AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046724.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046724.01.T02 MTR_4g113750 92.784 97 7 0 1 97 1 97 8.54e-64 189
MsG0880046724.01.T01 MTR_4g113750 96.262 107 4 0 1 107 1 107 1.03e-73 214
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046724.01.T02 AT1G75125 56.471 85 37 0 13 97 11 95 1.42e-32 110
MsG0880046724.01.T01 AT1G75125 60.638 94 37 0 13 106 11 104 1.20e-39 128

Find 23 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGCTTTCATTTGAATATT+GGG 0.195211 8:+77381260 MsG0880046724.01.T01:intron
GTCCTACTTGCGATGGAAAC+AGG 0.354255 8:+77381209 MsG0880046724.01.T01:CDS
AATCTTTACCCGTTTATTCT+CGG 0.396090 8:-77380978 None:intergenic
CGATTTCCAACATGGATTAC+CGG 0.398072 8:+77380878 MsG0880046724.01.T01:CDS
ACAACGTTGTCTGAATTGTC+TGG 0.418889 8:+77381349 MsG0880046724.01.T01:CDS
ACACATGGGTTAATTGCAAC+AGG 0.458488 8:-77381184 None:intergenic
TTGCTTTCATTTGAATATTG+GGG 0.481504 8:+77381261 MsG0880046724.01.T01:intron
TGTCTGAATTGTCTGGGAAA+AGG 0.496121 8:+77381356 MsG0880046724.01.T01:CDS
ATATATGCAAGTTATGCAGC+GGG 0.546291 8:+77381128 MsG0880046724.01.T01:CDS
CAACGTTGTCTGAATTGTCT+GGG 0.565752 8:+77381350 MsG0880046724.01.T01:CDS
GCGGGAATTCAACCATAGCT+TGG 0.577529 8:+77381146 MsG0880046724.01.T01:CDS
TGTAAAAGTTGTAGAGGGAA+AGG 0.582354 8:+77381100 MsG0880046724.01.T01:CDS
CCCCAGTATTACAAAAGCAC+CGG 0.584681 8:-77380897 None:intergenic
TGTGTTTGTCCTACTTGCGA+TGG 0.598415 8:+77381202 MsG0880046724.01.T01:CDS
TTGCCGTGTAAAAGTTGTAG+AGG 0.598560 8:+77381094 MsG0880046724.01.T01:CDS
ATTGCAACAGGATCATACAT+TGG 0.599710 8:-77381172 None:intergenic
ATACATTGGAGACCAAGCTA+TGG 0.629079 8:-77381158 None:intergenic
TCGCAAGTAGGACAAACACA+TGG 0.629902 8:-77381199 None:intergenic
GGGAATTGCAACTCTGAACG+TGG 0.644672 8:+77380919 MsG0880046724.01.T01:CDS
TATATATGCAAGTTATGCAG+CGG 0.648101 8:+77381127 MsG0880046724.01.T01:CDS
TGCCGTGTAAAAGTTGTAGA+GGG 0.654823 8:+77381095 MsG0880046724.01.T01:CDS
CGCAAGTAGGACAAACACAT+GGG 0.656170 8:-77381198 None:intergenic
AACCTGTTTCCATCGCAAGT+AGG 0.668457 8:-77381211 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATTATAGTAACTGATAA+TGG + Chr8:77381312-77381331 MsG0880046724.01.T01:intron 20.0%
!! TTTGCTTTCATTTGAATATT+GGG + Chr8:77381260-77381279 MsG0880046724.01.T01:intron 20.0%
!!! TTTTGCTTTCATTTGAATAT+TGG + Chr8:77381259-77381278 MsG0880046724.01.T01:intron 20.0%
! TTGCTTTCATTTGAATATTG+GGG + Chr8:77381261-77381280 MsG0880046724.01.T01:intron 25.0%
AATCTTTACCCGTTTATTCT+CGG - Chr8:77380981-77381000 None:intergenic 30.0%
GATAATATTGATGTGCTTTC+TGG + Chr8:77381037-77381056 MsG0880046724.01.T01:intron 30.0%
TATATATGCAAGTTATGCAG+CGG + Chr8:77381127-77381146 MsG0880046724.01.T01:CDS 30.0%
TGTCTTGAAATTGAATTGCA+AGG + Chr8:77381290-77381309 MsG0880046724.01.T01:intron 30.0%
ACTTTGTGTCCGAGAATAAA+CGG + Chr8:77380969-77380988 MsG0880046724.01.T01:CDS 35.0%
ATATATGCAAGTTATGCAGC+GGG + Chr8:77381128-77381147 MsG0880046724.01.T01:CDS 35.0%
ATTGCAACAGGATCATACAT+TGG - Chr8:77381175-77381194 None:intergenic 35.0%
TGTAAAAGTTGTAGAGGGAA+AGG + Chr8:77381100-77381119 MsG0880046724.01.T01:CDS 35.0%
! TTCCCTCTACAACTTTTACA+CGG - Chr8:77381100-77381119 None:intergenic 35.0%
! TTGATGTGCTTTCTGGAAAT+TGG + Chr8:77381044-77381063 MsG0880046724.01.T01:intron 35.0%
!!! ACTGATAATGGTTTTGTTGC+AGG + Chr8:77381324-77381343 MsG0880046724.01.T01:intron 35.0%
!!! CTGATAATGGTTTTGTTGCA+GGG + Chr8:77381325-77381344 MsG0880046724.01.T01:intron 35.0%
ACAACGTTGTCTGAATTGTC+TGG + Chr8:77381349-77381368 MsG0880046724.01.T01:CDS 40.0%
ATACATTGGAGACCAAGCTA+TGG - Chr8:77381161-77381180 None:intergenic 40.0%
CAACGTTGTCTGAATTGTCT+GGG + Chr8:77381350-77381369 MsG0880046724.01.T01:CDS 40.0%
CTTTGTGTCCGAGAATAAAC+GGG + Chr8:77380970-77380989 MsG0880046724.01.T01:CDS 40.0%
TGCCGTGTAAAAGTTGTAGA+GGG + Chr8:77381095-77381114 MsG0880046724.01.T01:CDS 40.0%
TGTCTGAATTGTCTGGGAAA+AGG + Chr8:77381356-77381375 MsG0880046724.01.T01:CDS 40.0%
TTGCCGTGTAAAAGTTGTAG+AGG + Chr8:77381094-77381113 MsG0880046724.01.T01:CDS 40.0%
! ACACATGGGTTAATTGCAAC+AGG - Chr8:77381187-77381206 None:intergenic 40.0%
! ACCGGTGCTTTTGTAATACT+GGG + Chr8:77380896-77380915 MsG0880046724.01.T01:CDS 40.0%
! CGATTTCCAACATGGATTAC+CGG + Chr8:77380878-77380897 MsG0880046724.01.T01:CDS 40.0%
! TACCGGTGCTTTTGTAATAC+TGG + Chr8:77380895-77380914 MsG0880046724.01.T01:CDS 40.0%
AACCTGTTTCCATCGCAAGT+AGG - Chr8:77381214-77381233 None:intergenic 45.0%
ATCCATGTTGGAAATCGCCT+TGG - Chr8:77380875-77380894 None:intergenic 45.0%
CGCAAGTAGGACAAACACAT+GGG - Chr8:77381201-77381220 None:intergenic 45.0%
GTTGGAAATCGCCTTGGAAT+TGG - Chr8:77380869-77380888 None:intergenic 45.0%
TCGCAAGTAGGACAAACACA+TGG - Chr8:77381202-77381221 None:intergenic 45.0%
TGTGTTTGTCCTACTTGCGA+TGG + Chr8:77381202-77381221 MsG0880046724.01.T01:CDS 45.0%
TTCCAAGGCGATTTCCAACA+TGG + Chr8:77380870-77380889 MsG0880046724.01.T01:CDS 45.0%
! CCCCAGTATTACAAAAGCAC+CGG - Chr8:77380900-77380919 None:intergenic 45.0%
! CCGGTGCTTTTGTAATACTG+GGG + Chr8:77380897-77380916 MsG0880046724.01.T01:CDS 45.0%
! CGGTGCTTTTGTAATACTGG+GGG + Chr8:77380898-77380917 MsG0880046724.01.T01:CDS 45.0%
! GGTGCTTTTGTAATACTGGG+GGG + Chr8:77380899-77380918 MsG0880046724.01.T01:CDS 45.0%
!! AAAGCACCGGTAATCCATGT+TGG - Chr8:77380887-77380906 None:intergenic 45.0%
GCGGGAATTCAACCATAGCT+TGG + Chr8:77381146-77381165 MsG0880046724.01.T01:CDS 50.0%
GGGAATTGCAACTCTGAACG+TGG + Chr8:77380919-77380938 MsG0880046724.01.T01:CDS 50.0%
GTCCTACTTGCGATGGAAAC+AGG + Chr8:77381209-77381228 MsG0880046724.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr8 gene 77380860 77381388 77380860 ID=MsG0880046724.01;Name=MsG0880046724.01
Chr8 mRNA 77380860 77381388 77380860 ID=MsG0880046724.01.T01;Parent=MsG0880046724.01;Name=MsG0880046724.01.T01;_AED=0.29;_eAED=0.30;_QI=0|0|0|1|0|0|3|0|107
Chr8 exon 77381081 77381230 77381081 ID=MsG0880046724.01.T01:exon:32773;Parent=MsG0880046724.01.T01
Chr8 exon 77381346 77381388 77381346 ID=MsG0880046724.01.T01:exon:32774;Parent=MsG0880046724.01.T01
Chr8 CDS 77380860 77380990 77380860 ID=MsG0880046724.01.T01:cds;Parent=MsG0880046724.01.T01
Chr8 CDS 77381081 77381230 77381081 ID=MsG0880046724.01.T01:cds;Parent=MsG0880046724.01.T01
Chr8 CDS 77381346 77381388 77381346 ID=MsG0880046724.01.T01:cds;Parent=MsG0880046724.01.T01
Chr8 mRNA 77380860 77381297 77380860 ID=MsG0880046724.01.T02;Parent=MsG0880046724.01;Name=MsG0880046724.01.T02;_AED=0.28;_eAED=0.40;_QI=0|0|0|1|0|0|2|0|115
Chr8 exon 77381081 77381297 77381081 ID=MsG0880046724.01.T02:exon:32775;Parent=MsG0880046724.01.T02
Chr8 CDS 77380860 77380990 77380860 ID=MsG0880046724.01.T02:cds;Parent=MsG0880046724.01.T02
Chr8 CDS 77381081 77381297 77381081 ID=MsG0880046724.01.T02:cds;Parent=MsG0880046724.01.T02
Chr8 mRNA 77380889 77380987 77380889 ID=MsG0880046724.01.T03;Parent=MsG0880046724.01;Name=MsG0880046724.01.T03;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|0|1|0|32
Chr8 exon 77380889 77380987 77380889 ID=MsG0880046724.01.T03:exon:32776;Parent=MsG0880046724.01.T03
Chr8 CDS 77380889 77380987 77380889 ID=MsG0880046724.01.T03:cds;Parent=MsG0880046724.01.T03
Gene Sequence

>MsG0880046724.01.T02

ATGAATCCAATTCCAAGGCGATTTCCAACATGGATTACCGGTGCTTTTGTAATACTGGGGGGAATTGCAACTCTGAACGTGGCTTCTACACTTACACTCAAAACACTTCACTTTGTGTCCGAGAATAAACGAAAAAAAACAGCGTTGCCGTGTAAAAGTTGTAGAGGGAAAGGTTTTTATATATGCAAGTTATGCAGCGGGAATTCAACCATAGCTTGGTCTCCAATGTATGATCCTGTTGCAATTAACCCATGTGTTTGTCCTACTTGCGATGGAAACAGGTTAGCTAAGTCTTTTTTCATTCTCTCATTTTGCTTTCATTTGAATATTGGGGTTCTCTTGTCTTGA

>MsG0880046724.01.T03

ATGGATTACCGGTGCTTTTGTAATACTGGGGGGAATTGCAACTCTGAACGTGGCTTCTACACTTACACTCAAAACACTTCACTTTGTGTCCGAGAATAA

>MsG0880046724.01.T01

ATGAATCCAATTCCAAGGCGATTTCCAACATGGATTACCGGTGCTTTTGTAATACTGGGGGGAATTGCAACTCTGAACGTGGCTTCTACACTTACACTCAAAACACTTCACTTTGTGTCCGAGAATAAACGAAAAAAAACAGCGTTGCCGTGTAAAAGTTGTAGAGGGAAAGGTTTTTATATATGCAAGTTATGCAGCGGGAATTCAACCATAGCTTGGTCTCCAATGTATGATCCTGTTGCAATTAACCCATGTGTTTGTCCTACTTGCGATGGAAACAGGGTACAACGTTGTCTGAATTGTCTGGGAAAAGGCTATGACTAA

Protein sequence

>MsG0880046724.01.T02

MNPIPRRFPTWITGAFVILGGIATLNVASTLTLKTLHFVSENKRKKTALPCKSCRGKGFYICKLCSGNSTIAWSPMYDPVAINPCVCPTCDGNRLAKSFFILSFCFHLNIGVLLS*

>MsG0880046724.01.T03

MDYRCFCNTGGNCNSERGFYTYTQNTSLCVRE*

>MsG0880046724.01.T01

MNPIPRRFPTWITGAFVILGGIATLNVASTLTLKTLHFVSENKRKKTALPCKSCRGKGFYICKLCSGNSTIAWSPMYDPVAINPCVCPTCDGNRVQRCLNCLGKGYD*