AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0445330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0445330 MtrunA17_Chr3g0085831 98.225 169 3 0 1 169 1 169 6.98e-124 345
Msa0445330 MtrunA17_Chr7g0256851 50.476 105 48 3 1 104 1 102 2.29e-29 104
Msa0445330 MtrunA17_Chr5g0437861 32.749 171 73 4 1 169 4 134 9.69e-21 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0445330 AT2G41430.5 40.571 175 86 6 1 169 1 163 2.94e-28 103
Msa0445330 AT2G41430.4 40.571 175 86 6 1 169 1 163 2.94e-28 103
Msa0445330 AT2G41430.2 40.571 175 86 6 1 169 1 163 2.94e-28 103
Msa0445330 AT2G41430.1 40.571 175 86 6 1 169 1 163 2.94e-28 103
Msa0445330 AT4G14270.1 34.659 176 72 7 2 169 3 143 6.17e-24 91.7
Msa0445330 AT4G14270.2 34.659 176 72 7 2 169 3 143 6.17e-24 91.7
Msa0445330 AT2G41430.3 52.500 80 35 2 1 77 1 80 7.99e-24 90.9

Find 52 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAAATCTTGGAATGTTTA+TGG 0.107441 3_3:+19316559 Msa0445330:three_prime_UTR
AGATAATGAGTTTGAGAAAT+TGG 0.215472 3_3:+19315411 Msa0445330:CDS
CGACATGGTTTCATAACTAT+TGG 0.282742 3_3:+19315263 Msa0445330:CDS
GACTTTGGACCCCTTTCCTT+TGG 0.317537 3_3:-19316272 None:intergenic
GTCCTGTTCAGTGTGGTAAT+TGG 0.318483 3_3:+19316684 Msa0445330:three_prime_UTR
AAATACACTCTTGTAGTAAT+AGG 0.336266 3_3:+19313477 Msa0445330:five_prime_UTR
GCTGGCTTCTCAAAGTGCTT+TGG 0.365521 3_3:-19316308 None:intergenic
GTTCTGATGGCATTGGTAAC+TGG 0.375492 3_3:+19315139 Msa0445330:exon
AGTTTGAACGGCTCAAATCT+TGG 0.379568 3_3:+19316548 Msa0445330:three_prime_UTR
TCCTGTTCAGTGTGGTAATT+GGG 0.386288 3_3:+19316685 Msa0445330:three_prime_UTR
AAGTGCTTTGGAGATCCAAT+AGG 0.400879 3_3:-19316296 None:intergenic
ATTCAATGCTTACCATGAAC+AGG 0.405358 3_3:-19315493 None:intergenic
ACCGTGTGATGGTTCCATTT+TGG 0.414641 3_3:+19316818 Msa0445330:three_prime_UTR
CGAGGTTGGTGAATTCTTTG+AGG 0.416409 3_3:-19316356 None:intergenic
GGAGATCCAATAGGGGACTT+TGG 0.419975 3_3:-19316287 None:intergenic
GGTATGAAGAGAGGTGCATT+TGG 0.424438 3_3:-19315181 None:intergenic
TCAACAGGATCATCCATAAC+TGG 0.427432 3_3:-19315460 None:intergenic
AGGGGTCCAAAGTCCCCTAT+TGG 0.433062 3_3:+19316281 Msa0445330:CDS
AAGAATTCACCAACCTCGTT+AGG 0.433646 3_3:+19316361 Msa0445330:CDS
CATGTAGTGTATTTAATGGT+AGG 0.436501 3_3:+19316514 Msa0445330:three_prime_UTR
AGTGCTTTGGAGATCCAATA+GGG 0.437450 3_3:-19316295 None:intergenic
AGCTCAAGAGTGTCCAGTTA+TGG 0.442394 3_3:+19315447 Msa0445330:CDS
TGAGTTGAAACAGAGCAAAA+TGG 0.452380 3_3:-19313400 None:intergenic
GATCTTGTCACCAAAGGAAA+GGG 0.460246 3_3:+19316262 Msa0445330:CDS
ACCCAATTACCACACTGAAC+AGG 0.469822 3_3:-19316686 None:intergenic
TAACTAATGTATATAGAAGA+AGG 0.499956 3_3:+19316459 Msa0445330:three_prime_UTR
CAAAATCTTCCTCCTTATGC+TGG 0.509472 3_3:-19315291 None:intergenic
CTATTGGTTGAGCCAGCATA+AGG 0.515423 3_3:+19315279 Msa0445330:CDS
TTCTTTGAGGTGCACACTTG+AGG 0.519384 3_3:-19316343 None:intergenic
TTGAGGTTAACATATTGTGC+TGG 0.533520 3_3:-19316326 None:intergenic
ACCTGCTGCATTTCGTCAAG+TGG 0.534431 3_3:+19315201 Msa0445330:CDS
TGGACACTCTTGAGCTTCGT+CGG 0.549850 3_3:-19315440 None:intergenic
TCCACTTGACGAAATGCAGC+AGG 0.554768 3_3:-19315202 None:intergenic
GTCTTAACCAGGTGTTAACA+AGG 0.560735 3_3:+19316220 Msa0445330:intron
AGATCTTGTCACCAAAGGAA+AGG 0.561565 3_3:+19316261 Msa0445330:CDS
GTGAGACTTTCAAGTTTGAA+CGG 0.569472 3_3:+19316536 Msa0445330:three_prime_UTR
TGCTGCATTTCGTCAAGTGG+AGG 0.583030 3_3:+19315204 Msa0445330:CDS
ATTCTGCTGACAAGTTCTGA+TGG 0.587248 3_3:+19315126 Msa0445330:exon
AATGGTTAATGTAAGTTACA+TGG 0.588591 3_3:+19316627 Msa0445330:three_prime_UTR
TATCAAGATCTTGTCACCAA+AGG 0.595830 3_3:+19316256 Msa0445330:CDS
TTCACAAGCTCCCACCATTG+AGG 0.595908 3_3:-19315235 None:intergenic
AGCTTGTGAAGTCTTCGACA+TGG 0.599826 3_3:+19315248 Msa0445330:CDS
ATGACAACTTTATTTAAACA+TGG 0.605146 3_3:+19316794 Msa0445330:three_prime_UTR
TTAAACATGGAACCGTGTGA+TGG 0.607715 3_3:+19316807 Msa0445330:three_prime_UTR
CTGACAAGTTCTGATGGCAT+TGG 0.609871 3_3:+19315132 Msa0445330:exon
GCCAAAATGGAACCATCACA+CGG 0.612324 3_3:-19316819 None:intergenic
AGTTATGGATGATCCTGTTG+AGG 0.618736 3_3:+19315462 Msa0445330:CDS
TTGGTTGAGCCAGCATAAGG+AGG 0.633628 3_3:+19315282 Msa0445330:CDS
ATCTTGTCACCAAAGGAAAG+GGG 0.652126 3_3:+19316263 Msa0445330:CDS
AATGCAGCAGGTATGAAGAG+AGG 0.675950 3_3:-19315190 None:intergenic
TAGGTGTGTCCTGTTCAGTG+TGG 0.680803 3_3:+19316677 Msa0445330:three_prime_UTR
GTGCTTTGGAGATCCAATAG+GGG 0.692880 3_3:-19316294 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATAAATAAATTTTTAGATT+TGG + chr3_3:19314742-19314761 Msa0445330:intron 10.0%
!!! AAAAACAATCTTTTTTTCAA+TGG - chr3_3:19313681-19313700 None:intergenic 15.0%
!! CATCATAAAAAAAATATCAA+TGG - chr3_3:19313715-19313734 None:intergenic 15.0%
!!! TGATGTATGTATTTTAAAAT+AGG + chr3_3:19313924-19313943 Msa0445330:intron 15.0%
!!! GATGTATGTATTTTAAAATA+GGG + chr3_3:19313925-19313944 Msa0445330:intron 15.0%
!!! ATATAAAAACAAGAGTTTTA+TGG + chr3_3:19314378-19314397 Msa0445330:intron 15.0%
!!! TATAAAAACAAGAGTTTTAT+GGG + chr3_3:19314379-19314398 Msa0445330:intron 15.0%
!! TATGTTTATGAAGATATTTA+AGG - chr3_3:19314461-19314480 None:intergenic 15.0%
!!! CTAATTTGTTAAGAATTAAT+CGG - chr3_3:19314665-19314684 None:intergenic 15.0%
!!! AATAAATTTTTAGATTTGGT+TGG + chr3_3:19314746-19314765 Msa0445330:intron 15.0%
!!! ATAAATTTTTAGATTTGGTT+GGG + chr3_3:19314747-19314766 Msa0445330:intron 15.0%
!!! TTCATTTTGAAATAAATGAA+TGG + chr3_3:19316609-19316628 Msa0445330:three_prime_UTR 15.0%
!!! ATGTATTGTAATTTTACATT+TGG + chr3_3:19316719-19316738 Msa0445330:three_prime_UTR 15.0%
!!! ATTGATTTTTTTTTTCCACT+AGG + chr3_3:19313869-19313888 Msa0445330:intron 20.0%
!! AGAGTAAAAAGACAAATAAA+TGG - chr3_3:19314326-19314345 None:intergenic 20.0%
!! TACTCTTCACTAAAAATTTA+AGG + chr3_3:19314340-19314359 Msa0445330:intron 20.0%
!! TATCTTCATAAACATATACT+TGG + chr3_3:19314465-19314484 Msa0445330:intron 20.0%
!! AAATAATAGCAAATGATTTG+AGG - chr3_3:19314920-19314939 None:intergenic 20.0%
!!! AAGCATTGAATCTAATTTAT+TGG + chr3_3:19315505-19315524 Msa0445330:intron 20.0%
!! ACATACAAATCATTGAAAAT+CGG + chr3_3:19315615-19315634 Msa0445330:intron 20.0%
!!! AATACTTTTTCCAACTAAAT+AGG + chr3_3:19315694-19315713 Msa0445330:intron 20.0%
!! GTAAAACAAAATTACATTGT+CGG - chr3_3:19315789-19315808 None:intergenic 20.0%
!! TAGTTTAAAAATCATACGAA+GGG + chr3_3:19315865-19315884 Msa0445330:intron 20.0%
!!! ATTACATTTACTCTAACTTT+TGG - chr3_3:19316073-19316092 None:intergenic 20.0%
!!! TTACATATTTTGTGATTAGT+AGG - chr3_3:19316181-19316200 None:intergenic 20.0%
!! TAACTAATGTATATAGAAGA+AGG + chr3_3:19316459-19316478 Msa0445330:three_prime_UTR 20.0%
!!! TTTTCATGTAGTGTATTTAA+TGG + chr3_3:19316510-19316529 Msa0445330:three_prime_UTR 20.0%
!!! AGTCTATGAATTTATGTTTT+AGG + chr3_3:19316658-19316677 Msa0445330:three_prime_UTR 20.0%
!!! TTTTACATTTGGAAAGAAAA+TGG + chr3_3:19316730-19316749 Msa0445330:three_prime_UTR 20.0%
!!! ATGACAACTTTATTTAAACA+TGG + chr3_3:19316794-19316813 Msa0445330:three_prime_UTR 20.0%
! AAATACACTCTTGTAGTAAT+AGG + chr3_3:19313477-19313496 Msa0445330:five_prime_UTR 25.0%
! TTCTTCAAAAGATCAACAAT+GGG - chr3_3:19313589-19313608 None:intergenic 25.0%
! TTTCTTCAAAAGATCAACAA+TGG - chr3_3:19313590-19313609 None:intergenic 25.0%
! AGAAATAGCATCTATCAAAT+GGG - chr3_3:19313641-19313660 None:intergenic 25.0%
! AAGAAATAGCATCTATCAAA+TGG - chr3_3:19313642-19313661 None:intergenic 25.0%
!! ATTTGAGTCAAGTTTTTATG+TGG + chr3_3:19313791-19313810 Msa0445330:intron 25.0%
! GTTGTTAGTATAGTAAATCT+TGG + chr3_3:19313955-19313974 Msa0445330:intron 25.0%
! TTAGTATAGTAAATCTTGGA+TGG + chr3_3:19313959-19313978 Msa0445330:intron 25.0%
!!! AAGATGAAGTGTTTTTTGAT+TGG + chr3_3:19314070-19314089 Msa0445330:intron 25.0%
!! GAATCAAGTGTTATCTTTTA+TGG + chr3_3:19314141-19314160 Msa0445330:intron 25.0%
!! CTATCTCACAATCAAAATTT+TGG - chr3_3:19314282-19314301 None:intergenic 25.0%
!!! TTGTATCTATTGTTGGTAAT+AGG - chr3_3:19314581-19314600 None:intergenic 25.0%
! TCACAATTTGTATCTATTGT+TGG - chr3_3:19314588-19314607 None:intergenic 25.0%
!!! ATTAGTTTTTGCAATTTGTG+AGG + chr3_3:19314680-19314699 Msa0445330:intron 25.0%
!!! TTAGTTTTTGCAATTTGTGA+GGG + chr3_3:19314681-19314700 Msa0445330:intron 25.0%
!! AATAGCGATTTGTTCAAATT+CGG - chr3_3:19314824-19314843 None:intergenic 25.0%
! AGATAATGAGTTTGAGAAAT+TGG + chr3_3:19315411-19315430 Msa0445330:CDS 25.0%
! CTAGTTTAAAAATCATACGA+AGG + chr3_3:19315864-19315883 Msa0445330:intron 25.0%
! AGATCAATTTGATACAATGT+CGG - chr3_3:19315926-19315945 None:intergenic 25.0%
!! GAATCGAACTCAAAATTTTA+GGG - chr3_3:19316159-19316178 None:intergenic 25.0%
! AATGGTTAATGTAAGTTACA+TGG + chr3_3:19316627-19316646 Msa0445330:three_prime_UTR 25.0%
!! GTGTTGATTTAATTCTGCTA+TGG + chr3_3:19314007-19314026 Msa0445330:intron 30.0%
!! AATTTTTAGATTTGGTTGGG+CGG + chr3_3:19314750-19314769 Msa0445330:intron 30.0%
TCCTATGTAGTATAGAATGT+TGG + chr3_3:19314854-19314873 Msa0445330:intron 30.0%
AGCAAATGATTTGAGGAAAT+AGG - chr3_3:19314913-19314932 None:intergenic 30.0%
AATCTTGTGATCTTAACACA+TGG + chr3_3:19314942-19314961 Msa0445330:intron 30.0%
ATCCTTTATGAACACTTTGT+AGG + chr3_3:19315084-19315103 Msa0445330:intron 30.0%
! ATCCTACAAAGTGTTCATAA+AGG - chr3_3:19315089-19315108 None:intergenic 30.0%
!!! ACACTTTTTTGGATATCTCA+AGG - chr3_3:19315721-19315740 None:intergenic 30.0%
! GATATCCAAAAAAGTGTATG+TGG + chr3_3:19315724-19315743 Msa0445330:intron 30.0%
!!! TATCACCACATACACTTTTT+TGG - chr3_3:19315732-19315751 None:intergenic 30.0%
TGATACAACGTTATATGTCT+TGG + chr3_3:19315747-19315766 Msa0445330:intron 30.0%
! TTGATCTCATACTTTTCTAC+TGG + chr3_3:19315939-19315958 Msa0445330:intron 30.0%
ATATGAATTGCCTCTACTTT+AGG - chr3_3:19315986-19316005 None:intergenic 30.0%
! GGAATCGAACTCAAAATTTT+AGG - chr3_3:19316160-19316179 None:intergenic 30.0%
! AATTGATTTCTGTCTTAACC+AGG + chr3_3:19316209-19316228 Msa0445330:intron 30.0%
! TGTCTTTGAATTTTTCTTGC+TGG + chr3_3:19316487-19316506 Msa0445330:three_prime_UTR 30.0%
CATGTAGTGTATTTAATGGT+AGG + chr3_3:19316514-19316533 Msa0445330:three_prime_UTR 30.0%
CTCAAATCTTGGAATGTTTA+TGG + chr3_3:19316559-19316578 Msa0445330:three_prime_UTR 30.0%
! TGAGTTGAAACAGAGCAAAA+TGG - chr3_3:19313403-19313422 None:intergenic 35.0%
! GAGTCAAGTTTTTATGTGGA+TGG + chr3_3:19313795-19313814 Msa0445330:intron 35.0%
TCCACTAGGAATTGATATGA+TGG + chr3_3:19313883-19313902 Msa0445330:intron 35.0%
CCACTAGGAATTGATATGAT+GGG + chr3_3:19313884-19313903 Msa0445330:intron 35.0%
TCGAAATATCTGCTAGATCT+GGG - chr3_3:19314611-19314630 None:intergenic 35.0%
ATCGAAATATCTGCTAGATC+TGG - chr3_3:19314612-19314631 None:intergenic 35.0%
CGGTCATAGATTTATGGAAT+CGG + chr3_3:19314770-19314789 Msa0445330:intron 35.0%
GCCAACATTCTATACTACAT+AGG - chr3_3:19314858-19314877 None:intergenic 35.0%
TACTCATCAGTGACACTATT+TGG - chr3_3:19314880-19314899 None:intergenic 35.0%
!!! CTGCTATTTTTCAAGATCCA+CGG + chr3_3:19315021-19315040 Msa0445330:intron 35.0%
CGACATGGTTTCATAACTAT+TGG + chr3_3:19315263-19315282 Msa0445330:CDS 35.0%
! GAGGAAGATTTTGTAGAAAG+TGG + chr3_3:19315301-19315320 Msa0445330:CDS 35.0%
! GAAGATTTTGTAGAAAGTGG+CGG + chr3_3:19315304-19315323 Msa0445330:CDS 35.0%
! CTGTCAGAAACTTTTGATCT+TGG + chr3_3:19315364-19315383 Msa0445330:CDS 35.0%
ATTCAATGCTTACCATGAAC+AGG - chr3_3:19315496-19315515 None:intergenic 35.0%
ATCTCAAGGACCTATTTAGT+TGG - chr3_3:19315707-19315726 None:intergenic 35.0%
TGTCACTCAATTGCAATCAT+TGG + chr3_3:19315811-19315830 Msa0445330:intron 35.0%
TATCAAGATCTTGTCACCAA+AGG + chr3_3:19316256-19316275 Msa0445330:CDS 35.0%
TTGAGGTTAACATATTGTGC+TGG - chr3_3:19316329-19316348 None:intergenic 35.0%
!! GTGAGACTTTCAAGTTTGAA+CGG + chr3_3:19316536-19316555 Msa0445330:three_prime_UTR 35.0%
CCCATCATATCAATTCCTAG+TGG - chr3_3:19313887-19313906 None:intergenic 40.0%
AAAACAACAGAGTGACAGCA+AGG - chr3_3:19314434-19314453 None:intergenic 40.0%
GCTATAGCATCATCATTAGC+TGG - chr3_3:19314636-19314655 None:intergenic 40.0%
! ATTCTGCTGACAAGTTCTGA+TGG + chr3_3:19315126-19315145 Msa0445330:exon 40.0%
! TGGAGGATTTTTCACCTCAA+TGG + chr3_3:19315221-19315240 Msa0445330:CDS 40.0%
! AGGATTTTTCACCTCAATGG+TGG + chr3_3:19315224-19315243 Msa0445330:CDS 40.0%
! GGATTTTTCACCTCAATGGT+GGG + chr3_3:19315225-19315244 Msa0445330:CDS 40.0%
CAAAATCTTCCTCCTTATGC+TGG - chr3_3:19315294-19315313 None:intergenic 40.0%
TCAACAGGATCATCCATAAC+TGG - chr3_3:19315463-19315482 None:intergenic 40.0%
AGTTATGGATGATCCTGTTG+AGG + chr3_3:19315462-19315481 Msa0445330:CDS 40.0%
!!! ACAGGCTTTTTTTCCTCAAC+AGG - chr3_3:19315478-19315497 None:intergenic 40.0%
GAGGAAAAAAAGCCTGTTCA+TGG + chr3_3:19315481-19315500 Msa0445330:CDS 40.0%
ATCATACGAAGGGTTATCTG+TGG + chr3_3:19315875-19315894 Msa0445330:intron 40.0%
TAAGCCTCAACCTAAAGTAG+AGG + chr3_3:19315973-19315992 Msa0445330:intron 40.0%
!! ATTGCCTCTACTTTAGGTTG+AGG - chr3_3:19315980-19315999 None:intergenic 40.0%
GTCTTAACCAGGTGTTAACA+AGG + chr3_3:19316220-19316239 Msa0445330:intron 40.0%
AGATCTTGTCACCAAAGGAA+AGG + chr3_3:19316261-19316280 Msa0445330:CDS 40.0%
GATCTTGTCACCAAAGGAAA+GGG + chr3_3:19316262-19316281 Msa0445330:CDS 40.0%
ATCTTGTCACCAAAGGAAAG+GGG + chr3_3:19316263-19316282 Msa0445330:CDS 40.0%
!! AGTGCTTTGGAGATCCAATA+GGG - chr3_3:19316298-19316317 None:intergenic 40.0%
!! AAGTGCTTTGGAGATCCAAT+AGG - chr3_3:19316299-19316318 None:intergenic 40.0%
AAGAATTCACCAACCTCGTT+AGG + chr3_3:19316361-19316380 Msa0445330:CDS 40.0%
! CAAGTTTTTCCTAACGAGGT+TGG - chr3_3:19316373-19316392 None:intergenic 40.0%
!! GCTTCAAGTTTTTCCTAACG+AGG - chr3_3:19316377-19316396 None:intergenic 40.0%
AGTTTGAACGGCTCAAATCT+TGG + chr3_3:19316548-19316567 Msa0445330:three_prime_UTR 40.0%
TCCTGTTCAGTGTGGTAATT+GGG + chr3_3:19316685-19316704 Msa0445330:three_prime_UTR 40.0%
TTAAACATGGAACCGTGTGA+TGG + chr3_3:19316807-19316826 Msa0445330:three_prime_UTR 40.0%
GTTGGGCGGTCATAGATTTA+TGG + chr3_3:19314764-19314783 Msa0445330:intron 45.0%
TAGAACAGTGTTGTCAACCG+TGG - chr3_3:19315041-19315060 None:intergenic 45.0%
! CTGACAAGTTCTGATGGCAT+TGG + chr3_3:19315132-19315151 Msa0445330:exon 45.0%
!! GTTCTGATGGCATTGGTAAC+TGG + chr3_3:19315139-19315158 Msa0445330:exon 45.0%
! GGTATGAAGAGAGGTGCATT+TGG - chr3_3:19315184-19315203 None:intergenic 45.0%
AATGCAGCAGGTATGAAGAG+AGG - chr3_3:19315193-19315212 None:intergenic 45.0%
AGCTTGTGAAGTCTTCGACA+TGG + chr3_3:19315248-19315267 Msa0445330:CDS 45.0%
! CTATTGGTTGAGCCAGCATA+AGG + chr3_3:19315279-19315298 Msa0445330:CDS 45.0%
!! AGCTCAAGAGTGTCCAGTTA+TGG + chr3_3:19315447-19315466 Msa0445330:CDS 45.0%
TACGAAGGGTTATCTGTGGT+TGG + chr3_3:19315879-19315898 Msa0445330:intron 45.0%
AAGGGTTATCTGTGGTTGGT+CGG + chr3_3:19315883-19315902 Msa0445330:intron 45.0%
TGTAATGCGTCAAACCACCA+AGG + chr3_3:19316087-19316106 Msa0445330:intron 45.0%
TAACTACTTGCGCTACACCT+TGG - chr3_3:19316107-19316126 None:intergenic 45.0%
!! GTGCTTTGGAGATCCAATAG+GGG - chr3_3:19316297-19316316 None:intergenic 45.0%
!! TTCTTTGAGGTGCACACTTG+AGG - chr3_3:19316346-19316365 None:intergenic 45.0%
!! CGAGGTTGGTGAATTCTTTG+AGG - chr3_3:19316359-19316378 None:intergenic 45.0%
GTCCTGTTCAGTGTGGTAAT+TGG + chr3_3:19316684-19316703 Msa0445330:three_prime_UTR 45.0%
ACCCAATTACCACACTGAAC+AGG - chr3_3:19316689-19316708 None:intergenic 45.0%
ACCTGCTGCATTTCGTCAAG+TGG + chr3_3:19315201-19315220 Msa0445330:CDS 50.0%
TCCACTTGACGAAATGCAGC+AGG - chr3_3:19315205-19315224 None:intergenic 50.0%
TGCTGCATTTCGTCAAGTGG+AGG + chr3_3:19315204-19315223 Msa0445330:CDS 50.0%
TTCACAAGCTCCCACCATTG+AGG - chr3_3:19315238-19315257 None:intergenic 50.0%
TTGGTTGAGCCAGCATAAGG+AGG + chr3_3:19315282-19315301 Msa0445330:CDS 50.0%
TGGACACTCTTGAGCTTCGT+CGG - chr3_3:19315443-19315462 None:intergenic 50.0%
TTAGTGCGCACTGTGTGTCA+GGG + chr3_3:19316124-19316143 Msa0445330:intron 50.0%
GCAGCATCCTTGTTAACACC+TGG - chr3_3:19316230-19316249 None:intergenic 50.0%
GACTTTGGACCCCTTTCCTT+TGG - chr3_3:19316275-19316294 None:intergenic 50.0%
GGAGATCCAATAGGGGACTT+TGG - chr3_3:19316290-19316309 None:intergenic 50.0%
! GCTGGCTTCTCAAAGTGCTT+TGG - chr3_3:19316311-19316330 None:intergenic 50.0%
TAGGTGTGTCCTGTTCAGTG+TGG + chr3_3:19316677-19316696 Msa0445330:three_prime_UTR 50.0%
CTACTTGCGCTACACCTTGG+TGG - chr3_3:19316104-19316123 None:intergenic 55.0%
GTTAGTGCGCACTGTGTGTC+AGG + chr3_3:19316123-19316142 Msa0445330:intron 55.0%
AGGGGTCCAAAGTCCCCTAT+TGG + chr3_3:19316281-19316300 Msa0445330:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 19313387 19316838 19313387 ID=Msa0445330;Name=Msa0445330
chr3_3 mRNA 19313387 19316838 19313387 ID=Msa0445330-mRNA-1;Parent=Msa0445330;Name=Msa0445330-mRNA-1;_AED=0.00;_eAED=0.00;_QI=151|1|1|1|0.5|0.33|3|456|169
chr3_3 exon 19313387 19313498 19313387 ID=Msa0445330-mRNA-1:exon:4106;Parent=Msa0445330-mRNA-1
chr3_3 exon 19315106 19315502 19315106 ID=Msa0445330-mRNA-1:exon:4107;Parent=Msa0445330-mRNA-1
chr3_3 exon 19316231 19316838 19316231 ID=Msa0445330-mRNA-1:exon:4108;Parent=Msa0445330-mRNA-1
chr3_3 five_prime_UTR 19313387 19313498 19313387 ID=Msa0445330-mRNA-1:five_prime_utr;Parent=Msa0445330-mRNA-1
chr3_3 five_prime_UTR 19315106 19315144 19315106 ID=Msa0445330-mRNA-1:five_prime_utr;Parent=Msa0445330-mRNA-1
chr3_3 CDS 19315145 19315502 19315145 ID=Msa0445330-mRNA-1:cds;Parent=Msa0445330-mRNA-1
chr3_3 CDS 19316231 19316382 19316231 ID=Msa0445330-mRNA-1:cds;Parent=Msa0445330-mRNA-1
chr3_3 three_prime_UTR 19316383 19316838 19316383 ID=Msa0445330-mRNA-1:three_prime_utr;Parent=Msa0445330-mRNA-1
Gene Sequence

>Msa0445330

ATGGCATTGGTAACTGGAAGAAGTTCAGCTCTGAATCCAAATGCACCTCTCTTCATACCTGCTGCATTTCGTCAAGTGGAGGATTTTTCACCTCAATGGTGGGAGCTTGTGAAGTCTTCGACATGGTTTCATAACTATTGGTTGAGCCAGCATAAGGAGGAAGATTTTGTAGAAAGTGGCGGTAATGATGCTACAAATGATGATGACATAGAACATATGCTGTCAGAAACTTTTGATCTTGGCATTGAAGAAGATTTCAACTTTCTAGATAATGAGTTTGAGAAATTGGTGATGTCCGACGAAGCTCAAGAGTGTCCAGTTATGGATGATCCTGTTGAGGAAAAAAAGCCTGTTCATGGTGTTAACAAGGATGCTGCAGCTCTTATCAAGATCTTGTCACCAAAGGAAAGGGGTCCAAAGTCCCCTATTGGATCTCCAAAGCACTTTGAGAAGCCAGCACAATATGTTAACCTCAAGTGTGCACCTCAAAGAATTCACCAACCTCGTTAG
Protein sequence

>Msa0445330

MALVTGRSSALNPNAPLFIPAAFRQVEDFSPQWWELVKSSTWFHNYWLSQHKEEDFVESGGNDATNDDDIEHMLSETFDLGIEEDFNFLDNEFEKLVMSDEAQECPVMDDPVEEKKPVHGVNKDAAALIKILSPKERGPKSPIGSPKHFEKPAQYVNLKCAPQRIHQPR*