AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023295.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023295.01.T01 MTR_8g089995 93.258 89 6 0 3 91 202 290 6.10e-51 161
MsG0480023295.01.T01 MTR_3g086990 71.429 70 20 0 3 72 195 264 2.15e-26 97.8
MsG0480023295.01.T01 MTR_8g089995 97.297 37 1 0 3 39 202 238 5.02e-16 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023295.01.T01 AT5G65250 77.586 58 13 0 3 60 214 271 3.05e-22 87.8

Find 49 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCATTTACTTTCATTTC+AGG 0.176402 4:+83437877 MsG0480023295.01.T01:three_prime_UTR
CTGGTTCTGTGGTAAATTTC+GGG 0.222924 4:+83437830 MsG0480023295.01.T01:three_prime_UTR
TTTAACCCAAGCTTCAAATT+TGG 0.231116 4:-83437576 None:intergenic
AGCCCTGCAATCAGACATTT+TGG 0.237492 4:+83436959 MsG0480023295.01.T01:CDS
CCGTGTTCTTCACTAGTTTC+AGG 0.242419 4:-83437496 None:intergenic
TTTATGTGCCAATAAAATTC+TGG 0.256690 4:-83437702 None:intergenic
TCTGGTTCTGTGGTAAATTT+CGG 0.258448 4:+83437829 MsG0480023295.01.T01:three_prime_UTR
GGCTAATGCTCTTGCGGCCT+CGG 0.289526 4:-83436940 None:intergenic
TTCATCCCTCTGTAATTTGA+TGG 0.314171 4:-83437609 None:intergenic
ACTTTGACTTTGTGTGTAGA+TGG 0.353715 4:-83437677 None:intergenic
TAGATTAGTAACTGAGCTAT+TGG 0.353976 4:+83438019 MsG0480023295.01.T01:three_prime_UTR
TCTCCAAAATGTCTGATTGC+AGG 0.356336 4:-83436962 None:intergenic
AGATTAGTAACTGAGCTATT+GGG 0.356741 4:+83438020 MsG0480023295.01.T01:three_prime_UTR
GTCCACCAAATTTGAAGCTT+GGG 0.368340 4:+83437571 MsG0480023295.01.T01:exon
TTGAAGCTTGGGTTAAACTA+AGG 0.401346 4:+83437582 MsG0480023295.01.T01:exon
ATGAGATAGGATTGAATTCA+TGG 0.407376 4:+83438059 MsG0480023295.01.T01:three_prime_UTR
TTGAATGAAGACTGCAGCTC+TGG 0.409148 4:+83436905 None:intergenic
CGGGAAACTTGAGGCGTCCT+TGG 0.418063 4:-83437742 None:intergenic
GGTCCACCAAATTTGAAGCT+TGG 0.435933 4:+83437570 MsG0480023295.01.T01:exon
GAATCTCAATGCGGTAAATC+AGG 0.435977 4:+83437546 MsG0480023295.01.T01:CDS
AACCCAAGCTTCAAATTTGG+TGG 0.451264 4:-83437573 None:intergenic
TTGCGAAGGCAGGTAAGCTA+TGG 0.479688 4:+83437464 MsG0480023295.01.T01:CDS
ATTCTTGCAATTGCGAAGGC+AGG 0.502036 4:+83437454 MsG0480023295.01.T01:CDS
TAGGATTGAATTCATGGTGG+TGG 0.502520 4:+83438065 MsG0480023295.01.T01:three_prime_UTR
GGAGAATTGTCTGAACTCTC+TGG 0.503072 4:+83437811 MsG0480023295.01.T01:three_prime_UTR
TTACTCAACGAGTCAATTCA+AGG 0.519358 4:+83437785 MsG0480023295.01.T01:three_prime_UTR
CTCCAAAATGTCTGATTGCA+GGG 0.525218 4:-83436961 None:intergenic
ACTTTCATTTCAGGATTACA+AGG 0.536487 4:+83437886 MsG0480023295.01.T01:three_prime_UTR
AAGTTTCCCGTGGATACAGA+TGG 0.540326 4:+83437755 MsG0480023295.01.T01:three_prime_UTR
GATTAGTAACTGAGCTATTG+GGG 0.561521 4:+83438021 MsG0480023295.01.T01:three_prime_UTR
GGAAAACCATCAAATTACAG+AGG 0.566530 4:+83437603 MsG0480023295.01.T01:three_prime_UTR
GAAAACCATCAAATTACAGA+GGG 0.582046 4:+83437604 MsG0480023295.01.T01:three_prime_UTR
TGCGAAGGCAGGTAAGCTAT+GGG 0.592521 4:+83437465 MsG0480023295.01.T01:CDS
GTCTGAACTCTCTGGTTCTG+TGG 0.592692 4:+83437819 MsG0480023295.01.T01:three_prime_UTR
TCAACGAGTCAATTCAAGGA+GGG 0.615226 4:+83437789 MsG0480023295.01.T01:three_prime_UTR
CAACGAGTCAATTCAAGGAG+GGG 0.615962 4:+83437790 MsG0480023295.01.T01:three_prime_UTR
CTGTATCCACGGGAAACTTG+AGG 0.617797 4:-83437751 None:intergenic
CAGATGAATATACATTGGTG+TGG 0.620848 4:+83437966 MsG0480023295.01.T01:three_prime_UTR
TTGCAGGGCTAATGCTCTTG+CGG 0.622054 4:-83436946 None:intergenic
AGTTTCCCGTGGATACAGAT+GGG 0.625808 4:+83437756 MsG0480023295.01.T01:three_prime_UTR
TCTCATTCTTGCAATTGCGA+AGG 0.648334 4:+83437450 MsG0480023295.01.T01:CDS
TAATGATTACACATTTACCA+AGG 0.648591 4:+83437725 MsG0480023295.01.T01:three_prime_UTR
AGGACGCCTCAAGTTTCCCG+TGG 0.668545 4:+83437745 MsG0480023295.01.T01:three_prime_UTR
TCTCAATGCGGTAAATCAGG+AGG 0.687985 4:+83437549 MsG0480023295.01.T01:CDS
CCTGAAACTAGTGAAGAACA+CGG 0.696745 4:+83437496 MsG0480023295.01.T01:CDS
CTCAACGAGTCAATTCAAGG+AGG 0.701887 4:+83437788 MsG0480023295.01.T01:three_prime_UTR
TCTGGCACAGAAGAATTCCG+AGG 0.724089 4:+83436923 MsG0480023295.01.T01:CDS
CTCAGCTGCGAATCTCAATG+CGG 0.734213 4:+83437537 MsG0480023295.01.T01:CDS
AGATAGGATTGAATTCATGG+TGG 0.761795 4:+83438062 MsG0480023295.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACAGATGAATATACAT+TGG + Chr4:83437961-83437980 MsG0480023295.01.T01:three_prime_UTR 20.0%
!!! TAAAGGTTAACATTATTTTG+TGG + Chr4:83437112-83437131 MsG0480023295.01.T01:intron 20.0%
! AGAGTTTAAGTAACATTGAA+GGG - Chr4:83437037-83437056 None:intergenic 25.0%
! TAATGATTACACATTTACCA+AGG + Chr4:83437725-83437744 MsG0480023295.01.T01:three_prime_UTR 25.0%
! TGTTCATTTACTTTCATTTC+AGG + Chr4:83437877-83437896 MsG0480023295.01.T01:three_prime_UTR 25.0%
! TTTATGTGCCAATAAAATTC+TGG - Chr4:83437705-83437724 None:intergenic 25.0%
!! TTTTTGTTACAGCAAAACTA+AGG - Chr4:83437946-83437965 None:intergenic 25.0%
!!! AAGTTTGTTTTGTATGAGAT+AGG + Chr4:83438046-83438065 MsG0480023295.01.T01:three_prime_UTR 25.0%
!!! AGTTTGTTAGTGAACTTTAA+AGG + Chr4:83437095-83437114 MsG0480023295.01.T01:intron 25.0%
AAACTGGAATACTAGATTCA+TGG - Chr4:83437287-83437306 None:intergenic 30.0%
ACTTTCATTTCAGGATTACA+AGG + Chr4:83437886-83437905 MsG0480023295.01.T01:three_prime_UTR 30.0%
AGATTAGTAACTGAGCTATT+GGG + Chr4:83438020-83438039 MsG0480023295.01.T01:three_prime_UTR 30.0%
ATGAGATAGGATTGAATTCA+TGG + Chr4:83438059-83438078 MsG0480023295.01.T01:three_prime_UTR 30.0%
CAGAGTTTAAGTAACATTGA+AGG - Chr4:83437038-83437057 None:intergenic 30.0%
GAAAACCATCAAATTACAGA+GGG + Chr4:83437604-83437623 MsG0480023295.01.T01:three_prime_UTR 30.0%
TAGATTAGTAACTGAGCTAT+TGG + Chr4:83438019-83438038 MsG0480023295.01.T01:three_prime_UTR 30.0%
TTTAACCCAAGCTTCAAATT+TGG - Chr4:83437579-83437598 None:intergenic 30.0%
! CAAAGTAGCCAGAATTTTAT+TGG + Chr4:83437694-83437713 MsG0480023295.01.T01:three_prime_UTR 30.0%
!! CTAGTGTCATTTGATTTCTA+AGG - Chr4:83437077-83437096 None:intergenic 30.0%
AAAATCTCTAGTCTGACGTA+TGG - Chr4:83437314-83437333 None:intergenic 35.0%
AAATCTCTAGTCTGACGTAT+GGG - Chr4:83437313-83437332 None:intergenic 35.0%
AGATAGGATTGAATTCATGG+TGG + Chr4:83438062-83438081 MsG0480023295.01.T01:three_prime_UTR 35.0%
CAGATGAATATACATTGGTG+TGG + Chr4:83437966-83437985 MsG0480023295.01.T01:three_prime_UTR 35.0%
GATTAGTAACTGAGCTATTG+GGG + Chr4:83438021-83438040 MsG0480023295.01.T01:three_prime_UTR 35.0%
GGAAAACCATCAAATTACAG+AGG + Chr4:83437603-83437622 MsG0480023295.01.T01:three_prime_UTR 35.0%
TTACTCAACGAGTCAATTCA+AGG + Chr4:83437785-83437804 MsG0480023295.01.T01:three_prime_UTR 35.0%
TTCATCCCTCTGTAATTTGA+TGG - Chr4:83437612-83437631 None:intergenic 35.0%
TTGAAGCTTGGGTTAAACTA+AGG + Chr4:83437582-83437601 MsG0480023295.01.T01:exon 35.0%
! ACTTTGACTTTGTGTGTAGA+TGG - Chr4:83437680-83437699 None:intergenic 35.0%
!! TCTGGTTCTGTGGTAAATTT+CGG + Chr4:83437829-83437848 MsG0480023295.01.T01:three_prime_UTR 35.0%
!!! TTTTTTCCCATCTGTATCCA+CGG - Chr4:83437765-83437784 None:intergenic 35.0%
AACCCAAGCTTCAAATTTGG+TGG - Chr4:83437576-83437595 None:intergenic 40.0%
CCTGAAACTAGTGAAGAACA+CGG + Chr4:83437496-83437515 MsG0480023295.01.T01:CDS 40.0%
CTCCAAAATGTCTGATTGCA+GGG - Chr4:83436964-83436983 None:intergenic 40.0%
CTTAAACTCTGTGAAAGAGC+AGG + Chr4:83437047-83437066 MsG0480023295.01.T01:intron 40.0%
GAATCTCAATGCGGTAAATC+AGG + Chr4:83437546-83437565 MsG0480023295.01.T01:CDS 40.0%
GACATCATTGTGTTGTGTGT+GGG - Chr4:83437229-83437248 None:intergenic 40.0%
TAGGATTGAATTCATGGTGG+TGG + Chr4:83438065-83438084 MsG0480023295.01.T01:three_prime_UTR 40.0%
TCAACGAGTCAATTCAAGGA+GGG + Chr4:83437789-83437808 MsG0480023295.01.T01:three_prime_UTR 40.0%
TCTCATTCTTGCAATTGCGA+AGG + Chr4:83437450-83437469 MsG0480023295.01.T01:CDS 40.0%
TCTCCAAAATGTCTGATTGC+AGG - Chr4:83436965-83436984 None:intergenic 40.0%
TCTGACGTATGGGTTTAAAC+TGG - Chr4:83437303-83437322 None:intergenic 40.0%
TGACATCATTGTGTTGTGTG+TGG - Chr4:83437230-83437249 None:intergenic 40.0%
TGGTGTTTGATCGTTCATGT+AGG + Chr4:83437405-83437424 MsG0480023295.01.T01:intron 40.0%
! AAAGGTTTTGCTAGCAATGC+AGG + Chr4:83437004-83437023 MsG0480023295.01.T01:CDS 40.0%
! GTCCACCAAATTTGAAGCTT+GGG + Chr4:83437571-83437590 MsG0480023295.01.T01:exon 40.0%
! TTTTTCCCATCTGTATCCAC+GGG - Chr4:83437764-83437783 None:intergenic 40.0%
!! CTGGTTCTGTGGTAAATTTC+GGG + Chr4:83437830-83437849 MsG0480023295.01.T01:three_prime_UTR 40.0%
!! GCAATCAGACATTTTGGAGA+AGG + Chr4:83436965-83436984 MsG0480023295.01.T01:CDS 40.0%
!! TCGAGTTTGACTTGTCTTGA+TGG + Chr4:83437385-83437404 MsG0480023295.01.T01:intron 40.0%
AAGTTTCCCGTGGATACAGA+TGG + Chr4:83437755-83437774 MsG0480023295.01.T01:three_prime_UTR 45.0%
AGTTTCCCGTGGATACAGAT+GGG + Chr4:83437756-83437775 MsG0480023295.01.T01:three_prime_UTR 45.0%
ATTCTTGCAATTGCGAAGGC+AGG + Chr4:83437454-83437473 MsG0480023295.01.T01:CDS 45.0%
CAACGAGTCAATTCAAGGAG+GGG + Chr4:83437790-83437809 MsG0480023295.01.T01:three_prime_UTR 45.0%
CTCAACGAGTCAATTCAAGG+AGG + Chr4:83437788-83437807 MsG0480023295.01.T01:three_prime_UTR 45.0%
GGAGAATTGTCTGAACTCTC+TGG + Chr4:83437811-83437830 MsG0480023295.01.T01:three_prime_UTR 45.0%
TCTCAATGCGGTAAATCAGG+AGG + Chr4:83437549-83437568 MsG0480023295.01.T01:CDS 45.0%
! AGCCCTGCAATCAGACATTT+TGG + Chr4:83436959-83436978 MsG0480023295.01.T01:CDS 45.0%
! CCGTGTTCTTCACTAGTTTC+AGG - Chr4:83437499-83437518 None:intergenic 45.0%
! GGTCCACCAAATTTGAAGCT+TGG + Chr4:83437570-83437589 MsG0480023295.01.T01:exon 45.0%
!! ACATTTTGGAGAAGGAGCTG+GGG + Chr4:83436973-83436992 MsG0480023295.01.T01:CDS 45.0%
!! AGACATTTTGGAGAAGGAGC+TGG + Chr4:83436971-83436990 MsG0480023295.01.T01:CDS 45.0%
!! GACATTTTGGAGAAGGAGCT+GGG + Chr4:83436972-83436991 MsG0480023295.01.T01:CDS 45.0%
CTCAGCTGCGAATCTCAATG+CGG + Chr4:83437537-83437556 MsG0480023295.01.T01:CDS 50.0%
CTGTATCCACGGGAAACTTG+AGG - Chr4:83437754-83437773 None:intergenic 50.0%
TCTGGCACAGAAGAATTCCG+AGG + Chr4:83436923-83436942 MsG0480023295.01.T01:CDS 50.0%
TGCGAAGGCAGGTAAGCTAT+GGG + Chr4:83437465-83437484 MsG0480023295.01.T01:CDS 50.0%
TTGCAGGGCTAATGCTCTTG+CGG - Chr4:83436949-83436968 None:intergenic 50.0%
TTGCGAAGGCAGGTAAGCTA+TGG + Chr4:83437464-83437483 MsG0480023295.01.T01:CDS 50.0%
!! CATTTTGGAGAAGGAGCTGG+GGG + Chr4:83436974-83436993 MsG0480023295.01.T01:CDS 50.0%
!! GTCTGAACTCTCTGGTTCTG+TGG + Chr4:83437819-83437838 MsG0480023295.01.T01:three_prime_UTR 50.0%
GGAGCTGGGGGAAGTTCAAA+AGG + Chr4:83436986-83437005 MsG0480023295.01.T01:CDS 55.0%
TGTTGTGTGTGGGTGTGCGT+AGG - Chr4:83437219-83437238 None:intergenic 55.0%
AGGACGCCTCAAGTTTCCCG+TGG + Chr4:83437745-83437764 MsG0480023295.01.T01:three_prime_UTR 60.0%
GGCTAATGCTCTTGCGGCCT+CGG - Chr4:83436943-83436962 None:intergenic 60.0%
GTGTGTGGGTGTGCGTAGGT+GGG - Chr4:83437215-83437234 None:intergenic 60.0%
TGTGTGTGGGTGTGCGTAGG+TGG - Chr4:83437216-83437235 None:intergenic 60.0%
! CGGGAAACTTGAGGCGTCCT+TGG - Chr4:83437745-83437764 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 83436909 83438095 83436909 ID=MsG0480023295.01;Name=MsG0480023295.01
Chr4 mRNA 83436909 83438095 83436909 ID=MsG0480023295.01.T01;Parent=MsG0480023295.01;Name=MsG0480023295.01.T01;_AED=0.46;_eAED=0.46;_QI=0|1|0.5|1|1|1|2|510|91
Chr4 exon 83436909 83437025 83436909 ID=MsG0480023295.01.T01:exon:1109;Parent=MsG0480023295.01.T01
Chr4 exon 83437427 83438095 83437427 ID=MsG0480023295.01.T01:exon:1110;Parent=MsG0480023295.01.T01
Chr4 CDS 83436909 83437025 83436909 ID=MsG0480023295.01.T01:cds;Parent=MsG0480023295.01.T01
Chr4 CDS 83437427 83437585 83437427 ID=MsG0480023295.01.T01:cds;Parent=MsG0480023295.01.T01
Chr4 three_prime_UTR 83437586 83438095 83437586 ID=MsG0480023295.01.T01:three_prime_utr;Parent=MsG0480023295.01.T01
Gene Sequence

>MsG0480023295.01.T01

ATGAAGACTGCAGCTCTGGCACAGAAGAATTCCGAGGCCGCAAGAGCATTAGCCCTGCAATCAGACATTTTGGAGAAGGAGCTGGGGGAAGTTCAAAAGGTTTTGCTAGCAATGCAGGAACAACAGCAAAAACAACTTGATCTCATTCTTGCAATTGCGAAGGCAGGTAAGCTATGGGAAAGCAAGCCTGAAACTAGTGAAGAACACGGTACAATAGAAATGTCTAACTCAGCTGCGAATCTCAATGCGGTAAATCAGGAGGTCCACCAAATTTGA

Protein sequence

>MsG0480023295.01.T01

MKTAALAQKNSEAARALALQSDILEKELGEVQKVLLAMQEQQQKQLDLILAIAKAGKLWESKPETSEEHGTIEMSNSAANLNAVNQEVHQI*