AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1376130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1376130 MtrunA17_Chr7g0260841 98.012 503 10 0 1 503 1 503 0.0 1027
Msa1376130 MtrunA17_Chr7g0275821 54.348 184 65 3 335 502 232 412 9.09e-51 179
Msa1376130 MtrunA17_Chr7g0275821 30.387 181 94 7 5 158 6 181 1.07e-14 76.3
Msa1376130 MtrunA17_Chr8g0346161 28.306 484 242 15 43 481 19 442 1.75e-42 157
Msa1376130 MtrunA17_Chr5g0411341 42.584 209 81 3 328 501 466 670 9.84e-40 152
Msa1376130 MtrunA17_Chr5g0411341 25.967 181 126 4 49 223 44 222 2.37e-11 66.2
Msa1376130 MtrunA17_Chr8g0346151 27.571 457 220 10 63 475 38 427 4.52e-38 145
Msa1376130 MtrunA17_Chr8g0366751 43.575 179 66 3 337 480 444 622 3.72e-37 145
Msa1376130 MtrunA17_Chr8g0366751 28.804 184 123 6 49 226 53 234 1.09e-12 70.5
Msa1376130 MtrunA17_Chr4g0049331 56.250 64 28 0 353 416 133 196 7.66e-17 81.3
Msa1376130 MtrunA17_Chr5g0400661 46.591 88 42 2 333 416 123 209 3.00e-16 80.1
Msa1376130 MtrunA17_Chr1g0185621 42.857 98 51 2 326 419 443 539 2.19e-14 75.9
Msa1376130 MtrunA17_Chr1g0211081 43.182 88 49 1 324 410 285 372 3.41e-14 75.1
Msa1376130 MtrunA17_Chr4g0054561 46.341 82 38 2 336 417 140 215 4.32e-14 73.6
Msa1376130 MtrunA17_Chr4g0033391 31.765 170 77 4 245 408 64 200 6.12e-14 72.8
Msa1376130 MtrunA17_Chr2g0280161 54.386 57 26 0 355 411 177 233 9.35e-14 72.8
Msa1376130 MtrunA17_Chr2g0298751 42.308 104 46 4 356 445 53 156 1.40e-13 70.5
Msa1376130 MtrunA17_Chr4g0054571 53.704 54 25 0 355 408 150 203 3.98e-13 70.5
Msa1376130 MtrunA17_Chr2g0307131 38.182 110 55 3 299 408 136 232 4.38e-13 70.5
Msa1376130 MtrunA17_Chr5g0411331 27.957 186 116 6 49 223 27 205 7.41e-13 68.6
Msa1376130 MtrunA17_Chr4g0033281 37.113 97 51 1 312 408 120 206 7.67e-13 69.7
Msa1376130 MtrunA17_Chr4g0054551 38.462 104 56 3 341 441 138 236 1.42e-12 68.9
Msa1376130 MtrunA17_Chr3g0131691 53.448 58 27 0 353 410 296 353 2.34e-12 68.9
Msa1376130 MtrunA17_Chr5g0400671 32.231 121 57 2 342 441 145 261 2.57e-12 68.2
Msa1376130 MtrunA17_Chr7g0225901 40.625 96 51 3 313 408 52 141 2.64e-12 67.0
Msa1376130 MtrunA17_Chr8g0365711 22.129 357 177 12 188 496 111 414 2.76e-12 68.6
Msa1376130 MtrunA17_Chr4g0054581 45.588 68 31 1 341 408 190 251 3.53e-12 68.2
Msa1376130 MtrunA17_Chr5g0400621 43.836 73 39 1 338 410 142 212 3.80e-12 67.4
Msa1376130 MtrunA17_Chr7g0252041 51.724 58 28 0 353 410 284 341 4.48e-12 68.2
Msa1376130 MtrunA17_Chr2g0333621 35.652 115 66 4 308 418 90 200 6.58e-12 66.6
Msa1376130 MtrunA17_Chr8g0386921 50.820 61 30 0 351 411 402 462 6.77e-12 67.8
Msa1376130 MtrunA17_Chr4g0033291 30.488 164 95 3 245 408 64 208 7.15e-12 66.6
Msa1376130 MtrunA17_Chr5g0400641 50.000 52 26 0 359 410 2 53 8.73e-12 63.5
Msa1376130 MtrunA17_Chr6g0468461 51.852 54 26 0 355 408 173 226 1.49e-11 65.9
Msa1376130 MtrunA17_Chr4g0054621 33.884 121 67 2 297 408 109 225 2.08e-11 65.5
Msa1376130 MtrunA17_Chr2g0307141 51.852 54 26 0 355 408 183 236 2.45e-11 65.5
Msa1376130 MtrunA17_Chr2g0333641 32.031 128 82 3 308 433 89 213 3.14e-11 64.7
Msa1376130 MtrunA17_Chr4g0028071 43.939 66 34 1 345 410 142 204 6.73e-11 63.9
Msa1376130 MtrunA17_Chr2g0320291 28.000 150 86 4 359 486 137 286 7.11e-11 63.5
Msa1376130 MtrunA17_Chr6g0468491 50.000 54 27 0 355 408 137 190 8.75e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1376130 AT4G16430.1 47.431 506 220 11 3 502 2 467 5.53e-159 460
Msa1376130 AT2G46510.1 35.000 560 272 14 9 500 9 544 2.34e-93 295
Msa1376130 AT1G01260.1 52.096 167 72 3 338 502 416 576 1.21e-47 174
Msa1376130 AT1G01260.1 36.431 269 138 8 5 245 5 268 1.06e-40 155
Msa1376130 AT1G01260.2 52.096 167 72 3 338 502 416 576 1.21e-47 174
Msa1376130 AT1G01260.2 36.431 269 138 8 5 245 5 268 1.06e-40 155
Msa1376130 AT1G01260.3 52.096 167 72 3 338 502 416 576 1.21e-47 174
Msa1376130 AT1G01260.3 36.431 269 138 8 5 245 5 268 1.06e-40 155
Msa1376130 AT4G17880.1 27.843 510 286 13 49 481 62 566 5.06e-45 167
Msa1376130 AT1G32640.1 42.105 190 81 2 336 500 433 618 1.05e-36 144
Msa1376130 AT5G46760.1 40.816 196 88 1 336 503 396 591 3.99e-36 142
Msa1376130 AT5G46830.1 28.364 483 290 15 41 481 23 491 4.71e-35 137
Msa1376130 AT4G00870.1 37.059 170 73 5 333 468 220 389 1.76e-20 94.4
Msa1376130 AT4G00480.3 24.742 388 245 13 50 409 23 391 3.83e-20 94.0
Msa1376130 AT4G00480.1 24.742 388 245 13 50 409 23 391 4.33e-20 93.6
Msa1376130 AT4G00480.2 24.742 388 245 13 50 409 23 391 4.85e-20 94.0
Msa1376130 AT4G29930.6 27.419 186 111 3 327 493 31 211 2.24e-15 75.9
Msa1376130 AT2G16910.1 27.881 269 147 9 195 422 121 383 2.88e-15 79.0
Msa1376130 AT4G29930.2 26.776 183 113 4 327 493 31 208 6.71e-15 74.3
Msa1376130 AT5G57150.5 46.512 86 33 1 325 410 37 109 8.14e-15 74.3
Msa1376130 AT5G57150.3 46.512 86 33 1 325 410 38 110 9.07e-15 73.9
Msa1376130 AT5G57150.2 46.512 86 33 1 325 410 38 110 9.70e-15 73.9
Msa1376130 AT5G57150.1 46.512 86 33 1 325 410 37 109 1.44e-14 73.9
Msa1376130 AT5G57150.4 46.512 86 33 1 325 410 38 110 1.75e-14 73.9
Msa1376130 AT4G37850.2 49.333 75 38 0 337 411 134 208 2.64e-14 74.3
Msa1376130 AT4G37850.1 49.333 75 38 0 337 411 134 208 2.64e-14 74.3
Msa1376130 AT1G63650.3 47.761 67 35 0 351 417 401 467 5.96e-14 74.7
Msa1376130 AT1G63650.3 26.961 204 126 4 42 229 9 205 3.00e-13 72.8
Msa1376130 AT1G63650.1 47.761 67 35 0 351 417 401 467 5.96e-14 74.7
Msa1376130 AT1G63650.1 26.961 204 126 4 42 229 9 205 3.00e-13 72.8
Msa1376130 AT1G63650.2 47.761 67 35 0 351 417 401 467 5.96e-14 74.7
Msa1376130 AT1G63650.2 26.961 204 126 4 42 229 9 205 3.00e-13 72.8
Msa1376130 AT5G41315.3 25.446 224 125 6 42 238 15 223 1.18e-13 73.9
Msa1376130 AT5G41315.3 39.130 69 42 0 349 417 440 508 1.56e-11 67.4
Msa1376130 AT5G41315.2 25.446 224 125 6 42 238 18 226 1.28e-13 73.9
Msa1376130 AT5G41315.2 39.130 69 42 0 349 417 443 511 1.58e-11 67.4
Msa1376130 AT5G41315.1 25.446 224 125 6 42 238 10 218 1.32e-13 73.9
Msa1376130 AT5G41315.1 39.130 69 42 0 349 417 435 503 1.55e-11 67.4
Msa1376130 AT4G09820.1 27.108 166 106 2 335 496 354 508 1.95e-13 73.2
Msa1376130 AT4G29930.3 38.835 103 56 2 327 427 31 128 2.08e-13 70.9
Msa1376130 AT4G29930.7 38.835 103 56 2 327 427 31 128 2.08e-13 70.9
Msa1376130 AT4G29930.5 26.901 171 104 4 327 481 31 196 2.39e-13 70.1
Msa1376130 AT4G29930.1 38.835 103 56 2 327 427 31 128 2.57e-13 70.5
Msa1376130 AT4G29930.4 38.835 103 56 2 327 427 31 128 2.63e-13 68.9
Msa1376130 AT2G22750.2 51.562 64 31 0 341 404 112 175 4.11e-13 70.5
Msa1376130 AT2G22750.4 51.562 64 31 0 341 404 140 203 4.14e-13 70.9
Msa1376130 AT2G22750.1 51.562 64 31 0 341 404 112 175 4.14e-13 70.5
Msa1376130 AT2G22750.3 51.562 64 31 0 341 404 140 203 4.17e-13 70.9
Msa1376130 AT2G22750.5 51.562 64 31 0 341 404 136 199 4.26e-13 70.9
Msa1376130 AT1G12860.2 55.172 58 26 0 353 410 265 322 5.75e-13 71.2
Msa1376130 AT1G12860.1 55.172 58 26 0 353 410 265 322 6.64e-13 71.2
Msa1376130 AT2G22770.1 44.872 78 40 1 349 423 123 200 1.34e-12 69.3
Msa1376130 AT3G26744.1 53.448 58 27 0 353 410 305 362 2.35e-12 69.7
Msa1376130 AT3G26744.4 53.448 58 27 0 353 410 305 362 2.35e-12 69.7
Msa1376130 AT3G26744.2 53.448 58 27 0 353 410 305 362 2.35e-12 69.7
Msa1376130 AT3G26744.6 53.448 58 27 0 353 410 305 362 2.41e-12 69.3
Msa1376130 AT5G10570.1 36.220 127 70 3 299 416 89 213 6.68e-11 63.9

Find 121 sgRNAs with CRISPR-Local

Find 324 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGATATCCACCCGGTTTC+TGG 0.132043 tig0019784:-82632 Msa1376130:CDS
ATGGACAAGGCTTCTCTTCT+TGG 0.203486 tig0019784:-82809 Msa1376130:CDS
CCAGAATCATCTTCCACTTT+TGG 0.206725 tig0019784:+83178 None:intergenic
TGAGAATATTTATAAGAAAT+TGG 0.206945 tig0019784:-83587 Msa1376130:CDS
TGTTCCTAACATTTCTAAAA+TGG 0.208602 tig0019784:-82828 Msa1376130:CDS
GTATGAATCGAGGTCGTTTC+TGG 0.219744 tig0019784:-83425 Msa1376130:CDS
TGATGTTAGGAGGAAGGTTT+TGG 0.250788 tig0019784:-83647 Msa1376130:CDS
CCATAAGCTTGATCTTCTTC+TGG 0.258919 tig0019784:+82759 None:intergenic
AGAGTTTCTTCTATTGATTT+AGG 0.261536 tig0019784:-83007 Msa1376130:CDS
TGGGGAAGATTCATGGTTGC+TGG 0.279240 tig0019784:-83627 Msa1376130:CDS
TCCCTTTCCTGCTCATTCCT+TGG 0.291476 tig0019784:+83676 None:intergenic
ACCTCTGTCTATTATGTGTT+TGG 0.301401 tig0019784:-83532 Msa1376130:CDS
CCAAAAGTGGAAGATGATTC+TGG 0.302301 tig0019784:-83178 Msa1376130:CDS
TCTTCTGGAGATCAGTGATA+TGG 0.305181 tig0019784:+82774 None:intergenic
CCAGAAGAAGATCAAGCTTA+TGG 0.312383 tig0019784:-82759 Msa1376130:CDS
GATATGGTCAGGGCAACTTT+TGG 0.330665 tig0019784:-83301 Msa1376130:CDS
GGGGAAGATTCATGGTTGCT+GGG 0.377703 tig0019784:-83626 Msa1376130:CDS
TAAGAAATTGGACAGTGTTT+CGG 0.382697 tig0019784:-83575 Msa1376130:CDS
CAGTTGGGTCAAATGATTCC+TGG 0.383826 tig0019784:-83049 Msa1376130:CDS
CAGGTTGCCGGTTTGAAATC+CGG 0.384873 tig0019784:-83751 Msa1376130:CDS
AAAGGGAGAAAGATGATGTT+AGG 0.390427 tig0019784:-83660 Msa1376130:CDS
TGGATAGTCACGTCAAGATT+CGG 0.396746 tig0019784:+83829 None:intergenic
ATGTATGAATTACTTTATCC+TGG 0.397717 tig0019784:+82549 None:intergenic
GTTAGGAACAACAGCTCTTA+AGG 0.401789 tig0019784:+82841 None:intergenic
GAGGTCGTTTCTGGCTAAAT+CGG 0.407099 tig0019784:-83416 Msa1376130:CDS
GCACACTTAAAAGAATTTCC+AGG 0.410186 tig0019784:+83490 None:intergenic
ACTTTAACAATTCCAGAAAC+CGG 0.412032 tig0019784:+82620 None:intergenic
CTTGGCGCAAACCATGCTTA+TGG 0.414623 tig0019784:-83124 Msa1376130:CDS
AAGATTCATGGTTGCTGGGG+TGG 0.418070 tig0019784:-83622 Msa1376130:CDS
TTTGGGCACGAGTTAAGCTT+GGG 0.421749 tig0019784:-83238 Msa1376130:CDS
CTCTTAAGGCGTAAAATCTC+TGG 0.435497 tig0019784:+82855 None:intergenic
GGTTCCTCTCGTCCATTGGC+AGG 0.435606 tig0019784:+82923 None:intergenic
CTTATGGTAATTCTTCCAAC+GGG 0.438633 tig0019784:-83108 Msa1376130:CDS
CAGTGGTTCCTCTCGTCCAT+TGG 0.439512 tig0019784:+82919 None:intergenic
AGGTTGTGTCACATTGTTAA+CGG 0.439902 tig0019784:-83802 Msa1376130:CDS
GAAAGATGATGTTAGGAGGA+AGG 0.440512 tig0019784:-83653 Msa1376130:CDS
TATCTTGCAATGGCAATTGC+TGG 0.440816 tig0019784:+82705 None:intergenic
TTTAATTCTCAGTATGGTCC+TGG 0.444163 tig0019784:-83508 Msa1376130:CDS
GAAGACTCCTCCTCACCTCT+TGG 0.464557 tig0019784:-82980 Msa1376130:CDS
TGAAGAAGACAAGGCTATGT+TGG 0.466385 tig0019784:-83932 Msa1376130:CDS
TATTAGTTTCTCCCCAAAAG+TGG 0.469095 tig0019784:-83191 Msa1376130:CDS
TTCCCCTCCTTGAGTTCTGA+TGG 0.471316 tig0019784:+82523 None:intergenic
TCTATAACCCCGGCTTTAAG+AGG 0.476638 tig0019784:+83367 None:intergenic
ATCTACCTCTGGACAGGCTA+AGG 0.484847 tig0019784:-83275 Msa1376130:CDS
ATACGGTGAGGGACACTGTC+AGG 0.487755 tig0019784:-83713 Msa1376130:CDS
TCGTTTCTGGCTAAATCGGC+TGG 0.495546 tig0019784:-83412 Msa1376130:CDS
GGCCAATGTTTCAACTTCCC+AGG 0.496533 tig0019784:-82567 Msa1376130:CDS
CCAAGTTGGTCTTCTGATGC+TGG 0.500304 tig0019784:-83463 Msa1376130:CDS
CTTCTGGAGATCAGTGATAT+GGG 0.500322 tig0019784:+82775 None:intergenic
GTCCAAGGAATGAGCAGGAA+AGG 0.501601 tig0019784:-83678 Msa1376130:CDS
GGCTATGTTGGAATCTGTGT+TGG 0.505854 tig0019784:-83920 Msa1376130:CDS
AAAGCCGGGGTTATAGAGCT+TGG 0.507196 tig0019784:-83361 Msa1376130:CDS
TGGAGAATCTACCTCTGGAC+AGG 0.507498 tig0019784:-83281 Msa1376130:CDS
TGTTAGAGTTAGCTGCCCCT+TGG 0.517530 tig0019784:-82651 Msa1376130:CDS
TCCAAGGAATGAGCAGGAAA+GGG 0.524680 tig0019784:-83677 Msa1376130:CDS
GACACTGTCAGGATCCCAAA+GGG 0.526579 tig0019784:-83702 Msa1376130:CDS
ATCCTGGGAAGTTGAAACAT+TGG 0.527219 tig0019784:+82565 None:intergenic
CTGCCCCTTGGATATCCACC+CGG 0.528394 tig0019784:-82639 Msa1376130:CDS
TTGACGTGACTATCCACAAA+AGG 0.529097 tig0019784:-83822 Msa1376130:CDS
GGTTATAGAGCTTGGTTCGA+CGG 0.532928 tig0019784:-83353 Msa1376130:CDS
AGAGCTTGGTTCGACGGAGA+TGG 0.536663 tig0019784:-83347 Msa1376130:CDS
CAAAATCGCGTAGTTCCACT+TGG 0.541178 tig0019784:+83777 None:intergenic
AGAGAAAGATGGCTGGTGAG+AGG 0.545343 tig0019784:-83966 None:intergenic
TTAAGTGTGCTAAACCAAGT+TGG 0.545549 tig0019784:-83477 Msa1376130:CDS
GGGAAGATTCATGGTTGCTG+GGG 0.546890 tig0019784:-83625 Msa1376130:CDS
TGTATGAATTACTTTATCCT+GGG 0.549481 tig0019784:+82550 None:intergenic
ATAGCCACCGGATTTCAAAC+CGG 0.550635 tig0019784:+83744 None:intergenic
GCCTGAGAAGTAAAATTCCC+AGG 0.550809 tig0019784:+83031 None:intergenic
GCATCAAATTGTAGCTCAAG+AGG 0.553064 tig0019784:-82588 Msa1376130:CDS
TTGAAGCGGAGAGGCAAAGA+CGG 0.553502 tig0019784:-82889 Msa1376130:CDS
GCTTATGGTAATTCTTCCAA+CGG 0.554315 tig0019784:-83109 Msa1376130:CDS
CCAGCATCAGAAGACCAACT+TGG 0.555302 tig0019784:+83463 None:intergenic
AGTTGGGTCAAATGATTCCT+GGG 0.557673 tig0019784:-83048 Msa1376130:CDS
TGGAAGAATTACCATAAGCA+TGG 0.563019 tig0019784:+83113 None:intergenic
ACATTCTCCATCAGAACTCA+AGG 0.569500 tig0019784:-82530 Msa1376130:CDS
CTTTAACAATTCCAGAAACC+GGG 0.569555 tig0019784:+82621 None:intergenic
GGGAGAAAGATGATGTTAGG+AGG 0.569685 tig0019784:-83657 Msa1376130:CDS
TCCGCTTCAACATGATTCAG+TGG 0.571772 tig0019784:+82902 None:intergenic
TGAAGCGGAGAGGCAAAGAC+GGG 0.572704 tig0019784:-82888 Msa1376130:CDS
GGTGGCTATGTGTTGAAATA+CGG 0.578846 tig0019784:-83730 Msa1376130:CDS
AGAAACCGGGTGGATATCCA+AGG 0.585451 tig0019784:+82634 None:intergenic
TGGGCACGAGTTAAGCTTGG+GGG 0.592030 tig0019784:-83236 Msa1376130:CDS
TGAAAATCAATATCTTGCAA+TGG 0.592942 tig0019784:+82695 None:intergenic
CTCCATCAGAACTCAAGGAG+GGG 0.593410 tig0019784:-82525 Msa1376130:CDS
GTATAGTAGAGAAAGATGGC+TGG 0.593720 tig0019784:-83973 None:intergenic
TAACATTTCTAAAATGGACA+AGG 0.599447 tig0019784:-82822 Msa1376130:CDS
CTCGTCTCCAAGAGGTGAGG+AGG 0.602197 tig0019784:+82973 None:intergenic
AGAGGGAGAAAACCTGCCAA+TGG 0.605353 tig0019784:-82935 Msa1376130:CDS
ACCACTGAATCATGTTGAAG+CGG 0.606334 tig0019784:-82903 Msa1376130:CDS
GTATTTCAACACATAGCCAC+CGG 0.614051 tig0019784:+83732 None:intergenic
GCTATGTTGGAATCTGTGTT+GGG 0.620643 tig0019784:-83919 Msa1376130:CDS
GGACACTGTCAGGATCCCAA+AGG 0.622420 tig0019784:-83703 Msa1376130:CDS
TCTCCATCAGAACTCAAGGA+GGG 0.623115 tig0019784:-82526 Msa1376130:CDS
CACTGTCAGGATCCCAAAGG+GGG 0.625514 tig0019784:-83700 Msa1376130:CDS
TTGTAAGAATGAAGAAGACA+AGG 0.629373 tig0019784:-83941 Msa1376130:CDS
GAATCATTTGACCCAACTGA+GGG 0.642194 tig0019784:+83053 None:intergenic
CCGCTCGTCTCCAAGAGGTG+AGG 0.645733 tig0019784:+82970 None:intergenic
CCTCACCTCTTGGAGACGAG+CGG 0.646158 tig0019784:-82970 Msa1376130:CDS
TATGGTAATTCTTCCAACGG+GGG 0.650209 tig0019784:-83106 Msa1376130:CDS
TTCTCCATCAGAACTCAAGG+AGG 0.654759 tig0019784:-82527 Msa1376130:CDS
AAAACCTGCCAATGGACGAG+AGG 0.654921 tig0019784:-82927 Msa1376130:CDS
GAAACCGGGTGGATATCCAA+GGG 0.657964 tig0019784:+82635 None:intergenic
TGAATCATGTTGAAGCGGAG+AGG 0.659736 tig0019784:-82898 Msa1376130:CDS
GGAATCATTTGACCCAACTG+AGG 0.660288 tig0019784:+83052 None:intergenic
GGCTTCCGCTCGTCTCCAAG+AGG 0.660984 tig0019784:+82965 None:intergenic
GTTGCCGGTTTGAAATCCGG+TGG 0.661652 tig0019784:-83748 Msa1376130:CDS
ACATTGTTAACGGTTCCAAG+TGG 0.664341 tig0019784:-83792 Msa1376130:CDS
TTGGGCACGAGTTAAGCTTG+GGG 0.666411 tig0019784:-83237 Msa1376130:CDS
CGAACCAAGCTCTATAACCC+CGG 0.673641 tig0019784:+83357 None:intergenic
GTTTAAATCAGTATGAATCG+AGG 0.676935 tig0019784:-83435 Msa1376130:CDS
GAGATGGTGCCTGAAGAGCA+AGG 0.686748 tig0019784:-83331 Msa1376130:CDS
ACACTGTCAGGATCCCAAAG+GGG 0.690461 tig0019784:-83701 Msa1376130:CDS
TAACAATTCCAGAAACCGGG+TGG 0.692180 tig0019784:+82624 None:intergenic
ACCAAACACATAATAGACAG+AGG 0.698940 tig0019784:+83531 None:intergenic
ATTCGGCGGAACAATAACAT+CGG 0.702866 tig0019784:+83846 None:intergenic
TATGTGTTGAAATACGGTGA+GGG 0.713229 tig0019784:-83724 Msa1376130:CDS
TTATGGTAATTCTTCCAACG+GGG 0.725236 tig0019784:-83107 Msa1376130:CDS
ATAGTCACGTCAAGATTCGG+CGG 0.725406 tig0019784:+83832 None:intergenic
AAACCGGGTGGATATCCAAG+GGG 0.728834 tig0019784:+82636 None:intergenic
CTATGTGTTGAAATACGGTG+AGG 0.742411 tig0019784:-83725 Msa1376130:CDS
GGGTGTAGGAGACAGCAACG+AGG 0.803375 tig0019784:-83086 Msa1376130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAGAATATTTATAAGAAAT+TGG - tig0019784:82840-82859 Msa1376130:CDS 15.0%
!! TGAGAATATTTATAAGAAAT+TGG - tig0019784:82840-82859 Msa1376130:CDS 15.0%
!!! TTTGGTTTTAATTCTCAGTA+TGG - tig0019784:82913-82932 Msa1376130:CDS 25.0%
!!! AGAGTTTCTTCTATTGATTT+AGG - tig0019784:83420-83439 Msa1376130:CDS 25.0%
!! TGTTCCTAACATTTCTAAAA+TGG - tig0019784:83599-83618 Msa1376130:CDS 25.0%
!!! TTGTCCATTTTAGAAATGTT+AGG + tig0019784:83606-83625 None:intergenic 25.0%
!!! TAACATTTCTAAAATGGACA+AGG - tig0019784:83605-83624 Msa1376130:CDS 25.0%
! TATGGAAACTGAAAAAAACA+TGG - tig0019784:83686-83705 Msa1376130:CDS 25.0%
! TGAAAATCAATATCTTGCAA+TGG + tig0019784:83735-83754 None:intergenic 25.0%
!!! ATTGCAAGATATTGATTTTC+AGG - tig0019784:83734-83753 Msa1376130:CDS 25.0%
! TGTATGAATTACTTTATCCT+GGG + tig0019784:83880-83899 None:intergenic 25.0%
! ATGTATGAATTACTTTATCC+TGG + tig0019784:83881-83900 None:intergenic 25.0%
!!! TTTGGTTTTAATTCTCAGTA+TGG - tig0019784:82913-82932 Msa1376130:CDS 25.0%
!!! AGAGTTTCTTCTATTGATTT+AGG - tig0019784:83420-83439 Msa1376130:CDS 25.0%
!! TGTTCCTAACATTTCTAAAA+TGG - tig0019784:83599-83618 Msa1376130:CDS 25.0%
!!! TTGTCCATTTTAGAAATGTT+AGG + tig0019784:83606-83625 None:intergenic 25.0%
!!! TAACATTTCTAAAATGGACA+AGG - tig0019784:83605-83624 Msa1376130:CDS 25.0%
! TATGGAAACTGAAAAAAACA+TGG - tig0019784:83686-83705 Msa1376130:CDS 25.0%
! TGAAAATCAATATCTTGCAA+TGG + tig0019784:83735-83754 None:intergenic 25.0%
!!! ATTGCAAGATATTGATTTTC+AGG - tig0019784:83734-83753 Msa1376130:CDS 25.0%
! TGTATGAATTACTTTATCCT+GGG + tig0019784:83880-83899 None:intergenic 25.0%
! ATGTATGAATTACTTTATCC+TGG + tig0019784:83881-83900 None:intergenic 25.0%
TTGTAAGAATGAAGAAGACA+AGG - tig0019784:82486-82505 Msa1376130:CDS 30.0%
TAAGAAATTGGACAGTGTTT+CGG - tig0019784:82852-82871 Msa1376130:CDS 30.0%
GTTTAAATCAGTATGAATCG+AGG - tig0019784:82992-83011 Msa1376130:CDS 30.0%
AAAAAACATGGCTGACAATA+AGG - tig0019784:83698-83717 Msa1376130:CDS 30.0%
AAAAACATGGCTGACAATAA+GGG - tig0019784:83699-83718 Msa1376130:CDS 30.0%
ACTTTAACAATTCCAGAAAC+CGG + tig0019784:83810-83829 None:intergenic 30.0%
TTGTAAGAATGAAGAAGACA+AGG - tig0019784:82486-82505 Msa1376130:CDS 30.0%
TAAGAAATTGGACAGTGTTT+CGG - tig0019784:82852-82871 Msa1376130:CDS 30.0%
GTTTAAATCAGTATGAATCG+AGG - tig0019784:82992-83011 Msa1376130:CDS 30.0%
AAAAAACATGGCTGACAATA+AGG - tig0019784:83698-83717 Msa1376130:CDS 30.0%
AAAAACATGGCTGACAATAA+GGG - tig0019784:83699-83718 Msa1376130:CDS 30.0%
ACTTTAACAATTCCAGAAAC+CGG + tig0019784:83810-83829 None:intergenic 30.0%
AGGTTGTGTCACATTGTTAA+CGG - tig0019784:82625-82644 Msa1376130:CDS 35.0%
TATGTGTTGAAATACGGTGA+GGG - tig0019784:82703-82722 Msa1376130:CDS 35.0%
AAAGGGAGAAAGATGATGTT+AGG - tig0019784:82767-82786 Msa1376130:CDS 35.0%
ACCTCTGTCTATTATGTGTT+TGG - tig0019784:82895-82914 Msa1376130:CDS 35.0%
ACCAAACACATAATAGACAG+AGG + tig0019784:82899-82918 None:intergenic 35.0%
TTTAATTCTCAGTATGGTCC+TGG - tig0019784:82919-82938 Msa1376130:CDS 35.0%
GCACACTTAAAAGAATTTCC+AGG + tig0019784:82940-82959 None:intergenic 35.0%
TTAAGTGTGCTAAACCAAGT+TGG - tig0019784:82950-82969 Msa1376130:CDS 35.0%
!!! AGAGCAAGGTTTTTTGGATA+TGG - tig0019784:83110-83129 Msa1376130:CDS 35.0%
!!! AAGGTTTTTTGGATATGGTC+AGG - tig0019784:83115-83134 Msa1376130:CDS 35.0%
!!! AGGTTTTTTGGATATGGTCA+GGG - tig0019784:83116-83135 Msa1376130:CDS 35.0%
! AAGGTAGCACCAAAGATTTT+TGG - tig0019784:83171-83190 Msa1376130:CDS 35.0%
! AGGTAGCACCAAAGATTTTT+GGG - tig0019784:83172-83191 Msa1376130:CDS 35.0%
! TATTAGTTTCTCCCCAAAAG+TGG - tig0019784:83236-83255 Msa1376130:CDS 35.0%
!! AGAATCATCTTCCACTTTTG+GGG + tig0019784:83250-83269 None:intergenic 35.0%
!! CAGAATCATCTTCCACTTTT+GGG + tig0019784:83251-83270 None:intergenic 35.0%
TGGAAGAATTACCATAAGCA+TGG + tig0019784:83317-83336 None:intergenic 35.0%
GCTTATGGTAATTCTTCCAA+CGG - tig0019784:83318-83337 Msa1376130:CDS 35.0%
CTTATGGTAATTCTTCCAAC+GGG - tig0019784:83319-83338 Msa1376130:CDS 35.0%
TTATGGTAATTCTTCCAACG+GGG - tig0019784:83320-83339 Msa1376130:CDS 35.0%
CTTTAACAATTCCAGAAACC+GGG + tig0019784:83809-83828 None:intergenic 35.0%
AGGTTGTGTCACATTGTTAA+CGG - tig0019784:82625-82644 Msa1376130:CDS 35.0%
TATGTGTTGAAATACGGTGA+GGG - tig0019784:82703-82722 Msa1376130:CDS 35.0%
AAAGGGAGAAAGATGATGTT+AGG - tig0019784:82767-82786 Msa1376130:CDS 35.0%
ACCTCTGTCTATTATGTGTT+TGG - tig0019784:82895-82914 Msa1376130:CDS 35.0%
ACCAAACACATAATAGACAG+AGG + tig0019784:82899-82918 None:intergenic 35.0%
TTTAATTCTCAGTATGGTCC+TGG - tig0019784:82919-82938 Msa1376130:CDS 35.0%
GCACACTTAAAAGAATTTCC+AGG + tig0019784:82940-82959 None:intergenic 35.0%
TTAAGTGTGCTAAACCAAGT+TGG - tig0019784:82950-82969 Msa1376130:CDS 35.0%
!!! AGAGCAAGGTTTTTTGGATA+TGG - tig0019784:83110-83129 Msa1376130:CDS 35.0%
!!! AAGGTTTTTTGGATATGGTC+AGG - tig0019784:83115-83134 Msa1376130:CDS 35.0%
!!! AGGTTTTTTGGATATGGTCA+GGG - tig0019784:83116-83135 Msa1376130:CDS 35.0%
! AAGGTAGCACCAAAGATTTT+TGG - tig0019784:83171-83190 Msa1376130:CDS 35.0%
! AGGTAGCACCAAAGATTTTT+GGG - tig0019784:83172-83191 Msa1376130:CDS 35.0%
! TATTAGTTTCTCCCCAAAAG+TGG - tig0019784:83236-83255 Msa1376130:CDS 35.0%
!! AGAATCATCTTCCACTTTTG+GGG + tig0019784:83250-83269 None:intergenic 35.0%
!! CAGAATCATCTTCCACTTTT+GGG + tig0019784:83251-83270 None:intergenic 35.0%
TGGAAGAATTACCATAAGCA+TGG + tig0019784:83317-83336 None:intergenic 35.0%
GCTTATGGTAATTCTTCCAA+CGG - tig0019784:83318-83337 Msa1376130:CDS 35.0%
CTTATGGTAATTCTTCCAAC+GGG - tig0019784:83319-83338 Msa1376130:CDS 35.0%
TTATGGTAATTCTTCCAACG+GGG - tig0019784:83320-83339 Msa1376130:CDS 35.0%
CTTTAACAATTCCAGAAACC+GGG + tig0019784:83809-83828 None:intergenic 35.0%
TGAAGAAGACAAGGCTATGT+TGG - tig0019784:82495-82514 Msa1376130:CDS 40.0%
GCTATGTTGGAATCTGTGTT+GGG - tig0019784:82508-82527 Msa1376130:CDS 40.0%
ATTCGGCGGAACAATAACAT+CGG + tig0019784:82584-82603 None:intergenic 40.0%
TGGATAGTCACGTCAAGATT+CGG + tig0019784:82601-82620 None:intergenic 40.0%
TTGACGTGACTATCCACAAA+AGG - tig0019784:82605-82624 Msa1376130:CDS 40.0%
! CAATGTGACACAACCTTTTG+TGG + tig0019784:82621-82640 None:intergenic 40.0%
ACATTGTTAACGGTTCCAAG+TGG - tig0019784:82635-82654 Msa1376130:CDS 40.0%
GTATTTCAACACATAGCCAC+CGG + tig0019784:82698-82717 None:intergenic 40.0%
GGTGGCTATGTGTTGAAATA+CGG - tig0019784:82697-82716 Msa1376130:CDS 40.0%
CTATGTGTTGAAATACGGTG+AGG - tig0019784:82702-82721 Msa1376130:CDS 40.0%
GAAAGATGATGTTAGGAGGA+AGG - tig0019784:82774-82793 Msa1376130:CDS 40.0%
! TGATGTTAGGAGGAAGGTTT+TGG - tig0019784:82780-82799 Msa1376130:CDS 40.0%
! GATGTTAGGAGGAAGGTTTT+GGG - tig0019784:82781-82800 Msa1376130:CDS 40.0%
! ATGTTAGGAGGAAGGTTTTG+GGG - tig0019784:82782-82801 Msa1376130:CDS 40.0%
!!! AAGGTTTTGGGGAAGATTCA+TGG - tig0019784:82793-82812 Msa1376130:CDS 40.0%
! GCTTTTGTACCTCTTAAAGC+CGG - tig0019784:83051-83070 Msa1376130:CDS 40.0%
! CTTTTGTACCTCTTAAAGCC+GGG - tig0019784:83052-83071 Msa1376130:CDS 40.0%
! TTTTGTACCTCTTAAAGCCG+GGG - tig0019784:83053-83072 Msa1376130:CDS 40.0%
TCCAAAAAACCTTGCTCTTC+AGG + tig0019784:83108-83127 None:intergenic 40.0%
!! ACTTTTGGAGAATCTACCTC+TGG - tig0019784:83141-83160 Msa1376130:CDS 40.0%
AACTCGTGCCCAAAAATCTT+TGG + tig0019784:83183-83202 None:intergenic 40.0%
! CCAGAATCATCTTCCACTTT+TGG + tig0019784:83252-83271 None:intergenic 40.0%
CCAAAAGTGGAAGATGATTC+TGG - tig0019784:83249-83268 Msa1376130:CDS 40.0%
TATGGTAATTCTTCCAACGG+GGG - tig0019784:83321-83340 Msa1376130:CDS 40.0%
!! GCAAAAAAGTACCCTCAGTT+GGG - tig0019784:83363-83382 Msa1376130:CDS 40.0%
GAATCATTTGACCCAACTGA+GGG + tig0019784:83377-83396 None:intergenic 40.0%
AGTTGGGTCAAATGATTCCT+GGG - tig0019784:83379-83398 Msa1376130:CDS 40.0%
! TCCTGGGAATTTTACTTCTC+AGG - tig0019784:83395-83414 Msa1376130:CDS 40.0%
! GGTTTTCTCCCTCTTTTTCT+AGG + tig0019784:83486-83505 None:intergenic 40.0%
ACCACTGAATCATGTTGAAG+CGG - tig0019784:83524-83543 Msa1376130:CDS 40.0%
CTCTTAAGGCGTAAAATCTC+TGG + tig0019784:83575-83594 None:intergenic 40.0%
GTTAGGAACAACAGCTCTTA+AGG + tig0019784:83589-83608 None:intergenic 40.0%
! CTTCTGGAGATCAGTGATAT+GGG + tig0019784:83655-83674 None:intergenic 40.0%
! TCTTCTGGAGATCAGTGATA+TGG + tig0019784:83656-83675 None:intergenic 40.0%
CCATAAGCTTGATCTTCTTC+TGG + tig0019784:83671-83690 None:intergenic 40.0%
CCAGAAGAAGATCAAGCTTA+TGG - tig0019784:83668-83687 Msa1376130:CDS 40.0%
TATCTTGCAATGGCAATTGC+TGG + tig0019784:83725-83744 None:intergenic 40.0%
!!! TATTGATTTTCAGGCTCGAC+AGG - tig0019784:83743-83762 Msa1376130:CDS 40.0%
GCATCAAATTGTAGCTCAAG+AGG - tig0019784:83839-83858 Msa1376130:CDS 40.0%
ATCCTGGGAAGTTGAAACAT+TGG + tig0019784:83865-83884 None:intergenic 40.0%
ACATTCTCCATCAGAACTCA+AGG - tig0019784:83897-83916 Msa1376130:CDS 40.0%
TGAAGAAGACAAGGCTATGT+TGG - tig0019784:82495-82514 Msa1376130:CDS 40.0%
GCTATGTTGGAATCTGTGTT+GGG - tig0019784:82508-82527 Msa1376130:CDS 40.0%
ATTCGGCGGAACAATAACAT+CGG + tig0019784:82584-82603 None:intergenic 40.0%
TGGATAGTCACGTCAAGATT+CGG + tig0019784:82601-82620 None:intergenic 40.0%
TTGACGTGACTATCCACAAA+AGG - tig0019784:82605-82624 Msa1376130:CDS 40.0%
! CAATGTGACACAACCTTTTG+TGG + tig0019784:82621-82640 None:intergenic 40.0%
ACATTGTTAACGGTTCCAAG+TGG - tig0019784:82635-82654 Msa1376130:CDS 40.0%
GTATTTCAACACATAGCCAC+CGG + tig0019784:82698-82717 None:intergenic 40.0%
GGTGGCTATGTGTTGAAATA+CGG - tig0019784:82697-82716 Msa1376130:CDS 40.0%
CTATGTGTTGAAATACGGTG+AGG - tig0019784:82702-82721 Msa1376130:CDS 40.0%
GAAAGATGATGTTAGGAGGA+AGG - tig0019784:82774-82793 Msa1376130:CDS 40.0%
! TGATGTTAGGAGGAAGGTTT+TGG - tig0019784:82780-82799 Msa1376130:CDS 40.0%
! GATGTTAGGAGGAAGGTTTT+GGG - tig0019784:82781-82800 Msa1376130:CDS 40.0%
! ATGTTAGGAGGAAGGTTTTG+GGG - tig0019784:82782-82801 Msa1376130:CDS 40.0%
!!! AAGGTTTTGGGGAAGATTCA+TGG - tig0019784:82793-82812 Msa1376130:CDS 40.0%
! GCTTTTGTACCTCTTAAAGC+CGG - tig0019784:83051-83070 Msa1376130:CDS 40.0%
! CTTTTGTACCTCTTAAAGCC+GGG - tig0019784:83052-83071 Msa1376130:CDS 40.0%
! TTTTGTACCTCTTAAAGCCG+GGG - tig0019784:83053-83072 Msa1376130:CDS 40.0%
TCCAAAAAACCTTGCTCTTC+AGG + tig0019784:83108-83127 None:intergenic 40.0%
!! ACTTTTGGAGAATCTACCTC+TGG - tig0019784:83141-83160 Msa1376130:CDS 40.0%
AACTCGTGCCCAAAAATCTT+TGG + tig0019784:83183-83202 None:intergenic 40.0%
! CCAGAATCATCTTCCACTTT+TGG + tig0019784:83252-83271 None:intergenic 40.0%
CCAAAAGTGGAAGATGATTC+TGG - tig0019784:83249-83268 Msa1376130:CDS 40.0%
TATGGTAATTCTTCCAACGG+GGG - tig0019784:83321-83340 Msa1376130:CDS 40.0%
!! GCAAAAAAGTACCCTCAGTT+GGG - tig0019784:83363-83382 Msa1376130:CDS 40.0%
GAATCATTTGACCCAACTGA+GGG + tig0019784:83377-83396 None:intergenic 40.0%
AGTTGGGTCAAATGATTCCT+GGG - tig0019784:83379-83398 Msa1376130:CDS 40.0%
! TCCTGGGAATTTTACTTCTC+AGG - tig0019784:83395-83414 Msa1376130:CDS 40.0%
! GGTTTTCTCCCTCTTTTTCT+AGG + tig0019784:83486-83505 None:intergenic 40.0%
ACCACTGAATCATGTTGAAG+CGG - tig0019784:83524-83543 Msa1376130:CDS 40.0%
CTCTTAAGGCGTAAAATCTC+TGG + tig0019784:83575-83594 None:intergenic 40.0%
GTTAGGAACAACAGCTCTTA+AGG + tig0019784:83589-83608 None:intergenic 40.0%
! CTTCTGGAGATCAGTGATAT+GGG + tig0019784:83655-83674 None:intergenic 40.0%
! TCTTCTGGAGATCAGTGATA+TGG + tig0019784:83656-83675 None:intergenic 40.0%
CCATAAGCTTGATCTTCTTC+TGG + tig0019784:83671-83690 None:intergenic 40.0%
CCAGAAGAAGATCAAGCTTA+TGG - tig0019784:83668-83687 Msa1376130:CDS 40.0%
TATCTTGCAATGGCAATTGC+TGG + tig0019784:83725-83744 None:intergenic 40.0%
!!! TATTGATTTTCAGGCTCGAC+AGG - tig0019784:83743-83762 Msa1376130:CDS 40.0%
GCATCAAATTGTAGCTCAAG+AGG - tig0019784:83839-83858 Msa1376130:CDS 40.0%
ATCCTGGGAAGTTGAAACAT+TGG + tig0019784:83865-83884 None:intergenic 40.0%
ACATTCTCCATCAGAACTCA+AGG - tig0019784:83897-83916 Msa1376130:CDS 40.0%
GGCTATGTTGGAATCTGTGT+TGG - tig0019784:82507-82526 Msa1376130:CDS 45.0%
ATAGTCACGTCAAGATTCGG+CGG + tig0019784:82598-82617 None:intergenic 45.0%
CAAAATCGCGTAGTTCCACT+TGG + tig0019784:82653-82672 None:intergenic 45.0%
! AGTGGAACTACGCGATTTTG+TGG - tig0019784:82653-82672 Msa1376130:CDS 45.0%
ATAGCCACCGGATTTCAAAC+CGG + tig0019784:82686-82705 None:intergenic 45.0%
TCCAAGGAATGAGCAGGAAA+GGG - tig0019784:82750-82769 Msa1376130:CDS 45.0%
GGGAGAAAGATGATGTTAGG+AGG - tig0019784:82770-82789 Msa1376130:CDS 45.0%
GTATGAATCGAGGTCGTTTC+TGG - tig0019784:83002-83021 Msa1376130:CDS 45.0%
!! GAGGTCGTTTCTGGCTAAAT+CGG - tig0019784:83011-83030 Msa1376130:CDS 45.0%
!! TCTATAACCCCGGCTTTAAG+AGG + tig0019784:83063-83082 None:intergenic 45.0%
! GGTTATAGAGCTTGGTTCGA+CGG - tig0019784:83074-83093 Msa1376130:CDS 45.0%
!!! GCCTGAAGAGCAAGGTTTTT+TGG - tig0019784:83104-83123 Msa1376130:CDS 45.0%
! GATATGGTCAGGGCAACTTT+TGG - tig0019784:83126-83145 Msa1376130:CDS 45.0%
! TTTTGGGCACGAGTTAAGCT+TGG - tig0019784:83188-83207 Msa1376130:CDS 45.0%
TTTGGGCACGAGTTAAGCTT+GGG - tig0019784:83189-83208 Msa1376130:CDS 45.0%
TCTTACGAAGTACAAGCGCT+TGG - tig0019784:83285-83304 Msa1376130:CDS 45.0%
!! GGCAAAAAAGTACCCTCAGT+TGG - tig0019784:83362-83381 Msa1376130:CDS 45.0%
GGAATCATTTGACCCAACTG+AGG + tig0019784:83378-83397 None:intergenic 45.0%
CAGTTGGGTCAAATGATTCC+TGG - tig0019784:83378-83397 Msa1376130:CDS 45.0%
GCCTGAGAAGTAAAATTCCC+AGG + tig0019784:83399-83418 None:intergenic 45.0%
AGCGGAAGCCTAGAAAAAGA+GGG - tig0019784:83475-83494 Msa1376130:CDS 45.0%
TCCGCTTCAACATGATTCAG+TGG + tig0019784:83528-83547 None:intergenic 45.0%
TGAATCATGTTGAAGCGGAG+AGG - tig0019784:83529-83548 Msa1376130:CDS 45.0%
ATGGACAAGGCTTCTCTTCT+TGG - tig0019784:83618-83637 Msa1376130:CDS 45.0%
TAACAATTCCAGAAACCGGG+TGG + tig0019784:83806-83825 None:intergenic 45.0%
TTCTCCATCAGAACTCAAGG+AGG - tig0019784:83900-83919 Msa1376130:CDS 45.0%
TCTCCATCAGAACTCAAGGA+GGG - tig0019784:83901-83920 Msa1376130:CDS 45.0%
GGCTATGTTGGAATCTGTGT+TGG - tig0019784:82507-82526 Msa1376130:CDS 45.0%
ATAGTCACGTCAAGATTCGG+CGG + tig0019784:82598-82617 None:intergenic 45.0%
CAAAATCGCGTAGTTCCACT+TGG + tig0019784:82653-82672 None:intergenic 45.0%
! AGTGGAACTACGCGATTTTG+TGG - tig0019784:82653-82672 Msa1376130:CDS 45.0%
ATAGCCACCGGATTTCAAAC+CGG + tig0019784:82686-82705 None:intergenic 45.0%
TCCAAGGAATGAGCAGGAAA+GGG - tig0019784:82750-82769 Msa1376130:CDS 45.0%
GGGAGAAAGATGATGTTAGG+AGG - tig0019784:82770-82789 Msa1376130:CDS 45.0%
GTATGAATCGAGGTCGTTTC+TGG - tig0019784:83002-83021 Msa1376130:CDS 45.0%
!! GAGGTCGTTTCTGGCTAAAT+CGG - tig0019784:83011-83030 Msa1376130:CDS 45.0%
!! TCTATAACCCCGGCTTTAAG+AGG + tig0019784:83063-83082 None:intergenic 45.0%
! GGTTATAGAGCTTGGTTCGA+CGG - tig0019784:83074-83093 Msa1376130:CDS 45.0%
!!! GCCTGAAGAGCAAGGTTTTT+TGG - tig0019784:83104-83123 Msa1376130:CDS 45.0%
! GATATGGTCAGGGCAACTTT+TGG - tig0019784:83126-83145 Msa1376130:CDS 45.0%
! TTTTGGGCACGAGTTAAGCT+TGG - tig0019784:83188-83207 Msa1376130:CDS 45.0%
TTTGGGCACGAGTTAAGCTT+GGG - tig0019784:83189-83208 Msa1376130:CDS 45.0%
TCTTACGAAGTACAAGCGCT+TGG - tig0019784:83285-83304 Msa1376130:CDS 45.0%
!! GGCAAAAAAGTACCCTCAGT+TGG - tig0019784:83362-83381 Msa1376130:CDS 45.0%
GGAATCATTTGACCCAACTG+AGG + tig0019784:83378-83397 None:intergenic 45.0%
CAGTTGGGTCAAATGATTCC+TGG - tig0019784:83378-83397 Msa1376130:CDS 45.0%
GCCTGAGAAGTAAAATTCCC+AGG + tig0019784:83399-83418 None:intergenic 45.0%
AGCGGAAGCCTAGAAAAAGA+GGG - tig0019784:83475-83494 Msa1376130:CDS 45.0%
TCCGCTTCAACATGATTCAG+TGG + tig0019784:83528-83547 None:intergenic 45.0%
TGAATCATGTTGAAGCGGAG+AGG - tig0019784:83529-83548 Msa1376130:CDS 45.0%
ATGGACAAGGCTTCTCTTCT+TGG - tig0019784:83618-83637 Msa1376130:CDS 45.0%
TAACAATTCCAGAAACCGGG+TGG + tig0019784:83806-83825 None:intergenic 45.0%
TTCTCCATCAGAACTCAAGG+AGG - tig0019784:83900-83919 Msa1376130:CDS 45.0%
TCTCCATCAGAACTCAAGGA+GGG - tig0019784:83901-83920 Msa1376130:CDS 45.0%
! GAACTACGCGATTTTGTGGC+AGG - tig0019784:82657-82676 Msa1376130:CDS 50.0%
!! CAGGTTGCCGGTTTGAAATC+CGG - tig0019784:82676-82695 Msa1376130:CDS 50.0%
GACACTGTCAGGATCCCAAA+GGG - tig0019784:82725-82744 Msa1376130:CDS 50.0%
ACACTGTCAGGATCCCAAAG+GGG - tig0019784:82726-82745 Msa1376130:CDS 50.0%
TCATTCCTTGGACCCCCTTT+GGG + tig0019784:82742-82761 None:intergenic 50.0%
GTCCAAGGAATGAGCAGGAA+AGG - tig0019784:82749-82768 Msa1376130:CDS 50.0%
TCCCTTTCCTGCTCATTCCT+TGG + tig0019784:82754-82773 None:intergenic 50.0%
TGGGGAAGATTCATGGTTGC+TGG - tig0019784:82800-82819 Msa1376130:CDS 50.0%
GGGGAAGATTCATGGTTGCT+GGG - tig0019784:82801-82820 Msa1376130:CDS 50.0%
GGGAAGATTCATGGTTGCTG+GGG - tig0019784:82802-82821 Msa1376130:CDS 50.0%
AAGATTCATGGTTGCTGGGG+TGG - tig0019784:82805-82824 Msa1376130:CDS 50.0%
CCAGCATCAGAAGACCAACT+TGG + tig0019784:82967-82986 None:intergenic 50.0%
!! CCAAGTTGGTCTTCTGATGC+TGG - tig0019784:82964-82983 Msa1376130:CDS 50.0%
!! TCGTTTCTGGCTAAATCGGC+TGG - tig0019784:83015-83034 Msa1376130:CDS 50.0%
AAAGCCGGGGTTATAGAGCT+TGG - tig0019784:83066-83085 Msa1376130:CDS 50.0%
CGAACCAAGCTCTATAACCC+CGG + tig0019784:83073-83092 None:intergenic 50.0%
TGGAGAATCTACCTCTGGAC+AGG - tig0019784:83146-83165 Msa1376130:CDS 50.0%
ATCTACCTCTGGACAGGCTA+AGG - tig0019784:83152-83171 Msa1376130:CDS 50.0%
TTGGGCACGAGTTAAGCTTG+GGG - tig0019784:83190-83209 Msa1376130:CDS 50.0%
CTTGGCGCAAACCATGCTTA+TGG - tig0019784:83303-83322 Msa1376130:CDS 50.0%
! AATTCTTCCAACGGGGGTGT+AGG - tig0019784:83327-83346 Msa1376130:CDS 50.0%
GAGCGGAAGCCTAGAAAAAG+AGG - tig0019784:83474-83493 Msa1376130:CDS 50.0%
AGAGGGAGAAAACCTGCCAA+TGG - tig0019784:83492-83511 Msa1376130:CDS 50.0%
AAAACCTGCCAATGGACGAG+AGG - tig0019784:83500-83519 Msa1376130:CDS 50.0%
TTGAAGCGGAGAGGCAAAGA+CGG - tig0019784:83538-83557 Msa1376130:CDS 50.0%
TGTTAGAGTTAGCTGCCCCT+TGG - tig0019784:83776-83795 Msa1376130:CDS 50.0%
AAACCGGGTGGATATCCAAG+GGG + tig0019784:83794-83813 None:intergenic 50.0%
GAAACCGGGTGGATATCCAA+GGG + tig0019784:83795-83814 None:intergenic 50.0%
AGAAACCGGGTGGATATCCA+AGG + tig0019784:83796-83815 None:intergenic 50.0%
TTGGATATCCACCCGGTTTC+TGG - tig0019784:83795-83814 Msa1376130:CDS 50.0%
GGCCAATGTTTCAACTTCCC+AGG - tig0019784:83860-83879 Msa1376130:CDS 50.0%
CTCCATCAGAACTCAAGGAG+GGG - tig0019784:83902-83921 Msa1376130:CDS 50.0%
! TTCCCCTCCTTGAGTTCTGA+TGG + tig0019784:83907-83926 None:intergenic 50.0%
! GAACTACGCGATTTTGTGGC+AGG - tig0019784:82657-82676 Msa1376130:CDS 50.0%
!! CAGGTTGCCGGTTTGAAATC+CGG - tig0019784:82676-82695 Msa1376130:CDS 50.0%
GACACTGTCAGGATCCCAAA+GGG - tig0019784:82725-82744 Msa1376130:CDS 50.0%
ACACTGTCAGGATCCCAAAG+GGG - tig0019784:82726-82745 Msa1376130:CDS 50.0%
TCATTCCTTGGACCCCCTTT+GGG + tig0019784:82742-82761 None:intergenic 50.0%
GTCCAAGGAATGAGCAGGAA+AGG - tig0019784:82749-82768 Msa1376130:CDS 50.0%
TCCCTTTCCTGCTCATTCCT+TGG + tig0019784:82754-82773 None:intergenic 50.0%
TGGGGAAGATTCATGGTTGC+TGG - tig0019784:82800-82819 Msa1376130:CDS 50.0%
GGGGAAGATTCATGGTTGCT+GGG - tig0019784:82801-82820 Msa1376130:CDS 50.0%
GGGAAGATTCATGGTTGCTG+GGG - tig0019784:82802-82821 Msa1376130:CDS 50.0%
AAGATTCATGGTTGCTGGGG+TGG - tig0019784:82805-82824 Msa1376130:CDS 50.0%
CCAGCATCAGAAGACCAACT+TGG + tig0019784:82967-82986 None:intergenic 50.0%
!! CCAAGTTGGTCTTCTGATGC+TGG - tig0019784:82964-82983 Msa1376130:CDS 50.0%
!! TCGTTTCTGGCTAAATCGGC+TGG - tig0019784:83015-83034 Msa1376130:CDS 50.0%
AAAGCCGGGGTTATAGAGCT+TGG - tig0019784:83066-83085 Msa1376130:CDS 50.0%
CGAACCAAGCTCTATAACCC+CGG + tig0019784:83073-83092 None:intergenic 50.0%
TGGAGAATCTACCTCTGGAC+AGG - tig0019784:83146-83165 Msa1376130:CDS 50.0%
ATCTACCTCTGGACAGGCTA+AGG - tig0019784:83152-83171 Msa1376130:CDS 50.0%
TTGGGCACGAGTTAAGCTTG+GGG - tig0019784:83190-83209 Msa1376130:CDS 50.0%
CTTGGCGCAAACCATGCTTA+TGG - tig0019784:83303-83322 Msa1376130:CDS 50.0%
! AATTCTTCCAACGGGGGTGT+AGG - tig0019784:83327-83346 Msa1376130:CDS 50.0%
GAGCGGAAGCCTAGAAAAAG+AGG - tig0019784:83474-83493 Msa1376130:CDS 50.0%
AGAGGGAGAAAACCTGCCAA+TGG - tig0019784:83492-83511 Msa1376130:CDS 50.0%
AAAACCTGCCAATGGACGAG+AGG - tig0019784:83500-83519 Msa1376130:CDS 50.0%
TTGAAGCGGAGAGGCAAAGA+CGG - tig0019784:83538-83557 Msa1376130:CDS 50.0%
TGTTAGAGTTAGCTGCCCCT+TGG - tig0019784:83776-83795 Msa1376130:CDS 50.0%
AAACCGGGTGGATATCCAAG+GGG + tig0019784:83794-83813 None:intergenic 50.0%
GAAACCGGGTGGATATCCAA+GGG + tig0019784:83795-83814 None:intergenic 50.0%
AGAAACCGGGTGGATATCCA+AGG + tig0019784:83796-83815 None:intergenic 50.0%
TTGGATATCCACCCGGTTTC+TGG - tig0019784:83795-83814 Msa1376130:CDS 50.0%
GGCCAATGTTTCAACTTCCC+AGG - tig0019784:83860-83879 Msa1376130:CDS 50.0%
CTCCATCAGAACTCAAGGAG+GGG - tig0019784:83902-83921 Msa1376130:CDS 50.0%
! TTCCCCTCCTTGAGTTCTGA+TGG + tig0019784:83907-83926 None:intergenic 50.0%
! GCGATTTTGTGGCAGGTTGC+CGG - tig0019784:82664-82683 Msa1376130:CDS 55.0%
!! GTTGCCGGTTTGAAATCCGG+TGG - tig0019784:82679-82698 Msa1376130:CDS 55.0%
ATACGGTGAGGGACACTGTC+AGG - tig0019784:82714-82733 Msa1376130:CDS 55.0%
GGACACTGTCAGGATCCCAA+AGG - tig0019784:82724-82743 Msa1376130:CDS 55.0%
CACTGTCAGGATCCCAAAGG+GGG - tig0019784:82727-82746 Msa1376130:CDS 55.0%
CTCATTCCTTGGACCCCCTT+TGG + tig0019784:82743-82762 None:intergenic 55.0%
! AGAGCTTGGTTCGACGGAGA+TGG - tig0019784:83080-83099 Msa1376130:CDS 55.0%
GAGATGGTGCCTGAAGAGCA+AGG - tig0019784:83096-83115 Msa1376130:CDS 55.0%
TGCTACCTTAGCCTGTCCAG+AGG + tig0019784:83160-83179 None:intergenic 55.0%
TGGGCACGAGTTAAGCTTGG+GGG - tig0019784:83191-83210 Msa1376130:CDS 55.0%
GAAGACTCCTCCTCACCTCT+TGG - tig0019784:83447-83466 Msa1376130:CDS 55.0%
CAGTGGTTCCTCTCGTCCAT+TGG + tig0019784:83511-83530 None:intergenic 55.0%
TGAAGCGGAGAGGCAAAGAC+GGG - tig0019784:83539-83558 Msa1376130:CDS 55.0%
! GCGATTTTGTGGCAGGTTGC+CGG - tig0019784:82664-82683 Msa1376130:CDS 55.0%
!! GTTGCCGGTTTGAAATCCGG+TGG - tig0019784:82679-82698 Msa1376130:CDS 55.0%
ATACGGTGAGGGACACTGTC+AGG - tig0019784:82714-82733 Msa1376130:CDS 55.0%
GGACACTGTCAGGATCCCAA+AGG - tig0019784:82724-82743 Msa1376130:CDS 55.0%
CACTGTCAGGATCCCAAAGG+GGG - tig0019784:82727-82746 Msa1376130:CDS 55.0%
CTCATTCCTTGGACCCCCTT+TGG + tig0019784:82743-82762 None:intergenic 55.0%
! AGAGCTTGGTTCGACGGAGA+TGG - tig0019784:83080-83099 Msa1376130:CDS 55.0%
GAGATGGTGCCTGAAGAGCA+AGG - tig0019784:83096-83115 Msa1376130:CDS 55.0%
TGCTACCTTAGCCTGTCCAG+AGG + tig0019784:83160-83179 None:intergenic 55.0%
TGGGCACGAGTTAAGCTTGG+GGG - tig0019784:83191-83210 Msa1376130:CDS 55.0%
GAAGACTCCTCCTCACCTCT+TGG - tig0019784:83447-83466 Msa1376130:CDS 55.0%
CAGTGGTTCCTCTCGTCCAT+TGG + tig0019784:83511-83530 None:intergenic 55.0%
TGAAGCGGAGAGGCAAAGAC+GGG - tig0019784:83539-83558 Msa1376130:CDS 55.0%
AGGATCCCAAAGGGGGTCCA+AGG - tig0019784:82734-82753 Msa1376130:CDS 60.0%
AGGGGGTCCAAGGAATGAGC+AGG - tig0019784:82744-82763 Msa1376130:CDS 60.0%
GGGTGTAGGAGACAGCAACG+AGG - tig0019784:83341-83360 Msa1376130:CDS 60.0%
CTCGTCTCCAAGAGGTGAGG+AGG + tig0019784:83457-83476 None:intergenic 60.0%
CCTCACCTCTTGGAGACGAG+CGG - tig0019784:83457-83476 Msa1376130:CDS 60.0%
GGTTCCTCTCGTCCATTGGC+AGG + tig0019784:83507-83526 None:intergenic 60.0%
CTGCCCCTTGGATATCCACC+CGG - tig0019784:83788-83807 Msa1376130:CDS 60.0%
AGGATCCCAAAGGGGGTCCA+AGG - tig0019784:82734-82753 Msa1376130:CDS 60.0%
AGGGGGTCCAAGGAATGAGC+AGG - tig0019784:82744-82763 Msa1376130:CDS 60.0%
GGGTGTAGGAGACAGCAACG+AGG - tig0019784:83341-83360 Msa1376130:CDS 60.0%
CTCGTCTCCAAGAGGTGAGG+AGG + tig0019784:83457-83476 None:intergenic 60.0%
CCTCACCTCTTGGAGACGAG+CGG - tig0019784:83457-83476 Msa1376130:CDS 60.0%
GGTTCCTCTCGTCCATTGGC+AGG + tig0019784:83507-83526 None:intergenic 60.0%
CTGCCCCTTGGATATCCACC+CGG - tig0019784:83788-83807 Msa1376130:CDS 60.0%
GCTGTCTCCTACACCCCCGT+TGG + tig0019784:83337-83356 None:intergenic 65.0%
CCGCTCGTCTCCAAGAGGTG+AGG + tig0019784:83460-83479 None:intergenic 65.0%
GGCTTCCGCTCGTCTCCAAG+AGG + tig0019784:83465-83484 None:intergenic 65.0%
GCTGTCTCCTACACCCCCGT+TGG + tig0019784:83337-83356 None:intergenic 65.0%
CCGCTCGTCTCCAAGAGGTG+AGG + tig0019784:83460-83479 None:intergenic 65.0%
GGCTTCCGCTCGTCTCCAAG+AGG + tig0019784:83465-83484 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0019784 gene 82469 83980 82469 ID=Msa1376130;Name=Msa1376130
tig0019784 mRNA 82469 83980 82469 ID=Msa1376130-mRNA-1;Parent=Msa1376130;Name=Msa1376130-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|-1|0|1|-1|1|1|0|503
tig0019784 exon 82469 83980 82469 ID=Msa1376130-mRNA-1:exon:4954;Parent=Msa1376130-mRNA-1
tig0019784 CDS 82469 83980 82469 ID=Msa1376130-mRNA-1:cds;Parent=Msa1376130-mRNA-1
Gene Sequence

>Msa1376130

ATGGCTGGTGAGAGGTTTTGTAAGAATGAAGAAGACAAGGCTATGTTGGAATCTGTGTTGGGTAATGAAGCTTTTGCATTTTTTAGCTCAGCTGTTTCAAAACATGTTTTTTCCGATGTTATTGTTCCGCCGAATCTTGACGTGACTATCCACAAAAGGTTGTGTCACATTGTTAACGGTTCCAAGTGGAACTACGCGATTTTGTGGCAGGTTGCCGGTTTGAAATCCGGTGGCTATGTGTTGAAATACGGTGAGGGACACTGTCAGGATCCCAAAGGGGGTCCAAGGAATGAGCAGGAAAGGGAGAAAGATGATGTTAGGAGGAAGGTTTTGGGGAAGATTCATGGTTGCTGGGGTGGATCTAGCTCAGTTGAGAATATTTATAAGAAATTGGACAGTGTTTCGGATTTGTATCTGCTTTACTTGACCTCTGTCTATTATGTGTTTGGTTTTAATTCTCAGTATGGTCCTGGAAATTCTTTTAAGTGTGCTAAACCAAGTTGGTCTTCTGATGCTGGTAGTTGTTTAAATCAGTATGAATCGAGGTCGTTTCTGGCTAAATCGGCTGGTTTTCAAAGTGTTGCTTTTGTACCTCTTAAAGCCGGGGTTATAGAGCTTGGTTCGACGGAGATGGTGCCTGAAGAGCAAGGTTTTTTGGATATGGTCAGGGCAACTTTTGGAGAATCTACCTCTGGACAGGCTAAGGTAGCACCAAAGATTTTTGGGCACGAGTTAAGCTTGGGGGATACAAAATCTCAGTCGATAACTATTAGTTTCTCCCCAAAAGTGGAAGATGATTCTGGTTTCACTTCTGACTCTTACGAAGTACAAGCGCTTGGCGCAAACCATGCTTATGGTAATTCTTCCAACGGGGGTGTAGGAGACAGCAACGAGGCAAAAAAGTACCCTCAGTTGGGTCAAATGATTCCTGGGAATTTTACTTCTCAGGCAAGAGTTTCTTCTATTGATTTAGGTAATGAAGACTCCTCCTCACCTCTTGGAGACGAGCGGAAGCCTAGAAAAAGAGGGAGAAAACCTGCCAATGGACGAGAGGAACCACTGAATCATGTTGAAGCGGAGAGGCAAAGACGGGAGAAGCTTAACCAGAGATTTTACGCCTTAAGAGCTGTTGTTCCTAACATTTCTAAAATGGACAAGGCTTCTCTTCTTGGTGATGCTATTACCCATATCACTGATCTCCAGAAGAAGATCAAGCTTATGGAAACTGAAAAAAACATGGCTGACAATAAGGGCCAGCAATTGCCATTGCAAGATATTGATTTTCAGGCTCGACAGGATGATGCAGTTGTTAGAGTTAGCTGCCCCTTGGATATCCACCCGGTTTCTGGAATTGTTAAAGTGTTCAGAGAGCATCAAATTGTAGCTCAAGAGGCCAATGTTTCAACTTCCCAGGATAAAGTAATTCATACATTCTCCATCAGAACTCAAGGAGGGGAAGCAGCTGCTATACAGTTGAAAGAAAAGCTTGAAGCATCTTTAGCCAAAAATTAA
Protein sequence

>Msa1376130

MAGERFCKNEEDKAMLESVLGNEAFAFFSSAVSKHVFSDVIVPPNLDVTIHKRLCHIVNGSKWNYAILWQVAGLKSGGYVLKYGEGHCQDPKGGPRNEQEREKDDVRRKVLGKIHGCWGGSSSVENIYKKLDSVSDLYLLYLTSVYYVFGFNSQYGPGNSFKCAKPSWSSDAGSCLNQYESRSFLAKSAGFQSVAFVPLKAGVIELGSTEMVPEEQGFLDMVRATFGESTSGQAKVAPKIFGHELSLGDTKSQSITISFSPKVEDDSGFTSDSYEVQALGANHAYGNSSNGGVGDSNEAKKYPQLGQMIPGNFTSQARVSSIDLGNEDSSSPLGDERKPRKRGRKPANGREEPLNHVEAERQRREKLNQRFYALRAVVPNISKMDKASLLGDAITHITDLQKKIKLMETEKNMADNKGQQLPLQDIDFQARQDDAVVRVSCPLDIHPVSGIVKVFREHQIVAQEANVSTSQDKVIHTFSIRTQGGEAAAIQLKEKLEASLAKN*