Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1407040 | XP_003597638.2 | 91.742 | 884 | 71 | 2 | 1 | 883 | 1 | 883 | 0.0 | 1665 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1407040 | sp|O65924|Y2921_ARATH | 48.399 | 843 | 408 | 12 | 18 | 846 | 21 | 850 | 0.0 | 789 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000800 | Msa1407040 | 0.812641 | 3.827540e-51 | -8.615850e-47 |
| Msa0001510 | Msa1407040 | -0.804510 | 2.086657e-49 | -8.615850e-47 |
| Msa0002690 | Msa1407040 | -0.821582 | 3.739063e-53 | -8.615850e-47 |
| Msa0002700 | Msa1407040 | -0.816648 | 4.961796e-52 | -8.615850e-47 |
| Msa0004180 | Msa1407040 | -0.855392 | 6.508088e-62 | -8.615850e-47 |
| Msa0007810 | Msa1407040 | -0.814198 | 1.740499e-51 | -8.615850e-47 |
| Msa0009840 | Msa1407040 | 0.800627 | 1.319079e-48 | -8.615850e-47 |
| Msa0011010 | Msa1407040 | 0.805611 | 1.227707e-49 | -8.615850e-47 |
| Msa0011040 | Msa1407040 | 0.808877 | 2.495933e-50 | -8.615850e-47 |
| Msa0011060 | Msa1407040 | -0.833565 | 4.956504e-56 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1407040 | MtrunA17_Chr2g0330591 | 91.742 | 884 | 71 | 2 | 1 | 883 | 1 | 883 | 0.0 | 1665 |
| Msa1407040 | MtrunA17_Chr8g0341011 | 47.545 | 835 | 415 | 13 | 19 | 846 | 25 | 843 | 0.0 | 770 |
| Msa1407040 | MtrunA17_Chr8g0341151 | 46.961 | 839 | 417 | 12 | 18 | 846 | 13 | 833 | 0.0 | 767 |
| Msa1407040 | MtrunA17_Chr8g0340931 | 45.921 | 858 | 431 | 13 | 1 | 846 | 3 | 839 | 0.0 | 758 |
| Msa1407040 | MtrunA17_Chr8g0340881 | 46.643 | 849 | 425 | 15 | 8 | 846 | 14 | 844 | 0.0 | 748 |
| Msa1407040 | MtrunA17_Chr8g0340891 | 46.441 | 857 | 422 | 16 | 1 | 846 | 8 | 838 | 0.0 | 740 |
| Msa1407040 | MtrunA17_Chr8g0347661 | 46.341 | 861 | 427 | 17 | 1 | 846 | 5 | 845 | 0.0 | 740 |
| Msa1407040 | MtrunA17_Chr8g0340911 | 46.316 | 855 | 430 | 14 | 1 | 846 | 5 | 839 | 0.0 | 736 |
| Msa1407040 | MtrunA17_Chr6g0487571 | 45.675 | 867 | 429 | 12 | 7 | 846 | 4 | 855 | 0.0 | 726 |
| Msa1407040 | MtrunA17_Chr8g0340871 | 45.721 | 853 | 436 | 13 | 4 | 846 | 13 | 848 | 0.0 | 721 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1407040 | AT4G29990.1 | 50.519 | 867 | 402 | 18 | 4 | 857 | 5 | 857 | 0.0 | 830 |
| Msa1407040 | AT2G19210.1 | 48.399 | 843 | 408 | 12 | 18 | 846 | 21 | 850 | 0.0 | 789 |
| Msa1407040 | AT1G05700.1 | 45.444 | 856 | 443 | 10 | 4 | 849 | 5 | 846 | 0.0 | 753 |
| Msa1407040 | AT2G19230.1 | 46.956 | 854 | 425 | 13 | 5 | 846 | 6 | 843 | 0.0 | 753 |
| Msa1407040 | AT2G19230.2 | 47.073 | 854 | 423 | 13 | 5 | 846 | 6 | 842 | 0.0 | 750 |
| Msa1407040 | AT1G51800.1 | 44.582 | 886 | 449 | 14 | 1 | 859 | 1 | 871 | 0.0 | 746 |
| Msa1407040 | AT1G05700.2 | 44.444 | 882 | 445 | 12 | 4 | 849 | 5 | 877 | 0.0 | 744 |
| Msa1407040 | AT1G51860.1 | 45.505 | 901 | 426 | 20 | 9 | 871 | 10 | 883 | 0.0 | 729 |
| Msa1407040 | AT1G51880.1 | 46.395 | 860 | 416 | 18 | 10 | 848 | 7 | 842 | 0.0 | 728 |
| Msa1407040 | AT1G51880.2 | 46.744 | 860 | 413 | 20 | 10 | 848 | 25 | 860 | 0.0 | 728 |
Find 186 sgRNAs with CRISPR-Local
Find 342 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACGGTAATATATTGAGT+TGG | 0.538228 | tig0023257:-2935 | Msa1407040:CDS |
| AAAAGTGATGTATTCAGTTT+TGG | 0.224895 | tig0023257:-2277 | Msa1407040:CDS |
| AAATACTTGACCAGTCTTAT+AGG | 0.240601 | tig0023257:-3307 | Msa1407040:CDS |
| AAATTGCAGATATCCTTGAG+CGG | 0.682985 | tig0023257:+3480 | None:intergenic |
| AACAATACCTGATAAATGGT+TGG | 0.520110 | tig0023257:+3222 | None:intergenic |
| AACATCCCTAGTGTAATAGA+TGG | 0.436617 | tig0023257:+6867 | None:intergenic |
| AACCATGAGGCATGTAGTTA+TGG | 0.434194 | tig0023257:-2041 | Msa1407040:CDS |
| AAGGACTTACAAATATTCTA+TGG | 0.304937 | tig0023257:+5220 | None:intergenic |
| AAGTAAGAAACTGAAGTACA+AGG | 0.624424 | tig0023257:+5480 | None:intergenic |
| AATCCAGATCTTTGCTCCTC+AGG | 0.404497 | tig0023257:-4086 | Msa1407040:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTCTGCATATAGACTACAT+AGG | - | tig0023257:1873-1892 | Msa1407040:three_prime_UTR | 30.0% | |
| ACACAAGTTCTTAACTATGA+AGG | + | tig0023257:1987-2006 | None:intergenic | 30.0% | |
| GATGTTAATTTCACAGATAC+TGG | - | tig0023257:2099-2118 | Msa1407040:CDS | 30.0% | |
| ATTCCAAAACTGTCTTTCAA+GGG | + | tig0023257:2160-2179 | None:intergenic | 30.0% | |
| GTAGCAAGAAATATTTAGTC+CGG | - | tig0023257:2238-2257 | Msa1407040:CDS | 30.0% | |
| TAGCAAGAAATATTTAGTCC+GGG | - | tig0023257:2239-2258 | Msa1407040:CDS | 30.0% | |
| CCAAGATAGATATCAAATTC+AGG | + | tig0023257:2306-2325 | None:intergenic | 30.0% | |
| CCTGAATTTGATATCTATCT+TGG | - | tig0023257:2303-2322 | Msa1407040:CDS | 30.0% | |
| ATATCTATCTTGGAGTCAAA+TGG | - | tig0023257:2313-2332 | Msa1407040:intron | 30.0% | |
| TTCATCTAGTGTAATAACCA+AGG | - | tig0023257:2359-2378 | Msa1407040:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0023257 | gene | 1763 | 7208 | 1763 | ID=Msa1407040;Name=Msa1407040 |
| tig0023257 | mRNA | 1763 | 7208 | 1763 | ID=Msa1407040-mRNA-1;Parent=Msa1407040;Name=Msa1407040-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0.33|0.30|1|1|1|13|147|883 |
| tig0023257 | exon | 1763 | 2327 | 1763 | ID=Msa1407040-mRNA-1:exon:24908;Parent=Msa1407040-mRNA-1 |
| tig0023257 | exon | 2593 | 2782 | 2593 | ID=Msa1407040-mRNA-1:exon:24907;Parent=Msa1407040-mRNA-1 |
| tig0023257 | exon | 2898 | 2960 | 2898 | ID=Msa1407040-mRNA-1:exon:24906;Parent=Msa1407040-mRNA-1 |
| tig0023257 | exon | 3230 | 3356 | 3230 | ID=Msa1407040-mRNA-1:exon:24905;Parent=Msa1407040-mRNA-1 |
| tig0023257 | exon | 3471 | 3706 | 3471 | ID=Msa1407040-mRNA-1:exon:24904;Parent=Msa1407040-mRNA-1 |
| tig0023257 | exon | 3964 | 4118 | 3964 | ID=Msa1407040-mRNA-1:exon:24903;Parent=Msa1407040-mRNA-1 |
| tig0023257 | exon | 4303 | 4383 | 4303 | ID=Msa1407040-mRNA-1:exon:24902;Parent=Msa1407040-mRNA-1 |
| tig0023257 | exon | 5054 | 5125 | 5054 | ID=Msa1407040-mRNA-1:exon:24901;Parent=Msa1407040-mRNA-1 |
| tig0023257 | exon | 5230 | 5301 | 5230 | ID=Msa1407040-mRNA-1:exon:24900;Parent=Msa1407040-mRNA-1 |
| tig0023257 | exon | 5423 | 5561 | 5423 | ID=Msa1407040-mRNA-1:exon:24899;Parent=Msa1407040-mRNA-1 |
| tig0023257 | exon | 5690 | 6168 | 5690 | ID=Msa1407040-mRNA-1:exon:24898;Parent=Msa1407040-mRNA-1 |
| tig0023257 | exon | 6412 | 6949 | 6412 | ID=Msa1407040-mRNA-1:exon:24897;Parent=Msa1407040-mRNA-1 |
| tig0023257 | exon | 7127 | 7208 | 7127 | ID=Msa1407040-mRNA-1:exon:24896;Parent=Msa1407040-mRNA-1 |
| tig0023257 | CDS | 7127 | 7208 | 7127 | ID=Msa1407040-mRNA-1:cds;Parent=Msa1407040-mRNA-1 |
| tig0023257 | CDS | 6412 | 6949 | 6412 | ID=Msa1407040-mRNA-1:cds;Parent=Msa1407040-mRNA-1 |
| tig0023257 | CDS | 5690 | 6168 | 5690 | ID=Msa1407040-mRNA-1:cds;Parent=Msa1407040-mRNA-1 |
| tig0023257 | CDS | 5423 | 5561 | 5423 | ID=Msa1407040-mRNA-1:cds;Parent=Msa1407040-mRNA-1 |
| tig0023257 | CDS | 5230 | 5301 | 5230 | ID=Msa1407040-mRNA-1:cds;Parent=Msa1407040-mRNA-1 |
| tig0023257 | CDS | 5054 | 5125 | 5054 | ID=Msa1407040-mRNA-1:cds;Parent=Msa1407040-mRNA-1 |
| tig0023257 | CDS | 4303 | 4383 | 4303 | ID=Msa1407040-mRNA-1:cds;Parent=Msa1407040-mRNA-1 |
| tig0023257 | CDS | 3964 | 4118 | 3964 | ID=Msa1407040-mRNA-1:cds;Parent=Msa1407040-mRNA-1 |
| tig0023257 | CDS | 3471 | 3706 | 3471 | ID=Msa1407040-mRNA-1:cds;Parent=Msa1407040-mRNA-1 |
| tig0023257 | CDS | 3230 | 3356 | 3230 | ID=Msa1407040-mRNA-1:cds;Parent=Msa1407040-mRNA-1 |
| tig0023257 | CDS | 2898 | 2960 | 2898 | ID=Msa1407040-mRNA-1:cds;Parent=Msa1407040-mRNA-1 |
| tig0023257 | CDS | 2593 | 2782 | 2593 | ID=Msa1407040-mRNA-1:cds;Parent=Msa1407040-mRNA-1 |
| tig0023257 | CDS | 1910 | 2327 | 1910 | ID=Msa1407040-mRNA-1:cds;Parent=Msa1407040-mRNA-1 |
| tig0023257 | three_prime_UTR | 1763 | 1909 | 1763 | ID=Msa1407040-mRNA-1:three_prime_utr;Parent=Msa1407040-mRNA-1 |
| Gene Sequence |
>Msa1407040 ATGGCAAGACGCTTGCTATCTATAGTGTTTCTACTTTCTTTGACTCTTCCTTTCATTGTTCATGCTCAGAACAACCAATCAGGCTTCATTAGTATTGACTGTGGACTAGTGGATGAACCACGCTACACAGATGAAACCACTTCCATCTATTACACTAGGGATGTTAATTTCACAGATACTGGTGTAAGCCACAGCATATCATCTAAACTCAAGGCATCCCTTGAAAGACAGTTTTGGAATGTACGAAGCTTCCCAGAAGGAACAAGGAACTGCTACACGCTTTATGTTTCACATGGAAGTAGCAAGAAATATTTAGTCCGGGCCAGTTTTGTGTATGGTAATTATGATGGAAAAGATTCTCTTCCTGAATTTGATATCTATCTTGGAGTCAAATGGTGGGAATCAGTGGTGTTTGAGAATTCATCTAGTGTAATAACCAAGGAAATCATTTATGCTGCATCCTCAGATTATGTTCATGTTTGTTTGTTTAATACTGGCAAGGGTACACCTTTTATTTCAGTTTTAGAGCTTAGAGTTCTTAAAAGTGATGCTTATCTTGATAACTCTTTGGAGCTTTTGGGGAGGTTTGATATAGGCTCAAAGGATGATAAAAAAATCAGATATCCAGATGATATTTATGACAGAACATGGACACCTTATAACTCAATTGAATGGAAAAAAATAGACACTTCACTCACCATAGACCAAAGAGCACCTCCATTCGACTTTTTTACGGAACCACCCTCCACTGTCATGAGAACAACAGCAATTCCAGCAAATTCCAGTGACAACATGGAATTTTTCTTCCTTCCCAAGTACAATTCCTCCAGATATTATGTGTACGTGTACTTTGCTGAAATCCAGAAGCTCCAGGCAAATCAAATCAGAGAGTTCAACATTTTTGTAAATGGGAATCTTCTAAGTAGTGAAGTCAATCCTTTGTATCTTAAAAACGGGTATTATGTATTAGCTAGAGGTGAAACTAAGTTGGAAATTTGGCTTAATAAGACTAGTAGATCAACTCTTCCACCGCTATTCAATGCCGTCGAGATATATATGGTAAAGGACTTCCTACAATCAGAAACATATCAAACCGATGTTGATGCCATCCTAAATGTCAAGTCAATTTATGGGATCAAGAGAAACTGGCAAGGAGATCCTTGTACTTCAGTTTCTTACTTGTGGAATGGTTTAAACTGCAGCTATGCTGGTACTGATTCACCAAGGATCATATACCTGAACTTAACTTCCAGCGGTTTAATTGGAACTATAGCTGCCGGCATATCCAATCTGAAGTCCATAGAATATTTGGATTTGTCAAATAACAATTTGACTGGAGCTGTGCCTGATTTCCTATCTCAGCTGCGTTTCTTGAGAGTTCTAAATCTTCAAGGAAATCAGTTATCAGGAGCAATTCCATTACAACTCCTTGTGCGTTCAGAGAATAGTACGCTCCAATTCAATTTTGGTGGAAATCCAGATCTTTGCTCCTCAGGTTCATGCAACAAATGGAATGGGAATAAGGTTGTAGTTCCATTGGTAACATCAATTGGTGGAGCTTTCTTGATACTAGCAGTAGCAGTGATTTCTTTTCGCATTTACAATAAGAGAAACCGAGTTTCCCATGAGGTGATCAAGCTGGGTGCTAATTCAAGAATAAAGCAGGAACTAGAGTCAAAGAAACAAGAATTCACCTATGAAGAAGTATTAAGAACTACCAGAAACTTTGAAAAGATTCTAGGGAAAGGAGCATCTGGAACAGTATACCATGGCTGGATTGATCAAAATACTGAGGTAGCTGTCAAAATGCTATCTTCCTCATCCGCTCAAGGATATCTGCAATTTCAAGCAGAGGCCAAATTTTTTGCTACAGTTCATCACAAATACTTGACCAGTCTTATAGGTTACTGTGATGATGGCACCAATATGGCTCTTATCTATGAGTATATGGCTAATGGAGACTTATCCAACCATTTATCAGATAAAAACGGTAATATATTGAGTTGGAATCAAAGACTTCAAATTGCAGTGGATACTGCAGAAGGATTGGAGTATCTGCATCATGGTTGCAATCCACCAATTGTTCATAGAGATGTTAAGTCTAAAAACATCTTACTAAATGAAAAATTACAAGGTAAATTAGCTGATTTTGGATTGTCCAAGATCTTTCCTAATGAAGGTGAAACTCATCTCTCCACAGCTATTGCTGGCACGCCTGGATACCTCGACCCGGAGTACAATAGATTAAGCAGGTTGAGAGAAAAAAGTGATGTATTCAGTTTTGGGGTGGTTTTGCTGGAGATAATTACAGGACAACCTGCCATAACCAAAACTGAAAACAAAATCCATATTGTTCAATTGGTTGGTGACATGCTGCTGGAAAGGGAGGTAAAAGATATTGTTGATCCCAGATTGCAGGGAGACTTTGATATTAACTATGCAACTAAAGCTTTGGATACCGCAATGGCTTGTGTAGCACAATCTTCCATCAATAGACCAACCATGAGGCATGTAGTTATGGAACTGAAACAATGTTTGGAAAACAAGATCACTTATCCGTCTGACTCCAAATACACTCTTGAGAGTTTACCTAGTGCATTATATTCTGTCTCCTTTGATAGAATCAGTGGCGAGAGCTCTCTAGCGAGGTAG |
| Protein sequence |
>Msa1407040 MARRLLSIVFLLSLTLPFIVHAQNNQSGFISIDCGLVDEPRYTDETTSIYYTRDVNFTDTGVSHSISSKLKASLERQFWNVRSFPEGTRNCYTLYVSHGSSKKYLVRASFVYGNYDGKDSLPEFDIYLGVKWWESVVFENSSSVITKEIIYAASSDYVHVCLFNTGKGTPFISVLELRVLKSDAYLDNSLELLGRFDIGSKDDKKIRYPDDIYDRTWTPYNSIEWKKIDTSLTIDQRAPPFDFFTEPPSTVMRTTAIPANSSDNMEFFFLPKYNSSRYYVYVYFAEIQKLQANQIREFNIFVNGNLLSSEVNPLYLKNGYYVLARGETKLEIWLNKTSRSTLPPLFNAVEIYMVKDFLQSETYQTDVDAILNVKSIYGIKRNWQGDPCTSVSYLWNGLNCSYAGTDSPRIIYLNLTSSGLIGTIAAGISNLKSIEYLDLSNNNLTGAVPDFLSQLRFLRVLNLQGNQLSGAIPLQLLVRSENSTLQFNFGGNPDLCSSGSCNKWNGNKVVVPLVTSIGGAFLILAVAVISFRIYNKRNRVSHEVIKLGANSRIKQELESKKQEFTYEEVLRTTRNFEKILGKGASGTVYHGWIDQNTEVAVKMLSSSSAQGYLQFQAEAKFFATVHHKYLTSLIGYCDDGTNMALIYEYMANGDLSNHLSDKNGNILSWNQRLQIAVDTAEGLEYLHHGCNPPIVHRDVKSKNILLNEKLQGKLADFGLSKIFPNEGETHLSTAIAGTPGYLDPEYNRLSRLREKSDVFSFGVVLLEIITGQPAITKTENKIHIVQLVGDMLLEREVKDIVDPRLQGDFDINYATKALDTAMACVAQSSINRPTMRHVVMELKQCLENKITYPSDSKYTLESLPSALYSVSFDRISGESSLAR* |
