Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92718.t1 | PNY09752.1 | 85.7 | 105 | 15 | 0 | 1 | 105 | 1 | 105 | 6.30E-42 | 179.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92718.t1 | P31336 | 56.1 | 107 | 43 | 3 | 1 | 105 | 1 | 105 | 3.7e-19 | 95.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92718.t1 | A0A2K3P381 | 85.7 | 105 | 15 | 0 | 1 | 105 | 1 | 105 | 4.5e-42 | 179.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049351 | MS.gene92718 | 0.808722 | 2.69E-50 | -1.69E-46 |
| MS.gene049356 | MS.gene92718 | 0.80154 | 8.58E-49 | -1.69E-46 |
| MS.gene049427 | MS.gene92718 | 0.805052 | 1.61E-49 | -1.69E-46 |
| MS.gene049491 | MS.gene92718 | 0.810942 | 8.97E-51 | -1.69E-46 |
| MS.gene049614 | MS.gene92718 | 0.807367 | 5.23E-50 | -1.69E-46 |
| MS.gene049661 | MS.gene92718 | 0.813843 | 2.08E-51 | -1.69E-46 |
| MS.gene049662 | MS.gene92718 | 0.816127 | 6.49E-52 | -1.69E-46 |
| MS.gene049758 | MS.gene92718 | 0.830864 | 2.31E-55 | -1.69E-46 |
| MS.gene050076 | MS.gene92718 | 0.844452 | 7.49E-59 | -1.69E-46 |
| MS.gene050099 | MS.gene92718 | 0.808523 | 2.97E-50 | -1.69E-46 |
| MS.gene050128 | MS.gene92718 | 0.822068 | 2.89E-53 | -1.69E-46 |
| MS.gene050301 | MS.gene92718 | 0.846033 | 2.80E-59 | -1.69E-46 |
| MS.gene050397 | MS.gene92718 | 0.817622 | 3.00E-52 | -1.69E-46 |
| MS.gene050432 | MS.gene92718 | 0.873014 | 2.04E-67 | -1.69E-46 |
| MS.gene050529 | MS.gene92718 | 0.804874 | 1.75E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene92718 | 0.810675 | 1.02E-50 | -1.69E-46 |
| MS.gene050767 | MS.gene92718 | 0.885745 | 6.24E-72 | -1.69E-46 |
| MS.gene050770 | MS.gene92718 | 0.884625 | 1.63E-71 | -1.69E-46 |
| MS.gene05101 | MS.gene92718 | 0.856098 | 4.05E-62 | -1.69E-46 |
| MS.gene05105 | MS.gene92718 | 0.838906 | 2.18E-57 | -1.69E-46 |
| MS.gene051228 | MS.gene92718 | 0.818169 | 2.26E-52 | -1.69E-46 |
| MS.gene051387 | MS.gene92718 | 0.804656 | 1.95E-49 | -1.69E-46 |
| MS.gene051471 | MS.gene92718 | 0.840131 | 1.05E-57 | -1.69E-46 |
| MS.gene052078 | MS.gene92718 | 0.844908 | 5.65E-59 | -1.69E-46 |
| MS.gene052178 | MS.gene92718 | 0.839188 | 1.84E-57 | -1.69E-46 |
| MS.gene052188 | MS.gene92718 | 0.821588 | 3.73E-53 | -1.69E-46 |
| MS.gene052271 | MS.gene92718 | 0.81203 | 5.20E-51 | -1.69E-46 |
| MS.gene052290 | MS.gene92718 | 0.821949 | 3.08E-53 | -1.69E-46 |
| MS.gene052310 | MS.gene92718 | 0.858256 | 9.34E-63 | -1.69E-46 |
| MS.gene052326 | MS.gene92718 | 0.812355 | 4.42E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene92718 | 0.837912 | 3.93E-57 | -1.69E-46 |
| MS.gene052421 | MS.gene92718 | 0.80827 | 3.36E-50 | -1.69E-46 |
| MS.gene052484 | MS.gene92718 | 0.806577 | 7.69E-50 | -1.69E-46 |
| MS.gene052485 | MS.gene92718 | 0.818718 | 1.69E-52 | -1.69E-46 |
| MS.gene052508 | MS.gene92718 | 0.807167 | 5.77E-50 | -1.69E-46 |
| MS.gene053009 | MS.gene92718 | 0.816757 | 4.69E-52 | -1.69E-46 |
| MS.gene053103 | MS.gene92718 | 0.831382 | 1.72E-55 | -1.69E-46 |
| MS.gene053104 | MS.gene92718 | 0.820095 | 8.22E-53 | -1.69E-46 |
| MS.gene053209 | MS.gene92718 | 0.887534 | 1.31E-72 | -1.69E-46 |
| MS.gene053322 | MS.gene92718 | 0.827306 | 1.68E-54 | -1.69E-46 |
| MS.gene053449 | MS.gene92718 | 0.803274 | 3.77E-49 | -1.69E-46 |
| MS.gene053557 | MS.gene92718 | 0.833905 | 4.08E-56 | -1.69E-46 |
| MS.gene053813 | MS.gene92718 | 0.820369 | 7.11E-53 | -1.69E-46 |
| MS.gene053928 | MS.gene92718 | 0.811128 | 8.17E-51 | -1.69E-46 |
| MS.gene054123 | MS.gene92718 | 0.803076 | 4.14E-49 | -1.69E-46 |
| MS.gene054376 | MS.gene92718 | 0.884248 | 2.25E-71 | -1.69E-46 |
| MS.gene054802 | MS.gene92718 | 0.829862 | 4.06E-55 | -1.69E-46 |
| MS.gene054803 | MS.gene92718 | 0.831929 | 1.26E-55 | -1.69E-46 |
| MS.gene054804 | MS.gene92718 | 0.834224 | 3.39E-56 | -1.69E-46 |
| MS.gene05505 | MS.gene92718 | 0.806344 | 8.61E-50 | -1.69E-46 |
| MS.gene055116 | MS.gene92718 | 0.837575 | 4.79E-57 | -1.69E-46 |
| MS.gene055254 | MS.gene92718 | 0.838257 | 3.20E-57 | -1.69E-46 |
| MS.gene055277 | MS.gene92718 | 0.874826 | 4.98E-68 | -1.69E-46 |
| MS.gene055390 | MS.gene92718 | 0.820946 | 5.24E-53 | -1.69E-46 |
| MS.gene055397 | MS.gene92718 | 0.819181 | 1.33E-52 | -1.69E-46 |
| MS.gene055412 | MS.gene92718 | 0.831096 | 2.03E-55 | -1.69E-46 |
| MS.gene055422 | MS.gene92718 | 0.838697 | 2.47E-57 | -1.69E-46 |
| MS.gene055428 | MS.gene92718 | 0.817004 | 4.13E-52 | -1.69E-46 |
| MS.gene055497 | MS.gene92718 | -0.804597 | 2.00E-49 | -1.69E-46 |
| MS.gene055499 | MS.gene92718 | 0.821249 | 4.46E-53 | -1.69E-46 |
| MS.gene055647 | MS.gene92718 | 0.817951 | 2.53E-52 | -1.69E-46 |
| MS.gene055722 | MS.gene92718 | 0.800785 | 1.22E-48 | -1.69E-46 |
| MS.gene05592 | MS.gene92718 | 0.874623 | 5.84E-68 | -1.69E-46 |
| MS.gene056191 | MS.gene92718 | 0.817167 | 3.80E-52 | -1.69E-46 |
| MS.gene056407 | MS.gene92718 | 0.84059 | 7.93E-58 | -1.69E-46 |
| MS.gene056410 | MS.gene92718 | 0.81633 | 5.85E-52 | -1.69E-46 |
| MS.gene056609 | MS.gene92718 | 0.84 | 1.13E-57 | -1.69E-46 |
| MS.gene056612 | MS.gene92718 | 0.802183 | 6.33E-49 | -1.69E-46 |
| MS.gene056897 | MS.gene92718 | 0.807415 | 5.11E-50 | -1.69E-46 |
| MS.gene057011 | MS.gene92718 | 0.826135 | 3.20E-54 | -1.69E-46 |
| MS.gene05702 | MS.gene92718 | 0.831299 | 1.81E-55 | -1.69E-46 |
| MS.gene057124 | MS.gene92718 | 0.827195 | 1.79E-54 | -1.69E-46 |
| MS.gene057129 | MS.gene92718 | 0.846681 | 1.86E-59 | -1.69E-46 |
| MS.gene057331 | MS.gene92718 | 0.841969 | 3.44E-58 | -1.69E-46 |
| MS.gene05735 | MS.gene92718 | 0.813997 | 1.93E-51 | -1.69E-46 |
| MS.gene057416 | MS.gene92718 | 0.806312 | 8.74E-50 | -1.69E-46 |
| MS.gene057448 | MS.gene92718 | 0.823495 | 1.34E-53 | -1.69E-46 |
| MS.gene057449 | MS.gene92718 | 0.815062 | 1.12E-51 | -1.69E-46 |
| MS.gene057450 | MS.gene92718 | 0.840565 | 8.05E-58 | -1.69E-46 |
| MS.gene058002 | MS.gene92718 | 0.814133 | 1.80E-51 | -1.69E-46 |
| MS.gene058006 | MS.gene92718 | 0.805277 | 1.44E-49 | -1.69E-46 |
| MS.gene058082 | MS.gene92718 | 0.81566 | 8.25E-52 | -1.69E-46 |
| MS.gene05811 | MS.gene92718 | 0.817596 | 3.04E-52 | -1.69E-46 |
| MS.gene058233 | MS.gene92718 | 0.80453 | 2.07E-49 | -1.69E-46 |
| MS.gene058293 | MS.gene92718 | 0.880882 | 3.81E-70 | -1.69E-46 |
| MS.gene058323 | MS.gene92718 | 0.817711 | 2.86E-52 | -1.69E-46 |
| MS.gene058365 | MS.gene92718 | 0.824672 | 7.11E-54 | -1.69E-46 |
| MS.gene058660 | MS.gene92718 | 0.833324 | 5.70E-56 | -1.69E-46 |
| MS.gene058787 | MS.gene92718 | 0.805918 | 1.06E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene92718 | 0.815225 | 1.03E-51 | -1.69E-46 |
| MS.gene059228 | MS.gene92718 | 0.806731 | 7.13E-50 | -1.69E-46 |
| MS.gene059326 | MS.gene92718 | 0.849639 | 2.84E-60 | -1.69E-46 |
| MS.gene059366 | MS.gene92718 | 0.822355 | 2.48E-53 | -1.69E-46 |
| MS.gene059518 | MS.gene92718 | 0.821746 | 3.43E-53 | -1.69E-46 |
| MS.gene059597 | MS.gene92718 | 0.806018 | 1.01E-49 | -1.69E-46 |
| MS.gene05959 | MS.gene92718 | 0.806238 | 9.07E-50 | -1.69E-46 |
| MS.gene059676 | MS.gene92718 | 0.857087 | 2.07E-62 | -1.69E-46 |
| MS.gene059688 | MS.gene92718 | 0.863031 | 3.33E-64 | -1.69E-46 |
| MS.gene059692 | MS.gene92718 | 0.882243 | 1.23E-70 | -1.69E-46 |
| MS.gene059695 | MS.gene92718 | 0.87929 | 1.41E-69 | -1.69E-46 |
| MS.gene059711 | MS.gene92718 | 0.829882 | 4.01E-55 | -1.69E-46 |
| MS.gene059894 | MS.gene92718 | 0.858847 | 6.22E-63 | -1.69E-46 |
| MS.gene059960 | MS.gene92718 | 0.849655 | 2.81E-60 | -1.69E-46 |
| MS.gene059974 | MS.gene92718 | 0.877145 | 7.95E-69 | -1.69E-46 |
| MS.gene060005 | MS.gene92718 | 0.840693 | 7.46E-58 | -1.69E-46 |
| MS.gene06037 | MS.gene92718 | 0.853623 | 2.11E-61 | -1.69E-46 |
| MS.gene060804 | MS.gene92718 | 0.815525 | 8.84E-52 | -1.69E-46 |
| MS.gene060818 | MS.gene92718 | 0.819069 | 1.41E-52 | -1.69E-46 |
| MS.gene060837 | MS.gene92718 | 0.80546 | 1.32E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene92718 | 0.850805 | 1.34E-60 | -1.69E-46 |
| MS.gene061051 | MS.gene92718 | -0.852307 | 5.03E-61 | -1.69E-46 |
| MS.gene061154 | MS.gene92718 | 0.80953 | 1.81E-50 | -1.69E-46 |
| MS.gene061200 | MS.gene92718 | 0.831256 | 1.85E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92718.t1 | MTR_3g030850 | 89.623 | 106 | 10 | 1 | 1 | 105 | 1 | 106 | 1.15e-60 | 181 |
| MS.gene92718.t1 | MTR_3g030860 | 86.538 | 104 | 14 | 0 | 1 | 104 | 1 | 104 | 3.52e-52 | 159 |
| MS.gene92718.t1 | MTR_4g037505 | 70.093 | 107 | 29 | 3 | 1 | 104 | 1 | 107 | 3.03e-42 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92718.t1 | AT1G51400 | 59.259 | 108 | 39 | 3 | 1 | 105 | 1 | 106 | 3.91e-31 | 106 |
| MS.gene92718.t1 | AT3G21055 | 58.491 | 106 | 38 | 4 | 1 | 104 | 1 | 102 | 6.68e-26 | 93.2 |
| MS.gene92718.t1 | AT3G21055 | 58.491 | 106 | 38 | 4 | 1 | 104 | 41 | 142 | 1.17e-25 | 93.6 |
Find 27 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGCTGTTTGCTCTGTTGC+TGG | 0.185357 | 3.1:-22830937 | MS.gene92718:CDS |
| TCCATTGCTGCCTTCTTTGT+TGG | 0.251840 | 3.1:+22830990 | None:intergenic |
| CTGCTGTTTGCTCTGTTGCT+GGG | 0.300217 | 3.1:-22830936 | MS.gene92718:CDS |
| ATCCTCAATCCTTGGCTTCC+CGG | 0.339335 | 3.1:-22831106 | MS.gene92718:CDS |
| CTCTGTTGCTGGGGTTGCAA+TGG | 0.391387 | 3.1:-22830926 | MS.gene92718:CDS |
| AATGCTGTCCGAGCAGTTGA+TGG | 0.443602 | 3.1:-22831036 | MS.gene92718:CDS |
| GCTTCCCGGCTGTAACCAAC+CGG | 0.484721 | 3.1:-22831092 | MS.gene92718:CDS |
| GTTGGCATAGTGACACAAAC+AGG | 0.491066 | 3.1:+22830853 | None:intergenic |
| TGCTGTTTGCTCTGTTGCTG+GGG | 0.508878 | 3.1:-22830935 | MS.gene92718:CDS |
| ACCATGACATCCTCAATCCT+TGG | 0.515033 | 3.1:-22831114 | MS.gene92718:CDS |
| ATGCTGTCCGAGCAGTTGAT+GGG | 0.515597 | 3.1:-22831035 | MS.gene92718:CDS |
| ACCAACAAAGAAGGCAGCAA+TGG | 0.518827 | 3.1:-22830991 | MS.gene92718:CDS |
| AGCCGGGAAGCCAAGGATTG+AGG | 0.543329 | 3.1:+22831104 | None:intergenic |
| TCTGCGATGCTACGGATGAC+CGG | 0.544879 | 3.1:+22831073 | None:intergenic |
| ACAAAGAAGGCAGCAATGGA+AGG | 0.546411 | 3.1:-22830987 | MS.gene92718:CDS |
| AAATATGAAACCAACAAAGA+AGG | 0.547747 | 3.1:-22831000 | MS.gene92718:CDS |
| GAGTCTCCTCTGCGATGCTA+CGG | 0.551751 | 3.1:+22831065 | None:intergenic |
| TGGTTACAGCCGGGAAGCCA+AGG | 0.559386 | 3.1:+22831097 | None:intergenic |
| GATGACCGGTTGGTTACAGC+CGG | 0.561463 | 3.1:+22831087 | None:intergenic |
| GCCAAGGATTGAGGATGTCA+TGG | 0.568229 | 3.1:+22831113 | None:intergenic |
| GTGTCACTATGCCAACAGCT+AGG | 0.576409 | 3.1:-22830846 | MS.gene92718:CDS |
| TTGCGACAAATCCTAGCTGT+TGG | 0.581063 | 3.1:+22830835 | None:intergenic |
| CGATGCTACGGATGACCGGT+TGG | 0.597909 | 3.1:+22831077 | None:intergenic |
| TGCTGTCCGAGCAGTTGATG+GGG | 0.601918 | 3.1:-22831034 | MS.gene92718:CDS |
| GCAGATGATGAACCTAAAAG+AGG | 0.606913 | 3.1:-22830904 | MS.gene92718:CDS |
| ATGACCGGTTGGTTACAGCC+GGG | 0.647755 | 3.1:+22831088 | None:intergenic |
| GGTCATCCGTAGCATCGCAG+AGG | 0.728401 | 3.1:-22831071 | MS.gene92718:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATATGAAACCAACAAAGA+AGG | - | chr3.1:22830957-22830976 | MS.gene92718:CDS | 25.0% |
| !!! | ATACTTTTTCTTTGCTTCTG+GGG | + | chr3.1:22831081-22831100 | None:intergenic | 30.0% |
| !!! | CATACTTTTTCTTTGCTTCT+GGG | + | chr3.1:22831082-22831101 | None:intergenic | 30.0% |
| !!! | GCATACTTTTTCTTTGCTTC+TGG | + | chr3.1:22831083-22831102 | None:intergenic | 35.0% |
| GCAGATGATGAACCTAAAAG+AGG | - | chr3.1:22831053-22831072 | MS.gene92718:CDS | 40.0% | |
| ACAAAGAAGGCAGCAATGGA+AGG | - | chr3.1:22830970-22830989 | MS.gene92718:CDS | 45.0% | |
| ACCAACAAAGAAGGCAGCAA+TGG | - | chr3.1:22830966-22830985 | MS.gene92718:CDS | 45.0% | |
| ACCATGACATCCTCAATCCT+TGG | - | chr3.1:22830843-22830862 | MS.gene92718:CDS | 45.0% | |
| GTTGGCATAGTGACACAAAC+AGG | + | chr3.1:22831107-22831126 | None:intergenic | 45.0% | |
| TCCATTGCTGCCTTCTTTGT+TGG | + | chr3.1:22830970-22830989 | None:intergenic | 45.0% | |
| TTGCGACAAATCCTAGCTGT+TGG | + | chr3.1:22831125-22831144 | None:intergenic | 45.0% | |
| ! | TCTTTTCCCCATCAACTGCT+CGG | + | chr3.1:22830932-22830951 | None:intergenic | 45.0% |
| AATGCTGTCCGAGCAGTTGA+TGG | - | chr3.1:22830921-22830940 | MS.gene92718:CDS | 50.0% | |
| ATCCTCAATCCTTGGCTTCC+CGG | - | chr3.1:22830851-22830870 | MS.gene92718:CDS | 50.0% | |
| ATGCTGTCCGAGCAGTTGAT+GGG | - | chr3.1:22830922-22830941 | MS.gene92718:CDS | 50.0% | |
| GCCAAGGATTGAGGATGTCA+TGG | + | chr3.1:22830847-22830866 | None:intergenic | 50.0% | |
| ! | CTGCTGTTTGCTCTGTTGCT+GGG | - | chr3.1:22831021-22831040 | MS.gene92718:CDS | 50.0% |
| ! | GTGTCACTATGCCAACAGCT+AGG | - | chr3.1:22831111-22831130 | MS.gene92718:CDS | 50.0% |
| ! | TGCTGTTTGCTCTGTTGCTG+GGG | - | chr3.1:22831022-22831041 | MS.gene92718:CDS | 50.0% |
| !!! | GCTTCTGGGGTACCTCTTTT+AGG | + | chr3.1:22831068-22831087 | None:intergenic | 50.0% |
| CTCTGTTGCTGGGGTTGCAA+TGG | - | chr3.1:22831031-22831050 | MS.gene92718:CDS | 55.0% | |
| GAGTCTCCTCTGCGATGCTA+CGG | + | chr3.1:22830895-22830914 | None:intergenic | 55.0% | |
| TCTGCGATGCTACGGATGAC+CGG | + | chr3.1:22830887-22830906 | None:intergenic | 55.0% | |
| ! | ATGACCGGTTGGTTACAGCC+GGG | + | chr3.1:22830872-22830891 | None:intergenic | 55.0% |
| ! | GATGACCGGTTGGTTACAGC+CGG | + | chr3.1:22830873-22830892 | None:intergenic | 55.0% |
| ! | GCTGCTGTTTGCTCTGTTGC+TGG | - | chr3.1:22831020-22831039 | MS.gene92718:CDS | 55.0% |
| ! | TGCTGTCCGAGCAGTTGATG+GGG | - | chr3.1:22830923-22830942 | MS.gene92718:CDS | 55.0% |
| AGCCGGGAAGCCAAGGATTG+AGG | + | chr3.1:22830856-22830875 | None:intergenic | 60.0% | |
| CGATGCTACGGATGACCGGT+TGG | + | chr3.1:22830883-22830902 | None:intergenic | 60.0% | |
| GCTTCCCGGCTGTAACCAAC+CGG | - | chr3.1:22830865-22830884 | MS.gene92718:CDS | 60.0% | |
| GGTCATCCGTAGCATCGCAG+AGG | - | chr3.1:22830886-22830905 | MS.gene92718:CDS | 60.0% | |
| TGGTTACAGCCGGGAAGCCA+AGG | + | chr3.1:22830863-22830882 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 22830831 | 22831148 | 22830831 | ID=MS.gene92718 |
| chr3.1 | mRNA | 22830831 | 22831148 | 22830831 | ID=MS.gene92718.t1;Parent=MS.gene92718 |
| chr3.1 | exon | 22830831 | 22831148 | 22830831 | ID=MS.gene92718.t1.exon1;Parent=MS.gene92718.t1 |
| chr3.1 | CDS | 22830831 | 22831148 | 22830831 | ID=cds.MS.gene92718.t1;Parent=MS.gene92718.t1 |
| Gene Sequence |
| Protein sequence |
