AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1388720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1388720 MtrunA17_Chr7g0273731 91.164 464 22 1 1 464 84 528 0.0 879
Msa1388720 MtrunA17_Chr7g0273791 80.603 464 71 1 1 464 1 445 0.0 788
Msa1388720 MtrunA17_Chr7g0273781 78.664 464 80 1 1 464 34 478 0.0 778
Msa1388720 MtrunA17_Chr5g0433641 74.839 465 97 2 1 464 1 446 0.0 730
Msa1388720 MtrunA17_Chr7g0273761 74.409 465 98 3 2 464 374 819 0.0 724
Msa1388720 MtrunA17_Chr7g0273761 74.000 300 77 1 166 464 32 331 8.58e-162 478
Msa1388720 MtrunA17_Chr7g0273721 73.333 465 103 3 2 464 3 448 0.0 719
Msa1388720 MtrunA17_Chr1g0165671 56.550 458 172 6 6 460 23 456 0.0 533
Msa1388720 MtrunA17_Chr1g0165661 52.183 458 194 6 6 460 5 440 1.23e-169 485
Msa1388720 MtrunA17_Chr1g0191081 53.879 464 190 5 3 464 11 452 2.98e-169 484
Msa1388720 MtrunA17_Chr1g0165681 51.965 458 195 6 6 460 5 440 1.52e-168 482
Msa1388720 MtrunA17_Chr1g0165711 47.712 459 175 7 6 463 5 399 3.20e-142 414
Msa1388720 MtrunA17_Chr7g0273741 82.993 147 25 0 1 147 1 147 1.77e-84 256
Msa1388720 MtrunA17_Chr6g0471381 54.435 248 105 5 217 460 6 249 3.54e-84 260
Msa1388720 MtrunA17_Chr7g0273701 72.500 160 44 0 305 464 1 160 8.96e-82 250
Msa1388720 MtrunA17_Chr3g0098961 76.871 147 34 0 1 147 4 150 8.21e-78 240
Msa1388720 MtrunA17_Chr3g0132001 32.143 448 266 10 10 452 14 428 2.19e-68 224
Msa1388720 MtrunA17_Chr8g0386141 32.353 408 242 10 8 409 5 384 7.36e-68 223
Msa1388720 MtrunA17_Chr7g0224391 31.514 403 246 7 13 411 19 395 1.88e-65 217
Msa1388720 MtrunA17_Chr6g0471971 46.667 240 96 6 181 414 1 214 2.20e-65 209
Msa1388720 MtrunA17_Chr6g0471371 63.087 149 55 0 6 154 5 153 3.15e-65 207
Msa1388720 MtrunA17_Chr1g0165741 63.380 142 52 0 6 147 5 146 2.03e-62 199
Msa1388720 MtrunA17_Chr8g0353421 62.992 127 47 0 3 129 2 128 1.28e-57 186
Msa1388720 MtrunA17_Chr3g0141851 30.170 411 241 12 18 415 20 397 2.30e-51 180
Msa1388720 MtrunA17_Chr2g0306321 29.158 463 285 15 8 456 24 457 3.22e-48 172
Msa1388720 MtrunA17_Chr7g0273691 74.227 97 24 1 185 280 1 97 5.35e-45 152
Msa1388720 MtrunA17_Chr4g0027071 27.897 466 280 15 17 457 19 453 4.05e-43 158
Msa1388720 MtrunA17_Chr6g0471891 44.444 198 81 5 251 445 1 172 1.79e-42 150
Msa1388720 MtrunA17_Chr2g0334001 27.511 458 288 13 6 452 1 425 3.34e-42 155
Msa1388720 MtrunA17_Chr4g0001861 27.945 433 250 12 42 452 34 426 1.43e-41 153
Msa1388720 MtrunA17_Chr3g0113821 27.802 464 285 14 9 455 4 434 2.32e-39 147
Msa1388720 MtrunA17_Chr7g0270771 28.115 313 174 7 6 310 1 270 5.63e-38 139
Msa1388720 MtrunA17_Chr8g0371821 26.407 462 292 14 6 456 1 425 7.40e-38 143
Msa1388720 MtrunA17_Chr2g0283361 27.252 444 272 13 31 453 25 438 1.41e-32 129
Msa1388720 MtrunA17_Chr7g0253501 27.536 483 265 21 17 454 9 451 3.81e-32 127
Msa1388720 MtrunA17_Chr7g0253531 26.752 471 268 19 17 447 9 442 1.72e-31 125
Msa1388720 MtrunA17_Chr4g0072041 25.223 448 274 14 36 454 39 454 1.26e-30 123
Msa1388720 MtrunA17_Chr3g0087401 25.000 428 261 12 18 413 10 409 2.09e-30 122
Msa1388720 MtrunA17_Chr7g0273681 92.308 52 4 0 92 143 1 52 3.49e-29 108
Msa1388720 MtrunA17_Chr3g0106691 27.251 422 265 13 4 408 52 448 4.10e-29 119
Msa1388720 MtrunA17_Chr1g0165721 57.292 96 40 1 366 460 1 96 1.17e-27 106
Msa1388720 MtrunA17_Chr7g0253541 25.407 492 275 22 18 460 12 460 1.33e-27 114
Msa1388720 MtrunA17_Chr6g0470591 25.822 426 261 16 60 459 66 462 1.52e-27 114
Msa1388720 MtrunA17_Chr4g0054081 23.778 450 279 14 36 454 34 450 2.80e-27 114
Msa1388720 MtrunA17_Chr2g0311401 28.000 300 197 8 166 454 6 297 3.64e-27 110
Msa1388720 MtrunA17_Chr7g0252731 25.537 419 254 14 3 409 8 380 1.80e-26 111
Msa1388720 MtrunA17_Chr1g0165731 42.727 110 59 3 181 288 1 108 4.86e-25 99.4
Msa1388720 MtrunA17_Chr5g0442691 23.093 472 294 16 8 444 31 468 6.75e-25 107
Msa1388720 MtrunA17_Chr5g0442701 23.517 472 292 16 8 444 8 445 3.59e-24 104
Msa1388720 MtrunA17_Chr3g0106711 31.081 222 122 6 4 217 6 204 1.32e-23 99.4
Msa1388720 MtrunA17_Chr3g0106661 25.988 481 285 17 17 459 17 464 6.11e-23 101
Msa1388720 MtrunA17_Chr3g0106721 26.184 359 221 10 17 358 17 348 2.09e-22 99.4
Msa1388720 MtrunA17_Chr5g0442721 22.912 419 258 12 62 447 64 450 3.23e-22 99.0
Msa1388720 MtrunA17_Chr8g0354371 22.796 465 272 17 24 459 31 437 2.11e-20 93.2
Msa1388720 MtrunA17_Chr3g0095731 23.009 452 280 16 43 460 43 460 8.31e-20 91.7
Msa1388720 MtrunA17_Chr3g0095721 23.239 426 264 13 65 460 72 464 4.96e-19 89.4
Msa1388720 MtrunA17_Chr7g0273671 88.372 43 5 0 1 43 1 43 6.45e-19 80.1
Msa1388720 MtrunA17_Chr3g0123061 22.101 457 283 18 16 457 11 409 8.35e-19 88.2
Msa1388720 MtrunA17_Chr4g0004691 28.108 185 108 3 20 204 14 173 2.08e-18 83.2
Msa1388720 MtrunA17_Chr3g0134091 26.724 464 261 22 24 458 30 443 3.45e-18 86.7
Msa1388720 MtrunA17_Chr3g0134121 26.667 450 262 22 25 449 31 437 6.25e-18 86.3
Msa1388720 MtrunA17_Chr3g0134061 26.464 427 250 19 37 438 42 429 8.10e-18 85.9
Msa1388720 MtrunA17_Chr3g0106761 25.613 367 227 13 60 408 9 347 9.09e-18 85.1
Msa1388720 MtrunA17_Chr4g0010331 23.438 448 282 17 24 455 25 427 3.73e-17 83.6
Msa1388720 MtrunA17_Chr6g0471981 64.706 51 18 0 101 151 1 51 4.40e-17 75.5
Msa1388720 MtrunA17_Chr3g0106741 29.297 256 164 9 166 408 127 378 1.34e-16 81.6
Msa1388720 MtrunA17_Chr8g0376911 22.222 486 264 21 16 457 9 424 2.47e-16 80.9
Msa1388720 MtrunA17_Chr4g0010361 25.721 416 245 20 24 416 25 399 5.16e-16 80.1
Msa1388720 MtrunA17_Chr4g0010311 24.508 457 272 20 24 455 25 433 6.73e-16 79.7
Msa1388720 MtrunA17_Chr8g0374641 22.010 418 257 15 24 418 30 401 1.08e-15 79.0
Msa1388720 MtrunA17_Chr1g0199561 22.197 437 280 15 16 427 9 410 2.51e-15 77.8
Msa1388720 MtrunA17_Chr8g0376901 23.256 473 274 19 16 456 9 424 6.30e-15 76.6
Msa1388720 MtrunA17_Chr5g0414431 55.357 56 20 1 52 107 2 52 4.24e-14 66.6
Msa1388720 MtrunA17_Chr7g0224131 22.819 447 289 15 24 455 25 430 5.22e-14 73.9
Msa1388720 MtrunA17_Chr4g0053111 21.861 462 282 17 24 455 28 440 1.23e-13 72.8
Msa1388720 MtrunA17_Chr4g0010351 25.392 319 189 15 24 328 25 308 4.29e-13 70.5
Msa1388720 MtrunA17_Chr3g0106631 28.571 140 95 3 167 302 2 140 2.27e-11 62.4
Msa1388720 MtrunA17_Chr2g0311411 31.532 111 72 3 6 116 1 107 7.26e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1388720 AT5G17540.1 47.210 466 217 9 3 463 2 443 1.68e-141 414
Msa1388720 AT3G03480.1 45.161 465 225 9 2 461 11 450 1.46e-135 398
Msa1388720 AT3G48720.1 32.353 408 246 10 8 411 7 388 1.60e-69 228
Msa1388720 AT5G41040.1 32.108 408 245 8 10 411 33 414 6.58e-65 216
Msa1388720 AT5G63560.1 33.907 407 233 10 10 411 9 384 7.61e-65 215
Msa1388720 AT5G41040.2 32.108 408 245 8 10 411 17 398 8.09e-65 216
Msa1388720 AT1G27620.1 30.751 413 243 12 13 415 12 391 6.58e-52 181
Msa1388720 AT1G03390.1 30.222 450 270 15 8 444 23 441 1.04e-47 171
Msa1388720 AT5G48930.1 27.451 459 282 14 17 459 10 433 2.18e-42 155
Msa1388720 AT2G19070.1 28.477 453 262 15 36 458 29 449 4.05e-41 152
Msa1388720 AT2G40230.1 29.024 410 247 12 17 412 12 391 8.29e-39 146
Msa1388720 AT3G62160.1 26.316 418 250 12 6 409 1 374 3.95e-37 141
Msa1388720 AT1G28680.1 26.697 442 286 11 31 459 27 443 2.32e-35 137
Msa1388720 AT5G57840.1 27.044 477 283 15 10 461 3 439 4.13e-35 136
Msa1388720 AT1G28680.2 28.653 349 217 9 31 372 27 350 3.52e-32 127
Msa1388720 AT1G65450.1 26.291 426 266 13 3 410 8 403 5.48e-31 124
Msa1388720 AT5G07080.1 25.527 427 267 11 3 408 7 403 1.70e-30 123
Msa1388720 AT3G47170.1 24.280 486 306 17 8 460 10 466 1.68e-28 117
Msa1388720 AT2G25150.1 27.934 426 253 17 5 408 7 400 2.42e-27 114
Msa1388720 AT2G23510.1 26.066 422 267 16 2 408 7 398 1.88e-25 108
Msa1388720 AT1G32910.1 24.103 390 236 12 63 428 63 416 2.77e-21 96.7
Msa1388720 AT3G50280.1 25.537 419 247 17 24 418 28 405 3.09e-20 93.2
Msa1388720 AT1G78990.1 22.779 439 280 12 43 460 45 445 1.87e-19 90.9
Msa1388720 AT3G26040.1 22.747 466 281 18 16 455 9 421 6.59e-19 89.4
Msa1388720 AT1G24430.1 23.218 435 261 15 44 460 41 420 1.55e-18 88.2
Msa1388720 AT4G31910.1 24.454 458 286 18 26 454 27 453 3.26e-18 87.4
Msa1388720 AT5G07850.1 25.732 478 278 20 14 459 17 449 1.12e-17 85.5
Msa1388720 AT5G07860.1 24.893 466 289 18 14 458 17 442 3.66e-17 84.0
Msa1388720 AT5G16410.1 22.283 368 229 12 65 411 91 422 4.77e-17 84.0
Msa1388720 AT3G50270.1 23.227 409 257 13 36 418 33 410 6.35e-16 80.1
Msa1388720 AT5G47980.1 24.569 464 278 21 14 452 9 425 5.34e-15 77.4
Msa1388720 AT1G24420.2 22.500 400 211 12 76 441 76 410 2.52e-14 75.1
Msa1388720 AT1G24420.1 22.500 400 211 12 76 441 76 410 2.52e-14 75.1
Msa1388720 AT5G07870.1 23.517 472 304 19 14 459 17 457 1.55e-12 69.7
Msa1388720 AT1G65450.3 23.729 295 200 9 179 460 3 285 2.68e-12 67.8
Msa1388720 AT5G47950.1 24.735 283 167 9 16 294 12 252 6.71e-12 67.8

Find 135 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATAAAGATTGATGTATT+TGG 0.213067 tig0021157:-112518 None:intergenic
GCATGTCTAAGAACTTTAAT+AGG 0.218647 tig0021157:-109519 None:intergenic
AGCAGATCTTATGGTTATTA+AGG 0.242062 tig0021157:+112076 Msa1388720:CDS
AAGTTCAAAGAAGTGGATTT+TGG 0.248604 tig0021157:+112155 Msa1388720:CDS
ATGTAACACTTGATCAATTT+GGG 0.256344 tig0021157:+109661 Msa1388720:CDS
ACTTGACAAGATTTAGTTAC+AGG 0.266265 tig0021157:-109000 None:intergenic
TGTAGTATCTTCTTCCTTGA+TGG 0.282783 tig0021157:-111694 None:intergenic
ATCCCACATAAGAATGTAAA+AGG 0.288527 tig0021157:+112248 Msa1388720:CDS
GATGTAACACTTGATCAATT+TGG 0.298925 tig0021157:+109660 Msa1388720:CDS
TGCTTGGCAACGACACAATA+AGG 0.312592 tig0021157:+109178 Msa1388720:five_prime_UTR
AAACTGGCACATGAAATTTA+AGG 0.316301 tig0021157:-109113 None:intergenic
ATCCATTTGCAGGAAGGATT+AGG 0.320371 tig0021157:+109568 Msa1388720:CDS
ATGAATATTATTGGGAAAAC+TGG 0.323391 tig0021157:-109129 None:intergenic
CCATTCAACCTGTATGGAAT+AGG 0.323735 tig0021157:+111595 Msa1388720:CDS
GCATTCTGTAGCAGATCTTA+TGG 0.324794 tig0021157:+112067 Msa1388720:CDS
TCCATTTGCAGGAAGGATTA+GGG 0.331515 tig0021157:+109569 Msa1388720:CDS
TCGCCACTTGTTGGATATTA+CGG 0.334686 tig0021157:+111927 Msa1388720:CDS
TTTGGGTGATGAATATTATT+GGG 0.337019 tig0021157:-109137 None:intergenic
CATAGCTGCGCTTCGTCTCT+TGG 0.337255 tig0021157:+111755 Msa1388720:CDS
AAGCTTTGTGGAAACTCATT+AGG 0.345233 tig0021157:+111990 Msa1388720:CDS
AACTAGGAACCATTTGCTTT+AGG 0.348486 tig0021157:-112365 None:intergenic
CCCGCGGTGGTGACAGCTGC+CGG 0.349688 tig0021157:-111966 None:intergenic
GCAGCTGGTGGCACCAGCTC+TGG 0.355758 tig0021157:-109381 None:intergenic
GTGTATTACTATCCATTTGC+AGG 0.355829 tig0021157:+109558 Msa1388720:CDS
CACAAAAGCATTGCAGTTAG+AGG 0.365161 tig0021157:+111842 Msa1388720:CDS
ATTCTTATGTGGGATGATAT+AGG 0.365851 tig0021157:-112240 None:intergenic
GGATGGGGTGAGGCGGTGTA+TGG 0.375232 tig0021157:+112176 Msa1388720:CDS
GGGTGCTGGGCGTAAGTTGA+TGG 0.381264 tig0021157:+109593 Msa1388720:CDS
CATGAATGCCTGGGCAGAAA+TGG 0.382624 tig0021157:+111551 Msa1388720:CDS
CTCCGCACTGTGAACAATAA+TGG 0.390252 tig0021157:-109351 None:intergenic
GGATCACAAATAATTATTTA+CGG 0.390810 tig0021157:-112572 None:intergenic
CATGTCTAAGAACTTTAATA+GGG 0.395837 tig0021157:-109518 None:intergenic
CATTCAACCTGTATGGAATA+GGG 0.408225 tig0021157:+111596 Msa1388720:CDS
TATTCATGAACACGTGGAAT+AGG 0.410021 tig0021157:-109062 None:intergenic
TCCCTAATCCTTCCTGCAAA+TGG 0.413944 tig0021157:-109570 None:intergenic
GTTCTTTGAAGCATGGGAAT+GGG 0.414516 tig0021157:-109698 None:intergenic
AACCTTCCATTCAACCTGTA+TGG 0.427456 tig0021157:+111589 Msa1388720:CDS
GGAAGGATTAGGGAGGGTGC+TGG 0.433324 tig0021157:+109579 Msa1388720:CDS
TGAAACTATTCATGAATGCC+TGG 0.438374 tig0021157:+111541 Msa1388720:CDS
TCCCTTCACCACACCGGTCA+AGG 0.439345 tig0021157:+111035 Msa1388720:CDS
GAACTTCTATACGATGTTCC+AGG 0.439564 tig0021157:+109717 Msa1388720:CDS
TTTACCTGTATGAGTCGAAT+GGG 0.448569 tig0021157:-109762 None:intergenic
CAGCTCCATCGCTCATTGTA+TGG 0.451440 tig0021157:-111515 None:intergenic
CAAAATTAACCTAAAGCAAA+TGG 0.452519 tig0021157:+112356 Msa1388720:exon
CCTATTCCATACAGGTTGAA+TGG 0.453015 tig0021157:-111595 None:intergenic
GTTTATATCACATGCTTACA+TGG 0.453078 tig0021157:+109209 Msa1388720:five_prime_UTR
ACCACCGCGGGGAAGCTTTG+TGG 0.458585 tig0021157:+111978 Msa1388720:CDS
CTGGTGGCACCAGCTCTGGT+TGG 0.466144 tig0021157:-109377 None:intergenic
TGACGATAGATAAACATCAT+AGG 0.470860 tig0021157:-109471 None:intergenic
AAGAATGTAAAAGGAGAAGA+AGG 0.472789 tig0021157:+112257 Msa1388720:CDS
GCCCTTGACCGGTGTGGTGA+AGG 0.473028 tig0021157:-111037 None:intergenic
TCCTCGAGCCATTTCTGCCC+AGG 0.474546 tig0021157:-111559 None:intergenic
AGAATCTCCCTATTCCATAC+AGG 0.480630 tig0021157:-111603 None:intergenic
TCAATAATTAGTTCTGATCC+TGG 0.483822 tig0021157:-109735 None:intergenic
TTCCATACAGGTTGAATGGA+AGG 0.484244 tig0021157:-111591 None:intergenic
GTCAACCATTCGCCACTTGT+TGG 0.484423 tig0021157:+111918 Msa1388720:CDS
AGTTCTTTGAAGCATGGGAA+TGG 0.487665 tig0021157:-109699 None:intergenic
ATATAGGCTGCTCCAGGAAA+AGG 0.487690 tig0021157:-112224 None:intergenic
TGGGGTGAGGCGGTGTATGG+TGG 0.491453 tig0021157:+112179 Msa1388720:CDS
GCGGAGGAGCCAACCAGAGC+TGG 0.493190 tig0021157:+109368 Msa1388720:CDS
CCGCCCCATTCGACTCATAC+AGG 0.494546 tig0021157:+109758 Msa1388720:CDS
AAATATGTTCGTATTCATGA+TGG 0.495591 tig0021157:-111659 None:intergenic
CCCTTGACCGGTGTGGTGAA+GGG 0.498111 tig0021157:-111036 None:intergenic
CCGGCAGCTGTCACCACCGC+GGG 0.498263 tig0021157:+111966 Msa1388720:CDS
ATGGAATAGGGAGATTCTAA+TGG 0.500842 tig0021157:+111608 Msa1388720:CDS
ATACAGGAATTGAAAAGATA+AGG 0.505757 tig0021157:-109233 None:intergenic
TCGTATAAAGTTCAAAGAAG+TGG 0.508593 tig0021157:+112148 Msa1388720:CDS
TAGTTTAACTTCACGAGGAG+TGG 0.511679 tig0021157:-109406 None:intergenic
TATAGAAGTTCTTTGAAGCA+TGG 0.512144 tig0021157:-109705 None:intergenic
GAAGGATTAGGGAGGGTGCT+GGG 0.520089 tig0021157:+109580 Msa1388720:CDS
TTCTTTGAAGCATGGGAATG+GGG 0.526135 tig0021157:-109697 None:intergenic
ATGATAAACATGACAAAACT+AGG 0.530951 tig0021157:-112381 None:intergenic
CAAGGGCCTCATCGTAAGAA+TGG 0.533526 tig0021157:+111053 Msa1388720:CDS
CCGCGGTGGTGACAGCTGCC+GGG 0.534362 tig0021157:-111965 None:intergenic
ATAGAAGTTCTTTGAAGCAT+GGG 0.535113 tig0021157:-109704 None:intergenic
CCCTTCACCACACCGGTCAA+GGG 0.541833 tig0021157:+111036 Msa1388720:CDS
AAACCTCTTCATATCTTCAA+AGG 0.555864 tig0021157:-112303 None:intergenic
ATGGTGGATTGTACTGGTGA+GGG 0.557820 tig0021157:+109612 Msa1388720:CDS
GAAACTATTCATGAATGCCT+GGG 0.560588 tig0021157:+111542 Msa1388720:CDS
ATTACTATCCATTTGCAGGA+AGG 0.563934 tig0021157:+109562 Msa1388720:CDS
GGACAGAAGTCTAACTTGAA+AGG 0.564133 tig0021157:-109041 None:intergenic
ATCTTATGGTTATTAAGGAA+CGG 0.564453 tig0021157:+112081 Msa1388720:CDS
ATTAGGTTATGCTGTGGAGT+TGG 0.566011 tig0021157:+112007 Msa1388720:CDS
AGAAGAAAGATTGATGAACC+AGG 0.568559 tig0021157:-111719 None:intergenic
GGTTGCATGTAATGTGTGGT+GGG 0.570054 tig0021157:-111638 None:intergenic
GATGGTGGATTGTACTGGTG+AGG 0.570539 tig0021157:+109611 Msa1388720:CDS
TCACGAGGAGTGGAAGCAGC+TGG 0.571211 tig0021157:-109396 None:intergenic
AACTTGAAAGGATTTAGTAT+CGG 0.571421 tig0021157:-109029 None:intergenic
GGTGTTTATTTACAACTACA+AGG 0.572054 tig0021157:+112102 Msa1388720:CDS
TCCACAAAGCTTCCCCGCGG+TGG 0.581894 tig0021157:-111979 None:intergenic
CTTACGATGAGGCCCTTGAC+CGG 0.583352 tig0021157:-111048 None:intergenic
AAGTTGATGGTGGATTGTAC+TGG 0.588782 tig0021157:+109606 Msa1388720:CDS
TGTCCTTTGAAGATATGAAG+AGG 0.589462 tig0021157:+112300 Msa1388720:CDS
TGGTTGCATGTAATGTGTGG+TGG 0.590063 tig0021157:-111639 None:intergenic
AAAGAGTTAGATGAGATGCT+TGG 0.590438 tig0021157:+112329 Msa1388720:CDS
GGGATGATATAGGCTGCTCC+AGG 0.591783 tig0021157:-112230 None:intergenic
GGAAGAAGATACTACAACCC+TGG 0.593991 tig0021157:+111701 Msa1388720:CDS
GCATGAAACCACCGCACTTG+AGG 0.601841 tig0021157:-111476 None:intergenic
TTGAACCATACAATGAGCGA+TGG 0.603418 tig0021157:+111510 Msa1388720:CDS
GTACAATGACGAAGGTGAGA+TGG 0.604232 tig0021157:-111780 None:intergenic
TGCTGGGCGTAAGTTGATGG+TGG 0.605454 tig0021157:+109596 Msa1388720:CDS
CATACAATGAGCGATGGAGC+TGG 0.610758 tig0021157:+111516 Msa1388720:CDS
TCTTTGAAGCATGGGAATGG+GGG 0.613320 tig0021157:-109696 None:intergenic
GAAGAAAGATTGATGAACCA+GGG 0.616617 tig0021157:-111718 None:intergenic
AATATCCAACAAGTGGCGAA+TGG 0.616842 tig0021157:-111923 None:intergenic
ATCCATGTAAAATAAAGTTG+TGG 0.619116 tig0021157:+112591 Msa1388720:three_prime_UTR
TTACCGTAATATCCAACAAG+TGG 0.632459 tig0021157:-111930 None:intergenic
ATTAAAAGCTGAAGTTACAG+AGG 0.635766 tig0021157:+112037 Msa1388720:CDS
TGTAACACTTGATCAATTTG+GGG 0.637489 tig0021157:+109662 Msa1388720:CDS
TCTGACATAGATGATCAAGA+AGG 0.638298 tig0021157:+109432 Msa1388720:CDS
CGAGGAGTGGAAGCAGCTGG+TGG 0.639794 tig0021157:-109393 None:intergenic
TTGACCGGTGTGGTGAAGGG+AGG 0.647402 tig0021157:-111033 None:intergenic
GTTGAACCATTCTTACGATG+AGG 0.647528 tig0021157:-111059 None:intergenic
CATTATTGTTCACAGTGCGG+AGG 0.651919 tig0021157:+109352 Msa1388720:CDS
GCCTGGGCAGAAATGGCTCG+AGG 0.654434 tig0021157:+111558 Msa1388720:CDS
GCATCGTCAATGCACGCTCG+AGG 0.662333 tig0021157:+111889 Msa1388720:CDS
TCTACCTCCCTTCACCACAC+CGG 0.662546 tig0021157:+111029 Msa1388720:CDS
TTTGCAGGAAGGATTAGGGA+GGG 0.663911 tig0021157:+109573 Msa1388720:CDS
AAACTCATTAGGTTATGCTG+TGG 0.666130 tig0021157:+112001 Msa1388720:CDS
GATAATAGTTTAACTTCACG+AGG 0.669478 tig0021157:-109411 None:intergenic
TTACCTGTATGAGTCGAATG+GGG 0.671022 tig0021157:-109761 None:intergenic
CACCATTATTGTTCACAGTG+CGG 0.673411 tig0021157:+109349 Msa1388720:CDS
ATTTGCAGGAAGGATTAGGG+AGG 0.677174 tig0021157:+109572 Msa1388720:CDS
TGATGGTTGCATGTAATGTG+TGG 0.678806 tig0021157:-111642 None:intergenic
ATTACGTATTCATGAACACG+TGG 0.680316 tig0021157:-109068 None:intergenic
GTGACACGCCTCAAGTGCGG+TGG 0.681359 tig0021157:+111468 Msa1388720:CDS
CCTGTATGAGTCGAATGGGG+CGG 0.682735 tig0021157:-109758 None:intergenic
TAGGTGACACGCCTCAAGTG+CGG 0.682839 tig0021157:+111465 Msa1388720:intron
TCGTAAGAATGGTTCAACGA+GGG 0.694699 tig0021157:+111064 Msa1388720:CDS
CGAATGTCGTACAATGACGA+AGG 0.701737 tig0021157:-111788 None:intergenic
GATGAGGCCCTTGACCGGTG+TGG 0.732876 tig0021157:-111043 None:intergenic
GAATTGTGTACGTAAACCTG+TGG 0.747356 tig0021157:-109267 None:intergenic
CGGCAGCTGTCACCACCGCG+GGG 0.749713 tig0021157:+111967 Msa1388720:CDS
ATCGTAAGAATGGTTCAACG+AGG 0.760120 tig0021157:+111063 Msa1388720:CDS
GTTTCCACAAAGCTTCCCCG+CGG 0.764073 tig0021157:-111982 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATTATAAATTAAAAAA+TGG + tig0021157:110626-110645 Msa1388720:intron 0.0%
!!! ACATTTTAAGATATTATATT+TGG - tig0021157:110423-110442 None:intergenic 10.0%
!! ACTATATATAAAAAACATAA+GGG - tig0021157:111392-111411 None:intergenic 10.0%
!! TACTATATATAAAAAACATA+AGG - tig0021157:111393-111412 None:intergenic 10.0%
!!! AATCGCATTTTTTATTTTTT+GGG - tig0021157:109157-109176 None:intergenic 15.0%
!!! AAATCGCATTTTTTATTTTT+TGG - tig0021157:109158-109177 None:intergenic 15.0%
!!! TTTTTTTTATAATATGTTCC+AGG + tig0021157:109857-109876 Msa1388720:intron 15.0%
!! TAAACCCTATTTAAAAAAAT+TGG + tig0021157:110395-110414 Msa1388720:intron 15.0%
!! ATGTATAAGTGATTTAAATT+TGG + tig0021157:110439-110458 Msa1388720:intron 15.0%
!! TGTATAAGTGATTTAAATTT+GGG + tig0021157:110440-110459 Msa1388720:intron 15.0%
!! AAAAGTTATGAAAATTAACA+TGG + tig0021157:110510-110529 Msa1388720:intron 15.0%
!! AAAGTTATGAAAATTAACAT+GGG + tig0021157:110511-110530 Msa1388720:intron 15.0%
!!! CTTTTTTTATTTTAGATTAG+AGG - tig0021157:110886-110905 None:intergenic 15.0%
!!! CTCTAATCTAAAATAAAAAA+AGG + tig0021157:110884-110903 Msa1388720:intron 15.0%
!! TAATACATGTAAATTATGAA+CGG + tig0021157:111161-111180 Msa1388720:intron 15.0%
!!! TATTATATTTGCTATATTGT+TGG - tig0021157:111288-111307 None:intergenic 15.0%
!! TATAACATATATCTTGATAT+TGG + tig0021157:111429-111448 Msa1388720:intron 15.0%
!!! TAAACTTTTACTTAAATTTG+AGG + tig0021157:112542-112561 Msa1388720:three_prime_UTR 15.0%
!!! ATTGGAATATACGATTTTTT+TGG - tig0021157:110176-110195 None:intergenic 20.0%
!! ATTCCATTTCAAAAAATTTG+TGG - tig0021157:110347-110366 None:intergenic 20.0%
!!! GGATCCAATTTTTTTAAATA+GGG - tig0021157:110402-110421 None:intergenic 20.0%
!!! TGGATCCAATTTTTTTAAAT+AGG - tig0021157:110403-110422 None:intergenic 20.0%
!! GTATAAGTGATTTAAATTTG+GGG + tig0021157:110441-110460 Msa1388720:intron 20.0%
!!! GTTTATGCAATTTTTTTTAG+AGG + tig0021157:110687-110706 Msa1388720:intron 20.0%
!!! ATTTTTTTTAGAGGATGTTT+AGG + tig0021157:110696-110715 Msa1388720:intron 20.0%
!!! TGATTTTCATGTAAAAAAGA+CGG + tig0021157:111101-111120 Msa1388720:intron 20.0%
!!! AAATTTGTTCTGTTTATAAC+TGG + tig0021157:111353-111372 Msa1388720:intron 20.0%
!!! TAAAAAACATAAGGGATTTT+GGG - tig0021157:111384-111403 None:intergenic 20.0%
!!! ATAAAAAACATAAGGGATTT+TGG - tig0021157:111385-111404 None:intergenic 20.0%
!! TATTGGATTCAAAATATGAT+AGG + tig0021157:111446-111465 Msa1388720:intron 20.0%
!! TCTATAAAGATTGATGTATT+TGG - tig0021157:112521-112540 None:intergenic 20.0%
!! GGATCACAAATAATTATTTA+CGG - tig0021157:112575-112594 None:intergenic 20.0%
! AACTTGAAAGGATTTAGTAT+CGG - tig0021157:109032-109051 None:intergenic 25.0%
! ATGAATATTATTGGGAAAAC+TGG - tig0021157:109132-109151 None:intergenic 25.0%
! TTTGGGTGATGAATATTATT+GGG - tig0021157:109140-109159 None:intergenic 25.0%
!!! TTTTGGGTGATGAATATTAT+TGG - tig0021157:109141-109160 None:intergenic 25.0%
!! ATAAAAAATGCGATTTTGCT+TGG + tig0021157:109162-109181 Msa1388720:five_prime_UTR 25.0%
! ATACAGGAATTGAAAAGATA+AGG - tig0021157:109236-109255 None:intergenic 25.0%
!!! TTTCAATTCCTGTATTTTTG+TGG + tig0021157:109241-109260 Msa1388720:five_prime_UTR 25.0%
! CATGTCTAAGAACTTTAATA+GGG - tig0021157:109521-109540 None:intergenic 25.0%
! ATGTAACACTTGATCAATTT+GGG + tig0021157:109661-109680 Msa1388720:CDS 25.0%
! AGTGAGTATGTCAAATTTAT+TGG - tig0021157:109836-109855 None:intergenic 25.0%
! CATCTGTAACAACATAAAAT+TGG - tig0021157:110194-110213 None:intergenic 25.0%
!! CAAAATCACATCAATTTTCT+AGG + tig0021157:110765-110784 Msa1388720:intron 25.0%
! TATTAGCGCAATAAAAAGAA+CGG - tig0021157:111146-111165 None:intergenic 25.0%
! AAATATGTTCGTATTCATGA+TGG - tig0021157:111662-111681 None:intergenic 25.0%
!! ATCTTATCACTTCATGTTTT+TGG + tig0021157:111811-111830 Msa1388720:CDS 25.0%
! ATCTTATGGTTATTAAGGAA+CGG + tig0021157:112081-112100 Msa1388720:CDS 25.0%
! CAAAATTAACCTAAAGCAAA+TGG + tig0021157:112356-112375 Msa1388720:exon 25.0%
! ATGATAAACATGACAAAACT+AGG - tig0021157:112384-112403 None:intergenic 25.0%
! ATCCATGTAAAATAAAGTTG+TGG + tig0021157:112591-112610 Msa1388720:three_prime_UTR 25.0%
ACTTGACAAGATTTAGTTAC+AGG - tig0021157:109003-109022 None:intergenic 30.0%
AAACTGGCACATGAAATTTA+AGG - tig0021157:109116-109135 None:intergenic 30.0%
GTTTATATCACATGCTTACA+TGG + tig0021157:109209-109228 Msa1388720:five_prime_UTR 30.0%
! GATAATAGTTTAACTTCACG+AGG - tig0021157:109414-109433 None:intergenic 30.0%
TGACGATAGATAAACATCAT+AGG - tig0021157:109474-109493 None:intergenic 30.0%
!!! GGGTTTTTTTCTTTCATTGA+CGG - tig0021157:109501-109520 None:intergenic 30.0%
GCATGTCTAAGAACTTTAAT+AGG - tig0021157:109522-109541 None:intergenic 30.0%
GATGTAACACTTGATCAATT+TGG + tig0021157:109660-109679 Msa1388720:CDS 30.0%
TGTAACACTTGATCAATTTG+GGG + tig0021157:109662-109681 Msa1388720:CDS 30.0%
!! ATAGAAGTTCTTTGAAGCAT+GGG - tig0021157:109707-109726 None:intergenic 30.0%
!! TATAGAAGTTCTTTGAAGCA+TGG - tig0021157:109708-109727 None:intergenic 30.0%
!! TCAATAATTAGTTCTGATCC+TGG - tig0021157:109738-109757 None:intergenic 30.0%
TATGTAATTAGTTCAGATCC+TGG - tig0021157:109878-109897 None:intergenic 30.0%
TTTATGTTGTTACAGATGCA+TGG + tig0021157:110196-110215 Msa1388720:intron 30.0%
! GTCATCTTTTGTTAAAGGTT+TGG - tig0021157:110232-110251 None:intergenic 30.0%
! TAGAGGTCATCTTTTGTTAA+AGG - tig0021157:110237-110256 None:intergenic 30.0%
ATCTTTAAGCAACTCTTTAG+AGG - tig0021157:110254-110273 None:intergenic 30.0%
!!! GCTCCACAAATTTTTTGAAA+TGG + tig0021157:110341-110360 Msa1388720:intron 30.0%
GTCATCGTAAAAGATAAACA+TGG - tig0021157:111264-111283 None:intergenic 30.0%
! TTTTTCGTCAAATACCATCA+AGG + tig0021157:111680-111699 Msa1388720:CDS 30.0%
ATTAAAAGCTGAAGTTACAG+AGG + tig0021157:112037-112056 Msa1388720:CDS 30.0%
AGCAGATCTTATGGTTATTA+AGG + tig0021157:112076-112095 Msa1388720:CDS 30.0%
GGTGTTTATTTACAACTACA+AGG + tig0021157:112102-112121 Msa1388720:CDS 30.0%
TCGTATAAAGTTCAAAGAAG+TGG + tig0021157:112148-112167 Msa1388720:CDS 30.0%
!! AAGTTCAAAGAAGTGGATTT+TGG + tig0021157:112155-112174 Msa1388720:CDS 30.0%
ATTCTTATGTGGGATGATAT+AGG - tig0021157:112243-112262 None:intergenic 30.0%
ATCCCACATAAGAATGTAAA+AGG + tig0021157:112248-112267 Msa1388720:CDS 30.0%
! CTCCTTTTACATTCTTATGT+GGG - tig0021157:112253-112272 None:intergenic 30.0%
! TCTCCTTTTACATTCTTATG+TGG - tig0021157:112254-112273 None:intergenic 30.0%
AAGAATGTAAAAGGAGAAGA+AGG + tig0021157:112257-112276 Msa1388720:CDS 30.0%
AAACCTCTTCATATCTTCAA+AGG - tig0021157:112306-112325 None:intergenic 30.0%
!! CTTTTACTTAAATTTGAGGC+TGG + tig0021157:112546-112565 Msa1388720:three_prime_UTR 30.0%
!!! GTCCACAACTTTATTTTACA+TGG - tig0021157:112596-112615 None:intergenic 30.0%
TATTCATGAACACGTGGAAT+AGG - tig0021157:109065-109084 None:intergenic 35.0%
ATTACGTATTCATGAACACG+TGG - tig0021157:109071-109090 None:intergenic 35.0%
TCTGACATAGATGATCAAGA+AGG + tig0021157:109432-109451 Msa1388720:CDS 35.0%
GTGTATTACTATCCATTTGC+AGG + tig0021157:109558-109577 Msa1388720:CDS 35.0%
ATTACTATCCATTTGCAGGA+AGG + tig0021157:109562-109581 Msa1388720:CDS 35.0%
TTTACCTGTATGAGTCGAAT+GGG - tig0021157:109765-109784 None:intergenic 35.0%
! TTTTACCTGTATGAGTCGAA+TGG - tig0021157:109766-109785 None:intergenic 35.0%
GAATGCGATAATTGAAACGT+AGG - tig0021157:109814-109833 None:intergenic 35.0%
ACATGAGAAATCAACATGCA+TGG + tig0021157:110828-110847 Msa1388720:intron 35.0%
TGAAACTATTCATGAATGCC+TGG + tig0021157:111541-111560 Msa1388720:CDS 35.0%
GAAACTATTCATGAATGCCT+GGG + tig0021157:111542-111561 Msa1388720:CDS 35.0%
CATTCAACCTGTATGGAATA+GGG + tig0021157:111596-111615 Msa1388720:CDS 35.0%
ATGGAATAGGGAGATTCTAA+TGG + tig0021157:111608-111627 Msa1388720:CDS 35.0%
TGTAGTATCTTCTTCCTTGA+TGG - tig0021157:111697-111716 None:intergenic 35.0%
GAAGAAAGATTGATGAACCA+GGG - tig0021157:111721-111740 None:intergenic 35.0%
AGAAGAAAGATTGATGAACC+AGG - tig0021157:111722-111741 None:intergenic 35.0%
TTACCGTAATATCCAACAAG+TGG - tig0021157:111933-111952 None:intergenic 35.0%
! AAGCTTTGTGGAAACTCATT+AGG + tig0021157:111990-112009 Msa1388720:CDS 35.0%
AAACTCATTAGGTTATGCTG+TGG + tig0021157:112001-112020 Msa1388720:CDS 35.0%
!! TCAAAGAAGTGGATTTTGGA+TGG + tig0021157:112159-112178 Msa1388720:CDS 35.0%
!! CAAAGAAGTGGATTTTGGAT+GGG + tig0021157:112160-112179 Msa1388720:CDS 35.0%
!! AAAGAAGTGGATTTTGGATG+GGG + tig0021157:112161-112180 Msa1388720:CDS 35.0%
! TGTCCTTTGAAGATATGAAG+AGG + tig0021157:112300-112319 Msa1388720:CDS 35.0%
AAAGAGTTAGATGAGATGCT+TGG + tig0021157:112329-112348 Msa1388720:CDS 35.0%
AACTAGGAACCATTTGCTTT+AGG - tig0021157:112368-112387 None:intergenic 35.0%
GGACAGAAGTCTAACTTGAA+AGG - tig0021157:109044-109063 None:intergenic 40.0%
TGTGGTGACCACAAAAATAC+AGG - tig0021157:109252-109271 None:intergenic 40.0%
!! TGTATTTTTGTGGTCACCAC+AGG + tig0021157:109251-109270 Msa1388720:five_prime_UTR 40.0%
GAATTGTGTACGTAAACCTG+TGG - tig0021157:109270-109289 None:intergenic 40.0%
CACCATTATTGTTCACAGTG+CGG + tig0021157:109349-109368 Msa1388720:CDS 40.0%
! TAGTTTAACTTCACGAGGAG+TGG - tig0021157:109409-109428 None:intergenic 40.0%
ATCCATTTGCAGGAAGGATT+AGG + tig0021157:109568-109587 Msa1388720:CDS 40.0%
TCCATTTGCAGGAAGGATTA+GGG + tig0021157:109569-109588 Msa1388720:CDS 40.0%
!! AAGTTGATGGTGGATTGTAC+TGG + tig0021157:109606-109625 Msa1388720:CDS 40.0%
!! TTCTTTGAAGCATGGGAATG+GGG - tig0021157:109700-109719 None:intergenic 40.0%
!! GTTCTTTGAAGCATGGGAAT+GGG - tig0021157:109701-109720 None:intergenic 40.0%
!! AGTTCTTTGAAGCATGGGAA+TGG - tig0021157:109702-109721 None:intergenic 40.0%
GAACTTCTATACGATGTTCC+AGG + tig0021157:109717-109736 Msa1388720:CDS 40.0%
TTACCTGTATGAGTCGAATG+GGG - tig0021157:109764-109783 None:intergenic 40.0%
! ATGAGTGTACGCCTTTTAAG+TGG + tig0021157:110296-110315 Msa1388720:intron 40.0%
GTTGAACCATTCTTACGATG+AGG - tig0021157:111062-111081 None:intergenic 40.0%
ATCGTAAGAATGGTTCAACG+AGG + tig0021157:111063-111082 Msa1388720:CDS 40.0%
TCGTAAGAATGGTTCAACGA+GGG + tig0021157:111064-111083 Msa1388720:CDS 40.0%
!! TCAACGAGGGTATTCAGATA+AGG + tig0021157:111077-111096 Msa1388720:intron 40.0%
TTGAACCATACAATGAGCGA+TGG + tig0021157:111510-111529 Msa1388720:CDS 40.0%
AACCTTCCATTCAACCTGTA+TGG + tig0021157:111589-111608 Msa1388720:CDS 40.0%
! TTCCATACAGGTTGAATGGA+AGG - tig0021157:111594-111613 None:intergenic 40.0%
! CCTATTCCATACAGGTTGAA+TGG - tig0021157:111598-111617 None:intergenic 40.0%
CCATTCAACCTGTATGGAAT+AGG + tig0021157:111595-111614 Msa1388720:CDS 40.0%
AGAATCTCCCTATTCCATAC+AGG - tig0021157:111606-111625 None:intergenic 40.0%
TGATGGTTGCATGTAATGTG+TGG - tig0021157:111645-111664 None:intergenic 40.0%
! CTAACTGCAATGCTTTTGTG+CGG - tig0021157:111842-111861 None:intergenic 40.0%
!! CACAAAAGCATTGCAGTTAG+AGG + tig0021157:111842-111861 Msa1388720:CDS 40.0%
AATATCCAACAAGTGGCGAA+TGG - tig0021157:111926-111945 None:intergenic 40.0%
! TCGCCACTTGTTGGATATTA+CGG + tig0021157:111927-111946 Msa1388720:CDS 40.0%
!! CGGTAATTGTTTTGCTTACC+CGG + tig0021157:111947-111966 Msa1388720:CDS 40.0%
ATTAGGTTATGCTGTGGAGT+TGG + tig0021157:112007-112026 Msa1388720:CDS 40.0%
GCATTCTGTAGCAGATCTTA+TGG + tig0021157:112067-112086 Msa1388720:CDS 40.0%
TGCTTGGCAACGACACAATA+AGG + tig0021157:109178-109197 Msa1388720:five_prime_UTR 45.0%
CTCCGCACTGTGAACAATAA+TGG - tig0021157:109354-109373 None:intergenic 45.0%
CATTATTGTTCACAGTGCGG+AGG + tig0021157:109352-109371 Msa1388720:CDS 45.0%
TCCCTAATCCTTCCTGCAAA+TGG - tig0021157:109573-109592 None:intergenic 45.0%
ATTTGCAGGAAGGATTAGGG+AGG + tig0021157:109572-109591 Msa1388720:CDS 45.0%
TTTGCAGGAAGGATTAGGGA+GGG + tig0021157:109573-109592 Msa1388720:CDS 45.0%
!! ATGGTGGATTGTACTGGTGA+GGG + tig0021157:109612-109631 Msa1388720:CDS 45.0%
!! TCTTTGAAGCATGGGAATGG+GGG - tig0021157:109699-109718 None:intergenic 45.0%
!! AGTGTACGCCTTTTAAGTGG+CGG + tig0021157:110299-110318 Msa1388720:intron 45.0%
GGTTGCATGTAATGTGTGGT+GGG - tig0021157:111641-111660 None:intergenic 45.0%
TGGTTGCATGTAATGTGTGG+TGG - tig0021157:111642-111661 None:intergenic 45.0%
GGAAGAAGATACTACAACCC+TGG + tig0021157:111701-111720 Msa1388720:CDS 45.0%
GTACAATGACGAAGGTGAGA+TGG - tig0021157:111783-111802 None:intergenic 45.0%
CGAATGTCGTACAATGACGA+AGG - tig0021157:111791-111810 None:intergenic 45.0%
! TATGGTGGAGCTGCAAAAAG+TGG + tig0021157:112194-112213 Msa1388720:CDS 45.0%
! ATGGTGGAGCTGCAAAAAGT+GGG + tig0021157:112195-112214 Msa1388720:CDS 45.0%
ATATAGGCTGCTCCAGGAAA+AGG - tig0021157:112227-112246 None:intergenic 45.0%
!!! TTTTATATTTATTATATTGT+TGG - tig0021157:110063-110082 None:intergenic 5.0%
!! GATGGTGGATTGTACTGGTG+AGG + tig0021157:109611-109630 Msa1388720:CDS 50.0%
CAAGGGCCTCATCGTAAGAA+TGG + tig0021157:111053-111072 Msa1388720:CDS 50.0%
CAGCTCCATCGCTCATTGTA+TGG - tig0021157:111518-111537 None:intergenic 50.0%
CATACAATGAGCGATGGAGC+TGG + tig0021157:111516-111535 Msa1388720:CDS 50.0%
CATGAATGCCTGGGCAGAAA+TGG + tig0021157:111551-111570 Msa1388720:CDS 50.0%
GTCAACCATTCGCCACTTGT+TGG + tig0021157:111918-111937 Msa1388720:CDS 50.0%
!! AGTGGATTTTGGATGGGGTG+AGG + tig0021157:112166-112185 Msa1388720:CDS 50.0%
TGGTGGAGCTGCAAAAAGTG+GGG + tig0021157:112196-112215 Msa1388720:CDS 50.0%
!! GAAGGATTAGGGAGGGTGCT+GGG + tig0021157:109580-109599 Msa1388720:CDS 55.0%
!! TGCTGGGCGTAAGTTGATGG+TGG + tig0021157:109596-109615 Msa1388720:CDS 55.0%
CCTGTATGAGTCGAATGGGG+CGG - tig0021157:109761-109780 None:intergenic 55.0%
GTGTGGCTCCGCCACTTAAA+AGG - tig0021157:110310-110329 None:intergenic 55.0%
CGGAGCCACACACAACATGA+GGG + tig0021157:110319-110338 Msa1388720:intron 55.0%
TGGAGCCCTCATGTTGTGTG+TGG - tig0021157:110327-110346 None:intergenic 55.0%
TCTACCTCCCTTCACCACAC+CGG + tig0021157:111029-111048 Msa1388720:CDS 55.0%
CTTACGATGAGGCCCTTGAC+CGG - tig0021157:111051-111070 None:intergenic 55.0%
TAGGTGACACGCCTCAAGTG+CGG + tig0021157:111465-111484 Msa1388720:intron 55.0%
GCATGAAACCACCGCACTTG+AGG - tig0021157:111479-111498 None:intergenic 55.0%
CATAGCTGCGCTTCGTCTCT+TGG + tig0021157:111755-111774 Msa1388720:CDS 55.0%
GTTTCCACAAAGCTTCCCCG+CGG - tig0021157:111985-112004 None:intergenic 55.0%
! GGATTTTGGATGGGGTGAGG+CGG + tig0021157:112169-112188 Msa1388720:CDS 55.0%
GAGCTGCAAAAAGTGGGGCT+GGG + tig0021157:112201-112220 Msa1388720:CDS 55.0%
GGGATGATATAGGCTGCTCC+AGG - tig0021157:112233-112252 None:intergenic 55.0%
TCACGAGGAGTGGAAGCAGC+TGG - tig0021157:109399-109418 None:intergenic 60.0%
! GGAAGGATTAGGGAGGGTGC+TGG + tig0021157:109579-109598 Msa1388720:CDS 60.0%
!! GGGTGCTGGGCGTAAGTTGA+TGG + tig0021157:109593-109612 Msa1388720:CDS 60.0%
CCGCCCCATTCGACTCATAC+AGG + tig0021157:109758-109777 Msa1388720:CDS 60.0%
GCGGAGCCACACACAACATG+AGG + tig0021157:110318-110337 Msa1388720:intron 60.0%
TTGACCGGTGTGGTGAAGGG+AGG - tig0021157:111036-111055 None:intergenic 60.0%
TCCCTTCACCACACCGGTCA+AGG + tig0021157:111035-111054 Msa1388720:CDS 60.0%
CCCTTGACCGGTGTGGTGAA+GGG - tig0021157:111039-111058 None:intergenic 60.0%
CCCTTCACCACACCGGTCAA+GGG + tig0021157:111036-111055 Msa1388720:CDS 60.0%
TCCTCGAGCCATTTCTGCCC+AGG - tig0021157:111562-111581 None:intergenic 60.0%
GCATCGTCAATGCACGCTCG+AGG + tig0021157:111889-111908 Msa1388720:CDS 60.0%
GGAGCTGCAAAAAGTGGGGC+TGG + tig0021157:112200-112219 Msa1388720:CDS 60.0%
! CTGGTGGCACCAGCTCTGGT+TGG - tig0021157:109380-109399 None:intergenic 65.0%
CGAGGAGTGGAAGCAGCTGG+TGG - tig0021157:109396-109415 None:intergenic 65.0%
GCCCTTGACCGGTGTGGTGA+AGG - tig0021157:111040-111059 None:intergenic 65.0%
GATGAGGCCCTTGACCGGTG+TGG - tig0021157:111046-111065 None:intergenic 65.0%
GTGACACGCCTCAAGTGCGG+TGG + tig0021157:111468-111487 Msa1388720:CDS 65.0%
GCCTGGGCAGAAATGGCTCG+AGG + tig0021157:111558-111577 Msa1388720:CDS 65.0%
ACCACCGCGGGGAAGCTTTG+TGG + tig0021157:111978-111997 Msa1388720:CDS 65.0%
TCCACAAAGCTTCCCCGCGG+TGG - tig0021157:111982-112001 None:intergenic 65.0%
GGATGGGGTGAGGCGGTGTA+TGG + tig0021157:112176-112195 Msa1388720:CDS 65.0%
TGGGGTGAGGCGGTGTATGG+TGG + tig0021157:112179-112198 Msa1388720:CDS 65.0%
! AGTGGGGCTGGGCCTTTTCC+TGG + tig0021157:112212-112231 Msa1388720:CDS 65.0%
GCGGAGGAGCCAACCAGAGC+TGG + tig0021157:109368-109387 Msa1388720:CDS 70.0%
GCAGCTGGTGGCACCAGCTC+TGG - tig0021157:109384-109403 None:intergenic 70.0%
CCGCGGTGGTGACAGCTGCC+GGG - tig0021157:111968-111987 None:intergenic 75.0%
CCCGGCAGCTGTCACCACCG+CGG + tig0021157:111965-111984 Msa1388720:CDS 75.0%
CCCGCGGTGGTGACAGCTGC+CGG - tig0021157:111969-111988 None:intergenic 75.0%
CCGGCAGCTGTCACCACCGC+GGG + tig0021157:111966-111985 Msa1388720:CDS 75.0%
CGGCAGCTGTCACCACCGCG+GGG + tig0021157:111967-111986 Msa1388720:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0021157 gene 108994 112648 108994 ID=Msa1388720;Name=Msa1388720
tig0021157 mRNA 108994 112648 108994 ID=Msa1388720-mRNA-1;Parent=Msa1388720;Name=Msa1388720-mRNA-1;_AED=0.04;_eAED=0.04;_QI=345|0|0.66|0.66|1|1|3|284|464
tig0021157 exon 108994 109779 108994 ID=Msa1388720-mRNA-1:exon:11235;Parent=Msa1388720-mRNA-1
tig0021157 exon 111029 111085 111029 ID=Msa1388720-mRNA-1:exon:11236;Parent=Msa1388720-mRNA-1
tig0021157 exon 111468 112648 111468 ID=Msa1388720-mRNA-1:exon:11237;Parent=Msa1388720-mRNA-1
tig0021157 five_prime_UTR 108994 109338 108994 ID=Msa1388720-mRNA-1:five_prime_utr;Parent=Msa1388720-mRNA-1
tig0021157 CDS 109339 109779 109339 ID=Msa1388720-mRNA-1:cds;Parent=Msa1388720-mRNA-1
tig0021157 CDS 111029 111085 111029 ID=Msa1388720-mRNA-1:cds;Parent=Msa1388720-mRNA-1
tig0021157 CDS 111468 112364 111468 ID=Msa1388720-mRNA-1:cds;Parent=Msa1388720-mRNA-1
tig0021157 three_prime_UTR 112365 112648 112365 ID=Msa1388720-mRNA-1:three_prime_utr;Parent=Msa1388720-mRNA-1
Gene Sequence

>Msa1388720

ATGACTTCAGCACCATTATTGTTCACAGTGCGGAGGAGCCAACCAGAGCTGGTGCCACCAGCTGCTTCCACTCCTCGTGAAGTTAAACTATTATCTGACATAGATGATCAAGAAGGCTTGCGTTTCAATATTCCTATGATGTTTATCTATCGTCATGAACCGTCAATGAAAGAAAAAAACCCTATTAAAGTTCTTAGACATGCACTCTCACAAGCACTTGTGTATTACTATCCATTTGCAGGAAGGATTAGGGAGGGTGCTGGGCGTAAGTTGATGGTGGATTGTACTGGTGAGGGTGTCATGTTCATTGAAGCTGAAGCTGATGTAACACTTGATCAATTTGGGGACGCTTTGCATCCCCCATTCCCATGCTTCAAAGAACTTCTATACGATGTTCCAGGATCAGAACTAATTATTGACCGCCCCATTCGACTCATACAGTCTACCTCCCTTCACCACACCGGTCAAGGGCCTCATCGTAAGAATGGTTCAACGAGGGTGACACGCCTCAAGTGCGGTGGTTTCATGCTAGTCGTGTATTTGAACCATACAATGAGCGATGGAGCTGGTTTGAAACTATTCATGAATGCCTGGGCAGAAATGGCTCGAGGAGCACATAAACCTTCCATTCAACCTGTATGGAATAGGGAGATTCTAATGGCAAGAGACCCACCACACATTACATGCAACCATCATGAATACGAACATATTTTTTCGTCAAATACCATCAAGGAAGAAGATACTACAACCCTGGTTCATCAATCTTTCTTCTTTAGAACTTCTGACATAGCTGCGCTTCGTCTCTTGGTTCCATCTCACCTTCGTCATTGTACGACATTCGATCTTATCACTTCATGTTTTTGGTGCTGCCGCACAAAAGCATTGCAGTTAGAGGCAGATGAAGAGATACGAATGATGTGCATCGTCAATGCACGCTCGAGGTTTAGCGTCAACCATTCGCCACTTGTTGGATATTACGGTAATTGTTTTGCTTACCCGGCAGCTGTCACCACCGCGGGGAAGCTTTGTGGAAACTCATTAGGTTATGCTGTGGAGTTGGTGAGAAAATTAAAAGCTGAAGTTACAGAGGAATACATGCATTCTGTAGCAGATCTTATGGTTATTAAGGAACGGTGTTTATTTACAACTACAAGGTCTTGTGTTGTTTCAGATGTGACTCGTATAAAGTTCAAAGAAGTGGATTTTGGATGGGGTGAGGCGGTGTATGGTGGAGCTGCAAAAAGTGGGGCTGGGCCTTTTCCTGGAGCAGCCTATATCATCCCACATAAGAATGTAAAAGGAGAAGAAGGTTTCATATTACTAGTTTGTTTGTCCTTTGAAGATATGAAGAGGTTTGCTAAAGAGTTAGATGAGATGCTTGGTAAACAAAATTAA
Protein sequence

>Msa1388720

MTSAPLLFTVRRSQPELVPPAASTPREVKLLSDIDDQEGLRFNIPMMFIYRHEPSMKEKNPIKVLRHALSQALVYYYPFAGRIREGAGRKLMVDCTGEGVMFIEAEADVTLDQFGDALHPPFPCFKELLYDVPGSELIIDRPIRLIQSTSLHHTGQGPHRKNGSTRVTRLKCGGFMLVVYLNHTMSDGAGLKLFMNAWAEMARGAHKPSIQPVWNREILMARDPPHITCNHHEYEHIFSSNTIKEEDTTTLVHQSFFFRTSDIAALRLLVPSHLRHCTTFDLITSCFWCCRTKALQLEADEEIRMMCIVNARSRFSVNHSPLVGYYGNCFAYPAAVTTAGKLCGNSLGYAVELVRKLKAEVTEEYMHSVADLMVIKERCLFTTTRSCVVSDVTRIKFKEVDFGWGEAVYGGAAKSGAGPFPGAAYIIPHKNVKGEEGFILLVCLSFEDMKRFAKELDEMLGKQN*