGT+TGG - chr5.1:27824769-27824788 None:intergenic 35.0%
! ACCAATTCAATCGGTTCAAT+CGG - chr5.1:27824674-27824693 None:intergenic 35.0%
! AGTTGTTGTTGGTAAAGACA+AGG + chr5.1:27824166-27824185 MS.gene49665:CDS 35.0%
! ATGGTTCAATTGGTTTATCG+TGG + chr5.1:27824556-27824575 MS.gene49665:intron 35.0%
! ATTTTAAATGCGGAGAAGTG+GGG + chr5.1:27825734-27825753 MS.gene49665:intron 35.0%
! CCTTTGAGTTATGGTTCAAT+TGG + chr5.1:27824546-27824565 MS.gene49665:intron 35.0%
! GTTGTTGTTGGTAAAGACAA+GGG + chr5.1:27824167-27824186 MS.gene49665:CDS 35.0%
!! ACTAGTTGATTTGACCTCTT+CGG + chr5.1:27824797-27824816 MS.gene49665:intron 35.0%
!!! TAACATTTTTTCAGGAGAGC+TGG + chr5.1:27825911-27825930 MS.gene49665:intron 35.0%
AACCTGTTGTGAATAGGATC+TGG - chr5.1:27824260-27824279 None:intergenic 40.0%
AACTACAAGACATTGGTCAG+AGG + chr5.1:27824121-27824140 MS.gene49665:CDS 40.0%
ACCGATTGAACCGATTGAAT+TGG + chr5.1:27824670-27824689 MS.gene49665:intron 40.0%
GATCAAACCAACCAAACAAC+TGG + chr5.1:27824759-27824778 MS.gene49665:intron 40.0%
GGTCAAGTTTCCAATATAGC+AGG - chr5.1:27825151-27825170 None:intergenic 40.0%
TGAAGTCCAATTGTGCATCA+TGG - chr5.1:27824205-27824224 None:intergenic 40.0%
TGAATAGGATCTGGTCCATT+GGG - chr5.1:27824251-27824270 None:intergenic 40.0%
TGTCAACCATGATGCACAAT+TGG + chr5.1:27824196-27824215 MS.gene49665:CDS 40.0%
TTGTCAAGTACCCTAGTAGT+TGG + chr5.1:27825701-27825720 MS.gene49665:intron 40.0%
TTTGTGAGCTCCAACTACTA+GGG - chr5.1:27825714-27825733 None:intergenic 40.0%
! CTCATCTGACCTTTGAGTTA+TGG + chr5.1:27824537-27824556 MS.gene49665:intron 40.0%
! TTTTGTGAGCTCCAACTACT+AGG - chr5.1:27825715-27825734 None:intergenic 40.0%
GACCAGATCCTATTCACAAC+AGG + chr5.1:27824255-27824274 MS.gene49665:CDS 45.0%
GTGAATAGGATCTGGTCCAT+TGG - chr5.1:27824252-27824271 None:intergenic 45.0%
TACCAATTGAGCCAGCTTCA+CGG + chr5.1:27825804-27825823 MS.gene49665:intron 45.0%
TTCAGGAGAGCTGGAAATTC+TGG + chr5.1:27825920-27825939 MS.gene49665:intron 45.0%
! TGAAGCTGGCTCAATTGGTA+GGG - chr5.1:27825804-27825823 None:intergenic 45.0%
! TTAGTTAGTGGTGGAGGAAC+AGG + chr5.1:27824092-27824111 MS.gene49665:CDS 45.0%
ACCAATTGAGCCAGCTTCAC+GGG + chr5.1:27825805-27825824 MS.gene49665:intron 50.0%
GGAAATTCTGGTAGACCTCC+TGG + chr5.1:27825932-27825951 MS.gene49665:CDS 50.0%
TGCATAATATATGCGGGGGC+CGG - chr5.1:27825777-27825796 None:intergenic 50.0%
! GTGAAGCTGGCTCAATTGGT+AGG - chr5.1:27825805-27825824 None:intergenic 50.0%
CATAATATATGCGGGGGCCG+GGG - chr5.1:27825775-27825794 None:intergenic 55.0%
CCAATTGAGCCAGCTTCACG+GGG + chr5.1:27825806-27825825 MS.gene49665:intron 55.0%
GCATAATATATGCGGGGGCC+GGG - chr5.1:27825776-27825795 None:intergenic 55.0%
! ATGCGGAGAAGTGGGGTGTT+CGG + chr5.1:27825741-27825760 MS.gene49665:intron 55.0%
! CCCCGTGAAGCTGGCTCAAT+TGG - chr5.1:27825809-27825828 None:intergenic 60.0%
! TGCGGAGAAGTGGGGTGTTC+GGG + chr5.1:27825742-27825761 MS.gene49665:intron 60.0%
AGCCAGCTTCACGGGGACCA+GGG + chr5.1:27825813-27825832 MS.gene49665:intron 65.0%
GGTGTTCGGGGTTCGAACCC+CGG + chr5.1:27825755-27825774 MS.gene49665:intron 65.0%
! GCGGAGAAGTGGGGTGTTCG+GGG + chr5.1:27825743-27825762 MS.gene49665:intron 65.0%
GAGCCAGCTTCACGGGGACC+AGG + chr5.1:27825812-27825831 MS.gene49665:intron 70.0%
GTCCCTGGTCCCCGTGAAGC+TGG - chr5.1:27825818-27825837 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 27824088 27825964 27824088 ID=MS.gene49665
chr5.1 mRNA 27824088 27825964 27824088 ID=MS.gene49665.t1;Parent=MS.gene49665
chr5.1 exon 27824088 27824276 27824088 ID=MS.gene49665.t1.exon1;Parent=MS.gene49665.t1
chr5.1 CDS 27824088 27824276 27824088 ID=cds.MS.gene49665.t1;Parent=MS.gene49665.t1
chr5.1 exon 27825925 27825964 27825925 ID=MS.gene49665.t1.exon2;Parent=MS.gene49665.t1
chr5.1 CDS 27825925 27825964 27825925 ID=cds.MS.gene49665.t1;Parent=MS.gene49665.t1
Gene Sequence

>MS.gene49665

TAGCTTAGTTAGTGGTGGAGGAACAGGACAAACAACTACAAGACATTGGTCAGAGGAAACACTCAAACATGATGAACAAGTTGTTGTTGGTAAAGACAAGGGTGTTGTTGTCAACCATGATGCACAATTGGACTTCAACTATATGAGTAAAAGAAGAGTTCCCAATGGACCAGATCCTATTCACAACAGGTTTGTTTAGTTTATGTCTATAAACAATTTATATTATAATGTTCATATCTGTCTTTTTAATTTCAATTTTTGCCACTTATTAGGTATACATTGCTTTTAATAAAAAAATAAGAACATATATTTATCTTCTGTTTAGTAATAAAAAAACTTGTGTTTTACTTTGCTAATTAATATGTGACACTCTATATTCTAGGTGCAATATAGGTTATAGTAAGTATAGTATATTATACAATATTTAGAAAATAGGATATGAGGTTTAGCTCATCTGACCTTTGAGTTATGGTTCAATTGGTTTATCGTGGTTGAACTGAATAATGACTAACAAATAAATAGAAAGTAAATAAATATTAAAAGAGTATATAAAAGTAAACATCAAATGAAAAAAATATAAAAACCGATTGAACCGATTGAATTGGTTCAATCAGTTTAAATCGGTTCAATTAAAGGTTCAATCTAATTCGATTAGATTAGGGTTCAAACAGGATCAAACCAACCAAACAACTGGTCAATTATTGGTTCAACTAGTTGATTTGACCTCTTCGGTTTGATTTTTTAACTTTTGATATTATGCTAAGGGCTATATAAGTGCCACTCAAAAAAAAAAAAAAAGAGATAAAAACTAAGGGATATATAAGTGAAAAGAAAAGGAAAACTAGTGCTTTTTTTGTTTGAGTTTAAATTTGATGTGAGACCAATTATGTTCCTTTCCTAACACGTATTAAACATTAGTTTTTGGATTTTTTTTTTTTTTTGGTCCTTTCTATTCTTATCACATACCTTAATTAGATTCGATCTTCTTTCTCAAAGTACAATACACATATTATATGTCTAATCTTTATCTTTTCCTTAACTCTGACTTTAAAATCTTTATCCTGCTATATTGGAAACTTGACCAAAAAGAAAGAAAGCTAACTATGAAAACTGATTATCATGCTTTTAACTGTTACATTGAATTGTTGATTTAACCTCTTTTAACATTGATGGTCCTTTATAATAGATTAACAGATAAAGATATTAAAAAAAGAAGATAAAGTGTATTGTTAAGAACTTTATTCCATTTCTTAATTACTCATAAGCAATTAAAGTTATCCATGTCATTTTTTCTCTCTGAATTAACAGCTTTTCACCTTCTTTTTTTTTGTCTTTCTTTTGATATTGCTTTATTTCTTTTAGCACAAGACACAAGCATGCATGAAGTTACACTTACTATGAACTAAAACCATTTCCTAAATATCTATTTCAAAATTCTAAATAGATCCTTACATTAAATAAATTTGTCACTGAGTCTCATATCTAATCACAAATTATTAACAAATTCAGGATCTAACTTCAATTGTTTTTAGTTCATGGACCTATATATTAAATATCTTCAAATGCTTGGGGATCAATATATATATATATATATATATATATATATATATATTTTGTCAAGTACCCTAGTAGTTGGAGCTCACAAAATTTTAAATGCGGAGAAGTGGGGTGTTCGGGGTTCGAACCCCGGCCCCCGCATATATTATGCAATGTCCCTACCAATTGAGCCAGCTTCACGGGGACCAGGGACCAACATATTATTCATCTATAATAATAACTTTGAATTTCCCTTTTAAGAGATCATTTGGTAACATTTTTTTTGGTAACATTTTTTCAGGAGAGCTGGAAATTCTGGTAGACCTCCTGGTCAAACTTAG

Protein sequence

>MS.gene49665.t1

ULSUWWRNRTNNYKTLVRGNTQTUUTSCCWURQGCCCQPUCTIGLQLYEUKKSSQWTRSYSQQESWKFWUTSWSNL