AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0549110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0549110 MtrunA17_Chr4g0033261 91.903 494 23 1 1 477 1 494 0.0 942
Msa0549110 MtrunA17_Chr4g0033231 86.134 476 49 1 19 477 19 494 0.0 855
Msa0549110 MtrunA17_Chr2g0329201 66.004 503 125 4 17 476 8 507 0.0 675
Msa0549110 MtrunA17_Chr2g0329191 64.155 491 128 5 29 476 24 509 0.0 647
Msa0549110 MtrunA17_Chr6g0482071 63.035 514 139 6 9 477 7 514 0.0 641
Msa0549110 MtrunA17_Chr4g0006111 65.768 482 134 7 23 476 47 525 0.0 634
Msa0549110 MtrunA17_Chr6g0482131 61.829 503 147 4 18 477 13 513 0.0 630
Msa0549110 MtrunA17_Chr4g0033271 60.672 506 152 4 14 476 10 511 0.0 615
Msa0549110 MtrunA17_Chr6g0482081 61.431 503 150 4 18 477 13 514 0.0 611
Msa0549110 MtrunA17_Chr3g0088441 57.885 520 171 4 1 477 1 515 0.0 611
Msa0549110 MtrunA17_Chr4g0033201 58.893 506 160 4 17 476 9 512 0.0 609
Msa0549110 MtrunA17_Chr4g0033341 59.082 501 162 4 17 476 45 543 0.0 608
Msa0549110 MtrunA17_Chr4g0033311 57.308 520 175 4 1 477 1 516 0.0 605
Msa0549110 MtrunA17_Chr4g0033401 59.961 507 152 4 17 474 4 508 0.0 603
Msa0549110 MtrunA17_Chr2g0329321 60.852 493 120 5 27 476 26 488 0.0 600
Msa0549110 MtrunA17_Chr4g0033191 59.109 494 154 4 29 476 28 519 0.0 600
Msa0549110 MtrunA17_Chr4g0033321 59.136 509 158 4 17 477 4 510 0.0 599
Msa0549110 MtrunA17_Chr4g0033331 59.557 497 156 3 21 474 19 513 0.0 598
Msa0549110 MtrunA17_Chr4g0006131 63.505 485 147 9 18 475 19 500 0.0 594
Msa0549110 MtrunA17_Chr4g0010501 55.625 480 164 4 39 476 22 494 0.0 541
Msa0549110 MtrunA17_Chr5g0430451 54.808 520 147 6 17 476 7 498 0.0 540
Msa0549110 MtrunA17_Chr2g0301231 66.244 394 105 7 109 476 1 392 0.0 511
Msa0549110 MtrunA17_Chr3g0087261 49.064 481 196 5 39 476 35 509 9.59e-165 475
Msa0549110 MtrunA17_Chr3g0087271 47.461 512 220 5 7 476 6 510 1.65e-162 469
Msa0549110 MtrunA17_Chr4g0043091 47.327 505 206 8 23 475 6 502 1.72e-157 456
Msa0549110 MtrunA17_Chr4g0042171 46.804 485 211 5 35 475 18 499 2.05e-149 436
Msa0549110 MtrunA17_Chr3g0090131 46.809 470 210 7 40 475 20 483 7.52e-149 434
Msa0549110 MtrunA17_Chr3g0090111 46.695 469 209 8 41 475 19 480 2.18e-148 432
Msa0549110 MtrunA17_Chr6g0482941 41.923 520 251 8 1 477 2 513 1.86e-142 418
Msa0549110 MtrunA17_Chr6g0482951 42.442 516 242 9 6 477 9 513 2.60e-141 415
Msa0549110 MtrunA17_Chr4g0074561 43.340 473 222 5 46 474 40 510 4.96e-134 397
Msa0549110 MtrunA17_Chr2g0288071 42.801 507 235 8 18 477 45 543 6.64e-134 397
Msa0549110 MtrunA17_Chr4g0074551 44.676 479 210 10 46 477 55 525 1.72e-127 382
Msa0549110 MtrunA17_Chr2g0317841 42.348 477 229 7 40 474 31 503 4.02e-126 377
Msa0549110 MtrunA17_Chr2g0317821 42.292 480 223 8 41 474 32 503 3.00e-125 375
Msa0549110 MtrunA17_Chr2g0288021 38.976 508 253 9 17 477 13 510 1.34e-118 357
Msa0549110 MtrunA17_Chr7g0242691 39.960 498 239 9 25 477 14 496 2.60e-118 357
Msa0549110 MtrunA17_Chr4g0006971 40.452 487 221 10 40 477 26 492 7.37e-113 342
Msa0549110 MtrunA17_Chr3g0087201 52.978 319 115 4 185 476 34 344 6.66e-112 334
Msa0549110 MtrunA17_Chr7g0242681 39.834 482 228 10 41 477 38 502 1.05e-111 340
Msa0549110 MtrunA17_Chr7g0234091 40.466 472 230 7 42 476 76 533 2.26e-107 329
Msa0549110 MtrunA17_Chr5g0428831 38.797 482 227 9 42 475 28 489 2.62e-107 328
Msa0549110 MtrunA17_Chr1g0195611 39.167 480 227 9 42 475 28 488 3.56e-106 325
Msa0549110 MtrunA17_Chr7g0234111 40.764 471 226 7 42 474 76 531 6.54e-105 323
Msa0549110 MtrunA17_Chr7g0234131 38.136 472 238 7 42 474 76 532 2.09e-100 311
Msa0549110 MtrunA17_Chr5g0418871 47.826 299 110 5 60 315 2 297 1.62e-89 276
Msa0549110 MtrunA17_Chr1g0195641 40.103 389 175 7 43 386 29 404 2.81e-86 270
Msa0549110 MtrunA17_Chr7g0230651 39.344 366 173 6 96 424 26 379 2.20e-81 257
Msa0549110 MtrunA17_Chr3g0087191 63.636 165 60 0 22 186 3 167 1.25e-73 229
Msa0549110 MtrunA17_Chr7g0227561 36.882 263 104 4 42 268 47 283 9.76e-52 177
Msa0549110 MtrunA17_Chr7g0236731 40.756 238 97 4 130 323 28 265 9.19e-43 153
Msa0549110 MtrunA17_Chr5g0418861 52.222 90 42 1 386 474 1 90 7.06e-26 100
Msa0549110 MtrunA17_Chr4g0068871 26.010 396 213 13 105 470 183 528 2.78e-21 97.1
Msa0549110 MtrunA17_Chr8g0352881 43.299 97 55 0 225 321 58 154 5.33e-20 87.0
Msa0549110 MtrunA17_Chr8g0352881 51.852 54 25 1 50 103 7 59 3.09e-12 64.7
Msa0549110 MtrunA17_Chr2g0301221 70.175 57 16 1 26 81 49 105 1.62e-14 69.7
Msa0549110 MtrunA17_Chr7g0227591 51.923 52 25 0 423 474 14 65 3.87e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0549110 AT2G44450.1 50.625 480 187 5 41 477 31 503 1.48e-170 490
Msa0549110 AT5G44640.1 48.141 511 210 7 11 477 5 504 5.38e-169 486
Msa0549110 AT2G44480.1 48.975 488 205 3 29 474 23 508 2.87e-168 484
Msa0549110 AT2G44480.3 48.975 488 205 3 29 474 54 539 1.00e-167 484
Msa0549110 AT5G42260.1 48.814 506 202 8 10 470 4 497 1.94e-167 482
Msa0549110 AT3G60130.1 50.000 482 190 6 38 475 27 501 2.87e-164 474
Msa0549110 AT5G24550.1 46.863 510 221 8 14 476 5 511 5.93e-155 451
Msa0549110 AT2G25630.1 46.850 508 197 8 11 474 5 483 9.43e-155 449
Msa0549110 AT1G26560.1 48.536 478 199 5 41 474 32 506 1.24e-153 447
Msa0549110 AT5G54570.1 46.584 483 212 4 36 474 25 505 2.29e-152 444
Msa0549110 AT5G24540.1 46.863 510 221 8 14 476 5 511 7.99e-149 436
Msa0549110 AT5G36890.1 47.010 485 212 7 25 475 7 480 1.29e-148 433
Msa0549110 AT5G36890.2 47.010 485 212 7 25 475 7 480 1.52e-148 433
Msa0549110 AT3G60120.1 47.541 488 200 10 38 476 11 491 5.62e-147 431
Msa0549110 AT3G18070.3 46.170 470 212 8 41 476 30 492 1.19e-146 429
Msa0549110 AT3G60140.1 43.555 512 230 8 11 476 6 504 4.98e-146 430
Msa0549110 AT2G44490.1 45.510 490 216 8 34 475 7 493 2.58e-145 427
Msa0549110 AT1G51470.1 46.122 477 208 6 40 476 44 511 4.67e-145 425
Msa0549110 AT1G47600.1 45.493 477 211 6 40 476 44 511 1.92e-144 424
Msa0549110 AT1G47600.2 45.493 477 210 6 40 476 44 510 2.96e-144 423
Msa0549110 AT3G18070.1 45.493 477 213 8 40 476 29 498 1.03e-143 421
Msa0549110 AT3G18080.1 45.303 479 213 8 40 476 38 509 6.73e-141 414
Msa0549110 AT2G44470.3 42.829 509 237 9 14 476 7 507 2.90e-140 416
Msa0549110 AT2G44460.1 43.836 511 232 10 10 474 3 504 6.17e-136 404
Msa0549110 AT2G44480.2 46.814 408 173 3 109 474 1 406 1.13e-134 395
Msa0549110 AT3G60130.2 48.921 417 162 6 103 475 40 449 1.15e-134 397
Msa0549110 AT1G66280.1 42.967 519 233 13 7 474 4 510 4.45e-130 387
Msa0549110 AT1G75940.1 44.559 487 211 10 41 474 37 517 5.10e-130 387
Msa0549110 AT3G03640.1 39.922 516 255 8 9 476 6 514 1.03e-129 387
Msa0549110 AT5G54570.3 44.988 429 191 3 36 421 25 451 1.73e-129 383
Msa0549110 AT3G09260.1 42.531 482 227 8 41 476 35 512 7.27e-129 384
Msa0549110 AT1G61820.1 41.016 512 249 9 9 476 4 506 1.21e-128 383
Msa0549110 AT3G21370.1 43.058 497 222 11 30 475 26 512 2.33e-128 383
Msa0549110 AT5G25980.2 41.243 531 248 14 1 475 1 523 9.59e-128 382
Msa0549110 AT1G52400.3 42.685 499 225 11 30 477 30 518 5.85e-127 379
Msa0549110 AT1G52400.1 42.685 499 225 11 30 477 30 518 5.85e-127 379
Msa0549110 AT1G66270.1 42.600 500 228 10 30 476 19 512 6.93e-127 379
Msa0549110 AT2G32860.2 40.909 484 238 6 40 477 93 574 6.44e-126 380
Msa0549110 AT2G32860.1 41.116 484 236 7 40 477 93 573 1.04e-125 379
Msa0549110 AT3G60130.3 47.482 417 157 7 103 475 40 438 1.65e-125 373
Msa0549110 AT5G26000.1 41.176 527 227 14 15 474 1 511 2.60e-125 375
Msa0549110 AT5G28510.1 41.365 498 231 10 30 474 30 519 3.50e-124 372
Msa0549110 AT1G66270.2 42.400 500 227 11 30 476 19 510 7.96e-124 371
Msa0549110 AT5G24540.2 45.475 453 198 7 14 420 5 454 4.15e-123 367
Msa0549110 AT1G61810.1 41.667 480 233 8 41 476 33 509 1.70e-119 360
Msa0549110 AT1G02850.2 40.984 488 218 10 36 475 21 486 2.60e-119 358
Msa0549110 AT2G44470.1 41.814 452 210 8 14 420 7 450 1.09e-117 353
Msa0549110 AT5G54570.2 44.878 410 180 4 109 474 1 408 1.40e-117 352
Msa0549110 AT1G02850.5 39.335 511 217 11 36 475 21 509 5.13e-115 348
Msa0549110 AT4G21760.2 39.208 505 254 8 15 474 25 521 2.08e-114 348
Msa0549110 AT1G61810.3 41.756 467 225 8 41 463 33 496 2.09e-114 348
Msa0549110 AT4G27830.1 39.442 502 236 10 18 474 6 484 2.64e-114 346
Msa0549110 AT3G60140.2 42.619 420 183 7 11 385 6 412 6.63e-114 343
Msa0549110 AT1G02850.4 39.258 512 217 12 36 475 21 510 3.91e-113 343
Msa0549110 AT2G44450.2 46.797 359 141 5 162 477 1 352 4.33e-112 335
Msa0549110 AT5G25980.3 40.842 475 219 12 1 421 1 467 6.67e-111 336
Msa0549110 AT5G25980.1 40.842 475 219 12 1 421 1 467 6.67e-111 336
Msa0549110 AT5G26000.2 40.678 472 199 12 15 421 1 456 1.62e-108 330
Msa0549110 AT4G27820.1 39.917 481 223 9 40 474 22 482 1.86e-108 331
Msa0549110 AT4G21760.1 38.416 505 248 9 15 474 25 511 1.89e-108 332
Msa0549110 AT3G62750.1 38.163 490 244 10 22 474 6 473 2.18e-107 328
Msa0549110 AT3G62750.2 38.163 490 244 10 22 474 32 499 3.73e-107 328
Msa0549110 AT1G02850.1 39.139 488 200 11 36 475 21 459 5.88e-107 326
Msa0549110 AT3G18070.2 40.046 437 218 8 40 476 29 421 1.85e-106 323
Msa0549110 AT4G22100.1 37.860 486 240 10 34 475 16 483 3.38e-105 323
Msa0549110 AT2G44480.4 50.161 311 113 2 29 297 54 364 2.41e-103 314
Msa0549110 AT3G62740.1 36.253 491 251 10 25 474 9 478 2.01e-102 315
Msa0549110 AT1G60090.2 35.833 480 247 8 41 477 24 485 7.54e-102 314
Msa0549110 AT1G52400.2 41.043 441 200 10 30 420 30 460 1.50e-101 312
Msa0549110 AT1G60090.1 34.774 486 251 9 41 477 24 492 4.26e-99 307
Msa0549110 AT1G45191.4 36.590 481 236 9 42 477 30 486 4.60e-99 307
Msa0549110 AT1G45191.6 36.590 481 247 8 42 477 30 497 1.84e-98 306
Msa0549110 AT1G45191.2 35.908 479 248 7 42 477 30 492 1.43e-97 303
Msa0549110 AT2G44470.2 40.967 393 179 8 14 361 7 391 2.01e-97 299
Msa0549110 AT5G48375.1 39.326 445 185 12 25 439 22 411 4.79e-97 300
Msa0549110 AT1G45191.1 36.270 488 251 9 42 477 30 509 7.00e-97 302
Msa0549110 AT2G44480.5 48.727 275 139 1 200 474 30 302 9.98e-95 289
Msa0549110 AT1G61810.5 40.049 412 200 8 109 476 1 409 9.46e-92 285
Msa0549110 AT3G18070.4 43.529 340 148 6 41 340 30 365 6.42e-91 282
Msa0549110 AT3G62750.4 37.130 439 218 9 21 423 31 447 1.38e-90 283
Msa0549110 AT1G61810.4 40.874 389 184 7 41 385 33 419 2.51e-90 282
Msa0549110 AT1G02850.3 39.048 420 170 9 36 385 21 424 4.23e-89 279
Msa0549110 AT3G18070.7 48.014 277 140 3 200 476 37 309 5.14e-89 275
Msa0549110 AT4G27830.2 39.173 411 190 8 109 474 1 396 2.34e-88 276
Msa0549110 AT3G62740.2 36.009 436 218 9 25 420 9 423 6.10e-87 273
Msa0549110 AT4G27820.2 38.592 412 192 8 109 474 1 397 1.30e-82 262
Msa0549110 AT4G27820.3 38.592 412 192 8 109 474 1 397 1.30e-82 262
Msa0549110 AT2G44460.2 42.659 361 158 7 160 474 1 358 2.82e-81 259
Msa0549110 AT3G62750.7 39.759 332 157 5 22 319 6 328 3.89e-81 256
Msa0549110 AT3G62750.8 39.759 332 157 5 22 319 6 328 3.89e-81 256
Msa0549110 AT1G60270.1 38.710 341 161 4 41 339 24 358 2.30e-80 254
Msa0549110 AT3G62750.3 39.759 332 157 5 22 319 32 354 2.61e-80 256
Msa0549110 AT1G51490.1 41.860 344 148 8 50 352 29 361 4.83e-80 255
Msa0549110 AT3G62750.6 36.973 403 202 8 109 474 1 388 1.53e-79 253
Msa0549110 AT3G62750.5 36.973 403 202 8 109 474 1 388 1.53e-79 253
Msa0549110 AT1G61820.3 38.525 366 179 7 155 476 4 367 4.73e-78 248
Msa0549110 AT1G45191.5 36.311 347 172 4 42 346 30 369 9.58e-73 236
Msa0549110 AT1G45191.3 35.912 362 181 5 42 359 30 384 2.34e-72 235
Msa0549110 AT3G18070.5 36.599 347 179 6 40 386 29 334 4.63e-71 229
Msa0549110 AT1G61810.2 42.751 269 111 4 41 267 33 300 2.85e-67 218
Msa0549110 AT3G18070.6 45.455 187 101 1 200 386 37 222 5.13e-54 181
Msa0549110 AT1G60270.2 36.630 273 129 3 109 339 1 271 6.70e-53 180
Msa0549110 AT5G16580.1 33.829 269 145 5 185 431 42 299 4.37e-41 149
Msa0549110 AT3G06510.1 25.369 406 215 17 104 475 159 510 5.52e-20 93.6
Msa0549110 AT3G06510.2 25.708 424 225 19 104 475 159 544 1.08e-19 92.8

Find 93 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGCAACTTACATAATATT+AGG 0.191120 4_1:+43907202 None:intergenic
GTGGAAGAGGACAAAGTATT+TGG 0.192835 4_1:-43909129 Msa0549110:CDS
TTGATTTACATAACAGAAAA+TGG 0.216843 4_1:-43905893 Msa0549110:intron
GAGCAGTCTAAGATGCTTAT+TGG 0.240397 4_1:-43906387 Msa0549110:CDS
TGGCATCAGGCTTCTCAAAA+TGG 0.265050 4_1:-43906727 Msa0549110:intron
TTATTACTTGGCAATATTCT+TGG 0.269887 4_1:+43907610 None:intergenic
GTAACAGCTGCTGCCATTAT+TGG 0.270052 4_1:+43909508 None:intergenic
TGGTATATTGGAAGCATAAT+TGG 0.270855 4_1:+43906329 None:intergenic
CGTGCTCTTGATTTCATGTT+TGG 0.298768 4_1:-43906628 Msa0549110:CDS
ACCAATGAGCTGAAAGCTTT+TGG 0.307343 4_1:+43905086 None:intergenic
ATTATTTGATTGTGGTATAT+TGG 0.312657 4_1:+43906317 None:intergenic
TAGCTCCTTTGTTCCAATAA+TGG 0.326020 4_1:-43909521 Msa0549110:CDS
GCCGGATACACCAGACGTTT+TGG 0.335510 4_1:-43905148 Msa0549110:CDS
ATCAATGAGCTACTTGATAA+TGG 0.365239 4_1:-43907422 Msa0549110:intron
CATCAATATTTCCTCTACTT+TGG 0.365921 4_1:-43909562 Msa0549110:CDS
TGAGCCACACTCTTACAGTT+TGG 0.367434 4_1:-43906906 Msa0549110:CDS
ACCAAAAGCTTTCAGCTCAT+TGG 0.373860 4_1:-43905087 Msa0549110:CDS
GCTTCTCAAAATGGTTCAAT+AGG 0.392323 4_1:-43906718 Msa0549110:CDS
TCCAAAACGTCTGGTGTATC+CGG 0.394369 4_1:+43905147 None:intergenic
ATGCAACTTACATAATATTA+GGG 0.399717 4_1:+43907203 None:intergenic
ACGAAAGACACCATGCTTGA+TGG 0.404375 4_1:+43906438 None:intergenic
CATGAAATCAAGAGCACGTT+CGG 0.407930 4_1:+43906633 None:intergenic
GTACTCGTCTTGTAGCGCTT+GGG 0.413064 4_1:+43907240 None:intergenic
GAGCCACACTCTTACAGTTT+GGG 0.414537 4_1:-43906905 Msa0549110:CDS
TGGAAGAGGACAAAGTATTT+GGG 0.414779 4_1:-43909128 Msa0549110:CDS
TCGGTGTTGCACCTGATGAT+TGG 0.418117 4_1:+43906814 None:intergenic
GGGGAAGATCCCAGTGGAAA+AGG 0.421057 4_1:+43907260 None:intergenic
TTACTCACCCTTGGTCCAAT+TGG 0.424891 4_1:+43906102 None:intergenic
TACAAAACGCACCTGGTAAG+AGG 0.428857 4_1:+43909408 None:intergenic
TGATCAGTATCATCGTTATA+AGG 0.429927 4_1:-43907898 Msa0549110:intron
TGTACTCGTCTTGTAGCGCT+TGG 0.432452 4_1:+43907239 None:intergenic
GACTGCTCTCTGGTGAACTT+TGG 0.446094 4_1:+43906402 None:intergenic
GAGCGTAACGGGATACCAAT+TGG 0.457796 4_1:-43906117 Msa0549110:CDS
TTTATGCAGCCACTGACAAC+AGG 0.464763 4_1:-43906468 Msa0549110:CDS
AGCAGTTCTTGAATCCCTCT+TGG 0.465436 4_1:+43905947 None:intergenic
CAAAGTAGAGGAAATATTGA+TGG 0.467715 4_1:+43909563 None:intergenic
CATACAGATTCTCTATCTCT+TGG 0.469897 4_1:-43907633 Msa0549110:CDS
AAAGTTAATTCCAAAACGTC+TGG 0.470833 4_1:+43905138 None:intergenic
TTTGGGTACGGAACCTTACA+TGG 0.476476 4_1:-43906888 Msa0549110:CDS
ATCACTTGAAGAGGCACTCA+TGG 0.486552 4_1:-43905411 Msa0549110:CDS
ATCAAGCATGGTGTCTTTCG+TGG 0.490440 4_1:-43906436 Msa0549110:CDS
GTACGGTCAACAAGTGTTGT+AGG 0.490892 4_1:+43909484 None:intergenic
TGGTTGTGTGAGGCCATGTA+AGG 0.493958 4_1:+43906875 None:intergenic
CACAAGGGCATGGCATTCAA+TGG 0.496087 4_1:-43909586 None:intergenic
GGCTATTTGTTTATCCAAGA+GGG 0.499794 4_1:-43905961 Msa0549110:CDS
TCGGAGACAGAGTGAAGCAT+TGG 0.500114 4_1:-43906940 Msa0549110:intron
GCATCACCTTAAATTGTCAC+TGG 0.506655 4_1:-43906696 Msa0549110:CDS
TACTGCCTCATCCTCTTACC+AGG 0.507287 4_1:-43909419 Msa0549110:intron
TGAGTATCCTTGAAATATCC+AGG 0.513235 4_1:+43906282 None:intergenic
GGGAAGATCCCAGTGGAAAA+GGG 0.516334 4_1:+43907261 None:intergenic
GTAAGAGTGTGGCTCATTGA+GGG 0.516603 4_1:+43906913 None:intergenic
CTCTTGATTTCATGTTTGGA+TGG 0.519547 4_1:-43906624 Msa0549110:intron
AAAATATTGGATAGAAGCAA+TGG 0.520670 4_1:-43907933 Msa0549110:CDS
ACGCACCTGGTAAGAGGATG+AGG 0.521862 4_1:+43909414 None:intergenic
AATAATGACACAAGCACACC+TGG 0.522457 4_1:-43906300 Msa0549110:CDS
GCTGCAAATGAAGGTGGAAG+AGG 0.523453 4_1:-43909142 Msa0549110:CDS
AAGCATCTTAGACTGCTCTC+TGG 0.526279 4_1:+43906392 None:intergenic
AAGCACACCTGGATATTTCA+AGG 0.530555 4_1:-43906289 Msa0549110:CDS
CAGCTGCATGAGCAAGGAGT+TGG 0.531565 4_1:+43906855 None:intergenic
ACTTTATAGAACCAATCATC+AGG 0.533812 4_1:-43906825 Msa0549110:intron
TGTAAGAGTGTGGCTCATTG+AGG 0.538006 4_1:+43906912 None:intergenic
TACTCGTCTTGTAGCGCTTG+GGG 0.539586 4_1:+43907241 None:intergenic
CAGTGGAAAAGGGTCACAAA+TGG 0.545800 4_1:+43907271 None:intergenic
CTTGTGTCATTATTTGATTG+TGG 0.555807 4_1:+43906309 None:intergenic
TGACAGCAGCTGCATGAGCA+AGG 0.559687 4_1:+43906849 None:intergenic
AAATATGCTCATGAACATCC+AGG 0.571960 4_1:-43909103 Msa0549110:intron
GTGTTGATAATGTAGTATGA+AGG 0.577599 4_1:-43909166 Msa0549110:intron
TGCTGCCATTATTGGAACAA+AGG 0.578034 4_1:+43909516 None:intergenic
TATGAAGGTGCTGCAAATGA+AGG 0.579768 4_1:-43909151 Msa0549110:CDS
GCAGAAGGAAAAGTAAGTGC+AGG 0.581251 4_1:-43907482 Msa0549110:intron
AGGCATGGTGTGAAAGTACA+AGG 0.584642 4_1:-43905211 Msa0549110:CDS
TATATTGGAAGCATAATTGG+AGG 0.589479 4_1:+43906332 None:intergenic
AACCATTAGGAAAACTACTA+CGG 0.590332 4_1:+43909452 None:intergenic
TCCAACGCTATCACTTGAAG+AGG 0.591945 4_1:-43905420 Msa0549110:CDS
TGGCTATTTGTTTATCCAAG+AGG 0.593511 4_1:-43905962 Msa0549110:CDS
GCCTCTTCAAGTGATAGCGT+TGG 0.596017 4_1:+43905419 None:intergenic
ACACTCTTACAGTTTGGGTA+CGG 0.597858 4_1:-43906900 Msa0549110:CDS
TCAAGCATGGTGTCTTTCGT+GGG 0.598348 4_1:-43906435 Msa0549110:CDS
GGATACTCTCCTGTTGTCAG+TGG 0.600363 4_1:+43906459 None:intergenic
CGGGATACCAATTGGACCAA+GGG 0.607534 4_1:-43906109 Msa0549110:intron
TTATGTAGGCTGCTTCACCT+TGG 0.615550 4_1:-43905982 Msa0549110:intron
AGTGCAGGTATAAATCAAGA+AGG 0.620837 4_1:-43907467 Msa0549110:CDS
AATTCAAGACACCAAAGTAG+AGG 0.621817 4_1:+43909551 None:intergenic
CTTGGATAAACAAATAGCCA+AGG 0.631378 4_1:+43905965 None:intergenic
TACTACGGTTTAGAGAAGTA+CGG 0.651396 4_1:+43909467 None:intergenic
AGGAGAGTATCCATCAAGCA+TGG 0.653040 4_1:-43906448 Msa0549110:CDS
GAAGGTGCTGCAAATGAAGG+TGG 0.659790 4_1:-43909148 Msa0549110:CDS
AGCAAGGAGTTGGTTGTGTG+AGG 0.661595 4_1:+43906865 None:intergenic
ACGGGATACCAATTGGACCA+AGG 0.664320 4_1:-43906110 Msa0549110:CDS
GTACCCAAACTGTAAGAGTG+TGG 0.677255 4_1:+43906902 None:intergenic
TCTAACGTGTCATTTGAGAA+CGG 0.686339 4_1:+43906667 None:intergenic
GCGCTACAAGACGAGTACAG+CGG 0.733888 4_1:-43907235 Msa0549110:CDS
GCGCTTGGGGAAGATCCCAG+TGG 0.736847 4_1:+43907254 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTGTTTGTTAAAAAAA+AGG - chr4_1:43905952-43905971 Msa0549110:CDS 10.0%
!!! ATTTTGTTTGTTAAAAAAAA+GGG - chr4_1:43905953-43905972 Msa0549110:CDS 10.0%
!! TCAAAAAAAAAAAAACTTAA+AGG + chr4_1:43905984-43906003 None:intergenic 10.0%
!!! AAAAAGCAAAAATAAATTTT+AGG - chr4_1:43906003-43906022 Msa0549110:intron 10.0%
!!! AAAATATTTTTCATCAAATT+AGG + chr4_1:43908479-43908498 None:intergenic 10.0%
!!! TTTTTTTTTTTTTTAAGAGA+TGG - chr4_1:43909011-43909030 Msa0549110:intron 10.0%
!!! TTTTTTTTTTTTTAAGAGAT+AGG - chr4_1:43909012-43909031 Msa0549110:intron 10.0%
!! AATATATCAGATATTAAAAT+AGG + chr4_1:43909383-43909402 None:intergenic 10.0%
!! AAATTGAGATAATTAAAGAA+AGG + chr4_1:43905584-43905603 None:intergenic 15.0%
!! TTTCTTTAATTATCTCAATT+TGG - chr4_1:43905583-43905602 Msa0549110:intron 15.0%
!!! CAAGTTATTTTATTTTTGAA+GGG + chr4_1:43905633-43905652 None:intergenic 15.0%
!!! TCAAGTTATTTTATTTTTGA+AGG + chr4_1:43905634-43905653 None:intergenic 15.0%
!!! ATAAGTGATATTTTATGTAT+TGG - chr4_1:43905723-43905742 Msa0549110:intron 15.0%
!! TCTTAACGAAAATTAATTAT+GGG + chr4_1:43906074-43906093 None:intergenic 15.0%
!! ATCTTAACGAAAATTAATTA+TGG + chr4_1:43906075-43906094 None:intergenic 15.0%
!! ACATGATTATATATCTTAAA+TGG - chr4_1:43906505-43906524 Msa0549110:intron 15.0%
!!! TATATTCCTTTAACTTTTTT+TGG + chr4_1:43906574-43906593 None:intergenic 15.0%
!!! AACCATTTTTACATTTTAAT+AGG - chr4_1:43906945-43906964 Msa0549110:intron 15.0%
!! TTCCTATTAAAATGTAAAAA+TGG + chr4_1:43906950-43906969 None:intergenic 15.0%
!!! TAAATAGGATTTTAAATTAC+AGG + chr4_1:43908095-43908114 None:intergenic 15.0%
!!! CAAATTGATTTTTTTATCAA+TGG + chr4_1:43908445-43908464 None:intergenic 15.0%
!!! ATATATCTAAAGTTAGTTAT+TGG - chr4_1:43908828-43908847 Msa0549110:intron 15.0%
!! TTAATAACTAAATTTAGGTA+TGG - chr4_1:43909181-43909200 Msa0549110:intron 15.0%
!!! ATTATTATTTCTGACTTTTT+TGG - chr4_1:43909400-43909419 Msa0549110:intron 15.0%
!!! AAACATACATTAGATGATTT+TGG + chr4_1:43905296-43905315 None:intergenic 20.0%
!! AATTGAAGCTTAAGAAATAT+TGG - chr4_1:43905422-43905441 Msa0549110:CDS 20.0%
!! AGAAAAAAAAGAAATATACC+TGG + chr4_1:43905555-43905574 None:intergenic 20.0%
!! CAAACAAAATATTATTGATC+AGG + chr4_1:43905943-43905962 None:intergenic 20.0%
!!! ACCTTAAATTTTCATACATT+AGG - chr4_1:43906384-43906403 Msa0549110:CDS 20.0%
!!! CATAACCTACATTTTAATTA+AGG - chr4_1:43906430-43906449 Msa0549110:CDS 20.0%
!! TCAAACCTTAATTAAAATGT+AGG + chr4_1:43906438-43906457 None:intergenic 20.0%
!!! ACATATATAGTTCTTTGTTT+GGG + chr4_1:43906547-43906566 None:intergenic 20.0%
!! AAACAAAGAACTATATATGT+GGG - chr4_1:43906547-43906566 Msa0549110:intron 20.0%
!! TTAATGTAGTAATTGCTTAT+TGG - chr4_1:43906664-43906683 Msa0549110:CDS 20.0%
!! ATCTCATACAGAAAAAATAT+TGG - chr4_1:43906691-43906710 Msa0549110:CDS 20.0%
!!! TGCTTTTATATCAATCAAAT+TGG + chr4_1:43906770-43906789 None:intergenic 20.0%
!!! AATTTTTTGTCTAGTTTATG+TGG - chr4_1:43906921-43906940 Msa0549110:CDS 20.0%
!!! ATTCAACATTTGTTATTACT+TGG + chr4_1:43907042-43907061 None:intergenic 20.0%
!! TATAGATAAAATGAAACTCT+AGG + chr4_1:43907088-43907107 None:intergenic 20.0%
!!! TAGAGTTTCATTTTATCTAT+AGG - chr4_1:43907087-43907106 Msa0549110:intron 20.0%
!! ATCATGGTAATATAAATTGA+TGG - chr4_1:43907321-43907340 Msa0549110:intron 20.0%
!! ATGCAACTTACATAATATTA+GGG + chr4_1:43907437-43907456 None:intergenic 20.0%
!! AAGCAATAATAATAAATCGA+TGG - chr4_1:43907474-43907493 Msa0549110:CDS 20.0%
!! GAAATTAACAAGTAAAACAT+CGG + chr4_1:43907623-43907642 None:intergenic 20.0%
!! AATCATAACAAATACAAGAA+AGG + chr4_1:43907860-43907879 None:intergenic 20.0%
!!! ATTGGTTCATTTGATTTTAT+TGG - chr4_1:43908268-43908287 Msa0549110:intron 20.0%
!! ATTATTTGATTGTGGTATAT+TGG + chr4_1:43908323-43908342 None:intergenic 20.0%
!!! TTTTCTGTTATGTAAATCAA+AGG + chr4_1:43908744-43908763 None:intergenic 20.0%
!! TTGATTTACATAACAGAAAA+TGG - chr4_1:43908744-43908763 Msa0549110:intron 20.0%
!! TGCATATTGTAAAAATCAAA+TGG + chr4_1:43908787-43908806 None:intergenic 20.0%
!!! ATTATACAATTGATTTGACT+AGG + chr4_1:43909150-43909169 None:intergenic 20.0%
!! TTTCCTAATGGTTTCATTTT+TGG - chr4_1:43905195-43905214 Msa0549110:CDS 25.0%
!!! TACTTTAAGACCAAAGATTT+AGG - chr4_1:43905382-43905401 Msa0549110:CDS 25.0%
!!! ACTTTAAGACCAAAGATTTA+GGG - chr4_1:43905383-43905402 Msa0549110:CDS 25.0%
!!! ATATTGGAGGTTAATTTGTA+AGG - chr4_1:43905438-43905457 Msa0549110:CDS 25.0%
! CAAAATAGAGTACATGAATA+CGG + chr4_1:43905827-43905846 None:intergenic 25.0%
! TTTAACCCTATTGTTATATG+TGG - chr4_1:43906146-43906165 Msa0549110:intron 25.0%
! GCCTAATGTATGAAAATTTA+AGG + chr4_1:43906388-43906407 None:intergenic 25.0%
! CACATATATAGTTCTTTGTT+TGG + chr4_1:43906548-43906567 None:intergenic 25.0%
! CAAACAAAGAACTATATATG+TGG - chr4_1:43906546-43906565 Msa0549110:intron 25.0%
!! TGAAAGTAGTCTTTTTACTT+TGG - chr4_1:43906632-43906651 Msa0549110:CDS 25.0%
! AAAATATTGGATAGAAGCAA+TGG - chr4_1:43906704-43906723 Msa0549110:CDS 25.0%
! CAAAATATTAGAGCTTCATA+AGG + chr4_1:43906896-43906915 None:intergenic 25.0%
! TTATTACTTGGCAATATTCT+TGG + chr4_1:43907030-43907049 None:intergenic 25.0%
! AAACTCTAGGTGAATTAAAA+TGG + chr4_1:43907075-43907094 None:intergenic 25.0%
! CTAAATGATGTTCATAATCA+TGG - chr4_1:43907305-43907324 Msa0549110:intron 25.0%
! ATGGTAATATAAATTGATGG+AGG - chr4_1:43907324-43907343 Msa0549110:intron 25.0%
! CATGCAACTTACATAATATT+AGG + chr4_1:43907438-43907457 None:intergenic 25.0%
! CTAATATTATGTAAGTTGCA+TGG - chr4_1:43907436-43907455 Msa0549110:CDS 25.0%
! ACAAATACAAGAAAGGTTTA+TGG + chr4_1:43907853-43907872 None:intergenic 25.0%
!! TCGAGTTAGTTAAAGTTTTT+CGG + chr4_1:43907885-43907904 None:intergenic 25.0%
! CTTTAACTAACTCGAATTAA+TGG - chr4_1:43907890-43907909 Msa0549110:intron 25.0%
! CCATAATCATGCATATAAAT+AGG + chr4_1:43908110-43908129 None:intergenic 25.0%
! CCTATTTATATGCATGATTA+TGG - chr4_1:43908107-43908126 Msa0549110:intron 25.0%
!!! TTCTTGAAAGACTTTTTTGT+AGG - chr4_1:43908146-43908165 Msa0549110:intron 25.0%
!!! ATGTTATTTCAATTCCTCAT+AGG + chr4_1:43908418-43908437 None:intergenic 25.0%
! TCAGTATACGATTGTATAAA+TGG + chr4_1:43908625-43908644 None:intergenic 25.0%
!! AATTTTCATTCTTAAGAGAG+AGG - chr4_1:43908978-43908997 Msa0549110:intron 25.0%
! CTCTCTTAAGAAAAAAGATT+AGG + chr4_1:43909040-43909059 None:intergenic 25.0%
!!! AATCTTTTTTCTTAAGAGAG+AGG - chr4_1:43909040-43909059 Msa0549110:intron 25.0%
! TATTGAGGGTAAAAAAGATT+AGG + chr4_1:43909103-43909122 None:intergenic 25.0%
! AGAATCAAACAATGTATTGA+GGG + chr4_1:43909117-43909136 None:intergenic 25.0%
! AAGAATCAAACAATGTATTG+AGG + chr4_1:43909118-43909137 None:intergenic 25.0%
! ATATGAAATAAGTAGAGAGA+TGG + chr4_1:43909320-43909339 None:intergenic 25.0%
! TAAAATAGGCAAACTAAGTA+AGG + chr4_1:43909369-43909388 None:intergenic 25.0%
!!! ATTTCTGACTTTTTTGGTTA+AGG - chr4_1:43909406-43909425 Msa0549110:intron 25.0%
CAAAGTAGAGGAAATATTGA+TGG + chr4_1:43905077-43905096 None:intergenic 30.0%
CATCAATATTTCCTCTACTT+TGG - chr4_1:43905075-43905094 Msa0549110:CDS 30.0%
AACCATTAGGAAAACTACTA+CGG + chr4_1:43905188-43905207 None:intergenic 30.0%
GTACCAAAAATGAAACCATT+AGG + chr4_1:43905201-43905220 None:intergenic 30.0%
! AAAATTTTACAAAACGCACC+TGG + chr4_1:43905239-43905258 None:intergenic 30.0%
TGAAGCTTAAGAAATATTGG+AGG - chr4_1:43905425-43905444 Msa0549110:CDS 30.0%
!! GTGTTGATAATGTAGTATGA+AGG - chr4_1:43905471-43905490 Msa0549110:intron 30.0%
! CAATTTGGTGAATAATAGCT+TGG - chr4_1:43905598-43905617 Msa0549110:intron 30.0%
GATAGTATGTAGCTTATAAG+TGG - chr4_1:43905684-43905703 Msa0549110:intron 30.0%
ATGTAGCTTATAAGTGGAAA+AGG - chr4_1:43905690-43905709 Msa0549110:intron 30.0%
TGTAGCTTATAAGTGGAAAA+GGG - chr4_1:43905691-43905710 Msa0549110:intron 30.0%
!! TTTATGTATTGGTCTTGTTC+TGG - chr4_1:43905734-43905753 Msa0549110:intron 30.0%
TGTAGTCATAATACTTATGC+TGG - chr4_1:43905872-43905891 Msa0549110:intron 30.0%
AATTGAACCAACTTGACAAA+CGG + chr4_1:43906117-43906136 None:intergenic 30.0%
AAACAAACTAATGAAGTCGT+CGG + chr4_1:43906176-43906195 None:intergenic 30.0%
AATATCGCATGAGAAAGAAT+AGG - chr4_1:43906348-43906367 Msa0549110:CDS 30.0%
CATGAGAAAGAATAGGAATT+GGG - chr4_1:43906355-43906374 Msa0549110:CDS 30.0%
ATGAGAAAGAATAGGAATTG+GGG - chr4_1:43906356-43906375 Msa0549110:CDS 30.0%
TAAGGTTTGAATTACAACTG+AGG - chr4_1:43906448-43906467 Msa0549110:CDS 30.0%
AAGGTTTGAATTACAACTGA+GGG - chr4_1:43906449-43906468 Msa0549110:CDS 30.0%
TGATCAGTATCATCGTTATA+AGG - chr4_1:43906739-43906758 Msa0549110:intron 30.0%
ATCAATGAGCTACTTGATAA+TGG - chr4_1:43907215-43907234 Msa0549110:CDS 30.0%
GATCAAATACTTGTGAAACA+TGG + chr4_1:43907255-43907274 None:intergenic 30.0%
! CATATGCAGAAAAGATTTTC+AGG - chr4_1:43907647-43907666 Msa0549110:CDS 30.0%
ACTTTATAGAACCAATCATC+AGG - chr4_1:43907812-43907831 Msa0549110:intron 30.0%
AAGAAAGGTTTATGGTTCTT+CGG + chr4_1:43907845-43907864 None:intergenic 30.0%
CAAAAACCAGTGACAATTTA+AGG + chr4_1:43907950-43907969 None:intergenic 30.0%
! TATATTGGAAGCATAATTGG+AGG + chr4_1:43908308-43908327 None:intergenic 30.0%
! TGGTATATTGGAAGCATAAT+TGG + chr4_1:43908311-43908330 None:intergenic 30.0%
! CTTGTGTCATTATTTGATTG+TGG + chr4_1:43908331-43908350 None:intergenic 30.0%
! TCTAAAGTTAGTTATTGGCT+TGG - chr4_1:43908833-43908852 Msa0549110:intron 30.0%
!! CTAAAGTTAGTTATTGGCTT+GGG - chr4_1:43908834-43908853 Msa0549110:intron 30.0%
! AAGTACAAGGATATTTTGCA+TGG - chr4_1:43909439-43909458 Msa0549110:CDS 30.0%
!! ATATTTTGCATGGTCACTTT+TGG - chr4_1:43909449-43909468 Msa0549110:CDS 30.0%
! CACTTTTGGACAATTTTGAA+TGG - chr4_1:43909463-43909482 Msa0549110:CDS 30.0%
AAAGTTAATTCCAAAACGTC+TGG + chr4_1:43909502-43909521 None:intergenic 30.0%
AATTCAAGACACCAAAGTAG+AGG + chr4_1:43905089-43905108 None:intergenic 35.0%
TAGCTCCTTTGTTCCAATAA+TGG - chr4_1:43905116-43905135 Msa0549110:CDS 35.0%
TACTACGGTTTAGAGAAGTA+CGG + chr4_1:43905173-43905192 None:intergenic 35.0%
! AACCGTAGTAGTTTTCCTAA+TGG - chr4_1:43905183-43905202 Msa0549110:CDS 35.0%
TAAGACCAAAGATTTAGGGA+TGG - chr4_1:43905387-43905406 Msa0549110:CDS 35.0%
TCATTCCATCCCTAAATCTT+TGG + chr4_1:43905395-43905414 None:intergenic 35.0%
! TGGAAGAGGACAAAGTATTT+GGG - chr4_1:43905509-43905528 Msa0549110:intron 35.0%
AAATATGCTCATGAACATCC+AGG - chr4_1:43905534-43905553 Msa0549110:intron 35.0%
AAACGTCATAGTTAAGCGAT+TGG + chr4_1:43906042-43906061 None:intergenic 35.0%
GACAAACGGAGTTAAGTTAT+GGG + chr4_1:43906103-43906122 None:intergenic 35.0%
TGACAAACGGAGTTAAGTTA+TGG + chr4_1:43906104-43906123 None:intergenic 35.0%
GTGGACCACATATAACAATA+GGG + chr4_1:43906154-43906173 None:intergenic 35.0%
GCATGAGAAAGAATAGGAAT+TGG - chr4_1:43906354-43906373 Msa0549110:CDS 35.0%
! TAATCATGTTTGAGACGTGT+CGG + chr4_1:43906494-43906513 None:intergenic 35.0%
GTGGGACCAAAAAAAGTTAA+AGG - chr4_1:43906565-43906584 Msa0549110:intron 35.0%
GACTACTTTCAGTAACAACA+TGG + chr4_1:43906623-43906642 None:intergenic 35.0%
CATACAGATTCTCTATCTCT+TGG - chr4_1:43907004-43907023 Msa0549110:intron 35.0%
AGTGCAGGTATAAATCAAGA+AGG - chr4_1:43907170-43907189 Msa0549110:intron 35.0%
!! ATTGATGGAGGTATTGATAC+AGG - chr4_1:43907336-43907355 Msa0549110:intron 35.0%
GACGTCATAAAGTTCTATCA+TGG + chr4_1:43907555-43907574 None:intergenic 35.0%
AAACCATATATCCTCATGTG+GGG + chr4_1:43907595-43907614 None:intergenic 35.0%
GAAACCATATATCCTCATGT+GGG + chr4_1:43907596-43907615 None:intergenic 35.0%
TGAAACCATATATCCTCATG+TGG + chr4_1:43907597-43907616 None:intergenic 35.0%
TAACTCGAATTAATGGCATC+AGG - chr4_1:43907897-43907916 Msa0549110:intron 35.0%
GCTTCTCAAAATGGTTCAAT+AGG - chr4_1:43907919-43907938 Msa0549110:CDS 35.0%
!! TCTAACGTGTCATTTGAGAA+CGG + chr4_1:43907973-43907992 None:intergenic 35.0%
CTCTTGATTTCATGTTTGGA+TGG - chr4_1:43908013-43908032 Msa0549110:intron 35.0%
TGAGTATCCTTGAAATATCC+AGG + chr4_1:43908358-43908377 None:intergenic 35.0%
TGCAGTTACTGCTATATATC+TGG - chr4_1:43908566-43908585 Msa0549110:intron 35.0%
GCAGTTACTGCTATATATCT+GGG - chr4_1:43908567-43908586 Msa0549110:intron 35.0%
CTTGGATAAACAAATAGCCA+AGG + chr4_1:43908675-43908694 None:intergenic 35.0%
TGGCTATTTGTTTATCCAAG+AGG - chr4_1:43908675-43908694 Msa0549110:intron 35.0%
GGCTATTTGTTTATCCAAGA+GGG - chr4_1:43908676-43908695 Msa0549110:intron 35.0%
!!! TGACTTTTTTGGTTAAGGCA+TGG - chr4_1:43909411-43909430 Msa0549110:intron 35.0%
TGCTGCCATTATTGGAACAA+AGG + chr4_1:43905124-43905143 None:intergenic 40.0%
!! TATGAAGGTGCTGCAAATGA+AGG - chr4_1:43905486-43905505 Msa0549110:intron 40.0%
! GTGGAAGAGGACAAAGTATT+TGG - chr4_1:43905508-43905527 Msa0549110:intron 40.0%
!! GTCTTGTTCTGGAAGTTAGT+AGG - chr4_1:43905745-43905764 Msa0549110:intron 40.0%
!! GTTCTGGAAGTTAGTAGGAA+AGG - chr4_1:43905750-43905769 Msa0549110:intron 40.0%
CTTAACTCCGTTTGTCAAGT+TGG - chr4_1:43906107-43906126 Msa0549110:intron 40.0%
GGTGGACCACATATAACAAT+AGG + chr4_1:43906155-43906174 None:intergenic 40.0%
TGCACTTAGGAGATGAGAAA+TGG + chr4_1:43906287-43906306 None:intergenic 40.0%
AATAGACAAGAGCTGCACTT+AGG + chr4_1:43906300-43906319 None:intergenic 40.0%
GTTTGAATTACAACTGAGGG+AGG - chr4_1:43906452-43906471 Msa0549110:CDS 40.0%
! AGCTCTAACAAACATGCAGA+AGG - chr4_1:43907140-43907159 Msa0549110:intron 40.0%
! ATTTGTGACCCTTTTCCACT+GGG - chr4_1:43907368-43907387 Msa0549110:intron 40.0%
AATGTAATTGACCCCACATG+AGG - chr4_1:43907581-43907600 Msa0549110:intron 40.0%
GAATTCCTTGAAGCATAGCT+CGG + chr4_1:43907679-43907698 None:intergenic 40.0%
GAGCTATGCTTCAAGGAATT+CGG - chr4_1:43907678-43907697 Msa0549110:intron 40.0%
ACACTCTTACAGTTTGGGTA+CGG - chr4_1:43907737-43907756 Msa0549110:intron 40.0%
GCATCACCTTAAATTGTCAC+TGG - chr4_1:43907941-43907960 Msa0549110:intron 40.0%
! CATGAAATCAAGAGCACGTT+CGG + chr4_1:43908007-43908026 None:intergenic 40.0%
CGTGCTCTTGATTTCATGTT+TGG - chr4_1:43908009-43908028 Msa0549110:intron 40.0%
CACAATGTTCCGCAAAATCA+TGG - chr4_1:43908052-43908071 Msa0549110:intron 40.0%
! TGTCGTGATCCATGATTTTG+CGG + chr4_1:43908064-43908083 None:intergenic 40.0%
! GAGCAGTCTAAGATGCTTAT+TGG - chr4_1:43908250-43908269 Msa0549110:intron 40.0%
AATAATGACACAAGCACACC+TGG - chr4_1:43908337-43908356 Msa0549110:intron 40.0%
AAGCACACCTGGATATTTCA+AGG - chr4_1:43908348-43908367 Msa0549110:intron 40.0%
AATAGAGTCTTACTCACCCT+TGG + chr4_1:43908547-43908566 None:intergenic 40.0%
CTGAGAAACGACACTTATGT+AGG - chr4_1:43908641-43908660 Msa0549110:intron 40.0%
!! TTAGTTATTGGCTTGGGATC+TGG - chr4_1:43908840-43908859 Msa0549110:intron 40.0%
!! TAGTTATTGGCTTGGGATCT+GGG - chr4_1:43908841-43908860 Msa0549110:intron 40.0%
! ATTCTCACCGGTGTCAATTT+AGG - chr4_1:43908894-43908913 Msa0549110:intron 40.0%
!! GACAATTTTGAATGGCTTGC+CGG - chr4_1:43909471-43909490 Msa0549110:CDS 40.0%
ACCAAAAGCTTTCAGCTCAT+TGG - chr4_1:43909550-43909569 Msa0549110:CDS 40.0%
! ACCAATGAGCTGAAAGCTTT+TGG + chr4_1:43909554-43909573 None:intergenic 40.0%
GTAACAGCTGCTGCCATTAT+TGG + chr4_1:43905132-43905151 None:intergenic 45.0%
! GTACGGTCAACAAGTGTTGT+AGG + chr4_1:43905156-43905175 None:intergenic 45.0%
TACAAAACGCACCTGGTAAG+AGG + chr4_1:43905232-43905251 None:intergenic 45.0%
CAAACTAATGAAGTCGTCGG+TGG + chr4_1:43906173-43906192 None:intergenic 45.0%
TTAGGCGACAAATACCTGTC+AGG - chr4_1:43906402-43906421 Msa0549110:CDS 45.0%
GCAGAAGGAAAAGTAAGTGC+AGG - chr4_1:43907155-43907174 Msa0549110:intron 45.0%
!! TGGAGGTATTGATACAGGTC+TGG - chr4_1:43907341-43907360 Msa0549110:intron 45.0%
CAGTGGAAAAGGGTCACAAA+TGG + chr4_1:43907369-43907388 None:intergenic 45.0%
! CATTTGTGACCCTTTTCCAC+TGG - chr4_1:43907367-43907386 Msa0549110:intron 45.0%
TGACCCCACATGAGGATATA+TGG - chr4_1:43907589-43907608 Msa0549110:intron 45.0%
! GTAAGAGTGTGGCTCATTGA+GGG + chr4_1:43907727-43907746 None:intergenic 45.0%
! TGTAAGAGTGTGGCTCATTG+AGG + chr4_1:43907728-43907747 None:intergenic 45.0%
TGAGCCACACTCTTACAGTT+TGG - chr4_1:43907731-43907750 Msa0549110:intron 45.0%
GAGCCACACTCTTACAGTTT+GGG - chr4_1:43907732-43907751 Msa0549110:intron 45.0%
GTACCCAAACTGTAAGAGTG+TGG + chr4_1:43907738-43907757 None:intergenic 45.0%
TTTGGGTACGGAACCTTACA+TGG - chr4_1:43907749-43907768 Msa0549110:intron 45.0%
TGGCATCAGGCTTCTCAAAA+TGG - chr4_1:43907910-43907929 Msa0549110:CDS 45.0%
TTTATGCAGCCACTGACAAC+AGG - chr4_1:43908169-43908188 Msa0549110:intron 45.0%
!! AGGAGAGTATCCATCAAGCA+TGG - chr4_1:43908189-43908208 Msa0549110:intron 45.0%
ACGAAAGACACCATGCTTGA+TGG + chr4_1:43908202-43908221 None:intergenic 45.0%
ATCAAGCATGGTGTCTTTCG+TGG - chr4_1:43908201-43908220 Msa0549110:intron 45.0%
TCAAGCATGGTGTCTTTCGT+GGG - chr4_1:43908202-43908221 Msa0549110:intron 45.0%
AAGCATCTTAGACTGCTCTC+TGG + chr4_1:43908248-43908267 None:intergenic 45.0%
CCTCATAGGAGTTGTGATCA+AGG + chr4_1:43908404-43908423 None:intergenic 45.0%
CCTTGATCACAACTCCTATG+AGG - chr4_1:43908401-43908420 Msa0549110:intron 45.0%
! CTTTTGACAGCTGAGCGTAA+CGG - chr4_1:43908508-43908527 Msa0549110:intron 45.0%
! TTTTGACAGCTGAGCGTAAC+GGG - chr4_1:43908509-43908528 Msa0549110:intron 45.0%
TTACTCACCCTTGGTCCAAT+TGG + chr4_1:43908538-43908557 None:intergenic 45.0%
TTATGTAGGCTGCTTCACCT+TGG - chr4_1:43908655-43908674 Msa0549110:intron 45.0%
AGCAGTTCTTGAATCCCTCT+TGG + chr4_1:43908693-43908712 None:intergenic 45.0%
! TCACCGGTGTCAATTTAGGT+GGG - chr4_1:43908898-43908917 Msa0549110:intron 45.0%
TCCAACGCTATCACTTGAAG+AGG - chr4_1:43909217-43909236 Msa0549110:intron 45.0%
!! ATCACTTGAAGAGGCACTCA+TGG - chr4_1:43909226-43909245 Msa0549110:intron 45.0%
! AGGCATGGTGTGAAAGTACA+AGG - chr4_1:43909426-43909445 Msa0549110:CDS 45.0%
TCCAAAACGTCTGGTGTATC+CGG + chr4_1:43909493-43909512 None:intergenic 45.0%
!! TTAAAAAAAAAAAAAAGATT+AGG + chr4_1:43909007-43909026 None:intergenic 5.0%
!! TTAAAAAAAAAAAAAAGATT+AGG + chr4_1:43909007-43909026 None:intergenic 5.0%
!!! AAATTTTAATAACTAAATTT+AGG - chr4_1:43909176-43909195 Msa0549110:intron 5.0%
TACTGCCTCATCCTCTTACC+AGG - chr4_1:43905218-43905237 Msa0549110:intron 50.0%
!! GAAGGTGCTGCAAATGAAGG+TGG - chr4_1:43905489-43905508 Msa0549110:intron 50.0%
GCTGCAAATGAAGGTGGAAG+AGG - chr4_1:43905495-43905514 Msa0549110:intron 50.0%
TAGGTTATGTAGCGCCTGAC+AGG + chr4_1:43906419-43906438 None:intergenic 50.0%
GGGAAGATCCCAGTGGAAAA+GGG + chr4_1:43907379-43907398 None:intergenic 50.0%
TACTCGTCTTGTAGCGCTTG+GGG + chr4_1:43907399-43907418 None:intergenic 50.0%
GTACTCGTCTTGTAGCGCTT+GGG + chr4_1:43907400-43907419 None:intergenic 50.0%
TGTACTCGTCTTGTAGCGCT+TGG + chr4_1:43907401-43907420 None:intergenic 50.0%
CTATGCCGAGCTATGCTTCA+AGG - chr4_1:43907671-43907690 Msa0549110:CDS 50.0%
TCGGAGACAGAGTGAAGCAT+TGG - chr4_1:43907697-43907716 Msa0549110:intron 50.0%
TGGTTGTGTGAGGCCATGTA+AGG + chr4_1:43907765-43907784 None:intergenic 50.0%
! AGCAAGGAGTTGGTTGTGTG+AGG + chr4_1:43907775-43907794 None:intergenic 50.0%
!! TCGGTGTTGCACCTGATGAT+TGG + chr4_1:43907826-43907845 None:intergenic 50.0%
GGATACTCTCCTGTTGTCAG+TGG + chr4_1:43908181-43908200 None:intergenic 50.0%
GACTGCTCTCTGGTGAACTT+TGG + chr4_1:43908238-43908257 None:intergenic 50.0%
! GAGCGTAACGGGATACCAAT+TGG - chr4_1:43908520-43908539 Msa0549110:intron 50.0%
ACGGGATACCAATTGGACCA+AGG - chr4_1:43908527-43908546 Msa0549110:intron 50.0%
CGGGATACCAATTGGACCAA+GGG - chr4_1:43908528-43908547 Msa0549110:intron 50.0%
GTCTCAGGTTCGATTCTCAC+CGG - chr4_1:43908882-43908901 Msa0549110:intron 50.0%
! CTCACCGGTGTCAATTTAGG+TGG - chr4_1:43908897-43908916 Msa0549110:intron 50.0%
! GCCTCTTCAAGTGATAGCGT+TGG + chr4_1:43909221-43909240 None:intergenic 50.0%
ACGCACCTGGTAAGAGGATG+AGG + chr4_1:43905226-43905245 None:intergenic 55.0%
GGGGAAGATCCCAGTGGAAA+AGG + chr4_1:43907380-43907399 None:intergenic 55.0%
GCGCTACAAGACGAGTACAG+CGG - chr4_1:43907402-43907421 Msa0549110:intron 55.0%
CAGCTGCATGAGCAAGGAGT+TGG + chr4_1:43907785-43907804 None:intergenic 55.0%
TGACAGCAGCTGCATGAGCA+AGG + chr4_1:43907791-43907810 None:intergenic 55.0%
GAATCGAACCTGAGACCTCG+AGG + chr4_1:43908878-43908897 None:intergenic 55.0%
CCGCCCACCTAAATTGACAC+CGG + chr4_1:43908904-43908923 None:intergenic 55.0%
! CCGGTGTCAATTTAGGTGGG+CGG - chr4_1:43908901-43908920 Msa0549110:intron 55.0%
! CGGTGTCAATTTAGGTGGGC+GGG - chr4_1:43908902-43908921 Msa0549110:intron 55.0%
! GCCGGATACACCAGACGTTT+TGG - chr4_1:43909489-43909508 Msa0549110:CDS 55.0%
TCGAACCTGAGACCTCGAGG+AGG + chr4_1:43908875-43908894 None:intergenic 60.0%
GCGCTTGGGGAAGATCCCAG+TGG + chr4_1:43907386-43907405 None:intergenic 65.0%
! TGGGAGTGTGCTCCTCCTCG+AGG - chr4_1:43908860-43908879 Msa0549110:intron 65.0%
GTGCTCCTCCTCGAGGTCTC+AGG - chr4_1:43908867-43908886 Msa0549110:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 43905060 43909599 43905060 ID=Msa0549110;Name=Msa0549110
chr4_1 mRNA 43905060 43909599 43905060 ID=Msa0549110-mRNA-1;Parent=Msa0549110;Name=Msa0549110-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|0.16|0.07|1|1|1|13|0|477
chr4_1 exon 43909420 43909599 43909420 ID=Msa0549110-mRNA-1:exon:11389;Parent=Msa0549110-mRNA-1
chr4_1 exon 43909104 43909173 43909104 ID=Msa0549110-mRNA-1:exon:11388;Parent=Msa0549110-mRNA-1
chr4_1 exon 43907899 43907957 43907899 ID=Msa0549110-mRNA-1:exon:11387;Parent=Msa0549110-mRNA-1
chr4_1 exon 43907617 43907692 43907617 ID=Msa0549110-mRNA-1:exon:11386;Parent=Msa0549110-mRNA-1
chr4_1 exon 43907423 43907500 43907423 ID=Msa0549110-mRNA-1:exon:11385;Parent=Msa0549110-mRNA-1
chr4_1 exon 43907214 43907301 43907214 ID=Msa0549110-mRNA-1:exon:11384;Parent=Msa0549110-mRNA-1
chr4_1 exon 43906826 43906952 43906826 ID=Msa0549110-mRNA-1:exon:11383;Parent=Msa0549110-mRNA-1
chr4_1 exon 43906625 43906740 43906625 ID=Msa0549110-mRNA-1:exon:11382;Parent=Msa0549110-mRNA-1
chr4_1 exon 43906268 43906491 43906268 ID=Msa0549110-mRNA-1:exon:11381;Parent=Msa0549110-mRNA-1
chr4_1 exon 43906110 43906141 43906110 ID=Msa0549110-mRNA-1:exon:11380;Parent=Msa0549110-mRNA-1
chr4_1 exon 43905894 43905996 43905894 ID=Msa0549110-mRNA-1:exon:11379;Parent=Msa0549110-mRNA-1
chr4_1 exon 43905353 43905461 43905353 ID=Msa0549110-mRNA-1:exon:11378;Parent=Msa0549110-mRNA-1
chr4_1 exon 43905060 43905231 43905060 ID=Msa0549110-mRNA-1:exon:11377;Parent=Msa0549110-mRNA-1
chr4_1 CDS 43909420 43909599 43909420 ID=Msa0549110-mRNA-1:cds;Parent=Msa0549110-mRNA-1
chr4_1 CDS 43909104 43909173 43909104 ID=Msa0549110-mRNA-1:cds;Parent=Msa0549110-mRNA-1
chr4_1 CDS 43907899 43907957 43907899 ID=Msa0549110-mRNA-1:cds;Parent=Msa0549110-mRNA-1
chr4_1 CDS 43907617 43907692 43907617 ID=Msa0549110-mRNA-1:cds;Parent=Msa0549110-mRNA-1
chr4_1 CDS 43907423 43907500 43907423 ID=Msa0549110-mRNA-1:cds;Parent=Msa0549110-mRNA-1
chr4_1 CDS 43907214 43907301 43907214 ID=Msa0549110-mRNA-1:cds;Parent=Msa0549110-mRNA-1
chr4_1 CDS 43906826 43906952 43906826 ID=Msa0549110-mRNA-1:cds;Parent=Msa0549110-mRNA-1
chr4_1 CDS 43906625 43906740 43906625 ID=Msa0549110-mRNA-1:cds;Parent=Msa0549110-mRNA-1
chr4_1 CDS 43906268 43906491 43906268 ID=Msa0549110-mRNA-1:cds;Parent=Msa0549110-mRNA-1
chr4_1 CDS 43906110 43906141 43906110 ID=Msa0549110-mRNA-1:cds;Parent=Msa0549110-mRNA-1
chr4_1 CDS 43905894 43905996 43905894 ID=Msa0549110-mRNA-1:cds;Parent=Msa0549110-mRNA-1
chr4_1 CDS 43905353 43905461 43905353 ID=Msa0549110-mRNA-1:cds;Parent=Msa0549110-mRNA-1
chr4_1 CDS 43905060 43905231 43905060 ID=Msa0549110-mRNA-1:cds;Parent=Msa0549110-mRNA-1
Gene Sequence

>Msa0549110

ATGGCATTCAATGGCCATCAATATTTCCTCTACTTTGGTGTCTTGAATTTTATCATTAGCTCCTTTGTTCCAATAATGGCAGCAGCTGTTACTCCTACAACACTTGTTGACCGTACTTCTCTAAACCGTAGTAGTTTTCCTAATGGTTTCATTTTTGGTACTGCCTCATCCTCTTACCAGTATGAAGGTGCTGCAAATGAAGGTGGAAGAGGACAAAGTATTTGGGATAAATATGCTCATGAACATCCAGAAAAAATATTGGATAGAAGCAATGGAGATGTAGCTGTTGATCAGTATCATCGTTATAAGGAAGATGTTGCAATCATGAAGTATATGAATACAGATGCATACAGATTCTCTATCTCTTGGCCAAGAATATTGCCAAAAGGAAAAGTAAGTGCAGGTATAAATCAAGAAGGAATCAAATATTACAACAATCTGATCAATGAGCTACTTGATAATGGTCTGGTACCATTTGTGACCCTTTTCCACTGGGATCTTCCCCAAGCGCTACAAGACGAGTACAGCGGCTTCTTACGCCCTAATATTATAGTGAAGCATTGGATTACCCTCAATGAGCCACACTCTTACAGTTTGGGTACGGAACCTTACATGGCCTCACACAACCAACTCCTTGCTCATGCAGCTGCTGTCAAACTTTATAGAACCAATCATCAGGCTTCTCAAAATGGTTCAATAGGCATCACCTTAAATTGTCACTGGTTTTTGCCGTTCTCAAATGACACGTTAGATCATCAAGCTGCCGAACGTGCTCTTGATTTCATGTTTGGATGGTTTATGCAGCCACTGACAACAGGAGAGTATCCATCAAGCATGGTGTCTTTCGTGGGAAATCGATTACCAAAGTTCACCAGAGAGCAGTCTAAGATGCTTATTGGTTCATTTGATTTTATTGGAATAAATTACTACACCTCCAATTATGCTTCCAATATACCACAATCAAATAATGACACAAGCACACCTGGATATTTCAAGGATACTCATGTCAATTTAACAACTGAGCGTAACGGGATACCAATTGGACCAAGGGCTGCTTCACCTTGGCTATTTGTTTATCCAAGAGGGATTCAAGAACTGCTGTTGTACACAAAGACAAAGTATAACAATCCTTTGATTTACATAACAGAAAATGGTATGGACGAGTTAAATGATCCAACGCTATCACTTGAAGAGGCACTCATGGATACTTACAGAATCAATTACTTTTATCGACATCTTTATTATATTTCATCTGCAATCAAGCATGGTGTGAAAGTACAAGGATATTTTGCATGGTCACTTTTGGACAATTTTGAATGGCTTGCCGGATACACCAGACGTTTTGGAATTAACTTTGTAGATTACAAAGATAATCTTAAAAGACACCAAAAGCTTTCAGCTCATTGGTTTAGAAATTTTCTTAAAAAGCAGTAG
Protein sequence

>Msa0549110

MAFNGHQYFLYFGVLNFIISSFVPIMAAAVTPTTLVDRTSLNRSSFPNGFIFGTASSSYQYEGAANEGGRGQSIWDKYAHEHPEKILDRSNGDVAVDQYHRYKEDVAIMKYMNTDAYRFSISWPRILPKGKVSAGINQEGIKYYNNLINELLDNGLVPFVTLFHWDLPQALQDEYSGFLRPNIIVKHWITLNEPHSYSLGTEPYMASHNQLLAHAAAVKLYRTNHQASQNGSIGITLNCHWFLPFSNDTLDHQAAERALDFMFGWFMQPLTTGEYPSSMVSFVGNRLPKFTREQSKMLIGSFDFIGINYYTSNYASNIPQSNNDTSTPGYFKDTHVNLTTERNGIPIGPRAASPWLFVYPRGIQELLLYTKTKYNNPLIYITENGMDELNDPTLSLEEALMDTYRINYFYRHLYYISSAIKHGVKVQGYFAWSLLDNFEWLAGYTRRFGINFVDYKDNLKRHQKLSAHWFRNFLKKQ*