Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58468.t1 | KEH27042.1 | 93.7 | 79 | 4 | 1 | 1 | 79 | 60 | 137 | 3.70E-31 | 143.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58468.t1 | A0A072UBQ9 | 93.7 | 79 | 4 | 1 | 1 | 79 | 60 | 137 | 2.7e-31 | 143.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene58468 | 0.816611 | 5.06E-52 | -1.69E-46 |
| MS.gene049178 | MS.gene58468 | 0.826444 | 2.70E-54 | -1.69E-46 |
| MS.gene049378 | MS.gene58468 | 0.800599 | 1.34E-48 | -1.69E-46 |
| MS.gene049435 | MS.gene58468 | 0.802107 | 6.56E-49 | -1.69E-46 |
| MS.gene049501 | MS.gene58468 | 0.807028 | 6.17E-50 | -1.69E-46 |
| MS.gene049567 | MS.gene58468 | 0.806352 | 8.58E-50 | -1.69E-46 |
| MS.gene049647 | MS.gene58468 | 0.813876 | 2.05E-51 | -1.69E-46 |
| MS.gene049669 | MS.gene58468 | 0.801592 | 8.37E-49 | -1.69E-46 |
| MS.gene049958 | MS.gene58468 | 0.804298 | 2.31E-49 | -1.69E-46 |
| MS.gene050137 | MS.gene58468 | 0.809432 | 1.90E-50 | -1.69E-46 |
| MS.gene050156 | MS.gene58468 | 0.801898 | 7.24E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene58468 | 0.810769 | 9.77E-51 | -1.69E-46 |
| MS.gene050227 | MS.gene58468 | 0.827826 | 1.26E-54 | -1.69E-46 |
| MS.gene050228 | MS.gene58468 | 0.822682 | 2.08E-53 | -1.69E-46 |
| MS.gene050253 | MS.gene58468 | 0.802679 | 5.00E-49 | -1.69E-46 |
| MS.gene050355 | MS.gene58468 | 0.803013 | 4.27E-49 | -1.69E-46 |
| MS.gene050438 | MS.gene58468 | 0.815661 | 8.24E-52 | -1.69E-46 |
| MS.gene050442 | MS.gene58468 | 0.842757 | 2.13E-58 | -1.69E-46 |
| MS.gene050623 | MS.gene58468 | -0.800423 | 1.45E-48 | -1.69E-46 |
| MS.gene050719 | MS.gene58468 | 0.85538 | 6.56E-62 | -1.69E-46 |
| MS.gene050721 | MS.gene58468 | 0.853181 | 2.83E-61 | -1.69E-46 |
| MS.gene050724 | MS.gene58468 | 0.83107 | 2.06E-55 | -1.69E-46 |
| MS.gene050780 | MS.gene58468 | 0.88311 | 5.92E-71 | -1.69E-46 |
| MS.gene050815 | MS.gene58468 | 0.80866 | 2.78E-50 | -1.69E-46 |
| MS.gene050826 | MS.gene58468 | 0.804755 | 1.86E-49 | -1.69E-46 |
| MS.gene050844 | MS.gene58468 | 0.828154 | 1.05E-54 | -1.69E-46 |
| MS.gene050953 | MS.gene58468 | 0.800439 | 1.44E-48 | -1.69E-46 |
| MS.gene051207 | MS.gene58468 | 0.830844 | 2.34E-55 | -1.69E-46 |
| MS.gene051247 | MS.gene58468 | 0.844741 | 6.27E-59 | -1.69E-46 |
| MS.gene051265 | MS.gene58468 | 0.817309 | 3.53E-52 | -1.69E-46 |
| MS.gene051388 | MS.gene58468 | 0.805325 | 1.41E-49 | -1.69E-46 |
| MS.gene051490 | MS.gene58468 | 0.82667 | 2.39E-54 | -1.69E-46 |
| MS.gene051492 | MS.gene58468 | 0.809693 | 1.67E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene58468 | 0.812876 | 3.40E-51 | -1.69E-46 |
| MS.gene051744 | MS.gene58468 | 0.827153 | 1.83E-54 | -1.69E-46 |
| MS.gene051790 | MS.gene58468 | 0.803212 | 3.88E-49 | -1.69E-46 |
| MS.gene052012 | MS.gene58468 | 0.824432 | 8.10E-54 | -1.69E-46 |
| MS.gene052020 | MS.gene58468 | 0.82892 | 6.87E-55 | -1.69E-46 |
| MS.gene052384 | MS.gene58468 | 0.800495 | 1.40E-48 | -1.69E-46 |
| MS.gene052586 | MS.gene58468 | 0.810756 | 9.84E-51 | -1.69E-46 |
| MS.gene052773 | MS.gene58468 | 0.81496 | 1.18E-51 | -1.69E-46 |
| MS.gene052863 | MS.gene58468 | 0.806957 | 6.39E-50 | -1.69E-46 |
| MS.gene052896 | MS.gene58468 | 0.832309 | 1.02E-55 | -1.69E-46 |
| MS.gene053147 | MS.gene58468 | 0.819806 | 9.57E-53 | -1.69E-46 |
| MS.gene053226 | MS.gene58468 | 0.814448 | 1.53E-51 | -1.69E-46 |
| MS.gene053641 | MS.gene58468 | 0.845257 | 4.54E-59 | -1.69E-46 |
| MS.gene053647 | MS.gene58468 | 0.80381 | 2.92E-49 | -1.69E-46 |
| MS.gene053811 | MS.gene58468 | 0.82007 | 8.33E-53 | -1.69E-46 |
| MS.gene05414 | MS.gene58468 | 0.840626 | 7.76E-58 | -1.69E-46 |
| MS.gene05415 | MS.gene58468 | 0.807362 | 5.25E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene58468 | 0.835156 | 1.98E-56 | -1.69E-46 |
| MS.gene05427 | MS.gene58468 | 0.812097 | 5.03E-51 | -1.69E-46 |
| MS.gene054291 | MS.gene58468 | -0.807871 | 4.09E-50 | -1.69E-46 |
| MS.gene054467 | MS.gene58468 | 0.826406 | 2.76E-54 | -1.69E-46 |
| MS.gene054468 | MS.gene58468 | 0.85138 | 9.22E-61 | -1.69E-46 |
| MS.gene054472 | MS.gene58468 | 0.816528 | 5.28E-52 | -1.69E-46 |
| MS.gene054576 | MS.gene58468 | 0.829833 | 4.13E-55 | -1.69E-46 |
| MS.gene054581 | MS.gene58468 | 0.805818 | 1.11E-49 | -1.69E-46 |
| MS.gene05468 | MS.gene58468 | 0.913088 | 9.23E-84 | -1.69E-46 |
| MS.gene054775 | MS.gene58468 | 0.849766 | 2.62E-60 | -1.69E-46 |
| MS.gene055313 | MS.gene58468 | 0.818246 | 2.17E-52 | -1.69E-46 |
| MS.gene055356 | MS.gene58468 | 0.800999 | 1.11E-48 | -1.69E-46 |
| MS.gene055357 | MS.gene58468 | 0.827168 | 1.82E-54 | -1.69E-46 |
| MS.gene055358 | MS.gene58468 | 0.820222 | 7.69E-53 | -1.69E-46 |
| MS.gene055466 | MS.gene58468 | 0.804153 | 2.48E-49 | -1.69E-46 |
| MS.gene055608 | MS.gene58468 | 0.809805 | 1.58E-50 | -1.69E-46 |
| MS.gene055778 | MS.gene58468 | 0.825457 | 4.64E-54 | -1.69E-46 |
| MS.gene055818 | MS.gene58468 | 0.814851 | 1.25E-51 | -1.69E-46 |
| MS.gene055926 | MS.gene58468 | 0.845916 | 3.01E-59 | -1.69E-46 |
| MS.gene05604 | MS.gene58468 | 0.809594 | 1.75E-50 | -1.69E-46 |
| MS.gene056373 | MS.gene58468 | 0.806244 | 9.04E-50 | -1.69E-46 |
| MS.gene056552 | MS.gene58468 | 0.800548 | 1.37E-48 | -1.69E-46 |
| MS.gene057026 | MS.gene58468 | 0.837074 | 6.44E-57 | -1.69E-46 |
| MS.gene057693 | MS.gene58468 | 0.829777 | 4.26E-55 | -1.69E-46 |
| MS.gene057915 | MS.gene58468 | 0.832516 | 9.04E-56 | -1.69E-46 |
| MS.gene058084 | MS.gene58468 | 0.801365 | 9.32E-49 | -1.69E-46 |
| MS.gene058146 | MS.gene58468 | 0.814561 | 1.45E-51 | -1.69E-46 |
| MS.gene058168 | MS.gene58468 | 0.836109 | 1.13E-56 | -1.69E-46 |
| MS.gene058477 | MS.gene58468 | 0.807427 | 5.08E-50 | -1.69E-46 |
| MS.gene058636 | MS.gene58468 | 0.832973 | 6.96E-56 | -1.69E-46 |
| MS.gene058906 | MS.gene58468 | 0.802857 | 4.60E-49 | -1.69E-46 |
| MS.gene059084 | MS.gene58468 | 0.801633 | 8.21E-49 | -1.69E-46 |
| MS.gene059220 | MS.gene58468 | 0.800806 | 1.21E-48 | -1.69E-46 |
| MS.gene059249 | MS.gene58468 | 0.817192 | 3.75E-52 | -1.69E-46 |
| MS.gene059664 | MS.gene58468 | 0.80914 | 2.19E-50 | -1.69E-46 |
| MS.gene059725 | MS.gene58468 | 0.816504 | 5.35E-52 | -1.69E-46 |
| MS.gene060322 | MS.gene58468 | 0.833065 | 6.60E-56 | -1.69E-46 |
| MS.gene060572 | MS.gene58468 | 0.816807 | 4.57E-52 | -1.69E-46 |
| MS.gene060637 | MS.gene58468 | 0.804833 | 1.79E-49 | -1.69E-46 |
| MS.gene060781 | MS.gene58468 | 0.802011 | 6.87E-49 | -1.69E-46 |
| MS.gene060802 | MS.gene58468 | 0.81729 | 3.56E-52 | -1.69E-46 |
| MS.gene060803 | MS.gene58468 | 0.844016 | 9.81E-59 | -1.69E-46 |
| MS.gene060998 | MS.gene58468 | 0.830028 | 3.70E-55 | -1.69E-46 |
| MS.gene061040 | MS.gene58468 | -0.823108 | 1.65E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58468.t1 | MTR_6g084590 | 93.671 | 79 | 4 | 1 | 1 | 79 | 60 | 137 | 9.51e-46 | 143 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTAAAATATTTTGATGACAA+AGG | - | 42699:110701-110720 | MS.gene58468:CDS | 20.0% |
| ! | ATTAAGAAGAAGGAAAAGAT+TGG | - | 42699:110827-110846 | MS.gene58468:CDS | 25.0% |
| !!! | TATTTTGATGACAAAGGAAA+AGG | - | 42699:110707-110726 | MS.gene58468:CDS | 25.0% |
| ! | CCTTCTTCTTAATATTTTCC+CGG | + | 42699:110820-110839 | None:intergenic | 30.0% |
| !!! | AGCTTTTGATTTGAATGATG+TGG | - | 42699:110874-110893 | MS.gene58468:CDS | 30.0% |
| AAGAAGAAGGAAAAGATTGG+AGG | - | 42699:110830-110849 | MS.gene58468:CDS | 35.0% | |
| CCGGGAAAATATTAAGAAGA+AGG | - | 42699:110817-110836 | MS.gene58468:CDS | 35.0% | |
| ! | TTTGATGACAAAGGAAAAGG+TGG | - | 42699:110710-110729 | MS.gene58468:CDS | 35.0% |
| AAAAGGTGGAAGAGAATGGA+GGG | - | 42699:110724-110743 | MS.gene58468:CDS | 40.0% | |
| AAGGAAAAGGTGGAAGAGAA+TGG | - | 42699:110720-110739 | MS.gene58468:CDS | 40.0% | |
| TGATTTGAATGATGTGGCAC+CGG | - | 42699:110880-110899 | MS.gene58468:CDS | 40.0% | |
| GAAAAGGTGGAAGAGAATGG+AGG | - | 42699:110723-110742 | MS.gene58468:CDS | 45.0% | |
| GAAGGAAAAGATTGGAGGAG+AGG | - | 42699:110835-110854 | MS.gene58468:CDS | 45.0% | |
| GAGGAGAGGTGGTGATTATA+AGG | - | 42699:110849-110868 | MS.gene58468:CDS | 45.0% | |
| GAGGGAAACTCTTGAGAAAC+CGG | - | 42699:110742-110761 | MS.gene58468:CDS | 45.0% | |
| ! | TCTTAATATTTTCCCGGCCG+CGG | + | 42699:110814-110833 | None:intergenic | 45.0% |
| GGAAAAGATTGGAGGAGAGG+TGG | - | 42699:110838-110857 | MS.gene58468:CDS | 50.0% | |
| GGAAACTCTTGAGAAACCGG+CGG | - | 42699:110745-110764 | MS.gene58468:CDS | 50.0% | |
| CCACCACTTTCAGCAGCTTC+CGG | + | 42699:110902-110921 | None:intergenic | 55.0% | |
| CCGGAAGCTGCTGAAAGTGG+TGG | - | 42699:110899-110918 | MS.gene58468:CDS | 60.0% | |
| GATATCGTCGCTGACGACCG+CGG | - | 42699:110794-110813 | MS.gene58468:CDS | 60.0% | |
| GCACCGGAAGCTGCTGAAAG+TGG | - | 42699:110896-110915 | MS.gene58468:CDS | 60.0% | |
| TGAGAAACCGGCGGAGCTCG+CGG | - | 42699:110754-110773 | MS.gene58468:CDS | 65.0% | |
| CTGACGACCGCGAGCTCCGC+CGG | + | 42699:110764-110783 | None:intergenic | 75.0% | |
| TCGTCGCTGACGACCGCGGC+CGG | - | 42699:110798-110817 | MS.gene58468:CDS | 75.0% | |
| ! | CGTCGCTGACGACCGCGGCC+GGG | - | 42699:110799-110818 | MS.gene58468:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42699 | gene | 110689 | 110928 | 110689 | ID=MS.gene58468 |
| 42699 | mRNA | 110689 | 110928 | 110689 | ID=MS.gene58468.t1;Parent=MS.gene58468 |
| 42699 | exon | 110689 | 110928 | 110689 | ID=MS.gene58468.t1.exon1;Parent=MS.gene58468.t1 |
| 42699 | CDS | 110689 | 110928 | 110689 | ID=cds.MS.gene58468.t1;Parent=MS.gene58468.t1 |
| Gene Sequence |
| Protein sequence |
