Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1208830 | XP_013457733.1 | 95.146 | 206 | 10 | 0 | 1 | 206 | 1 | 206 | 9.18e-146 | 414 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1208830 | sp|Q9FGS5|NRT31_ARATH | 51.196 | 209 | 91 | 5 | 1 | 202 | 1 | 205 | 8.50e-64 | 199 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004940 | Msa1208830 | 0.834016 | 3.823674e-56 | -8.615850e-47 |
| Msa0004990 | Msa1208830 | 0.813267 | 2.790694e-51 | -8.615850e-47 |
| Msa0031650 | Msa1208830 | 0.831915 | 1.273581e-55 | -8.615850e-47 |
| Msa0034800 | Msa1208830 | -0.800502 | 1.398675e-48 | -8.615850e-47 |
| Msa0053040 | Msa1208830 | 0.808636 | 2.809919e-50 | -8.615850e-47 |
| Msa0059420 | Msa1208830 | 0.803455 | 3.458051e-49 | -8.615850e-47 |
| Msa0059880 | Msa1208830 | 0.818349 | 2.053496e-52 | -8.615850e-47 |
| Msa0064990 | Msa1208830 | 0.800433 | 1.444830e-48 | -8.615850e-47 |
| Msa0093530 | Msa1208830 | 0.821357 | 4.214419e-53 | -8.615850e-47 |
| Msa0098380 | Msa1208830 | 0.823178 | 1.593000e-53 | -8.615850e-47 |
| Msa0107830 | Msa1208830 | 0.826442 | 2.707027e-54 | -8.615850e-47 |
| Msa0604280 | Msa1208830 | 0.811693 | 6.162686e-51 | -8.615850e-47 |
| Msa0628200 | Msa1208830 | 0.824970 | 6.046107e-54 | -8.615850e-47 |
| Msa0629030 | Msa1208830 | 0.818101 | 2.336116e-52 | -8.615850e-47 |
| Msa0630130 | Msa1208830 | 0.828704 | 7.755356e-55 | -8.615850e-47 |
| Msa0633070 | Msa1208830 | 0.831513 | 1.599637e-55 | -8.615850e-47 |
| Msa0634290 | Msa1208830 | 0.811754 | 5.977711e-51 | -8.615850e-47 |
| Msa0637590 | Msa1208830 | 0.813684 | 2.259589e-51 | -8.615850e-47 |
| Msa0637700 | Msa1208830 | 0.802978 | 4.340878e-49 | -8.615850e-47 |
| Msa0639400 | Msa1208830 | 0.808798 | 2.595119e-50 | -8.615850e-47 |
| Msa0639410 | Msa1208830 | 0.821007 | 5.076698e-53 | -8.615850e-47 |
| Msa0645120 | Msa1208830 | 0.801103 | 1.054325e-48 | -8.615850e-47 |
| Msa0651820 | Msa1208830 | 0.817838 | 2.679371e-52 | -8.615850e-47 |
| Msa0657260 | Msa1208830 | 0.829638 | 4.603050e-55 | -8.615850e-47 |
| Msa0659100 | Msa1208830 | -0.805604 | 1.232190e-49 | -8.615850e-47 |
| Msa0666580 | Msa1208830 | 0.813840 | 2.087332e-51 | -8.615850e-47 |
| Msa0668210 | Msa1208830 | 0.825346 | 4.927301e-54 | -8.615850e-47 |
| Msa0668230 | Msa1208830 | 0.810629 | 1.048022e-50 | -8.615850e-47 |
| Msa0682420 | Msa1208830 | 0.803210 | 3.885894e-49 | -8.615850e-47 |
| Msa0701490 | Msa1208830 | 0.827029 | 1.960172e-54 | -8.615850e-47 |
| Msa0701560 | Msa1208830 | 0.828510 | 8.637236e-55 | -8.615850e-47 |
| Msa0715260 | Msa1208830 | 0.822022 | 2.957642e-53 | -8.615850e-47 |
| Msa0718770 | Msa1208830 | 0.811941 | 5.441787e-51 | -8.615850e-47 |
| Msa0721260 | Msa1208830 | 0.840823 | 6.895405e-58 | -8.615850e-47 |
| Msa0728520 | Msa1208830 | 0.856225 | 3.714945e-62 | -8.615850e-47 |
| Msa0743370 | Msa1208830 | 0.800884 | 1.168668e-48 | -8.615850e-47 |
| Msa0744550 | Msa1208830 | 0.803631 | 3.178867e-49 | -8.615850e-47 |
| Msa0747710 | Msa1208830 | 0.819597 | 1.068191e-52 | -8.615850e-47 |
| Msa0750790 | Msa1208830 | 0.803639 | 3.167155e-49 | -8.615850e-47 |
| Msa0753410 | Msa1208830 | 0.826261 | 2.989453e-54 | -8.615850e-47 |
| Msa0754530 | Msa1208830 | 0.808933 | 2.427956e-50 | -8.615850e-47 |
| Msa0757240 | Msa1208830 | 0.821494 | 3.919328e-53 | -8.615850e-47 |
| Msa0776540 | Msa1208830 | 0.858596 | 7.390441e-63 | -8.615850e-47 |
| Msa0777600 | Msa1208830 | 0.806056 | 9.898330e-50 | -8.615850e-47 |
| Msa0783580 | Msa1208830 | 0.812826 | 3.486664e-51 | -8.615850e-47 |
| Msa0795320 | Msa1208830 | 0.821423 | 4.069650e-53 | -8.615850e-47 |
| Msa0795330 | Msa1208830 | 0.824554 | 7.579018e-54 | -8.615850e-47 |
| Msa0798230 | Msa1208830 | 0.827729 | 1.331857e-54 | -8.615850e-47 |
| Msa0812050 | Msa1208830 | 0.800479 | 1.413431e-48 | -8.615850e-47 |
| Msa0814660 | Msa1208830 | 0.817980 | 2.488443e-52 | -8.615850e-47 |
| Msa0826360 | Msa1208830 | 0.821197 | 4.588927e-53 | -8.615850e-47 |
| Msa0827800 | Msa1208830 | 0.804220 | 2.398275e-49 | -8.615850e-47 |
| Msa0833230 | Msa1208830 | 0.815376 | 9.541292e-52 | -8.615850e-47 |
| Msa0836740 | Msa1208830 | 0.820440 | 6.852008e-53 | -8.615850e-47 |
| Msa0837550 | Msa1208830 | 0.808512 | 2.986596e-50 | -8.615850e-47 |
| Msa0126960 | Msa1208830 | 0.819951 | 8.867266e-53 | -8.615850e-47 |
| Msa0150910 | Msa1208830 | 0.813734 | 2.203090e-51 | -8.615850e-47 |
| Msa0171740 | Msa1208830 | 0.841336 | 5.054252e-58 | -8.615850e-47 |
| Msa0185040 | Msa1208830 | 0.808336 | 3.256811e-50 | -8.615850e-47 |
| Msa0196420 | Msa1208830 | 0.812112 | 4.994011e-51 | -8.615850e-47 |
| Msa0199860 | Msa1208830 | 0.819158 | 1.345098e-52 | -8.615850e-47 |
| Msa0200090 | Msa1208830 | 0.800398 | 1.468447e-48 | -8.615850e-47 |
| Msa0202650 | Msa1208830 | 0.804280 | 2.330191e-49 | -8.615850e-47 |
| Msa0207930 | Msa1208830 | 0.829550 | 4.833957e-55 | -8.615850e-47 |
| Msa0207970 | Msa1208830 | 0.870675 | 1.220248e-66 | -8.615850e-47 |
| Msa0234120 | Msa1208830 | 0.818399 | 2.000587e-52 | -8.615850e-47 |
| Msa0238330 | Msa1208830 | 0.810810 | 9.579259e-51 | -8.615850e-47 |
| Msa0390200 | Msa1208830 | 0.821023 | 5.031759e-53 | -8.615850e-47 |
| Msa0409490 | Msa1208830 | 0.806619 | 7.532188e-50 | -8.615850e-47 |
| Msa0414480 | Msa1208830 | 0.803297 | 3.728554e-49 | -8.615850e-47 |
| Msa0418150 | Msa1208830 | 0.803291 | 3.738352e-49 | -8.615850e-47 |
| Msa0421080 | Msa1208830 | 0.820916 | 5.325760e-53 | -8.615850e-47 |
| Msa0433090 | Msa1208830 | 0.807773 | 4.290690e-50 | -8.615850e-47 |
| Msa0464300 | Msa1208830 | 0.807571 | 4.735580e-50 | -8.615850e-47 |
| Msa0464310 | Msa1208830 | 0.808567 | 2.906204e-50 | -8.615850e-47 |
| Msa0480540 | Msa1208830 | 0.804602 | 1.996856e-49 | -8.615850e-47 |
| Msa0996980 | Msa1208830 | 0.817217 | 3.697259e-52 | -8.615850e-47 |
| Msa1023430 | Msa1208830 | 0.844511 | 7.226359e-59 | -8.615850e-47 |
| Msa1024350 | Msa1208830 | 0.809919 | 1.491479e-50 | -8.615850e-47 |
| Msa1035050 | Msa1208830 | 0.828055 | 1.111793e-54 | -8.615850e-47 |
| Msa1037360 | Msa1208830 | 0.829637 | 4.605677e-55 | -8.615850e-47 |
| Msa1048390 | Msa1208830 | 0.820945 | 5.244091e-53 | -8.615850e-47 |
| Msa1061670 | Msa1208830 | 0.802383 | 5.757725e-49 | -8.615850e-47 |
| Msa1061840 | Msa1208830 | 0.841677 | 4.109024e-58 | -8.615850e-47 |
| Msa1064940 | Msa1208830 | 0.824025 | 1.009269e-53 | -8.615850e-47 |
| Msa1075090 | Msa1208830 | 0.809939 | 1.476793e-50 | -8.615850e-47 |
| Msa1085820 | Msa1208830 | 0.815579 | 8.600594e-52 | -8.615850e-47 |
| Msa1087380 | Msa1208830 | 0.814571 | 1.439946e-51 | -8.615850e-47 |
| Msa1100330 | Msa1208830 | 0.804737 | 1.871114e-49 | -8.615850e-47 |
| Msa0243970 | Msa1208830 | 0.814239 | 1.704479e-51 | -8.615850e-47 |
| Msa0245660 | Msa1208830 | 0.806862 | 6.694352e-50 | -8.615850e-47 |
| Msa0266640 | Msa1208830 | 0.806166 | 9.387690e-50 | -8.615850e-47 |
| Msa0266650 | Msa1208830 | 0.815071 | 1.115169e-51 | -8.615850e-47 |
| Msa0269710 | Msa1208830 | 0.813763 | 2.171211e-51 | -8.615850e-47 |
| Msa0271700 | Msa1208830 | 0.805369 | 1.380091e-49 | -8.615850e-47 |
| Msa0274260 | Msa1208830 | 0.818034 | 2.418871e-52 | -8.615850e-47 |
| Msa0275730 | Msa1208830 | 0.815352 | 9.661673e-52 | -8.615850e-47 |
| Msa0316230 | Msa1208830 | 0.829132 | 6.108033e-55 | -8.615850e-47 |
| Msa0320130 | Msa1208830 | 0.827355 | 1.637707e-54 | -8.615850e-47 |
| Msa0320140 | Msa1208830 | 0.820507 | 6.613516e-53 | -8.615850e-47 |
| Msa0321750 | Msa1208830 | 0.813358 | 2.665805e-51 | -8.615850e-47 |
| Msa0346170 | Msa1208830 | 0.834475 | 2.933832e-56 | -8.615850e-47 |
| Msa1107510 | Msa1208830 | 0.837126 | 6.246013e-57 | -8.615850e-47 |
| Msa1123000 | Msa1208830 | 0.819912 | 9.051035e-53 | -8.615850e-47 |
| Msa1138110 | Msa1208830 | 0.809837 | 1.553242e-50 | -8.615850e-47 |
| Msa1151070 | Msa1208830 | 0.821377 | 4.171106e-53 | -8.615850e-47 |
| Msa1162430 | Msa1208830 | 0.810480 | 1.128761e-50 | -8.615850e-47 |
| Msa1164230 | Msa1208830 | 0.800589 | 1.342689e-48 | -8.615850e-47 |
| Msa1173290 | Msa1208830 | 0.821187 | 4.613949e-53 | -8.615850e-47 |
| Msa1177090 | Msa1208830 | 0.802755 | 4.825165e-49 | -8.615850e-47 |
| Msa1191270 | Msa1208830 | 0.801224 | 9.959486e-49 | -8.615850e-47 |
| Msa1202110 | Msa1208830 | 0.802985 | 4.326044e-49 | -8.615850e-47 |
| Msa1206330 | Msa1208830 | 0.812880 | 3.393903e-51 | -8.615850e-47 |
| Msa1206380 | Msa1208830 | 0.806101 | 9.684845e-50 | -8.615850e-47 |
| Msa1208830 | Msa1208910 | 0.966019 | 2.298046e-125 | -8.615850e-47 |
| Msa1208830 | Msa1211610 | 0.808882 | 2.489763e-50 | -8.615850e-47 |
| Msa1208830 | Msa1221520 | 0.810067 | 1.385871e-50 | -8.615850e-47 |
| Msa1208830 | Msa1223710 | 0.802847 | 4.620334e-49 | -8.615850e-47 |
| Msa1208830 | Msa1239190 | 0.809692 | 1.668773e-50 | -8.615850e-47 |
| Msa1208830 | Msa1239210 | 0.812183 | 4.819412e-51 | -8.615850e-47 |
| Msa1208830 | Msa1254870 | 0.803613 | 3.207273e-49 | -8.615850e-47 |
| Msa1208830 | Msa1259560 | 0.800862 | 1.180978e-48 | -8.615850e-47 |
| Msa1208830 | Msa1263370 | 0.827981 | 1.158572e-54 | -8.615850e-47 |
| Msa1208830 | Msa1264940 | 0.800744 | 1.248374e-48 | -8.615850e-47 |
| Msa1208830 | Msa1269200 | 0.822942 | 1.808467e-53 | -8.615850e-47 |
| Msa1208830 | Msa1299480 | 0.800758 | 1.240163e-48 | -8.615850e-47 |
| Msa1208830 | Msa1303150 | 0.816748 | 4.713784e-52 | -8.615850e-47 |
| Msa1208830 | Msa1323150 | 0.811410 | 7.098050e-51 | -8.615850e-47 |
| Msa1208830 | Msa1328430 | 0.803874 | 2.830552e-49 | -8.615850e-47 |
| Msa1208830 | Msa1334000 | 0.811937 | 5.453208e-51 | -8.615850e-47 |
| Msa1208830 | Msa1349420 | 0.816526 | 5.285441e-52 | -8.615850e-47 |
| Msa1208830 | Msa1355330 | 0.802390 | 5.740541e-49 | -8.615850e-47 |
| Msa1208830 | Msa1357840 | 0.802019 | 6.841682e-49 | -8.615850e-47 |
| Msa1208830 | Msa1362520 | 0.802125 | 6.506969e-49 | -8.615850e-47 |
| Msa1208830 | Msa1364690 | 0.806345 | 8.605240e-50 | -8.615850e-47 |
| Msa1208830 | Msa1398110 | 0.843993 | 9.956621e-59 | -8.615850e-47 |
| Msa1208830 | Msa1398120 | 0.811399 | 7.138410e-51 | -8.615850e-47 |
| Msa1208830 | Msa1411550 | 0.801183 | 1.015308e-48 | -8.615850e-47 |
| Msa1208830 | Msa1412360 | 0.812588 | 3.931516e-51 | -8.615850e-47 |
| Msa1208830 | Msa1419210 | 0.880729 | 4.317618e-70 | -8.615850e-47 |
| Msa1208830 | Msa1437590 | 0.811054 | 8.480695e-51 | -8.615850e-47 |
| Msa1208830 | Msa1459930 | 0.800779 | 1.228116e-48 | -8.615850e-47 |
| Msa0489370 | Msa1208830 | 0.818573 | 1.826644e-52 | -8.615850e-47 |
| Msa0493710 | Msa1208830 | 0.835915 | 1.270679e-56 | -8.615850e-47 |
| Msa0493870 | Msa1208830 | 0.804503 | 2.093738e-49 | -8.615850e-47 |
| Msa0497680 | Msa1208830 | 0.805498 | 1.296902e-49 | -8.615850e-47 |
| Msa0510870 | Msa1208830 | 0.812229 | 4.709996e-51 | -8.615850e-47 |
| Msa0524710 | Msa1208830 | 0.810663 | 1.030550e-50 | -8.615850e-47 |
| Msa0529420 | Msa1208830 | 0.811763 | 5.951168e-51 | -8.615850e-47 |
| Msa0533510 | Msa1208830 | 0.813759 | 2.175383e-51 | -8.615850e-47 |
| Msa0533520 | Msa1208830 | 0.817208 | 3.715762e-52 | -8.615850e-47 |
| Msa0538440 | Msa1208830 | 0.822082 | 2.865637e-53 | -8.615850e-47 |
| Msa0538450 | Msa1208830 | 0.808716 | 2.701792e-50 | -8.615850e-47 |
| Msa0538530 | Msa1208830 | 0.819410 | 1.178546e-52 | -8.615850e-47 |
| Msa0545680 | Msa1208830 | 0.803094 | 4.106614e-49 | -8.615850e-47 |
| Msa0546760 | Msa1208830 | 0.808586 | 2.880133e-50 | -8.615850e-47 |
| Msa0554660 | Msa1208830 | 0.820414 | 6.947142e-53 | -8.615850e-47 |
| Msa0565120 | Msa1208830 | 0.810096 | 1.366245e-50 | -8.615850e-47 |
| Msa0588040 | Msa1208830 | 0.823837 | 1.117030e-53 | -8.615850e-47 |
| Msa0589720 | Msa1208830 | 0.809863 | 1.533303e-50 | -8.615850e-47 |
| Msa0589730 | Msa1208830 | 0.811794 | 5.857459e-51 | -8.615850e-47 |
| Msa0591490 | Msa1208830 | 0.807253 | 5.531739e-50 | -8.615850e-47 |
| Msa0594320 | Msa1208830 | 0.806650 | 7.420694e-50 | -8.615850e-47 |
| Msa0594330 | Msa1208830 | 0.833363 | 5.566397e-56 | -8.615850e-47 |
| Msa0597800 | Msa1208830 | 0.821788 | 3.351499e-53 | -8.615850e-47 |
| Msa0597820 | Msa1208830 | 0.850818 | 1.327574e-60 | -8.615850e-47 |
| Msa0854840 | Msa1208830 | 0.801936 | 7.117420e-49 | -8.615850e-47 |
| Msa0855260 | Msa1208830 | 0.807153 | 5.807436e-50 | -8.615850e-47 |
| Msa0865830 | Msa1208830 | 0.820122 | 8.105386e-53 | -8.615850e-47 |
| Msa0868870 | Msa1208830 | 0.822624 | 2.144248e-53 | -8.615850e-47 |
| Msa0874750 | Msa1208830 | 0.821006 | 5.077958e-53 | -8.615850e-47 |
| Msa0886370 | Msa1208830 | 0.808199 | 3.483478e-50 | -8.615850e-47 |
| Msa0896920 | Msa1208830 | 0.834251 | 3.338643e-56 | -8.615850e-47 |
| Msa0910590 | Msa1208830 | 0.813948 | 1.976312e-51 | -8.615850e-47 |
| Msa0911490 | Msa1208830 | -0.805026 | 1.628023e-49 | -8.615850e-47 |
| Msa0913500 | Msa1208830 | 0.803171 | 3.959660e-49 | -8.615850e-47 |
| Msa0932900 | Msa1208830 | 0.818382 | 2.018041e-52 | -8.615850e-47 |
| Msa0932910 | Msa1208830 | 0.803168 | 3.964553e-49 | -8.615850e-47 |
| Msa0932930 | Msa1208830 | 0.819473 | 1.140069e-52 | -8.615850e-47 |
| Msa0948430 | Msa1208830 | 0.815510 | 8.910700e-52 | -8.615850e-47 |
| Msa0951060 | Msa1208830 | 0.816769 | 4.662275e-52 | -8.615850e-47 |
| Msa0970790 | Msa1208830 | 0.807677 | 4.498225e-50 | -8.615850e-47 |
| Msa0976400 | Msa1208830 | 0.824187 | 9.243986e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1208830 | MtrunA17_Chr4g0058331 | 95.146 | 206 | 10 | 0 | 1 | 206 | 1 | 206 | 8.45e-150 | 414 |
| Msa1208830 | MtrunA17_Chr4g0058311 | 95.631 | 206 | 9 | 0 | 1 | 206 | 1 | 206 | 2.82e-141 | 392 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1208830 | AT5G50200.1 | 51.196 | 209 | 91 | 5 | 1 | 202 | 1 | 205 | 8.64e-65 | 199 |
| Msa1208830 | AT5G50200.3 | 51.196 | 209 | 91 | 5 | 1 | 202 | 1 | 205 | 8.64e-65 | 199 |
| Msa1208830 | AT5G50200.2 | 51.196 | 209 | 91 | 5 | 1 | 202 | 1 | 205 | 8.64e-65 | 199 |
| Msa1208830 | AT4G24715.1 | 49.275 | 207 | 97 | 5 | 1 | 202 | 23 | 226 | 7.23e-63 | 195 |
Find 52 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAGCATAACTTCAGCTTTA+TGG | 0.105787 | 8_1:+67142000 | None:intergenic |
| CATTGTCTTCAGTGCTTTCT+CGG | 0.283646 | 8_1:-67141678 | Msa1208830:CDS |
| ATCTATGCAGTTATGCTATC+TGG | 0.299171 | 8_1:-67142094 | Msa1208830:intron |
| CTCAAATTCAATCACTGCTT+TGG | 0.355774 | 8_1:+67141605 | None:intergenic |
| CGCATTTGATTCCAATGATA+AGG | 0.357587 | 8_1:-67141798 | Msa1208830:CDS |
| TACTTACCTTGTCCATGCTT+AGG | 0.361592 | 8_1:+67142216 | None:intergenic |
| ACTTACCTTGTCCATGCTTA+GGG | 0.362581 | 8_1:+67142217 | None:intergenic |
| TTTGAGATCAATGCAATTAG+TGG | 0.365486 | 8_1:-67141728 | Msa1208830:CDS |
| TCTTACCCGTGTTCGTGGTT+TGG | 0.369045 | 8_1:+67141762 | None:intergenic |
| ATAAGCCACTTCCTTATCAT+TGG | 0.373409 | 8_1:+67141787 | None:intergenic |
| GAATCAACCACTCGAAGGTT+TGG | 0.410270 | 8_1:+67141861 | None:intergenic |
| TGTTTAGCTGAAATTTGTTA+TGG | 0.421382 | 8_1:-67142288 | Msa1208830:CDS |
| AATGATAAGGAAGTGGCTTA+TGG | 0.425454 | 8_1:-67141785 | Msa1208830:CDS |
| GCTTTCTCGGTTGTGTCCCT+AGG | 0.446758 | 8_1:-67141665 | Msa1208830:CDS |
| GTCCCTGGAGAGTTCATCCT+CGG | 0.457081 | 8_1:+67141940 | None:intergenic |
| TAAGAAGAGTCTGTTCCAGC+TGG | 0.469266 | 8_1:+67142028 | None:intergenic |
| GCTACTCTTACCCGTGTTCG+TGG | 0.490262 | 8_1:+67141757 | None:intergenic |
| AGCTGAAATTTGTTATGGAA+AGG | 0.496497 | 8_1:-67142283 | Msa1208830:CDS |
| CTTCTATATTGAAAAGAGGA+AGG | 0.500127 | 8_1:-67141636 | Msa1208830:CDS |
| AGACCGAGGATGAACTCTCC+AGG | 0.511838 | 8_1:-67141943 | Msa1208830:CDS |
| TGATTCCAATGATAAGGAAG+TGG | 0.518875 | 8_1:-67141792 | Msa1208830:CDS |
| TGTTCTTCTATATTGAAAAG+AGG | 0.526365 | 8_1:-67141640 | Msa1208830:CDS |
| GTTGGAAGCATTGTAGGGCA+TGG | 0.526614 | 8_1:+67141892 | None:intergenic |
| TAGGGGAAGCTGTGACCTCA+AGG | 0.526901 | 8_1:+67142235 | None:intergenic |
| CAATATAGAAGAACACGCCT+AGG | 0.537687 | 8_1:+67141648 | None:intergenic |
| CTACAATGCTTCCAACAAAA+CGG | 0.547064 | 8_1:-67141885 | Msa1208830:CDS |
| TAGACAAAATCAGTGGAACA+TGG | 0.559795 | 8_1:-67142069 | Msa1208830:CDS |
| TTCTATATTGAAAAGAGGAA+GGG | 0.562873 | 8_1:-67141635 | Msa1208830:CDS |
| CGAGAAAGCACTGAAGACAA+TGG | 0.583492 | 8_1:+67141679 | None:intergenic |
| GTCACAGCTTCCCCTAAGCA+TGG | 0.584742 | 8_1:-67142228 | Msa1208830:CDS |
| CTAAACAAGACTTTCCCAGC+TGG | 0.586305 | 8_1:-67142043 | Msa1208830:CDS |
| TATGGCCAAACCACGAACAC+GGG | 0.596066 | 8_1:-67141767 | Msa1208830:CDS |
| TTATGGCCAAACCACGAACA+CGG | 0.597854 | 8_1:-67141768 | Msa1208830:CDS |
| CTTACCTTGTCCATGCTTAG+GGG | 0.615805 | 8_1:+67142218 | None:intergenic |
| AGACAAAATCAGTGGAACAT+GGG | 0.618150 | 8_1:-67142068 | Msa1208830:CDS |
| TATGGCAGCACACAAGCTTG+TGG | 0.620979 | 8_1:-67142331 | Msa1208830:CDS |
| TTCTTTGAAAAGAACCCTTG+AGG | 0.622622 | 8_1:-67142250 | Msa1208830:CDS |
| AAGAAGAGTCTGTTCCAGCT+GGG | 0.623941 | 8_1:+67142029 | None:intergenic |
| GACCGAGGATGAACTCTCCA+GGG | 0.630791 | 8_1:-67141942 | Msa1208830:CDS |
| AGGGGAAGCTGTGACCTCAA+GGG | 0.635053 | 8_1:+67142236 | None:intergenic |
| AATATAGAAGAACACGCCTA+GGG | 0.636139 | 8_1:+67141649 | None:intergenic |
| ATCATTGGAATCAAATGCGT+AGG | 0.647510 | 8_1:+67141802 | None:intergenic |
| CCTCGGTCTTTCTCCAAGCG+CGG | 0.651070 | 8_1:+67141957 | None:intergenic |
| GCGCAGAATCAACCACTCGA+AGG | 0.651463 | 8_1:+67141856 | None:intergenic |
| TCTGGAGTAGACAAAATCAG+TGG | 0.658257 | 8_1:-67142076 | Msa1208830:CDS |
| GCTTCCCCTAAGCATGGACA+AGG | 0.668529 | 8_1:-67142222 | Msa1208830:intron |
| AAACGGTCCAAACCTTCGAG+TGG | 0.693663 | 8_1:-67141868 | Msa1208830:CDS |
| AGCAGATATCAAGAGTCGCG+TGG | 0.697710 | 8_1:+67141702 | None:intergenic |
| AGATATCAAGAGTCGCGTGG+CGG | 0.700580 | 8_1:+67141705 | None:intergenic |
| CCGCGCTTGGAGAAAGACCG+AGG | 0.703551 | 8_1:-67141957 | Msa1208830:CDS |
| AAAGCTGAAGTTATGCTACG+CGG | 0.775950 | 8_1:-67141996 | Msa1208830:CDS |
| GTAGGCACGTACAAAGTACG+TGG | 0.784284 | 8_1:+67141820 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAAATTGAGTTTATATTGA+AGG | + | chr8_1:67141823-67141842 | None:intergenic | 20.0% |
| !!! | TGTTTAGCTGAAATTTGTTA+TGG | - | chr8_1:67141658-67141677 | Msa1208830:CDS | 25.0% |
| ! | TAATTAAATACGCCAATACA+AGG | + | chr8_1:67141772-67141791 | None:intergenic | 25.0% |
| ! | TGTTCTTCTATATTGAAAAG+AGG | - | chr8_1:67142306-67142325 | Msa1208830:CDS | 25.0% |
| ! | TTCTATATTGAAAAGAGGAA+GGG | - | chr8_1:67142311-67142330 | Msa1208830:CDS | 25.0% |
| !! | AGCTGAAATTTGTTATGGAA+AGG | - | chr8_1:67141663-67141682 | Msa1208830:CDS | 30.0% |
| ! | TAAGATTTTGCACCTTGTAT+TGG | - | chr8_1:67141757-67141776 | Msa1208830:CDS | 30.0% |
| ! | TTTGAGATCAATGCAATTAG+TGG | - | chr8_1:67142218-67142237 | Msa1208830:intron | 30.0% |
| CTTCTATATTGAAAAGAGGA+AGG | - | chr8_1:67142310-67142329 | Msa1208830:CDS | 30.0% | |
| ! | TTCTTTGAAAAGAACCCTTG+AGG | - | chr8_1:67141696-67141715 | Msa1208830:CDS | 35.0% |
| ATCTATGCAGTTATGCTATC+TGG | - | chr8_1:67141852-67141871 | Msa1208830:CDS | 35.0% | |
| TAGACAAAATCAGTGGAACA+TGG | - | chr8_1:67141877-67141896 | Msa1208830:CDS | 35.0% | |
| AGACAAAATCAGTGGAACAT+GGG | - | chr8_1:67141878-67141897 | Msa1208830:CDS | 35.0% | |
| GTAGCATAACTTCAGCTTTA+TGG | + | chr8_1:67141949-67141968 | None:intergenic | 35.0% | |
| !!! | GTTTTGTTGGAAGCATTGTA+GGG | + | chr8_1:67142062-67142081 | None:intergenic | 35.0% |
| CTACAATGCTTCCAACAAAA+CGG | - | chr8_1:67142061-67142080 | Msa1208830:CDS | 35.0% | |
| ATCATTGGAATCAAATGCGT+AGG | + | chr8_1:67142147-67142166 | None:intergenic | 35.0% | |
| CGCATTTGATTCCAATGATA+AGG | - | chr8_1:67142148-67142167 | Msa1208830:intron | 35.0% | |
| TGATTCCAATGATAAGGAAG+TGG | - | chr8_1:67142154-67142173 | Msa1208830:intron | 35.0% | |
| ATAAGCCACTTCCTTATCAT+TGG | + | chr8_1:67142162-67142181 | None:intergenic | 35.0% | |
| AATGATAAGGAAGTGGCTTA+TGG | - | chr8_1:67142161-67142180 | Msa1208830:intron | 35.0% | |
| AATATAGAAGAACACGCCTA+GGG | + | chr8_1:67142300-67142319 | None:intergenic | 35.0% | |
| ACTTACCTTGTCCATGCTTA+GGG | + | chr8_1:67141732-67141751 | None:intergenic | 40.0% | |
| TACTTACCTTGTCCATGCTT+AGG | + | chr8_1:67141733-67141752 | None:intergenic | 40.0% | |
| ! | TCTGGAGTAGACAAAATCAG+TGG | - | chr8_1:67141870-67141889 | Msa1208830:CDS | 40.0% |
| AAAGCTGAAGTTATGCTACG+CGG | - | chr8_1:67141950-67141969 | Msa1208830:CDS | 40.0% | |
| !!! | CGTTTTGTTGGAAGCATTGT+AGG | + | chr8_1:67142063-67142082 | None:intergenic | 40.0% |
| CATTGTCTTCAGTGCTTTCT+CGG | - | chr8_1:67142268-67142287 | Msa1208830:CDS | 40.0% | |
| CAATATAGAAGAACACGCCT+AGG | + | chr8_1:67142301-67142320 | None:intergenic | 40.0% | |
| CTTACCTTGTCCATGCTTAG+GGG | + | chr8_1:67141731-67141750 | None:intergenic | 45.0% | |
| CTAAACAAGACTTTCCCAGC+TGG | - | chr8_1:67141903-67141922 | Msa1208830:CDS | 45.0% | |
| AAGAAGAGTCTGTTCCAGCT+GGG | + | chr8_1:67141920-67141939 | None:intergenic | 45.0% | |
| TAAGAAGAGTCTGTTCCAGC+TGG | + | chr8_1:67141921-67141940 | None:intergenic | 45.0% | |
| !! | GTGTTGACAAGTTTTGTCCC+TGG | + | chr8_1:67142024-67142043 | None:intergenic | 45.0% |
| ! | GAAGGTTTGGACCGTTTTGT+TGG | + | chr8_1:67142075-67142094 | None:intergenic | 45.0% |
| GAATCAACCACTCGAAGGTT+TGG | + | chr8_1:67142088-67142107 | None:intergenic | 45.0% | |
| TTATGGCCAAACCACGAACA+CGG | - | chr8_1:67142178-67142197 | Msa1208830:intron | 45.0% | |
| !! | CGAGAAAGCACTGAAGACAA+TGG | + | chr8_1:67142270-67142289 | None:intergenic | 45.0% |
| !!! | AGCGCGGTCTTTTTGGCTTA+TGG | + | chr8_1:67141976-67141995 | None:intergenic | 50.0% |
| TAAGCCAAAAAGACCGCGCT+TGG | - | chr8_1:67141976-67141995 | Msa1208830:CDS | 50.0% | |
| ! | TTCTCCAAGCGCGGTCTTTT+TGG | + | chr8_1:67141983-67142002 | None:intergenic | 50.0% |
| !! | GTTGGAAGCATTGTAGGGCA+TGG | + | chr8_1:67142057-67142076 | None:intergenic | 50.0% |
| AAACGGTCCAAACCTTCGAG+TGG | - | chr8_1:67142078-67142097 | Msa1208830:CDS | 50.0% | |
| GTAGGCACGTACAAAGTACG+TGG | + | chr8_1:67142129-67142148 | None:intergenic | 50.0% | |
| TATGGCCAAACCACGAACAC+GGG | - | chr8_1:67142179-67142198 | Msa1208830:intron | 50.0% | |
| TCTTACCCGTGTTCGTGGTT+TGG | + | chr8_1:67142187-67142206 | None:intergenic | 50.0% | |
| AGATATCAAGAGTCGCGTGG+CGG | + | chr8_1:67142244-67142263 | None:intergenic | 50.0% | |
| AGCAGATATCAAGAGTCGCG+TGG | + | chr8_1:67142247-67142266 | None:intergenic | 50.0% | |
| AGGGGAAGCTGTGACCTCAA+GGG | + | chr8_1:67141713-67141732 | None:intergenic | 55.0% | |
| TAGGGGAAGCTGTGACCTCA+AGG | + | chr8_1:67141714-67141733 | None:intergenic | 55.0% | |
| GTCACAGCTTCCCCTAAGCA+TGG | - | chr8_1:67141718-67141737 | Msa1208830:CDS | 55.0% | |
| GCTTCCCCTAAGCATGGACA+AGG | - | chr8_1:67141724-67141743 | Msa1208830:CDS | 55.0% | |
| AGACCGAGGATGAACTCTCC+AGG | - | chr8_1:67142003-67142022 | Msa1208830:CDS | 55.0% | |
| GACCGAGGATGAACTCTCCA+GGG | - | chr8_1:67142004-67142023 | Msa1208830:CDS | 55.0% | |
| GTCCCTGGAGAGTTCATCCT+CGG | + | chr8_1:67142009-67142028 | None:intergenic | 55.0% | |
| GCGCAGAATCAACCACTCGA+AGG | + | chr8_1:67142093-67142112 | None:intergenic | 55.0% | |
| CGTACAAAGTACGTGGCCTG+AGG | + | chr8_1:67142122-67142141 | None:intergenic | 55.0% | |
| GCTACTCTTACCCGTGTTCG+TGG | + | chr8_1:67142192-67142211 | None:intergenic | 55.0% | |
| GCTTTCTCGGTTGTGTCCCT+AGG | - | chr8_1:67142281-67142300 | Msa1208830:CDS | 55.0% | |
| CCTCGGTCTTTCTCCAAGCG+CGG | + | chr8_1:67141992-67142011 | None:intergenic | 60.0% | |
| CCGCGCTTGGAGAAAGACCG+AGG | - | chr8_1:67141989-67142008 | Msa1208830:CDS | 65.0% | |
| GATTCTGCGCGACGTGCCTC+AGG | - | chr8_1:67142103-67142122 | Msa1208830:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 67141616 | 67142352 | 67141616 | ID=Msa1208830;Name=Msa1208830 |
| chr8_1 | mRNA | 67141616 | 67142352 | 67141616 | ID=Msa1208830-mRNA-1;Parent=Msa1208830;Name=Msa1208830-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|1|0|1|1|1|2|0|206 |
| chr8_1 | exon | 67142223 | 67142352 | 67142223 | ID=Msa1208830-mRNA-1:exon:22130;Parent=Msa1208830-mRNA-1 |
| chr8_1 | exon | 67141616 | 67142106 | 67141616 | ID=Msa1208830-mRNA-1:exon:22129;Parent=Msa1208830-mRNA-1 |
| chr8_1 | CDS | 67142223 | 67142352 | 67142223 | ID=Msa1208830-mRNA-1:cds;Parent=Msa1208830-mRNA-1 |
| chr8_1 | CDS | 67141616 | 67142106 | 67141616 | ID=Msa1208830-mRNA-1:cds;Parent=Msa1208830-mRNA-1 |
| Gene Sequence |
| Protein sequence |
