AlfalfaGEDB | MsG0580025497.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025497.01

KEGG: K11971
GO: GO:0005515
PFAM: PF01485 PF01535 PF05773 PF12854 PF13041

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025497.01.T01	XP_003612847.1	91.969	523	39	2	1	523	19	538	0	984

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025497.01.T01	Q9JI90	30.82	451	261	12	113	522	7	447	4.80E-56	205

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025497.01.T01	G7KDN8	91.969	523	39	2	1	523	19	538	0.0	984

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048177.01	MsG0580025497.01	0.808223	3.441052e-50	1.956514e-47
MsG0080048178.01	MsG0580025497.01	0.820347	7.197296e-53	5.680498e-50
MsG0080048205.01	MsG0580025497.01	0.823873	1.095675e-53	9.551280e-51
MsG0080048207.01	MsG0580025497.01	0.810884	9.230443e-51	5.630991e-48
MsG0080048309.01	MsG0580025497.01	0.802605	5.181771e-49	2.549087e-46
MsG0080048830.01	MsG0580025497.01	0.810620	1.052630e-50	6.376129e-48
MsG0080048982.01	MsG0580025497.01	0.807939	3.955838e-50	2.232252e-47
MsG0180000391.01	MsG0580025497.01	0.817202	3.725652e-52	2.695762e-49
MsG0180000555.01	MsG0580025497.01	0.804914	1.718517e-49	8.970966e-47
MsG0180000855.01	MsG0580025497.01	0.828375	9.310037e-55	9.237025e-52

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025497.01.T01	MTR_5g029700	91.969	523	39	2	1	523	19	538	0.0	984
MsG0580025497.01.T01	MTR_5g029690	69.620	632	59	4	533	1031	1	632	0.0	842
MsG0580025497.01.T01	MTR_5g029690	31.579	437	233	3	661	1031	267	703	8.23e-60	219
MsG0580025497.01.T01	MTR_5g029690	26.667	180	121	3	643	817	531	704	7.82e-14	76.6
MsG0580025497.01.T01	MTR_5g077270	51.293	464	134	4	655	1051	353	791	6.50e-135	429
MsG0580025497.01.T01	MTR_5g077270	33.788	586	268	17	523	1032	24	565	1.71e-80	281
MsG0580025497.01.T01	MTR_5g077270	27.273	418	226	8	635	1031	274	634	1.25e-30	130
MsG0580025497.01.T01	MTR_5g077270	25.000	304	226	1	674	975	492	795	1.20e-21	102
MsG0580025497.01.T01	MTR_6g478210	32.055	365	235	2	675	1031	2	361	2.64e-54	194
MsG0580025497.01.T01	MTR_6g478210	30.255	314	210	2	652	956	48	361	1.30e-38	148

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025497.01.T01	AT1G32340	57.870	432	176	2	91	522	165	590	0.0	552
MsG0580025497.01.T01	AT1G12775	26.377	599	379	12	500	1043	33	624	3.16e-55	204
MsG0580025497.01.T01	AT1G12775	27.147	361	250	3	630	983	276	630	9.32e-37	148
MsG0580025497.01.T01	AT3G22470	26.627	507	347	6	539	1030	59	555	9.26e-52	193
MsG0580025497.01.T01	AT3G22470	30.240	334	228	2	675	1008	275	603	4.05e-40	158
MsG0580025497.01.T01	AT3G22470	27.778	306	216	1	726	1031	116	416	2.99e-38	152
MsG0580025497.01.T01	AT3G22470	27.070	314	224	1	718	1031	143	451	1.09e-35	145
MsG0580025497.01.T01	AT3G22470	25.581	301	219	1	731	1031	51	346	1.48e-27	119
MsG0580025497.01.T01	AT3G22470	25.200	250	187	0	664	913	369	618	3.00e-23	106
MsG0580025497.01.T01	AT3G22470	27.811	169	122	0	652	820	427	595	7.35e-19	92.4

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Find 182 sgRNAs with CRISPR-Local

Find 492 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACACTTTCTTGGCGACG+AGG	0.633505	5:-19631590	MsG0580025497.01.T01:CDS
AAAATGAGGTGCGGTAATTG+TGG	0.499022	5:-19630140	MsG0580025497.01.T01:CDS
AAAATTCATCTCGTTTAAAG+GGG	0.576489	5:-19630283	MsG0580025497.01.T01:CDS
AAAATTGGAATTTCAGTTTC+AGG	0.187309	5:+19627158	None:intergenic
AAACATGTCAAAACAATCGG+TGG	0.627900	5:+19632302	None:intergenic
AAACTGAAGCTTCATTTCAA+GGG	0.545570	5:+19631381	None:intergenic
AAATTCTTCTCTTTCTCATT+TGG	0.248104	5:-19632120	MsG0580025497.01.T01:CDS
AAATTTCTCCGACAATGCCA+AGG	0.588043	5:+19625940	None:intergenic
AACAATGGTTATTGAGAGGC+TGG	0.406404	5:+19626431	None:intergenic
AACATTAATGCAGAAGAAGA+TGG	0.506759	5:+19627185	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAATCCTCCCTAAAATCT+CGG	+	Chr5:19632556-19632575	None:intergenic	30.0%
AAAAATGTTTGCATGGTAAC+CGG	+	Chr5:19627398-19627417	None:intergenic	30.0%
AAACTGAAGCTTCATTTCAA+GGG	+	Chr5:19627742-19627761	None:intergenic	30.0%
AACATTAATGCAGAAGAAGA+TGG	+	Chr5:19631938-19631957	None:intergenic	30.0%
AATTCTCTACAAAAACTGGA+AGG	+	Chr5:19629507-19629526	None:intergenic	30.0%
ACAGAGATTGTAAATATAGG+CGG	+	Chr5:19626872-19626891	None:intergenic	30.0%
ACAGTTATGTTGTTGATGTA+AGG	+	Chr5:19628598-19628617	None:intergenic	30.0%
ACATCAACAACATAACTGTT+TGG	-	Chr5:19628599-19628618	MsG0580025497.01.T01:intron	30.0%
ACATTCTTAGTATTAACTGC+AGG	-	Chr5:19633138-19633157	MsG0580025497.01.T01:intron	30.0%
ACCTTAGATACATTTCCATT+AGG	-	Chr5:19630054-19630073	MsG0580025497.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	19625356	19633786	19625356	ID=MsG0580025497.01;Name=MsG0580025497.01
Chr5	mRNA	19625356	19633786	19625356	ID=MsG0580025497.01.T01;Parent=MsG0580025497.01;Name=MsG0580025497.01.T01;_AED=0.45;_eAED=0.46;_QI=36\|0.5\|0.55\|1\|1\|1\|9\|0\|1051
Chr5	exon	19625356	19625597	19625356	ID=MsG0580025497.01.T01:exon:22923;Parent=MsG0580025497.01.T01
Chr5	exon	19625669	19626624	19625669	ID=MsG0580025497.01.T01:exon:22922;Parent=MsG0580025497.01.T01
Chr5	exon	19626762	19626843	19626762	ID=MsG0580025497.01.T01:exon:22921;Parent=MsG0580025497.01.T01
Chr5	exon	19626910	19627217	19626910	ID=MsG0580025497.01.T01:exon:22920;Parent=MsG0580025497.01.T01
Chr5	exon	19630077	19630312	19630077	ID=MsG0580025497.01.T01:exon:22919;Parent=MsG0580025497.01.T01
Chr5	exon	19631375	19631760	19631375	ID=MsG0580025497.01.T01:exon:22918;Parent=MsG0580025497.01.T01
Chr5	exon	19631934	19632450	19631934	ID=MsG0580025497.01.T01:exon:22917;Parent=MsG0580025497.01.T01
Chr5	exon	19632562	19632636	19632562	ID=MsG0580025497.01.T01:exon:22916;Parent=MsG0580025497.01.T01
Chr5	exon	19633397	19633786	19633397	ID=MsG0580025497.01.T01:exon:22915;Parent=MsG0580025497.01.T01
Chr5	five_prime_UTR	19633751	19633786	19633751	ID=MsG0580025497.01.T01:five_prime_utr;Parent=MsG0580025497.01.T01
Chr5	CDS	19633397	19633750	19633397	ID=MsG0580025497.01.T01:cds;Parent=MsG0580025497.01.T01
Chr5	CDS	19632562	19632636	19632562	ID=MsG0580025497.01.T01:cds;Parent=MsG0580025497.01.T01
Chr5	CDS	19631934	19632450	19631934	ID=MsG0580025497.01.T01:cds;Parent=MsG0580025497.01.T01
Chr5	CDS	19631375	19631760	19631375	ID=MsG0580025497.01.T01:cds;Parent=MsG0580025497.01.T01
Chr5	CDS	19630077	19630312	19630077	ID=MsG0580025497.01.T01:cds;Parent=MsG0580025497.01.T01
Chr5	CDS	19626910	19627217	19626910	ID=MsG0580025497.01.T01:cds;Parent=MsG0580025497.01.T01
Chr5	CDS	19626762	19626843	19626762	ID=MsG0580025497.01.T01:cds;Parent=MsG0580025497.01.T01
Chr5	CDS	19625669	19626624	19625669	ID=MsG0580025497.01.T01:cds;Parent=MsG0580025497.01.T01
Chr5	CDS	19625356	19625597	19625356	ID=MsG0580025497.01.T01:cds;Parent=MsG0580025497.01.T01

Gene Sequence

>MsG0580025497.01.T01

ATGAATGGTAGTAGATATCACCGCAACAAATCACAACAACAACATCGTACCAAATCGAAACTCCGCTACGTTAACAAATCCGAACTTATTGTTTCTTCACCTAACTCTGCGGCGGTTGAAGAACCAGTTTCCCCTGAGGTTGTTGATGTCAACAGTAATAACAACAAGAAGAACAACACCGTTGATGACGTGGCCAGTTCAAGCAAGACCAAGGAAGAAGATGATTATGATAATAATGATATTGATTTTGGTATGAATAATACTAATAGGCTTGATAAGTTGCTTTGTGATATTCAACAAATTGAATTATCCCAGGAAGAGATCACAATCAATGATCAATTGCAGCAAGATGAGTTACTTGTGGTGGAATCAATTTATCGAGAAAACGTTTTCAGCCTTGATACATGGAAAGGTCTGCGTTGTTTTCAGATACACATAAACATTGATGTTTTGGGTGAAATAGGCATTACTGCCAAGGTTAACTCTGTTAATGAACTTGAGACATTAAGCAACAATTCAGACGACTTCTTATACTCTTTCAAAGTTCAATATCTTCCACCGATTGTTTTGACATGTTTATTACCTAAGTCATATCCGAGTCACCAACCGCCTATATTTACAATCTCTGTTAAGTGGCTGGAATCTGCGAAGATTTTAAGTTTATGTTCCAAGTTGGATTTAATATGGACAGAACAACAAGGGCAGGAAGTGATTTACAGTTGGGTAGAATGGTTACAAAATTCTTCTCTTTCTCATTTGGGATTTGATGAGGAGATTAGATTAGGTCCTTATGGTGTGAATCGTGTTGTGGACGCACGCGTGGTTTCGGGAATTGGATGCATTGATGCTGATATTCCTTTTTTACAAAGCTACAACAATGAGAGACGCCATCAAAACTTCCTTAAAGAATTGCATGAATGTTGTGTTTGCTATAGTGAATATCCAGGTACCGAATTCGTCCGGTTACCATGCAAACATTTTTTTTGCCGCAAATGCTTACAGACCTTTACACAGATACATGTAAAGGAAGGAAACGTTGGTAATCTTCGATGTCTTGATGCAAAATGCAAGGAGATGATTCCACCAGGCCTTTTAAAACACTTTCTTGGCGACGAGGAATACAAACGTTGGGAATCTATGATGTTAGAGAAAACACTTGCATCAATGTCTGATGTTGCTTATTGCCCGAGATGTGAAACACCCTGTATAGAGGAGGAAGACCAACATGCTCAGTGCCCAAAATGCTTTTTTAGCTTTTGTAACCTTTGCAGGGAACGCCGCCATGTTGGCATTGCATGCATGACCCTTGAAATGAAGCTTCAGTTTCTTCAGGATCGTCAAAATTCATCTCGTTTAAAGGGGAATCAAAGGCAAATTGAACTTGCAAAAATCAATGAAATGCTCAGTATCAAAGCAATTCACAGTGATTCCAAGTTGTGCCCTTTTTGTGGTATGGCGATTTCTCGAACTGGAGGTTGTAACAAAATGAGGTGCGGTAATTGTGGAAAGTACTTCTGTTACCTCTGCAACAAAGCACTTGACTCATCAGATCCATATGGACATTACAGGTTCTATTCTCCATCTTCTTCTGCATTAATGTTACAACCTGAAACTGAAATTCCAATTTTCAACGCCCCACTTCCCAAAACCACCACAAAGCTCCCTCGTTTAGTTTCCAAAGTCTTCAAAACCTTAAATTGGGGTGTTGCAAGAGAAATTAAATTCAAGGGTTGTGTTCAAATTCATGGTTTGGAAAATTCAATCAATTCCTTTAGTATCATTATTCACACTTATGCTTTAGCTGGTATGTCTTGGGAAGTTTTTATTTTGCTTAGAGATATTGTTACATTTTATAAGGAAGAGAATCGTGACGTGGTGTTTGCTTCGAATGCTATGCTTGACCATGCTTATTATGTTTTTATTCGTGCTAAGGATACGGAGATTGAGCTGAATATAATAGGTGTTGGGTGTAGTGTTGATATTGGACGTGCGTCCGAGATTTTAGGGAGGATTTTTAGGAGTGGAGAAACCCTGAATGTTGTTACGTATAGTACTTATATTAAGGGACTTTGTAAAGTTGGTTCACCTGGGGTTGCGCGGAGGTTAATTCAAAACTTGTGCCGTAAAAACCAGCCTCTCAATAACCATTGTTTTAATGCTGTCATATATGGATTGTGTCAAAGAGGTCTAGTAGATGAAGCTTCAGAAGTTCTTAAAGAAATGAAGAACTCTGGAATGTTTCCCGATGTTTATAGTTATAGCATTTTAATCAATGGATTTTGCAGGAAAGGTAGAGTAGACAAAGCTTCAGAAGTTTTTAAAGAAATGAGGAACTCTGGAATATTGCCCAATGTTTATAGTTATAGCATTTTAATCGATGGATTTTGCAGGAAAGGTGATATGGATTCAGCCATAAAGATTTGGGAAGAGATGACTAGCAATAATTTTTCTCCTTCTGCTTTTAATTATTGCAGTTTAATTAAGGGGTTATACAAATCCAAACAATTCACTAACGCATTGAAAGAATTTTGGGTCATGCAAAAACACGGCATGTGGCCAGATACAATCGCTTGCAATCACATTCTTAGTATTAACTGCAGGAAGCGCGATTTCAATAATGCCTTGGCATTGTCGGAGAAATTTCAAGAAAATGGAGTTTACTTCAACCCATATTCGTATAATGAATTCATCCACAGGATTTGCAGAGAAAGTGTTCCAGAAAAGGCACTGCAGCTTTTGCCAGTAATGCTGAAAAGGAACGTACTTCCCGATGTTATTAATTATAGTACTCTTATATCATGCTTTGCCAAAAAGTTGAACTTTGAAAAGGCTTGGATGTTATTCACAAAAATGACAAAGGTAGGAATCACTTTCAATGTCAAGACTTATACAATTCTTATTAACCTATTCATCTTTGCTGCTTTTTGTAATACTGGAGAAATGAATAAGGAGGAGGTGTTATTTCATGAAATGTCGCGAGAAGGCTGTTCACCAAATGTAGTTACTTATACCTGTTTCATTAATGCATATTTGAAGTCAAACATGAACGATCAGGCTCATGAGTTGTATAAGAAAATGAAAGAAAGAGGTGTAAAGAGCACAATGAAGGATAGCATAAACGCAATGTCGTGGACCATTTTTTCCTTGGCATTGTGA

Protein sequence

>MsG0580025497.01.T01

MNGSRYHRNKSQQQHRTKSKLRYVNKSELIVSSPNSAAVEEPVSPEVVDVNSNNNKKNNTVDDVASSSKTKEEDDYDNNDIDFGMNNTNRLDKLLCDIQQIELSQEEITINDQLQQDELLVVESIYRENVFSLDTWKGLRCFQIHINIDVLGEIGITAKVNSVNELETLSNNSDDFLYSFKVQYLPPIVLTCLLPKSYPSHQPPIFTISVKWLESAKILSLCSKLDLIWTEQQGQEVIYSWVEWLQNSSLSHLGFDEEIRLGPYGVNRVVDARVVSGIGCIDADIPFLQSYNNERRHQNFLKELHECCVCYSEYPGTEFVRLPCKHFFCRKCLQTFTQIHVKEGNVGNLRCLDAKCKEMIPPGLLKHFLGDEEYKRWESMMLEKTLASMSDVAYCPRCETPCIEEEDQHAQCPKCFFSFCNLCRERRHVGIACMTLEMKLQFLQDRQNSSRLKGNQRQIELAKINEMLSIKAIHSDSKLCPFCGMAISRTGGCNKMRCGNCGKYFCYLCNKALDSSDPYGHYRFYSPSSSALMLQPETEIPIFNAPLPKTTTKLPRLVSKVFKTLNWGVAREIKFKGCVQIHGLENSINSFSIIIHTYALAGMSWEVFILLRDIVTFYKEENRDVVFASNAMLDHAYYVFIRAKDTEIELNIIGVGCSVDIGRASEILGRIFRSGETLNVVTYSTYIKGLCKVGSPGVARRLIQNLCRKNQPLNNHCFNAVIYGLCQRGLVDEASEVLKEMKNSGMFPDVYSYSILINGFCRKGRVDKASEVFKEMRNSGILPNVYSYSILIDGFCRKGDMDSAIKIWEEMTSNNFSPSAFNYCSLIKGLYKSKQFTNALKEFWVMQKHGMWPDTIACNHILSINCRKRDFNNALALSEKFQENGVYFNPYSYNEFIHRICRESVPEKALQLLPVMLKRNVLPDVINYSTLISCFAKKLNFEKAWMLFTKMTKVGITFNVKTYTILINLFIFAAFCNTGEMNKEEVLFHEMSREGCSPNVVTYTCFINAYLKSNMNDQAHELYKKMKERGVKSTMKDSINAMSWTIFSLAL*