AlfalfaGEDB | MS.gene76573

M. sativa cultivar XinJiangDaYe / MS.gene76573

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF07714.20 PF12819.10 PF13855.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76573.t1	XP_003597638.2	92	884	69	2	1	883	1	883	0.00E+00	1519.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76573.t1	O65924	49.1	851	407	11	8	844	10	848	1.5e-201	704.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76573.t1	G7IUL7	92.0	884	69	2	1	883	1	883	0.0e+00	1519.6

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049148	MS.gene76573	0.806839	6.77E-50	-1.69E-46
MS.gene049171	MS.gene76573	-0.810096	1.37E-50	-1.69E-46
MS.gene049217	MS.gene76573	-0.815911	7.25E-52	-1.69E-46
MS.gene049222	MS.gene76573	-0.82535	4.92E-54	-1.69E-46
MS.gene049232	MS.gene76573	-0.833495	5.16E-56	-1.69E-46
MS.gene049259	MS.gene76573	0.822847	1.90E-53	-1.69E-46
MS.gene049285	MS.gene76573	-0.812922	3.32E-51	-1.69E-46
MS.gene049320	MS.gene76573	-0.826009	3.43E-54	-1.69E-46
MS.gene049611	MS.gene76573	0.830842	2.34E-55	-1.69E-46
MS.gene049634	MS.gene76573	0.825965	3.52E-54	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene011081	MS.gene76573	PPI
MS.gene011087	MS.gene76573	PPI
MS.gene32297	MS.gene76573	PPI
MS.gene36602	MS.gene76573	PPI
MS.gene76573	MS.gene36715	PPI
MS.gene76573	MS.gene36619	PPI
MS.gene76573	MS.gene011099	PPI
MS.gene76573	MS.gene36729	PPI
MS.gene76573	MS.gene36388	PPI
MS.gene76573	MS.gene36389	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MS.gene76573.t1	MTR_2g100450	91.742	884	71	2	1	883	1	883	1665
MS.gene76573.t1	MTR_2g100450	91.555	746	61	2	1	745	1	745	1404
MS.gene76573.t1	MTR_2g100470	80.633	790	122	6	10	797	1	761	1296
MS.gene76573.t1	MTR_2g100450	91.150	678	59	1	206	883	2	678	1276
MS.gene76573.t1	MTR_8g015340	50.000	854	389	15	21	850	36	875	789
MS.gene76573.t1	MTR_8g014930	47.545	835	415	13	19	846	20	838	769
MS.gene76573.t1	MTR_8g015150	46.961	839	417	12	18	846	13	833	767
MS.gene76573.t1	MTR_8g014790	45.921	858	431	13	1	846	4	840	759
MS.gene76573.t1	MTR_8g015200	45.631	824	424	9	29	846	10	815	751
MS.gene76573.t1	MTR_8g014700	46.643	849	425	15	8	846	14	844	748

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MS.gene76573.t1	AT4G29990	50.519	867	402	18	4	857	5	857	830
MS.gene76573.t1	AT2G19210	48.399	843	408	12	18	846	21	850	789
MS.gene76573.t1	AT1G05700	45.444	856	443	10	4	849	5	846	753
MS.gene76573.t1	AT2G19230	46.956	854	425	13	5	846	6	843	753
MS.gene76573.t1	AT2G19230	47.073	854	423	13	5	846	6	842	750
MS.gene76573.t1	AT1G51800	44.582	886	449	14	1	859	1	871	746
MS.gene76573.t1	AT1G05700	44.444	882	445	12	4	849	5	877	744
MS.gene76573.t1	AT1G51860	45.505	901	426	20	9	871	10	883	729
MS.gene76573.t1	AT1G51880	46.395	860	416	18	10	848	7	842	728
MS.gene76573.t1	AT1G51880	46.744	860	413	20	10	848	25	860	728

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Find 184 sgRNAs with CRISPR-Local

Find 338 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGGTAATATATTGAGT+TGG	0.538228	2.3:-3228487	MS.gene76573:CDS
AAAAGTGATGTATTCAGTTT+TGG	0.224895	2.3:-3227829	MS.gene76573:CDS
AAATACTTGACCAGTCTTAT+AGG	0.240601	2.3:-3228859	MS.gene76573:CDS
AAATTGCAGATATCCTTGAG+CGG	0.682985	2.3:+3229032	None:intergenic
AACAATACCTGATAAATGGT+TGG	0.520110	2.3:+3228774	None:intergenic
AACATCCCTAGTGTAATAGA+TGG	0.436617	2.3:+3232421	None:intergenic
AACCATGAGGCATGTAGTTA+TGG	0.434194	2.3:-3227593	MS.gene76573:CDS
AAGGACTTACAAATATTCTA+TGG	0.304937	2.3:+3230774	None:intergenic
AAGTAAGAAACTGAAGTACA+AGG	0.624424	2.3:+3231034	None:intergenic
AATCCAGATCTTTGCTCCTC+AGG	0.404497	2.3:-3229638	MS.gene76573:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATTCTATCCAGATGTTC+AGG	+	chr2.3:3230194-3230213	None:intergenic	30.0%
AAAGTTGTGATGATCAATGA+AGG	-	chr2.3:3229942-3229961	MS.gene76573:intron	30.0%
AACAATACCTGATAAATGGT+TGG	+	chr2.3:3231431-3231450	None:intergenic	30.0%
AACTAAATAGTGCTTACCAT+CGG	+	chr2.3:3228978-3228997	None:intergenic	30.0%
AAGCTATCTTAGAGATCTTA+GGG	-	chr2.3:3228382-3228401	MS.gene76573:intron	30.0%
AAGTAAGAAACTGAAGTACA+AGG	+	chr2.3:3229171-3229190	None:intergenic	30.0%
AAGTACATGTGAAAACTGTT+TGG	+	chr2.3:3228461-3228480	None:intergenic	30.0%
AAGTTGTGATGATCAATGAA+GGG	-	chr2.3:3229943-3229962	MS.gene76573:intron	30.0%
ACAAACATGAACATAATCTG+AGG	+	chr2.3:3228102-3228121	None:intergenic	30.0%
ACACAAGTTCTTAACTATGA+AGG	+	chr2.3:3227686-3227705	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	3227462	3232762	3227462	ID=MS.gene76573
chr2.3	mRNA	3227462	3232762	3227462	ID=MS.gene76573.t1;Parent=MS.gene76573
chr2.3	exon	3232681	3232762	3232681	ID=MS.gene76573.t1.exon1;Parent=MS.gene76573.t1
chr2.3	CDS	3232681	3232762	3232681	ID=cds.MS.gene76573.t1;Parent=MS.gene76573.t1
chr2.3	exon	3231966	3232503	3231966	ID=MS.gene76573.t1.exon2;Parent=MS.gene76573.t1
chr2.3	CDS	3231966	3232503	3231966	ID=cds.MS.gene76573.t1;Parent=MS.gene76573.t1
chr2.3	exon	3231244	3231722	3231244	ID=MS.gene76573.t1.exon3;Parent=MS.gene76573.t1
chr2.3	CDS	3231244	3231722	3231244	ID=cds.MS.gene76573.t1;Parent=MS.gene76573.t1
chr2.3	exon	3230977	3231115	3230977	ID=MS.gene76573.t1.exon4;Parent=MS.gene76573.t1
chr2.3	CDS	3230977	3231115	3230977	ID=cds.MS.gene76573.t1;Parent=MS.gene76573.t1
chr2.3	exon	3230784	3230855	3230784	ID=MS.gene76573.t1.exon5;Parent=MS.gene76573.t1
chr2.3	CDS	3230784	3230855	3230784	ID=cds.MS.gene76573.t1;Parent=MS.gene76573.t1
chr2.3	exon	3230608	3230679	3230608	ID=MS.gene76573.t1.exon6;Parent=MS.gene76573.t1
chr2.3	CDS	3230608	3230679	3230608	ID=cds.MS.gene76573.t1;Parent=MS.gene76573.t1
chr2.3	exon	3229855	3229935	3229855	ID=MS.gene76573.t1.exon7;Parent=MS.gene76573.t1
chr2.3	CDS	3229855	3229935	3229855	ID=cds.MS.gene76573.t1;Parent=MS.gene76573.t1
chr2.3	exon	3229516	3229670	3229516	ID=MS.gene76573.t1.exon8;Parent=MS.gene76573.t1
chr2.3	CDS	3229516	3229670	3229516	ID=cds.MS.gene76573.t1;Parent=MS.gene76573.t1
chr2.3	exon	3229023	3229258	3229023	ID=MS.gene76573.t1.exon9;Parent=MS.gene76573.t1
chr2.3	CDS	3229023	3229258	3229023	ID=cds.MS.gene76573.t1;Parent=MS.gene76573.t1
chr2.3	exon	3228782	3228908	3228782	ID=MS.gene76573.t1.exon10;Parent=MS.gene76573.t1
chr2.3	CDS	3228782	3228908	3228782	ID=cds.MS.gene76573.t1;Parent=MS.gene76573.t1
chr2.3	exon	3228450	3228512	3228450	ID=MS.gene76573.t1.exon11;Parent=MS.gene76573.t1
chr2.3	CDS	3228450	3228512	3228450	ID=cds.MS.gene76573.t1;Parent=MS.gene76573.t1
chr2.3	exon	3228145	3228334	3228145	ID=MS.gene76573.t1.exon12;Parent=MS.gene76573.t1
chr2.3	CDS	3228145	3228334	3228145	ID=cds.MS.gene76573.t1;Parent=MS.gene76573.t1
chr2.3	exon	3227462	3227879	3227462	ID=MS.gene76573.t1.exon13;Parent=MS.gene76573.t1
chr2.3	CDS	3227462	3227879	3227462	ID=cds.MS.gene76573.t1;Parent=MS.gene76573.t1

Gene Sequence

>MS.gene76573

ATGGCAAGACGCTTGCTATCTATAGTGTTTCTACTTTCTTTGACTCTTCCTTTCATTGTTCATGCTCAGAACAACCAATCAGGTTTGGTAGTCTAATCTCATAACATATTATTCTGCATATAGACTACATAGGACTGCTTCATGTCCTTAGAACATAAGTAGCGTTGAGCCGCCTACAAGTCAATGTTGTGTTCGGTGTCTGTGTCTGTATTTGATTCGGTCCTTCATAGTTAAGAACTTGTGTTATCCTCTTTTGCAGGCTTCATTAGTATTGACTGTGGACTAGTGGATGAACCACGCTACACAGATGAAACCACTTCCATCTATTACACTAGGGATGTTAATTTCACAGATACTGGTGTAAGCCACAGCATATCATCTAAACTCAAGGCATCCCTTGAAAGACAGTTTTGGAATGTACGAAGCTTCCCAGAAGGAACAAGGAACTGCTACACGCTTTATGTTTCACATGGAAGTAGCAAGAAATATTTAGTCCGGGCCAGTTTTGTGTATGGTAATTATGATGGAAAAGATTCTCTTCCTGAATTTGATATCTATCTTGGAGTCAAATGGTGGGAATCAGTGGTGTTTGAGAATTCATCTAGTGTAATAACCAAGGAAATCATTTATGCTGCATCCTCAGATTATGTTCATGTTTGTTTGTTTAATACTGGCAAGGGTACACCTTTTATTTCAGTTTTAGAGCTTAGAGTTCTTAAAAGTGATGCTTATCTTGATAACTCTTTGGAGCTTTTGGGGAGGTTTGATATAGGCTCAAAGGATGATAAAAAAATCAGGTGAGCCATCTCACTTATAAGCAATAAACGCTTATCATACAAGTGCTTATGTATAATCTATTTCTATAACAAAAGGTATAATAAAGTCAAATTATTTTCATATCAGTTATATGTTGTTTTTATAAGCTATCTTAGAGATCTTAGGGTAAAAGTTTAAAACAGCTTGTGGACACGTCATAAACTGTTTCTATAAGCTCTCCCAAACAGTTTTCACATGTACTTACACTTTTTCCTTTTCCAAAGATATCCAGATGATATTTATGACAGAACATGGACACCTTATAACTCAATTGAATGGAAAAAAATAGACACTTCACTCACCATAGACCAAAGAGCACCTCCATTCGACTTTTTTACGGAACCACCCTCCACTGTCATGAGAACAACAGCAATTCCAGCAAATTCCAGTGACAACATGGAATTTTTCTTCCTTCCCAAGTACAATTCCTCCAGATATTATGTGTACGTGTACTTTGCTGAAATCCAGAAGCTCCAGGCAAATCAAATCAGAGAGTTCAACATTTTTGTAAATGGGAATCTTCTAAGTAGTGAAGTCAATCCTTTGTATCTTAAAAACGGGTATTATGTATTAGCTAGAGGTGAAACTAAGTTGGAAATTTGGCTTAATAAGACTAGTAGATCAACTCTTCCACCGCTATTCAATGCCGTCGAGATATATATGGTAAAGGACTTCCTACAATCAGAAACATATCAAACCGATGGTAAGCACTATTTAGTTAGATACTGATTTTACTCTCAAATTTCTATTTACAGTTAATTCAAGACCATGCGCTTCATTATTCACATGCATTATTCTTACAATCTTATCATGTTGATGCTATCCTAACAGTTGATGCCATCCTAAATGTCAAGTCAATTTATGGGATCAAGAGAAACTGGCAAGGAGATCCTTGTACTTCAGTTTCTTACTTGTGGAATGGTTTAAACTGCAGCTATGCTGGTACTGATTCACCAAGGATCATATACCTGTAAGTTATGTGGCATGAAATGAAATACATATGTTGATCATCCTTTAGTTTAGTAGTTTTCACTTTTAACTTTTTAGTATAGTCTTCAATGATATTAATGAATTTCATAACCATGCTTTAGGAACTTAACTTCCAGCGGTTTAATTGGAACTATAGCTGCCGGCATATCCAATCTGAAGTCCATAGAATATTTGTAAGTCCTTTAATCTATTACACACGAATAGTAAGCAATATAGTTGGTCACAAAAATGAAGCCTGCTCCTAAAGTTGAGTAAAAACATTTTGTTCCAATTTTAGGGATTTGTCAAATAACAATTTGACTGGAGCTGTGCCTGATTTCCTATCTCAGCTGCGTTTCTTGAGAGTTCTGTAAGTCAACTATCTTCATATGGTTAAATTCTATACAGTGGCAAAGCTTTCAAAGTGCTAGGTTCTCCAAGGTGGACTTGATTTTTCAATTAATTTTATTGCTTGAGCTGTTAAAAAAAAAATATTATGGTAAGAATGCCAATGTGCACCTTATCGTATGAACCTGAACCTCAGTAGCACATCACGGGAAGCTCTGAGATTAGGCGTGTGCGACATGCATCGGTGTCTGACATCTCAGACAAGTGCCCTGAAACAAAACTATTTTTCTTTGATTTGACACACCTAAGACACTCTTTGAACACTTCTGAACACTTCGACCAGGGTCAAAGTTGTGATGATCAATGAAGGGGTTAAAGATGATTACATCATTTTTAGCTTCTCAGTATGGTAGATGGATGAATTAAGATGAAAAACTATGTTTTTTTTTTTTTTTTTAATAAAACAACTTGCTCAATTTTTAGCTTCTCTGTGTGTGTGTGTGTGTTGGGTGTTGGTGTCCTATATTTTTTAAATTAGCAGTGTTAACATGTCTGTGTCGTTTCTAGTGTCCGTGTTAGAGTTCGCGCTTCATAGACCTGAACATCTGGATAGAATTTTGACGTGCTATATGTTTTTTTTACATATTTTTATGCAAGCATGTATAAAGGGTAATCTTGTATTTGTTTATGGAAGAAATCTTCAAGGAAATCAGTTATCAGGAGCAATTCCATTACAACTCCTTGTGCGTTCAGAGAATAGTACGCTCCAATTCAAGTATGTTGGATTTCAAGTTTCACATTTTTTTTTGAAGTTAAAGTTTCACATTTAAGTAATATTTATTCTGTTTTCATTTTCCATGTAATGAGCTAGTGATTTATATCATAATTATATTACAAAAATGTAAGGTATCAGGCAATATCAAATAAATAACACGATTAATTGTTACTGTAAATGCAAGTTTTGGTGGAAATCCAGATCTTTGCTCCTCAGGTTCATGCAACAAATGGAATGGGAATAAGGTTGTAGTTCCATTGGTAACATCAATTGGTGGAGCTTTCTTGATACTAGCAGTAGCAGTGATTTCTTTTCGCATTTACAATAAGAGAAACCGAGGTACATTTTGTTTTCTATTCTATTGACCTTGTTGGGAGCCAAACAACATGTATAAAAATATAGGATTTAATTGGTATGCAATGTAAATTTAAAATCTTTTACACTGTCAAACTAATTATATACCATCTTAAATGTGACAATTTCATATGAATGTGAAGATTAATGATTGGTTGACTGACACACTCTGTGTGTCAAAAGTAGACTCAAACACTTACAATACTAATTCAAGAATAAAAACTGTGTTTGCTTCTTCCCAGTTTCCCATGAGGTGATCAAGCTGGGTGCTAATTCAAGAATAAAGCAGGAACTAGAGTCAAAGAAACAAGAATTCACCTATGAAGAAGTATTAAGAACTACCAGAAACTTTGAAAAGATTCTAGGGAAAGGAGCATCTGGAACAGTATACCATGGCTGGATTGATCAAAATACTGAGGTAGCTGTCAAAATGCTATCTTCCTCATCCGCTCAAGGATATCTGCAATTTCAAGCAGAGGTTGTATCATGAATTCACTTGCAAATTGTATCATGCATATGCGTATGTATACGGTATATATATAGTTAATGAATTTTATGTTAATTAAATGTAGTTTTCCTTTGTGATTCATAGGCCAAATTTTTTGCTACAGTTCATCACAAATACTTGACCAGTCTTATAGGTTACTGTGATGATGGCACCAATATGGCTCTTATCTATGAGTATATGGCTAATGGAGACTTATCCAACCATTTATCAGGTATTGTTTATGATCATGTCAGTCCATTAATTTTATTCTCTCTAAAAGGTTATAATTCTAGTTACAGTTATGTTATATTTATTCAAGTAACTTCTAAATAGTCAAGGGATGTCCAAAAAACACAAACCTGGTAAATTAAGTCAAAGATTTTTTCTACTTGTAAAAATAGGAAAACAATACTTCTTTGTTATTCTAAACCAAATGCCAAGATAGATATGTCTGTTTTCCTGGTTATGAATTTCTTGGTTAAGATTGTTTTATGAATGCAGATAAAAACGGTAATATATTGAGTTGGAATCAAAGACTTCAAATTGCAGTGGATACTGCAGAAGGTGAGTATGAATCCTTTTTCCCATTAGTTTCATGATTTTCATCAATCAAAACATTTCTCATAATGAGAAATACTTTAGGCAACTAACTTAACTTAATTTGAACTTTCAAATCTAGGATTGGAGTATCTGCATCATGGTTGCAATCCACCAATTGTTCATAGAGATGTTAAGTCTAAAAACATCTTACTAAATGAAAAATTACAAGGTAAATTAGCTGATTTTGGATTGTCCAAGATCTTTCCTAATGAAGGTGAAACTCATCTCTCCACAGCTATTGCTGGCACGCCTGGATACCTCGACCCGGAGTAAGATATCCTTAATATAAGTTCCATTTGGAAAAAAAAAGTTACACAAAATGGATAAATTAATACAATATTTTATTTTCTTAATACATGTGAAATTGTTAAAATGTAACTTCTAAGAATGGATAGAAGTAGTACTATTTTAACTCAAACCCAAATGGAAAGAGCCAAAGGCCACTCAAGGGTGTTACCTTTGAGAAAACATTTTTGTTTTTCCTTTTCACTTTTGGTTTATATATAGATTAAGTTGCAAATTTGTATTTTTCAGGTACAATAGATTAAGCAGGTTGAGAGAAAAAAGTGATGTATTCAGTTTTGGGGTGGTTTTGCTGGAGATAATTACAGGACAACCTGCCATAACCAAAACTGAAAACAAAATCCATATTGTTCAATTGGTTGGTGACATGCTGCTGGAAAGGGAGGTAAAAGATATTGTTGATCCCAGATTGCAGGGAGACTTTGATATTAACTATGCAACTAAAGCTTTGGATACCGCAATGGCTTGTGTAGCACAATCTTCCATCAATAGACCAACCATGAGGCATGTAGTTATGGAACTGAAACAATGTTTGGAAAACAAGATCACTTATCCGTCTGACTCCAAATACACTCTTGAGAGTTTACCTAGTGCATTATATTCTGTCTCCTTTGATAGAATCAGTGGCGAGAGCTCTCTAGCGAGGTAG

Protein sequence

>MS.gene76573.t1

MARRLLSIVFLLSLTLPFIVHAQNNQSGFISIDCGLVDEPRYTDETTSIYYTRDVNFTDTGVSHSISSKLKASLERQFWNVRSFPEGTRNCYTLYVSHGSSKKYLVRASFVYGNYDGKDSLPEFDIYLGVKWWESVVFENSSSVITKEIIYAASSDYVHVCLFNTGKGTPFISVLELRVLKSDAYLDNSLELLGRFDIGSKDDKKIRYPDDIYDRTWTPYNSIEWKKIDTSLTIDQRAPPFDFFTEPPSTVMRTTAIPANSSDNMEFFFLPKYNSSRYYVYVYFAEIQKLQANQIREFNIFVNGNLLSSEVNPLYLKNGYYVLARGETKLEIWLNKTSRSTLPPLFNAVEIYMVKDFLQSETYQTDVDAILNVKSIYGIKRNWQGDPCTSVSYLWNGLNCSYAGTDSPRIIYLNLTSSGLIGTIAAGISNLKSIEYLDLSNNNLTGAVPDFLSQLRFLRVLNLQGNQLSGAIPLQLLVRSENSTLQFNFGGNPDLCSSGSCNKWNGNKVVVPLVTSIGGAFLILAVAVISFRIYNKRNRVSHEVIKLGANSRIKQELESKKQEFTYEEVLRTTRNFEKILGKGASGTVYHGWIDQNTEVAVKMLSSSSAQGYLQFQAEAKFFATVHHKYLTSLIGYCDDGTNMALIYEYMANGDLSNHLSDKNGNILSWNQRLQIAVDTAEGLEYLHHGCNPPIVHRDVKSKNILLNEKLQGKLADFGLSKIFPNEGETHLSTAIAGTPGYLDPEYNRLSRLREKSDVFSFGVVLLEIITGQPAITKTENKIHIVQLVGDMLLEREVKDIVDPRLQGDFDINYATKALDTAMACVAQSSINRPTMRHVVMELKQCLENKITYPSDSKYTLESLPSALYSVSFDRISGESSLAR