AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007335.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007335.01.T01 MTR_8g064470 94.382 89 5 0 8 96 576 664 1.31e-45 155
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 13 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTTCTCCTCTCATCTTC+TGG 0.357567 2:-13838690 MsG0280007335.01.T01:CDS
GAATCTTCTCCGTCATCTGC+TGG 0.462652 2:-13838576 MsG0280007335.01.T01:CDS
GAATCTTCGCCGTCATCTGC+TGG 0.474227 2:-13838519 MsG0280007335.01.T01:CDS
ATCTTCGATCGAAGAGTCCT+TGG 0.482268 2:+13838470 None:intergenic
GAATCGTCTCTGTCATCTGC+TGG 0.498900 2:-13838633 MsG0280007335.01.T01:CDS
AAGGACTCTTCGATCGAAGA+TGG 0.544420 2:-13838468 MsG0280007335.01.T01:CDS
TGACAGAGACGATTCTGTCT+TGG 0.561155 2:+13838641 None:intergenic
TGACGGCGAAGATTCTGTCT+TGG 0.569351 2:+13838527 None:intergenic
TGACGGAGAAGATTCTGTCT+TGG 0.569351 2:+13838584 None:intergenic
ATTGCAGCGCCAGCAGATGA+CGG 0.580513 2:+13838567 None:intergenic
TTTGCAACACCAGCAGATGA+CGG 0.613033 2:+13838510 None:intergenic
TTGCAGCGCCAGAAGATGAG+AGG 0.615069 2:+13838682 None:intergenic
TGAATGTGTTGAGACTGCCA+AGG 0.781648 2:-13838487 MsG0280007335.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATCTTCTCCTCTCATCTTC+TGG - Chr2:13838446-13838465 MsG0280007335.01.T01:CDS 40.0%
AAGGACTCTTCGATCGAAGA+TGG - Chr2:13838668-13838687 MsG0280007335.01.T01:CDS 45.0%
ATCTTCGATCGAAGAGTCCT+TGG + Chr2:13838669-13838688 None:intergenic 45.0%
TGAATGTGTTGAGACTGCCA+AGG - Chr2:13838649-13838668 MsG0280007335.01.T01:CDS 45.0%
TGACAGAGACGATTCTGTCT+TGG + Chr2:13838498-13838517 None:intergenic 45.0%
TGACGGAGAAGATTCTGTCT+TGG + Chr2:13838555-13838574 None:intergenic 45.0%
TTTGCAACACCAGCAGATGA+CGG + Chr2:13838629-13838648 None:intergenic 45.0%
GAATCGTCTCTGTCATCTGC+TGG - Chr2:13838503-13838522 MsG0280007335.01.T01:CDS 50.0%
GAATCTTCTCCGTCATCTGC+TGG - Chr2:13838560-13838579 MsG0280007335.01.T01:CDS 50.0%
TGACGGCGAAGATTCTGTCT+TGG + Chr2:13838612-13838631 None:intergenic 50.0%
ATTGCAGCGCCAGCAGATGA+CGG + Chr2:13838572-13838591 None:intergenic 55.0%
GAATCTTCGCCGTCATCTGC+TGG - Chr2:13838617-13838636 MsG0280007335.01.T01:CDS 55.0%
TTGCAGCGCCAGAAGATGAG+AGG + Chr2:13838457-13838476 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 13838434 13838724 13838434 ID=MsG0280007335.01;Name=MsG0280007335.01
Chr2 mRNA 13838434 13838724 13838434 ID=MsG0280007335.01.T01;Parent=MsG0280007335.01;Name=MsG0280007335.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|96
Chr2 exon 13838434 13838724 13838434 ID=MsG0280007335.01.T01:exon:9427;Parent=MsG0280007335.01.T01
Chr2 CDS 13838434 13838724 13838434 ID=MsG0280007335.01.T01:cds;Parent=MsG0280007335.01.T01
Gene Sequence

>MsG0280007335.01.T01

ATGGCATCACAGAATCTTCTCCTCTCATCTTCTGGCGCTGCAACTGAATGCGTTGAGACTGCCAAGACAGAATCGTCTCTGTCATCTGCTGGCGCTGCAACTGAATGCGTTGAGACTGCCAAGACAGAATCTTCTCCGTCATCTGCTGGCGCTGCAATCGAATGTGTTGAGATTGCCAAGACAGAATCTTCGCCGTCATCTGCTGGTGTTGCAAATGAATGTGTTGAGACTGCCAAGGACTCTTCGATCGAAGATGGCATCACGAGTTGCAATTCTGACATTATTGAATGA

Protein sequence

>MsG0280007335.01.T01

MASQNLLLSSSGAATECVETAKTESSLSSAGAATECVETAKTESSPSSAGAAIECVEIAKTESSPSSAGVANECVETAKDSSIEDGITSCNSDIIE*