Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030303.01.T01 | XP_024641352.1 | 62.069 | 261 | 77 | 10 | 1 | 245 | 1 | 255 | 7.01E-87 | 273 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000047.01 | MsG0680030303.01 | 0.844158 | 8.987499e-59 | 1.438315e-55 |
| MsG0180000271.01 | MsG0680030303.01 | 0.812096 | 5.034212e-51 | 3.171401e-48 |
| MsG0180000526.01 | MsG0680030303.01 | 0.857556 | 1.505871e-62 | 3.731512e-59 |
| MsG0180000550.01 | MsG0680030303.01 | 0.814959 | 1.180998e-51 | 8.036514e-49 |
| MsG0180000653.01 | MsG0680030303.01 | 0.804701 | 1.903686e-49 | 9.882314e-47 |
| MsG0180000673.01 | MsG0680030303.01 | 0.805365 | 1.382578e-49 | 7.300968e-47 |
| MsG0180000797.01 | MsG0680030303.01 | 0.821506 | 3.894669e-53 | 3.174342e-50 |
| MsG0180000822.01 | MsG0680030303.01 | 0.806661 | 7.379713e-50 | 4.028241e-47 |
| MsG0180000935.01 | MsG0680030303.01 | 0.802742 | 4.854990e-49 | 2.396794e-46 |
| MsG0180001355.01 | MsG0680030303.01 | 0.812534 | 4.040565e-51 | 2.575628e-48 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030303.01.T01 | MTR_6g018315 | 65.702 | 242 | 66 | 9 | 1 | 231 | 128 | 363 | 2.38e-89 | 273 |
| MsG0680030303.01.T01 | MTR_6g018360 | 65.702 | 242 | 66 | 10 | 1 | 231 | 143 | 378 | 2.60e-89 | 274 |
| MsG0680030303.01.T01 | MTR_2g047180 | 59.641 | 223 | 74 | 6 | 13 | 231 | 153 | 363 | 1.15e-81 | 253 |
| MsG0680030303.01.T01 | MTR_6g027100 | 61.472 | 231 | 73 | 7 | 14 | 242 | 168 | 384 | 5.85e-81 | 253 |
| MsG0680030303.01.T01 | MTR_6g027040 | 63.761 | 218 | 63 | 7 | 14 | 229 | 168 | 371 | 1.60e-80 | 250 |
| MsG0680030303.01.T01 | MTR_6g018390 | 63.801 | 221 | 64 | 7 | 11 | 230 | 157 | 362 | 2.60e-79 | 246 |
| MsG0680030303.01.T01 | MTR_3g029650 | 60.829 | 217 | 75 | 6 | 18 | 231 | 149 | 358 | 7.53e-77 | 250 |
| MsG0680030303.01.T01 | MTR_3g029650 | 47.111 | 225 | 101 | 8 | 14 | 229 | 531 | 746 | 4.25e-46 | 167 |
| MsG0680030303.01.T01 | MTR_6g018400 | 58.874 | 231 | 80 | 7 | 1 | 230 | 149 | 365 | 9.18e-75 | 235 |
| MsG0680030303.01.T01 | MTR_1g026850 | 57.269 | 227 | 71 | 8 | 15 | 230 | 149 | 360 | 1.19e-68 | 219 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030303.01.T01 | AT3G18280 | 49.438 | 89 | 42 | 1 | 246 | 334 | 35 | 120 | 1.38e-28 | 107 |
| MsG0680030303.01.T01 | AT3G18280 | 49.438 | 89 | 42 | 1 | 246 | 334 | 11 | 96 | 8.51e-28 | 104 |
| MsG0680030303.01.T01 | AT2G17036 | 37.056 | 197 | 103 | 6 | 50 | 230 | 189 | 380 | 1.08e-27 | 112 |
| MsG0680030303.01.T01 | AT2G17036 | 37.056 | 197 | 103 | 6 | 50 | 230 | 190 | 381 | 1.08e-27 | 112 |
| MsG0680030303.01.T01 | AT1G48750 | 55.556 | 72 | 32 | 0 | 263 | 334 | 23 | 94 | 1.58e-26 | 101 |
| MsG0680030303.01.T01 | AT2G17030 | 32.636 | 239 | 131 | 10 | 14 | 230 | 157 | 387 | 6.51e-23 | 99.0 |
| MsG0680030303.01.T01 | AT1G66850 | 50.725 | 69 | 34 | 0 | 266 | 334 | 34 | 102 | 2.13e-20 | 85.1 |
| MsG0680030303.01.T01 | AT1G73780 | 34.694 | 98 | 64 | 0 | 237 | 334 | 1 | 98 | 1.80e-19 | 82.4 |
| MsG0680030303.01.T01 | AT1G43666 | 46.154 | 91 | 43 | 3 | 244 | 334 | 11 | 95 | 6.93e-18 | 78.2 |
| MsG0680030303.01.T01 | AT5G38170 | 45.588 | 68 | 37 | 0 | 267 | 334 | 36 | 103 | 3.99e-17 | 76.3 |
Find 84 sgRNAs with CRISPR-Local
Find 344 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACAAGGACTGAACTCTAC+AGG | 0.466330 | 6:-301593 | None:intergenic |
| AAAGATAACACAATTTCCTT+TGG | 0.357321 | 6:-297659 | None:intergenic |
| AAATTCTTAGTGGAGAGCGA+GGG | 0.683320 | 6:+297445 | MsG0680030303.01.T01:CDS |
| AACAAGGACTGAACTCTACA+GGG | 0.629235 | 6:-301592 | None:intergenic |
| AAGCAGTATATTTACTCTCC+TGG | 0.517554 | 6:+301716 | MsG0680030303.01.T01:CDS |
| AAGGGTTACTAGCTCTTGTG+GGG | 0.597115 | 6:+301745 | MsG0680030303.01.T01:CDS |
| AAGTCAATCGACATGTCATC+CGG | 0.450912 | 6:-297286 | None:intergenic |
| AATGCAGAAATGATGAGAAT+TGG | 0.409857 | 6:+297254 | MsG0680030303.01.T01:CDS |
| AATTGTCTTACCAAATTTGT+CGG | 0.441386 | 6:-297353 | None:intergenic |
| AGAAGGGTTACTAGCTCTTG+TGG | 0.498825 | 6:+301743 | MsG0680030303.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAAAGTTGTGATCATTCA+AGG | + | Chr6:298261-298280 | MsG0680030303.01.T01:intron | 30.0% | |
| AATGCAGAAATGATGAGAAT+TGG | + | Chr6:297254-297273 | MsG0680030303.01.T01:CDS | 30.0% | |
| ACAATGATAGACTCACTATT+GGG | - | Chr6:301298-301317 | None:intergenic | 30.0% | |
| ACATCGAAATAACATTACCA+AGG | - | Chr6:298405-298424 | None:intergenic | 30.0% | |
| ATAGAGATGCAATTGTGTTA+TGG | + | Chr6:301075-301094 | MsG0680030303.01.T01:intron | 30.0% | |
| ATATAATTATTCTGTGGCAG+GGG | + | Chr6:300656-300675 | MsG0680030303.01.T01:intron | 30.0% | |
| ATCTCAACTAAGTTTACCTA+AGG | + | Chr6:298488-298507 | MsG0680030303.01.T01:intron | 30.0% | |
| ATCTGTTATCTGTAGCTATT+TGG | + | Chr6:299457-299476 | MsG0680030303.01.T01:intron | 30.0% | |
| ATTCCCTGGGAAATAAAATA+TGG | - | Chr6:300714-300733 | None:intergenic | 30.0% | |
| ATTGTGATATGCTGTAATCA+AGG | - | Chr6:300836-300855 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 297118 | 301787 | 297118 | ID=MsG0680030303.01;Name=MsG0680030303.01 |
| Chr6 | mRNA | 297118 | 301787 | 297118 | ID=MsG0680030303.01.T01;Parent=MsG0680030303.01;Name=MsG0680030303.01.T01;_AED=0.24;_eAED=0.24;_QI=0|0|0|1|0|0|2|0|334 |
| Chr6 | exon | 297118 | 297809 | 297118 | ID=MsG0680030303.01.T01:exon:2033;Parent=MsG0680030303.01.T01 |
| Chr6 | exon | 301475 | 301787 | 301475 | ID=MsG0680030303.01.T01:exon:2034;Parent=MsG0680030303.01.T01 |
| Chr6 | CDS | 297118 | 297809 | 297118 | ID=MsG0680030303.01.T01:cds;Parent=MsG0680030303.01.T01 |
| Chr6 | CDS | 301475 | 301787 | 301475 | ID=MsG0680030303.01.T01:cds;Parent=MsG0680030303.01.T01 |
| Gene Sequence |
>MsG0680030303.01.T01 ATGGACAACTACGACACCACATTCCAATATAATGTCAACTACATTGGTCCTTTTAAGGTCGTTGCTGTCACATGCCATGGAAGAAGACCTCTTATTGTCGGCACGTTGAACTGTGTCCCACAGCCGATATTGTTGAAATGCAGAAATGATGAGAATTGGAAGGTAATCCCGGATGACATGTCGATTGACTTTTACATGGGAGACATCTGCCTTTTCAAAGGACAGCCTTATGCCACCGACAAATTTGGTAAGACAATTATGGTTGGACTGGATGATGACTCGAGTGTCCAATTAGTGGCTGAACCATTGGTTGATGGTGGGGACATGAAATTCTTAGTGGAGAGCGAGGGTGATTTGTTGTTAGTAGATGTTTATGAGTGTATACGTACTGGGTTCCCTGATCATGATCCTGTAAGGATTGATGTGTTTAAGCTTAATGAGAAGGAGAAGAAGTGGGTGAAGTTGACTAGTTTAGGGGACAGGGTTTTGTTTTTGAACTTTATATGCTCATTTTCTGCATCTGCTTCAGATTTGTGTGTTTCCAAAGGAAATTGTGTTATCTTTATGGATTATCTCTTCACATCAGATATCTACGATGGACGAAATACATGTGTTTTGCACTTGGATCAAGGTCGACTATCATCTGTGTCTGATTCTCCTGAATTTTCCAACTTGTTCTGGCCACCTCCCGATCACAAAAAGAAAAACATGAAGAAACACTCTAGCTATGGTATTGTTTATGCAGTAGTGGTTGTGGCAATAGTGTTTTTGGGGGAGGAGACTTACATGGCAAAGGCATTGACTTGTAGCCCTGTAGAGTTCAGTCCTTGTTTAGGATCAATCACAACTTCTTCTCCACCAACAAGTGCTTGTTGCCAGAAGTTAAGAGAGCAGAGACCATGCCTTTGTGGTTACCTCAAGAATCCAAGCCTCAAGCAGTATATTTACTCTCCTGGTGCTAGAAGGGTTACTAGCTCTTGTGGGGTTCCCTTCCCAACTTGTTAG |
| Protein sequence |
>MsG0680030303.01.T01 MDNYDTTFQYNVNYIGPFKVVAVTCHGRRPLIVGTLNCVPQPILLKCRNDENWKVIPDDMSIDFYMGDICLFKGQPYATDKFGKTIMVGLDDDSSVQLVAEPLVDGGDMKFLVESEGDLLLVDVYECIRTGFPDHDPVRIDVFKLNEKEKKWVKLTSLGDRVLFLNFICSFSASASDLCVSKGNCVIFMDYLFTSDIYDGRNTCVLHLDQGRLSSVSDSPEFSNLFWPPPDHKKKNMKKHSSYGIVYAVVVVAIVFLGEETYMAKALTCSPVEFSPCLGSITTSSPPTSACCQKLREQRPCLCGYLKNPSLKQYIYSPGARRVTSSCGVPFPTC* |
