AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96838


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96838.t1 MTR_7g062470 91.603 131 6 1 1 126 1 131 4.82e-70 206
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96838.t1 AT5G02160 84.810 79 12 0 48 126 50 128 3.23e-46 146

Find 28 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAACACTAAACTCTTATT+TGG 0.131404 7.3:+42659719 MS.gene96838:CDS
TATCTTGTTGCTGGTGCTAT+TGG 0.295597 7.3:+42659839 MS.gene96838:CDS
GGCACTATTTATCTTGTTGC+TGG 0.299445 7.3:+42659830 MS.gene96838:CDS
GTGATGGAGCAGGGTTTGTT+AGG 0.314720 7.3:+42661193 MS.gene96838:CDS
AAATAAGAGTTTAGTGTTGT+TGG 0.324277 7.3:-42659717 None:intergenic
GCTATTGGAATTGCTCTTGT+TGG 0.368974 7.3:+42659854 MS.gene96838:CDS
TTGTGCTCGTTGTGATGGTT+TGG 0.405035 7.3:+42661272 MS.gene96838:CDS
AGCATTTCTATTTGTCAACT+TGG 0.409660 7.3:-42661305 None:intergenic
AACCCTGCTCCATCACAATC+AGG 0.417979 7.3:-42661186 None:intergenic
ATTGGGAATGATTGTGTTCA+AGG 0.420585 7.3:-42659684 None:intergenic
TCAGTTGTTGTTTCAGCAGT+TGG 0.426749 7.3:+42659791 MS.gene96838:CDS
TGTGCTCGTTGTGATGGTTT+GGG 0.434742 7.3:+42661273 MS.gene96838:CDS
AACACTAAACTCTTATTTGG+AGG 0.444395 7.3:+42659722 MS.gene96838:CDS
TCTTTACGTGAGAAGATGAT+AGG 0.453999 7.3:-42659887 None:intergenic
CAGGGTTTGTTAGGAAAGGT+GGG 0.459410 7.3:+42661202 MS.gene96838:CDS
GCAGGGTTTGTTAGGAAAGG+TGG 0.460825 7.3:+42661201 MS.gene96838:CDS
GAGAGCAAATGCAGCAAGAA+AGG 0.478276 7.3:+42661233 MS.gene96838:CDS
GTTGTTGGAAATTGTAACAT+TGG 0.483393 7.3:-42659702 None:intergenic
TGCCCTGATTGTGATGGAGC+AGG 0.517909 7.3:+42661183 MS.gene96838:CDS
TTGTTGGAAATTGTAACATT+GGG 0.522805 7.3:-42659701 None:intergenic
ATTGTTTGTGCTCGTTGTGA+TGG 0.530028 7.3:+42661267 MS.gene96838:CDS
GGAGCAGGGTTTGTTAGGAA+AGG 0.545979 7.3:+42661198 MS.gene96838:CDS
AATGATTGTGTTCAAGGTCA+TGG 0.560862 7.3:-42659678 None:intergenic
GTTGGAGATGTATCTTCTGA+TGG 0.593310 7.3:+42659809 MS.gene96838:CDS
ACCCTGCTCCATCACAATCA+GGG 0.608104 7.3:-42661185 None:intergenic
GCCCTGATTGTGATGGAGCA+GGG 0.608764 7.3:+42661184 MS.gene96838:CDS
ATCAGATGCCCTGATTGTGA+TGG 0.647435 7.3:+42661177 MS.gene96838:intron
AGGGTTTGTTAGGAAAGGTG+GGG 0.670666 7.3:+42661203 MS.gene96838:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTATAAATATTTATAT+TGG - chr7.3:42660547-42660566 None:intergenic 0.0%
!! AAATTATAAATATTTATATT+GGG - chr7.3:42660546-42660565 None:intergenic 0.0%
!! TAAAAAATAAAATCTGATTT+CGG + chr7.3:42660370-42660389 MS.gene96838:intron 10.0%
!! AATAATAATCTCAATAATAG+TGG + chr7.3:42660571-42660590 MS.gene96838:intron 15.0%
!!! TAATTATAAACCTATTTTTG+TGG + chr7.3:42660974-42660993 MS.gene96838:intron 15.0%
!! AATCTTAAAACCACAAAAAT+AGG - chr7.3:42660987-42661006 None:intergenic 20.0%
!! GATAATGCATAATATATGTA+AGG + chr7.3:42660465-42660484 MS.gene96838:intron 20.0%
!!! AAATTTTGTATAGGCATTTT+TGG + chr7.3:42660101-42660120 MS.gene96838:intron 20.0%
!!! TTCTAAGAGAAATTTTGTAT+AGG + chr7.3:42660092-42660111 MS.gene96838:intron 20.0%
!!! TTTTTTCATTGATTCTTGTT+TGG + chr7.3:42661076-42661095 MS.gene96838:intron 20.0%
! AAATAAGAGTTTAGTGTTGT+TGG - chr7.3:42659720-42659739 None:intergenic 25.0%
! AACAACACTAAACTCTTATT+TGG + chr7.3:42659719-42659738 MS.gene96838:CDS 25.0%
! ACATCATTAACAAACAACAA+AGG - chr7.3:42661140-42661159 None:intergenic 25.0%
! GGTAAAACTATACTTATCTA+TGG - chr7.3:42660304-42660323 None:intergenic 25.0%
! TGCACTTGAAATAAAAATCT+AGG - chr7.3:42661052-42661071 None:intergenic 25.0%
! TTGTTGGAAATTGTAACATT+GGG - chr7.3:42659704-42659723 None:intergenic 25.0%
!! ATTCTGAGAAAATTAGAGAT+AGG - chr7.3:42660188-42660207 None:intergenic 25.0%
!!! AAGAGAATTGTTTTTTTTCG+TGG - chr7.3:42660513-42660532 None:intergenic 25.0%
!!! ACCTAGTTTTTTAATCTACT+TGG + chr7.3:42661015-42661034 MS.gene96838:intron 25.0%
!!! CATCGTTATTTTCATTTTCA+TGG + chr7.3:42659924-42659943 MS.gene96838:intron 25.0%
!!! CCTAGTTTTTTAATCTACTT+GGG + chr7.3:42661016-42661035 MS.gene96838:intron 25.0%
!!! TAAGTATAGTTTTACCTTGA+CGG + chr7.3:42660308-42660327 MS.gene96838:intron 25.0%
CCCAAGTAGATTAAAAAACT+AGG - chr7.3:42661019-42661038 None:intergenic 30.0%
GTTGTTGGAAATTGTAACAT+TGG - chr7.3:42659705-42659724 None:intergenic 30.0%
TCAATAAAAAACATCCGTCA+AGG - chr7.3:42660325-42660344 None:intergenic 30.0%
TCAATAATAGTGGTTGAAAC+TGG + chr7.3:42660581-42660600 MS.gene96838:intron 30.0%
TGAACAAATTACACTTGCAA+TGG + chr7.3:42660034-42660053 MS.gene96838:intron 30.0%
TGATAATTGGCAATGATGAT+TGG + chr7.3:42660674-42660693 MS.gene96838:intron 30.0%
TGCATAATATATGTAAGGTC+CGG + chr7.3:42660470-42660489 MS.gene96838:intron 30.0%
! AACACTAAACTCTTATTTGG+AGG + chr7.3:42659722-42659741 MS.gene96838:CDS 30.0%
!! TCATTGATTCTTGTTTGGAA+TGG + chr7.3:42661081-42661100 MS.gene96838:intron 30.0%
!!! GTGGTCATAGTAGTTTTAAA+TGG + chr7.3:42660766-42660785 MS.gene96838:intron 30.0%
AATGATTGTGTTCAAGGTCA+TGG - chr7.3:42659681-42659700 None:intergenic 35.0%
ACAATGATCGGAGTGATAAT+TGG + chr7.3:42660661-42660680 MS.gene96838:intron 35.0%
ATCTAAGAAAGTTGGAGTGA+TGG + chr7.3:42660852-42660871 MS.gene96838:intron 35.0%
ATTGGGAATGATTGTGTTCA+AGG - chr7.3:42659687-42659706 None:intergenic 35.0%
CATAATATATGTAAGGTCCG+GGG + chr7.3:42660472-42660491 MS.gene96838:intron 35.0%
GCATAATATATGTAAGGTCC+GGG + chr7.3:42660471-42660490 MS.gene96838:intron 35.0%
TAATTGGCAATGATGATTGG+CGG + chr7.3:42660677-42660696 MS.gene96838:intron 35.0%
TAGAGGTGATCTAAGAAAGT+TGG + chr7.3:42660844-42660863 MS.gene96838:intron 35.0%
TCTTTACGTGAGAAGATGAT+AGG - chr7.3:42659890-42659909 None:intergenic 35.0%
TTGAACTTTGCTCAGTTGAT+AGG + chr7.3:42660442-42660461 MS.gene96838:intron 35.0%
! TGAACTTTGCTCAGTTGATA+GGG + chr7.3:42660443-42660462 MS.gene96838:intron 35.0%
! TTGAAACTGGTTGTTGTTAG+AGG + chr7.3:42660594-42660613 MS.gene96838:intron 35.0%
!! GTTTTAAATGGTGATCACAG+TGG + chr7.3:42660778-42660797 MS.gene96838:intron 35.0%
AACGTTTCATGTTACCACCA+AGG - chr7.3:42660941-42660960 None:intergenic 40.0%
ACGATCAGAATGATGATTGG+TGG + chr7.3:42660713-42660732 MS.gene96838:intron 40.0%
AGTGGTGATCAGAGTATATG+TGG + chr7.3:42660796-42660815 MS.gene96838:intron 40.0%
ATTGTTTGTGCTCGTTGTGA+TGG + chr7.3:42661267-42661286 MS.gene96838:CDS 40.0%
CAAACAGTGGCTACAATGAT+CGG + chr7.3:42660649-42660668 MS.gene96838:intron 40.0%
GCAACGATCAGAATGATGAT+TGG + chr7.3:42660710-42660729 MS.gene96838:intron 40.0%
GCTATTGGAATTGCTCTTGT+TGG + chr7.3:42659854-42659873 MS.gene96838:CDS 40.0%
GGCACTATTTATCTTGTTGC+TGG + chr7.3:42659830-42659849 MS.gene96838:CDS 40.0%
GTTGGAGATGTATCTTCTGA+TGG + chr7.3:42659809-42659828 MS.gene96838:CDS 40.0%
! AAACTGGTTGTTGTTAGAGG+AGG + chr7.3:42660597-42660616 MS.gene96838:intron 40.0%
! AGAATGATGATTGGTGGACA+AGG + chr7.3:42660719-42660738 MS.gene96838:intron 40.0%
! GAATGATGATTGGTGGACAA+GGG + chr7.3:42660720-42660739 MS.gene96838:intron 40.0%
!! GAGTATATGTGGTGTCATAG+TGG + chr7.3:42660807-42660826 MS.gene96838:intron 40.0%
!! TATATGTGGTGTCATAGTGG+TGG + chr7.3:42660810-42660829 MS.gene96838:intron 40.0%
!! TATCTTGTTGCTGGTGCTAT+TGG + chr7.3:42659839-42659858 MS.gene96838:CDS 40.0%
!! TCAGTTGTTGTTTCAGCAGT+TGG + chr7.3:42659791-42659810 MS.gene96838:CDS 40.0%
!!! GTTTTTTTTCGTGGTAGCCA+AGG - chr7.3:42660504-42660523 None:intergenic 40.0%
AGTTGGAGTGATGGTTGACT+TGG + chr7.3:42660861-42660880 MS.gene96838:intron 45.0%
ATCAGATGCCCTGATTGTGA+TGG + chr7.3:42661177-42661196 MS.gene96838:intron 45.0%
GAGAGCAAATGCAGCAAGAA+AGG + chr7.3:42661233-42661252 MS.gene96838:CDS 45.0%
GAGTGGTGAATAGCAAACAG+TGG + chr7.3:42660636-42660655 MS.gene96838:intron 45.0%
TGTGCTCGTTGTGATGGTTT+GGG + chr7.3:42661273-42661292 MS.gene96838:CDS 45.0%
TTGTGCTCGTTGTGATGGTT+TGG + chr7.3:42661272-42661291 MS.gene96838:CDS 45.0%
! CTATGACCACTAATCGACTC+CGG - chr7.3:42660756-42660775 None:intergenic 45.0%
! GTTGTTGTTAGAGGAGGTGT+TGG + chr7.3:42660603-42660622 MS.gene96838:intron 45.0%
!! AGGAGGTGTTGGACAACTAT+CGG + chr7.3:42660614-42660633 MS.gene96838:intron 45.0%
!! AGGGTTTGTTAGGAAAGGTG+GGG + chr7.3:42661203-42661222 MS.gene96838:CDS 45.0%
!! CAGGGTTTGTTAGGAAAGGT+GGG + chr7.3:42661202-42661221 MS.gene96838:CDS 45.0%
!! GTCTTAGTGTTGGTCGTTGA+TGG + chr7.3:42660907-42660926 MS.gene96838:intron 45.0%
AACCCTGCTCCATCACAATC+AGG - chr7.3:42661189-42661208 None:intergenic 50.0%
ACCCTGCTCCATCACAATCA+GGG - chr7.3:42661188-42661207 None:intergenic 50.0%
GTGTTGGACAACTATCGGAG+TGG + chr7.3:42660619-42660638 MS.gene96838:intron 50.0%
TGATGGTCATCGATCCTTGG+TGG + chr7.3:42660924-42660943 MS.gene96838:intron 50.0%
! AATGATGATTGGCGGACAGG+TGG + chr7.3:42660685-42660704 MS.gene96838:intron 50.0%
! GTGATGGAGCAGGGTTTGTT+AGG + chr7.3:42661193-42661212 MS.gene96838:CDS 50.0%
! TGGTGGACAAGGGATTGCAA+TGG + chr7.3:42660730-42660749 MS.gene96838:intron 50.0%
!! CGTTGATGGTCATCGATCCT+TGG + chr7.3:42660921-42660940 MS.gene96838:intron 50.0%
!! GCAGGGTTTGTTAGGAAAGG+TGG + chr7.3:42661201-42661220 MS.gene96838:CDS 50.0%
!! GGAGCAGGGTTTGTTAGGAA+AGG + chr7.3:42661198-42661217 MS.gene96838:CDS 50.0%
!! GGAGTGATGGTTGACTTGGT+CGG + chr7.3:42660865-42660884 MS.gene96838:intron 50.0%
!! GTTGGTGGTGGTCTTAGTGT+TGG + chr7.3:42660897-42660916 MS.gene96838:intron 50.0%
!! TGTGGTGTCATAGTGGTGGT+TGG + chr7.3:42660814-42660833 MS.gene96838:intron 50.0%
AAGGTCCGGGGTTCAAACCT+TGG + chr7.3:42660484-42660503 MS.gene96838:intron 55.0%
CAATGGCCGGAGTCGATTAG+TGG + chr7.3:42660747-42660766 MS.gene96838:intron 55.0%
GGACAAGGGATTGCAATGGC+CGG + chr7.3:42660734-42660753 MS.gene96838:intron 55.0%
GGTAGCCAAGGTTTGAACCC+CGG - chr7.3:42660492-42660511 None:intergenic 55.0%
! GCCCTGATTGTGATGGAGCA+GGG + chr7.3:42661184-42661203 MS.gene96838:CDS 55.0%
! GGCAATGATGATTGGCGGAC+AGG + chr7.3:42660682-42660701 MS.gene96838:intron 55.0%
! GGTGTCATAGTGGTGGTTGG+TGG + chr7.3:42660817-42660836 MS.gene96838:intron 55.0%
! TGCCCTGATTGTGATGGAGC+AGG + chr7.3:42661183-42661202 MS.gene96838:CDS 55.0%
!! GTCATAGTGGTGGTTGGTGG+TGG + chr7.3:42660820-42660839 MS.gene96838:intron 55.0%
!! GTGATGGTTGACTTGGTCGG+TGG + chr7.3:42660868-42660887 MS.gene96838:intron 55.0%
!! TGGTGGTTGGTGGTGGTTAG+AGG + chr7.3:42660827-42660846 MS.gene96838:intron 55.0%
!! TTGACTTGGTCGGTGGTGAG+TGG + chr7.3:42660875-42660894 MS.gene96838:intron 55.0%
CTTGGTCGGTGGTGAGTGGT+TGG + chr7.3:42660879-42660898 MS.gene96838:intron 60.0%
GGTCGGTGGTGAGTGGTTGG+TGG + chr7.3:42660882-42660901 MS.gene96838:intron 65.0%
!! CGGTGGTGAGTGGTTGGTGG+TGG + chr7.3:42660885-42660904 MS.gene96838:intron 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 42659671 42661320 42659671 ID=MS.gene96838
chr7.3 mRNA 42659671 42661320 42659671 ID=MS.gene96838.t1;Parent=MS.gene96838
chr7.3 exon 42659671 42659912 42659671 ID=MS.gene96838.t1.exon1;Parent=MS.gene96838.t1
chr7.3 CDS 42659671 42659912 42659671 ID=cds.MS.gene96838.t1;Parent=MS.gene96838.t1
chr7.3 exon 42661182 42661320 42661182 ID=MS.gene96838.t1.exon2;Parent=MS.gene96838.t1
chr7.3 CDS 42661182 42661320 42661182 ID=cds.MS.gene96838.t1;Parent=MS.gene96838.t1
Gene Sequence

>MS.gene96838

ATGGCATCCATGACCTTGAACACAATCATTCCCAATGTTACAATTTCCAACAACACTAAACTCTTATTTGGAGGCTCAACAAATGTGTCATGTAACAAGATTCATCAGAAGAGATCTTCATCAGTTGTTGTTTCAGCAGTTGGAGATGTATCTTCTGATGGCACTATTTATCTTGTTGCTGGTGCTATTGGAATTGCTCTTGTTGGAACTGCATTTCCTATCATCTTCTCACGTAAAGACACGTAAGTAACATCATCGTTATTTTCATTTTCATGGTTTTATATTTTCATTTCAATTATAGTTTTACATGTTTCATGATCTTTATTTTGTTAATCTTTTGTTATATTATGCTTTTTGATAATGTGAACAAATTACACTTGCAATGGAAACGACTATAATTTGAAGACTTAACATTTTTTTATTCTAAGAGAAATTTTGTATAGGCATTTTTGGATAGTTATTTTAATAAAGAAAAATGAATTACAACTAGAAATTAGAGATTGAAAAAATGAAACCTATCTCTAATTTTCTCAGAATTGTTAGAGATGTAAAAATCAGAAATTAATTTCTTATATTTTGAGTCTAAGTTATATACACAAAAATCATTAATTAACATTTGTGAAGATATTTCCATAGATAAGTATAGTTTTACCTTGACGGATGTTTTTTATTGATTTTAAGTTTTTATTTTGAAATTTTTAAAAAATAAAATCTGATTTCGGCTTTTAGTTTCGAATTTCGACTCTAAGTTGAGTTTAAAGTTTTTTTTAATTGAACTTTGCTCAGTTGATAGGGATAATGCATAATATATGTAAGGTCCGGGGTTCAAACCTTGGCTACCACGAAAAAAAACAATTCTCTTTATGAATTTACCCAATATAAATATTTATAATTTTATTTAATAATAATCTCAATAATAGTGGTTGAAACTGGTTGTTGTTAGAGGAGGTGTTGGACAACTATCGGAGTGGTGAATAGCAAACAGTGGCTACAATGATCGGAGTGATAATTGGCAATGATGATTGGCGGACAGGTGGTTGCAACGATCAGAATGATGATTGGTGGACAAGGGATTGCAATGGCCGGAGTCGATTAGTGGTCATAGTAGTTTTAAATGGTGATCACAGTGGTGATCAGAGTATATGTGGTGTCATAGTGGTGGTTGGTGGTGGTTAGAGGTGATCTAAGAAAGTTGGAGTGATGGTTGACTTGGTCGGTGGTGAGTGGTTGGTGGTGGTCTTAGTGTTGGTCGTTGATGGTCATCGATCCTTGGTGGTAACATGAAACGTTAAAATAAATCAAATAATTATAAACCTATTTTTGTGGTTTTAAGATTTAAGTGAAACCTAGTTTTTTAATCTACTTGGGAATATTTTCTCCTAGATTTTTATTTCAAGTGCATGTTTTTTTTCATTGATTCTTGTTTGGAATGGTTTAAACATGTAAAAAAAAGAACGTAACTATGTCCTTTGTTGTTTGTTAATGATGTTATTGTTGAAACATTGTATCAGATGCCCTGATTGTGATGGAGCAGGGTTTGTTAGGAAAGGTGGGGTGACATTGAGAGCAAATGCAGCAAGAAAGGATCAAACTCAAATTGTTTGTGCTCGTTGTGATGGTTTGGGAAAACTTAACCAAGTTGACAAATAG

Protein sequence

>MS.gene96838.t1

MASMTLNTIIPNVTISNNTKLLFGGSTNVSCNKIHQKRSSSVVVSAVGDVSSDGTIYLVAGAIGIALVGTAFPIIFSRKDTCPDCDGAGFVRKGGVTLRANAARKDQTQIVCARCDGLGKLNQVDK