AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0168800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0168800 MtrunA17_Chr1g0191671 94.692 584 11 9 1 571 1 577 0.0 1035
Msa0168800 MtrunA17_Chr2g0287751 68.354 79 25 0 1 79 1 79 1.64e-29 122
Msa0168800 MtrunA17_Chr1g0208301 66.667 78 23 1 1 75 1 78 3.95e-28 116
Msa0168800 MtrunA17_Chr0c22g0493881 64.103 78 28 0 1 78 1 78 7.73e-27 112
Msa0168800 MtrunA17_Chr4g0065651 64.103 78 28 0 1 78 1 78 9.34e-27 114
Msa0168800 MtrunA17_Chr4g0065621 64.103 78 28 0 1 78 1 78 4.54e-26 110
Msa0168800 MtrunA17_Chr2g0287741 54.945 91 39 1 12 102 17 105 6.49e-20 93.6
Msa0168800 MtrunA17_Chr7g0269081 57.143 70 30 0 5 74 17 86 1.34e-19 89.7
Msa0168800 MtrunA17_Chr3g0111541 52.113 71 34 0 3 73 5 75 1.17e-18 84.7
Msa0168800 MtrunA17_Chr4g0067291 55.224 67 30 0 7 73 71 137 4.35e-18 86.3
Msa0168800 MtrunA17_Chr7g0272571 54.545 66 30 0 9 74 32 97 2.87e-17 84.0
Msa0168800 MtrunA17_Chr1g0192131 52.174 69 32 1 7 74 12 80 1.27e-16 82.0
Msa0168800 MtrunA17_Chr8g0382721 60.656 61 24 0 14 74 33 93 4.34e-15 72.8
Msa0168800 MtrunA17_Chr2g0296001 54.412 68 31 0 7 74 70 137 4.26e-14 73.9
Msa0168800 MtrunA17_Chr3g0124411 54.545 66 30 0 9 74 29 94 4.51e-14 70.1
Msa0168800 MtrunA17_Chr2g0285561 39.474 76 45 1 4 78 2 77 9.05e-13 69.3
Msa0168800 MtrunA17_Chr6g0448861 41.791 67 38 1 9 74 29 95 1.17e-12 65.9
Msa0168800 MtrunA17_Chr1g0189221 43.243 74 42 0 1 74 44 117 4.44e-12 65.1
Msa0168800 MtrunA17_Chr5g0407821 40.278 72 43 0 3 74 16 87 6.31e-12 63.5
Msa0168800 MtrunA17_Chr4g0036931 41.096 73 42 1 3 74 21 93 7.91e-12 63.5
Msa0168800 MtrunA17_Chr7g0251491 40.541 74 43 1 2 74 40 113 9.29e-12 63.9
Msa0168800 MtrunA17_Chr5g0405811 42.647 68 38 1 8 74 27 94 1.58e-11 62.8
Msa0168800 MtrunA17_Chr4g0050571 44.286 70 38 1 10 79 101 169 2.99e-11 62.4
Msa0168800 MtrunA17_Chr1g0160201 39.394 66 40 0 9 74 24 89 3.33e-11 61.6
Msa0168800 MtrunA17_Chr3g0115421 37.349 83 49 1 14 93 30 112 3.62e-11 61.6
Msa0168800 MtrunA17_Chr2g0325601 41.026 78 45 1 14 91 6 82 4.56e-11 61.2
Msa0168800 MtrunA17_Chr1g0198341 42.647 68 39 0 7 74 1 68 5.04e-11 58.9
Msa0168800 MtrunA17_Chr3g0094841 47.761 67 34 1 9 74 115 181 5.92e-11 62.8
Msa0168800 MtrunA17_Chr5g0422891 40.323 62 37 0 14 75 29 90 8.99e-11 60.5
Msa0168800 MtrunA17_Chr6g0485081 41.176 68 39 1 7 74 62 128 9.35e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0168800 AT5G19090.2 63.366 101 36 1 1 101 1 100 1.67e-32 130
Msa0168800 AT5G19090.3 63.366 101 36 1 1 101 1 100 1.67e-32 130
Msa0168800 AT5G19090.5 63.366 101 36 1 1 101 1 100 5.43e-32 130
Msa0168800 AT5G19090.4 63.366 101 36 1 1 101 1 100 5.43e-32 130
Msa0168800 AT5G19090.1 63.366 101 36 1 1 101 1 100 5.43e-32 130
Msa0168800 AT3G05220.1 57.143 112 43 1 1 112 1 107 1.81e-29 123
Msa0168800 AT1G23000.1 47.656 128 54 4 6 132 9 124 6.91e-24 103
Msa0168800 AT1G23000.2 47.656 128 54 4 6 132 69 184 2.49e-23 103
Msa0168800 AT5G27690.1 47.059 85 45 0 7 91 27 111 2.76e-22 99.0
Msa0168800 AT5G37860.1 58.462 65 27 0 9 73 9 73 5.86e-19 87.4
Msa0168800 AT1G56210.1 58.730 63 26 0 10 72 40 102 2.20e-17 84.7
Msa0168800 AT1G06330.1 50.820 61 30 0 14 74 6 66 1.27e-13 68.9
Msa0168800 AT4G08570.1 47.059 68 35 1 8 74 24 91 5.30e-13 67.4
Msa0168800 AT1G22990.1 41.975 81 44 2 3 82 24 102 5.66e-12 64.3
Msa0168800 AT2G37390.1 47.692 65 33 1 10 74 181 244 7.26e-12 66.2
Msa0168800 AT2G37390.2 47.692 65 33 1 10 74 180 243 7.44e-12 66.2
Msa0168800 AT3G02960.1 41.892 74 42 1 3 75 119 192 1.44e-11 65.5
Msa0168800 AT5G02600.2 49.231 65 32 1 10 74 250 313 2.00e-11 65.9
Msa0168800 AT5G02600.1 49.231 65 32 1 10 74 250 313 2.00e-11 65.9
Msa0168800 AT1G22990.2 42.254 71 40 1 13 82 27 97 2.39e-11 62.4
Msa0168800 AT1G71050.1 40.909 66 39 0 9 74 27 92 3.24e-11 62.0
Msa0168800 AT4G23882.1 46.970 66 34 1 9 73 77 142 3.25e-11 64.7
Msa0168800 AT3G56891.1 45.902 61 33 0 14 74 20 80 4.52e-11 62.0
Msa0168800 AT5G17450.1 42.424 66 38 0 9 74 25 90 6.36e-11 61.2

Find 188 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCATTGCAACAAAATTTAA+TGG 0.134800 1_4:-51746498 None:intergenic
AGTAGTGGCTAGTTTGTTTC+AGG 0.151067 1_4:+51746446 Msa0168800:three_prime_UTR
AGGAAAATGTGCAAGATTTA+AGG 0.169787 1_4:+51746521 Msa0168800:three_prime_UTR
ATTATTACCATGTCCATTAT+TGG 0.170658 1_4:-51745926 None:intergenic
GAAGAAATCATACAATTTAA+TGG 0.241291 1_4:-51746698 None:intergenic
GGGCCTCCAGGCAATGGATT+TGG 0.269551 1_4:+51745274 Msa0168800:CDS
ACATCCATCAATGAACTATA+TGG 0.284194 1_4:+51746320 Msa0168800:CDS
GCCAAGGGCAATGCCAATAA+TGG 0.314955 1_4:+51745913 Msa0168800:CDS
GATGGAGGTAAGAAGAATAA+AGG 0.317363 1_4:+51745565 Msa0168800:CDS
TGCTGCTGCTGTTGCTGATA+AGG 0.327335 1_4:-51746153 None:intergenic
TGCTGTTGCTGATAAGGATT+TGG 0.330087 1_4:-51746147 None:intergenic
CTAGTTAAGTTTCTAGGGCT+TGG 0.332945 1_4:+51745682 Msa0168800:CDS
CAGGGAAACATGCTGAACTA+TGG 0.336084 1_4:+51744915 Msa0168800:CDS
AAGAAATCATACAATTTAAT+GGG 0.336801 1_4:-51746697 None:intergenic
CTGCCGGCATGCTCCTCATT+TGG 0.337899 1_4:-51746053 None:intergenic
GCCGGCAGTGCAAGGGTTAC+CGG 0.341648 1_4:+51746068 Msa0168800:CDS
AATGGACATGGTAATAATAA+TGG 0.358191 1_4:+51745931 Msa0168800:CDS
AGAAGAGAAGAAATAGTTGT+TGG 0.363777 1_4:-51744177 None:intergenic
ACAAGATATGATGAAAATTC+AGG 0.364742 1_4:+51744245 Msa0168800:CDS
GGCCTCCAGGCAATGGATTT+GGG 0.369230 1_4:+51745275 Msa0168800:CDS
GGGGAAAAGCAAAAGGGTGG+TGG 0.371759 1_4:+51745586 Msa0168800:CDS
CAGAATCAAAAGGGTCAAAA+AGG 0.373373 1_4:+51745049 Msa0168800:CDS
GGGGCAGCTGCGGCGATGAA+TGG 0.373441 1_4:+51746093 Msa0168800:CDS
ATGGTAATAATAATGGTAAT+GGG 0.379392 1_4:+51745938 Msa0168800:CDS
ACCATTACCTGGGATGTTCA+TGG 0.380238 1_4:-51745401 None:intergenic
AACATGTTTCTGAGATTTGT+TGG 0.389321 1_4:-51745128 None:intergenic
CCTCCTCCTTTACCATTACC+TGG 0.392465 1_4:-51745412 None:intergenic
ATGGAGGTAAGAAGAATAAA+GGG 0.400340 1_4:+51745566 Msa0168800:CDS
GAGGATGAAGAAGAGGAGTT+TGG 0.403036 1_4:+51745223 Msa0168800:CDS
TCTGATTCATTGCACCCATC+TGG 0.405299 1_4:-51745981 None:intergenic
GAAAAGCAAAAGGGTGGTGG+GGG 0.405813 1_4:+51745589 Msa0168800:CDS
AAAAGTGGTAAGGGTGGAAA+TGG 0.412152 1_4:+51745865 Msa0168800:CDS
CAGTGCAAGGGTTACCGGCC+GGG 0.416864 1_4:+51746073 Msa0168800:CDS
TCCATTATTGGCATTGCCCT+TGG 0.417589 1_4:-51745914 None:intergenic
GGAAAAGCAAAAGGGTGGTG+GGG 0.418059 1_4:+51745588 Msa0168800:CDS
GCCGGTAACCCTTGCACTGC+CGG 0.421629 1_4:-51746069 None:intergenic
CATGGTAATAATAATGGTAA+TGG 0.423098 1_4:+51745937 Msa0168800:CDS
TAGAGGCAAAAGGGTATTGT+TGG 0.425176 1_4:-51744124 None:intergenic
GGTGGAAATGGAAATGGAAA+TGG 0.432449 1_4:+51745877 Msa0168800:CDS
AATGGTCGTGGGCCACAGCT+AGG 0.432834 1_4:+51745343 Msa0168800:CDS
AGTGCAAGGGTTACCGGCCG+GGG 0.435816 1_4:+51746074 Msa0168800:CDS
GGCACCATGTCCTGCTCATA+AGG 0.437152 1_4:-51746606 None:intergenic
GAATAAAGGGGAAAAGCAAA+AGG 0.443691 1_4:+51745579 Msa0168800:CDS
GGTAATGGAGGTGGTAAGAA+AGG 0.449463 1_4:+51745535 Msa0168800:CDS
TATCCACAACATATGATGTA+TGG 0.452805 1_4:+51746285 Msa0168800:CDS
AGAAGTGATAAATTGATTGC+CGG 0.453817 1_4:-51744020 None:intergenic
TGCAAATGGATAATAACAAA+GGG 0.454184 1_4:+51745008 Msa0168800:CDS
TTATTGGCATTGCCCTTGGC+AGG 0.454606 1_4:-51745910 None:intergenic
AAACTGCTTCAGAAAATTGA+AGG 0.457319 1_4:+51744450 Msa0168800:CDS
ACCTATGCAAATGCAGATGA+AGG 0.459346 1_4:+51745480 Msa0168800:CDS
GTAATGGAGGTGGTAAGAAA+GGG 0.460661 1_4:+51745536 Msa0168800:CDS
ACATGTTTCTGAGATTTGTT+GGG 0.462682 1_4:-51745127 None:intergenic
GGGAAAAGCAAAAGGGTGGT+GGG 0.463087 1_4:+51745587 Msa0168800:CDS
TCTGGCCCATTGGACCCATA+TGG 0.464410 1_4:-51745963 None:intergenic
CAACAAGCCTCAGAATCAAA+AGG 0.465567 1_4:+51745039 Msa0168800:CDS
GGTAAGGGTGGAAATGGAAA+TGG 0.465972 1_4:+51745871 Msa0168800:CDS
TCACTGAAAACATGAGTGAT+TGG 0.466485 1_4:-51746378 None:intergenic
TGCTGCCCAAATCCATTGCC+TGG 0.470924 1_4:-51745280 None:intergenic
GTGGAAATGGAAATGGAAAT+GGG 0.471325 1_4:+51745878 Msa0168800:CDS
CAAGGGAAGGTGTTGGTGAC+AGG 0.475651 1_4:+51744851 Msa0168800:CDS
AGGGTATTGTTGGAATGCAT+TGG 0.476901 1_4:-51744114 None:intergenic
TCCTCATGGTAAAAGTGGTA+AGG 0.478115 1_4:+51745855 Msa0168800:CDS
TCAGTTTAAGAACATGCAAA+TGG 0.478310 1_4:+51744994 Msa0168800:CDS
AATGGTAAAGGAGGAGGTGG+TGG 0.479249 1_4:+51745418 Msa0168800:CDS
CCACAGCTAGGGCCTGGTGA+TGG 0.480756 1_4:+51745355 Msa0168800:CDS
AGGGAAACATGCTGAACTAT+GGG 0.482972 1_4:+51744916 Msa0168800:CDS
AATGGGTCAGATGGGTTCAA+TGG 0.483632 1_4:+51746029 Msa0168800:CDS
ATTGCACCCATCTGGCCCAT+TGG 0.483802 1_4:-51745973 None:intergenic
AAGGGTAAAAGTGGAAATTA+CGG 0.485308 1_4:+51745499 Msa0168800:CDS
ATCACTCCTCATGGTAAAAG+TGG 0.485671 1_4:+51745850 Msa0168800:CDS
ATTCATCGCCGCAGCTGCCC+CGG 0.485684 1_4:-51746091 None:intergenic
ATGAATAAGATGGGTCCAAT+GGG 0.487266 1_4:+51746012 Msa0168800:CDS
GCAGTGCAAGGGTTACCGGC+CGG 0.488422 1_4:+51746072 Msa0168800:CDS
GGCCATGGTCAAGGGCCTCC+AGG 0.488629 1_4:+51745262 Msa0168800:CDS
CATGGTCATGGCCATGGTCA+AGG 0.488816 1_4:+51745253 Msa0168800:CDS
GAGGAGTGATATCAAAGTCT+TGG 0.489452 1_4:-51745837 None:intergenic
CGTGGGCCACAGCTAGGGCC+TGG 0.491267 1_4:+51745349 Msa0168800:CDS
ATCGCCGCAGCTGCCCCGGC+CGG 0.491724 1_4:-51746087 None:intergenic
GATGGGTCCAATGGGTCAGA+TGG 0.493165 1_4:+51746020 Msa0168800:CDS
AACAACAGCAAAGGGAAAGA+AGG 0.496636 1_4:+51745775 Msa0168800:CDS
AGGAGCATGCCGGCAGTGCA+AGG 0.498242 1_4:+51746060 Msa0168800:CDS
AAAATATATCCGTACTACTT+AGG 0.499474 1_4:-51744064 None:intergenic
GTTACCGGCCGGGGCAGCTG+CGG 0.501977 1_4:+51746083 Msa0168800:CDS
ACCAATGCCATCACACCCTA+TGG 0.502702 1_4:+51746350 Msa0168800:CDS
GCCATGAACATCCCAGGTAA+TGG 0.503054 1_4:+51745400 Msa0168800:CDS
CCTGGGATGTTCATGGCAGC+AGG 0.506128 1_4:-51745394 None:intergenic
TGCCTGGAGGCCCTTGACCA+TGG 0.506822 1_4:-51745264 None:intergenic
GGTGATGGTATGATGATGAA+TGG 0.512661 1_4:+51745370 Msa0168800:CDS
GGTCAAGGGCCTCCAGGCAA+TGG 0.514555 1_4:+51745268 Msa0168800:CDS
CATCCATCAATGAACTATAT+GGG 0.516767 1_4:+51746321 Msa0168800:CDS
GATGGCAGCAATGAATAAGA+TGG 0.517515 1_4:+51746002 Msa0168800:CDS
ATTGAACCCATCTGACCCAT+TGG 0.518800 1_4:-51746027 None:intergenic
TCTGTAGAATTGTCTTCTCA+AGG 0.518947 1_4:+51744389 Msa0168800:intron
TATGGGTCCAATGGGCCAGA+TGG 0.519343 1_4:+51745966 Msa0168800:CDS
ATTTGCATGTTCTTAAACTG+AGG 0.523768 1_4:-51744992 None:intergenic
ATGAATGGTGGCTATTATCA+AGG 0.526298 1_4:+51746108 Msa0168800:CDS
TGTTTATTATCTTGCAGCAC+CGG 0.528186 1_4:+51744001 Msa0168800:five_prime_UTR
ATGGTCGTGGGCCACAGCTA+GGG 0.529327 1_4:+51745344 Msa0168800:CDS
GCATAATAACAAGATGATGA+TGG 0.529721 1_4:+51745309 Msa0168800:CDS
ATGGGTCCAATGGGCCAGAT+GGG 0.532006 1_4:+51745967 Msa0168800:CDS
CTGAATGTGCCTAAGTAGTA+CGG 0.535365 1_4:+51744055 Msa0168800:five_prime_UTR
ATATAGTTCATTGATGGATG+TGG 0.537347 1_4:-51746318 None:intergenic
TTGTGAAGGTTGTGAGCAGA+AGG 0.537353 1_4:+51744422 Msa0168800:CDS
CTCCTCCTTTACCATTACCT+GGG 0.537413 1_4:-51745411 None:intergenic
AACATCCCAGGTAATGGTAA+AGG 0.537780 1_4:+51745406 Msa0168800:CDS
ATAGCAACAACAACAGCAAA+GGG 0.537937 1_4:+51745767 Msa0168800:CDS
GGGTGTGATGGCATTGGTGG+CGG 0.538638 1_4:-51746345 None:intergenic
GCTATGATGCAGAATGGTCG+TGG 0.539141 1_4:+51745331 Msa0168800:CDS
AAAGGGAAAGAAGGTAAGAA+AGG 0.540026 1_4:+51745784 Msa0168800:CDS
CACGGACATGGTCATGGCCA+TGG 0.540291 1_4:+51745247 Msa0168800:CDS
GGTCCCATATAGTTCATTGA+TGG 0.543358 1_4:-51746324 None:intergenic
ATGCAGATGAAGGGTAAAAG+TGG 0.544101 1_4:+51745490 Msa0168800:CDS
ATAGGGTGTGATGGCATTGG+TGG 0.545253 1_4:-51746348 None:intergenic
GCCATAGGGTGTGATGGCAT+TGG 0.546440 1_4:-51746351 None:intergenic
GAGGAGTTTGGTCACGGACA+TGG 0.551855 1_4:+51745235 Msa0168800:CDS
ATAATAAACAGCTACAAACT+AGG 0.552187 1_4:-51743988 None:intergenic
TGGGTTCAATGGACCAAATG+AGG 0.553719 1_4:+51746040 Msa0168800:CDS
TAATGGAGGTGGTAAGAAAG+GGG 0.555785 1_4:+51745537 Msa0168800:CDS
ATGGTCATGGCCATGGTCAA+GGG 0.556259 1_4:+51745254 Msa0168800:CDS
CCCTTCATCTGCATTTGCAT+AGG 0.556545 1_4:-51745481 None:intergenic
AATGAATAAGATGGGTCCAA+TGG 0.557145 1_4:+51746011 Msa0168800:CDS
CCAGGTAATGGTAAAGGAGG+AGG 0.560490 1_4:+51745412 Msa0168800:CDS
TGGGAAAGGTGCTCCTGCCA+AGG 0.561931 1_4:+51745897 Msa0168800:CDS
GCAGCTGCGGCGATGAATGG+TGG 0.562369 1_4:+51746096 Msa0168800:CDS
AGGAGTGAATTAGGCACCAC+AGG 0.563041 1_4:-51746659 None:intergenic
GACTTTGATATCACTCCTCA+TGG 0.564860 1_4:+51745841 Msa0168800:CDS
CCTCCATACATCATATGTTG+TGG 0.564896 1_4:-51746288 None:intergenic
ACAACAACAACAGCAATACA+TGG 0.565187 1_4:+51746176 Msa0168800:CDS
GCACAGTTTCAAAATCCTAA+AGG 0.569465 1_4:+51745085 Msa0168800:CDS
CCTCATGGTAAAAGTGGTAA+GGG 0.569715 1_4:+51745856 Msa0168800:CDS
AAGTAATTAATTACTAGTAG+TGG 0.570079 1_4:+51746431 Msa0168800:exon
AACAAGCCTCAGAATCAAAA+GGG 0.570080 1_4:+51745040 Msa0168800:CDS
TTTGGTCACGGACATGGTCA+TGG 0.570828 1_4:+51745241 Msa0168800:CDS
GGTAATAATAATGGTAATGG+GGG 0.572168 1_4:+51745940 Msa0168800:CDS
GTAACGACAACCTTATGAGC+AGG 0.573000 1_4:+51746596 Msa0168800:three_prime_UTR
TGTGATACTCTGTCATTGAC+TGG 0.574552 1_4:-51746633 None:intergenic
ATGCAAATGGATAATAACAA+AGG 0.575532 1_4:+51745007 Msa0168800:CDS
AAGGTTAATATCCATTGTGA+AGG 0.575780 1_4:+51744408 Msa0168800:CDS
TGGTAATAATAATGGTAATG+GGG 0.579408 1_4:+51745939 Msa0168800:CDS
ATGGGTCCAATGGGTCAGAT+GGG 0.580478 1_4:+51746021 Msa0168800:CDS
GTGAATATAGATGCAGAACA+AGG 0.583193 1_4:+51744833 Msa0168800:CDS
GTGATGGTATGATGATGAAT+GGG 0.583869 1_4:+51745371 Msa0168800:CDS
GCAAATGGATAATAACAAAG+GGG 0.584331 1_4:+51745009 Msa0168800:CDS
TGAGTGATTGGATCTGCCAT+AGG 0.585575 1_4:-51746366 None:intergenic
CCTGCTGCCATGAACATCCC+AGG 0.587642 1_4:+51745394 Msa0168800:CDS
GGTAATGGTAAAGGAGGAGG+TGG 0.588777 1_4:+51745415 Msa0168800:CDS
AATAAAGGGGAAAAGCAAAA+GGG 0.595467 1_4:+51745580 Msa0168800:CDS
ATGGCAGCAATGAATAAGAT+GGG 0.598736 1_4:+51746003 Msa0168800:CDS
TATAGATGCAGAACAAGGGA+AGG 0.601009 1_4:+51744838 Msa0168800:CDS
GGATCTGCCATAGGGTGTGA+TGG 0.601407 1_4:-51746357 None:intergenic
CAACATATGATGTATGGAGG+AGG 0.605393 1_4:+51746291 Msa0168800:CDS
CCTATGCAAATGCAGATGAA+GGG 0.607502 1_4:+51745481 Msa0168800:CDS
TTCATCATCATACCATCACC+AGG 0.608129 1_4:-51745367 None:intergenic
AATAGCAACAACAACAGCAA+AGG 0.608297 1_4:+51745766 Msa0168800:CDS
AATGGAAATGGAAATGGGAA+AGG 0.608664 1_4:+51745883 Msa0168800:CDS
ATCCCAGGTAATGGTAAAGG+AGG 0.609225 1_4:+51745409 Msa0168800:CDS
ACTCTGTCATTGACTGGCAG+TGG 0.610829 1_4:-51746627 None:intergenic
ATCATCTTGTTATTATGCAT+AGG 0.612468 1_4:-51745304 None:intergenic
GGGAAAGGTGCTCCTGCCAA+GGG 0.613724 1_4:+51745898 Msa0168800:CDS
GGACCAAATGAGGAGCATGC+CGG 0.615075 1_4:+51746050 Msa0168800:CDS
CATGGTAAAAGTGGTAAGGG+TGG 0.615769 1_4:+51745859 Msa0168800:CDS
TGCAGAACAAGGGAAGGTGT+TGG 0.615855 1_4:+51744844 Msa0168800:CDS
GGAGCATGCCGGCAGTGCAA+GGG 0.616628 1_4:+51746061 Msa0168800:CDS
GAAGAAGAGGAGTTTGGTCA+CGG 0.617819 1_4:+51745229 Msa0168800:CDS
GATGGGTGCAATGAATCAGA+TGG 0.620881 1_4:+51745984 Msa0168800:CDS
GGCAATGCCAATAATGGACA+TGG 0.622193 1_4:+51745919 Msa0168800:CDS
AATGGAGGTGGTAAGAAAGG+GGG 0.629262 1_4:+51745538 Msa0168800:CDS
CCATCACCAGGCCCTAGCTG+TGG 0.636272 1_4:-51745355 None:intergenic
AAAGGGGAAAAGCAAAAGGG+TGG 0.640635 1_4:+51745583 Msa0168800:CDS
TCATAAGGTTGTCGTTACAC+AGG 0.641578 1_4:-51746591 None:intergenic
CCACAACATATGATGTATGG+AGG 0.641920 1_4:+51746288 Msa0168800:CDS
CAGCAGCAACAAATGCAGCA+AGG 0.643167 1_4:+51746246 Msa0168800:CDS
ACAACCTTATGAGCAGGACA+TGG 0.650595 1_4:+51746602 Msa0168800:three_prime_UTR
GAGTGATTGGATCTGCCATA+GGG 0.655708 1_4:-51746365 None:intergenic
ATATGATGTATGGAGGAGGA+AGG 0.656963 1_4:+51746295 Msa0168800:CDS
AAACATGCTGAACTATGGGG+TGG 0.661564 1_4:+51744920 Msa0168800:CDS
ATGATGGCTATGATGCAGAA+TGG 0.662536 1_4:+51745325 Msa0168800:CDS
TGAATATAGATGCAGAACAA+GGG 0.662709 1_4:+51744834 Msa0168800:CDS
TGGAGGTAAGAAGAATAAAG+GGG 0.662757 1_4:+51745567 Msa0168800:CDS
CAAATGGATAATAACAAAGG+GGG 0.663697 1_4:+51745010 Msa0168800:CDS
CAGTTTCAAAATCCTAAAGG+AGG 0.665835 1_4:+51745088 Msa0168800:CDS
TCTGCTCACAACCTTCACAA+TGG 0.671031 1_4:-51744419 None:intergenic
GAGGAATTTGATGAGAGTGA+TGG 0.688249 1_4:+51745169 Msa0168800:CDS
GGGAAACATGCTGAACTATG+GGG 0.688345 1_4:+51744917 Msa0168800:CDS
TGCCCAAATCCATTGCCTGG+AGG 0.700160 1_4:-51745277 None:intergenic
CTATGATGCAGAATGGTCGT+GGG 0.701836 1_4:+51745332 Msa0168800:CDS
TAAAAGTGGAAATTACGGTG+AGG 0.728158 1_4:+51745504 Msa0168800:CDS
ACAGAGTATCACATGTCCTG+TGG 0.743374 1_4:+51746643 Msa0168800:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATTTTGTCTTTTTTTT+TGG + chr1_4:51746472-51746491 Msa0168800:three_prime_UTR 10.0%
!!! ATTATTTTGTCTTTTTTTTT+GGG + chr1_4:51746473-51746492 Msa0168800:three_prime_UTR 10.0%
!!! AGATATTTTTTTTTCTATAG+AGG - chr1_4:51744144-51744163 None:intergenic 15.0%
!!! TGAACTTTTTTATGTTTATA+TGG + chr1_4:51744693-51744712 Msa0168800:intron 15.0%
!!! TTATTTTGTCTTTTTTTTTG+GGG + chr1_4:51746474-51746493 Msa0168800:three_prime_UTR 15.0%
!! AAGAAATCATACAATTTAAT+GGG - chr1_4:51746700-51746719 None:intergenic 15.0%
!! AAAAATAAGTAAGAAGAACA+AGG - chr1_4:51744276-51744295 None:intergenic 20.0%
!! AAACAAAAACTTTACTATGT+CGG + chr1_4:51744582-51744601 Msa0168800:intron 20.0%
!! AAAAAACATGAAATTAAGGA+GGG - chr1_4:51744632-51744651 None:intergenic 20.0%
!! AAAAATGGTAAAGGTAAAAA+CGG + chr1_4:51745646-51745665 Msa0168800:CDS 20.0%
!! ATGGTAATAATAATGGTAAT+GGG + chr1_4:51745938-51745957 Msa0168800:CDS 20.0%
!! AAGTAATTAATTACTAGTAG+TGG + chr1_4:51746431-51746450 Msa0168800:exon 20.0%
!! GAAGAAATCATACAATTTAA+TGG - chr1_4:51746701-51746720 None:intergenic 20.0%
! ATAATAAACAGCTACAAACT+AGG - chr1_4:51743991-51744010 None:intergenic 25.0%
! AAAATATATCCGTACTACTT+AGG - chr1_4:51744067-51744086 None:intergenic 25.0%
!!! TTTTTTCTATAGAGGCAAAA+GGG - chr1_4:51744136-51744155 None:intergenic 25.0%
!!! TTTTTTTCTATAGAGGCAAA+AGG - chr1_4:51744137-51744156 None:intergenic 25.0%
! ACAAGATATGATGAAAATTC+AGG + chr1_4:51744245-51744264 Msa0168800:CDS 25.0%
! AATCTGTACTTATGTTTAAC+TGG + chr1_4:51744346-51744365 Msa0168800:intron 25.0%
!!! ACTTATGTTTAACTGGTTTT+TGG + chr1_4:51744353-51744372 Msa0168800:intron 25.0%
! ACATGTTAACTTTACTATGT+CGG + chr1_4:51744541-51744560 Msa0168800:intron 25.0%
! GAAAAAACATGAAATTAAGG+AGG - chr1_4:51744633-51744652 None:intergenic 25.0%
! AGGGAAAAAACATGAAATTA+AGG - chr1_4:51744636-51744655 None:intergenic 25.0%
! TCACAAAAACATCATAAAGT+TGG - chr1_4:51744676-51744695 None:intergenic 25.0%
! ATGCAAATGGATAATAACAA+AGG + chr1_4:51745007-51745026 Msa0168800:CDS 25.0%
! TGCAAATGGATAATAACAAA+GGG + chr1_4:51745008-51745027 Msa0168800:CDS 25.0%
!!! TGTTAATTTTGATTTGTCTG+AGG + chr1_4:51745147-51745166 Msa0168800:CDS 25.0%
!!! ATCATCTTGTTATTATGCAT+AGG - chr1_4:51745307-51745326 None:intergenic 25.0%
! AATTATGCAAAAGCCAAAAA+AGG + chr1_4:51745442-51745461 Msa0168800:CDS 25.0%
!!! ATCCATAATATCTCCTTTTT+TGG - chr1_4:51745458-51745477 None:intergenic 25.0%
! AATAGTAGCAAGAAGAAAAA+TGG + chr1_4:51745631-51745650 Msa0168800:CDS 25.0%
!!! GTTTTCTAGTTAAGTTTCTA+GGG + chr1_4:51745677-51745696 Msa0168800:CDS 25.0%
! ATTATTACCATGTCCATTAT+TGG - chr1_4:51745929-51745948 None:intergenic 25.0%
! AATGGACATGGTAATAATAA+TGG + chr1_4:51745931-51745950 Msa0168800:CDS 25.0%
! CATGGTAATAATAATGGTAA+TGG + chr1_4:51745937-51745956 Msa0168800:CDS 25.0%
!! TGGTAATAATAATGGTAATG+GGG + chr1_4:51745939-51745958 Msa0168800:CDS 25.0%
!! TCCATTAAATTTTGTTGCAA+TGG + chr1_4:51746497-51746516 Msa0168800:three_prime_UTR 25.0%
! ACCATTGCAACAAAATTTAA+TGG - chr1_4:51746501-51746520 None:intergenic 25.0%
!! TTAAATTTTGTTGCAATGGT+AGG + chr1_4:51746501-51746520 Msa0168800:three_prime_UTR 25.0%
AGAAGTGATAAATTGATTGC+CGG - chr1_4:51744023-51744042 None:intergenic 30.0%
AGAAGAGAAGAAATAGTTGT+TGG - chr1_4:51744180-51744199 None:intergenic 30.0%
GTACAGATTCAACTTTAACT+AGG - chr1_4:51744335-51744354 None:intergenic 30.0%
AAGGTTAATATCCATTGTGA+AGG + chr1_4:51744408-51744427 Msa0168800:CDS 30.0%
AAACTGCTTCAGAAAATTGA+AGG + chr1_4:51744450-51744469 Msa0168800:CDS 30.0%
AAGAAAAGAAGAAAGAGACA+TGG - chr1_4:51744486-51744505 None:intergenic 30.0%
CATCATAAAGTTGGAAACTT+TGG - chr1_4:51744667-51744686 None:intergenic 30.0%
TGAATATAGATGCAGAACAA+GGG + chr1_4:51744834-51744853 Msa0168800:CDS 30.0%
TCAAAAAGCTGAAAAGTTCA+GGG + chr1_4:51744897-51744916 Msa0168800:CDS 30.0%
ATTTGCATGTTCTTAAACTG+AGG - chr1_4:51744995-51745014 None:intergenic 30.0%
TCAGTTTAAGAACATGCAAA+TGG + chr1_4:51744994-51745013 Msa0168800:CDS 30.0%
GCAAATGGATAATAACAAAG+GGG + chr1_4:51745009-51745028 Msa0168800:CDS 30.0%
CAAATGGATAATAACAAAGG+GGG + chr1_4:51745010-51745029 Msa0168800:CDS 30.0%
! TTCATATCTTTTCCTCCTTT+AGG - chr1_4:51745103-51745122 None:intergenic 30.0%
!! ACATGTTTCTGAGATTTGTT+GGG - chr1_4:51745130-51745149 None:intergenic 30.0%
!! AACATGTTTCTGAGATTTGT+TGG - chr1_4:51745131-51745150 None:intergenic 30.0%
!!! TAATTTTGATTTGTCTGAGG+AGG + chr1_4:51745150-51745169 Msa0168800:CDS 30.0%
GCATAATAACAAGATGATGA+TGG + chr1_4:51745309-51745328 Msa0168800:CDS 30.0%
AGCCAAAAAAGGAGATATTA+TGG + chr1_4:51745453-51745472 Msa0168800:CDS 30.0%
AAGGGTAAAAGTGGAAATTA+CGG + chr1_4:51745499-51745518 Msa0168800:CDS 30.0%
AATTACGGTGAGGTAAAAAA+TGG + chr1_4:51745514-51745533 Msa0168800:CDS 30.0%
ATGGAGGTAAGAAGAATAAA+GGG + chr1_4:51745566-51745585 Msa0168800:CDS 30.0%
AATAAAGGGGAAAAGCAAAA+GGG + chr1_4:51745580-51745599 Msa0168800:CDS 30.0%
AGCAAGAAGAAAAATGGTAA+AGG + chr1_4:51745637-51745656 Msa0168800:CDS 30.0%
!!! GGTTTTCTAGTTAAGTTTCT+AGG + chr1_4:51745676-51745695 Msa0168800:CDS 30.0%
CTTGGAAAAAAGAGCAAAAA+GGG + chr1_4:51745700-51745719 Msa0168800:CDS 30.0%
!!! TCCATTGTTTTTGTTCTTGT+CGG - chr1_4:51745737-51745756 None:intergenic 30.0%
AGGTAAGAAAGGTGAAAAAT+TGG + chr1_4:51745795-51745814 Msa0168800:CDS 30.0%
!! GGTAATAATAATGGTAATGG+GGG + chr1_4:51745940-51745959 Msa0168800:CDS 30.0%
TATCCACAACATATGATGTA+TGG + chr1_4:51746285-51746304 Msa0168800:CDS 30.0%
! ATATAGTTCATTGATGGATG+TGG - chr1_4:51746321-51746340 None:intergenic 30.0%
ACATCCATCAATGAACTATA+TGG + chr1_4:51746320-51746339 Msa0168800:CDS 30.0%
CATCCATCAATGAACTATAT+GGG + chr1_4:51746321-51746340 Msa0168800:CDS 30.0%
AGGAAAATGTGCAAGATTTA+AGG + chr1_4:51746521-51746540 Msa0168800:three_prime_UTR 30.0%
!!! TAAGTTTTGAGGAGTGAATT+AGG - chr1_4:51746671-51746690 None:intergenic 30.0%
!! ATGGGTTAAGATAAGTTTTG+AGG - chr1_4:51746682-51746701 None:intergenic 30.0%
TGTTTATTATCTTGCAGCAC+CGG + chr1_4:51744001-51744020 Msa0168800:five_prime_UTR 35.0%
TCTGTAGAATTGTCTTCTCA+AGG + chr1_4:51744389-51744408 Msa0168800:intron 35.0%
TAAGGAGGGTTTAGAAACAT+GGG - chr1_4:51744618-51744637 None:intergenic 35.0%
TTAAGGAGGGTTTAGAAACA+TGG - chr1_4:51744619-51744638 None:intergenic 35.0%
GGAAACTTTGGATGAATGAA+GGG - chr1_4:51744655-51744674 None:intergenic 35.0%
TGGAAACTTTGGATGAATGA+AGG - chr1_4:51744656-51744675 None:intergenic 35.0%
GTGAATATAGATGCAGAACA+AGG + chr1_4:51744833-51744852 Msa0168800:CDS 35.0%
! CTGAACTTTTCAGCTTTTTG+AGG - chr1_4:51744898-51744917 None:intergenic 35.0%
CTCAAAAAGCTGAAAAGTTC+AGG + chr1_4:51744896-51744915 Msa0168800:CDS 35.0%
AACAAGCCTCAGAATCAAAA+GGG + chr1_4:51745040-51745059 Msa0168800:CDS 35.0%
!! TTTTGACCCTTTTGATTCTG+AGG - chr1_4:51745049-51745068 None:intergenic 35.0%
CAGAATCAAAAGGGTCAAAA+AGG + chr1_4:51745049-51745068 Msa0168800:CDS 35.0%
AATCAAAAGGGTCAAAAAGG+TGG + chr1_4:51745052-51745071 Msa0168800:CDS 35.0%
GCACAGTTTCAAAATCCTAA+AGG + chr1_4:51745085-51745104 Msa0168800:CDS 35.0%
CAGTTTCAAAATCCTAAAGG+AGG + chr1_4:51745088-51745107 Msa0168800:CDS 35.0%
!! CTATGACGATGATTTTGATG+AGG + chr1_4:51745204-51745223 Msa0168800:CDS 35.0%
! TTTTGATGAGGATGAAGAAG+AGG + chr1_4:51745216-51745235 Msa0168800:CDS 35.0%
! GTGATGGTATGATGATGAAT+GGG + chr1_4:51745371-51745390 Msa0168800:CDS 35.0%
TAAAAGTGGAAATTACGGTG+AGG + chr1_4:51745504-51745523 Msa0168800:CDS 35.0%
GGTGAGGTAAAAAATGGTAA+TGG + chr1_4:51745520-51745539 Msa0168800:CDS 35.0%
!! GAGGTAAAAAATGGTAATGG+AGG + chr1_4:51745523-51745542 Msa0168800:CDS 35.0%
!! GTAAAAAATGGTAATGGAGG+TGG + chr1_4:51745526-51745545 Msa0168800:CDS 35.0%
GATGGAGGTAAGAAGAATAA+AGG + chr1_4:51745565-51745584 Msa0168800:CDS 35.0%
TGGAGGTAAGAAGAATAAAG+GGG + chr1_4:51745567-51745586 Msa0168800:CDS 35.0%
GAATAAAGGGGAAAAGCAAA+AGG + chr1_4:51745579-51745598 Msa0168800:CDS 35.0%
AATGGTAAAGGTAAAAACGG+TGG + chr1_4:51745649-51745668 Msa0168800:CDS 35.0%
GCTTGGAAAAAAGAGCAAAA+AGG + chr1_4:51745699-51745718 Msa0168800:CDS 35.0%
GACAAGAACAAAAACAATGG+AGG + chr1_4:51745736-51745755 Msa0168800:CDS 35.0%
AAGAACAAAAACAATGGAGG+AGG + chr1_4:51745739-51745758 Msa0168800:CDS 35.0%
AATAGCAACAACAACAGCAA+AGG + chr1_4:51745766-51745785 Msa0168800:CDS 35.0%
ATAGCAACAACAACAGCAAA+GGG + chr1_4:51745767-51745786 Msa0168800:CDS 35.0%
AAAGGGAAAGAAGGTAAGAA+AGG + chr1_4:51745784-51745803 Msa0168800:CDS 35.0%
GTGGAAATGGAAATGGAAAT+GGG + chr1_4:51745878-51745897 Msa0168800:CDS 35.0%
AATGGAAATGGAAATGGGAA+AGG + chr1_4:51745883-51745902 Msa0168800:CDS 35.0%
ATGGCAGCAATGAATAAGAT+GGG + chr1_4:51746003-51746022 Msa0168800:CDS 35.0%
AATGAATAAGATGGGTCCAA+TGG + chr1_4:51746011-51746030 Msa0168800:CDS 35.0%
ATGAATAAGATGGGTCCAAT+GGG + chr1_4:51746012-51746031 Msa0168800:CDS 35.0%
ATGAATGGTGGCTATTATCA+AGG + chr1_4:51746108-51746127 Msa0168800:CDS 35.0%
ACAACAACAACAGCAATACA+TGG + chr1_4:51746176-51746195 Msa0168800:CDS 35.0%
TCACTGAAAACATGAGTGAT+TGG - chr1_4:51746381-51746400 None:intergenic 35.0%
CTGAATGTGCCTAAGTAGTA+CGG + chr1_4:51744055-51744074 Msa0168800:five_prime_UTR 40.0%
!! AGGGTATTGTTGGAATGCAT+TGG - chr1_4:51744117-51744136 None:intergenic 40.0%
!! TAGAGGCAAAAGGGTATTGT+TGG - chr1_4:51744127-51744146 None:intergenic 40.0%
! TGTTGTTGTTGTTGTGATGC+AGG + chr1_4:51744800-51744819 Msa0168800:intron 40.0%
! GTTGTTGTTGTTGTGATGCA+GGG + chr1_4:51744801-51744820 Msa0168800:intron 40.0%
! TTGTTGTTGTTGTGATGCAG+GGG + chr1_4:51744802-51744821 Msa0168800:intron 40.0%
TATAGATGCAGAACAAGGGA+AGG + chr1_4:51744838-51744857 Msa0168800:CDS 40.0%
AGGGAAACATGCTGAACTAT+GGG + chr1_4:51744916-51744935 Msa0168800:CDS 40.0%
CAACAAGCCTCAGAATCAAA+AGG + chr1_4:51745039-51745058 Msa0168800:CDS 40.0%
GAGGAATTTGATGAGAGTGA+TGG + chr1_4:51745169-51745188 Msa0168800:CDS 40.0%
ATGATGGCTATGATGCAGAA+TGG + chr1_4:51745325-51745344 Msa0168800:CDS 40.0%
TTCATCATCATACCATCACC+AGG - chr1_4:51745370-51745389 None:intergenic 40.0%
! GGTGATGGTATGATGATGAA+TGG + chr1_4:51745370-51745389 Msa0168800:CDS 40.0%
! AACATCCCAGGTAATGGTAA+AGG + chr1_4:51745406-51745425 Msa0168800:CDS 40.0%
ACCTATGCAAATGCAGATGA+AGG + chr1_4:51745480-51745499 Msa0168800:CDS 40.0%
CCTATGCAAATGCAGATGAA+GGG + chr1_4:51745481-51745500 Msa0168800:CDS 40.0%
ATGCAGATGAAGGGTAAAAG+TGG + chr1_4:51745490-51745509 Msa0168800:CDS 40.0%
! GTAATGGAGGTGGTAAGAAA+GGG + chr1_4:51745536-51745555 Msa0168800:CDS 40.0%
TAATGGAGGTGGTAAGAAAG+GGG + chr1_4:51745537-51745556 Msa0168800:CDS 40.0%
!! CTAGTTAAGTTTCTAGGGCT+TGG + chr1_4:51745682-51745701 Msa0168800:CDS 40.0%
GGAAAAAAGAGCAAAAAGGG+AGG + chr1_4:51745703-51745722 Msa0168800:CDS 40.0%
AAAAAGAGCAAAAAGGGAGG+AGG + chr1_4:51745706-51745725 Msa0168800:CDS 40.0%
GCCGACAAGAACAAAAACAA+TGG + chr1_4:51745733-51745752 Msa0168800:CDS 40.0%
AACAAAAACAATGGAGGAGG+TGG + chr1_4:51745742-51745761 Msa0168800:CDS 40.0%
AACAACAGCAAAGGGAAAGA+AGG + chr1_4:51745775-51745794 Msa0168800:CDS 40.0%
GAGGAGTGATATCAAAGTCT+TGG - chr1_4:51745840-51745859 None:intergenic 40.0%
GACTTTGATATCACTCCTCA+TGG + chr1_4:51745841-51745860 Msa0168800:CDS 40.0%
ATCACTCCTCATGGTAAAAG+TGG + chr1_4:51745850-51745869 Msa0168800:CDS 40.0%
TCCTCATGGTAAAAGTGGTA+AGG + chr1_4:51745855-51745874 Msa0168800:CDS 40.0%
CCTCATGGTAAAAGTGGTAA+GGG + chr1_4:51745856-51745875 Msa0168800:CDS 40.0%
AAAAGTGGTAAGGGTGGAAA+TGG + chr1_4:51745865-51745884 Msa0168800:CDS 40.0%
GGTGGAAATGGAAATGGAAA+TGG + chr1_4:51745877-51745896 Msa0168800:CDS 40.0%
GATGGCAGCAATGAATAAGA+TGG + chr1_4:51746002-51746021 Msa0168800:CDS 40.0%
TGCTGTTGCTGATAAGGATT+TGG - chr1_4:51746150-51746169 None:intergenic 40.0%
CCTCCATACATCATATGTTG+TGG - chr1_4:51746291-51746310 None:intergenic 40.0%
CCACAACATATGATGTATGG+AGG + chr1_4:51746288-51746307 Msa0168800:CDS 40.0%
CAACATATGATGTATGGAGG+AGG + chr1_4:51746291-51746310 Msa0168800:CDS 40.0%
ATATGATGTATGGAGGAGGA+AGG + chr1_4:51746295-51746314 Msa0168800:CDS 40.0%
GGTCCCATATAGTTCATTGA+TGG - chr1_4:51746327-51746346 None:intergenic 40.0%
!! AGTAGTGGCTAGTTTGTTTC+AGG + chr1_4:51746446-51746465 Msa0168800:three_prime_UTR 40.0%
TCATAAGGTTGTCGTTACAC+AGG - chr1_4:51746594-51746613 None:intergenic 40.0%
TGTGATACTCTGTCATTGAC+TGG - chr1_4:51746636-51746655 None:intergenic 40.0%
TCTGCTCACAACCTTCACAA+TGG - chr1_4:51744422-51744441 None:intergenic 45.0%
TTGTGAAGGTTGTGAGCAGA+AGG + chr1_4:51744422-51744441 Msa0168800:CDS 45.0%
!! AGCTTTTTGAGGAGCTTTGC+TGG - chr1_4:51744887-51744906 None:intergenic 45.0%
CAGGGAAACATGCTGAACTA+TGG + chr1_4:51744915-51744934 Msa0168800:CDS 45.0%
GGGAAACATGCTGAACTATG+GGG + chr1_4:51744917-51744936 Msa0168800:CDS 45.0%
AAACATGCTGAACTATGGGG+TGG + chr1_4:51744920-51744939 Msa0168800:CDS 45.0%
GAGGATGAAGAAGAGGAGTT+TGG + chr1_4:51745223-51745242 Msa0168800:CDS 45.0%
GAAGAAGAGGAGTTTGGTCA+CGG + chr1_4:51745229-51745248 Msa0168800:CDS 45.0%
CTATGATGCAGAATGGTCGT+GGG + chr1_4:51745332-51745351 Msa0168800:CDS 45.0%
ACCATTACCTGGGATGTTCA+TGG - chr1_4:51745404-51745423 None:intergenic 45.0%
! ATCCCAGGTAATGGTAAAGG+AGG + chr1_4:51745409-51745428 Msa0168800:CDS 45.0%
CTCCTCCTTTACCATTACCT+GGG - chr1_4:51745414-51745433 None:intergenic 45.0%
CCCTTCATCTGCATTTGCAT+AGG - chr1_4:51745484-51745503 None:intergenic 45.0%
!! GGTAATGGAGGTGGTAAGAA+AGG + chr1_4:51745535-51745554 Msa0168800:CDS 45.0%
AATGGAGGTGGTAAGAAAGG+GGG + chr1_4:51745538-51745557 Msa0168800:CDS 45.0%
AAAGGGGAAAAGCAAAAGGG+TGG + chr1_4:51745583-51745602 Msa0168800:CDS 45.0%
GGTAAAGGTAAAAACGGTGG+TGG + chr1_4:51745652-51745671 Msa0168800:CDS 45.0%
! AAAGGTAAAAACGGTGGTGG+TGG + chr1_4:51745655-51745674 Msa0168800:CDS 45.0%
!! CCCTTACCACTTTTACCATG+AGG - chr1_4:51745859-51745878 None:intergenic 45.0%
CATGGTAAAAGTGGTAAGGG+TGG + chr1_4:51745859-51745878 Msa0168800:CDS 45.0%
GGTAAGGGTGGAAATGGAAA+TGG + chr1_4:51745871-51745890 Msa0168800:CDS 45.0%
TCCATTATTGGCATTGCCCT+TGG - chr1_4:51745917-51745936 None:intergenic 45.0%
!! GGCAATGCCAATAATGGACA+TGG + chr1_4:51745919-51745938 Msa0168800:CDS 45.0%
!! TAATGGTAATGGGGGCCATA+TGG + chr1_4:51745948-51745967 Msa0168800:CDS 45.0%
!! AATGGTAATGGGGGCCATAT+GGG + chr1_4:51745949-51745968 Msa0168800:CDS 45.0%
TCTGATTCATTGCACCCATC+TGG - chr1_4:51745984-51746003 None:intergenic 45.0%
GATGGGTGCAATGAATCAGA+TGG + chr1_4:51745984-51746003 Msa0168800:CDS 45.0%
ATTGAACCCATCTGACCCAT+TGG - chr1_4:51746030-51746049 None:intergenic 45.0%
! AATGGGTCAGATGGGTTCAA+TGG + chr1_4:51746029-51746048 Msa0168800:CDS 45.0%
TGGGTTCAATGGACCAAATG+AGG + chr1_4:51746040-51746059 Msa0168800:CDS 45.0%
GAGTGATTGGATCTGCCATA+GGG - chr1_4:51746368-51746387 None:intergenic 45.0%
TGAGTGATTGGATCTGCCAT+AGG - chr1_4:51746369-51746388 None:intergenic 45.0%
GTAACGACAACCTTATGAGC+AGG + chr1_4:51746596-51746615 Msa0168800:three_prime_UTR 45.0%
ACAACCTTATGAGCAGGACA+TGG + chr1_4:51746602-51746621 Msa0168800:three_prime_UTR 45.0%
! ACAGAGTATCACATGTCCTG+TGG + chr1_4:51746643-51746662 Msa0168800:three_prime_UTR 45.0%
! TGCAGAACAAGGGAAGGTGT+TGG + chr1_4:51744844-51744863 Msa0168800:CDS 50.0%
TTTGGTCACGGACATGGTCA+TGG + chr1_4:51745241-51745260 Msa0168800:CDS 50.0%
ATGGTCATGGCCATGGTCAA+GGG + chr1_4:51745254-51745273 Msa0168800:CDS 50.0%
GCTATGATGCAGAATGGTCG+TGG + chr1_4:51745331-51745350 Msa0168800:CDS 50.0%
GCCATGAACATCCCAGGTAA+TGG + chr1_4:51745400-51745419 Msa0168800:CDS 50.0%
CCTCCTCCTTTACCATTACC+TGG - chr1_4:51745415-51745434 None:intergenic 50.0%
! CCAGGTAATGGTAAAGGAGG+AGG + chr1_4:51745412-51745431 Msa0168800:CDS 50.0%
! GGTAATGGTAAAGGAGGAGG+TGG + chr1_4:51745415-51745434 Msa0168800:CDS 50.0%
AATGGTAAAGGAGGAGGTGG+TGG + chr1_4:51745418-51745437 Msa0168800:CDS 50.0%
GGTAAGAAAGGGGGTGATGA+TGG + chr1_4:51745547-51745566 Msa0168800:CDS 50.0%
!! AAGAAAGGGGGTGATGATGG+AGG + chr1_4:51745550-51745569 Msa0168800:CDS 50.0%
GGGAAAAGCAAAAGGGTGGT+GGG + chr1_4:51745587-51745606 Msa0168800:CDS 50.0%
GGAAAAGCAAAAGGGTGGTG+GGG + chr1_4:51745588-51745607 Msa0168800:CDS 50.0%
! GAAAAGCAAAAGGGTGGTGG+GGG + chr1_4:51745589-51745608 Msa0168800:CDS 50.0%
TTATTGGCATTGCCCTTGGC+AGG - chr1_4:51745913-51745932 None:intergenic 50.0%
GCCAAGGGCAATGCCAATAA+TGG + chr1_4:51745913-51745932 Msa0168800:CDS 50.0%
ATGGGTCCAATGGGTCAGAT+GGG + chr1_4:51746021-51746040 Msa0168800:CDS 50.0%
TGCTGCTGCTGTTGCTGATA+AGG - chr1_4:51746156-51746175 None:intergenic 50.0%
CAGCAGCAACAAATGCAGCA+AGG + chr1_4:51746219-51746238 Msa0168800:CDS 50.0%
! ATAGGGTGTGATGGCATTGG+TGG - chr1_4:51746351-51746370 None:intergenic 50.0%
ACCAATGCCATCACACCCTA+TGG + chr1_4:51746350-51746369 Msa0168800:CDS 50.0%
! ACTCTGTCATTGACTGGCAG+TGG - chr1_4:51746630-51746649 None:intergenic 50.0%
AGGAGTGAATTAGGCACCAC+AGG - chr1_4:51746662-51746681 None:intergenic 50.0%
!! CAAGGGAAGGTGTTGGTGAC+AGG + chr1_4:51744851-51744870 Msa0168800:CDS 55.0%
GAGGAGTTTGGTCACGGACA+TGG + chr1_4:51745235-51745254 Msa0168800:CDS 55.0%
CATGGTCATGGCCATGGTCA+AGG + chr1_4:51745253-51745272 Msa0168800:CDS 55.0%
! GGCCTCCAGGCAATGGATTT+GGG + chr1_4:51745275-51745294 Msa0168800:CDS 55.0%
TGCCCAAATCCATTGCCTGG+AGG - chr1_4:51745280-51745299 None:intergenic 55.0%
TGCTGCCCAAATCCATTGCC+TGG - chr1_4:51745283-51745302 None:intergenic 55.0%
GGGGAAAAGCAAAAGGGTGG+TGG + chr1_4:51745586-51745605 Msa0168800:CDS 55.0%
!! AAAAGGGTGGTGGGGGAGAT+TGG + chr1_4:51745596-51745615 Msa0168800:CDS 55.0%
!! AAAGGGTGGTGGGGGAGATT+GGG + chr1_4:51745597-51745616 Msa0168800:CDS 55.0%
TCTGGCCCATTGGACCCATA+TGG - chr1_4:51745966-51745985 None:intergenic 55.0%
TATGGGTCCAATGGGCCAGA+TGG + chr1_4:51745966-51745985 Msa0168800:CDS 55.0%
ATGGGTCCAATGGGCCAGAT+GGG + chr1_4:51745967-51745986 Msa0168800:CDS 55.0%
ATTGCACCCATCTGGCCCAT+TGG - chr1_4:51745976-51745995 None:intergenic 55.0%
GATGGGTCCAATGGGTCAGA+TGG + chr1_4:51746020-51746039 Msa0168800:CDS 55.0%
! GGACCAAATGAGGAGCATGC+CGG + chr1_4:51746050-51746069 Msa0168800:CDS 55.0%
GCCATAGGGTGTGATGGCAT+TGG - chr1_4:51746354-51746373 None:intergenic 55.0%
GGATCTGCCATAGGGTGTGA+TGG - chr1_4:51746360-51746379 None:intergenic 55.0%
GGCACCATGTCCTGCTCATA+AGG - chr1_4:51746609-51746628 None:intergenic 55.0%
CACGGACATGGTCATGGCCA+TGG + chr1_4:51745247-51745266 Msa0168800:CDS 60.0%
! GGGCCTCCAGGCAATGGATT+TGG + chr1_4:51745274-51745293 Msa0168800:CDS 60.0%
AATGGTCGTGGGCCACAGCT+AGG + chr1_4:51745343-51745362 Msa0168800:CDS 60.0%
ATGGTCGTGGGCCACAGCTA+GGG + chr1_4:51745344-51745363 Msa0168800:CDS 60.0%
CCTGGGATGTTCATGGCAGC+AGG - chr1_4:51745397-51745416 None:intergenic 60.0%
CCTGCTGCCATGAACATCCC+AGG + chr1_4:51745394-51745413 Msa0168800:CDS 60.0%
!! AAGGGTGGTGGGGGAGATTG+GGG + chr1_4:51745598-51745617 Msa0168800:CDS 60.0%
!! TGGGAAAGGTGCTCCTGCCA+AGG + chr1_4:51745897-51745916 Msa0168800:CDS 60.0%
!! GGGAAAGGTGCTCCTGCCAA+GGG + chr1_4:51745898-51745917 Msa0168800:CDS 60.0%
TGGGGGCCATATGGGTCCAA+TGG + chr1_4:51745957-51745976 Msa0168800:CDS 60.0%
GGGGGCCATATGGGTCCAAT+GGG + chr1_4:51745958-51745977 Msa0168800:CDS 60.0%
CTGCCGGCATGCTCCTCATT+TGG - chr1_4:51746056-51746075 None:intergenic 60.0%
!! GGGTGTGATGGCATTGGTGG+CGG - chr1_4:51746348-51746367 None:intergenic 60.0%
TGCCTGGAGGCCCTTGACCA+TGG - chr1_4:51745267-51745286 None:intergenic 65.0%
GGTCAAGGGCCTCCAGGCAA+TGG + chr1_4:51745268-51745287 Msa0168800:CDS 65.0%
CCATCACCAGGCCCTAGCTG+TGG - chr1_4:51745358-51745377 None:intergenic 65.0%
CCACAGCTAGGGCCTGGTGA+TGG + chr1_4:51745355-51745374 Msa0168800:CDS 65.0%
! AGGAGCATGCCGGCAGTGCA+AGG + chr1_4:51746060-51746079 Msa0168800:CDS 65.0%
GGAGCATGCCGGCAGTGCAA+GGG + chr1_4:51746061-51746080 Msa0168800:CDS 65.0%
GCCGGCAGTGCAAGGGTTAC+CGG + chr1_4:51746068-51746087 Msa0168800:CDS 65.0%
GCCGGTAACCCTTGCACTGC+CGG - chr1_4:51746072-51746091 None:intergenic 65.0%
GCAGTGCAAGGGTTACCGGC+CGG + chr1_4:51746072-51746091 Msa0168800:CDS 65.0%
CAGTGCAAGGGTTACCGGCC+GGG + chr1_4:51746073-51746092 Msa0168800:CDS 65.0%
AGTGCAAGGGTTACCGGCCG+GGG + chr1_4:51746074-51746093 Msa0168800:CDS 65.0%
ATTCATCGCCGCAGCTGCCC+CGG - chr1_4:51746094-51746113 None:intergenic 65.0%
GCAGCTGCGGCGATGAATGG+TGG + chr1_4:51746096-51746115 Msa0168800:CDS 65.0%
GGCCATGGTCAAGGGCCTCC+AGG + chr1_4:51745262-51745281 Msa0168800:CDS 70.0%
GGGGCAGCTGCGGCGATGAA+TGG + chr1_4:51746093-51746112 Msa0168800:CDS 70.0%
CGTGGGCCACAGCTAGGGCC+TGG + chr1_4:51745349-51745368 Msa0168800:CDS 75.0%
GTTACCGGCCGGGGCAGCTG+CGG + chr1_4:51746083-51746102 Msa0168800:CDS 75.0%
! ATCGCCGCAGCTGCCCCGGC+CGG - chr1_4:51746090-51746109 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 51743975 51746744 51743975 ID=Msa0168800;Name=Msa0168800
chr1_4 mRNA 51743975 51746744 51743975 ID=Msa0168800-mRNA-1;Parent=Msa0168800;Name=Msa0168800-mRNA-1;_AED=0.01;_eAED=0.01;_QI=262|1|1|1|1|1|3|313|571
chr1_4 exon 51743975 51744266 51743975 ID=Msa0168800-mRNA-1:exon:15251;Parent=Msa0168800-mRNA-1
chr1_4 exon 51744396 51744471 51744396 ID=Msa0168800-mRNA-1:exon:15252;Parent=Msa0168800-mRNA-1
chr1_4 exon 51744822 51746744 51744822 ID=Msa0168800-mRNA-1:exon:15253;Parent=Msa0168800-mRNA-1
chr1_4 five_prime_UTR 51743975 51744236 51743975 ID=Msa0168800-mRNA-1:five_prime_utr;Parent=Msa0168800-mRNA-1
chr1_4 CDS 51744237 51744266 51744237 ID=Msa0168800-mRNA-1:cds;Parent=Msa0168800-mRNA-1
chr1_4 CDS 51744396 51744471 51744396 ID=Msa0168800-mRNA-1:cds;Parent=Msa0168800-mRNA-1
chr1_4 CDS 51744822 51746431 51744822 ID=Msa0168800-mRNA-1:cds;Parent=Msa0168800-mRNA-1
chr1_4 three_prime_UTR 51746432 51746744 51746432 ID=Msa0168800-mRNA-1:three_prime_utr;Parent=Msa0168800-mRNA-1
Gene Sequence

>Msa0168800

ATGAGTAAACAAGATATGATGAAAATTCAGAATTGTCTTCTCAAGGTTAATATCCATTGTGAAGGTTGTGAGCAGAAGGTTAAGAAACTGCTTCAGAAAATTGAAGGGGTGTATTCTGTGAATATAGATGCAGAACAAGGGAAGGTGTTGGTGACAGGTGATGTAGATCCAGCAAAGCTCCTCAAAAAGCTGAAAAGTTCAGGGAAACATGCTGAACTATGGGGTGGACAGAAAGCTATGATGATTAATCAAAATCAAAACTTTCAACAACAACAACCTCAGTTTAAGAACATGCAAATGGATAATAACAAAGGGGGTAAGAACAACAAGCCTCAGAATCAAAAGGGTCAAAAAGGTGGTGTTCAAGTAGCACAGTTTCAAAATCCTAAAGGAGGAAAAGATATGAAAGTGCCCAACAAATCTCAGAAACATGTTAATTTTGATTTGTCTGAGGAGGAATTTGATGAGAGTGATGGTGATTTTGATGACTATGACGATGATTTTGATGAGGATGAAGAAGAGGAGTTTGGTCACGGACATGGTCATGGCCATGGTCAAGGGCCTCCAGGCAATGGATTTGGGCAGCATCCTATGCATAATAACAAGATGATGATGGCTATGATGCAGAATGGTCGTGGGCCACAGCTAGGGCCTGGTGATGGTATGATGATGAATGGGCCTGCTGCCATGAACATCCCAGGTAATGGTAAAGGAGGAGGTGGTGGTAATTATGCAAAAGCCAAAAAAGGAGATATTATGGATCTACCTATGCAAATGCAGATGAAGGGTAAAAGTGGAAATTACGGTGAGGTAAAAAATGGTAATGGAGGTGGTAAGAAAGGGGGTGATGATGGAGGTAAGAAGAATAAAGGGGAAAAGCAAAAGGGTGGTGGGGGAGATTGGGGAGATGAAAAAAATAGTAGCAAGAAGAAAAATGGTAAAGGTAAAAACGGTGGTGGTGGTTTTCTAGTTAAGTTTCTAGGGCTTGGAAAAAAGAGCAAAAAGGGAGGAGGTGCAGCCGACAAGAACAAAAACAATGGAGGAGGTGGAAATAGCAACAACAACAGCAAAGGGAAAGAAGGTAAGAAAGGTGAAAAATTGGATAAAGTTGAATTTGATTTCCAAGACTTTGATATCACTCCTCATGGTAAAAGTGGTAAGGGTGGAAATGGAAATGGAAATGGGAAAGGTGCTCCTGCCAAGGGCAATGCCAATAATGGACATGGTAATAATAATGGTAATGGGGGCCATATGGGTCCAATGGGCCAGATGGGTGCAATGAATCAGATGGCAGCAATGAATAAGATGGGTCCAATGGGTCAGATGGGTTCAATGGACCAAATGAGGAGCATGCCGGCAGTGCAAGGGTTACCGGCCGGGGCAGCTGCGGCGATGAATGGTGGCTATTATCAAGGAATGCAGCAGATGCAACCAAATCCTTATCAGCAACAGCAGCAGCAACAACAACAACAGCAATACATGGCTATGATGATGCAGCAGCAACAGCAGCAACAAATGCAGCAGCAACTGCAGCAGCAACAAATGCAGCAAGGAAATATGAACAGCATGTATCCACAACATATGATGTATGGAGGAGGAAGGCCACATCCATCAATGAACTATATGGGACCGCCACCAATGCCATCACACCCTATGGCAGATCCAATCACTCATGTTTTCAGTGATGAGAACACTGAGAGCTGTAGCATCATGTAA
Protein sequence

>Msa0168800

MSKQDMMKIQNCLLKVNIHCEGCEQKVKKLLQKIEGVYSVNIDAEQGKVLVTGDVDPAKLLKKLKSSGKHAELWGGQKAMMINQNQNFQQQQPQFKNMQMDNNKGGKNNKPQNQKGQKGGVQVAQFQNPKGGKDMKVPNKSQKHVNFDLSEEEFDESDGDFDDYDDDFDEDEEEEFGHGHGHGHGQGPPGNGFGQHPMHNNKMMMAMMQNGRGPQLGPGDGMMMNGPAAMNIPGNGKGGGGGNYAKAKKGDIMDLPMQMQMKGKSGNYGEVKNGNGGGKKGGDDGGKKNKGEKQKGGGGDWGDEKNSSKKKNGKGKNGGGGFLVKFLGLGKKSKKGGGAADKNKNNGGGGNSNNNSKGKEGKKGEKLDKVEFDFQDFDITPHGKSGKGGNGNGNGKGAPAKGNANNGHGNNNGNGGHMGPMGQMGAMNQMAAMNKMGPMGQMGSMDQMRSMPAVQGLPAGAAAAMNGGYYQGMQQMQPNPYQQQQQQQQQQQYMAMMMQQQQQQQMQQQLQQQQMQQGNMNSMYPQHMMYGGGRPHPSMNYMGPPPMPSHPMADPITHVFSDENTESCSIM*