Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86294.t1 | KEH39767.1 | 91.6 | 83 | 7 | 0 | 1 | 83 | 1 | 83 | 1.10E-33 | 152.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86294.t1 | Q8RWG1 | 60.9 | 46 | 14 | 1 | 38 | 83 | 78 | 119 | 1.3e-06 | 53.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86294.t1 | A0A072VE85 | 91.6 | 83 | 7 | 0 | 1 | 83 | 1 | 83 | 8.2e-34 | 152.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049222 | MS.gene86294 | 0.819179 | 1.33E-52 | -1.69E-46 |
| MS.gene049232 | MS.gene86294 | 0.85295 | 3.30E-61 | -1.69E-46 |
| MS.gene049369 | MS.gene86294 | 0.825148 | 5.49E-54 | -1.69E-46 |
| MS.gene049396 | MS.gene86294 | 0.866865 | 2.09E-65 | -1.69E-46 |
| MS.gene049552 | MS.gene86294 | 0.809052 | 2.29E-50 | -1.69E-46 |
| MS.gene049633 | MS.gene86294 | 0.813119 | 3.01E-51 | -1.69E-46 |
| MS.gene049656 | MS.gene86294 | 0.802943 | 4.41E-49 | -1.69E-46 |
| MS.gene05010 | MS.gene86294 | 0.804709 | 1.90E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene86294 | 0.842883 | 1.97E-58 | -1.69E-46 |
| MS.gene050323 | MS.gene86294 | 0.84359 | 1.28E-58 | -1.69E-46 |
| MS.gene050496 | MS.gene86294 | 0.816547 | 5.23E-52 | -1.69E-46 |
| MS.gene050499 | MS.gene86294 | 0.820273 | 7.48E-53 | -1.69E-46 |
| MS.gene050502 | MS.gene86294 | 0.863784 | 1.94E-64 | -1.69E-46 |
| MS.gene050504 | MS.gene86294 | 0.80893 | 2.43E-50 | -1.69E-46 |
| MS.gene050518 | MS.gene86294 | 0.801989 | 6.94E-49 | -1.69E-46 |
| MS.gene050520 | MS.gene86294 | 0.810462 | 1.14E-50 | -1.69E-46 |
| MS.gene050577 | MS.gene86294 | 0.811116 | 8.22E-51 | -1.69E-46 |
| MS.gene050675 | MS.gene86294 | 0.82918 | 5.94E-55 | -1.69E-46 |
| MS.gene050697 | MS.gene86294 | 0.851275 | 9.87E-61 | -1.69E-46 |
| MS.gene050747 | MS.gene86294 | 0.822283 | 2.57E-53 | -1.69E-46 |
| MS.gene050772 | MS.gene86294 | 0.814665 | 1.37E-51 | -1.69E-46 |
| MS.gene05080 | MS.gene86294 | 0.827904 | 1.21E-54 | -1.69E-46 |
| MS.gene050868 | MS.gene86294 | 0.834353 | 3.15E-56 | -1.69E-46 |
| MS.gene050871 | MS.gene86294 | 0.838998 | 2.06E-57 | -1.69E-46 |
| MS.gene050904 | MS.gene86294 | 0.803898 | 2.80E-49 | -1.69E-46 |
| MS.gene050950 | MS.gene86294 | 0.824064 | 9.88E-54 | -1.69E-46 |
| MS.gene050986 | MS.gene86294 | 0.822122 | 2.80E-53 | -1.69E-46 |
| MS.gene05118 | MS.gene86294 | 0.807874 | 4.08E-50 | -1.69E-46 |
| MS.gene051268 | MS.gene86294 | 0.816135 | 6.46E-52 | -1.69E-46 |
| MS.gene051509 | MS.gene86294 | 0.87004 | 1.97E-66 | -1.69E-46 |
| MS.gene051734 | MS.gene86294 | 0.819171 | 1.34E-52 | -1.69E-46 |
| MS.gene051838 | MS.gene86294 | 0.810986 | 8.77E-51 | -1.69E-46 |
| MS.gene051874 | MS.gene86294 | 0.803743 | 3.01E-49 | -1.69E-46 |
| MS.gene051941 | MS.gene86294 | 0.872067 | 4.23E-67 | -1.69E-46 |
| MS.gene052066 | MS.gene86294 | 0.802032 | 6.80E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene86294 | 0.845158 | 4.84E-59 | -1.69E-46 |
| MS.gene052346 | MS.gene86294 | 0.801267 | 9.76E-49 | -1.69E-46 |
| MS.gene052414 | MS.gene86294 | 0.820154 | 7.97E-53 | -1.69E-46 |
| MS.gene052482 | MS.gene86294 | 0.823741 | 1.18E-53 | -1.69E-46 |
| MS.gene052486 | MS.gene86294 | 0.828632 | 8.07E-55 | -1.69E-46 |
| MS.gene052503 | MS.gene86294 | 0.824102 | 9.68E-54 | -1.69E-46 |
| MS.gene052615 | MS.gene86294 | 0.801739 | 7.81E-49 | -1.69E-46 |
| MS.gene052823 | MS.gene86294 | 0.824574 | 7.50E-54 | -1.69E-46 |
| MS.gene052825 | MS.gene86294 | 0.810624 | 1.05E-50 | -1.69E-46 |
| MS.gene052966 | MS.gene86294 | 0.810277 | 1.25E-50 | -1.69E-46 |
| MS.gene053203 | MS.gene86294 | 0.820283 | 7.44E-53 | -1.69E-46 |
| MS.gene053213 | MS.gene86294 | 0.801492 | 8.78E-49 | -1.69E-46 |
| MS.gene053436 | MS.gene86294 | 0.827186 | 1.80E-54 | -1.69E-46 |
| MS.gene053480 | MS.gene86294 | 0.818926 | 1.52E-52 | -1.69E-46 |
| MS.gene053505 | MS.gene86294 | 0.818498 | 1.90E-52 | -1.69E-46 |
| MS.gene053574 | MS.gene86294 | 0.854143 | 1.50E-61 | -1.69E-46 |
| MS.gene053626 | MS.gene86294 | 0.86673 | 2.30E-65 | -1.69E-46 |
| MS.gene053633 | MS.gene86294 | 0.803031 | 4.23E-49 | -1.69E-46 |
| MS.gene053824 | MS.gene86294 | 0.809742 | 1.63E-50 | -1.69E-46 |
| MS.gene053912 | MS.gene86294 | 0.817482 | 3.22E-52 | -1.69E-46 |
| MS.gene054003 | MS.gene86294 | 0.802688 | 4.98E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene86294 | 0.844325 | 8.11E-59 | -1.69E-46 |
| MS.gene054706 | MS.gene86294 | -0.803815 | 2.91E-49 | -1.69E-46 |
| MS.gene05470 | MS.gene86294 | 0.801207 | 1.00E-48 | -1.69E-46 |
| MS.gene05494 | MS.gene86294 | 0.806659 | 7.39E-50 | -1.69E-46 |
| MS.gene054954 | MS.gene86294 | 0.803854 | 2.86E-49 | -1.69E-46 |
| MS.gene05505 | MS.gene86294 | 0.802951 | 4.40E-49 | -1.69E-46 |
| MS.gene055224 | MS.gene86294 | 0.806995 | 6.28E-50 | -1.69E-46 |
| MS.gene055629 | MS.gene86294 | 0.807738 | 4.37E-50 | -1.69E-46 |
| MS.gene055741 | MS.gene86294 | 0.847802 | 9.18E-60 | -1.69E-46 |
| MS.gene055744 | MS.gene86294 | 0.839462 | 1.56E-57 | -1.69E-46 |
| MS.gene055750 | MS.gene86294 | 0.822902 | 1.85E-53 | -1.69E-46 |
| MS.gene05589 | MS.gene86294 | 0.84707 | 1.46E-59 | -1.69E-46 |
| MS.gene055909 | MS.gene86294 | 0.859034 | 5.47E-63 | -1.69E-46 |
| MS.gene055911 | MS.gene86294 | 0.857741 | 1.33E-62 | -1.69E-46 |
| MS.gene055941 | MS.gene86294 | 0.802789 | 4.75E-49 | -1.69E-46 |
| MS.gene05601 | MS.gene86294 | 0.801152 | 1.03E-48 | -1.69E-46 |
| MS.gene056040 | MS.gene86294 | 0.83972 | 1.34E-57 | -1.69E-46 |
| MS.gene056166 | MS.gene86294 | 0.88001 | 7.81E-70 | -1.69E-46 |
| MS.gene056387 | MS.gene86294 | 0.850641 | 1.49E-60 | -1.69E-46 |
| MS.gene056422 | MS.gene86294 | 0.83152 | 1.59E-55 | -1.69E-46 |
| MS.gene056446 | MS.gene86294 | 0.813284 | 2.77E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene86294 | 0.853606 | 2.14E-61 | -1.69E-46 |
| MS.gene056510 | MS.gene86294 | 0.8085 | 3.00E-50 | -1.69E-46 |
| MS.gene056511 | MS.gene86294 | 0.800583 | 1.35E-48 | -1.69E-46 |
| MS.gene056657 | MS.gene86294 | 0.808111 | 3.64E-50 | -1.69E-46 |
| MS.gene05666 | MS.gene86294 | 0.816657 | 4.94E-52 | -1.69E-46 |
| MS.gene057019 | MS.gene86294 | 0.854293 | 1.36E-61 | -1.69E-46 |
| MS.gene057076 | MS.gene86294 | 0.843529 | 1.32E-58 | -1.69E-46 |
| MS.gene057086 | MS.gene86294 | 0.838 | 3.73E-57 | -1.69E-46 |
| MS.gene057734 | MS.gene86294 | 0.838895 | 2.19E-57 | -1.69E-46 |
| MS.gene057831 | MS.gene86294 | 0.800444 | 1.44E-48 | -1.69E-46 |
| MS.gene057876 | MS.gene86294 | 0.823613 | 1.26E-53 | -1.69E-46 |
| MS.gene05787 | MS.gene86294 | 0.887429 | 1.44E-72 | -1.69E-46 |
| MS.gene058075 | MS.gene86294 | 0.810653 | 1.04E-50 | -1.69E-46 |
| MS.gene058090 | MS.gene86294 | 0.808238 | 3.42E-50 | -1.69E-46 |
| MS.gene058120 | MS.gene86294 | 0.844911 | 5.64E-59 | -1.69E-46 |
| MS.gene058130 | MS.gene86294 | 0.812119 | 4.98E-51 | -1.69E-46 |
| MS.gene058157 | MS.gene86294 | 0.801594 | 8.36E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene86294 | 0.814491 | 1.50E-51 | -1.69E-46 |
| MS.gene058948 | MS.gene86294 | 0.815333 | 9.75E-52 | -1.69E-46 |
| MS.gene05913 | MS.gene86294 | 0.803076 | 4.14E-49 | -1.69E-46 |
| MS.gene059305 | MS.gene86294 | 0.810418 | 1.16E-50 | -1.69E-46 |
| MS.gene059367 | MS.gene86294 | 0.872424 | 3.22E-67 | -1.69E-46 |
| MS.gene059368 | MS.gene86294 | 0.85096 | 1.21E-60 | -1.69E-46 |
| MS.gene059455 | MS.gene86294 | 0.809935 | 1.48E-50 | -1.69E-46 |
| MS.gene059517 | MS.gene86294 | 0.805282 | 1.44E-49 | -1.69E-46 |
| MS.gene059667 | MS.gene86294 | 0.838874 | 2.22E-57 | -1.69E-46 |
| MS.gene059674 | MS.gene86294 | 0.816366 | 5.74E-52 | -1.69E-46 |
| MS.gene059680 | MS.gene86294 | 0.846262 | 2.43E-59 | -1.69E-46 |
| MS.gene059865 | MS.gene86294 | 0.844664 | 6.57E-59 | -1.69E-46 |
| MS.gene05995 | MS.gene86294 | 0.805239 | 1.47E-49 | -1.69E-46 |
| MS.gene06000 | MS.gene86294 | 0.854132 | 1.51E-61 | -1.69E-46 |
| MS.gene060372 | MS.gene86294 | 0.8615 | 9.82E-64 | -1.69E-46 |
| MS.gene060482 | MS.gene86294 | 0.80228 | 6.05E-49 | -1.69E-46 |
| MS.gene060595 | MS.gene86294 | -0.801972 | 7.00E-49 | -1.69E-46 |
| MS.gene060778 | MS.gene86294 | 0.88357 | 4.01E-71 | -1.69E-46 |
| MS.gene06077 | MS.gene86294 | 0.814457 | 1.53E-51 | -1.69E-46 |
| MS.gene060835 | MS.gene86294 | 0.838549 | 2.69E-57 | -1.69E-46 |
| MS.gene06097 | MS.gene86294 | 0.859266 | 4.66E-63 | -1.69E-46 |
| MS.gene060982 | MS.gene86294 | 0.849494 | 3.12E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86294.t1 | MTR_1g009860 | 91.566 | 83 | 7 | 0 | 1 | 83 | 1 | 83 | 3.40e-46 | 155 |
| MS.gene86294.t1 | MTR_1g009860 | 91.566 | 83 | 7 | 0 | 1 | 83 | 1 | 83 | 3.44e-46 | 156 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGAGTGCGAATTTCGAATT+TGG | 0.238449 | 1.3:+10384317 | MS.gene86294:CDS |
| CTATTTCGCCGGGAAGTAAT+CGG | 0.364776 | 1.3:+10384283 | MS.gene86294:CDS |
| TCGAATTTGGCGTCGACGAC+GGG | 0.372561 | 1.3:+10384330 | MS.gene86294:CDS |
| ACATTCTTCCGATTACTTCC+CGG | 0.401889 | 1.3:-10384291 | None:intergenic |
| TTGGCGTCGACGACGGGAAA+CGG | 0.455624 | 1.3:+10384336 | MS.gene86294:CDS |
| TCCTCAATTGACTATTTCGC+CGG | 0.488550 | 1.3:+10384272 | MS.gene86294:CDS |
| TTCGAATTTGGCGTCGACGA+CGG | 0.501571 | 1.3:+10384329 | MS.gene86294:CDS |
| CCTCAATTGACTATTTCGCC+GGG | 0.561696 | 1.3:+10384273 | MS.gene86294:CDS |
| CAATCTCAGGTGAGTACTTG+CGG | 0.570802 | 1.3:-10384455 | None:intergenic |
| GAAGTAATCGGAAGAATGTA+CGG | 0.573623 | 1.3:+10384295 | MS.gene86294:CDS |
| GTTGACGCAGAAGGATTCCA+TGG | 0.577555 | 1.3:-10384232 | None:intergenic |
| GAATTACTCGGCAGCTGCAA+CGG | 0.582790 | 1.3:-10384372 | None:intergenic |
| TTATTCGACTTTGAATTACT+CGG | 0.586429 | 1.3:-10384384 | None:intergenic |
| AAAGTCGAATAATAGTGCGT+TGG | 0.608667 | 1.3:+10384395 | MS.gene86294:CDS |
| TCAGGTGAGTACTTGCGGAA+AGG | 0.614975 | 1.3:-10384450 | None:intergenic |
| CGGGAAACGGTGCTGTTGTG+AGG | 0.619284 | 1.3:+10384349 | MS.gene86294:CDS |
| CCCGGCGAAATAGTCAATTG+AGG | 0.628438 | 1.3:-10384273 | None:intergenic |
| GTACAAAGAGTTGACGCAGA+AGG | 0.631834 | 1.3:-10384241 | None:intergenic |
| GAGCAGCTTGATATTGAGCG+TGG | 0.673845 | 1.3:+10384417 | MS.gene86294:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAAAAAAAACGTACAATCTC+AGG | - | chr1.3:10384471-10384490 | None:intergenic | 25.0% |
| ! | TTATTCGACTTTGAATTACT+CGG | - | chr1.3:10384387-10384406 | None:intergenic | 25.0% |
| AAAGTCGAATAATAGTGCGT+TGG | + | chr1.3:10384395-10384414 | MS.gene86294:CDS | 35.0% | |
| GAAGTAATCGGAAGAATGTA+CGG | + | chr1.3:10384295-10384314 | MS.gene86294:CDS | 35.0% | |
| ACATTCTTCCGATTACTTCC+CGG | - | chr1.3:10384294-10384313 | None:intergenic | 40.0% | |
| TCCTCAATTGACTATTTCGC+CGG | + | chr1.3:10384272-10384291 | MS.gene86294:CDS | 40.0% | |
| CAATCTCAGGTGAGTACTTG+CGG | - | chr1.3:10384458-10384477 | None:intergenic | 45.0% | |
| CCTCAATTGACTATTTCGCC+GGG | + | chr1.3:10384273-10384292 | MS.gene86294:CDS | 45.0% | |
| CTATTTCGCCGGGAAGTAAT+CGG | + | chr1.3:10384283-10384302 | MS.gene86294:CDS | 45.0% | |
| GCGAGTGCGAATTTCGAATT+TGG | + | chr1.3:10384317-10384336 | MS.gene86294:CDS | 45.0% | |
| GTACAAAGAGTTGACGCAGA+AGG | - | chr1.3:10384244-10384263 | None:intergenic | 45.0% | |
| CCCGGCGAAATAGTCAATTG+AGG | - | chr1.3:10384276-10384295 | None:intergenic | 50.0% | |
| GAATTACTCGGCAGCTGCAA+CGG | - | chr1.3:10384375-10384394 | None:intergenic | 50.0% | |
| GAGCAGCTTGATATTGAGCG+TGG | + | chr1.3:10384417-10384436 | MS.gene86294:CDS | 50.0% | |
| TCAGGTGAGTACTTGCGGAA+AGG | - | chr1.3:10384453-10384472 | None:intergenic | 50.0% | |
| !! | TTCGAATTTGGCGTCGACGA+CGG | + | chr1.3:10384329-10384348 | MS.gene86294:CDS | 50.0% |
| !! | TCGAATTTGGCGTCGACGAC+GGG | + | chr1.3:10384330-10384349 | MS.gene86294:CDS | 55.0% |
| !! | CGGGAAACGGTGCTGTTGTG+AGG | + | chr1.3:10384349-10384368 | MS.gene86294:CDS | 60.0% |
| !! | TTGGCGTCGACGACGGGAAA+CGG | + | chr1.3:10384336-10384355 | MS.gene86294:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 10384234 | 10384491 | 10384234 | ID=MS.gene86294 |
| chr1.3 | mRNA | 10384234 | 10384491 | 10384234 | ID=MS.gene86294.t1;Parent=MS.gene86294 |
| chr1.3 | exon | 10384234 | 10384491 | 10384234 | ID=MS.gene86294.t1.exon1;Parent=MS.gene86294.t1 |
| chr1.3 | CDS | 10384234 | 10384491 | 10384234 | ID=cds.MS.gene86294.t1;Parent=MS.gene86294.t1 |
| Gene Sequence |
| Protein sequence |
