AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0178950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0178950 MtrunA17_Chr1g0208181 96.933 163 5 0 61 223 1 163 6.78e-113 320
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0178950 AT2G01640.2 54.483 145 56 6 61 202 1 138 7.43e-37 127
Msa0178950 AT2G01640.1 54.483 145 56 6 61 202 1 138 7.43e-37 127

Find 55 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGCTGCTGGTGTGAATT+AGG 0.127146 1_4:+66386490 None:intergenic
TCTGCTGCTGGTGTGAATTA+GGG 0.265607 1_4:+66386491 None:intergenic
TGTTTCTCTGCTACTGGTTC+TGG 0.277305 1_4:+66384367 None:intergenic
CTCTTGTATGGATGCTATTA+TGG 0.318660 1_4:-66384250 Msa0178950:three_prime_UTR
TTCGCTCTTGGATACACTTC+CGG 0.357680 1_4:-66384654 Msa0178950:CDS
CTCCAGATCTGGACAGGGGT+GGG 0.361292 1_4:-66387267 Msa0178950:CDS
GGTTGTTGTTTCTCTGCTAC+TGG 0.367383 1_4:+66384361 None:intergenic
CAGGGGTGGGGTGACGTTGG+TGG 0.390072 1_4:-66387254 Msa0178950:CDS
CTGCCATTCATCAACACTTA+AGG 0.392107 1_4:-66384399 Msa0178950:CDS
ACCCCACCCCTGTCCAGATC+TGG 0.408689 1_4:+66387265 None:intergenic
TGTGTGCAGGTCAGATTCAT+TGG 0.408805 1_4:-66386170 Msa0178950:intron
GATATATAATGCCTCTTGTA+TGG 0.418588 1_4:-66384262 Msa0178950:exon
TCCAGATCTGGACAGGGGTG+GGG 0.423717 1_4:-66387266 Msa0178950:CDS
GGGTGGGGTGACGTTGGTGG+TGG 0.435880 1_4:-66387251 Msa0178950:CDS
ATATAACCTCTCTTCGCTCT+TGG 0.450344 1_4:-66384666 Msa0178950:CDS
TCTCCAGATCTGGACAGGGG+TGG 0.458282 1_4:-66387268 Msa0178950:CDS
TGGGGCTGGACTGAAGTCTA+TGG 0.458997 1_4:-66384284 Msa0178950:CDS
TATGCTTGATAAATTTAAGA+TGG 0.468387 1_4:+66384130 None:intergenic
GTAATTATTCATCAGATTGA+AGG 0.473506 1_4:-66384479 Msa0178950:intron
TTTCATACGGTTGCCATGCT+CGG 0.501474 1_4:+66384033 None:intergenic
ACATATGTGTTAGTTTCATA+CGG 0.502585 1_4:+66384020 None:intergenic
GTCCTCGTTTCTCTGTTCAC+AGG 0.509614 1_4:-66386458 Msa0178950:intron
TTCTGTGGTGCATTCAACTC+CGG 0.528288 1_4:+66384635 None:intergenic
TCCTCATTGCATGGTTTCTG+TGG 0.529876 1_4:+66384620 None:intergenic
ATGCAATGAGGATAACTTCA+AGG 0.532567 1_4:-66384609 Msa0178950:CDS
TTCATTCCAAGTGAATCCCT+TGG 0.548105 1_4:-66384422 Msa0178950:CDS
GTGTTGATGAATGGCAGCCA+AGG 0.549692 1_4:+66384405 None:intergenic
CGGTTGCCATGCTCGGCAGA+TGG 0.551580 1_4:+66384040 None:intergenic
GGTGGGAGGATGGAGGCCGC+CGG 0.553373 1_4:-66387303 Msa0178950:CDS
TTGAAGTTATCCTCATTGCA+TGG 0.556750 1_4:+66384611 None:intergenic
GGACAGGGGTGGGGTGACGT+TGG 0.558325 1_4:-66387257 Msa0178950:CDS
AGATTCATTGGATTCAAAGT+CGG 0.561288 1_4:-66386158 Msa0178950:CDS
CTGGAGAAAATCGTCATAGC+CGG 0.579757 1_4:+66387284 None:intergenic
AGTTGACCATCTGCCGAGCA+TGG 0.579873 1_4:-66384046 Msa0178950:three_prime_UTR
GTCTACAGTGATAATGTCCA+TGG 0.593003 1_4:-66384107 Msa0178950:three_prime_UTR
AATAGACTTTGTGACGGTCA+AGG 0.595183 1_4:+66385818 None:intergenic
TGGCAGCCAAGGGATTCACT+TGG 0.596398 1_4:+66384416 None:intergenic
GCTCCTTAAGTGTTGATGAA+TGG 0.602260 1_4:+66384396 None:intergenic
AGAATCAGAGAAAACATCAA+AGG 0.605134 1_4:-66384709 Msa0178950:CDS
CGTCACAAAGTCTATTTCGA+AGG 0.606011 1_4:-66385811 Msa0178950:intron
TGTTGATGAATGGCAGCCAA+GGG 0.606578 1_4:+66384406 None:intergenic
AACTTAGAGTATGAAGAAAG+CGG 0.608436 1_4:+66386311 None:intergenic
CAGATGGTCAACTTACCCAA+TGG 0.609213 1_4:+66384056 None:intergenic
AACAGAGAAACGAGGACGAG+AGG 0.614416 1_4:+66386464 None:intergenic
TTACGATGGTGGGAGGATGG+AGG 0.615118 1_4:-66387310 None:intergenic
CAATGGGGAAGACAAATACA+AGG 0.620450 1_4:-66386277 Msa0178950:intron
CATAATAGCATCCATACAAG+AGG 0.622729 1_4:+66384251 None:intergenic
CTTCGAAATAGACTTTGTGA+CGG 0.636071 1_4:+66385812 None:intergenic
AAAATAGTGACAAACTACCA+TGG 0.639465 1_4:+66384090 None:intergenic
TTATTCATCAGATTGAAGGA+AGG 0.642626 1_4:-66384475 Msa0178950:intron
ATCAGAGAAAACATCAAAGG+CGG 0.644411 1_4:-66384706 Msa0178950:CDS
GTGTATCCAAGAGCGAAGAG+AGG 0.658487 1_4:+66384660 None:intergenic
GAGAAAATCGTCATAGCCGG+CGG 0.665597 1_4:+66387287 None:intergenic
ACCACAGAAACCATGCAATG+AGG 0.679684 1_4:-66384621 Msa0178950:CDS
TACCTGTGAACAGAGAAACG+AGG 0.809595 1_4:+66386456 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTTCATAAAAAATGAA+GGG + chr1_4:66384536-66384555 None:intergenic 10.0%
!! TATTATTTCATAAAAAATGA+AGG + chr1_4:66384537-66384556 None:intergenic 10.0%
!! AATTATTCCATTTAAAAATT+TGG - chr1_4:66384624-66384643 Msa0178950:CDS 10.0%
!! TTCAAAAAAAAAAAAACATA+TGG + chr1_4:66384737-66384756 None:intergenic 10.0%
!! CTTAAAATATTTATCAAAAT+TGG + chr1_4:66386213-66386232 None:intergenic 10.0%
!!! ATTTTGATAAATATTTTAAG+AGG - chr1_4:66386213-66386232 Msa0178950:intron 10.0%
!!! ACTATTTTTTTTTTTTTTTG+TGG - chr1_4:66387137-66387156 Msa0178950:intron 10.0%
!! ATTAATAAAAAATGCCATAT+TGG - chr1_4:66384345-66384364 Msa0178950:CDS 15.0%
!! AAATGATAAATAAAACCATA+TGG + chr1_4:66384767-66384786 None:intergenic 15.0%
!!! AAGTCTCAATTTTTATTTAT+CGG + chr1_4:66384808-66384827 None:intergenic 15.0%
!!! TAAAAATTGAGACTTTTAAA+CGG - chr1_4:66384813-66384832 Msa0178950:intron 15.0%
!!! ATATGAAGAACTTTAAAAAA+GGG + chr1_4:66387111-66387130 None:intergenic 15.0%
!!! TATATGAAGAACTTTAAAAA+AGG + chr1_4:66387112-66387131 None:intergenic 15.0%
!! ATACTAATGAATCAAAAAGA+AGG + chr1_4:66384496-66384515 None:intergenic 20.0%
!!! AGTCATTCCAAATTTTTAAA+TGG + chr1_4:66384634-66384653 None:intergenic 20.0%
!!! TTTTTGAAATACAAACCATA+TGG - chr1_4:66384749-66384768 Msa0178950:intron 20.0%
!!! AAAAATTGAGACTTTTAAAC+GGG - chr1_4:66384814-66384833 Msa0178950:intron 20.0%
!!! TAGAAAGTAACTTTGATTTT+CGG + chr1_4:66385099-66385118 None:intergenic 20.0%
!!! TTCTATCATAAACCAAATTT+TGG + chr1_4:66385846-66385865 None:intergenic 20.0%
!! GTTGCAATTTAATATCAAAT+GGG + chr1_4:66386036-66386055 None:intergenic 20.0%
!! AGTTGCAATTTAATATCAAA+TGG + chr1_4:66386037-66386056 None:intergenic 20.0%
!! CAGAAAATCAATAATATTCA+TGG + chr1_4:66386586-66386605 None:intergenic 20.0%
!! TTTACATATCAAATGTATAG+AGG + chr1_4:66386815-66386834 None:intergenic 20.0%
!!! TATGAAGAACTTTAAAAAAG+GGG + chr1_4:66387110-66387129 None:intergenic 20.0%
!! TATGCTTGATAAATTTAAGA+TGG + chr1_4:66387206-66387225 None:intergenic 20.0%
!!! GTTTGTCACTATTTTTATAT+CGG - chr1_4:66387251-66387270 Msa0178950:CDS 20.0%
! GAAAAAATTGTTGAATCTGT+TGG - chr1_4:66384273-66384292 Msa0178950:exon 25.0%
!!! ATGCCTCTTAATTTTTTAAG+TGG - chr1_4:66384302-66384321 Msa0178950:CDS 25.0%
! TGTCCACTTAAAAAATTAAG+AGG + chr1_4:66384308-66384327 None:intergenic 25.0%
!! AGACTTTTGTCAAAAAAAGA+AGG + chr1_4:66384472-66384491 None:intergenic 25.0%
!! TTCCCTTCTAATTTTCTTTT+GGG - chr1_4:66384919-66384938 Msa0178950:intron 25.0%
! AACCCAAAAGAAAATTAGAA+GGG + chr1_4:66384924-66384943 None:intergenic 25.0%
! AAACCCAAAAGAAAATTAGA+AGG + chr1_4:66384925-66384944 None:intergenic 25.0%
!!! TAAGTTTTATCTTATCGCAA+TGG - chr1_4:66385039-66385058 Msa0178950:intron 25.0%
!!! AAGTTTTATCTTATCGCAAT+GGG - chr1_4:66385040-66385059 Msa0178950:intron 25.0%
! AATTCTATACCAGTAAGTTT+GGG - chr1_4:66385222-66385241 Msa0178950:intron 25.0%
! TGTAATTCGAAAAAACTTGA+TGG + chr1_4:66385248-66385267 None:intergenic 25.0%
!! AATTCAACTGTTTTGTCATT+AGG + chr1_4:66385298-66385317 None:intergenic 25.0%
! CTAATTAACTTCATTATGCT+GGG - chr1_4:66385582-66385601 Msa0178950:intron 25.0%
! TAACATCAATTCACAATCAA+TGG + chr1_4:66385617-66385636 None:intergenic 25.0%
! TGGTTTATGATAGAACATTA+TGG - chr1_4:66385851-66385870 Msa0178950:intron 25.0%
! CAAGATTTAGCATTTAAACA+CGG + chr1_4:66385963-66385982 None:intergenic 25.0%
!! GATGCATTAGTATAATTTTG+TGG - chr1_4:66386005-66386024 Msa0178950:intron 25.0%
! ATATTAAATTGCAACTCACT+TGG - chr1_4:66386041-66386060 Msa0178950:intron 25.0%
! AAGATGTTTGTAAGTGTTTA+TGG + chr1_4:66386331-66386350 None:intergenic 25.0%
! TAAACACTTACAAACATCTT+AGG - chr1_4:66386331-66386350 Msa0178950:intron 25.0%
! ATAGTTCTTCACTTATGAAA+AGG + chr1_4:66386365-66386384 None:intergenic 25.0%
!!! TTGGATTAGTTCTTAAATGA+TGG + chr1_4:66386415-66386434 None:intergenic 25.0%
!! ATAATTAGTATGTTCTGGTT+TGG + chr1_4:66386434-66386453 None:intergenic 25.0%
! TTGAGATAATTAGTATGTTC+TGG + chr1_4:66386439-66386458 None:intergenic 25.0%
! ATACATATCAAAAAGAACCT+TGG + chr1_4:66386540-66386559 None:intergenic 25.0%
! GTAATTATTCATCAGATTGA+AGG - chr1_4:66386854-66386873 Msa0178950:intron 25.0%
!!! TTTACATTCGCTTTTTTCTT+GGG + chr1_4:66386996-66387015 None:intergenic 25.0%
!!! ATTTACATTCGCTTTTTTCT+TGG + chr1_4:66386997-66387016 None:intergenic 25.0%
! AAGAAAAAAGCGAATGTAAA+TGG - chr1_4:66386996-66387015 Msa0178950:intron 25.0%
! AGAAAAAAGCGAATGTAAAT+GGG - chr1_4:66386997-66387016 Msa0178950:intron 25.0%
AAATGATATAGGGTTTCTGA+CGG - chr1_4:66384148-66384167 Msa0178950:three_prime_UTR 30.0%
TAAAAAATGCCATATTGGAG+TGG - chr1_4:66384350-66384369 Msa0178950:CDS 30.0%
GATATATAGACATGTTTAGG+TGG + chr1_4:66384391-66384410 None:intergenic 30.0%
GGTGATATATAGACATGTTT+AGG + chr1_4:66384394-66384413 None:intergenic 30.0%
GTCTTCTGTAAAAGAAAATG+TGG + chr1_4:66384711-66384730 None:intergenic 30.0%
! CTTCCCTTCTAATTTTCTTT+TGG - chr1_4:66384918-66384937 Msa0178950:intron 30.0%
!!! GCTTGTGTTTGTTTTGTAAT+AGG - chr1_4:66384996-66385015 Msa0178950:intron 30.0%
AACTTAGAGTATGAAGAAAG+CGG + chr1_4:66385025-66385044 None:intergenic 30.0%
!! AGTTTTATCTTATCGCAATG+GGG - chr1_4:66385041-66385060 Msa0178950:intron 30.0%
GGAATCAGATTTGCATAAAT+GGG + chr1_4:66385133-66385152 None:intergenic 30.0%
AGATTCATTGGATTCAAAGT+CGG - chr1_4:66385175-66385194 Msa0178950:intron 30.0%
CAATTCTATACCAGTAAGTT+TGG - chr1_4:66385221-66385240 Msa0178950:intron 30.0%
ATGTGTATGAAGTTTATCGT+TGG - chr1_4:66385404-66385423 Msa0178950:intron 30.0%
GCTAATTAACTTCATTATGC+TGG - chr1_4:66385581-66385600 Msa0178950:intron 30.0%
! CATAATGAACAAGAAAGTAC+TGG + chr1_4:66385667-66385686 None:intergenic 30.0%
! TTTTCCAACAACTTCAGATT+AGG - chr1_4:66385733-66385752 Msa0178950:intron 30.0%
! TTGGTAGAATTGATAGTAAC+TGG - chr1_4:66386060-66386079 Msa0178950:intron 30.0%
! TGGTAGAATTGATAGTAACT+GGG - chr1_4:66386061-66386080 Msa0178950:intron 30.0%
TTACAAACATCTTAGGAGTA+AGG - chr1_4:66386338-66386357 Msa0178950:intron 30.0%
! GATTAGTTCTTAAATGATGG+AGG + chr1_4:66386412-66386431 None:intergenic 30.0%
TATATAGAAATTGTGCGATC+AGG + chr1_4:66386494-66386513 None:intergenic 30.0%
AGAATCAGAGAAAACATCAA+AGG - chr1_4:66386624-66386643 Msa0178950:intron 30.0%
AAAATAGTATTTGTGACCCT+CGG - chr1_4:66386832-66386851 Msa0178950:intron 30.0%
ATCTGATGAATAATTACCGA+GGG + chr1_4:66386851-66386870 None:intergenic 30.0%
AATCTGATGAATAATTACCG+AGG + chr1_4:66386852-66386871 None:intergenic 30.0%
TTATTCATCAGATTGAAGGA+AGG - chr1_4:66386858-66386877 Msa0178950:intron 30.0%
ATGTAAATGGGTCAAAAAAG+AGG - chr1_4:66387009-66387028 Msa0178950:intron 30.0%
AGGAAGAAAAAATCAAAAGC+TGG - chr1_4:66387029-66387048 Msa0178950:intron 30.0%
GGAAGAAAAAATCAAAAGCT+GGG - chr1_4:66387030-66387049 Msa0178950:intron 30.0%
GAAGAAAAAATCAAAAGCTG+GGG - chr1_4:66387031-66387050 Msa0178950:intron 30.0%
GATATATAATGCCTCTTGTA+TGG - chr1_4:66387071-66387090 Msa0178950:intron 30.0%
AAAATAGTGACAAACTACCA+TGG + chr1_4:66387246-66387265 None:intergenic 30.0%
! ATTTTTATATCGGCATCCAT+TGG - chr1_4:66387261-66387280 Msa0178950:CDS 30.0%
! TTTTTATATCGGCATCCATT+GGG - chr1_4:66387262-66387281 Msa0178950:CDS 30.0%
GGGAGGAAACAAATGATATA+GGG - chr1_4:66384138-66384157 Msa0178950:three_prime_UTR 35.0%
CGGAATCAGATTTGCATAAA+TGG + chr1_4:66385134-66385153 None:intergenic 35.0%
!!! TGAAGTACTGGTTTTGTGTA+AGG - chr1_4:66385354-66385373 Msa0178950:intron 35.0%
TGTATGAAGTTTATCGTTGG+TGG - chr1_4:66385407-66385426 Msa0178950:intron 35.0%
CTTCGAAATAGACTTTGTGA+CGG + chr1_4:66385524-66385543 None:intergenic 35.0%
GGTAAAAATCTCATTGTCAG+CGG - chr1_4:66385543-66385562 Msa0178950:intron 35.0%
TTCTTGTTCATTATGCTTGC+TGG - chr1_4:66385672-66385691 Msa0178950:intron 35.0%
TCTTGTTCATTATGCTTGCT+GGG - chr1_4:66385673-66385692 Msa0178950:intron 35.0%
! TTGTCCTAATCTGAAGTTGT+TGG + chr1_4:66385740-66385759 None:intergenic 35.0%
AACAACTTCAGATTAGGACA+AGG - chr1_4:66385739-66385758 Msa0178950:intron 35.0%
TAAGATATGACTATCAGAGC+AGG + chr1_4:66385792-66385811 None:intergenic 35.0%
GTTCATGTGATTTAACGAGT+TGG - chr1_4:66385819-66385838 Msa0178950:CDS 35.0%
! TAACGAGTTGGACCAAAATT+TGG - chr1_4:66385831-66385850 Msa0178950:CDS 35.0%
!! ACAAAGTACTGTTAAGCTTC+AGG + chr1_4:66386179-66386198 None:intergenic 35.0%
! TAGTTCTTAAATGATGGAGG+AGG + chr1_4:66386409-66386428 None:intergenic 35.0%
ATCAGAGAAAACATCAAAGG+CGG - chr1_4:66386627-66386646 Msa0178950:intron 35.0%
TTGAAGTTATCCTCATTGCA+TGG + chr1_4:66386725-66386744 None:intergenic 35.0%
ATGCAATGAGGATAACTTCA+AGG - chr1_4:66386724-66386743 Msa0178950:intron 35.0%
CATAATAGCATCCATACAAG+AGG + chr1_4:66387085-66387104 None:intergenic 35.0%
CTCTTGTATGGATGCTATTA+TGG - chr1_4:66387083-66387102 Msa0178950:intron 35.0%
! ATGACGATTTTCTCCAGATC+TGG - chr1_4:66384055-66384074 Msa0178950:three_prime_UTR 40.0%
! ATTTTCTCCAGATCTGGACA+GGG - chr1_4:66384061-66384080 Msa0178950:three_prime_UTR 40.0%
GGGGAGGAAACAAATGATAT+AGG - chr1_4:66384137-66384156 Msa0178950:three_prime_UTR 40.0%
GGGATGAAAGAGAGAAAAGA+GGG - chr1_4:66384249-66384268 Msa0178950:three_prime_UTR 40.0%
ACCATTGTACCACTCCAATA+TGG + chr1_4:66384362-66384381 None:intergenic 40.0%
ATATTGGAGTGGTACAATGG+TGG - chr1_4:66384361-66384380 Msa0178950:CDS 40.0%
ATGGAGGTCTTCAATTTGAG+TGG + chr1_4:66384415-66384434 None:intergenic 40.0%
AGAAGGAAGACTTGTGATGT+TGG + chr1_4:66384455-66384474 None:intergenic 40.0%
CAATGGGGAAGACAAATACA+AGG - chr1_4:66385056-66385075 Msa0178950:intron 40.0%
AAACGCTATTGCTGAAGTAC+TGG - chr1_4:66385342-66385361 Msa0178950:intron 40.0%
!! GAAGTTTATCGTTGGTGGTT+TGG - chr1_4:66385412-66385431 Msa0178950:intron 40.0%
CAAAAACAGCAGAAGTTGTG+AGG + chr1_4:66385471-66385490 None:intergenic 40.0%
!!! AACTTCTGCTGTTTTTGCAG+AGG - chr1_4:66385474-66385493 Msa0178950:intron 40.0%
AATAGACTTTGTGACGGTCA+AGG + chr1_4:66385518-66385537 None:intergenic 40.0%
CGTCACAAAGTCTATTTCGA+AGG - chr1_4:66385522-66385541 Msa0178950:intron 40.0%
AAAAATCTCATTGTCAGCGG+AGG - chr1_4:66385546-66385565 Msa0178950:intron 40.0%
!! GAAAGTACTGGAACATGTCT+GGG + chr1_4:66385655-66385674 None:intergenic 40.0%
!! AGAAAGTACTGGAACATGTC+TGG + chr1_4:66385656-66385675 None:intergenic 40.0%
AGTACTGTTAAGCTTCAGGT+CGG + chr1_4:66386175-66386194 None:intergenic 40.0%
CCTTGGTTCAGTTAGTAACT+TGG + chr1_4:66386523-66386542 None:intergenic 40.0%
CCAAGTTACTAACTGAACCA+AGG - chr1_4:66386520-66386539 Msa0178950:intron 40.0%
ATATAACCTCTCTTCGCTCT+TGG - chr1_4:66386667-66386686 Msa0178950:intron 40.0%
TTCATTCCAAGTGAATCCCT+TGG - chr1_4:66386911-66386930 Msa0178950:intron 40.0%
CTGCCATTCATCAACACTTA+AGG - chr1_4:66386934-66386953 Msa0178950:intron 40.0%
!! GCTCCTTAAGTGTTGATGAA+TGG + chr1_4:66386940-66386959 None:intergenic 40.0%
AAAAAATCAAAAGCTGGGGC+TGG - chr1_4:66387035-66387054 Msa0178950:intron 40.0%
! GTCTACAGTGATAATGTCCA+TGG - chr1_4:66387226-66387245 Msa0178950:intron 40.0%
CTGGAGAAAATCGTCATAGC+CGG + chr1_4:66384052-66384071 None:intergenic 45.0%
! GATTTTCTCCAGATCTGGAC+AGG - chr1_4:66384060-66384079 Msa0178950:three_prime_UTR 45.0%
! TTTTCTCCAGATCTGGACAG+GGG - chr1_4:66384062-66384081 Msa0178950:three_prime_UTR 45.0%
GAATGTGAGGAAGAGAGAAC+AGG - chr1_4:66384107-66384126 Msa0178950:three_prime_UTR 45.0%
AGGAAGAGAGAACAGGAAGT+TGG - chr1_4:66384114-66384133 Msa0178950:three_prime_UTR 45.0%
GGAAGAGAGAACAGGAAGTT+GGG - chr1_4:66384115-66384134 Msa0178950:three_prime_UTR 45.0%
GAAGAGAGAACAGGAAGTTG+GGG - chr1_4:66384116-66384135 Msa0178950:three_prime_UTR 45.0%
AAGAGAGAACAGGAAGTTGG+GGG - chr1_4:66384117-66384136 Msa0178950:three_prime_UTR 45.0%
TGATATAGGGTTTCTGACGG+AGG - chr1_4:66384151-66384170 Msa0178950:three_prime_UTR 45.0%
GATATAGGGTTTCTGACGGA+GGG - chr1_4:66384152-66384171 Msa0178950:three_prime_UTR 45.0%
ATATAGGGTTTCTGACGGAG+GGG - chr1_4:66384153-66384172 Msa0178950:three_prime_UTR 45.0%
TTCTCTCTTTCATCCCCTCA+CGG + chr1_4:66384245-66384264 None:intergenic 45.0%
GGGGATGAAAGAGAGAAAAG+AGG - chr1_4:66384248-66384267 Msa0178950:three_prime_UTR 45.0%
GCCATATTGGAGTGGTACAA+TGG - chr1_4:66384358-66384377 Msa0178950:CDS 45.0%
CAAATTGAAGACCTCCATCG+TGG - chr1_4:66384417-66384436 Msa0178950:CDS 45.0%
AGACTTGTGATGTTGGTCCT+TGG + chr1_4:66384448-66384467 None:intergenic 45.0%
TCTGCTGCTGGTGTGAATTA+GGG + chr1_4:66384845-66384864 None:intergenic 45.0%
TTCTGCTGCTGGTGTGAATT+AGG + chr1_4:66384846-66384865 None:intergenic 45.0%
TACCTGTGAACAGAGAAACG+AGG + chr1_4:66384880-66384899 None:intergenic 45.0%
ACCTGCACACATGAGAAACT+CGG + chr1_4:66385154-66385173 None:intergenic 45.0%
TGTGTGCAGGTCAGATTCAT+TGG - chr1_4:66385163-66385182 Msa0178950:intron 45.0%
! ACTTGATGGCCCAAACTTAC+TGG + chr1_4:66385234-66385253 None:intergenic 45.0%
ATATGACTATCAGAGCAGGC+AGG + chr1_4:66385788-66385807 None:intergenic 45.0%
GTACTGTTAAGCTTCAGGTC+GGG + chr1_4:66386174-66386193 None:intergenic 45.0%
TTCGCTCTTGGATACACTTC+CGG - chr1_4:66386679-66386698 Msa0178950:intron 45.0%
TTCTGTGGTGCATTCAACTC+CGG + chr1_4:66386701-66386720 None:intergenic 45.0%
ACCACAGAAACCATGCAATG+AGG - chr1_4:66386712-66386731 Msa0178950:intron 45.0%
TCCTCATTGCATGGTTTCTG+TGG + chr1_4:66386716-66386735 None:intergenic 45.0%
! TGTTGATGAATGGCAGCCAA+GGG + chr1_4:66386930-66386949 None:intergenic 45.0%
!! TGTTTCTCTGCTACTGGTTC+TGG + chr1_4:66386969-66386988 None:intergenic 45.0%
!! GGTTGTTGTTTCTCTGCTAC+TGG + chr1_4:66386975-66386994 None:intergenic 45.0%
CAGATGGTCAACTTACCCAA+TGG + chr1_4:66387280-66387299 None:intergenic 45.0%
TTTCATACGGTTGCCATGCT+CGG + chr1_4:66387303-66387322 None:intergenic 45.0%
!!! TTTTTTATTAATTATTATGA+GGG + chr1_4:66384337-66384356 None:intergenic 5.0%
!!! ATTTTTTATTAATTATTATG+AGG + chr1_4:66384338-66384357 None:intergenic 5.0%
GAGAAAATCGTCATAGCCGG+CGG + chr1_4:66384049-66384068 None:intergenic 50.0%
AGAGAGAACAGGAAGTTGGG+GGG - chr1_4:66384118-66384137 Msa0178950:three_prime_UTR 50.0%
AACAGAGAAACGAGGACGAG+AGG + chr1_4:66384872-66384891 None:intergenic 50.0%
GTCCTCGTTTCTCTGTTCAC+AGG - chr1_4:66384875-66384894 Msa0178950:intron 50.0%
TCCGAGTTTCTCATGTGTGC+AGG - chr1_4:66385150-66385169 Msa0178950:intron 50.0%
TCAGCGGAGGCTATGGTTAA+TGG - chr1_4:66385559-66385578 Msa0178950:intron 50.0%
CTAAGTGGGGTCGACTACAT+GGG + chr1_4:66385887-66385906 None:intergenic 50.0%
ATGTAGTCGACCCCACTTAG+CGG - chr1_4:66385887-66385906 Msa0178950:intron 50.0%
! AAGCCTTTTCCCGCTAAGTG+GGG + chr1_4:66385900-66385919 None:intergenic 50.0%
! CAAGCCTTTTCCCGCTAAGT+GGG + chr1_4:66385901-66385920 None:intergenic 50.0%
! TCAAGCCTTTTCCCGCTAAG+TGG + chr1_4:66385902-66385921 None:intergenic 50.0%
GTGTATCCAAGAGCGAAGAG+AGG + chr1_4:66386676-66386695 None:intergenic 50.0%
! GTGTTGATGAATGGCAGCCA+AGG + chr1_4:66386931-66386950 None:intergenic 50.0%
!! GTTGGTGGTGGTCGAATGTG+AGG - chr1_4:66384094-66384113 Msa0178950:three_prime_UTR 55.0%
! GAAGGTAGAGGTCTGAGGTG+AGG - chr1_4:66384181-66384200 Msa0178950:three_prime_UTR 55.0%
! AGGTCTGAGGTGAGGAGAGA+GGG - chr1_4:66384189-66384208 Msa0178950:three_prime_UTR 55.0%
GAGAGAGGGAGAAGGTCTGA+TGG - chr1_4:66384203-66384222 Msa0178950:three_prime_UTR 55.0%
! GGGAGAAGGTCTGATGGTGA+CGG - chr1_4:66384209-66384228 Msa0178950:three_prime_UTR 55.0%
!! AGAAGGTCTGATGGTGACGG+TGG - chr1_4:66384212-66384231 Msa0178950:three_prime_UTR 55.0%
! CCTTGGAGTATCCACGATGG+AGG + chr1_4:66384431-66384450 None:intergenic 55.0%
CCTCCATCGTGGATACTCCA+AGG - chr1_4:66384428-66384447 Msa0178950:CDS 55.0%
! GGTCCTTGGAGTATCCACGA+TGG + chr1_4:66384434-66384453 None:intergenic 55.0%
! ACGAGAGGCTTTTCTGCTGC+TGG + chr1_4:66384857-66384876 None:intergenic 55.0%
CTCATTGTCAGCGGAGGCTA+TGG - chr1_4:66385552-66385571 Msa0178950:intron 55.0%
GCTAAGTGGGGTCGACTACA+TGG + chr1_4:66385888-66385907 None:intergenic 55.0%
TGTAGTCGACCCCACTTAGC+GGG - chr1_4:66385888-66385907 Msa0178950:intron 55.0%
TGGCAGCCAAGGGATTCACT+TGG + chr1_4:66386920-66386939 None:intergenic 55.0%
TGGGGCTGGACTGAAGTCTA+TGG - chr1_4:66387049-66387068 Msa0178950:intron 55.0%
AGTTGACCATCTGCCGAGCA+TGG - chr1_4:66387287-66387306 Msa0178950:CDS 55.0%
TCTCCAGATCTGGACAGGGG+TGG - chr1_4:66384065-66384084 Msa0178950:three_prime_UTR 60.0%
CTCCAGATCTGGACAGGGGT+GGG - chr1_4:66384066-66384085 Msa0178950:three_prime_UTR 60.0%
TCCAGATCTGGACAGGGGTG+GGG - chr1_4:66384067-66384086 Msa0178950:three_prime_UTR 60.0%
GAGAACAGGAAGTTGGGGGG+AGG - chr1_4:66384121-66384140 Msa0178950:three_prime_UTR 60.0%
TAGGGTTTCTGACGGAGGGG+CGG - chr1_4:66384156-66384175 Msa0178950:three_prime_UTR 60.0%
! GAGGTCTGAGGTGAGGAGAG+AGG - chr1_4:66384188-66384207 Msa0178950:three_prime_UTR 60.0%
GAGGTGAGGAGAGAGGGAGA+AGG - chr1_4:66384195-66384214 Msa0178950:three_prime_UTR 60.0%
!! AGGTCTGATGGTGACGGTGG+TGG - chr1_4:66384215-66384234 Msa0178950:three_prime_UTR 60.0%
!! TCTGATGGTGACGGTGGTGG+TGG - chr1_4:66384218-66384237 Msa0178950:three_prime_UTR 60.0%
CGACCCCACTTAGCGGGAAA+AGG - chr1_4:66385894-66385913 Msa0178950:intron 60.0%
ACCCCACCCCTGTCCAGATC+TGG + chr1_4:66384071-66384090 None:intergenic 65.0%
CGGCGGAAGGTAGAGGTCTG+AGG - chr1_4:66384176-66384195 Msa0178950:three_prime_UTR 65.0%
!! ACGGTGGTGGTGGTCCGTGA+GGG - chr1_4:66384228-66384247 Msa0178950:three_prime_UTR 65.0%
CGGTTGCCATGCTCGGCAGA+TGG + chr1_4:66387296-66387315 None:intergenic 65.0%
GGACAGGGGTGGGGTGACGT+TGG - chr1_4:66384076-66384095 Msa0178950:three_prime_UTR 70.0%
CAGGGGTGGGGTGACGTTGG+TGG - chr1_4:66384079-66384098 Msa0178950:three_prime_UTR 70.0%
! GGGTGGGGTGACGTTGGTGG+TGG - chr1_4:66384082-66384101 Msa0178950:three_prime_UTR 70.0%
GGTTTCTGACGGAGGGGCGG+CGG - chr1_4:66384159-66384178 Msa0178950:three_prime_UTR 70.0%
!! GACGGTGGTGGTGGTCCGTG+AGG - chr1_4:66384227-66384246 Msa0178950:three_prime_UTR 70.0%
!! CGGTGGTGGTGGTCCGTGAG+GGG - chr1_4:66384229-66384248 Msa0178950:three_prime_UTR 70.0%
GGTGGGAGGATGGAGGCCGC+CGG - chr1_4:66384030-66384049 Msa0178950:three_prime_UTR 75.0%
TCTGACGGAGGGGCGGCGGA+AGG - chr1_4:66384163-66384182 Msa0178950:three_prime_UTR 75.0%
GGAGGGGCGGCGGAAGGTAG+AGG - chr1_4:66384169-66384188 Msa0178950:three_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 66384028 66387327 66384028 ID=Msa0178950;Name=Msa0178950
chr1_4 mRNA 66384028 66387327 66384028 ID=Msa0178950-mRNA-1;Parent=Msa0178950;Name=Msa0178950-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0.83|0.71|0.85|1|1|7|248|223
chr1_4 exon 66384028 66384486 66384028 ID=Msa0178950-mRNA-1:exon:21679;Parent=Msa0178950-mRNA-1
chr1_4 exon 66384581 66384729 66384581 ID=Msa0178950-mRNA-1:exon:21678;Parent=Msa0178950-mRNA-1
chr1_4 exon 66385812 66385861 66385812 ID=Msa0178950-mRNA-1:exon:21677;Parent=Msa0178950-mRNA-1
chr1_4 exon 66386122 66386183 66386122 ID=Msa0178950-mRNA-1:exon:21676;Parent=Msa0178950-mRNA-1
chr1_4 exon 66386278 66386337 66386278 ID=Msa0178950-mRNA-1:exon:21675;Parent=Msa0178950-mRNA-1
chr1_4 exon 66386459 66386515 66386459 ID=Msa0178950-mRNA-1:exon:21674;Parent=Msa0178950-mRNA-1
chr1_4 exon 66387245 66387327 66387245 ID=Msa0178950-mRNA-1:exon:21673;Parent=Msa0178950-mRNA-1
chr1_4 CDS 66387245 66387327 66387245 ID=Msa0178950-mRNA-1:cds;Parent=Msa0178950-mRNA-1
chr1_4 CDS 66386459 66386515 66386459 ID=Msa0178950-mRNA-1:cds;Parent=Msa0178950-mRNA-1
chr1_4 CDS 66386278 66386337 66386278 ID=Msa0178950-mRNA-1:cds;Parent=Msa0178950-mRNA-1
chr1_4 CDS 66386122 66386183 66386122 ID=Msa0178950-mRNA-1:cds;Parent=Msa0178950-mRNA-1
chr1_4 CDS 66385812 66385861 66385812 ID=Msa0178950-mRNA-1:cds;Parent=Msa0178950-mRNA-1
chr1_4 CDS 66384581 66384729 66384581 ID=Msa0178950-mRNA-1:cds;Parent=Msa0178950-mRNA-1
chr1_4 CDS 66384276 66384486 66384276 ID=Msa0178950-mRNA-1:cds;Parent=Msa0178950-mRNA-1
chr1_4 three_prime_UTR 66384028 66384275 66384028 ID=Msa0178950-mRNA-1:three_prime_utr;Parent=Msa0178950-mRNA-1
Gene Sequence

>Msa0178950

ATGGTGGGAGGATGGAGGCCGCCGGCTATGACGATTTTCTCCAGATCTGGACAGGGGTGGGGTGACGTTGGTGGTGGTCGAATAACCCTAATTCACACCAGCAGCAGAAAAGCCTCTCGTCCTCGTTTCTCTGTTCACAGGATTCCGCTTTCTTCATACTCTAAGTTTTATCTTATCGCAATGGGGAAGACAAATACAAGGTCAGATTCATTGGATTCAAAGTCGGATCGCAAGTTCGATAAGAAGCTTCAATTCTATACCAAGGTGAAAACTGCTGTTGCTTCCTTGACCGTCACAAAGTCTATTTCGAAGAATCAGAGAAAACATCAAAGGCGGCAGAAAAAATTGAAAGCATATAACCTCTCTTCGCTCTTGGATACACTTCCGGAGTTGAATGCACCACAGAAACCATGCAATGAGGATAACTTCAAGGTCAATTGTAAAACTAGACAGAAATTAGTATTGAAGGAAGGACAACGATTGTGCGAGCTTTTTAAGAATGATTCATTCCAAGTGAATCCCTTGGCTGCCATTCATCAACACTTAAGGAGCACACAACCAGAACCAGTAGCAGAGAAACAACAACCCAAGAAAAAAGCGAATGTAAATGGGTCAAAAAAGAGGAAGAAAAAATCAAAAGCTGGGGCTGGACTGAAGTCTATGGATATATAA
Protein sequence

>Msa0178950

MVGGWRPPAMTIFSRSGQGWGDVGGGRITLIHTSSRKASRPRFSVHRIPLSSYSKFYLIAMGKTNTRSDSLDSKSDRKFDKKLQFYTKVKTAVASLTVTKSISKNQRKHQRRQKKLKAYNLSSLLDTLPELNAPQKPCNEDNFKVNCKTRQKLVLKEGQRLCELFKNDSFQVNPLAAIHQHLRSTQPEPVAEKQQPKKKANVNGSKKRKKKSKAGAGLKSMDI*