AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82036


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82036.t1 MTR_3g070960 95.238 231 11 0 1 231 1 231 2.34e-162 448
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82036.t1 AT3G56010 58.519 135 44 4 99 231 77 201 3.42e-48 157

Find 52 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTCATTAGTCATTAAAA+TGG 0.237690 3.2:-64543310 None:intergenic
AACAAATTACCAGGGGCTTC+TGG 0.243956 3.2:-64543140 None:intergenic
TTCAACAGATGCTAGTAATA+AGG 0.322512 3.2:+64542833 MS.gene82036:CDS
TGAGATGATCGTGTGGTTAC+AGG 0.325931 3.2:-64542975 None:intergenic
TTTGTCAAGGAGAAAAGATT+TGG 0.326672 3.2:+64542777 MS.gene82036:CDS
TTTATCAGCACAGTGATGTT+TGG 0.378545 3.2:+64543254 MS.gene82036:CDS
GTGTGCTCTGAGTGGCCTTC+GGG 0.390366 3.2:-64542725 None:intergenic
AGAAAGTGAGAGAGAGGATA+AGG 0.396564 3.2:+64542997 MS.gene82036:CDS
TCAAAAGTCCAACCTTCCTT+TGG 0.411926 3.2:-64543185 None:intergenic
AGTGTGCTCTGAGTGGCCTT+CGG 0.416107 3.2:-64542726 None:intergenic
GTCTGTGATTCTGCCTGAGC+AGG 0.422298 3.2:-64542909 None:intergenic
GTGGTTACAGGTTGCGACTT+AGG 0.426739 3.2:-64542963 None:intergenic
GGTAGATGTTAACTGATCAT+TGG 0.433318 3.2:-64542883 None:intergenic
CAAACTATCACGATGCATCT+AGG 0.448156 3.2:+64542682 MS.gene82036:CDS
CTGTCATCAAACAAATTACC+AGG 0.451100 3.2:-64543149 None:intergenic
GATGCTAGTAATAAGGATAC+TGG 0.451608 3.2:+64542840 MS.gene82036:CDS
GGCCTTCGGGAAAGAAGCTC+CGG 0.457076 3.2:-64542712 None:intergenic
GAAAGTGAGAGAGAGGATAA+GGG 0.460702 3.2:+64542998 MS.gene82036:CDS
TGATTCTGCCTGAGCAGGAT+TGG 0.469008 3.2:-64542904 None:intergenic
AAAACAGCTAAATCAGAAAT+GGG 0.480346 3.2:+64543053 MS.gene82036:CDS
GCAGATCCATTGGAAACCTC+TGG 0.503333 3.2:-64542933 None:intergenic
CGTTGAGGTGAAGCTGATGA+TGG 0.510936 3.2:-64541780 None:intergenic
AACATCTACCAATCCTGCTC+AGG 0.512154 3.2:+64542896 MS.gene82036:CDS
AGGAGATGCTGCAGATCCAT+TGG 0.516503 3.2:-64542943 None:intergenic
CTAGTAATAAGGATACTGGC+AGG 0.528322 3.2:+64542844 MS.gene82036:CDS
TGCCGGAGCTTCTTTCCCGA+AGG 0.532920 3.2:+64542710 MS.gene82036:CDS
ACTTACATTGTGTGGAAAGT+TGG 0.543190 3.2:+64543281 MS.gene82036:CDS
TTGGAACAACTTACATTGTG+TGG 0.560213 3.2:+64543273 MS.gene82036:CDS
GAGGGATGCCAAAGGAAGGT+TGG 0.568563 3.2:+64543177 MS.gene82036:CDS
CATCTCAGAAAGTGAGAGAG+AGG 0.580497 3.2:+64542991 MS.gene82036:CDS
ACACTCGTTTCGATAACGTC+GGG 0.594355 3.2:+64542744 MS.gene82036:CDS
TTGTTTGATGACAGCAAACG+AGG 0.599920 3.2:+64543158 MS.gene82036:CDS
TCTGGACTTCCAGAAGCCCC+TGG 0.604678 3.2:+64543131 MS.gene82036:CDS
GATGCATCTAGGTATTCTGC+CGG 0.605736 3.2:+64542693 MS.gene82036:CDS
CACTTTCTGAGATGATCGTG+TGG 0.606839 3.2:-64542982 None:intergenic
AAACGAGGGATGCCAAAGGA+AGG 0.625366 3.2:+64543173 MS.gene82036:CDS
TCGAGTTCTTAACCAGTCCA+AGG 0.632326 3.2:+64543025 MS.gene82036:CDS
CATCATCAGCTTCACCTCAA+CGG 0.635332 3.2:+64541781 MS.gene82036:CDS
TAGTAATAAGGATACTGGCA+GGG 0.635455 3.2:+64542845 MS.gene82036:CDS
GGGAACAAGAATGTTTGTCA+AGG 0.643523 3.2:+64542764 MS.gene82036:CDS
CAGCAAACGAGGGATGCCAA+AGG 0.649716 3.2:+64543169 MS.gene82036:CDS
CAGACACCAGAGGTTTCCAA+TGG 0.649775 3.2:+64542927 MS.gene82036:CDS
GTTGAGGTGAAGCTGATGAT+GGG 0.654569 3.2:-64541779 None:intergenic
AGAAAGAGATCAGAACCACC+AGG 0.655199 3.2:-64543212 None:intergenic
TGTTTGATGACAGCAAACGA+GGG 0.666281 3.2:+64543159 MS.gene82036:CDS
AGAAATGGGAAGTAGTGTGT+TGG 0.668878 3.2:+64543067 MS.gene82036:CDS
CACACTCGTTTCGATAACGT+CGG 0.679278 3.2:+64542743 MS.gene82036:CDS
GGCAGAATCACAGACACCAG+AGG 0.679651 3.2:+64542917 MS.gene82036:CDS
TGTCATCAAACAAATTACCA+GGG 0.683437 3.2:-64543148 None:intergenic
AGTGAGAGAGAGGATAAGGG+CGG 0.687543 3.2:+64543001 MS.gene82036:CDS
GTCATCAAACAAATTACCAG+GGG 0.699685 3.2:-64543147 None:intergenic
CGAAACGAGTGTGCTCTGAG+TGG 0.707929 3.2:-64542733 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATAATTGAATTATATTGA+AGG - chr3.2:64542597-64542616 None:intergenic 15.0%
!! ATTATCTTCTAATATGTAAT+AGG + chr3.2:64542537-64542556 MS.gene82036:intron 15.0%
!! AAGGAAATATGACTATTTAA+AGG - chr3.2:64542257-64542276 None:intergenic 20.0%
!! AGGAAATATGACTATTTAAA+GGG - chr3.2:64542256-64542275 None:intergenic 20.0%
!! ATACTAAACATTATTTGACT+TGG + chr3.2:64541936-64541955 MS.gene82036:intron 20.0%
!!! AGTTTATAGATTTAAGGTTA+TGG + chr3.2:64541851-64541870 MS.gene82036:intron 20.0%
!!! CACTTTAGTTTATAGATTTA+AGG + chr3.2:64541845-64541864 MS.gene82036:intron 20.0%
!!! TACTAAACATTATTTGACTT+GGG + chr3.2:64541937-64541956 MS.gene82036:intron 20.0%
! AAAAACAGCTAAATCAGAAA+TGG + chr3.2:64543052-64543071 MS.gene82036:CDS 25.0%
! AAAACAGCTAAATCAGAAAT+GGG + chr3.2:64543053-64543072 MS.gene82036:CDS 25.0%
! CCCAAAATTGACATATAAAA+GGG - chr3.2:64542496-64542515 None:intergenic 25.0%
! TAAATAGTCATATTTCCTTG+TGG + chr3.2:64542258-64542277 MS.gene82036:intron 25.0%
! TAACAACGTGTTATGTTAAT+CGG + chr3.2:64542008-64542027 MS.gene82036:intron 25.0%
! TATAAAATGCGCTATATCTA+TGG + chr3.2:64542095-64542114 MS.gene82036:intron 25.0%
! TCCCAAAATTGACATATAAA+AGG - chr3.2:64542497-64542516 None:intergenic 25.0%
! TTAACATAAAGCAATTTCAC+TGG - chr3.2:64542075-64542094 None:intergenic 25.0%
!! ATTTGCTTGTAATTGATTCT+TGG + chr3.2:64541907-64541926 MS.gene82036:intron 25.0%
!! CCCTTTTATATGTCAATTTT+GGG + chr3.2:64542493-64542512 MS.gene82036:intron 25.0%
!! TCCCTTTTATATGTCAATTT+TGG + chr3.2:64542492-64542511 MS.gene82036:intron 25.0%
!!! ATTTCTGATTTAGCTGTTTT+TGG - chr3.2:64543053-64543072 None:intergenic 25.0%
!!! TTGTTTGATGATGAAAATGT+TGG + chr3.2:64542445-64542464 MS.gene82036:intron 25.0%
ATCTCAATTGATCCAATTGA+AGG - chr3.2:64542183-64542202 None:intergenic 30.0%
CAATTGGATCAATTGAGATA+TGG + chr3.2:64542184-64542203 MS.gene82036:intron 30.0%
CACTATTTGCAACTGAAAAA+CGG - chr3.2:64542806-64542825 None:intergenic 30.0%
TGTCATCAAACAAATTACCA+GGG - chr3.2:64543151-64543170 None:intergenic 30.0%
TTCAACAGATGCTAGTAATA+AGG + chr3.2:64542833-64542852 MS.gene82036:CDS 30.0%
TTTCATTATCCCTTAGAAGT+TGG + chr3.2:64542656-64542675 MS.gene82036:intron 30.0%
TTTGTCAAGGAGAAAAGATT+TGG + chr3.2:64542777-64542796 MS.gene82036:CDS 30.0%
! AGTTTGTTTCCAACTTCTAA+GGG - chr3.2:64542668-64542687 None:intergenic 30.0%
! TAAGAAGAGTGTGTTAGTTA+TGG + chr3.2:64541874-64541893 MS.gene82036:intron 30.0%
!! CTTTTAAAGAAAGTGACAGA+GGG + chr3.2:64543093-64543112 MS.gene82036:CDS 30.0%
!! GGTGAGATTTATTGATTCAA+TGG + chr3.2:64542205-64542224 MS.gene82036:intron 30.0%
!! TAGTTTGTTTCCAACTTCTA+AGG - chr3.2:64542669-64542688 None:intergenic 30.0%
ACTTACATTGTGTGGAAAGT+TGG + chr3.2:64543281-64543300 MS.gene82036:CDS 35.0%
CTGTCATCAAACAAATTACC+AGG - chr3.2:64543152-64543171 None:intergenic 35.0%
CTTTGCAGTTATGTTGTGAT+CGG + chr3.2:64542394-64542413 MS.gene82036:intron 35.0%
GAACTTAACAAAGATCCACA+AGG - chr3.2:64542276-64542295 None:intergenic 35.0%
GATGCTAGTAATAAGGATAC+TGG + chr3.2:64542840-64542859 MS.gene82036:CDS 35.0%
GGTAGATGTTAACTGATCAT+TGG - chr3.2:64542886-64542905 None:intergenic 35.0%
GTCATCAAACAAATTACCAG+GGG - chr3.2:64543150-64543169 None:intergenic 35.0%
TAATCGGAAACAGTATTCCT+GGG + chr3.2:64542024-64542043 MS.gene82036:intron 35.0%
TAGTAATAAGGATACTGGCA+GGG + chr3.2:64542845-64542864 MS.gene82036:CDS 35.0%
TGAAGGACTTACTGATCAAA+TGG - chr3.2:64542166-64542185 None:intergenic 35.0%
TGATCAGTAAGTCCTTCAAT+TGG + chr3.2:64542168-64542187 MS.gene82036:intron 35.0%
TTAATCGGAAACAGTATTCC+TGG + chr3.2:64542023-64542042 MS.gene82036:intron 35.0%
TTGGAACAACTTACATTGTG+TGG + chr3.2:64543273-64543292 MS.gene82036:CDS 35.0%
TTTATCAGCACAGTGATGTT+TGG + chr3.2:64543254-64543273 MS.gene82036:CDS 35.0%
!! GCTTTTAAAGAAAGTGACAG+AGG + chr3.2:64543092-64543111 MS.gene82036:CDS 35.0%
!!! TTTAGCTGTTTTTGGTTCCT+TGG - chr3.2:64543045-64543064 None:intergenic 35.0%
AAAGTGACAGAGGGAAAAAG+AGG + chr3.2:64543102-64543121 MS.gene82036:CDS 40.0%
AGAAAGTGAGAGAGAGGATA+AGG + chr3.2:64542997-64543016 MS.gene82036:CDS 40.0%
AGAAATGGGAAGTAGTGTGT+TGG + chr3.2:64543067-64543086 MS.gene82036:CDS 40.0%
CAAACTATCACGATGCATCT+AGG + chr3.2:64542682-64542701 MS.gene82036:CDS 40.0%
CATTAAACCAAAGTTCCAGG+TGG + chr3.2:64542312-64542331 MS.gene82036:intron 40.0%
CTAGTAATAAGGATACTGGC+AGG + chr3.2:64542844-64542863 MS.gene82036:CDS 40.0%
CTATCATAAGAAGTTCACCC+AGG - chr3.2:64542044-64542063 None:intergenic 40.0%
CTATGGTGCACATGTTAAGT+AGG + chr3.2:64542112-64542131 MS.gene82036:intron 40.0%
GAAAGTGAGAGAGAGGATAA+GGG + chr3.2:64542998-64543017 MS.gene82036:CDS 40.0%
GATAGAAAAACGTACCGTTG+AGG - chr3.2:64541798-64541817 None:intergenic 40.0%
GGGAACAAGAATGTTTGTCA+AGG + chr3.2:64542764-64542783 MS.gene82036:CDS 40.0%
GTGCATTAAACCAAAGTTCC+AGG + chr3.2:64542309-64542328 MS.gene82036:intron 40.0%
TCAAAAGTCCAACCTTCCTT+TGG - chr3.2:64543188-64543207 None:intergenic 40.0%
! GTTAAGTAGGTGCTGTACTA+TGG + chr3.2:64542125-64542144 MS.gene82036:intron 40.0%
! TGTTTGATGACAGCAAACGA+GGG + chr3.2:64543159-64543178 MS.gene82036:CDS 40.0%
! TTGTTTGATGACAGCAAACG+AGG + chr3.2:64543158-64543177 MS.gene82036:CDS 40.0%
!!! GGTTGGACTTTTGATTTTCC+TGG + chr3.2:64543194-64543213 MS.gene82036:CDS 40.0%
!!! TGGACTTTTGATTTTCCTGG+TGG + chr3.2:64543197-64543216 MS.gene82036:CDS 40.0%
AACAAATTACCAGGGGCTTC+TGG - chr3.2:64543143-64543162 None:intergenic 45.0%
AACATCTACCAATCCTGCTC+AGG + chr3.2:64542896-64542915 MS.gene82036:CDS 45.0%
ACACTCGTTTCGATAACGTC+GGG + chr3.2:64542744-64542763 MS.gene82036:CDS 45.0%
AGAAAGAGATCAGAACCACC+AGG - chr3.2:64543215-64543234 None:intergenic 45.0%
AGAAAGTCCACCTGGAACTT+TGG - chr3.2:64542322-64542341 None:intergenic 45.0%
CACACTCGTTTCGATAACGT+CGG + chr3.2:64542743-64542762 MS.gene82036:CDS 45.0%
CATCATCAGCTTCACCTCAA+CGG + chr3.2:64541781-64541800 MS.gene82036:CDS 45.0%
CATCTCAGAAAGTGAGAGAG+AGG + chr3.2:64542991-64543010 MS.gene82036:CDS 45.0%
GATGCATCTAGGTATTCTGC+CGG + chr3.2:64542693-64542712 MS.gene82036:CDS 45.0%
GGGAAAAAGAGGAGAAGATC+TGG + chr3.2:64543113-64543132 MS.gene82036:CDS 45.0%
GTTGAGGTGAAGCTGATGAT+GGG - chr3.2:64541782-64541801 None:intergenic 45.0%
TCGAGTTCTTAACCAGTCCA+AGG + chr3.2:64543025-64543044 MS.gene82036:CDS 45.0%
TGAGATGATCGTGTGGTTAC+AGG - chr3.2:64542978-64542997 None:intergenic 45.0%
! CATTCTAGAGAAAGTCCACC+TGG - chr3.2:64542330-64542349 None:intergenic 45.0%
!! CACTTTCTGAGATGATCGTG+TGG - chr3.2:64542985-64543004 None:intergenic 45.0%
!!! CTGTTTTTGGTTCCTTGGAC+TGG - chr3.2:64543040-64543059 None:intergenic 45.0%
AAACGAGGGATGCCAAAGGA+AGG + chr3.2:64543173-64543192 MS.gene82036:CDS 50.0%
AGGAGATGCTGCAGATCCAT+TGG - chr3.2:64542946-64542965 None:intergenic 50.0%
AGTGAGAGAGAGGATAAGGG+CGG + chr3.2:64543001-64543020 MS.gene82036:CDS 50.0%
CAGACACCAGAGGTTTCCAA+TGG + chr3.2:64542927-64542946 MS.gene82036:CDS 50.0%
CGTTGAGGTGAAGCTGATGA+TGG - chr3.2:64541783-64541802 None:intergenic 50.0%
GCAGATCCATTGGAAACCTC+TGG - chr3.2:64542936-64542955 None:intergenic 50.0%
GTGGTTACAGGTTGCGACTT+AGG - chr3.2:64542966-64542985 None:intergenic 50.0%
TGATTCTGCCTGAGCAGGAT+TGG - chr3.2:64542907-64542926 None:intergenic 50.0%
CAGCAAACGAGGGATGCCAA+AGG + chr3.2:64543169-64543188 MS.gene82036:CDS 55.0%
GAGGGATGCCAAAGGAAGGT+TGG + chr3.2:64543177-64543196 MS.gene82036:CDS 55.0%
GGCAGAATCACAGACACCAG+AGG + chr3.2:64542917-64542936 MS.gene82036:CDS 55.0%
GTCTGTGATTCTGCCTGAGC+AGG - chr3.2:64542912-64542931 None:intergenic 55.0%
! AGTGTGCTCTGAGTGGCCTT+CGG - chr3.2:64542729-64542748 None:intergenic 55.0%
! CGAAACGAGTGTGCTCTGAG+TGG - chr3.2:64542736-64542755 None:intergenic 55.0%
GGCCTTCGGGAAAGAAGCTC+CGG - chr3.2:64542715-64542734 None:intergenic 60.0%
TCTGGACTTCCAGAAGCCCC+TGG + chr3.2:64543131-64543150 MS.gene82036:CDS 60.0%
TGCCGGAGCTTCTTTCCCGA+AGG + chr3.2:64542710-64542729 MS.gene82036:CDS 60.0%
! GTGTGCTCTGAGTGGCCTTC+GGG - chr3.2:64542728-64542747 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 64541761 64543325 64541761 ID=MS.gene82036
chr3.2 mRNA 64541761 64543325 64541761 ID=MS.gene82036.t1;Parent=MS.gene82036
chr3.2 exon 64541761 64541802 64541761 ID=MS.gene82036.t1.exon1;Parent=MS.gene82036.t1
chr3.2 CDS 64541761 64541802 64541761 ID=cds.MS.gene82036.t1;Parent=MS.gene82036.t1
chr3.2 exon 64542672 64543325 64542672 ID=MS.gene82036.t1.exon2;Parent=MS.gene82036.t1
chr3.2 CDS 64542672 64543325 64542672 ID=cds.MS.gene82036.t1;Parent=MS.gene82036.t1
Gene Sequence

>MS.gene82036

ATGGCTATATCTCTAAATCCCATCATCAGCTTCACCTCAACGGTACGTTTTTCTATCTTCTCTTACTTTCTAGTATTCTCTAATCACTTTAGTTTATAGATTTAAGGTTATGGTAAGAAGAGTGTGTTAGTTATGGAAATTTACGTATTTGCTTGTAATTGATTCTTGGTTCTGCATACTAAACATTATTTGACTTGGGTTTTTTAGTAATTATATGCGTTGAGAAGCACATATATTTAACAAACTCTAACAACGTGTTATGTTAATCGGAAACAGTATTCCTGGGTGAACTTCTTATGATAGTAATTGTTCCAGTGAAATTGCTTTATGTTAATATAAAATGCGCTATATCTATGGTGCACATGTTAAGTAGGTGCTGTACTATGGAAAATTTTGTTTCTACCATTTGATCAGTAAGTCCTTCAATTGGATCAATTGAGATATGGTGAGATTTATTGATTCAATGGTAAAAAAACTCGTGTTTTACTTGTGCCCTTTAAATAGTCATATTTCCTTGTGGATCTTTGTTAAGTTCATGTTTTGTCAAAGTGCATTAAACCAAAGTTCCAGGTGGACTTTCTCTAGAATGTATGTAGTACTGTAATTCTGTGAAGACAAATTTATTGATTGTAGCTTTGCAGTTATGTTGTGATCGGTTCTTAAGTTATGTATTTGTTAAGAGAGTTGTTTGATGATGAAAATGTTGGTTTAGCTATTGATCAGATTCAGAGTCCCTTTTATATGTCAATTTTGGGATTATTACTCTGAAAAATAAAATTATCTTCTAATATGTAATAGGTTATTAATAACTGTTATTGTGTTTAACATCAATTCCTTCAATATAATTCAATTATGTTAATTTTAACTCTCATTTTTCTGATCACTCACACAAGTCTTTCATTATCCCTTAGAAGTTGGAAACAAACTATCACGATGCATCTAGGTATTCTGCCGGAGCTTCTTTCCCGAAGGCCACTCAGAGCACACTCGTTTCGATAACGTCGGGAACAAGAATGTTTGTCAAGGAGAAAAGATTTGGTCACCGTTTTTCAGTTGCAAATAGTGATCAACTTTCAACAGATGCTAGTAATAAGGATACTGGCAGGGCTGCAGATTCATCTGCCAATGATCAGTTAACATCTACCAATCCTGCTCAGGCAGAATCACAGACACCAGAGGTTTCCAATGGATCTGCAGCATCTCCTAAGTCGCAACCTGTAACCACACGATCATCTCAGAAAGTGAGAGAGAGGATAAGGGCGGCTCGAGTTCTTAACCAGTCCAAGGAACCAAAAACAGCTAAATCAGAAATGGGAAGTAGTGTGTTGGCTGCTTTTAAAGAAAGTGACAGAGGGAAAAAGAGGAGAAGATCTGGACTTCCAGAAGCCCCTGGTAATTTGTTTGATGACAGCAAACGAGGGATGCCAAAGGAAGGTTGGACTTTTGATTTTCCTGGTGGTTCTGATCTCTTTCTGATTATATTTTCTTTTGTCTTTATCAGCACAGTGATGTTTGGAACAACTTACATTGTGTGGAAAGTTGGTGCAATCCATTTTAATGACTAA

Protein sequence

>MS.gene82036.t1

MAISLNPIISFTSTKLETNYHDASRYSAGASFPKATQSTLVSITSGTRMFVKEKRFGHRFSVANSDQLSTDASNKDTGRAADSSANDQLTSTNPAQAESQTPEVSNGSAASPKSQPVTTRSSQKVRERIRAARVLNQSKEPKTAKSEMGSSVLAAFKESDRGKKRRRSGLPEAPGNLFDDSKRGMPKEGWTFDFPGGSDLFLIIFSFVFISTVMFGTTYIVWKVGAIHFND