AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050690


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050690.t1 MTR_7g118130 60.800 125 38 1 1 114 1 125 2.77e-34 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTCCAATTCATCCAATT+TGG 0.162116 7.4:-486959 None:intergenic
CGATCATTGGGGTCCCAAAT+TGG 0.282259 7.4:+486945 MS.gene050690:CDS
TTCTCCAATTCATCCAATTT+GGG 0.339109 7.4:-486958 None:intergenic
CACTCTGTATAAACGATCAT+TGG 0.347459 7.4:+486932 MS.gene050690:CDS
GGGTCCCAAATTGGATGAAT+TGG 0.357124 7.4:+486954 MS.gene050690:CDS
AGTGGGTATTCAGGCCAATA+TGG 0.382608 7.4:+486759 MS.gene050690:CDS
GCATCATCGAGTGGGTATTC+AGG 0.385134 7.4:+486750 MS.gene050690:CDS
AGGAGTCTCTTCCATGCGAC+TGG 0.407561 7.4:-486884 None:intergenic
ACTAATCGATGATTCCATAT+TGG 0.431208 7.4:-486773 None:intergenic
ACTCTGTATAAACGATCATT+GGG 0.435834 7.4:+486933 MS.gene050690:CDS
CTCCTCTGCGCCCAGTCGCA+TGG 0.454617 7.4:+486873 MS.gene050690:CDS
CTCAGCAGCACACGTTGTCT+TGG 0.473627 7.4:+486699 MS.gene050690:CDS
CCGCCGTGATGGTAGAGAAC+TGG 0.483151 7.4:-486725 None:intergenic
AGCAGCACACGTTGTCTTGG+CGG 0.509222 7.4:+486702 MS.gene050690:CDS
CGATGATGCCGCCGCCGTGA+TGG 0.511909 7.4:-486736 None:intergenic
CATGCGACTGGGCGCAGAGG+AGG 0.514470 7.4:-486872 None:intergenic
TAGTGGGTCAGATGTATTGT+GGG 0.536796 7.4:+486792 MS.gene050690:CDS
GGAGTCTCTTCCATGCGACT+GGG 0.557621 7.4:-486883 None:intergenic
ATCAAAGAGTATGTTCTGAA+AGG 0.563818 7.4:-486993 None:intergenic
TTCCATGCGACTGGGCGCAG+AGG 0.567243 7.4:-486875 None:intergenic
CGAACAATAACATCGGTATG+AGG 0.567548 7.4:-486907 None:intergenic
GTTCTCTACCATCACGGCGG+CGG 0.567815 7.4:+486728 MS.gene050690:CDS
AATATGGAATCATCGATTAG+TGG 0.570871 7.4:+486775 MS.gene050690:CDS
GATCAAGATCAAGATCAAGA+AGG 0.590735 7.4:+486843 MS.gene050690:CDS
ATATGGAATCATCGATTAGT+GGG 0.595651 7.4:+486776 MS.gene050690:CDS
ACGGCGGCGGCATCATCGAG+TGG 0.607654 7.4:+486741 MS.gene050690:CDS
AAAAGGAAAAGATGTGGTGT+TGG 0.611610 7.4:+486659 None:intergenic
AATGATCGTTTATACAGAGT+GGG 0.619057 7.4:-486930 None:intergenic
CAATGATCGTTTATACAGAG+TGG 0.623120 7.4:-486931 None:intergenic
CGGCGGCGGCATCATCGAGT+GGG 0.630862 7.4:+486742 MS.gene050690:CDS
CGGCCAGTTCTCTACCATCA+CGG 0.634232 7.4:+486722 MS.gene050690:CDS
TTAGTGGGTCAGATGTATTG+TGG 0.640580 7.4:+486791 MS.gene050690:CDS
ACAATAACATCGGTATGAGG+AGG 0.666832 7.4:-486904 None:intergenic
TGCTGCTGAGATGACAACGT+TGG 0.701374 7.4:-486686 None:intergenic
CTCTGTATAAACGATCATTG+GGG 0.729290 7.4:+486934 MS.gene050690:CDS
GAGTGGGCGAACAATAACAT+CGG 0.729674 7.4:-486914 None:intergenic
CCAGTTCTCTACCATCACGG+CGG 0.789166 7.4:+486725 MS.gene050690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATGATCGTTTATACAGAGT+GGG - chr7.4:486933-486952 None:intergenic 30.0%
ACTAATCGATGATTCCATAT+TGG - chr7.4:486776-486795 None:intergenic 30.0%
ACTCTGTATAAACGATCATT+GGG + chr7.4:486933-486952 MS.gene050690:CDS 30.0%
TTCTCCAATTCATCCAATTT+GGG - chr7.4:486961-486980 None:intergenic 30.0%
TTTCTCCAATTCATCCAATT+TGG - chr7.4:486962-486981 None:intergenic 30.0%
! AATATGGAATCATCGATTAG+TGG + chr7.4:486775-486794 MS.gene050690:CDS 30.0%
! ATATGGAATCATCGATTAGT+GGG + chr7.4:486776-486795 MS.gene050690:CDS 30.0%
CAATGATCGTTTATACAGAG+TGG - chr7.4:486934-486953 None:intergenic 35.0%
CACTCTGTATAAACGATCAT+TGG + chr7.4:486932-486951 MS.gene050690:CDS 35.0%
CTCTGTATAAACGATCATTG+GGG + chr7.4:486934-486953 MS.gene050690:CDS 35.0%
GATCAAGATCAAGATCAAGA+AGG + chr7.4:486843-486862 MS.gene050690:CDS 35.0%
ACAATAACATCGGTATGAGG+AGG - chr7.4:486907-486926 None:intergenic 40.0%
CGAACAATAACATCGGTATG+AGG - chr7.4:486910-486929 None:intergenic 40.0%
! TAGTGGGTCAGATGTATTGT+GGG + chr7.4:486792-486811 MS.gene050690:CDS 40.0%
! TTAGTGGGTCAGATGTATTG+TGG + chr7.4:486791-486810 MS.gene050690:CDS 40.0%
AGTGGGTATTCAGGCCAATA+TGG + chr7.4:486759-486778 MS.gene050690:CDS 45.0%
GAGTGGGCGAACAATAACAT+CGG - chr7.4:486917-486936 None:intergenic 45.0%
GGGTCCCAAATTGGATGAAT+TGG + chr7.4:486954-486973 MS.gene050690:CDS 45.0%
CGATCATTGGGGTCCCAAAT+TGG + chr7.4:486945-486964 MS.gene050690:CDS 50.0%
GCATCATCGAGTGGGTATTC+AGG + chr7.4:486750-486769 MS.gene050690:CDS 50.0%
TGCTGCTGAGATGACAACGT+TGG - chr7.4:486689-486708 None:intergenic 50.0%
AGCAGCACACGTTGTCTTGG+CGG + chr7.4:486702-486721 MS.gene050690:CDS 55.0%
AGGAGTCTCTTCCATGCGAC+TGG - chr7.4:486887-486906 None:intergenic 55.0%
CCAGTTCTCTACCATCACGG+CGG + chr7.4:486725-486744 MS.gene050690:CDS 55.0%
CGGCCAGTTCTCTACCATCA+CGG + chr7.4:486722-486741 MS.gene050690:CDS 55.0%
CTCAGCAGCACACGTTGTCT+TGG + chr7.4:486699-486718 MS.gene050690:CDS 55.0%
GGAGTCTCTTCCATGCGACT+GGG - chr7.4:486886-486905 None:intergenic 55.0%
CCGCCGTGATGGTAGAGAAC+TGG - chr7.4:486728-486747 None:intergenic 60.0%
GTTCTCTACCATCACGGCGG+CGG + chr7.4:486728-486747 MS.gene050690:CDS 60.0%
TTCCATGCGACTGGGCGCAG+AGG - chr7.4:486878-486897 None:intergenic 65.0%
ACGGCGGCGGCATCATCGAG+TGG + chr7.4:486741-486760 MS.gene050690:CDS 70.0%
CATGCGACTGGGCGCAGAGG+AGG - chr7.4:486875-486894 None:intergenic 70.0%
CGATGATGCCGCCGCCGTGA+TGG - chr7.4:486739-486758 None:intergenic 70.0%
CGGCGGCGGCATCATCGAGT+GGG + chr7.4:486742-486761 MS.gene050690:CDS 70.0%
CTCCTCTGCGCCCAGTCGCA+TGG + chr7.4:486873-486892 MS.gene050690:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 486670 487014 486670 ID=MS.gene050690
chr7.4 mRNA 486670 487014 486670 ID=MS.gene050690.t1;Parent=MS.gene050690
chr7.4 exon 486670 487014 486670 ID=MS.gene050690.t1.exon1;Parent=MS.gene050690.t1
chr7.4 CDS 486670 487014 486670 ID=cds.MS.gene050690.t1;Parent=MS.gene050690.t1
Gene Sequence

>MS.gene050690

ATGTGGTGTTGGTGTTCCAACGTTGTCATCTCAGCAGCACACGTTGTCTTGGCGGCCAGTTCTCTACCATCACGGCGGCGGCATCATCGAGTGGGTATTCAGGCCAATATGGAATCATCGATTAGTGGGTCAGATGTATTGTGGGCATTGCAGAAGAGAGCAAGCACTCGTAAGATCAAGATCAAGATCAAGAAGGAGTCATCCTCCTCTGCGCCCAGTCGCATGGAAGAGACTCCTCCTCATACCGATGTTATTGTTCGCCCACTCTGTATAAACGATCATTGGGGTCCCAAATTGGATGAATTGGAGAAACGTCTTCGTCACCTTTCAGAACATACTCTTTGA

Protein sequence

>MS.gene050690.t1

MWCWCSNVVISAAHVVLAASSLPSRRRHHRVGIQANMESSISGSDVLWALQKRASTRKIKIKIKKESSSSAPSRMEETPPHTDVIVRPLCINDHWGPKLDELEKRLRHLSEHTL