Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58011.t1 | XP_003609440.1 | 98.3 | 292 | 5 | 0 | 1 | 292 | 1 | 292 | 1.90E-157 | 565.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58011.t1 | Q8H124 | 73.0 | 270 | 65 | 3 | 22 | 289 | 14 | 277 | 3.3e-105 | 382.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58011.t1 | G7JFG1 | 98.3 | 292 | 5 | 0 | 1 | 292 | 1 | 292 | 1.4e-157 | 565.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene58011 | 0.834698 | 2.58E-56 | -1.69E-46 |
| MS.gene049173 | MS.gene58011 | 0.813032 | 3.14E-51 | -1.69E-46 |
| MS.gene049217 | MS.gene58011 | 0.820775 | 5.74E-53 | -1.69E-46 |
| MS.gene049222 | MS.gene58011 | 0.820136 | 8.05E-53 | -1.69E-46 |
| MS.gene049285 | MS.gene58011 | 0.835855 | 1.32E-56 | -1.69E-46 |
| MS.gene049320 | MS.gene58011 | 0.820273 | 7.48E-53 | -1.69E-46 |
| MS.gene049633 | MS.gene58011 | 0.855194 | 7.43E-62 | -1.69E-46 |
| MS.gene049634 | MS.gene58011 | -0.812341 | 4.45E-51 | -1.69E-46 |
| MS.gene050163 | MS.gene58011 | 0.8353 | 1.82E-56 | -1.69E-46 |
| MS.gene050165 | MS.gene58011 | 0.862972 | 3.47E-64 | -1.69E-46 |
| MS.gene050323 | MS.gene58011 | 0.831154 | 1.96E-55 | -1.69E-46 |
| MS.gene050333 | MS.gene58011 | 0.862256 | 5.77E-64 | -1.69E-46 |
| MS.gene050338 | MS.gene58011 | -0.830477 | 2.87E-55 | -1.69E-46 |
| MS.gene050444 | MS.gene58011 | 0.877805 | 4.68E-69 | -1.69E-46 |
| MS.gene050496 | MS.gene58011 | 0.808247 | 3.40E-50 | -1.69E-46 |
| MS.gene050504 | MS.gene58011 | 0.818186 | 2.24E-52 | -1.69E-46 |
| MS.gene050518 | MS.gene58011 | 0.807461 | 5.00E-50 | -1.69E-46 |
| MS.gene050612 | MS.gene58011 | 0.86177 | 8.13E-64 | -1.69E-46 |
| MS.gene050675 | MS.gene58011 | 0.829192 | 5.91E-55 | -1.69E-46 |
| MS.gene050772 | MS.gene58011 | 0.809226 | 2.10E-50 | -1.69E-46 |
| MS.gene05079 | MS.gene58011 | -0.848598 | 5.53E-60 | -1.69E-46 |
| MS.gene050809 | MS.gene58011 | -0.817718 | 2.85E-52 | -1.69E-46 |
| MS.gene05080 | MS.gene58011 | 0.833106 | 6.45E-56 | -1.69E-46 |
| MS.gene050868 | MS.gene58011 | 0.818034 | 2.42E-52 | -1.69E-46 |
| MS.gene050871 | MS.gene58011 | 0.813221 | 2.86E-51 | -1.69E-46 |
| MS.gene050876 | MS.gene58011 | 0.824238 | 9.00E-54 | -1.69E-46 |
| MS.gene050904 | MS.gene58011 | 0.834497 | 2.90E-56 | -1.69E-46 |
| MS.gene051138 | MS.gene58011 | -0.837396 | 5.33E-57 | -1.69E-46 |
| MS.gene051228 | MS.gene58011 | 0.851346 | 9.42E-61 | -1.69E-46 |
| MS.gene051317 | MS.gene58011 | 0.840157 | 1.03E-57 | -1.69E-46 |
| MS.gene051434 | MS.gene58011 | 0.823158 | 1.61E-53 | -1.69E-46 |
| MS.gene051617 | MS.gene58011 | 0.85527 | 7.06E-62 | -1.69E-46 |
| MS.gene051651 | MS.gene58011 | -0.827993 | 1.15E-54 | -1.69E-46 |
| MS.gene051810 | MS.gene58011 | 0.841735 | 3.97E-58 | -1.69E-46 |
| MS.gene051858 | MS.gene58011 | 0.807963 | 3.91E-50 | -1.69E-46 |
| MS.gene051874 | MS.gene58011 | 0.816474 | 5.43E-52 | -1.69E-46 |
| MS.gene051949 | MS.gene58011 | 0.853305 | 2.61E-61 | -1.69E-46 |
| MS.gene052129 | MS.gene58011 | -0.852553 | 4.28E-61 | -1.69E-46 |
| MS.gene052870 | MS.gene58011 | 0.87514 | 3.89E-68 | -1.69E-46 |
| MS.gene052872 | MS.gene58011 | 0.889672 | 1.96E-73 | -1.69E-46 |
| MS.gene052916 | MS.gene58011 | 0.805143 | 1.54E-49 | -1.69E-46 |
| MS.gene052966 | MS.gene58011 | 0.809051 | 2.29E-50 | -1.69E-46 |
| MS.gene053284 | MS.gene58011 | 0.832434 | 9.47E-56 | -1.69E-46 |
| MS.gene053285 | MS.gene58011 | 0.821886 | 3.18E-53 | -1.69E-46 |
| MS.gene053384 | MS.gene58011 | 0.804969 | 1.67E-49 | -1.69E-46 |
| MS.gene053479 | MS.gene58011 | 0.808658 | 2.78E-50 | -1.69E-46 |
| MS.gene053824 | MS.gene58011 | 0.837851 | 4.07E-57 | -1.69E-46 |
| MS.gene053916 | MS.gene58011 | 0.82274 | 2.02E-53 | -1.69E-46 |
| MS.gene054642 | MS.gene58011 | 0.826249 | 3.01E-54 | -1.69E-46 |
| MS.gene05494 | MS.gene58011 | 0.80713 | 5.87E-50 | -1.69E-46 |
| MS.gene054954 | MS.gene58011 | 0.823156 | 1.61E-53 | -1.69E-46 |
| MS.gene055206 | MS.gene58011 | 0.8079 | 4.03E-50 | -1.69E-46 |
| MS.gene055224 | MS.gene58011 | 0.806032 | 1.00E-49 | -1.69E-46 |
| MS.gene055362 | MS.gene58011 | 0.801068 | 1.07E-48 | -1.69E-46 |
| MS.gene055502 | MS.gene58011 | 0.825444 | 4.67E-54 | -1.69E-46 |
| MS.gene055577 | MS.gene58011 | 0.846841 | 1.69E-59 | -1.69E-46 |
| MS.gene055638 | MS.gene58011 | 0.816195 | 6.27E-52 | -1.69E-46 |
| MS.gene055748 | MS.gene58011 | 0.873086 | 1.93E-67 | -1.69E-46 |
| MS.gene055750 | MS.gene58011 | 0.843443 | 1.40E-58 | -1.69E-46 |
| MS.gene055751 | MS.gene58011 | 0.887988 | 8.79E-73 | -1.69E-46 |
| MS.gene055776 | MS.gene58011 | 0.814834 | 1.26E-51 | -1.69E-46 |
| MS.gene05589 | MS.gene58011 | 0.84145 | 4.72E-58 | -1.69E-46 |
| MS.gene055941 | MS.gene58011 | 0.81735 | 3.45E-52 | -1.69E-46 |
| MS.gene055942 | MS.gene58011 | -0.803891 | 2.81E-49 | -1.69E-46 |
| MS.gene056283 | MS.gene58011 | 0.842154 | 3.08E-58 | -1.69E-46 |
| MS.gene056387 | MS.gene58011 | 0.806496 | 8.00E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene58011 | 0.82758 | 1.45E-54 | -1.69E-46 |
| MS.gene056549 | MS.gene58011 | 0.827059 | 1.93E-54 | -1.69E-46 |
| MS.gene056657 | MS.gene58011 | 0.822862 | 1.89E-53 | -1.69E-46 |
| MS.gene056692 | MS.gene58011 | 0.831095 | 2.03E-55 | -1.69E-46 |
| MS.gene056856 | MS.gene58011 | -0.856043 | 4.20E-62 | -1.69E-46 |
| MS.gene056857 | MS.gene58011 | -0.829957 | 3.85E-55 | -1.69E-46 |
| MS.gene056903 | MS.gene58011 | 0.845795 | 3.25E-59 | -1.69E-46 |
| MS.gene057076 | MS.gene58011 | 0.801832 | 7.48E-49 | -1.69E-46 |
| MS.gene057246 | MS.gene58011 | 0.832152 | 1.11E-55 | -1.69E-46 |
| MS.gene057831 | MS.gene58011 | 0.803952 | 2.73E-49 | -1.69E-46 |
| MS.gene057857 | MS.gene58011 | 0.801786 | 7.64E-49 | -1.69E-46 |
| MS.gene058334 | MS.gene58011 | -0.80681 | 6.86E-50 | -1.69E-46 |
| MS.gene058335 | MS.gene58011 | 0.801286 | 9.67E-49 | -1.69E-46 |
| MS.gene058419 | MS.gene58011 | 0.828858 | 7.12E-55 | -1.69E-46 |
| MS.gene058603 | MS.gene58011 | -0.81369 | 2.25E-51 | -1.69E-46 |
| MS.gene058723 | MS.gene58011 | 0.835025 | 2.13E-56 | -1.69E-46 |
| MS.gene058773 | MS.gene58011 | 0.841334 | 5.06E-58 | -1.69E-46 |
| MS.gene058838 | MS.gene58011 | 0.825666 | 4.14E-54 | -1.69E-46 |
| MS.gene059022 | MS.gene58011 | 0.82207 | 2.88E-53 | -1.69E-46 |
| MS.gene059023 | MS.gene58011 | 0.849704 | 2.73E-60 | -1.69E-46 |
| MS.gene059029 | MS.gene58011 | 0.810808 | 9.59E-51 | -1.69E-46 |
| MS.gene059079 | MS.gene58011 | 0.89217 | 2.03E-74 | -1.69E-46 |
| MS.gene05912 | MS.gene58011 | 0.818157 | 2.27E-52 | -1.69E-46 |
| MS.gene05913 | MS.gene58011 | 0.827313 | 1.68E-54 | -1.69E-46 |
| MS.gene05934 | MS.gene58011 | 0.802579 | 5.25E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene58011 | 0.843014 | 1.82E-58 | -1.69E-46 |
| MS.gene059865 | MS.gene58011 | 0.836269 | 1.03E-56 | -1.69E-46 |
| MS.gene060703 | MS.gene58011 | 0.829995 | 3.77E-55 | -1.69E-46 |
| MS.gene060762 | MS.gene58011 | 0.865307 | 6.49E-65 | -1.69E-46 |
| MS.gene060818 | MS.gene58011 | 0.80075 | 1.24E-48 | -1.69E-46 |
| MS.gene060823 | MS.gene58011 | -0.827126 | 1.86E-54 | -1.69E-46 |
| MS.gene060832 | MS.gene58011 | 0.811939 | 5.45E-51 | -1.69E-46 |
| MS.gene060981 | MS.gene58011 | 0.81768 | 2.91E-52 | -1.69E-46 |
| MS.gene060982 | MS.gene58011 | 0.849936 | 2.35E-60 | -1.69E-46 |
| MS.gene061044 | MS.gene58011 | -0.80442 | 2.18E-49 | -1.69E-46 |
| MS.gene06111 | MS.gene58011 | 0.800371 | 1.49E-48 | -1.69E-46 |
| MS.gene061150 | MS.gene58011 | 0.818944 | 1.50E-52 | -1.69E-46 |
| MS.gene061231 | MS.gene58011 | 0.811927 | 5.48E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58011.t1 | MTR_4g115730 | 98.288 | 292 | 5 | 0 | 1 | 292 | 1 | 292 | 0.0 | 589 |
| MS.gene58011.t1 | MTR_4g115730 | 100.000 | 252 | 0 | 0 | 41 | 292 | 1 | 252 | 0.0 | 514 |
| MS.gene58011.t1 | MTR_4g068970 | 35.185 | 270 | 140 | 9 | 42 | 289 | 70 | 326 | 2.56e-32 | 125 |
| MS.gene58011.t1 | MTR_4g021815 | 27.715 | 267 | 147 | 11 | 56 | 292 | 4 | 254 | 3.88e-13 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58011.t1 | AT2G34460 | 72.963 | 270 | 65 | 3 | 22 | 289 | 14 | 277 | 2.66e-141 | 399 |
| MS.gene58011.t1 | AT3G18890 | 33.453 | 278 | 149 | 11 | 41 | 292 | 62 | 329 | 4.52e-30 | 119 |
| MS.gene58011.t1 | AT2G37660 | 30.952 | 168 | 99 | 7 | 135 | 292 | 164 | 324 | 1.19e-12 | 67.8 |
| MS.gene58011.t1 | AT4G31530 | 31.746 | 315 | 158 | 17 | 8 | 286 | 25 | 318 | 8.19e-11 | 62.4 |
Find 79 sgRNAs with CRISPR-Local
Find 152 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTATGCAGGTTGACAATTT+TGG | 0.186826 | 8.3:-10389206 | MS.gene58011:intron |
| AGCGGCTCCATTGACTAAAA+TGG | 0.269340 | 8.3:+10389121 | None:intergenic |
| CACCTTAGTGGCAAAATTAC+AGG | 0.310873 | 8.3:-10389048 | MS.gene58011:CDS |
| TGCAACTTGATCTCTGGATA+TGG | 0.317263 | 8.3:+10388799 | None:intergenic |
| CCACAGGCTTTCGGCCAGGC+TGG | 0.324155 | 8.3:-10390005 | MS.gene58011:CDS |
| GTTGAGGAAGGGAAGAGATT+TGG | 0.346298 | 8.3:+10390785 | None:intergenic |
| CGCGCATACCATGATCTCTT+TGG | 0.346559 | 8.3:-10388695 | MS.gene58011:CDS |
| TAGTTTGTGCCACAGGCTTT+CGG | 0.360254 | 8.3:-10390014 | MS.gene58011:CDS |
| CAGATATTATCGCTGAACTA+TGG | 0.361240 | 8.3:+10388670 | None:intergenic |
| GAAAATCATATCAGGAAATC+TGG | 0.374335 | 8.3:-10389023 | MS.gene58011:CDS |
| GATAAGCTAGCTGAAGCAAT+TGG | 0.379075 | 8.3:-10390054 | MS.gene58011:CDS |
| TGGATATGGAACCTTCATAA+AGG | 0.390225 | 8.3:+10388813 | None:intergenic |
| TACAGGCAGAAAATCATATC+AGG | 0.402158 | 8.3:-10389031 | MS.gene58011:CDS |
| TCCTACTGGAAATGTTGTTA+TGG | 0.413074 | 8.3:-10388955 | MS.gene58011:CDS |
| CACAGGCTTTCGGCCAGGCT+GGG | 0.417213 | 8.3:-10390004 | MS.gene58011:CDS |
| AGGAAGAGGGAGCGGTGAAT+TGG | 0.419663 | 8.3:+10390814 | None:intergenic |
| ATAAGCTAGCTGAAGCAATT+GGG | 0.423531 | 8.3:-10390053 | MS.gene58011:CDS |
| AGATTTGGTTCTGAAGGAAG+AGG | 0.436139 | 8.3:+10390800 | None:intergenic |
| GTAGGGTTTCTTGCAATAAG+AGG | 0.436214 | 8.3:+10390864 | None:intergenic |
| TGTGCCACAGGCTTTCGGCC+AGG | 0.448901 | 8.3:-10390009 | MS.gene58011:CDS |
| TTAGTCAATGGAGCCGCTAT+GGG | 0.459534 | 8.3:-10389116 | MS.gene58011:CDS |
| TTTAGTCAATGGAGCCGCTA+TGG | 0.462769 | 8.3:-10389117 | MS.gene58011:CDS |
| AAACTTACAAATTGAAGAGA+TGG | 0.465083 | 8.3:+10390495 | None:intergenic |
| TACTTCTGCAACTTGATCTC+TGG | 0.466748 | 8.3:+10388793 | None:intergenic |
| GCTGGAGCTACTGGTAGTAC+TGG | 0.469225 | 8.3:-10390612 | MS.gene58011:CDS |
| GATGGGAAAGTGTGGAATGT+AGG | 0.469287 | 8.3:+10390846 | None:intergenic |
| GGGAAGAGATTTGGTTCTGA+AGG | 0.477299 | 8.3:+10390794 | None:intergenic |
| GGATTTGCTTGCCCCATGGA+AGG | 0.484251 | 8.3:-10389983 | MS.gene58011:intron |
| AGATCATGGTATGCGCGCTT+AGG | 0.484675 | 8.3:+10388701 | None:intergenic |
| ATGGGAAAGTGTGGAATGTA+GGG | 0.487437 | 8.3:+10390847 | None:intergenic |
| GGATATGGAACCTTCATAAA+GGG | 0.488764 | 8.3:+10388814 | None:intergenic |
| TAAAGCTGGGGTTAGAGACT+TGG | 0.490510 | 8.3:-10390547 | MS.gene58011:CDS |
| CTATGGAACCAAAGAGATCA+TGG | 0.496271 | 8.3:+10388687 | None:intergenic |
| GAAGCAGTAGTTTGTGCCAC+AGG | 0.496380 | 8.3:-10390021 | MS.gene58011:CDS |
| TACAAATTGAAGAGATGGGT+TGG | 0.504301 | 8.3:+10390500 | None:intergenic |
| GCTGGGATTTGCTTGCCCCA+TGG | 0.510222 | 8.3:-10389987 | MS.gene58011:CDS |
| GTGGAACAGTTACTTGCAAA+GGG | 0.511281 | 8.3:-10390579 | MS.gene58011:CDS |
| TGTCAGGACACCCTTTATGA+AGG | 0.512820 | 8.3:-10388824 | MS.gene58011:intron |
| AAAATTAACAAACCTTCCAT+GGG | 0.515977 | 8.3:+10389971 | None:intergenic |
| CGTGGAACAGTTACTTGCAA+AGG | 0.522560 | 8.3:-10390580 | MS.gene58011:CDS |
| CTTTCCTGCATGCTTCAACA+AGG | 0.527070 | 8.3:+10389174 | None:intergenic |
| ACAACTTTGTAAGATGCCTC+AGG | 0.530129 | 8.3:+10388749 | None:intergenic |
| TATGCGCGCTTAGGAGCATC+AGG | 0.530362 | 8.3:+10388710 | None:intergenic |
| TAAACCTTGTTGAAGCATGC+AGG | 0.536699 | 8.3:-10389178 | MS.gene58011:CDS |
| GTGAATTGGCGAGTGTGGGA+TGG | 0.545645 | 8.3:+10390828 | None:intergenic |
| GAGCGGTGAATTGGCGAGTG+TGG | 0.546264 | 8.3:+10390823 | None:intergenic |
| TGAATTGGCGAGTGTGGGAT+GGG | 0.547232 | 8.3:+10390829 | None:intergenic |
| AACTTACAAATTGAAGAGAT+GGG | 0.550774 | 8.3:+10390496 | None:intergenic |
| GGGGCAAGCAAATCCCAGCC+TGG | 0.552735 | 8.3:+10389991 | None:intergenic |
| TACCTTTGCGAAGTTGAGGA+AGG | 0.555784 | 8.3:+10390773 | None:intergenic |
| AGCGGTGAATTGGCGAGTGT+GGG | 0.558367 | 8.3:+10390824 | None:intergenic |
| GTGTTACCTTTGCGAAGTTG+AGG | 0.563021 | 8.3:+10390769 | None:intergenic |
| CTGGAGCTACTGGTAGTACT+GGG | 0.565656 | 8.3:-10390611 | MS.gene58011:CDS |
| TGAGGCATCTTACAAAGTTG+TGG | 0.567286 | 8.3:-10388747 | MS.gene58011:CDS |
| TATGGGGCAGCTACTCAATC+CGG | 0.569169 | 8.3:-10389099 | MS.gene58011:CDS |
| AGAAGTAGCTGTGGAATCGT+TGG | 0.570182 | 8.3:-10388777 | MS.gene58011:CDS |
| GGTTCTGAAGGAAGAGGGAG+CGG | 0.573272 | 8.3:+10390806 | None:intergenic |
| GATTTGGTTCTGAAGGAAGA+GGG | 0.577302 | 8.3:+10390801 | None:intergenic |
| ACCTTTGCGAAGTTGAGGAA+GGG | 0.578834 | 8.3:+10390774 | None:intergenic |
| CCAGCCTGGCCGAAAGCCTG+TGG | 0.586862 | 8.3:+10390005 | None:intergenic |
| ATAACAACATTTCCAGTAGG+AGG | 0.590217 | 8.3:+10388957 | None:intergenic |
| ACGTTGAGAAAGATGTAAGC+CGG | 0.592173 | 8.3:+10389080 | None:intergenic |
| TCCCTTCCTCAACTTCGCAA+AGG | 0.592473 | 8.3:-10390775 | MS.gene58011:intron |
| TAAGCTAGCTGAAGCAATTG+GGG | 0.593302 | 8.3:-10390052 | MS.gene58011:CDS |
| TGAAATCACTCAGCAAGCAG+TGG | 0.616011 | 8.3:-10390670 | MS.gene58011:CDS |
| GAGTGTGGGATGGGAAAGTG+TGG | 0.617968 | 8.3:+10390838 | None:intergenic |
| GTTAAAGTTGATGTCACAGA+GGG | 0.630073 | 8.3:-10390081 | MS.gene58011:CDS |
| TCAAGTTGCAGAAGTAGCTG+TGG | 0.632803 | 8.3:-10388786 | MS.gene58011:CDS |
| ATTGAGTAGCTGCCCCATAG+CGG | 0.638328 | 8.3:+10389103 | None:intergenic |
| AATTACACAATCATAAGACC+TGG | 0.643894 | 8.3:-10388996 | MS.gene58011:CDS |
| TAGTCAATGGAGCCGCTATG+GGG | 0.647798 | 8.3:-10389115 | MS.gene58011:CDS |
| TACACAATCATAAGACCTGG+AGG | 0.652611 | 8.3:-10388993 | MS.gene58011:CDS |
| TCCATAACAACATTTCCAGT+AGG | 0.656385 | 8.3:+10388954 | None:intergenic |
| GGAATCGTTGGCTTATCCTG+AGG | 0.676950 | 8.3:-10388765 | MS.gene58011:CDS |
| TAGTACTGGGAAAAGAATCG+TGG | 0.683015 | 8.3:-10390598 | MS.gene58011:CDS |
| ACACAATCATAAGACCTGGA+GGG | 0.693729 | 8.3:-10388992 | MS.gene58011:CDS |
| AAATGTTGTTATGGAGCCAG+AGG | 0.716025 | 8.3:-10388946 | MS.gene58011:intron |
| AAATTAACAAACCTTCCATG+GGG | 0.740909 | 8.3:+10389972 | None:intergenic |
| GGTTAAAGTTGATGTCACAG+AGG | 0.749642 | 8.3:-10390082 | MS.gene58011:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTATTTTTATGTTAGTA+TGG | - | chr8.3:10389655-10389674 | MS.gene58011:intron | 10.0% |
| !! | TATAACAAAATATTGTATAC+TGG | + | chr8.3:10390311-10390330 | None:intergenic | 15.0% |
| !!! | ACAATATTTTGTTATAACAA+AGG | - | chr8.3:10390315-10390334 | MS.gene58011:intron | 15.0% |
| !!! | CAATATTTTGTTATAACAAA+GGG | - | chr8.3:10390316-10390335 | MS.gene58011:intron | 15.0% |
| !!! | TTTTTTTTCTTTTTGTTAGA+TGG | - | chr8.3:10388849-10388868 | MS.gene58011:intron | 15.0% |
| !! | TTTAAATCTAAACCAAGTTA+AGG | + | chr8.3:10389719-10389738 | None:intergenic | 20.0% |
| !!! | AATTTAATTTTCTATGTGTC+AGG | - | chr8.3:10390709-10390728 | MS.gene58011:intron | 20.0% |
| !!! | ATAGTCCAATTTAAGATTAT+TGG | - | chr8.3:10389104-10389123 | MS.gene58011:CDS | 20.0% |
| !!! | CTCAATTTTTTATATGTTTG+TGG | - | chr8.3:10390672-10390691 | MS.gene58011:CDS | 20.0% |
| !!! | TAGTCCAATTTAAGATTATT+GGG | - | chr8.3:10389105-10389124 | MS.gene58011:CDS | 20.0% |
| !!! | TATAATCTGATGCTATTTAA+AGG | + | chr8.3:10390202-10390221 | None:intergenic | 20.0% |
| ! | AAAAAATGCTAGTATTAACG+CGG | + | chr8.3:10388834-10388853 | None:intergenic | 25.0% |
| ! | AAAAATTAACAAACCTTCCA+TGG | + | chr8.3:10389582-10389601 | None:intergenic | 25.0% |
| ! | AAAATTAACAAACCTTCCAT+GGG | + | chr8.3:10389581-10389600 | None:intergenic | 25.0% |
| ! | AAACTTACAAATTGAAGAGA+TGG | + | chr8.3:10389057-10389076 | None:intergenic | 25.0% |
| ! | AACTTACAAATTGAAGAGAT+GGG | + | chr8.3:10389056-10389075 | None:intergenic | 25.0% |
| ! | ACTGTAACACTAATATAATC+AGG | - | chr8.3:10389928-10389947 | MS.gene58011:intron | 25.0% |
| ! | ATGAATCAAAACACAATTGT+TGG | - | chr8.3:10390256-10390275 | MS.gene58011:intron | 25.0% |
| ! | ATGGTAGTAGAATATTAGAA+TGG | - | chr8.3:10389612-10389631 | MS.gene58011:intron | 25.0% |
| ! | CAAATAAACCAATTCAAACA+GGG | - | chr8.3:10389381-10389400 | MS.gene58011:intron | 25.0% |
| ! | TCAAATAAACCAATTCAAAC+AGG | - | chr8.3:10389380-10389399 | MS.gene58011:intron | 25.0% |
| ! | TTAGATTCTTCACTAGTAAA+GGG | - | chr8.3:10390133-10390152 | MS.gene58011:intron | 25.0% |
| ! | TTTAGATTCTTCACTAGTAA+AGG | - | chr8.3:10390132-10390151 | MS.gene58011:intron | 25.0% |
| !! | GGACTATTTTGCAATATAAT+GGG | + | chr8.3:10389091-10389110 | None:intergenic | 25.0% |
| !! | TGGACTATTTTGCAATATAA+TGG | + | chr8.3:10389092-10389111 | None:intergenic | 25.0% |
| !! | TTTTCTTTTTGTTAGATGGA+AGG | - | chr8.3:10388853-10388872 | MS.gene58011:intron | 25.0% |
| !!! | AAAATGATCTCTAGAACTTA+CGG | - | chr8.3:10389744-10389763 | MS.gene58011:intron | 25.0% |
| !!! | ACAATTGTGTTTTGATTCAT+TGG | + | chr8.3:10390256-10390275 | None:intergenic | 25.0% |
| !!! | ATAAGTTCCATTTTAGTCAA+TGG | - | chr8.3:10390421-10390440 | MS.gene58011:intron | 25.0% |
| AAATTAACAAACCTTCCATG+GGG | + | chr8.3:10389580-10389599 | None:intergenic | 30.0% | |
| AACAAAGGGATATATTATGC+AGG | - | chr8.3:10390330-10390349 | MS.gene58011:intron | 30.0% | |
| AATTACACAATCATAAGACC+TGG | - | chr8.3:10390553-10390572 | MS.gene58011:CDS | 30.0% | |
| ACTAGTGAAGAATCTAAACT+TGG | + | chr8.3:10390130-10390149 | None:intergenic | 30.0% | |
| ACTCTATCTAATATCCAGAA+TGG | + | chr8.3:10390018-10390037 | None:intergenic | 30.0% | |
| AGTAGGCTACATTATATACA+TGG | - | chr8.3:10389852-10389871 | MS.gene58011:intron | 30.0% | |
| ATCAAAACACAATTGTTGGT+TGG | - | chr8.3:10390260-10390279 | MS.gene58011:intron | 30.0% | |
| CAGACCCAATAATCTTAAAT+TGG | + | chr8.3:10389112-10389131 | None:intergenic | 30.0% | |
| GAAAATCATATCAGGAAATC+TGG | - | chr8.3:10390526-10390545 | MS.gene58011:CDS | 30.0% | |
| GAATGACATGATGTGTAATT+TGG | - | chr8.3:10389420-10389439 | MS.gene58011:intron | 30.0% | |
| GTACAAGTTCTGTAAAAGTT+TGG | - | chr8.3:10389813-10389832 | MS.gene58011:intron | 30.0% | |
| TAGAACTTACGGTGATTATT+GGG | - | chr8.3:10389755-10389774 | MS.gene58011:intron | 30.0% | |
| TATAAACAATTTGCCCTGTT+TGG | - | chr8.3:10389132-10389151 | MS.gene58011:CDS | 30.0% | |
| ! | AAGAAGAAAGTTTTTGTTGC+TGG | - | chr8.3:10388919-10388938 | MS.gene58011:intron | 30.0% |
| ! | ATTAAGTTGTTGATCCAAAC+AGG | + | chr8.3:10389149-10389168 | None:intergenic | 30.0% |
| ! | TACATCTTTCTCAACGTTTT+CGG | - | chr8.3:10390475-10390494 | MS.gene58011:intron | 30.0% |
| ! | TCAAAACACAATTGTTGGTT+GGG | - | chr8.3:10390261-10390280 | MS.gene58011:intron | 30.0% |
| ! | TTAAGTTGTTGATCCAAACA+GGG | + | chr8.3:10389148-10389167 | None:intergenic | 30.0% |
| !! | ATTATGCAGGTTGACAATTT+TGG | - | chr8.3:10390343-10390362 | MS.gene58011:intron | 30.0% |
| !!! | GTGTTTGATACTTTTGAAGT+AGG | - | chr8.3:10389835-10389854 | MS.gene58011:intron | 30.0% |
| AAGCTATCATGTAGAGCTTA+TGG | - | chr8.3:10389278-10389297 | MS.gene58011:intron | 35.0% | |
| ATAACAACATTTCCAGTAGG+AGG | + | chr8.3:10390595-10390614 | None:intergenic | 35.0% | |
| ATAAGCTAGCTGAAGCAATT+GGG | - | chr8.3:10389496-10389515 | MS.gene58011:intron | 35.0% | |
| ATATGTGTGAGTTCATATGC+AGG | - | chr8.3:10389446-10389465 | MS.gene58011:intron | 35.0% | |
| CTAGAACTTACGGTGATTAT+TGG | - | chr8.3:10389754-10389773 | MS.gene58011:intron | 35.0% | |
| CTGGATATTAGATAGAGTTG+AGG | - | chr8.3:10390020-10390039 | MS.gene58011:CDS | 35.0% | |
| GAAATTTAAGCAAACACCTC+TGG | + | chr8.3:10390622-10390641 | None:intergenic | 35.0% | |
| GGATATGGAACCTTCATAAA+GGG | + | chr8.3:10390738-10390757 | None:intergenic | 35.0% | |
| GGTTACTAAAACAAGAACCA+CGG | + | chr8.3:10389991-10390010 | None:intergenic | 35.0% | |
| TACAAATTGAAGAGATGGGT+TGG | + | chr8.3:10389052-10389071 | None:intergenic | 35.0% | |
| TACAGGCAGAAAATCATATC+AGG | - | chr8.3:10390518-10390537 | MS.gene58011:CDS | 35.0% | |
| TCCATAACAACATTTCCAGT+AGG | + | chr8.3:10390598-10390617 | None:intergenic | 35.0% | |
| TCCTACTGGAAATGTTGTTA+TGG | - | chr8.3:10390594-10390613 | MS.gene58011:CDS | 35.0% | |
| TGGATATGGAACCTTCATAA+AGG | + | chr8.3:10390739-10390758 | None:intergenic | 35.0% | |
| ! | TGGGTGCAACATATTTTCAT+TGG | - | chr8.3:10389774-10389793 | MS.gene58011:intron | 35.0% |
| !! | GTTAAAGTTGATGTCACAGA+GGG | - | chr8.3:10389468-10389487 | MS.gene58011:intron | 35.0% |
| AAATGTTGTTATGGAGCCAG+AGG | - | chr8.3:10390603-10390622 | MS.gene58011:CDS | 40.0% | |
| ACAACTTTGTAAGATGCCTC+AGG | + | chr8.3:10390803-10390822 | None:intergenic | 40.0% | |
| ACACAATCATAAGACCTGGA+GGG | - | chr8.3:10390557-10390576 | MS.gene58011:CDS | 40.0% | |
| ACAGTTTAACACGCAGAAAC+CGG | + | chr8.3:10389913-10389932 | None:intergenic | 40.0% | |
| ACGTTGAGAAAGATGTAAGC+CGG | + | chr8.3:10390472-10390491 | None:intergenic | 40.0% | |
| CACCTTAGTGGCAAAATTAC+AGG | - | chr8.3:10390501-10390520 | MS.gene58011:intron | 40.0% | |
| CTATGGAACCAAAGAGATCA+TGG | + | chr8.3:10390865-10390884 | None:intergenic | 40.0% | |
| GATAAGCTAGCTGAAGCAAT+TGG | - | chr8.3:10389495-10389514 | MS.gene58011:intron | 40.0% | |
| GTAGGGTTTCTTGCAATAAG+AGG | + | chr8.3:10388688-10388707 | None:intergenic | 40.0% | |
| GTGGAACAGTTACTTGCAAA+GGG | - | chr8.3:10388970-10388989 | MS.gene58011:CDS | 40.0% | |
| TAAACCTTGTTGAAGCATGC+AGG | - | chr8.3:10390371-10390390 | MS.gene58011:intron | 40.0% | |
| TAAGCTAGCTGAAGCAATTG+GGG | - | chr8.3:10389497-10389516 | MS.gene58011:intron | 40.0% | |
| TACACAATCATAAGACCTGG+AGG | - | chr8.3:10390556-10390575 | MS.gene58011:CDS | 40.0% | |
| TACTTCTGCAACTTGATCTC+TGG | + | chr8.3:10390759-10390778 | None:intergenic | 40.0% | |
| TCTAATATCCAGAATGGCGT+TGG | + | chr8.3:10390012-10390031 | None:intergenic | 40.0% | |
| TGCAACTTGATCTCTGGATA+TGG | + | chr8.3:10390753-10390772 | None:intergenic | 40.0% | |
| TTGAAAAACGATCCTCCTAC+TGG | - | chr8.3:10390580-10390599 | MS.gene58011:CDS | 40.0% | |
| TTTAGTAACCAACGCCATTC+TGG | - | chr8.3:10390001-10390020 | MS.gene58011:CDS | 40.0% | |
| ! | ATGGGAAAGTGTGGAATGTA+GGG | + | chr8.3:10388705-10388724 | None:intergenic | 40.0% |
| ! | GTTAATGAGTGGCCTTAACT+TGG | - | chr8.3:10389704-10389723 | MS.gene58011:intron | 40.0% |
| ! | TAGTACTGGGAAAAGAATCG+TGG | - | chr8.3:10388951-10388970 | MS.gene58011:CDS | 40.0% |
| ! | TGAGGCATCTTACAAAGTTG+TGG | - | chr8.3:10390802-10390821 | MS.gene58011:CDS | 40.0% |
| ! | TGCCTGTAATTTTGCCACTA+AGG | + | chr8.3:10390506-10390525 | None:intergenic | 40.0% |
| ! | TTGATTCCACATTTTCCCTC+CGG | - | chr8.3:10389891-10389910 | MS.gene58011:intron | 40.0% |
| !! | AGATTTGGTTCTGAAGGAAG+AGG | + | chr8.3:10388752-10388771 | None:intergenic | 40.0% |
| !! | CAAGTACTTCTGAGACTCTA+TGG | + | chr8.3:10389345-10389364 | None:intergenic | 40.0% |
| !! | GATTTGGTTCTGAAGGAAGA+GGG | + | chr8.3:10388751-10388770 | None:intergenic | 40.0% |
| !! | GGTTAAAGTTGATGTCACAG+AGG | - | chr8.3:10389467-10389486 | MS.gene58011:intron | 40.0% |
| !!! | AAGGGTTTTGCTGTTAAAGC+TGG | - | chr8.3:10388988-10389007 | MS.gene58011:CDS | 40.0% |
| !!! | AGGGTTTTGCTGTTAAAGCT+GGG | - | chr8.3:10388989-10389008 | MS.gene58011:CDS | 40.0% |
| ACACACAGCCCTGTTTGAAT+TGG | + | chr8.3:10389392-10389411 | None:intergenic | 45.0% | |
| ACCTTTGCGAAGTTGAGGAA+GGG | + | chr8.3:10388778-10388797 | None:intergenic | 45.0% | |
| AGAAGTAGCTGTGGAATCGT+TGG | - | chr8.3:10390772-10390791 | MS.gene58011:intron | 45.0% | |
| CGTGGAACAGTTACTTGCAA+AGG | - | chr8.3:10388969-10388988 | MS.gene58011:CDS | 45.0% | |
| CTTTCCTGCATGCTTCAACA+AGG | + | chr8.3:10390378-10390397 | None:intergenic | 45.0% | |
| GATAGCTTTCATTCATGCCG+TGG | - | chr8.3:10389971-10389990 | MS.gene58011:intron | 45.0% | |
| GTGTTACCTTTGCGAAGTTG+AGG | + | chr8.3:10388783-10388802 | None:intergenic | 45.0% | |
| GTTGAGGAAGGGAAGAGATT+TGG | + | chr8.3:10388767-10388786 | None:intergenic | 45.0% | |
| TAAAGCTGGGGTTAGAGACT+TGG | - | chr8.3:10389002-10389021 | MS.gene58011:CDS | 45.0% | |
| TAAGGCCACTCATTAACCAG+TGG | + | chr8.3:10389701-10389720 | None:intergenic | 45.0% | |
| TACCTTTGCGAAGTTGAGGA+AGG | + | chr8.3:10388779-10388798 | None:intergenic | 45.0% | |
| TGAAATCACTCAGCAAGCAG+TGG | - | chr8.3:10388879-10388898 | MS.gene58011:intron | 45.0% | |
| TGTCAGGACACCCTTTATGA+AGG | - | chr8.3:10390725-10390744 | MS.gene58011:intron | 45.0% | |
| TTAGTCAATGGAGCCGCTAT+GGG | - | chr8.3:10390433-10390452 | MS.gene58011:intron | 45.0% | |
| TTTAACACGCAGAAACCGGA+GGG | + | chr8.3:10389909-10389928 | None:intergenic | 45.0% | |
| TTTAGTCAATGGAGCCGCTA+TGG | - | chr8.3:10390432-10390451 | MS.gene58011:intron | 45.0% | |
| ! | AGCGGCTCCATTGACTAAAA+TGG | + | chr8.3:10390431-10390450 | None:intergenic | 45.0% |
| ! | ATTGTTGGTTGGGACTTCAG+TGG | - | chr8.3:10390271-10390290 | MS.gene58011:intron | 45.0% |
| ! | GATGGGAAAGTGTGGAATGT+AGG | + | chr8.3:10388706-10388725 | None:intergenic | 45.0% |
| ! | GTTTTTGTTGCTGGAGCTAC+TGG | - | chr8.3:10388928-10388947 | MS.gene58011:intron | 45.0% |
| ! | TAGTTTGTGCCACAGGCTTT+CGG | - | chr8.3:10389535-10389554 | MS.gene58011:intron | 45.0% |
| ! | TCAAGTTGCAGAAGTAGCTG+TGG | - | chr8.3:10390763-10390782 | MS.gene58011:intron | 45.0% |
| !! | CTTCAGTGGATTATTCTCGC+TGG | - | chr8.3:10390285-10390304 | MS.gene58011:intron | 45.0% |
| !! | GGGAAGAGATTTGGTTCTGA+AGG | + | chr8.3:10388758-10388777 | None:intergenic | 45.0% |
| !! | GTTTTGATATCAGCTGCCAC+TGG | - | chr8.3:10389682-10389701 | MS.gene58011:intron | 45.0% |
| !!! | GGGTTTTGCTGTTAAAGCTG+GGG | - | chr8.3:10388990-10389009 | MS.gene58011:CDS | 45.0% |
| !!! | TTAATTTTTTGTTTATATTA+TGG | - | chr8.3:10389593-10389612 | MS.gene58011:intron | 5.0% |
| AGATCATGGTATGCGCGCTT+AGG | + | chr8.3:10390851-10390870 | None:intergenic | 50.0% | |
| ATTGAGTAGCTGCCCCATAG+CGG | + | chr8.3:10390449-10390468 | None:intergenic | 50.0% | |
| CAGAAACCGGAGGGAAAATG+TGG | + | chr8.3:10389900-10389919 | None:intergenic | 50.0% | |
| CGCGCATACCATGATCTCTT+TGG | - | chr8.3:10390854-10390873 | MS.gene58011:CDS | 50.0% | |
| CTGGAGCTACTGGTAGTACT+GGG | - | chr8.3:10388938-10388957 | MS.gene58011:intron | 50.0% | |
| GTTTAACACGCAGAAACCGG+AGG | + | chr8.3:10389910-10389929 | None:intergenic | 50.0% | |
| TAGTCAATGGAGCCGCTATG+GGG | - | chr8.3:10390434-10390453 | MS.gene58011:intron | 50.0% | |
| TATGGGGCAGCTACTCAATC+CGG | - | chr8.3:10390450-10390469 | MS.gene58011:intron | 50.0% | |
| TCCCTTCCTCAACTTCGCAA+AGG | - | chr8.3:10388774-10388793 | MS.gene58011:CDS | 50.0% | |
| TGAATTGGCGAGTGTGGGAT+GGG | + | chr8.3:10388723-10388742 | None:intergenic | 50.0% | |
| ! | AGCTGCCACTGGTTAATGAG+TGG | - | chr8.3:10389693-10389712 | MS.gene58011:intron | 50.0% |
| ! | CGTTTTCGGACTCACCTTAG+TGG | - | chr8.3:10390489-10390508 | MS.gene58011:intron | 50.0% |
| ! | GAAGCAGTAGTTTGTGCCAC+AGG | - | chr8.3:10389528-10389547 | MS.gene58011:intron | 50.0% |
| !! | GGAATCGTTGGCTTATCCTG+AGG | - | chr8.3:10390784-10390803 | MS.gene58011:CDS | 50.0% |
| !! | GGATCGTTTTTCAACCCTCC+AGG | + | chr8.3:10390574-10390593 | None:intergenic | 50.0% |
| AGCGGTGAATTGGCGAGTGT+GGG | + | chr8.3:10388728-10388747 | None:intergenic | 55.0% | |
| AGGAAGAGGGAGCGGTGAAT+TGG | + | chr8.3:10388738-10388757 | None:intergenic | 55.0% | |
| GAGTGTGGGATGGGAAAGTG+TGG | + | chr8.3:10388714-10388733 | None:intergenic | 55.0% | |
| GCTGGAGCTACTGGTAGTAC+TGG | - | chr8.3:10388937-10388956 | MS.gene58011:intron | 55.0% | |
| GTGAATTGGCGAGTGTGGGA+TGG | + | chr8.3:10388724-10388743 | None:intergenic | 55.0% | |
| ! | GGATTTGCTTGCCCCATGGA+AGG | - | chr8.3:10389566-10389585 | MS.gene58011:intron | 55.0% |
| !! | GGTTCTGAAGGAAGAGGGAG+CGG | + | chr8.3:10388746-10388765 | None:intergenic | 55.0% |
| !! | TATGCGCGCTTAGGAGCATC+AGG | + | chr8.3:10390842-10390861 | None:intergenic | 55.0% |
| GAGCGGTGAATTGGCGAGTG+TGG | + | chr8.3:10388729-10388748 | None:intergenic | 60.0% | |
| ! | GCTGGGATTTGCTTGCCCCA+TGG | - | chr8.3:10389562-10389581 | MS.gene58011:intron | 60.0% |
| CACAGGCTTTCGGCCAGGCT+GGG | - | chr8.3:10389545-10389564 | MS.gene58011:intron | 65.0% | |
| GGGGCAAGCAAATCCCAGCC+TGG | + | chr8.3:10389561-10389580 | None:intergenic | 65.0% | |
| TGTGCCACAGGCTTTCGGCC+AGG | - | chr8.3:10389540-10389559 | MS.gene58011:intron | 65.0% | |
| CCACAGGCTTTCGGCCAGGC+TGG | - | chr8.3:10389544-10389563 | MS.gene58011:intron | 70.0% | |
| CCAGCCTGGCCGAAAGCCTG+TGG | + | chr8.3:10389547-10389566 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 10388676 | 10390895 | 10388676 | ID=MS.gene58011 |
| chr8.3 | mRNA | 10388676 | 10390895 | 10388676 | ID=MS.gene58011.t1;Parent=MS.gene58011 |
| chr8.3 | exon | 10390776 | 10390895 | 10390776 | ID=MS.gene58011.t1.exon1;Parent=MS.gene58011.t1 |
| chr8.3 | CDS | 10390776 | 10390895 | 10390776 | ID=cds.MS.gene58011.t1;Parent=MS.gene58011.t1 |
| chr8.3 | exon | 10390503 | 10390703 | 10390503 | ID=MS.gene58011.t1.exon2;Parent=MS.gene58011.t1 |
| chr8.3 | CDS | 10390503 | 10390703 | 10390503 | ID=cds.MS.gene58011.t1;Parent=MS.gene58011.t1 |
| chr8.3 | exon | 10389984 | 10390103 | 10389984 | ID=MS.gene58011.t1.exon3;Parent=MS.gene58011.t1 |
| chr8.3 | CDS | 10389984 | 10390103 | 10389984 | ID=cds.MS.gene58011.t1;Parent=MS.gene58011.t1 |
| chr8.3 | exon | 10388947 | 10389219 | 10388947 | ID=MS.gene58011.t1.exon4;Parent=MS.gene58011.t1 |
| chr8.3 | CDS | 10388947 | 10389219 | 10388947 | ID=cds.MS.gene58011.t1;Parent=MS.gene58011.t1 |
| chr8.3 | exon | 10388676 | 10388840 | 10388676 | ID=MS.gene58011.t1.exon5;Parent=MS.gene58011.t1 |
| chr8.3 | CDS | 10388676 | 10388840 | 10388676 | ID=cds.MS.gene58011.t1;Parent=MS.gene58011.t1 |
| Gene Sequence |
| Protein sequence |
