AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1377570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1377570 MtrunA17_Chr7g0260841 98.012 503 10 0 1 503 1 503 0.0 1027
Msa1377570 MtrunA17_Chr7g0275821 54.348 184 65 3 335 502 232 412 9.09e-51 179
Msa1377570 MtrunA17_Chr7g0275821 30.387 181 94 7 5 158 6 181 1.07e-14 76.3
Msa1377570 MtrunA17_Chr8g0346161 28.306 484 242 15 43 481 19 442 1.75e-42 157
Msa1377570 MtrunA17_Chr5g0411341 42.584 209 81 3 328 501 466 670 9.84e-40 152
Msa1377570 MtrunA17_Chr5g0411341 25.967 181 126 4 49 223 44 222 2.37e-11 66.2
Msa1377570 MtrunA17_Chr8g0346151 27.571 457 220 10 63 475 38 427 4.52e-38 145
Msa1377570 MtrunA17_Chr8g0366751 43.575 179 66 3 337 480 444 622 3.72e-37 145
Msa1377570 MtrunA17_Chr8g0366751 28.804 184 123 6 49 226 53 234 1.09e-12 70.5
Msa1377570 MtrunA17_Chr4g0049331 56.250 64 28 0 353 416 133 196 7.66e-17 81.3
Msa1377570 MtrunA17_Chr5g0400661 46.591 88 42 2 333 416 123 209 3.00e-16 80.1
Msa1377570 MtrunA17_Chr1g0185621 42.857 98 51 2 326 419 443 539 2.19e-14 75.9
Msa1377570 MtrunA17_Chr1g0211081 43.182 88 49 1 324 410 285 372 3.41e-14 75.1
Msa1377570 MtrunA17_Chr4g0054561 46.341 82 38 2 336 417 140 215 4.32e-14 73.6
Msa1377570 MtrunA17_Chr4g0033391 31.765 170 77 4 245 408 64 200 6.12e-14 72.8
Msa1377570 MtrunA17_Chr2g0280161 54.386 57 26 0 355 411 177 233 9.35e-14 72.8
Msa1377570 MtrunA17_Chr2g0298751 42.308 104 46 4 356 445 53 156 1.40e-13 70.5
Msa1377570 MtrunA17_Chr4g0054571 53.704 54 25 0 355 408 150 203 3.98e-13 70.5
Msa1377570 MtrunA17_Chr2g0307131 38.182 110 55 3 299 408 136 232 4.38e-13 70.5
Msa1377570 MtrunA17_Chr5g0411331 27.957 186 116 6 49 223 27 205 7.41e-13 68.6
Msa1377570 MtrunA17_Chr4g0033281 37.113 97 51 1 312 408 120 206 7.67e-13 69.7
Msa1377570 MtrunA17_Chr4g0054551 38.462 104 56 3 341 441 138 236 1.42e-12 68.9
Msa1377570 MtrunA17_Chr3g0131691 53.448 58 27 0 353 410 296 353 2.34e-12 68.9
Msa1377570 MtrunA17_Chr5g0400671 32.231 121 57 2 342 441 145 261 2.57e-12 68.2
Msa1377570 MtrunA17_Chr7g0225901 40.625 96 51 3 313 408 52 141 2.64e-12 67.0
Msa1377570 MtrunA17_Chr8g0365711 22.129 357 177 12 188 496 111 414 2.76e-12 68.6
Msa1377570 MtrunA17_Chr4g0054581 45.588 68 31 1 341 408 190 251 3.53e-12 68.2
Msa1377570 MtrunA17_Chr5g0400621 43.836 73 39 1 338 410 142 212 3.80e-12 67.4
Msa1377570 MtrunA17_Chr7g0252041 51.724 58 28 0 353 410 284 341 4.48e-12 68.2
Msa1377570 MtrunA17_Chr2g0333621 35.652 115 66 4 308 418 90 200 6.58e-12 66.6
Msa1377570 MtrunA17_Chr8g0386921 50.820 61 30 0 351 411 402 462 6.77e-12 67.8
Msa1377570 MtrunA17_Chr4g0033291 30.488 164 95 3 245 408 64 208 7.15e-12 66.6
Msa1377570 MtrunA17_Chr5g0400641 50.000 52 26 0 359 410 2 53 8.73e-12 63.5
Msa1377570 MtrunA17_Chr6g0468461 51.852 54 26 0 355 408 173 226 1.49e-11 65.9
Msa1377570 MtrunA17_Chr4g0054621 33.884 121 67 2 297 408 109 225 2.08e-11 65.5
Msa1377570 MtrunA17_Chr2g0307141 51.852 54 26 0 355 408 183 236 2.45e-11 65.5
Msa1377570 MtrunA17_Chr2g0333641 32.031 128 82 3 308 433 89 213 3.14e-11 64.7
Msa1377570 MtrunA17_Chr4g0028071 43.939 66 34 1 345 410 142 204 6.73e-11 63.9
Msa1377570 MtrunA17_Chr2g0320291 28.000 150 86 4 359 486 137 286 7.11e-11 63.5
Msa1377570 MtrunA17_Chr6g0468491 50.000 54 27 0 355 408 137 190 8.75e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1377570 AT4G16430.1 47.431 506 220 11 3 502 2 467 5.53e-159 460
Msa1377570 AT2G46510.1 35.000 560 272 14 9 500 9 544 2.34e-93 295
Msa1377570 AT1G01260.1 52.096 167 72 3 338 502 416 576 1.21e-47 174
Msa1377570 AT1G01260.1 36.431 269 138 8 5 245 5 268 1.06e-40 155
Msa1377570 AT1G01260.2 52.096 167 72 3 338 502 416 576 1.21e-47 174
Msa1377570 AT1G01260.2 36.431 269 138 8 5 245 5 268 1.06e-40 155
Msa1377570 AT1G01260.3 52.096 167 72 3 338 502 416 576 1.21e-47 174
Msa1377570 AT1G01260.3 36.431 269 138 8 5 245 5 268 1.06e-40 155
Msa1377570 AT4G17880.1 27.843 510 286 13 49 481 62 566 5.06e-45 167
Msa1377570 AT1G32640.1 42.105 190 81 2 336 500 433 618 1.05e-36 144
Msa1377570 AT5G46760.1 40.816 196 88 1 336 503 396 591 3.99e-36 142
Msa1377570 AT5G46830.1 28.364 483 290 15 41 481 23 491 4.71e-35 137
Msa1377570 AT4G00870.1 37.059 170 73 5 333 468 220 389 1.76e-20 94.4
Msa1377570 AT4G00480.3 24.742 388 245 13 50 409 23 391 3.83e-20 94.0
Msa1377570 AT4G00480.1 24.742 388 245 13 50 409 23 391 4.33e-20 93.6
Msa1377570 AT4G00480.2 24.742 388 245 13 50 409 23 391 4.85e-20 94.0
Msa1377570 AT4G29930.6 27.419 186 111 3 327 493 31 211 2.24e-15 75.9
Msa1377570 AT2G16910.1 27.881 269 147 9 195 422 121 383 2.88e-15 79.0
Msa1377570 AT4G29930.2 26.776 183 113 4 327 493 31 208 6.71e-15 74.3
Msa1377570 AT5G57150.5 46.512 86 33 1 325 410 37 109 8.14e-15 74.3
Msa1377570 AT5G57150.3 46.512 86 33 1 325 410 38 110 9.07e-15 73.9
Msa1377570 AT5G57150.2 46.512 86 33 1 325 410 38 110 9.70e-15 73.9
Msa1377570 AT5G57150.1 46.512 86 33 1 325 410 37 109 1.44e-14 73.9
Msa1377570 AT5G57150.4 46.512 86 33 1 325 410 38 110 1.75e-14 73.9
Msa1377570 AT4G37850.2 49.333 75 38 0 337 411 134 208 2.64e-14 74.3
Msa1377570 AT4G37850.1 49.333 75 38 0 337 411 134 208 2.64e-14 74.3
Msa1377570 AT1G63650.3 47.761 67 35 0 351 417 401 467 5.96e-14 74.7
Msa1377570 AT1G63650.3 26.961 204 126 4 42 229 9 205 3.00e-13 72.8
Msa1377570 AT1G63650.1 47.761 67 35 0 351 417 401 467 5.96e-14 74.7
Msa1377570 AT1G63650.1 26.961 204 126 4 42 229 9 205 3.00e-13 72.8
Msa1377570 AT1G63650.2 47.761 67 35 0 351 417 401 467 5.96e-14 74.7
Msa1377570 AT1G63650.2 26.961 204 126 4 42 229 9 205 3.00e-13 72.8
Msa1377570 AT5G41315.3 25.446 224 125 6 42 238 15 223 1.18e-13 73.9
Msa1377570 AT5G41315.3 39.130 69 42 0 349 417 440 508 1.56e-11 67.4
Msa1377570 AT5G41315.2 25.446 224 125 6 42 238 18 226 1.28e-13 73.9
Msa1377570 AT5G41315.2 39.130 69 42 0 349 417 443 511 1.58e-11 67.4
Msa1377570 AT5G41315.1 25.446 224 125 6 42 238 10 218 1.32e-13 73.9
Msa1377570 AT5G41315.1 39.130 69 42 0 349 417 435 503 1.55e-11 67.4
Msa1377570 AT4G09820.1 27.108 166 106 2 335 496 354 508 1.95e-13 73.2
Msa1377570 AT4G29930.3 38.835 103 56 2 327 427 31 128 2.08e-13 70.9
Msa1377570 AT4G29930.7 38.835 103 56 2 327 427 31 128 2.08e-13 70.9
Msa1377570 AT4G29930.5 26.901 171 104 4 327 481 31 196 2.39e-13 70.1
Msa1377570 AT4G29930.1 38.835 103 56 2 327 427 31 128 2.57e-13 70.5
Msa1377570 AT4G29930.4 38.835 103 56 2 327 427 31 128 2.63e-13 68.9
Msa1377570 AT2G22750.2 51.562 64 31 0 341 404 112 175 4.11e-13 70.5
Msa1377570 AT2G22750.4 51.562 64 31 0 341 404 140 203 4.14e-13 70.9
Msa1377570 AT2G22750.1 51.562 64 31 0 341 404 112 175 4.14e-13 70.5
Msa1377570 AT2G22750.3 51.562 64 31 0 341 404 140 203 4.17e-13 70.9
Msa1377570 AT2G22750.5 51.562 64 31 0 341 404 136 199 4.26e-13 70.9
Msa1377570 AT1G12860.2 55.172 58 26 0 353 410 265 322 5.75e-13 71.2
Msa1377570 AT1G12860.1 55.172 58 26 0 353 410 265 322 6.64e-13 71.2
Msa1377570 AT2G22770.1 44.872 78 40 1 349 423 123 200 1.34e-12 69.3
Msa1377570 AT3G26744.1 53.448 58 27 0 353 410 305 362 2.35e-12 69.7
Msa1377570 AT3G26744.4 53.448 58 27 0 353 410 305 362 2.35e-12 69.7
Msa1377570 AT3G26744.2 53.448 58 27 0 353 410 305 362 2.35e-12 69.7
Msa1377570 AT3G26744.6 53.448 58 27 0 353 410 305 362 2.41e-12 69.3
Msa1377570 AT5G10570.1 36.220 127 70 3 299 416 89 213 6.68e-11 63.9

Find 121 sgRNAs with CRISPR-Local

Find 324 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGATATCCACCCGGTTTC+TGG 0.132043 tig0019933:-122742 Msa1377570:CDS
ATGGACAAGGCTTCTCTTCT+TGG 0.203486 tig0019933:-122919 Msa1377570:CDS
ATATGAATCGAGGTCGTTTC+TGG 0.204427 tig0019933:-123535 Msa1377570:CDS
CCAGAATCATCTTCCACTTT+TGG 0.206725 tig0019933:+123288 None:intergenic
TGAGAATATTTATAAGAAAT+TGG 0.206945 tig0019933:-123697 Msa1377570:CDS
TGTTCCTAACATTTCTAAAA+TGG 0.208602 tig0019933:-122938 Msa1377570:CDS
TGATGTTAGGAGGAAGGTTT+TGG 0.250788 tig0019933:-123757 Msa1377570:CDS
CCATAAGCTTGATCTTCTTC+TGG 0.258919 tig0019933:+122869 None:intergenic
AGAGTTTCTTCTATTGATTT+AGG 0.261536 tig0019933:-123117 Msa1377570:CDS
TGGGGAAGATTCATGGTTGC+TGG 0.279240 tig0019933:-123737 Msa1377570:CDS
TCCCTTTCCTGCTCATTCCT+TGG 0.291476 tig0019933:+123786 None:intergenic
ACCTCTGTCTATTATGTGTT+TGG 0.301401 tig0019933:-123642 Msa1377570:CDS
CCAAAAGTGGAAGATGATTC+TGG 0.302301 tig0019933:-123288 Msa1377570:CDS
TCTTCTGGAGATCAGTGATA+TGG 0.305181 tig0019933:+122884 None:intergenic
CCAGAAGAAGATCAAGCTTA+TGG 0.312383 tig0019933:-122869 Msa1377570:CDS
GATATGGTCAGGGCAACTTT+TGG 0.330665 tig0019933:-123411 Msa1377570:CDS
GGGGAAGATTCATGGTTGCT+GGG 0.377703 tig0019933:-123736 Msa1377570:CDS
TAAGAAATTGGACAGTGTTT+CGG 0.382697 tig0019933:-123685 Msa1377570:CDS
CAGTTGGGTCAAATGATTCC+TGG 0.383826 tig0019933:-123159 Msa1377570:CDS
CAGGTTGCCGGTTTGAAATC+CGG 0.384873 tig0019933:-123861 Msa1377570:CDS
AAAGGGAGAAAGATGATGTT+AGG 0.390427 tig0019933:-123770 Msa1377570:CDS
TGGATAGTCACGTCAAGATT+CGG 0.396746 tig0019933:+123939 None:intergenic
ATGTATGAATTACTTTATCC+TGG 0.397717 tig0019933:+122659 None:intergenic
GTTAGGAACAACAGCTCTTA+AGG 0.401789 tig0019933:+122951 None:intergenic
GAGGTCGTTTCTGGCTAAAT+CGG 0.407099 tig0019933:-123526 Msa1377570:CDS
GCACACTTAAAAGAATTTCC+AGG 0.410186 tig0019933:+123600 None:intergenic
ACTTTAACAATTCCAGAAAC+CGG 0.412032 tig0019933:+122730 None:intergenic
CTTGGCGCAAACCATGCTTA+TGG 0.414623 tig0019933:-123234 Msa1377570:CDS
AAGATTCATGGTTGCTGGGG+TGG 0.418070 tig0019933:-123732 Msa1377570:CDS
TTTGGGCACGAGTTAAGCTT+GGG 0.421749 tig0019933:-123348 Msa1377570:CDS
CTCTTAAGGCGTAAAATCTC+TGG 0.435497 tig0019933:+122965 None:intergenic
GGTTCCTCTCGTCCATTGGC+AGG 0.435606 tig0019933:+123033 None:intergenic
CTTATGGTAATTCTTCCAAC+GGG 0.438633 tig0019933:-123218 Msa1377570:CDS
CAGTGGTTCCTCTCGTCCAT+TGG 0.439512 tig0019933:+123029 None:intergenic
AGGTTGTGTCACATTGTTAA+CGG 0.439902 tig0019933:-123912 Msa1377570:CDS
GAAAGATGATGTTAGGAGGA+AGG 0.440512 tig0019933:-123763 Msa1377570:CDS
TATCTTGCAATGGCAATTGC+TGG 0.440816 tig0019933:+122815 None:intergenic
TTTAATTCTCAGTATGGTCC+TGG 0.444163 tig0019933:-123618 Msa1377570:CDS
GAAGACTCCTCCTCACCTCT+TGG 0.464557 tig0019933:-123090 Msa1377570:CDS
TGAAGAAGACAAGGCTATGT+TGG 0.466385 tig0019933:-124042 Msa1377570:CDS
TATTAGTTTCTCCCCAAAAG+TGG 0.469095 tig0019933:-123301 Msa1377570:CDS
TTCCCCTCCTTGAGTTCTGA+TGG 0.471316 tig0019933:+122633 None:intergenic
TCTATAACCCCGGCTTTAAG+AGG 0.476638 tig0019933:+123477 None:intergenic
ATCTACCTCTGGACAGGCTA+AGG 0.484847 tig0019933:-123385 Msa1377570:CDS
ATACGGTGAGGGACACTGTC+AGG 0.487755 tig0019933:-123823 Msa1377570:CDS
TCGTTTCTGGCTAAATCGGC+TGG 0.495546 tig0019933:-123522 Msa1377570:CDS
GGCCAATGTTTCAACTTCCC+AGG 0.496533 tig0019933:-122677 Msa1377570:CDS
CCAAGTTGGTCTTCTGATGC+TGG 0.500304 tig0019933:-123573 Msa1377570:CDS
CTTCTGGAGATCAGTGATAT+GGG 0.500322 tig0019933:+122885 None:intergenic
GTCCAAGGAATGAGCAGGAA+AGG 0.501601 tig0019933:-123788 Msa1377570:CDS
GGCTATGTTGGAATCTGTGT+TGG 0.505854 tig0019933:-124030 Msa1377570:CDS
AAAGCCGGGGTTATAGAGCT+TGG 0.507196 tig0019933:-123471 Msa1377570:CDS
TGGAGAATCTACCTCTGGAC+AGG 0.507498 tig0019933:-123391 Msa1377570:CDS
TGTTAGAGTTAGCTGCCCCT+TGG 0.517530 tig0019933:-122761 Msa1377570:CDS
TCCAAGGAATGAGCAGGAAA+GGG 0.524680 tig0019933:-123787 Msa1377570:CDS
GACACTGTCAGGATCCCAAA+GGG 0.526579 tig0019933:-123812 Msa1377570:CDS
ATCCTGGGAAGTTGAAACAT+TGG 0.527219 tig0019933:+122675 None:intergenic
CTGCCCCTTGGATATCCACC+CGG 0.528394 tig0019933:-122749 Msa1377570:CDS
TTGACGTGACTATCCACAAA+AGG 0.529097 tig0019933:-123932 Msa1377570:CDS
GGTTATAGAGCTTGGTTCGA+CGG 0.532928 tig0019933:-123463 Msa1377570:CDS
AGAGCTTGGTTCGACGGAGA+TGG 0.536663 tig0019933:-123457 Msa1377570:CDS
CAAAATCGCGTAGTTCCACT+TGG 0.541178 tig0019933:+123887 None:intergenic
AGAGAAAGATGGCTGGTGAG+AGG 0.545343 tig0019933:-124076 None:intergenic
TTAAGTGTGCTAAACCAAGT+TGG 0.545549 tig0019933:-123587 Msa1377570:CDS
GGGAAGATTCATGGTTGCTG+GGG 0.546890 tig0019933:-123735 Msa1377570:CDS
TGTATGAATTACTTTATCCT+GGG 0.549481 tig0019933:+122660 None:intergenic
ATAGCCACCGGATTTCAAAC+CGG 0.550635 tig0019933:+123854 None:intergenic
GCCTGAGAAGTAAAATTCCC+AGG 0.550809 tig0019933:+123141 None:intergenic
GCATCAAATTGTAGCTCAAG+AGG 0.553064 tig0019933:-122698 Msa1377570:CDS
TTGAAGCGGAGAGGCAAAGA+CGG 0.553502 tig0019933:-122999 Msa1377570:CDS
GCTTATGGTAATTCTTCCAA+CGG 0.554315 tig0019933:-123219 Msa1377570:CDS
CCAGCATCAGAAGACCAACT+TGG 0.555302 tig0019933:+123573 None:intergenic
AGTTGGGTCAAATGATTCCT+GGG 0.557673 tig0019933:-123158 Msa1377570:CDS
TGGAAGAATTACCATAAGCA+TGG 0.563019 tig0019933:+123223 None:intergenic
ACATTCTCCATCAGAACTCA+AGG 0.569500 tig0019933:-122640 Msa1377570:CDS
CTTTAACAATTCCAGAAACC+GGG 0.569555 tig0019933:+122731 None:intergenic
GGGAGAAAGATGATGTTAGG+AGG 0.569685 tig0019933:-123767 Msa1377570:CDS
TCCGCTTCAACATGATTCAG+TGG 0.571772 tig0019933:+123012 None:intergenic
TGAAGCGGAGAGGCAAAGAC+GGG 0.572704 tig0019933:-122998 Msa1377570:CDS
GGTGGCTATGTGTTGAAATA+CGG 0.578846 tig0019933:-123840 Msa1377570:CDS
AGAAACCGGGTGGATATCCA+AGG 0.585451 tig0019933:+122744 None:intergenic
TGGGCACGAGTTAAGCTTGG+GGG 0.592030 tig0019933:-123346 Msa1377570:CDS
TGAAAATCAATATCTTGCAA+TGG 0.592942 tig0019933:+122805 None:intergenic
CTCCATCAGAACTCAAGGAG+GGG 0.593410 tig0019933:-122635 Msa1377570:CDS
GTATAGTAGAGAAAGATGGC+TGG 0.593720 tig0019933:-124083 None:intergenic
TAACATTTCTAAAATGGACA+AGG 0.599447 tig0019933:-122932 Msa1377570:CDS
CTCGTCTCCAAGAGGTGAGG+AGG 0.602197 tig0019933:+123083 None:intergenic
AGAGGGAGAAAACCTGCCAA+TGG 0.605353 tig0019933:-123045 Msa1377570:CDS
ACCACTGAATCATGTTGAAG+CGG 0.606334 tig0019933:-123013 Msa1377570:CDS
GTATTTCAACACATAGCCAC+CGG 0.614051 tig0019933:+123842 None:intergenic
GCTATGTTGGAATCTGTGTT+GGG 0.620643 tig0019933:-124029 Msa1377570:CDS
GGACACTGTCAGGATCCCAA+AGG 0.622420 tig0019933:-123813 Msa1377570:CDS
TCTCCATCAGAACTCAAGGA+GGG 0.623115 tig0019933:-122636 Msa1377570:CDS
CACTGTCAGGATCCCAAAGG+GGG 0.625514 tig0019933:-123810 Msa1377570:CDS
GTTTAAATCAATATGAATCG+AGG 0.626645 tig0019933:-123545 Msa1377570:CDS
TTGTAAGAATGAAGAAGACA+AGG 0.629373 tig0019933:-124051 Msa1377570:CDS
GAATCATTTGACCCAACTGA+GGG 0.642194 tig0019933:+123163 None:intergenic
CCGCTCGTCTCCAAGAGGTG+AGG 0.645733 tig0019933:+123080 None:intergenic
CCTCACCTCTTGGAGACGAG+CGG 0.646158 tig0019933:-123080 Msa1377570:CDS
TATGGTAATTCTTCCAACGG+GGG 0.650209 tig0019933:-123216 Msa1377570:CDS
TTCTCCATCAGAACTCAAGG+AGG 0.654759 tig0019933:-122637 Msa1377570:CDS
AAAACCTGCCAATGGACGAG+AGG 0.654921 tig0019933:-123037 Msa1377570:CDS
GAAACCGGGTGGATATCCAA+GGG 0.657964 tig0019933:+122745 None:intergenic
TGAATCATGTTGAAGCGGAG+AGG 0.659736 tig0019933:-123008 Msa1377570:CDS
GGAATCATTTGACCCAACTG+AGG 0.660288 tig0019933:+123162 None:intergenic
GGCTTCCGCTCGTCTCCAAG+AGG 0.660984 tig0019933:+123075 None:intergenic
GTTGCCGGTTTGAAATCCGG+TGG 0.661652 tig0019933:-123858 Msa1377570:CDS
ACATTGTTAACGGTTCCAAG+TGG 0.664341 tig0019933:-123902 Msa1377570:CDS
TTGGGCACGAGTTAAGCTTG+GGG 0.666411 tig0019933:-123347 Msa1377570:CDS
CGAACCAAGCTCTATAACCC+CGG 0.673641 tig0019933:+123467 None:intergenic
GAGATGGTGCCTGAAGAGCA+AGG 0.686748 tig0019933:-123441 Msa1377570:CDS
ACACTGTCAGGATCCCAAAG+GGG 0.690461 tig0019933:-123811 Msa1377570:CDS
TAACAATTCCAGAAACCGGG+TGG 0.692180 tig0019933:+122734 None:intergenic
ACCAAACACATAATAGACAG+AGG 0.698940 tig0019933:+123641 None:intergenic
ATTCGGCGGAACAATAACAT+CGG 0.702866 tig0019933:+123956 None:intergenic
TATGTGTTGAAATACGGTGA+GGG 0.713229 tig0019933:-123834 Msa1377570:CDS
TTATGGTAATTCTTCCAACG+GGG 0.725236 tig0019933:-123217 Msa1377570:CDS
ATAGTCACGTCAAGATTCGG+CGG 0.725406 tig0019933:+123942 None:intergenic
AAACCGGGTGGATATCCAAG+GGG 0.728834 tig0019933:+122746 None:intergenic
CTATGTGTTGAAATACGGTG+AGG 0.742411 tig0019933:-123835 Msa1377570:CDS
GGGTGTAGGAGACAGCAACG+AGG 0.803375 tig0019933:-123196 Msa1377570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAGAATATTTATAAGAAAT+TGG - tig0019933:122950-122969 Msa1377570:CDS 15.0%
!! TGAGAATATTTATAAGAAAT+TGG - tig0019933:122950-122969 Msa1377570:CDS 15.0%
!!! TTTGGTTTTAATTCTCAGTA+TGG - tig0019933:123023-123042 Msa1377570:CDS 25.0%
! GTTTAAATCAATATGAATCG+AGG - tig0019933:123102-123121 Msa1377570:CDS 25.0%
!!! AGAGTTTCTTCTATTGATTT+AGG - tig0019933:123530-123549 Msa1377570:CDS 25.0%
!! TGTTCCTAACATTTCTAAAA+TGG - tig0019933:123709-123728 Msa1377570:CDS 25.0%
!!! TTGTCCATTTTAGAAATGTT+AGG + tig0019933:123716-123735 None:intergenic 25.0%
!!! TAACATTTCTAAAATGGACA+AGG - tig0019933:123715-123734 Msa1377570:CDS 25.0%
! TATGGAAACTGAAAAAAACA+TGG - tig0019933:123796-123815 Msa1377570:CDS 25.0%
! TGAAAATCAATATCTTGCAA+TGG + tig0019933:123845-123864 None:intergenic 25.0%
!!! ATTGCAAGATATTGATTTTC+AGG - tig0019933:123844-123863 Msa1377570:CDS 25.0%
! TGTATGAATTACTTTATCCT+GGG + tig0019933:123990-124009 None:intergenic 25.0%
! ATGTATGAATTACTTTATCC+TGG + tig0019933:123991-124010 None:intergenic 25.0%
!!! TTTGGTTTTAATTCTCAGTA+TGG - tig0019933:123023-123042 Msa1377570:CDS 25.0%
! GTTTAAATCAATATGAATCG+AGG - tig0019933:123102-123121 Msa1377570:CDS 25.0%
!!! AGAGTTTCTTCTATTGATTT+AGG - tig0019933:123530-123549 Msa1377570:CDS 25.0%
!! TGTTCCTAACATTTCTAAAA+TGG - tig0019933:123709-123728 Msa1377570:CDS 25.0%
!!! TTGTCCATTTTAGAAATGTT+AGG + tig0019933:123716-123735 None:intergenic 25.0%
!!! TAACATTTCTAAAATGGACA+AGG - tig0019933:123715-123734 Msa1377570:CDS 25.0%
! TATGGAAACTGAAAAAAACA+TGG - tig0019933:123796-123815 Msa1377570:CDS 25.0%
! TGAAAATCAATATCTTGCAA+TGG + tig0019933:123845-123864 None:intergenic 25.0%
!!! ATTGCAAGATATTGATTTTC+AGG - tig0019933:123844-123863 Msa1377570:CDS 25.0%
! TGTATGAATTACTTTATCCT+GGG + tig0019933:123990-124009 None:intergenic 25.0%
! ATGTATGAATTACTTTATCC+TGG + tig0019933:123991-124010 None:intergenic 25.0%
TTGTAAGAATGAAGAAGACA+AGG - tig0019933:122596-122615 Msa1377570:CDS 30.0%
TAAGAAATTGGACAGTGTTT+CGG - tig0019933:122962-122981 Msa1377570:CDS 30.0%
AAAAAACATGGCTGACAATA+AGG - tig0019933:123808-123827 Msa1377570:CDS 30.0%
AAAAACATGGCTGACAATAA+GGG - tig0019933:123809-123828 Msa1377570:CDS 30.0%
ACTTTAACAATTCCAGAAAC+CGG + tig0019933:123920-123939 None:intergenic 30.0%
TTGTAAGAATGAAGAAGACA+AGG - tig0019933:122596-122615 Msa1377570:CDS 30.0%
TAAGAAATTGGACAGTGTTT+CGG - tig0019933:122962-122981 Msa1377570:CDS 30.0%
AAAAAACATGGCTGACAATA+AGG - tig0019933:123808-123827 Msa1377570:CDS 30.0%
AAAAACATGGCTGACAATAA+GGG - tig0019933:123809-123828 Msa1377570:CDS 30.0%
ACTTTAACAATTCCAGAAAC+CGG + tig0019933:123920-123939 None:intergenic 30.0%
AGGTTGTGTCACATTGTTAA+CGG - tig0019933:122735-122754 Msa1377570:CDS 35.0%
TATGTGTTGAAATACGGTGA+GGG - tig0019933:122813-122832 Msa1377570:CDS 35.0%
AAAGGGAGAAAGATGATGTT+AGG - tig0019933:122877-122896 Msa1377570:CDS 35.0%
ACCTCTGTCTATTATGTGTT+TGG - tig0019933:123005-123024 Msa1377570:CDS 35.0%
ACCAAACACATAATAGACAG+AGG + tig0019933:123009-123028 None:intergenic 35.0%
TTTAATTCTCAGTATGGTCC+TGG - tig0019933:123029-123048 Msa1377570:CDS 35.0%
GCACACTTAAAAGAATTTCC+AGG + tig0019933:123050-123069 None:intergenic 35.0%
TTAAGTGTGCTAAACCAAGT+TGG - tig0019933:123060-123079 Msa1377570:CDS 35.0%
!!! AGAGCAAGGTTTTTTGGATA+TGG - tig0019933:123220-123239 Msa1377570:CDS 35.0%
!!! AAGGTTTTTTGGATATGGTC+AGG - tig0019933:123225-123244 Msa1377570:CDS 35.0%
!!! AGGTTTTTTGGATATGGTCA+GGG - tig0019933:123226-123245 Msa1377570:CDS 35.0%
! AAGGTAGCACCAAAGATTTT+TGG - tig0019933:123281-123300 Msa1377570:CDS 35.0%
! AGGTAGCACCAAAGATTTTT+GGG - tig0019933:123282-123301 Msa1377570:CDS 35.0%
! TATTAGTTTCTCCCCAAAAG+TGG - tig0019933:123346-123365 Msa1377570:CDS 35.0%
!! AGAATCATCTTCCACTTTTG+GGG + tig0019933:123360-123379 None:intergenic 35.0%
!! CAGAATCATCTTCCACTTTT+GGG + tig0019933:123361-123380 None:intergenic 35.0%
TGGAAGAATTACCATAAGCA+TGG + tig0019933:123427-123446 None:intergenic 35.0%
GCTTATGGTAATTCTTCCAA+CGG - tig0019933:123428-123447 Msa1377570:CDS 35.0%
CTTATGGTAATTCTTCCAAC+GGG - tig0019933:123429-123448 Msa1377570:CDS 35.0%
TTATGGTAATTCTTCCAACG+GGG - tig0019933:123430-123449 Msa1377570:CDS 35.0%
CTTTAACAATTCCAGAAACC+GGG + tig0019933:123919-123938 None:intergenic 35.0%
AGGTTGTGTCACATTGTTAA+CGG - tig0019933:122735-122754 Msa1377570:CDS 35.0%
TATGTGTTGAAATACGGTGA+GGG - tig0019933:122813-122832 Msa1377570:CDS 35.0%
AAAGGGAGAAAGATGATGTT+AGG - tig0019933:122877-122896 Msa1377570:CDS 35.0%
ACCTCTGTCTATTATGTGTT+TGG - tig0019933:123005-123024 Msa1377570:CDS 35.0%
ACCAAACACATAATAGACAG+AGG + tig0019933:123009-123028 None:intergenic 35.0%
TTTAATTCTCAGTATGGTCC+TGG - tig0019933:123029-123048 Msa1377570:CDS 35.0%
GCACACTTAAAAGAATTTCC+AGG + tig0019933:123050-123069 None:intergenic 35.0%
TTAAGTGTGCTAAACCAAGT+TGG - tig0019933:123060-123079 Msa1377570:CDS 35.0%
!!! AGAGCAAGGTTTTTTGGATA+TGG - tig0019933:123220-123239 Msa1377570:CDS 35.0%
!!! AAGGTTTTTTGGATATGGTC+AGG - tig0019933:123225-123244 Msa1377570:CDS 35.0%
!!! AGGTTTTTTGGATATGGTCA+GGG - tig0019933:123226-123245 Msa1377570:CDS 35.0%
! AAGGTAGCACCAAAGATTTT+TGG - tig0019933:123281-123300 Msa1377570:CDS 35.0%
! AGGTAGCACCAAAGATTTTT+GGG - tig0019933:123282-123301 Msa1377570:CDS 35.0%
! TATTAGTTTCTCCCCAAAAG+TGG - tig0019933:123346-123365 Msa1377570:CDS 35.0%
!! AGAATCATCTTCCACTTTTG+GGG + tig0019933:123360-123379 None:intergenic 35.0%
!! CAGAATCATCTTCCACTTTT+GGG + tig0019933:123361-123380 None:intergenic 35.0%
TGGAAGAATTACCATAAGCA+TGG + tig0019933:123427-123446 None:intergenic 35.0%
GCTTATGGTAATTCTTCCAA+CGG - tig0019933:123428-123447 Msa1377570:CDS 35.0%
CTTATGGTAATTCTTCCAAC+GGG - tig0019933:123429-123448 Msa1377570:CDS 35.0%
TTATGGTAATTCTTCCAACG+GGG - tig0019933:123430-123449 Msa1377570:CDS 35.0%
CTTTAACAATTCCAGAAACC+GGG + tig0019933:123919-123938 None:intergenic 35.0%
TGAAGAAGACAAGGCTATGT+TGG - tig0019933:122605-122624 Msa1377570:CDS 40.0%
GCTATGTTGGAATCTGTGTT+GGG - tig0019933:122618-122637 Msa1377570:CDS 40.0%
ATTCGGCGGAACAATAACAT+CGG + tig0019933:122694-122713 None:intergenic 40.0%
TGGATAGTCACGTCAAGATT+CGG + tig0019933:122711-122730 None:intergenic 40.0%
TTGACGTGACTATCCACAAA+AGG - tig0019933:122715-122734 Msa1377570:CDS 40.0%
! CAATGTGACACAACCTTTTG+TGG + tig0019933:122731-122750 None:intergenic 40.0%
ACATTGTTAACGGTTCCAAG+TGG - tig0019933:122745-122764 Msa1377570:CDS 40.0%
GTATTTCAACACATAGCCAC+CGG + tig0019933:122808-122827 None:intergenic 40.0%
GGTGGCTATGTGTTGAAATA+CGG - tig0019933:122807-122826 Msa1377570:CDS 40.0%
CTATGTGTTGAAATACGGTG+AGG - tig0019933:122812-122831 Msa1377570:CDS 40.0%
GAAAGATGATGTTAGGAGGA+AGG - tig0019933:122884-122903 Msa1377570:CDS 40.0%
! TGATGTTAGGAGGAAGGTTT+TGG - tig0019933:122890-122909 Msa1377570:CDS 40.0%
! GATGTTAGGAGGAAGGTTTT+GGG - tig0019933:122891-122910 Msa1377570:CDS 40.0%
! ATGTTAGGAGGAAGGTTTTG+GGG - tig0019933:122892-122911 Msa1377570:CDS 40.0%
!!! AAGGTTTTGGGGAAGATTCA+TGG - tig0019933:122903-122922 Msa1377570:CDS 40.0%
ATATGAATCGAGGTCGTTTC+TGG - tig0019933:123112-123131 Msa1377570:CDS 40.0%
! GCTTTTGTACCTCTTAAAGC+CGG - tig0019933:123161-123180 Msa1377570:CDS 40.0%
! CTTTTGTACCTCTTAAAGCC+GGG - tig0019933:123162-123181 Msa1377570:CDS 40.0%
! TTTTGTACCTCTTAAAGCCG+GGG - tig0019933:123163-123182 Msa1377570:CDS 40.0%
TCCAAAAAACCTTGCTCTTC+AGG + tig0019933:123218-123237 None:intergenic 40.0%
!! ACTTTTGGAGAATCTACCTC+TGG - tig0019933:123251-123270 Msa1377570:CDS 40.0%
AACTCGTGCCCAAAAATCTT+TGG + tig0019933:123293-123312 None:intergenic 40.0%
! CCAGAATCATCTTCCACTTT+TGG + tig0019933:123362-123381 None:intergenic 40.0%
CCAAAAGTGGAAGATGATTC+TGG - tig0019933:123359-123378 Msa1377570:CDS 40.0%
TATGGTAATTCTTCCAACGG+GGG - tig0019933:123431-123450 Msa1377570:CDS 40.0%
!! GCAAAAAAGTACCCTCAGTT+GGG - tig0019933:123473-123492 Msa1377570:CDS 40.0%
GAATCATTTGACCCAACTGA+GGG + tig0019933:123487-123506 None:intergenic 40.0%
AGTTGGGTCAAATGATTCCT+GGG - tig0019933:123489-123508 Msa1377570:CDS 40.0%
! TCCTGGGAATTTTACTTCTC+AGG - tig0019933:123505-123524 Msa1377570:CDS 40.0%
! GGTTTTCTCCCTCTTTTTCT+AGG + tig0019933:123596-123615 None:intergenic 40.0%
ACCACTGAATCATGTTGAAG+CGG - tig0019933:123634-123653 Msa1377570:CDS 40.0%
CTCTTAAGGCGTAAAATCTC+TGG + tig0019933:123685-123704 None:intergenic 40.0%
GTTAGGAACAACAGCTCTTA+AGG + tig0019933:123699-123718 None:intergenic 40.0%
! CTTCTGGAGATCAGTGATAT+GGG + tig0019933:123765-123784 None:intergenic 40.0%
! TCTTCTGGAGATCAGTGATA+TGG + tig0019933:123766-123785 None:intergenic 40.0%
CCATAAGCTTGATCTTCTTC+TGG + tig0019933:123781-123800 None:intergenic 40.0%
CCAGAAGAAGATCAAGCTTA+TGG - tig0019933:123778-123797 Msa1377570:CDS 40.0%
TATCTTGCAATGGCAATTGC+TGG + tig0019933:123835-123854 None:intergenic 40.0%
!!! TATTGATTTTCAGGCTCGAC+AGG - tig0019933:123853-123872 Msa1377570:CDS 40.0%
GCATCAAATTGTAGCTCAAG+AGG - tig0019933:123949-123968 Msa1377570:CDS 40.0%
ATCCTGGGAAGTTGAAACAT+TGG + tig0019933:123975-123994 None:intergenic 40.0%
ACATTCTCCATCAGAACTCA+AGG - tig0019933:124007-124026 Msa1377570:CDS 40.0%
TGAAGAAGACAAGGCTATGT+TGG - tig0019933:122605-122624 Msa1377570:CDS 40.0%
GCTATGTTGGAATCTGTGTT+GGG - tig0019933:122618-122637 Msa1377570:CDS 40.0%
ATTCGGCGGAACAATAACAT+CGG + tig0019933:122694-122713 None:intergenic 40.0%
TGGATAGTCACGTCAAGATT+CGG + tig0019933:122711-122730 None:intergenic 40.0%
TTGACGTGACTATCCACAAA+AGG - tig0019933:122715-122734 Msa1377570:CDS 40.0%
! CAATGTGACACAACCTTTTG+TGG + tig0019933:122731-122750 None:intergenic 40.0%
ACATTGTTAACGGTTCCAAG+TGG - tig0019933:122745-122764 Msa1377570:CDS 40.0%
GTATTTCAACACATAGCCAC+CGG + tig0019933:122808-122827 None:intergenic 40.0%
GGTGGCTATGTGTTGAAATA+CGG - tig0019933:122807-122826 Msa1377570:CDS 40.0%
CTATGTGTTGAAATACGGTG+AGG - tig0019933:122812-122831 Msa1377570:CDS 40.0%
GAAAGATGATGTTAGGAGGA+AGG - tig0019933:122884-122903 Msa1377570:CDS 40.0%
! TGATGTTAGGAGGAAGGTTT+TGG - tig0019933:122890-122909 Msa1377570:CDS 40.0%
! GATGTTAGGAGGAAGGTTTT+GGG - tig0019933:122891-122910 Msa1377570:CDS 40.0%
! ATGTTAGGAGGAAGGTTTTG+GGG - tig0019933:122892-122911 Msa1377570:CDS 40.0%
!!! AAGGTTTTGGGGAAGATTCA+TGG - tig0019933:122903-122922 Msa1377570:CDS 40.0%
ATATGAATCGAGGTCGTTTC+TGG - tig0019933:123112-123131 Msa1377570:CDS 40.0%
! GCTTTTGTACCTCTTAAAGC+CGG - tig0019933:123161-123180 Msa1377570:CDS 40.0%
! CTTTTGTACCTCTTAAAGCC+GGG - tig0019933:123162-123181 Msa1377570:CDS 40.0%
! TTTTGTACCTCTTAAAGCCG+GGG - tig0019933:123163-123182 Msa1377570:CDS 40.0%
TCCAAAAAACCTTGCTCTTC+AGG + tig0019933:123218-123237 None:intergenic 40.0%
!! ACTTTTGGAGAATCTACCTC+TGG - tig0019933:123251-123270 Msa1377570:CDS 40.0%
AACTCGTGCCCAAAAATCTT+TGG + tig0019933:123293-123312 None:intergenic 40.0%
! CCAGAATCATCTTCCACTTT+TGG + tig0019933:123362-123381 None:intergenic 40.0%
CCAAAAGTGGAAGATGATTC+TGG - tig0019933:123359-123378 Msa1377570:CDS 40.0%
TATGGTAATTCTTCCAACGG+GGG - tig0019933:123431-123450 Msa1377570:CDS 40.0%
!! GCAAAAAAGTACCCTCAGTT+GGG - tig0019933:123473-123492 Msa1377570:CDS 40.0%
GAATCATTTGACCCAACTGA+GGG + tig0019933:123487-123506 None:intergenic 40.0%
AGTTGGGTCAAATGATTCCT+GGG - tig0019933:123489-123508 Msa1377570:CDS 40.0%
! TCCTGGGAATTTTACTTCTC+AGG - tig0019933:123505-123524 Msa1377570:CDS 40.0%
! GGTTTTCTCCCTCTTTTTCT+AGG + tig0019933:123596-123615 None:intergenic 40.0%
ACCACTGAATCATGTTGAAG+CGG - tig0019933:123634-123653 Msa1377570:CDS 40.0%
CTCTTAAGGCGTAAAATCTC+TGG + tig0019933:123685-123704 None:intergenic 40.0%
GTTAGGAACAACAGCTCTTA+AGG + tig0019933:123699-123718 None:intergenic 40.0%
! CTTCTGGAGATCAGTGATAT+GGG + tig0019933:123765-123784 None:intergenic 40.0%
! TCTTCTGGAGATCAGTGATA+TGG + tig0019933:123766-123785 None:intergenic 40.0%
CCATAAGCTTGATCTTCTTC+TGG + tig0019933:123781-123800 None:intergenic 40.0%
CCAGAAGAAGATCAAGCTTA+TGG - tig0019933:123778-123797 Msa1377570:CDS 40.0%
TATCTTGCAATGGCAATTGC+TGG + tig0019933:123835-123854 None:intergenic 40.0%
!!! TATTGATTTTCAGGCTCGAC+AGG - tig0019933:123853-123872 Msa1377570:CDS 40.0%
GCATCAAATTGTAGCTCAAG+AGG - tig0019933:123949-123968 Msa1377570:CDS 40.0%
ATCCTGGGAAGTTGAAACAT+TGG + tig0019933:123975-123994 None:intergenic 40.0%
ACATTCTCCATCAGAACTCA+AGG - tig0019933:124007-124026 Msa1377570:CDS 40.0%
GGCTATGTTGGAATCTGTGT+TGG - tig0019933:122617-122636 Msa1377570:CDS 45.0%
ATAGTCACGTCAAGATTCGG+CGG + tig0019933:122708-122727 None:intergenic 45.0%
CAAAATCGCGTAGTTCCACT+TGG + tig0019933:122763-122782 None:intergenic 45.0%
! AGTGGAACTACGCGATTTTG+TGG - tig0019933:122763-122782 Msa1377570:CDS 45.0%
ATAGCCACCGGATTTCAAAC+CGG + tig0019933:122796-122815 None:intergenic 45.0%
TCCAAGGAATGAGCAGGAAA+GGG - tig0019933:122860-122879 Msa1377570:CDS 45.0%
GGGAGAAAGATGATGTTAGG+AGG - tig0019933:122880-122899 Msa1377570:CDS 45.0%
!! GAGGTCGTTTCTGGCTAAAT+CGG - tig0019933:123121-123140 Msa1377570:CDS 45.0%
!! TCTATAACCCCGGCTTTAAG+AGG + tig0019933:123173-123192 None:intergenic 45.0%
! GGTTATAGAGCTTGGTTCGA+CGG - tig0019933:123184-123203 Msa1377570:CDS 45.0%
!!! GCCTGAAGAGCAAGGTTTTT+TGG - tig0019933:123214-123233 Msa1377570:CDS 45.0%
! GATATGGTCAGGGCAACTTT+TGG - tig0019933:123236-123255 Msa1377570:CDS 45.0%
! TTTTGGGCACGAGTTAAGCT+TGG - tig0019933:123298-123317 Msa1377570:CDS 45.0%
TTTGGGCACGAGTTAAGCTT+GGG - tig0019933:123299-123318 Msa1377570:CDS 45.0%
TCTTACGAAGTACAAGCGCT+TGG - tig0019933:123395-123414 Msa1377570:CDS 45.0%
!! GGCAAAAAAGTACCCTCAGT+TGG - tig0019933:123472-123491 Msa1377570:CDS 45.0%
GGAATCATTTGACCCAACTG+AGG + tig0019933:123488-123507 None:intergenic 45.0%
CAGTTGGGTCAAATGATTCC+TGG - tig0019933:123488-123507 Msa1377570:CDS 45.0%
GCCTGAGAAGTAAAATTCCC+AGG + tig0019933:123509-123528 None:intergenic 45.0%
AGCGGAAGCCTAGAAAAAGA+GGG - tig0019933:123585-123604 Msa1377570:CDS 45.0%
TCCGCTTCAACATGATTCAG+TGG + tig0019933:123638-123657 None:intergenic 45.0%
TGAATCATGTTGAAGCGGAG+AGG - tig0019933:123639-123658 Msa1377570:CDS 45.0%
ATGGACAAGGCTTCTCTTCT+TGG - tig0019933:123728-123747 Msa1377570:CDS 45.0%
TAACAATTCCAGAAACCGGG+TGG + tig0019933:123916-123935 None:intergenic 45.0%
TTCTCCATCAGAACTCAAGG+AGG - tig0019933:124010-124029 Msa1377570:CDS 45.0%
TCTCCATCAGAACTCAAGGA+GGG - tig0019933:124011-124030 Msa1377570:CDS 45.0%
GGCTATGTTGGAATCTGTGT+TGG - tig0019933:122617-122636 Msa1377570:CDS 45.0%
ATAGTCACGTCAAGATTCGG+CGG + tig0019933:122708-122727 None:intergenic 45.0%
CAAAATCGCGTAGTTCCACT+TGG + tig0019933:122763-122782 None:intergenic 45.0%
! AGTGGAACTACGCGATTTTG+TGG - tig0019933:122763-122782 Msa1377570:CDS 45.0%
ATAGCCACCGGATTTCAAAC+CGG + tig0019933:122796-122815 None:intergenic 45.0%
TCCAAGGAATGAGCAGGAAA+GGG - tig0019933:122860-122879 Msa1377570:CDS 45.0%
GGGAGAAAGATGATGTTAGG+AGG - tig0019933:122880-122899 Msa1377570:CDS 45.0%
!! GAGGTCGTTTCTGGCTAAAT+CGG - tig0019933:123121-123140 Msa1377570:CDS 45.0%
!! TCTATAACCCCGGCTTTAAG+AGG + tig0019933:123173-123192 None:intergenic 45.0%
! GGTTATAGAGCTTGGTTCGA+CGG - tig0019933:123184-123203 Msa1377570:CDS 45.0%
!!! GCCTGAAGAGCAAGGTTTTT+TGG - tig0019933:123214-123233 Msa1377570:CDS 45.0%
! GATATGGTCAGGGCAACTTT+TGG - tig0019933:123236-123255 Msa1377570:CDS 45.0%
! TTTTGGGCACGAGTTAAGCT+TGG - tig0019933:123298-123317 Msa1377570:CDS 45.0%
TTTGGGCACGAGTTAAGCTT+GGG - tig0019933:123299-123318 Msa1377570:CDS 45.0%
TCTTACGAAGTACAAGCGCT+TGG - tig0019933:123395-123414 Msa1377570:CDS 45.0%
!! GGCAAAAAAGTACCCTCAGT+TGG - tig0019933:123472-123491 Msa1377570:CDS 45.0%
GGAATCATTTGACCCAACTG+AGG + tig0019933:123488-123507 None:intergenic 45.0%
CAGTTGGGTCAAATGATTCC+TGG - tig0019933:123488-123507 Msa1377570:CDS 45.0%
GCCTGAGAAGTAAAATTCCC+AGG + tig0019933:123509-123528 None:intergenic 45.0%
AGCGGAAGCCTAGAAAAAGA+GGG - tig0019933:123585-123604 Msa1377570:CDS 45.0%
TCCGCTTCAACATGATTCAG+TGG + tig0019933:123638-123657 None:intergenic 45.0%
TGAATCATGTTGAAGCGGAG+AGG - tig0019933:123639-123658 Msa1377570:CDS 45.0%
ATGGACAAGGCTTCTCTTCT+TGG - tig0019933:123728-123747 Msa1377570:CDS 45.0%
TAACAATTCCAGAAACCGGG+TGG + tig0019933:123916-123935 None:intergenic 45.0%
TTCTCCATCAGAACTCAAGG+AGG - tig0019933:124010-124029 Msa1377570:CDS 45.0%
TCTCCATCAGAACTCAAGGA+GGG - tig0019933:124011-124030 Msa1377570:CDS 45.0%
! GAACTACGCGATTTTGTGGC+AGG - tig0019933:122767-122786 Msa1377570:CDS 50.0%
!! CAGGTTGCCGGTTTGAAATC+CGG - tig0019933:122786-122805 Msa1377570:CDS 50.0%
GACACTGTCAGGATCCCAAA+GGG - tig0019933:122835-122854 Msa1377570:CDS 50.0%
ACACTGTCAGGATCCCAAAG+GGG - tig0019933:122836-122855 Msa1377570:CDS 50.0%
TCATTCCTTGGACCCCCTTT+GGG + tig0019933:122852-122871 None:intergenic 50.0%
GTCCAAGGAATGAGCAGGAA+AGG - tig0019933:122859-122878 Msa1377570:CDS 50.0%
TCCCTTTCCTGCTCATTCCT+TGG + tig0019933:122864-122883 None:intergenic 50.0%
TGGGGAAGATTCATGGTTGC+TGG - tig0019933:122910-122929 Msa1377570:CDS 50.0%
GGGGAAGATTCATGGTTGCT+GGG - tig0019933:122911-122930 Msa1377570:CDS 50.0%
GGGAAGATTCATGGTTGCTG+GGG - tig0019933:122912-122931 Msa1377570:CDS 50.0%
AAGATTCATGGTTGCTGGGG+TGG - tig0019933:122915-122934 Msa1377570:CDS 50.0%
CCAGCATCAGAAGACCAACT+TGG + tig0019933:123077-123096 None:intergenic 50.0%
!! CCAAGTTGGTCTTCTGATGC+TGG - tig0019933:123074-123093 Msa1377570:CDS 50.0%
!! TCGTTTCTGGCTAAATCGGC+TGG - tig0019933:123125-123144 Msa1377570:CDS 50.0%
AAAGCCGGGGTTATAGAGCT+TGG - tig0019933:123176-123195 Msa1377570:CDS 50.0%
CGAACCAAGCTCTATAACCC+CGG + tig0019933:123183-123202 None:intergenic 50.0%
TGGAGAATCTACCTCTGGAC+AGG - tig0019933:123256-123275 Msa1377570:CDS 50.0%
ATCTACCTCTGGACAGGCTA+AGG - tig0019933:123262-123281 Msa1377570:CDS 50.0%
TTGGGCACGAGTTAAGCTTG+GGG - tig0019933:123300-123319 Msa1377570:CDS 50.0%
CTTGGCGCAAACCATGCTTA+TGG - tig0019933:123413-123432 Msa1377570:CDS 50.0%
! AATTCTTCCAACGGGGGTGT+AGG - tig0019933:123437-123456 Msa1377570:CDS 50.0%
GAGCGGAAGCCTAGAAAAAG+AGG - tig0019933:123584-123603 Msa1377570:CDS 50.0%
AGAGGGAGAAAACCTGCCAA+TGG - tig0019933:123602-123621 Msa1377570:CDS 50.0%
AAAACCTGCCAATGGACGAG+AGG - tig0019933:123610-123629 Msa1377570:CDS 50.0%
TTGAAGCGGAGAGGCAAAGA+CGG - tig0019933:123648-123667 Msa1377570:CDS 50.0%
TGTTAGAGTTAGCTGCCCCT+TGG - tig0019933:123886-123905 Msa1377570:CDS 50.0%
AAACCGGGTGGATATCCAAG+GGG + tig0019933:123904-123923 None:intergenic 50.0%
GAAACCGGGTGGATATCCAA+GGG + tig0019933:123905-123924 None:intergenic 50.0%
AGAAACCGGGTGGATATCCA+AGG + tig0019933:123906-123925 None:intergenic 50.0%
TTGGATATCCACCCGGTTTC+TGG - tig0019933:123905-123924 Msa1377570:CDS 50.0%
GGCCAATGTTTCAACTTCCC+AGG - tig0019933:123970-123989 Msa1377570:CDS 50.0%
CTCCATCAGAACTCAAGGAG+GGG - tig0019933:124012-124031 Msa1377570:CDS 50.0%
! TTCCCCTCCTTGAGTTCTGA+TGG + tig0019933:124017-124036 None:intergenic 50.0%
! GAACTACGCGATTTTGTGGC+AGG - tig0019933:122767-122786 Msa1377570:CDS 50.0%
!! CAGGTTGCCGGTTTGAAATC+CGG - tig0019933:122786-122805 Msa1377570:CDS 50.0%
GACACTGTCAGGATCCCAAA+GGG - tig0019933:122835-122854 Msa1377570:CDS 50.0%
ACACTGTCAGGATCCCAAAG+GGG - tig0019933:122836-122855 Msa1377570:CDS 50.0%
TCATTCCTTGGACCCCCTTT+GGG + tig0019933:122852-122871 None:intergenic 50.0%
GTCCAAGGAATGAGCAGGAA+AGG - tig0019933:122859-122878 Msa1377570:CDS 50.0%
TCCCTTTCCTGCTCATTCCT+TGG + tig0019933:122864-122883 None:intergenic 50.0%
TGGGGAAGATTCATGGTTGC+TGG - tig0019933:122910-122929 Msa1377570:CDS 50.0%
GGGGAAGATTCATGGTTGCT+GGG - tig0019933:122911-122930 Msa1377570:CDS 50.0%
GGGAAGATTCATGGTTGCTG+GGG - tig0019933:122912-122931 Msa1377570:CDS 50.0%
AAGATTCATGGTTGCTGGGG+TGG - tig0019933:122915-122934 Msa1377570:CDS 50.0%
CCAGCATCAGAAGACCAACT+TGG + tig0019933:123077-123096 None:intergenic 50.0%
!! CCAAGTTGGTCTTCTGATGC+TGG - tig0019933:123074-123093 Msa1377570:CDS 50.0%
!! TCGTTTCTGGCTAAATCGGC+TGG - tig0019933:123125-123144 Msa1377570:CDS 50.0%
AAAGCCGGGGTTATAGAGCT+TGG - tig0019933:123176-123195 Msa1377570:CDS 50.0%
CGAACCAAGCTCTATAACCC+CGG + tig0019933:123183-123202 None:intergenic 50.0%
TGGAGAATCTACCTCTGGAC+AGG - tig0019933:123256-123275 Msa1377570:CDS 50.0%
ATCTACCTCTGGACAGGCTA+AGG - tig0019933:123262-123281 Msa1377570:CDS 50.0%
TTGGGCACGAGTTAAGCTTG+GGG - tig0019933:123300-123319 Msa1377570:CDS 50.0%
CTTGGCGCAAACCATGCTTA+TGG - tig0019933:123413-123432 Msa1377570:CDS 50.0%
! AATTCTTCCAACGGGGGTGT+AGG - tig0019933:123437-123456 Msa1377570:CDS 50.0%
GAGCGGAAGCCTAGAAAAAG+AGG - tig0019933:123584-123603 Msa1377570:CDS 50.0%
AGAGGGAGAAAACCTGCCAA+TGG - tig0019933:123602-123621 Msa1377570:CDS 50.0%
AAAACCTGCCAATGGACGAG+AGG - tig0019933:123610-123629 Msa1377570:CDS 50.0%
TTGAAGCGGAGAGGCAAAGA+CGG - tig0019933:123648-123667 Msa1377570:CDS 50.0%
TGTTAGAGTTAGCTGCCCCT+TGG - tig0019933:123886-123905 Msa1377570:CDS 50.0%
AAACCGGGTGGATATCCAAG+GGG + tig0019933:123904-123923 None:intergenic 50.0%
GAAACCGGGTGGATATCCAA+GGG + tig0019933:123905-123924 None:intergenic 50.0%
AGAAACCGGGTGGATATCCA+AGG + tig0019933:123906-123925 None:intergenic 50.0%
TTGGATATCCACCCGGTTTC+TGG - tig0019933:123905-123924 Msa1377570:CDS 50.0%
GGCCAATGTTTCAACTTCCC+AGG - tig0019933:123970-123989 Msa1377570:CDS 50.0%
CTCCATCAGAACTCAAGGAG+GGG - tig0019933:124012-124031 Msa1377570:CDS 50.0%
! TTCCCCTCCTTGAGTTCTGA+TGG + tig0019933:124017-124036 None:intergenic 50.0%
! GCGATTTTGTGGCAGGTTGC+CGG - tig0019933:122774-122793 Msa1377570:CDS 55.0%
!! GTTGCCGGTTTGAAATCCGG+TGG - tig0019933:122789-122808 Msa1377570:CDS 55.0%
ATACGGTGAGGGACACTGTC+AGG - tig0019933:122824-122843 Msa1377570:CDS 55.0%
GGACACTGTCAGGATCCCAA+AGG - tig0019933:122834-122853 Msa1377570:CDS 55.0%
CACTGTCAGGATCCCAAAGG+GGG - tig0019933:122837-122856 Msa1377570:CDS 55.0%
CTCATTCCTTGGACCCCCTT+TGG + tig0019933:122853-122872 None:intergenic 55.0%
! AGAGCTTGGTTCGACGGAGA+TGG - tig0019933:123190-123209 Msa1377570:CDS 55.0%
GAGATGGTGCCTGAAGAGCA+AGG - tig0019933:123206-123225 Msa1377570:CDS 55.0%
TGCTACCTTAGCCTGTCCAG+AGG + tig0019933:123270-123289 None:intergenic 55.0%
TGGGCACGAGTTAAGCTTGG+GGG - tig0019933:123301-123320 Msa1377570:CDS 55.0%
GAAGACTCCTCCTCACCTCT+TGG - tig0019933:123557-123576 Msa1377570:CDS 55.0%
CAGTGGTTCCTCTCGTCCAT+TGG + tig0019933:123621-123640 None:intergenic 55.0%
TGAAGCGGAGAGGCAAAGAC+GGG - tig0019933:123649-123668 Msa1377570:CDS 55.0%
! GCGATTTTGTGGCAGGTTGC+CGG - tig0019933:122774-122793 Msa1377570:CDS 55.0%
!! GTTGCCGGTTTGAAATCCGG+TGG - tig0019933:122789-122808 Msa1377570:CDS 55.0%
ATACGGTGAGGGACACTGTC+AGG - tig0019933:122824-122843 Msa1377570:CDS 55.0%
GGACACTGTCAGGATCCCAA+AGG - tig0019933:122834-122853 Msa1377570:CDS 55.0%
CACTGTCAGGATCCCAAAGG+GGG - tig0019933:122837-122856 Msa1377570:CDS 55.0%
CTCATTCCTTGGACCCCCTT+TGG + tig0019933:122853-122872 None:intergenic 55.0%
! AGAGCTTGGTTCGACGGAGA+TGG - tig0019933:123190-123209 Msa1377570:CDS 55.0%
GAGATGGTGCCTGAAGAGCA+AGG - tig0019933:123206-123225 Msa1377570:CDS 55.0%
TGCTACCTTAGCCTGTCCAG+AGG + tig0019933:123270-123289 None:intergenic 55.0%
TGGGCACGAGTTAAGCTTGG+GGG - tig0019933:123301-123320 Msa1377570:CDS 55.0%
GAAGACTCCTCCTCACCTCT+TGG - tig0019933:123557-123576 Msa1377570:CDS 55.0%
CAGTGGTTCCTCTCGTCCAT+TGG + tig0019933:123621-123640 None:intergenic 55.0%
TGAAGCGGAGAGGCAAAGAC+GGG - tig0019933:123649-123668 Msa1377570:CDS 55.0%
AGGATCCCAAAGGGGGTCCA+AGG - tig0019933:122844-122863 Msa1377570:CDS 60.0%
AGGGGGTCCAAGGAATGAGC+AGG - tig0019933:122854-122873 Msa1377570:CDS 60.0%
GGGTGTAGGAGACAGCAACG+AGG - tig0019933:123451-123470 Msa1377570:CDS 60.0%
CTCGTCTCCAAGAGGTGAGG+AGG + tig0019933:123567-123586 None:intergenic 60.0%
CCTCACCTCTTGGAGACGAG+CGG - tig0019933:123567-123586 Msa1377570:CDS 60.0%
GGTTCCTCTCGTCCATTGGC+AGG + tig0019933:123617-123636 None:intergenic 60.0%
CTGCCCCTTGGATATCCACC+CGG - tig0019933:123898-123917 Msa1377570:CDS 60.0%
AGGATCCCAAAGGGGGTCCA+AGG - tig0019933:122844-122863 Msa1377570:CDS 60.0%
AGGGGGTCCAAGGAATGAGC+AGG - tig0019933:122854-122873 Msa1377570:CDS 60.0%
GGGTGTAGGAGACAGCAACG+AGG - tig0019933:123451-123470 Msa1377570:CDS 60.0%
CTCGTCTCCAAGAGGTGAGG+AGG + tig0019933:123567-123586 None:intergenic 60.0%
CCTCACCTCTTGGAGACGAG+CGG - tig0019933:123567-123586 Msa1377570:CDS 60.0%
GGTTCCTCTCGTCCATTGGC+AGG + tig0019933:123617-123636 None:intergenic 60.0%
CTGCCCCTTGGATATCCACC+CGG - tig0019933:123898-123917 Msa1377570:CDS 60.0%
GCTGTCTCCTACACCCCCGT+TGG + tig0019933:123447-123466 None:intergenic 65.0%
CCGCTCGTCTCCAAGAGGTG+AGG + tig0019933:123570-123589 None:intergenic 65.0%
GGCTTCCGCTCGTCTCCAAG+AGG + tig0019933:123575-123594 None:intergenic 65.0%
GCTGTCTCCTACACCCCCGT+TGG + tig0019933:123447-123466 None:intergenic 65.0%
CCGCTCGTCTCCAAGAGGTG+AGG + tig0019933:123570-123589 None:intergenic 65.0%
GGCTTCCGCTCGTCTCCAAG+AGG + tig0019933:123575-123594 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0019933 gene 122579 124090 122579 ID=Msa1377570;Name=Msa1377570
tig0019933 mRNA 122579 124090 122579 ID=Msa1377570-mRNA-1;Parent=Msa1377570;Name=Msa1377570-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|-1|0|1|-1|1|1|0|503
tig0019933 exon 122579 124090 122579 ID=Msa1377570-mRNA-1:exon:5696;Parent=Msa1377570-mRNA-1
tig0019933 CDS 122579 124090 122579 ID=Msa1377570-mRNA-1:cds;Parent=Msa1377570-mRNA-1
Gene Sequence

>Msa1377570

ATGGCTGGTGAGAGGTTTTGTAAGAATGAAGAAGACAAGGCTATGTTGGAATCTGTGTTGGGTAATGAAGCTTTTGCATTTTTTAGCTCAGCTGTTTCAAAACATGTTTTTTCCGATGTTATTGTTCCGCCGAATCTTGACGTGACTATCCACAAAAGGTTGTGTCACATTGTTAACGGTTCCAAGTGGAACTACGCGATTTTGTGGCAGGTTGCCGGTTTGAAATCCGGTGGCTATGTGTTGAAATACGGTGAGGGACACTGTCAGGATCCCAAAGGGGGTCCAAGGAATGAGCAGGAAAGGGAGAAAGATGATGTTAGGAGGAAGGTTTTGGGGAAGATTCATGGTTGCTGGGGTGGATCTAGCTCAGTTGAGAATATTTATAAGAAATTGGACAGTGTTTCGGATTTGTATCTGCTTTACTTGACCTCTGTCTATTATGTGTTTGGTTTTAATTCTCAGTATGGTCCTGGAAATTCTTTTAAGTGTGCTAAACCAAGTTGGTCTTCTGATGCTGGTAGTTGTTTAAATCAATATGAATCGAGGTCGTTTCTGGCTAAATCGGCTGGTTTTCAAAGTGTTGCTTTTGTACCTCTTAAAGCCGGGGTTATAGAGCTTGGTTCGACGGAGATGGTGCCTGAAGAGCAAGGTTTTTTGGATATGGTCAGGGCAACTTTTGGAGAATCTACCTCTGGACAGGCTAAGGTAGCACCAAAGATTTTTGGGCACGAGTTAAGCTTGGGGGATACAAAATCTCAGTCGATAACTATTAGTTTCTCCCCAAAAGTGGAAGATGATTCTGGTTTCACTTCTGACTCTTACGAAGTACAAGCGCTTGGCGCAAACCATGCTTATGGTAATTCTTCCAACGGGGGTGTAGGAGACAGCAACGAGGCAAAAAAGTACCCTCAGTTGGGTCAAATGATTCCTGGGAATTTTACTTCTCAGGCAAGAGTTTCTTCTATTGATTTAGGTAATGAAGACTCCTCCTCACCTCTTGGAGACGAGCGGAAGCCTAGAAAAAGAGGGAGAAAACCTGCCAATGGACGAGAGGAACCACTGAATCATGTTGAAGCGGAGAGGCAAAGACGGGAGAAGCTTAACCAGAGATTTTACGCCTTAAGAGCTGTTGTTCCTAACATTTCTAAAATGGACAAGGCTTCTCTTCTTGGTGATGCTATTACCCATATCACTGATCTCCAGAAGAAGATCAAGCTTATGGAAACTGAAAAAAACATGGCTGACAATAAGGGCCAGCAATTGCCATTGCAAGATATTGATTTTCAGGCTCGACAGGATGATGCAGTTGTTAGAGTTAGCTGCCCCTTGGATATCCACCCGGTTTCTGGAATTGTTAAAGTGTTCAGAGAGCATCAAATTGTAGCTCAAGAGGCCAATGTTTCAACTTCCCAGGATAAAGTAATTCATACATTCTCCATCAGAACTCAAGGAGGGGAAGCAGCTGCTATACAGTTGAAAGAAAAGCTTGAAGCATCTTTAGCCAAAAATTAA
Protein sequence

>Msa1377570

MAGERFCKNEEDKAMLESVLGNEAFAFFSSAVSKHVFSDVIVPPNLDVTIHKRLCHIVNGSKWNYAILWQVAGLKSGGYVLKYGEGHCQDPKGGPRNEQEREKDDVRRKVLGKIHGCWGGSSSVENIYKKLDSVSDLYLLYLTSVYYVFGFNSQYGPGNSFKCAKPSWSSDAGSCLNQYESRSFLAKSAGFQSVAFVPLKAGVIELGSTEMVPEEQGFLDMVRATFGESTSGQAKVAPKIFGHELSLGDTKSQSITISFSPKVEDDSGFTSDSYEVQALGANHAYGNSSNGGVGDSNEAKKYPQLGQMIPGNFTSQARVSSIDLGNEDSSSPLGDERKPRKRGRKPANGREEPLNHVEAERQRREKLNQRFYALRAVVPNISKMDKASLLGDAITHITDLQKKIKLMETEKNMADNKGQQLPLQDIDFQARQDDAVVRVSCPLDIHPVSGIVKVFREHQIVAQEANVSTSQDKVIHTFSIRTQGGEAAAIQLKEKLEASLAKN*