AlfalfaGEDB | MS.gene85119

M. sativa cultivar XinJiangDaYe / MS.gene85119

KEGG: K02961
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00366.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85119.t1	RHN76391.1	98.1	105	2	0	1	105	71	175	3.10E-49	204.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85119.t1	P17092	79.6	98	19	1	1	98	38	134	3.4e-36	152.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85119.t1	B7FMT1	98.1	105	2	0	1	105	47	151	2.2e-49	204.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene85119	0.855955	4.46E-62	-1.69E-46
MS.gene049173	MS.gene85119	0.837483	5.06E-57	-1.69E-46
MS.gene049217	MS.gene85119	0.840336	9.25E-58	-1.69E-46
MS.gene049222	MS.gene85119	0.84418	8.87E-59	-1.69E-46
MS.gene049232	MS.gene85119	0.847123	1.41E-59	-1.69E-46
MS.gene049285	MS.gene85119	0.887274	1.65E-72	-1.69E-46
MS.gene049320	MS.gene85119	0.871991	4.48E-67	-1.69E-46
MS.gene049627	MS.gene85119	0.826361	2.83E-54	-1.69E-46
MS.gene049633	MS.gene85119	0.801133	1.04E-48	-1.69E-46
MS.gene05010	MS.gene85119	0.836838	7.40E-57	-1.69E-46
MS.gene050163	MS.gene85119	0.906164	1.99E-80	-1.69E-46
MS.gene050165	MS.gene85119	0.921922	1.92E-88	-1.69E-46
MS.gene050323	MS.gene85119	0.810581	1.07E-50	-1.69E-46
MS.gene050333	MS.gene85119	0.887602	1.24E-72	-1.69E-46
MS.gene050444	MS.gene85119	0.80767	4.51E-50	-1.69E-46
MS.gene050496	MS.gene85119	0.823725	1.19E-53	-1.69E-46
MS.gene050504	MS.gene85119	0.891484	3.81E-74	-1.69E-46
MS.gene050518	MS.gene85119	0.883456	4.42E-71	-1.69E-46
MS.gene050577	MS.gene85119	0.82651	2.61E-54	-1.69E-46
MS.gene050612	MS.gene85119	0.88994	1.54E-73	-1.69E-46
MS.gene050675	MS.gene85119	0.852472	4.52E-61	-1.69E-46
MS.gene050699	MS.gene85119	0.863495	2.39E-64	-1.69E-46
MS.gene050772	MS.gene85119	0.814381	1.59E-51	-1.69E-46
MS.gene050832	MS.gene85119	-0.811394	7.16E-51	-1.69E-46
MS.gene050876	MS.gene85119	0.876142	1.77E-68	-1.69E-46
MS.gene05087	MS.gene85119	0.857385	1.69E-62	-1.69E-46
MS.gene050904	MS.gene85119	0.828189	1.03E-54	-1.69E-46
MS.gene050986	MS.gene85119	0.872383	3.32E-67	-1.69E-46
MS.gene05100	MS.gene85119	0.842795	2.08E-58	-1.69E-46
MS.gene051317	MS.gene85119	0.839632	1.41E-57	-1.69E-46
MS.gene051366	MS.gene85119	-0.810013	1.42E-50	-1.69E-46
MS.gene051551	MS.gene85119	0.839381	1.64E-57	-1.69E-46
MS.gene051810	MS.gene85119	0.857777	1.30E-62	-1.69E-46
MS.gene051949	MS.gene85119	0.870469	1.43E-66	-1.69E-46
MS.gene052482	MS.gene85119	0.802853	4.61E-49	-1.69E-46
MS.gene052486	MS.gene85119	0.812176	4.84E-51	-1.69E-46
MS.gene052916	MS.gene85119	0.820073	8.32E-53	-1.69E-46
MS.gene053203	MS.gene85119	0.811801	5.84E-51	-1.69E-46
MS.gene053213	MS.gene85119	0.833131	6.36E-56	-1.69E-46
MS.gene053284	MS.gene85119	0.804372	2.23E-49	-1.69E-46
MS.gene053286	MS.gene85119	0.833017	6.79E-56	-1.69E-46
MS.gene054600	MS.gene85119	0.806579	7.68E-50	-1.69E-46
MS.gene054638	MS.gene85119	0.828783	7.42E-55	-1.69E-46
MS.gene054642	MS.gene85119	0.867971	9.23E-66	-1.69E-46
MS.gene05470	MS.gene85119	0.835659	1.48E-56	-1.69E-46
MS.gene054954	MS.gene85119	0.901709	2.04E-78	-1.69E-46
MS.gene055204	MS.gene85119	-0.842194	3.00E-58	-1.69E-46
MS.gene055362	MS.gene85119	0.873829	1.09E-67	-1.69E-46
MS.gene055502	MS.gene85119	0.852697	3.89E-61	-1.69E-46
MS.gene055577	MS.gene85119	0.83139	1.71E-55	-1.69E-46
MS.gene055634	MS.gene85119	0.861014	1.38E-63	-1.69E-46
MS.gene055636	MS.gene85119	0.800166	1.64E-48	-1.69E-46
MS.gene055638	MS.gene85119	0.830068	3.62E-55	-1.69E-46
MS.gene055748	MS.gene85119	0.832424	9.53E-56	-1.69E-46
MS.gene055750	MS.gene85119	0.81155	6.62E-51	-1.69E-46
MS.gene055751	MS.gene85119	0.852268	5.16E-61	-1.69E-46
MS.gene055776	MS.gene85119	0.875928	2.09E-68	-1.69E-46
MS.gene055849	MS.gene85119	0.869857	2.26E-66	-1.69E-46
MS.gene05589	MS.gene85119	0.806488	8.03E-50	-1.69E-46
MS.gene056374	MS.gene85119	0.801564	8.49E-49	-1.69E-46
MS.gene056504	MS.gene85119	0.855694	5.31E-62	-1.69E-46
MS.gene056510	MS.gene85119	0.853822	1.85E-61	-1.69E-46
MS.gene056549	MS.gene85119	0.825837	3.77E-54	-1.69E-46
MS.gene056657	MS.gene85119	0.875245	3.59E-68	-1.69E-46
MS.gene056692	MS.gene85119	0.843927	1.04E-58	-1.69E-46
MS.gene056761	MS.gene85119	-0.813235	2.84E-51	-1.69E-46
MS.gene056898	MS.gene85119	0.806211	9.18E-50	-1.69E-46
MS.gene056910	MS.gene85119	0.823469	1.36E-53	-1.69E-46
MS.gene057148	MS.gene85119	0.849468	3.17E-60	-1.69E-46
MS.gene057831	MS.gene85119	0.823502	1.34E-53	-1.69E-46
MS.gene057986	MS.gene85119	0.844846	5.87E-59	-1.69E-46
MS.gene058334	MS.gene85119	-0.815435	9.26E-52	-1.69E-46
MS.gene058335	MS.gene85119	0.823068	1.69E-53	-1.69E-46
MS.gene058419	MS.gene85119	0.868193	7.83E-66	-1.69E-46
MS.gene058544	MS.gene85119	0.808268	3.37E-50	-1.69E-46
MS.gene058719	MS.gene85119	0.838502	2.77E-57	-1.69E-46
MS.gene058723	MS.gene85119	0.805201	1.50E-49	-1.69E-46
MS.gene058773	MS.gene85119	0.86028	2.31E-63	-1.69E-46
MS.gene059022	MS.gene85119	0.890969	6.09E-74	-1.69E-46
MS.gene059023	MS.gene85119	0.826207	3.08E-54	-1.69E-46
MS.gene059026	MS.gene85119	0.822452	2.35E-53	-1.69E-46
MS.gene059029	MS.gene85119	0.818203	2.22E-52	-1.69E-46
MS.gene059079	MS.gene85119	0.846438	2.17E-59	-1.69E-46
MS.gene05912	MS.gene85119	0.868404	6.69E-66	-1.69E-46
MS.gene059677	MS.gene85119	0.904041	1.86E-79	-1.69E-46
MS.gene059718	MS.gene85119	0.840523	8.26E-58	-1.69E-46
MS.gene059756	MS.gene85119	0.833345	5.63E-56	-1.69E-46
MS.gene059865	MS.gene85119	0.868554	5.99E-66	-1.69E-46
MS.gene060337	MS.gene85119	-0.850513	1.62E-60	-1.69E-46
MS.gene060703	MS.gene85119	0.905625	3.52E-80	-1.69E-46
MS.gene060762	MS.gene85119	0.86649	2.74E-65	-1.69E-46
MS.gene06077	MS.gene85119	0.848267	6.83E-60	-1.69E-46
MS.gene060823	MS.gene85119	-0.818217	2.20E-52	-1.69E-46
MS.gene060982	MS.gene85119	0.842645	2.28E-58	-1.69E-46
MS.gene061150	MS.gene85119	0.846704	1.84E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85119.t1	MTR_2g101770	98.095	105	2	0	1	105	47	151	6.34e-74	216

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85119.t1	AT1G79850	69.697	99	27	2	1	97	50	147	2.42e-44	141

Find 19 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAATACGTTTCTTGTATTT+AGG	0.176759	2.4:-3096519	None:intergenic
CCTGTTCCTGCTAGGAATTC+TGG	0.226525	2.4:+3096654	MS.gene85119:CDS
GGTAAGGCTGATAATTCTTC+TGG	0.342682	2.4:+3096675	MS.gene85119:CDS
AGAAGAACAGCCTCAGATTC+AGG	0.344474	2.4:+3096728	MS.gene85119:CDS
CTAAATACAAGAAACGTATT+AGG	0.362043	2.4:+3096520	MS.gene85119:CDS
TTGCTGTTCCTGTTCCTGCT+AGG	0.444869	2.4:+3096646	MS.gene85119:CDS
CCAGAATTCCTAGCAGGAAC+AGG	0.463154	2.4:-3096654	None:intergenic
TAGGATGAAGAAGAAGTATC+AGG	0.470403	2.4:+3096539	MS.gene85119:CDS
CGTTTCTTGTATTTAGGATG+AGG	0.484431	2.4:-3096513	None:intergenic
CCTCGACATTCCGCTTCAAT+CGG	0.508100	2.4:+3096701	MS.gene85119:CDS
TCCTGCTAGGAATTCTGGTA+AGG	0.524466	2.4:+3096659	MS.gene85119:CDS
TCTTCTTGCTCCGATTGAAG+CGG	0.531289	2.4:-3096711	None:intergenic
GCCTTACCAGAATTCCTAGC+AGG	0.562008	2.4:-3096660	None:intergenic
CAAAACTAAGCCTGAATCTG+AGG	0.563443	2.4:-3096738	None:intergenic
AATCAAAGCTATGAAAACAA+TGG	0.574259	2.4:+3096428	None:intergenic
CAACGACAAAACCGTGTCTG+TGG	0.582090	2.4:+3096473	MS.gene85119:CDS
AAAGCTATGAAAACAATGGA+AGG	0.583695	2.4:+3096432	None:intergenic
ACGAGTTACTTCCACAGACA+CGG	0.603802	2.4:-3096484	None:intergenic
CCGATTGAAGCGGAATGTCG+AGG	0.647031	2.4:-3096701	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAGTTTTATTCTTACTAAT+AGG	-	chr2.4:3096624-3096643	None:intergenic	15.0%
!	CTAAATACAAGAAACGTATT+AGG	+	chr2.4:3096520-3096539	MS.gene85119:CDS	25.0%
!	CTAATACGTTTCTTGTATTT+AGG	-	chr2.4:3096522-3096541	None:intergenic	25.0%
!	CCTGAAAACGTTTTCAAAGT+TGG	+	chr2.4:3096570-3096589	MS.gene85119:CDS	35.0%
!	CGTTTCTTGTATTTAGGATG+AGG	-	chr2.4:3096516-3096535	None:intergenic	35.0%
!!	CCAACTTTGAAAACGTTTTC+AGG	-	chr2.4:3096573-3096592	None:intergenic	35.0%
!!	TAGGATGAAGAAGAAGTATC+AGG	+	chr2.4:3096539-3096558	MS.gene85119:CDS	35.0%
	GGTAAGGCTGATAATTCTTC+TGG	+	chr2.4:3096675-3096694	MS.gene85119:CDS	40.0%
	ACGAGTTACTTCCACAGACA+CGG	-	chr2.4:3096487-3096506	None:intergenic	45.0%
	AGAAGAACAGCCTCAGATTC+AGG	+	chr2.4:3096728-3096747	MS.gene85119:CDS	45.0%
	TCCTGCTAGGAATTCTGGTA+AGG	+	chr2.4:3096659-3096678	MS.gene85119:CDS	45.0%
	TCTTCTTGCTCCGATTGAAG+CGG	-	chr2.4:3096714-3096733	None:intergenic	45.0%
	CAACGACAAAACCGTGTCTG+TGG	+	chr2.4:3096473-3096492	MS.gene85119:CDS	50.0%
	CCAGAATTCCTAGCAGGAAC+AGG	-	chr2.4:3096657-3096676	None:intergenic	50.0%
	CCTCGACATTCCGCTTCAAT+CGG	+	chr2.4:3096701-3096720	MS.gene85119:CDS	50.0%
	CCTGTTCCTGCTAGGAATTC+TGG	+	chr2.4:3096654-3096673	MS.gene85119:CDS	50.0%
	GCCTTACCAGAATTCCTAGC+AGG	-	chr2.4:3096663-3096682	None:intergenic	50.0%
!	TTGCTGTTCCTGTTCCTGCT+AGG	+	chr2.4:3096646-3096665	MS.gene85119:CDS	50.0%
!!	AGACACGGTTTTGTCGTTGG+TGG	-	chr2.4:3096472-3096491	None:intergenic	50.0%
!!	CACAGACACGGTTTTGTCGT+TGG	-	chr2.4:3096475-3096494	None:intergenic	50.0%
	CCGATTGAAGCGGAATGTCG+AGG	-	chr2.4:3096704-3096723	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	3096438	3096755	3096438	ID=MS.gene85119
chr2.4	mRNA	3096438	3096755	3096438	ID=MS.gene85119.t1;Parent=MS.gene85119
chr2.4	exon	3096438	3096755	3096438	ID=MS.gene85119.t1.exon1;Parent=MS.gene85119.t1
chr2.4	CDS	3096438	3096755	3096438	ID=cds.MS.gene85119.t1;Parent=MS.gene85119.t1

Gene Sequence	>MS.gene85119 ATGAAAACAATGGAAGGAAAAGTTGTGTGTGCCACCAACGACAAAACCGTGTCTGTGGAAGTAACTCGTTTAGCACCTCATCCTAAATACAAGAAACGTATTAGGATGAAGAAGAAGTATCAGGCTCATGATCCTGAAAACGTTTTCAAAGTTGGTGATGTTGTTCAGCTTTGTAAAATTAGACCTATTAGTAAGAATAAAACTTTTCTTGCTGTTCCTGTTCCTGCTAGGAATTCTGGTAAGGCTGATAATTCTTCTGGTGACCTCGACATTCCGCTTCAATCGGAGCAAGAAGAACAGCCTCAGATTCAGGCTTAG
Protein sequence	>MS.gene85119.t1 MKTMEGKVVCATNDKTVSVEVTRLAPHPKYKKRIRMKKKYQAHDPENVFKVGDVVQLCKIRPISKNKTFLAVPVPARNSGKADNSSGDLDIPLQSEQEEQPQIQA