Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034445.01.T01 | XP_003619775.1 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 2.43E-74 | 225 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034445.01.T01 | G7KPB8 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 1.16e-74 | 225 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048383.01 | MsG0680034445.01 | 0.803775 | 2.967316e-49 | 1.504107e-46 |
| MsG0180000144.01 | MsG0680034445.01 | 0.811620 | 6.392594e-51 | 3.975918e-48 |
| MsG0180000806.01 | MsG0680034445.01 | 0.812049 | 5.155818e-51 | 3.243636e-48 |
| MsG0180001315.01 | MsG0680034445.01 | 0.801294 | 9.637864e-49 | 4.586053e-46 |
| MsG0180001599.01 | MsG0680034445.01 | 0.811586 | 6.502440e-51 | 4.040663e-48 |
| MsG0180002547.01 | MsG0680034445.01 | 0.816411 | 5.606540e-52 | 3.969700e-49 |
| MsG0180003932.01 | MsG0680034445.01 | 0.800631 | 1.316456e-48 | 6.159936e-46 |
| MsG0180004016.01 | MsG0680034445.01 | 0.805865 | 1.085790e-49 | 5.808065e-47 |
| MsG0180004725.01 | MsG0680034445.01 | 0.815782 | 7.750686e-52 | 5.394680e-49 |
| MsG0180004861.01 | MsG0680034445.01 | 0.813687 | 2.256085e-51 | 1.483330e-48 |
| MsG0180005049.01 | MsG0680034445.01 | 0.834839 | 2.375950e-56 | 2.853673e-53 |
| MsG0180006243.01 | MsG0680034445.01 | 0.824887 | 6.326284e-54 | 5.677068e-51 |
| MsG0380011747.01 | MsG0680034445.01 | 0.817194 | 3.742421e-52 | 2.707155e-49 |
| MsG0380011927.01 | MsG0680034445.01 | 0.823130 | 1.634809e-53 | 1.395506e-50 |
| MsG0380013085.01 | MsG0680034445.01 | 0.807591 | 4.689664e-50 | 2.622349e-47 |
| MsG0380013183.01 | MsG0680034445.01 | 0.800426 | 1.449122e-48 | 6.745721e-46 |
| MsG0380013579.01 | MsG0680034445.01 | 0.806642 | 7.448085e-50 | 4.063562e-47 |
| MsG0680031000.01 | MsG0680034445.01 | 0.814764 | 1.304444e-51 | 8.829675e-49 |
| MsG0680031145.01 | MsG0680034445.01 | 0.809595 | 1.750890e-50 | 1.032349e-47 |
| MsG0680031301.01 | MsG0680034445.01 | 0.816968 | 4.206917e-52 | 3.024755e-49 |
| MsG0680031507.01 | MsG0680034445.01 | 0.812979 | 3.227902e-51 | 2.081785e-48 |
| MsG0680031699.01 | MsG0680034445.01 | 0.816465 | 5.453659e-52 | 3.867036e-49 |
| MsG0680031964.01 | MsG0680034445.01 | 0.818643 | 1.761240e-52 | 1.325765e-49 |
| MsG0680034235.01 | MsG0680034445.01 | 0.803639 | 3.167582e-49 | 1.600015e-46 |
| MsG0680034445.01 | MsG0680034913.01 | 0.825114 | 5.591945e-54 | 5.050320e-51 |
| MsG0680034445.01 | MsG0680035574.01 | 0.801815 | 7.536055e-49 | 3.633470e-46 |
| MsG0680034445.01 | MsG0680035577.01 | 0.808366 | 3.208632e-50 | 1.831254e-47 |
| MsG0680034445.01 | MsG0680035765.01 | 0.800991 | 1.111228e-48 | 5.247777e-46 |
| MsG0680034445.01 | MsG0680035876.01 | 0.810472 | 1.133352e-50 | 6.837960e-48 |
| MsG0680034445.01 | MsG0680035888.01 | 0.825593 | 4.306641e-54 | 3.942874e-51 |
| MsG0680034445.01 | MsG0780036186.01 | 0.811741 | 6.015715e-51 | 3.753648e-48 |
| MsG0680034445.01 | MsG0780036430.01 | 0.807621 | 4.623152e-50 | 2.587255e-47 |
| MsG0680034445.01 | MsG0780039369.01 | 0.803289 | 3.742492e-49 | 1.873445e-46 |
| MsG0680034445.01 | MsG0780040424.01 | 0.807932 | 3.970181e-50 | 2.239897e-47 |
| MsG0680034445.01 | MsG0780040569.01 | 0.831160 | 1.954074e-55 | 2.103013e-52 |
| MsG0680034445.01 | MsG0780040845.01 | 0.820289 | 7.419016e-53 | 5.846071e-50 |
| MsG0680034445.01 | MsG0780041268.01 | 0.810478 | 1.129759e-50 | 6.817311e-48 |
| MsG0680034445.01 | MsG0780041435.01 | 0.804123 | 2.512042e-49 | 1.284799e-46 |
| MsG0680034445.01 | MsG0780041482.01 | 0.827514 | 1.500513e-54 | 1.451730e-51 |
| MsG0680034445.01 | MsG0880042033.01 | 0.864482 | 1.177706e-64 | 3.703360e-61 |
| MsG0680034445.01 | MsG0880042206.01 | 0.808642 | 2.801573e-50 | 1.610354e-47 |
| MsG0680034445.01 | MsG0880042207.01 | 0.804026 | 2.631259e-49 | 1.342461e-46 |
| MsG0680034445.01 | MsG0880043162.01 | 0.809678 | 1.680749e-50 | 9.931514e-48 |
| MsG0680034445.01 | MsG0880044975.01 | 0.803456 | 3.455392e-49 | 1.737227e-46 |
| MsG0680034445.01 | MsG0880044990.01 | 0.802025 | 6.823117e-49 | 3.307289e-46 |
| MsG0680034445.01 | MsG0880045294.01 | 0.800824 | 1.202447e-48 | 5.653866e-46 |
| MsG0680034445.01 | MsG0880045460.01 | 0.812687 | 3.740673e-51 | 2.394084e-48 |
| MsG0680034445.01 | MsG0880045656.01 | 0.849124 | 3.955374e-60 | 7.423547e-57 |
| MsG0680034445.01 | MsG0880045684.01 | 0.806521 | 7.900379e-50 | 4.297218e-47 |
| MsG0680034445.01 | MsG0880046517.01 | 0.807893 | 4.045385e-50 | 2.279993e-47 |
| MsG0680034445.01 | MsG0880046901.01 | 0.806892 | 6.595693e-50 | 3.621935e-47 |
| MsG0680034445.01 | MsG0880047212.01 | 0.803778 | 2.963335e-49 | 1.502211e-46 |
| MsG0680034445.01 | MsG0880047696.01 | 0.803503 | 3.378722e-49 | 1.700805e-46 |
| MsG0480018479.01 | MsG0680034445.01 | 0.822863 | 1.886446e-53 | 1.598200e-50 |
| MsG0480021195.01 | MsG0680034445.01 | 0.806807 | 6.876587e-50 | 3.767908e-47 |
| MsG0480021432.01 | MsG0680034445.01 | 0.808384 | 3.179583e-50 | 1.815551e-47 |
| MsG0480021706.01 | MsG0680034445.01 | 0.830599 | 2.681893e-55 | 2.838941e-52 |
| MsG0480021724.01 | MsG0680034445.01 | 0.805005 | 1.644965e-49 | 8.606368e-47 |
| MsG0480021735.01 | MsG0680034445.01 | 0.816145 | 6.432232e-52 | 4.521446e-49 |
| MsG0480022223.01 | MsG0680034445.01 | 0.803471 | 3.432070e-49 | 1.726144e-46 |
| MsG0480022514.01 | MsG0680034445.01 | 0.810627 | 1.049157e-50 | 6.356253e-48 |
| MsG0480022672.01 | MsG0680034445.01 | 0.857661 | 1.402466e-62 | 3.487608e-59 |
| MsG0480022696.01 | MsG0680034445.01 | 0.810361 | 1.197707e-50 | 7.204865e-48 |
| MsG0480022780.01 | MsG0680034445.01 | 0.808524 | 2.969378e-50 | 1.701674e-47 |
| MsG0480023221.01 | MsG0680034445.01 | 0.804059 | 2.590146e-49 | 1.322621e-46 |
| MsG0480023240.01 | MsG0680034445.01 | 0.801770 | 7.696984e-49 | 3.706660e-46 |
| MsG0480023384.01 | MsG0680034445.01 | 0.810547 | 1.092012e-50 | 6.601376e-48 |
| MsG0480023387.01 | MsG0680034445.01 | 0.831341 | 1.763422e-55 | 1.908106e-52 |
| MsG0580024339.01 | MsG0680034445.01 | 0.813357 | 2.666614e-51 | 1.737619e-48 |
| MsG0580024619.01 | MsG0680034445.01 | 0.856850 | 2.434795e-62 | 5.889760e-59 |
| MsG0580024727.01 | MsG0680034445.01 | 0.823475 | 1.358094e-53 | 1.170557e-50 |
| MsG0580025268.01 | MsG0680034445.01 | 0.830226 | 3.308309e-55 | 3.464477e-52 |
| MsG0580025903.01 | MsG0680034445.01 | 0.809369 | 1.958259e-50 | 1.147660e-47 |
| MsG0380016005.01 | MsG0680034445.01 | 0.829128 | 6.122563e-55 | 6.209439e-52 |
| MsG0380016636.01 | MsG0680034445.01 | 0.803746 | 3.008396e-49 | 1.523829e-46 |
| MsG0380016923.01 | MsG0680034445.01 | 0.800722 | 1.261413e-48 | 5.915800e-46 |
| MsG0380017797.01 | MsG0680034445.01 | 0.807887 | 4.057470e-50 | 2.286451e-47 |
| MsG0380018033.01 | MsG0680034445.01 | 0.849952 | 2.323588e-60 | 4.479746e-57 |
| MsG0580026137.01 | MsG0680034445.01 | 0.805023 | 1.630614e-49 | 8.535145e-47 |
| MsG0580026485.01 | MsG0680034445.01 | 0.824592 | 7.426876e-54 | 6.608346e-51 |
| MsG0580028094.01 | MsG0680034445.01 | 0.829225 | 5.797605e-55 | 5.897290e-52 |
| MsG0580028582.01 | MsG0680034445.01 | 0.816462 | 5.463009e-52 | 3.873278e-49 |
| MsG0580028597.01 | MsG0680034445.01 | 0.815686 | 8.139656e-52 | 5.650447e-49 |
| MsG0580029231.01 | MsG0680034445.01 | 0.807328 | 5.332782e-50 | 2.961852e-47 |
| MsG0580029647.01 | MsG0680034445.01 | 0.807101 | 5.956695e-50 | 3.288722e-47 |
| MsG0280007847.01 | MsG0680034445.01 | 0.819650 | 1.039242e-52 | 8.044575e-50 |
| MsG0280007992.01 | MsG0680034445.01 | 0.810915 | 9.088258e-51 | 5.548896e-48 |
| MsG0280009575.01 | MsG0680034445.01 | 0.824033 | 1.005060e-53 | 8.799934e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034445.01.T01 | MTR_6g068850 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 2.95e-78 | 225 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034445.01.T01 | AT5G51960 | 60.000 | 110 | 37 | 1 | 1 | 110 | 1 | 103 | 2.41e-42 | 134 |
| MsG0680034445.01.T01 | AT5G51960 | 60.000 | 110 | 37 | 1 | 1 | 110 | 1 | 103 | 2.41e-42 | 134 |
Find 19 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTATACTTATATGCTTAA+TGG | 0.190532 | 6:+87739049 | MsG0680034445.01.T01:CDS |
| AAGGCGGTCAAGAAACATAT+TGG | 0.364646 | 6:+87738917 | MsG0680034445.01.T01:CDS |
| GGCGATAGATGTTAACTGTT+TGG | 0.367879 | 6:-87738886 | None:intergenic |
| TCTTGACCGCCTTGAGAAGA+TGG | 0.432707 | 6:-87738907 | None:intergenic |
| AGGCGGTCAAGAAACATATT+GGG | 0.477390 | 6:+87738918 | MsG0680034445.01.T01:CDS |
| CCTCGGAGTTCCACAAGAAT+CGG | 0.490759 | 6:+87738978 | MsG0680034445.01.T01:CDS |
| ATAGATGTTAACTGTTTGGA+AGG | 0.518205 | 6:-87738882 | None:intergenic |
| CAGATAGATTCCGATTCTTG+TGG | 0.534816 | 6:-87738988 | None:intergenic |
| CATTATGATTGATAAACCTC+AGG | 0.553941 | 6:-87739571 | None:intergenic |
| TGTTGGTCTTCAGCTTCCTG+AGG | 0.560132 | 6:+87739555 | MsG0680034445.01.T01:CDS |
| GGAAAATCTGCTGCAAGTGT+TGG | 0.562393 | 6:+87739538 | MsG0680034445.01.T01:CDS |
| CATCTATCGCCATCTTCTCA+AGG | 0.575149 | 6:+87738898 | MsG0680034445.01.T01:CDS |
| AATGAAGTGAAAAGAACACT+TGG | 0.586563 | 6:+87739517 | MsG0680034445.01.T01:CDS |
| AACAAAGCAATACCTTGTGA+TGG | 0.597418 | 6:-87739078 | None:intergenic |
| AAGAATCGGAATCTATCTGA+TGG | 0.607686 | 6:+87738992 | MsG0680034445.01.T01:CDS |
| TAATGGTGTGCACCATCACA+AGG | 0.617495 | 6:+87739066 | MsG0680034445.01.T01:CDS |
| CCGATTCTTGTGGAACTCCG+AGG | 0.631297 | 6:-87738978 | None:intergenic |
| TCGGAATCTATCTGATGGTG+TGG | 0.677039 | 6:+87738997 | MsG0680034445.01.T01:CDS |
| CTATCGCCATCTTCTCAAGG+CGG | 0.739647 | 6:+87738901 | MsG0680034445.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTATACTTATATGCTTAA+TGG | + | Chr6:87739049-87739068 | MsG0680034445.01.T01:CDS | 20.0% |
| !! | TATTGCAAAATAAAAGTAAG+AGG | + | Chr6:87739233-87739252 | MsG0680034445.01.T01:intron | 20.0% |
| !!! | TTTTTTAGAATTTGTAACCT+CGG | + | Chr6:87738961-87738980 | MsG0680034445.01.T01:CDS | 20.0% |
| ! | AAAATCAGCCAAATAAAATG+TGG | - | Chr6:87739426-87739445 | None:intergenic | 25.0% |
| AATGAAGTGAAAAGAACACT+TGG | + | Chr6:87739517-87739536 | MsG0680034445.01.T01:CDS | 30.0% | |
| AGATTGTTGACAATGATTCA+TGG | - | Chr6:87739453-87739472 | None:intergenic | 30.0% | |
| ATAGATGTTAACTGTTTGGA+AGG | - | Chr6:87738885-87738904 | None:intergenic | 30.0% | |
| GTCTAGTGTAAATCATTACT+AGG | + | Chr6:87739394-87739413 | MsG0680034445.01.T01:intron | 30.0% | |
| GTGCAGATGTAGTATTATAA+TGG | + | Chr6:87739334-87739353 | MsG0680034445.01.T01:intron | 30.0% | |
| TTATAATACTACATCTGCAC+AGG | - | Chr6:87739334-87739353 | None:intergenic | 30.0% | |
| ! | GGAAAAAGCCACATTTTATT+TGG | + | Chr6:87739415-87739434 | MsG0680034445.01.T01:intron | 30.0% |
| AACAAAGCAATACCTTGTGA+TGG | - | Chr6:87739081-87739100 | None:intergenic | 35.0% | |
| AAGAATCGGAATCTATCTGA+TGG | + | Chr6:87738992-87739011 | MsG0680034445.01.T01:CDS | 35.0% | |
| AATACACGTGACTGTTTGAA+GGG | - | Chr6:87739117-87739136 | None:intergenic | 35.0% | |
| ATCATTGTCAACAATCTTGC+AGG | + | Chr6:87739456-87739475 | MsG0680034445.01.T01:intron | 35.0% | |
| ! | AAAAGTAAGAGGTGTAGAAG+AGG | + | Chr6:87739244-87739263 | MsG0680034445.01.T01:intron | 35.0% |
| !!! | GAGCAAGCTTTATTTTCTGT+TGG | - | Chr6:87739027-87739046 | None:intergenic | 35.0% |
| AAGGCGGTCAAGAAACATAT+TGG | + | Chr6:87738917-87738936 | MsG0680034445.01.T01:CDS | 40.0% | |
| AGCCTGTACATTGTTCTGAT+TGG | + | Chr6:87739141-87739160 | MsG0680034445.01.T01:intron | 40.0% | |
| AGGCGGTCAAGAAACATATT+GGG | + | Chr6:87738918-87738937 | MsG0680034445.01.T01:CDS | 40.0% | |
| ATACTACATCTGCACAGGAT+TGG | - | Chr6:87739329-87739348 | None:intergenic | 40.0% | |
| CAATACACGTGACTGTTTGA+AGG | - | Chr6:87739118-87739137 | None:intergenic | 40.0% | |
| CAGATAGATTCCGATTCTTG+TGG | - | Chr6:87738991-87739010 | None:intergenic | 40.0% | |
| GAAATTCAGCATAGCCAAGT+TGG | + | Chr6:87739296-87739315 | MsG0680034445.01.T01:intron | 40.0% | |
| GACCAATCAGAACAATGTAC+AGG | - | Chr6:87739146-87739165 | None:intergenic | 40.0% | |
| GGCGATAGATGTTAACTGTT+TGG | - | Chr6:87738889-87738908 | None:intergenic | 40.0% | |
| CATCTATCGCCATCTTCTCA+AGG | + | Chr6:87738898-87738917 | MsG0680034445.01.T01:CDS | 45.0% | |
| GGAAAATCTGCTGCAAGTGT+TGG | + | Chr6:87739538-87739557 | MsG0680034445.01.T01:CDS | 45.0% | |
| TAATGGTGTGCACCATCACA+AGG | + | Chr6:87739066-87739085 | MsG0680034445.01.T01:CDS | 45.0% | |
| ! | TCGGAATCTATCTGATGGTG+TGG | + | Chr6:87738997-87739016 | MsG0680034445.01.T01:CDS | 45.0% |
| !! | GGATTGGCTTGAATCCAACT+TGG | - | Chr6:87739313-87739332 | None:intergenic | 45.0% |
| CCTCGGAGTTCCACAAGAAT+CGG | + | Chr6:87738978-87738997 | MsG0680034445.01.T01:CDS | 50.0% | |
| CTATCGCCATCTTCTCAAGG+CGG | + | Chr6:87738901-87738920 | MsG0680034445.01.T01:CDS | 50.0% | |
| TCTTGACCGCCTTGAGAAGA+TGG | - | Chr6:87738910-87738929 | None:intergenic | 50.0% | |
| !! | TGTTGGTCTTCAGCTTCCTG+AGG | + | Chr6:87739555-87739574 | MsG0680034445.01.T01:CDS | 50.0% |
| CCGATTCTTGTGGAACTCCG+AGG | - | Chr6:87738981-87739000 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 87738869 | 87739591 | 87738869 | ID=MsG0680034445.01;Name=MsG0680034445.01 |
| Chr6 | mRNA | 87738869 | 87739591 | 87738869 | ID=MsG0680034445.01.T01;Parent=MsG0680034445.01;Name=MsG0680034445.01.T01;_AED=0.45;_eAED=0.46;_QI=0|0|0|1|1|1|2|0|110 |
| Chr6 | exon | 87738869 | 87739087 | 87738869 | ID=MsG0680034445.01.T01:exon:13441;Parent=MsG0680034445.01.T01 |
| Chr6 | exon | 87739478 | 87739591 | 87739478 | ID=MsG0680034445.01.T01:exon:13442;Parent=MsG0680034445.01.T01 |
| Chr6 | CDS | 87738869 | 87739087 | 87738869 | ID=MsG0680034445.01.T01:cds;Parent=MsG0680034445.01.T01 |
| Chr6 | CDS | 87739478 | 87739591 | 87739478 | ID=MsG0680034445.01.T01:cds;Parent=MsG0680034445.01.T01 |
| Gene Sequence |
| Protein sequence |
