AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0645410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0645410 MtrunA17_Chr4g0033261 91.903 494 23 1 1 477 1 494 0.0 942
Msa0645410 MtrunA17_Chr4g0033231 86.134 476 49 1 19 477 19 494 0.0 855
Msa0645410 MtrunA17_Chr2g0329201 66.004 503 125 4 17 476 8 507 0.0 675
Msa0645410 MtrunA17_Chr2g0329191 64.155 491 128 5 29 476 24 509 0.0 647
Msa0645410 MtrunA17_Chr6g0482071 63.035 514 139 6 9 477 7 514 0.0 641
Msa0645410 MtrunA17_Chr4g0006111 65.768 482 134 7 23 476 47 525 0.0 634
Msa0645410 MtrunA17_Chr6g0482131 61.829 503 147 4 18 477 13 513 0.0 630
Msa0645410 MtrunA17_Chr4g0033271 60.672 506 152 4 14 476 10 511 0.0 615
Msa0645410 MtrunA17_Chr6g0482081 61.431 503 150 4 18 477 13 514 0.0 611
Msa0645410 MtrunA17_Chr3g0088441 57.885 520 171 4 1 477 1 515 0.0 611
Msa0645410 MtrunA17_Chr4g0033201 58.893 506 160 4 17 476 9 512 0.0 609
Msa0645410 MtrunA17_Chr4g0033341 59.082 501 162 4 17 476 45 543 0.0 608
Msa0645410 MtrunA17_Chr4g0033311 57.308 520 175 4 1 477 1 516 0.0 605
Msa0645410 MtrunA17_Chr4g0033401 59.961 507 152 4 17 474 4 508 0.0 603
Msa0645410 MtrunA17_Chr2g0329321 60.852 493 120 5 27 476 26 488 0.0 600
Msa0645410 MtrunA17_Chr4g0033191 59.109 494 154 4 29 476 28 519 0.0 600
Msa0645410 MtrunA17_Chr4g0033321 59.136 509 158 4 17 477 4 510 0.0 599
Msa0645410 MtrunA17_Chr4g0033331 59.557 497 156 3 21 474 19 513 0.0 598
Msa0645410 MtrunA17_Chr4g0006131 63.505 485 147 9 18 475 19 500 0.0 594
Msa0645410 MtrunA17_Chr4g0010501 55.625 480 164 4 39 476 22 494 0.0 541
Msa0645410 MtrunA17_Chr5g0430451 54.808 520 147 6 17 476 7 498 0.0 540
Msa0645410 MtrunA17_Chr2g0301231 66.244 394 105 7 109 476 1 392 0.0 511
Msa0645410 MtrunA17_Chr3g0087261 49.064 481 196 5 39 476 35 509 9.59e-165 475
Msa0645410 MtrunA17_Chr3g0087271 47.461 512 220 5 7 476 6 510 1.65e-162 469
Msa0645410 MtrunA17_Chr4g0043091 47.327 505 206 8 23 475 6 502 1.72e-157 456
Msa0645410 MtrunA17_Chr4g0042171 46.804 485 211 5 35 475 18 499 2.05e-149 436
Msa0645410 MtrunA17_Chr3g0090131 46.809 470 210 7 40 475 20 483 7.52e-149 434
Msa0645410 MtrunA17_Chr3g0090111 46.695 469 209 8 41 475 19 480 2.18e-148 432
Msa0645410 MtrunA17_Chr6g0482941 41.923 520 251 8 1 477 2 513 1.86e-142 418
Msa0645410 MtrunA17_Chr6g0482951 42.442 516 242 9 6 477 9 513 2.60e-141 415
Msa0645410 MtrunA17_Chr4g0074561 43.340 473 222 5 46 474 40 510 4.96e-134 397
Msa0645410 MtrunA17_Chr2g0288071 42.801 507 235 8 18 477 45 543 6.64e-134 397
Msa0645410 MtrunA17_Chr4g0074551 44.676 479 210 10 46 477 55 525 1.72e-127 382
Msa0645410 MtrunA17_Chr2g0317841 42.348 477 229 7 40 474 31 503 4.02e-126 377
Msa0645410 MtrunA17_Chr2g0317821 42.292 480 223 8 41 474 32 503 3.00e-125 375
Msa0645410 MtrunA17_Chr2g0288021 38.976 508 253 9 17 477 13 510 1.34e-118 357
Msa0645410 MtrunA17_Chr7g0242691 39.960 498 239 9 25 477 14 496 2.60e-118 357
Msa0645410 MtrunA17_Chr4g0006971 40.452 487 221 10 40 477 26 492 7.37e-113 342
Msa0645410 MtrunA17_Chr3g0087201 52.978 319 115 4 185 476 34 344 6.66e-112 334
Msa0645410 MtrunA17_Chr7g0242681 39.834 482 228 10 41 477 38 502 1.05e-111 340
Msa0645410 MtrunA17_Chr7g0234091 40.466 472 230 7 42 476 76 533 2.26e-107 329
Msa0645410 MtrunA17_Chr5g0428831 38.797 482 227 9 42 475 28 489 2.62e-107 328
Msa0645410 MtrunA17_Chr1g0195611 39.167 480 227 9 42 475 28 488 3.56e-106 325
Msa0645410 MtrunA17_Chr7g0234111 40.764 471 226 7 42 474 76 531 6.54e-105 323
Msa0645410 MtrunA17_Chr7g0234131 38.136 472 238 7 42 474 76 532 2.09e-100 311
Msa0645410 MtrunA17_Chr5g0418871 47.826 299 110 5 60 315 2 297 1.62e-89 276
Msa0645410 MtrunA17_Chr1g0195641 40.103 389 175 7 43 386 29 404 2.81e-86 270
Msa0645410 MtrunA17_Chr7g0230651 39.344 366 173 6 96 424 26 379 2.20e-81 257
Msa0645410 MtrunA17_Chr3g0087191 63.636 165 60 0 22 186 3 167 1.25e-73 229
Msa0645410 MtrunA17_Chr7g0227561 36.882 263 104 4 42 268 47 283 9.76e-52 177
Msa0645410 MtrunA17_Chr7g0236731 40.756 238 97 4 130 323 28 265 9.19e-43 153
Msa0645410 MtrunA17_Chr5g0418861 52.222 90 42 1 386 474 1 90 7.06e-26 100
Msa0645410 MtrunA17_Chr4g0068871 26.010 396 213 13 105 470 183 528 2.78e-21 97.1
Msa0645410 MtrunA17_Chr8g0352881 43.299 97 55 0 225 321 58 154 5.33e-20 87.0
Msa0645410 MtrunA17_Chr8g0352881 51.852 54 25 1 50 103 7 59 3.09e-12 64.7
Msa0645410 MtrunA17_Chr2g0301221 70.175 57 16 1 26 81 49 105 1.62e-14 69.7
Msa0645410 MtrunA17_Chr7g0227591 51.923 52 25 0 423 474 14 65 3.87e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0645410 AT2G44450.1 50.625 480 187 5 41 477 31 503 1.48e-170 490
Msa0645410 AT5G44640.1 48.141 511 210 7 11 477 5 504 5.38e-169 486
Msa0645410 AT2G44480.1 48.975 488 205 3 29 474 23 508 2.87e-168 484
Msa0645410 AT2G44480.3 48.975 488 205 3 29 474 54 539 1.00e-167 484
Msa0645410 AT5G42260.1 48.814 506 202 8 10 470 4 497 1.94e-167 482
Msa0645410 AT3G60130.1 50.000 482 190 6 38 475 27 501 2.87e-164 474
Msa0645410 AT5G24550.1 46.863 510 221 8 14 476 5 511 5.93e-155 451
Msa0645410 AT2G25630.1 46.850 508 197 8 11 474 5 483 9.43e-155 449
Msa0645410 AT1G26560.1 48.536 478 199 5 41 474 32 506 1.24e-153 447
Msa0645410 AT5G54570.1 46.584 483 212 4 36 474 25 505 2.29e-152 444
Msa0645410 AT5G24540.1 46.863 510 221 8 14 476 5 511 7.99e-149 436
Msa0645410 AT5G36890.1 47.010 485 212 7 25 475 7 480 1.29e-148 433
Msa0645410 AT5G36890.2 47.010 485 212 7 25 475 7 480 1.52e-148 433
Msa0645410 AT3G60120.1 47.541 488 200 10 38 476 11 491 5.62e-147 431
Msa0645410 AT3G18070.3 46.170 470 212 8 41 476 30 492 1.19e-146 429
Msa0645410 AT3G60140.1 43.555 512 230 8 11 476 6 504 4.98e-146 430
Msa0645410 AT2G44490.1 45.510 490 216 8 34 475 7 493 2.58e-145 427
Msa0645410 AT1G51470.1 46.122 477 208 6 40 476 44 511 4.67e-145 425
Msa0645410 AT1G47600.1 45.493 477 211 6 40 476 44 511 1.92e-144 424
Msa0645410 AT1G47600.2 45.493 477 210 6 40 476 44 510 2.96e-144 423
Msa0645410 AT3G18070.1 45.493 477 213 8 40 476 29 498 1.03e-143 421
Msa0645410 AT3G18080.1 45.303 479 213 8 40 476 38 509 6.73e-141 414
Msa0645410 AT2G44470.3 42.829 509 237 9 14 476 7 507 2.90e-140 416
Msa0645410 AT2G44460.1 43.836 511 232 10 10 474 3 504 6.17e-136 404
Msa0645410 AT2G44480.2 46.814 408 173 3 109 474 1 406 1.13e-134 395
Msa0645410 AT3G60130.2 48.921 417 162 6 103 475 40 449 1.15e-134 397
Msa0645410 AT1G66280.1 42.967 519 233 13 7 474 4 510 4.45e-130 387
Msa0645410 AT1G75940.1 44.559 487 211 10 41 474 37 517 5.10e-130 387
Msa0645410 AT3G03640.1 39.922 516 255 8 9 476 6 514 1.03e-129 387
Msa0645410 AT5G54570.3 44.988 429 191 3 36 421 25 451 1.73e-129 383
Msa0645410 AT3G09260.1 42.531 482 227 8 41 476 35 512 7.27e-129 384
Msa0645410 AT1G61820.1 41.016 512 249 9 9 476 4 506 1.21e-128 383
Msa0645410 AT3G21370.1 43.058 497 222 11 30 475 26 512 2.33e-128 383
Msa0645410 AT5G25980.2 41.243 531 248 14 1 475 1 523 9.59e-128 382
Msa0645410 AT1G52400.3 42.685 499 225 11 30 477 30 518 5.85e-127 379
Msa0645410 AT1G52400.1 42.685 499 225 11 30 477 30 518 5.85e-127 379
Msa0645410 AT1G66270.1 42.600 500 228 10 30 476 19 512 6.93e-127 379
Msa0645410 AT2G32860.2 40.909 484 238 6 40 477 93 574 6.44e-126 380
Msa0645410 AT2G32860.1 41.116 484 236 7 40 477 93 573 1.04e-125 379
Msa0645410 AT3G60130.3 47.482 417 157 7 103 475 40 438 1.65e-125 373
Msa0645410 AT5G26000.1 41.176 527 227 14 15 474 1 511 2.60e-125 375
Msa0645410 AT5G28510.1 41.365 498 231 10 30 474 30 519 3.50e-124 372
Msa0645410 AT1G66270.2 42.400 500 227 11 30 476 19 510 7.96e-124 371
Msa0645410 AT5G24540.2 45.475 453 198 7 14 420 5 454 4.15e-123 367
Msa0645410 AT1G61810.1 41.667 480 233 8 41 476 33 509 1.70e-119 360
Msa0645410 AT1G02850.2 40.984 488 218 10 36 475 21 486 2.60e-119 358
Msa0645410 AT2G44470.1 41.814 452 210 8 14 420 7 450 1.09e-117 353
Msa0645410 AT5G54570.2 44.878 410 180 4 109 474 1 408 1.40e-117 352
Msa0645410 AT1G02850.5 39.335 511 217 11 36 475 21 509 5.13e-115 348
Msa0645410 AT4G21760.2 39.208 505 254 8 15 474 25 521 2.08e-114 348
Msa0645410 AT1G61810.3 41.756 467 225 8 41 463 33 496 2.09e-114 348
Msa0645410 AT4G27830.1 39.442 502 236 10 18 474 6 484 2.64e-114 346
Msa0645410 AT3G60140.2 42.619 420 183 7 11 385 6 412 6.63e-114 343
Msa0645410 AT1G02850.4 39.258 512 217 12 36 475 21 510 3.91e-113 343
Msa0645410 AT2G44450.2 46.797 359 141 5 162 477 1 352 4.33e-112 335
Msa0645410 AT5G25980.3 40.842 475 219 12 1 421 1 467 6.67e-111 336
Msa0645410 AT5G25980.1 40.842 475 219 12 1 421 1 467 6.67e-111 336
Msa0645410 AT5G26000.2 40.678 472 199 12 15 421 1 456 1.62e-108 330
Msa0645410 AT4G27820.1 39.917 481 223 9 40 474 22 482 1.86e-108 331
Msa0645410 AT4G21760.1 38.416 505 248 9 15 474 25 511 1.89e-108 332
Msa0645410 AT3G62750.1 38.163 490 244 10 22 474 6 473 2.18e-107 328
Msa0645410 AT3G62750.2 38.163 490 244 10 22 474 32 499 3.73e-107 328
Msa0645410 AT1G02850.1 39.139 488 200 11 36 475 21 459 5.88e-107 326
Msa0645410 AT3G18070.2 40.046 437 218 8 40 476 29 421 1.85e-106 323
Msa0645410 AT4G22100.1 37.860 486 240 10 34 475 16 483 3.38e-105 323
Msa0645410 AT2G44480.4 50.161 311 113 2 29 297 54 364 2.41e-103 314
Msa0645410 AT3G62740.1 36.253 491 251 10 25 474 9 478 2.01e-102 315
Msa0645410 AT1G60090.2 35.833 480 247 8 41 477 24 485 7.54e-102 314
Msa0645410 AT1G52400.2 41.043 441 200 10 30 420 30 460 1.50e-101 312
Msa0645410 AT1G60090.1 34.774 486 251 9 41 477 24 492 4.26e-99 307
Msa0645410 AT1G45191.4 36.590 481 236 9 42 477 30 486 4.60e-99 307
Msa0645410 AT1G45191.6 36.590 481 247 8 42 477 30 497 1.84e-98 306
Msa0645410 AT1G45191.2 35.908 479 248 7 42 477 30 492 1.43e-97 303
Msa0645410 AT2G44470.2 40.967 393 179 8 14 361 7 391 2.01e-97 299
Msa0645410 AT5G48375.1 39.326 445 185 12 25 439 22 411 4.79e-97 300
Msa0645410 AT1G45191.1 36.270 488 251 9 42 477 30 509 7.00e-97 302
Msa0645410 AT2G44480.5 48.727 275 139 1 200 474 30 302 9.98e-95 289
Msa0645410 AT1G61810.5 40.049 412 200 8 109 476 1 409 9.46e-92 285
Msa0645410 AT3G18070.4 43.529 340 148 6 41 340 30 365 6.42e-91 282
Msa0645410 AT3G62750.4 37.130 439 218 9 21 423 31 447 1.38e-90 283
Msa0645410 AT1G61810.4 40.874 389 184 7 41 385 33 419 2.51e-90 282
Msa0645410 AT1G02850.3 39.048 420 170 9 36 385 21 424 4.23e-89 279
Msa0645410 AT3G18070.7 48.014 277 140 3 200 476 37 309 5.14e-89 275
Msa0645410 AT4G27830.2 39.173 411 190 8 109 474 1 396 2.34e-88 276
Msa0645410 AT3G62740.2 36.009 436 218 9 25 420 9 423 6.10e-87 273
Msa0645410 AT4G27820.2 38.592 412 192 8 109 474 1 397 1.30e-82 262
Msa0645410 AT4G27820.3 38.592 412 192 8 109 474 1 397 1.30e-82 262
Msa0645410 AT2G44460.2 42.659 361 158 7 160 474 1 358 2.82e-81 259
Msa0645410 AT3G62750.7 39.759 332 157 5 22 319 6 328 3.89e-81 256
Msa0645410 AT3G62750.8 39.759 332 157 5 22 319 6 328 3.89e-81 256
Msa0645410 AT1G60270.1 38.710 341 161 4 41 339 24 358 2.30e-80 254
Msa0645410 AT3G62750.3 39.759 332 157 5 22 319 32 354 2.61e-80 256
Msa0645410 AT1G51490.1 41.860 344 148 8 50 352 29 361 4.83e-80 255
Msa0645410 AT3G62750.6 36.973 403 202 8 109 474 1 388 1.53e-79 253
Msa0645410 AT3G62750.5 36.973 403 202 8 109 474 1 388 1.53e-79 253
Msa0645410 AT1G61820.3 38.525 366 179 7 155 476 4 367 4.73e-78 248
Msa0645410 AT1G45191.5 36.311 347 172 4 42 346 30 369 9.58e-73 236
Msa0645410 AT1G45191.3 35.912 362 181 5 42 359 30 384 2.34e-72 235
Msa0645410 AT3G18070.5 36.599 347 179 6 40 386 29 334 4.63e-71 229
Msa0645410 AT1G61810.2 42.751 269 111 4 41 267 33 300 2.85e-67 218
Msa0645410 AT3G18070.6 45.455 187 101 1 200 386 37 222 5.13e-54 181
Msa0645410 AT1G60270.2 36.630 273 129 3 109 339 1 271 6.70e-53 180
Msa0645410 AT5G16580.1 33.829 269 145 5 185 431 42 299 4.37e-41 149
Msa0645410 AT3G06510.1 25.369 406 215 17 104 475 159 510 5.52e-20 93.6
Msa0645410 AT3G06510.2 25.708 424 225 19 104 475 159 544 1.08e-19 92.8

Find 93 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGCAACTTACATAATATT+AGG 0.191120 4_3:-50604145 None:intergenic
GTGGAAGAGGACAAAGTATT+TGG 0.192835 4_3:+50602216 Msa0645410:CDS
TTGATTTACATAACAGAAAA+TGG 0.216843 4_3:+50605454 Msa0645410:CDS
GAGCAGTCTAAGATGCTTAT+TGG 0.240397 4_3:+50604960 Msa0645410:CDS
TGGCATCAGGCTTCTCAAAA+TGG 0.265050 4_3:+50604620 Msa0645410:intron
TTATTACTTGGCAATATTCT+TGG 0.269887 4_3:-50603737 None:intergenic
GTAACAGCTGCTGCCATTAT+TGG 0.270052 4_3:-50601837 None:intergenic
TGGTATATTGGAAGCATAAT+TGG 0.270855 4_3:-50605018 None:intergenic
CGTGCTCTTGATTTCATGTT+TGG 0.298768 4_3:+50604719 Msa0645410:CDS
ACCAATGAGCTGAAAGCTTT+TGG 0.307343 4_3:-50606260 None:intergenic
ATTATTTGATTGTGGTATAT+TGG 0.312657 4_3:-50605030 None:intergenic
TAGCTCCTTTGTTCCAATAA+TGG 0.326020 4_3:+50601824 Msa0645410:CDS
GCCGGATACACCAGACGTTT+TGG 0.335510 4_3:+50606198 Msa0645410:CDS
ATCAATGAGCTACTTGATAA+TGG 0.365239 4_3:+50603925 Msa0645410:CDS
CATCAATATTTCCTCTACTT+TGG 0.365921 4_3:+50601783 Msa0645410:CDS
TGAGCCACACTCTTACAGTT+TGG 0.367434 4_3:+50604441 Msa0645410:CDS
ACCAAAAGCTTTCAGCTCAT+TGG 0.373860 4_3:+50606259 Msa0645410:CDS
GCTTCTCAAAATGGTTCAAT+AGG 0.392323 4_3:+50604629 Msa0645410:CDS
TCCAAAACGTCTGGTGTATC+CGG 0.394369 4_3:-50606199 None:intergenic
ATGCAACTTACATAATATTA+GGG 0.399717 4_3:-50604144 None:intergenic
ACGAAAGACACCATGCTTGA+TGG 0.404375 4_3:-50604909 None:intergenic
CATGAAATCAAGAGCACGTT+CGG 0.407930 4_3:-50604714 None:intergenic
GTACTCGTCTTGTAGCGCTT+GGG 0.413064 4_3:-50604107 None:intergenic
GAGCCACACTCTTACAGTTT+GGG 0.414537 4_3:+50604442 Msa0645410:CDS
TGGAAGAGGACAAAGTATTT+GGG 0.414779 4_3:+50602217 Msa0645410:CDS
TCGGTGTTGCACCTGATGAT+TGG 0.418117 4_3:-50604533 None:intergenic
GGGGAAGATCCCAGTGGAAA+AGG 0.421057 4_3:-50604087 None:intergenic
TTACTCACCCTTGGTCCAAT+TGG 0.424891 4_3:-50605245 None:intergenic
TACAAAACGCACCTGGTAAG+AGG 0.428857 4_3:-50601937 None:intergenic
TGATCAGTATCATCGTTATA+AGG 0.429927 4_3:+50603449 Msa0645410:CDS
TGTACTCGTCTTGTAGCGCT+TGG 0.432452 4_3:-50604108 None:intergenic
GACTGCTCTCTGGTGAACTT+TGG 0.446094 4_3:-50604945 None:intergenic
GAGCGTAACGGGATACCAAT+TGG 0.457796 4_3:+50605230 Msa0645410:CDS
TTTATGCAGCCACTGACAAC+AGG 0.464763 4_3:+50604879 Msa0645410:CDS
AGCAGTTCTTGAATCCCTCT+TGG 0.465436 4_3:-50605400 None:intergenic
CAAAGTAGAGGAAATATTGA+TGG 0.467715 4_3:-50601782 None:intergenic
CATACAGATTCTCTATCTCT+TGG 0.469897 4_3:+50603714 Msa0645410:CDS
AAAGTTAATTCCAAAACGTC+TGG 0.470833 4_3:-50606208 None:intergenic
TTTGGGTACGGAACCTTACA+TGG 0.476476 4_3:+50604459 Msa0645410:CDS
ATCACTTGAAGAGGCACTCA+TGG 0.486552 4_3:+50605935 Msa0645410:CDS
ATCAAGCATGGTGTCTTTCG+TGG 0.490440 4_3:+50604911 Msa0645410:CDS
GTACGGTCAACAAGTGTTGT+AGG 0.490892 4_3:-50601861 None:intergenic
TGGTTGTGTGAGGCCATGTA+AGG 0.493958 4_3:-50604472 None:intergenic
CACAAGGGCATGGCATTCAA+TGG 0.496087 4_3:+50601759 None:intergenic
GGCTATTTGTTTATCCAAGA+GGG 0.499794 4_3:+50605386 Msa0645410:CDS
TCGGAGACAGAGTGAAGCAT+TGG 0.500114 4_3:+50604407 Msa0645410:intron
GCATCACCTTAAATTGTCAC+TGG 0.506655 4_3:+50604651 Msa0645410:CDS
TACTGCCTCATCCTCTTACC+AGG 0.507287 4_3:+50601926 Msa0645410:CDS
TGAGTATCCTTGAAATATCC+AGG 0.513235 4_3:-50605065 None:intergenic
GGGAAGATCCCAGTGGAAAA+GGG 0.516334 4_3:-50604086 None:intergenic
GTAAGAGTGTGGCTCATTGA+GGG 0.516603 4_3:-50604434 None:intergenic
CTCTTGATTTCATGTTTGGA+TGG 0.519547 4_3:+50604723 Msa0645410:CDS
AAAATATTGGATAGAAGCAA+TGG 0.520670 4_3:+50603414 Msa0645410:CDS
ACGCACCTGGTAAGAGGATG+AGG 0.521862 4_3:-50601931 None:intergenic
AATAATGACACAAGCACACC+TGG 0.522457 4_3:+50605047 Msa0645410:CDS
GCTGCAAATGAAGGTGGAAG+AGG 0.523453 4_3:+50602203 Msa0645410:CDS
AAGCATCTTAGACTGCTCTC+TGG 0.526279 4_3:-50604955 None:intergenic
AAGCACACCTGGATATTTCA+AGG 0.530555 4_3:+50605058 Msa0645410:CDS
CAGCTGCATGAGCAAGGAGT+TGG 0.531565 4_3:-50604492 None:intergenic
ACTTTATAGAACCAATCATC+AGG 0.533812 4_3:+50604522 Msa0645410:CDS
TGTAAGAGTGTGGCTCATTG+AGG 0.538006 4_3:-50604435 None:intergenic
TACTCGTCTTGTAGCGCTTG+GGG 0.539586 4_3:-50604106 None:intergenic
CAGTGGAAAAGGGTCACAAA+TGG 0.545800 4_3:-50604076 None:intergenic
CTTGTGTCATTATTTGATTG+TGG 0.555807 4_3:-50605038 None:intergenic
TGACAGCAGCTGCATGAGCA+AGG 0.559687 4_3:-50604498 None:intergenic
AAATATGCTCATGAACATCC+AGG 0.571960 4_3:+50602242 Msa0645410:CDS
GTGTTGATAATGTAGTATGA+AGG 0.577599 4_3:+50602179 Msa0645410:intron
TGCTGCCATTATTGGAACAA+AGG 0.578034 4_3:-50601829 None:intergenic
TATGAAGGTGCTGCAAATGA+AGG 0.579768 4_3:+50602194 Msa0645410:CDS
GCAGAAGGAAAAGTAAGTGC+AGG 0.581251 4_3:+50603865 Msa0645410:intron
AGGCATGGTGTGAAAGTACA+AGG 0.584642 4_3:+50606135 Msa0645410:intron
TATATTGGAAGCATAATTGG+AGG 0.589479 4_3:-50605015 None:intergenic
AACCATTAGGAAAACTACTA+CGG 0.590332 4_3:-50601893 None:intergenic
TCCAACGCTATCACTTGAAG+AGG 0.591945 4_3:+50605926 Msa0645410:CDS
TGGCTATTTGTTTATCCAAG+AGG 0.593511 4_3:+50605385 Msa0645410:CDS
GCCTCTTCAAGTGATAGCGT+TGG 0.596017 4_3:-50605927 None:intergenic
ACACTCTTACAGTTTGGGTA+CGG 0.597858 4_3:+50604447 Msa0645410:CDS
TCAAGCATGGTGTCTTTCGT+GGG 0.598348 4_3:+50604912 Msa0645410:CDS
GGATACTCTCCTGTTGTCAG+TGG 0.600363 4_3:-50604888 None:intergenic
CGGGATACCAATTGGACCAA+GGG 0.607534 4_3:+50605238 Msa0645410:CDS
TTATGTAGGCTGCTTCACCT+TGG 0.615550 4_3:+50605365 Msa0645410:intron
AGTGCAGGTATAAATCAAGA+AGG 0.620837 4_3:+50603880 Msa0645410:CDS
AATTCAAGACACCAAAGTAG+AGG 0.621817 4_3:-50601794 None:intergenic
CTTGGATAAACAAATAGCCA+AGG 0.631378 4_3:-50605382 None:intergenic
TACTACGGTTTAGAGAAGTA+CGG 0.651396 4_3:-50601878 None:intergenic
AGGAGAGTATCCATCAAGCA+TGG 0.653040 4_3:+50604899 Msa0645410:CDS
GAAGGTGCTGCAAATGAAGG+TGG 0.659790 4_3:+50602197 Msa0645410:CDS
AGCAAGGAGTTGGTTGTGTG+AGG 0.661595 4_3:-50604482 None:intergenic
ACGGGATACCAATTGGACCA+AGG 0.664320 4_3:+50605237 Msa0645410:CDS
GTACCCAAACTGTAAGAGTG+TGG 0.677255 4_3:-50604445 None:intergenic
TCTAACGTGTCATTTGAGAA+CGG 0.686339 4_3:-50604680 None:intergenic
GCGCTACAAGACGAGTACAG+CGG 0.733888 4_3:+50604112 Msa0645410:CDS
GCGCTTGGGGAAGATCCCAG+TGG 0.736847 4_3:-50604093 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTGTTTGTTAAAAAA+AGG + chr4_3:50602659-50602678 Msa0645410:intron 10.0%
!!! TATTTTGTTTGTTAAAAAAA+GGG + chr4_3:50602660-50602679 Msa0645410:intron 10.0%
!!! AAAAAGCAAAAATAAATTTT+AGG + chr4_3:50602712-50602731 Msa0645410:intron 10.0%
!!! AAAATATTTTACATCAAATT+AGG - chr4_3:50605189-50605208 None:intergenic 10.0%
!!! TTTTTTTTTTTTTTAAGAGA+TGG + chr4_3:50605721-50605740 Msa0645410:intron 10.0%
!! CTTAAAAAAAAAAAAAGATT+AGG - chr4_3:50605779-50605798 None:intergenic 10.0%
!!! TTTTTTTTTTTTTAAGAGAT+AGG + chr4_3:50605722-50605741 Msa0645410:intron 10.0%
!! AATATATCAGATATTAAAAT+AGG - chr4_3:50606092-50606111 None:intergenic 10.0%
!! AAATTGAGATAATTAAAGAA+AGG - chr4_3:50602292-50602311 None:intergenic 15.0%
!! TTTCTTTAATTATCTCAATT+TGG + chr4_3:50602291-50602310 Msa0645410:intron 15.0%
!!! CAAGTTATTTTATTTTTGAA+GGG - chr4_3:50602341-50602360 None:intergenic 15.0%
!!! TCAAGTTATTTTATTTTTGA+AGG - chr4_3:50602342-50602361 None:intergenic 15.0%
!!! ATAAGTGATATTTTATGTAT+TGG + chr4_3:50602431-50602450 Msa0645410:intron 15.0%
!! TCTTAACGAAAATTAATTAT+GGG - chr4_3:50602783-50602802 None:intergenic 15.0%
!! ATCTTAACGAAAATTAATTA+TGG - chr4_3:50602784-50602803 None:intergenic 15.0%
!! ACATGATTATATATCTTAAA+TGG + chr4_3:50603215-50603234 Msa0645410:intron 15.0%
!!! TATATTCCTTTAACTTTTTT+TGG - chr4_3:50603284-50603303 None:intergenic 15.0%
!!! AACCATTTTTACATTTTAAT+AGG + chr4_3:50603655-50603674 Msa0645410:intron 15.0%
!! TTCCTATTAAAATGTAAAAA+TGG - chr4_3:50603660-50603679 None:intergenic 15.0%
!!! TAAATAGGATTTTAAATTAC+AGG - chr4_3:50604805-50604824 None:intergenic 15.0%
!!! CAAATTGATTTTTTTATCAA+TGG - chr4_3:50605155-50605174 None:intergenic 15.0%
!!! ATATATCTAAAGTTAGTTAT+TGG + chr4_3:50605538-50605557 Msa0645410:intron 15.0%
!! TTAATAACTAAATTTAGGTA+TGG + chr4_3:50605890-50605909 Msa0645410:intron 15.0%
!!! ATTATTATTTCTGACTTTTT+TGG + chr4_3:50606109-50606128 Msa0645410:intron 15.0%
!!! AAACATACATTAGATGATTT+TGG - chr4_3:50602004-50602023 None:intergenic 20.0%
!! AATTGAAGCTTAAGAAATAT+TGG + chr4_3:50602130-50602149 Msa0645410:intron 20.0%
!! AGAAAAAAAAGAAATATACC+TGG - chr4_3:50602263-50602282 None:intergenic 20.0%
!! CAAACAAAATATTATTGATC+AGG - chr4_3:50602651-50602670 None:intergenic 20.0%
!!! ACCTTAAATTTTCATACATT+AGG + chr4_3:50603094-50603113 Msa0645410:intron 20.0%
!!! CATAACCTACATTTTAATTA+AGG + chr4_3:50603140-50603159 Msa0645410:intron 20.0%
!! TCAAACCTTAATTAAAATGT+AGG - chr4_3:50603148-50603167 None:intergenic 20.0%
!!! ACATATATAGTTCTTTGTTT+GGG - chr4_3:50603257-50603276 None:intergenic 20.0%
!! AAACAAAGAACTATATATGT+GGG + chr4_3:50603257-50603276 Msa0645410:intron 20.0%
!! TTAATGTAGTAATTGCTTAT+TGG + chr4_3:50603374-50603393 Msa0645410:intron 20.0%
!! ATCTCATACAGAAAAAATAT+TGG + chr4_3:50603401-50603420 Msa0645410:intron 20.0%
!!! TGCTTTTATATCAATCAAAT+TGG - chr4_3:50603480-50603499 None:intergenic 20.0%
!!! AATTTTTTGTCTAGTTTATG+TGG + chr4_3:50603631-50603650 Msa0645410:intron 20.0%
!!! ATTCAACATTTGTTATTACT+TGG - chr4_3:50603752-50603771 None:intergenic 20.0%
!! TATAGATAAAATGAAACTCT+AGG - chr4_3:50603798-50603817 None:intergenic 20.0%
!!! TAGAGTTTCATTTTATCTAT+AGG + chr4_3:50603797-50603816 Msa0645410:intron 20.0%
!! ATCATGGTAATATAAATTGA+TGG + chr4_3:50604031-50604050 Msa0645410:intron 20.0%
!! ATGCAACTTACATAATATTA+GGG - chr4_3:50604147-50604166 None:intergenic 20.0%
!! AAGCAATAATAATAAATCGA+TGG + chr4_3:50604184-50604203 Msa0645410:intron 20.0%
!! GAAATTAACAAGTAAAACAT+CGG - chr4_3:50604333-50604352 None:intergenic 20.0%
!! AATCATAACAAATACAAGAA+AGG - chr4_3:50604570-50604589 None:intergenic 20.0%
!!! ATTGGTTCATTTGATTTTAT+TGG + chr4_3:50604978-50604997 Msa0645410:CDS 20.0%
!! ATTATTTGATTGTGGTATAT+TGG - chr4_3:50605033-50605052 None:intergenic 20.0%
!!! TTTTCTGTTATGTAAATCAA+AGG - chr4_3:50605454-50605473 None:intergenic 20.0%
!! TTGATTTACATAACAGAAAA+TGG + chr4_3:50605454-50605473 Msa0645410:CDS 20.0%
!! TGCATATTGTAAAAATCAAA+TGG - chr4_3:50605497-50605516 None:intergenic 20.0%
!!! ATTATACAATTGATTTGACT+AGG - chr4_3:50605859-50605878 None:intergenic 20.0%
!! TTTCCTAATGGTTTCATTTT+TGG + chr4_3:50601903-50601922 Msa0645410:CDS 25.0%
!!! TACTTTAAGACCAAAGATTT+AGG + chr4_3:50602090-50602109 Msa0645410:intron 25.0%
!!! ACTTTAAGACCAAAGATTTA+GGG + chr4_3:50602091-50602110 Msa0645410:intron 25.0%
!!! ATATTGGAGGTTAATTTGTA+AGG + chr4_3:50602146-50602165 Msa0645410:intron 25.0%
! CAAAATAGAGTACATGAATA+CGG - chr4_3:50602535-50602554 None:intergenic 25.0%
! TTTAACCCTATTGTTATATG+TGG + chr4_3:50602855-50602874 Msa0645410:intron 25.0%
! AAACAAACTAATGAAGTCAT+CGG - chr4_3:50602885-50602904 None:intergenic 25.0%
! GCCTAATGTATGAAAATTTA+AGG - chr4_3:50603098-50603117 None:intergenic 25.0%
! CACATATATAGTTCTTTGTT+TGG - chr4_3:50603258-50603277 None:intergenic 25.0%
! CAAACAAAGAACTATATATG+TGG + chr4_3:50603256-50603275 Msa0645410:intron 25.0%
!! TGAAAGTAGTCTTTTTACTT+TGG + chr4_3:50603342-50603361 Msa0645410:intron 25.0%
! AAAATATTGGATAGAAGCAA+TGG + chr4_3:50603414-50603433 Msa0645410:CDS 25.0%
! CAAAATATTAGAGCTTCATA+AGG - chr4_3:50603606-50603625 None:intergenic 25.0%
! TTATTACTTGGCAATATTCT+TGG - chr4_3:50603740-50603759 None:intergenic 25.0%
! AAACTCTAGGTGAATTAAAA+TGG - chr4_3:50603785-50603804 None:intergenic 25.0%
! CTAAATGATGTTCATAATCA+TGG + chr4_3:50604015-50604034 Msa0645410:intron 25.0%
! ATGGTAATATAAATTGATGG+AGG + chr4_3:50604034-50604053 Msa0645410:intron 25.0%
! CATGCAACTTACATAATATT+AGG - chr4_3:50604148-50604167 None:intergenic 25.0%
! CTAATATTATGTAAGTTGCA+TGG + chr4_3:50604146-50604165 Msa0645410:intron 25.0%
! ACAAATACAAGAAAGGTTTA+TGG - chr4_3:50604563-50604582 None:intergenic 25.0%
!! TCGAGTTAGTTAAAGTTTTT+CGG - chr4_3:50604595-50604614 None:intergenic 25.0%
! CTTTAACTAACTCGAATTAA+TGG + chr4_3:50604600-50604619 Msa0645410:intron 25.0%
! CCATAATCATGCATATAAAT+AGG - chr4_3:50604820-50604839 None:intergenic 25.0%
! CCTATTTATATGCATGATTA+TGG + chr4_3:50604817-50604836 Msa0645410:intron 25.0%
!!! TTCTTGAAAGACTTTTTTGT+AGG + chr4_3:50604856-50604875 Msa0645410:intron 25.0%
!!! ATGTTATTTCAATTCCTCAT+AGG - chr4_3:50605128-50605147 None:intergenic 25.0%
! TCAGTATACGATTGTATAAA+TGG - chr4_3:50605335-50605354 None:intergenic 25.0%
!! AATTTTCATTCTTAAGAGAG+AGG + chr4_3:50605687-50605706 Msa0645410:intron 25.0%
! CTCTCTTAAGAAAAAAGATT+AGG - chr4_3:50605750-50605769 None:intergenic 25.0%
!!! AATCTTTTTTCTTAAGAGAG+AGG + chr4_3:50605750-50605769 Msa0645410:intron 25.0%
! TATTGAGGGTAAAAAAGATT+AGG - chr4_3:50605812-50605831 None:intergenic 25.0%
! AGAATCAAACAATGTATTGA+GGG - chr4_3:50605826-50605845 None:intergenic 25.0%
! AAGAATCAAACAATGTATTG+AGG - chr4_3:50605827-50605846 None:intergenic 25.0%
! ATATGAAATAAGTAGAGAGA+TGG - chr4_3:50606029-50606048 None:intergenic 25.0%
! TAAAATAGGCAAACTAAGTA+AGG - chr4_3:50606078-50606097 None:intergenic 25.0%
!!! ATTTCTGACTTTTTTGGTTA+AGG + chr4_3:50606115-50606134 Msa0645410:intron 25.0%
CAAAGTAGAGGAAATATTGA+TGG - chr4_3:50601785-50601804 None:intergenic 30.0%
CATCAATATTTCCTCTACTT+TGG + chr4_3:50601783-50601802 Msa0645410:CDS 30.0%
AACCATTAGGAAAACTACTA+CGG - chr4_3:50601896-50601915 None:intergenic 30.0%
GTACCAAAAATGAAACCATT+AGG - chr4_3:50601909-50601928 None:intergenic 30.0%
! AAAATTTTACAAAACGCACC+TGG - chr4_3:50601947-50601966 None:intergenic 30.0%
TGAAGCTTAAGAAATATTGG+AGG + chr4_3:50602133-50602152 Msa0645410:intron 30.0%
!! GTGTTGATAATGTAGTATGA+AGG + chr4_3:50602179-50602198 Msa0645410:intron 30.0%
! CAATTTGGTGAATAATAGCT+TGG + chr4_3:50602306-50602325 Msa0645410:intron 30.0%
GATAGTATGTAGCTTATAAG+TGG + chr4_3:50602392-50602411 Msa0645410:intron 30.0%
ATGTAGCTTATAAGTGGAAA+AGG + chr4_3:50602398-50602417 Msa0645410:intron 30.0%
TGTAGCTTATAAGTGGAAAA+GGG + chr4_3:50602399-50602418 Msa0645410:intron 30.0%
!! TTTATGTATTGGTCTTGTTC+TGG + chr4_3:50602442-50602461 Msa0645410:intron 30.0%
TGTAGTCATAATACTTATGC+TGG + chr4_3:50602580-50602599 Msa0645410:intron 30.0%
AATTGAACCAACTTGACAAA+CGG - chr4_3:50602826-50602845 None:intergenic 30.0%
AATATCGCATGAGAAAGAAT+AGG + chr4_3:50603058-50603077 Msa0645410:intron 30.0%
CATGAGAAAGAATAGGAATT+GGG + chr4_3:50603065-50603084 Msa0645410:intron 30.0%
ATGAGAAAGAATAGGAATTG+GGG + chr4_3:50603066-50603085 Msa0645410:intron 30.0%
TAAGGTTTGAATTACAACTG+AGG + chr4_3:50603158-50603177 Msa0645410:intron 30.0%
AAGGTTTGAATTACAACTGA+GGG + chr4_3:50603159-50603178 Msa0645410:intron 30.0%
TGATCAGTATCATCGTTATA+AGG + chr4_3:50603449-50603468 Msa0645410:CDS 30.0%
ATCAATGAGCTACTTGATAA+TGG + chr4_3:50603925-50603944 Msa0645410:CDS 30.0%
GATCAAATACTTGTGAAACA+TGG - chr4_3:50603965-50603984 None:intergenic 30.0%
! CATATGCAGAAAAGATTTTC+AGG + chr4_3:50604357-50604376 Msa0645410:intron 30.0%
ACTTTATAGAACCAATCATC+AGG + chr4_3:50604522-50604541 Msa0645410:CDS 30.0%
AAGAAAGGTTTATGGTTCTT+CGG - chr4_3:50604555-50604574 None:intergenic 30.0%
CAAAAACCAGTGACAATTTA+AGG - chr4_3:50604660-50604679 None:intergenic 30.0%
! TATATTGGAAGCATAATTGG+AGG - chr4_3:50605018-50605037 None:intergenic 30.0%
! TGGTATATTGGAAGCATAAT+TGG - chr4_3:50605021-50605040 None:intergenic 30.0%
! CTTGTGTCATTATTTGATTG+TGG - chr4_3:50605041-50605060 None:intergenic 30.0%
! TCTAAAGTTAGTTATTGGCT+TGG + chr4_3:50605543-50605562 Msa0645410:intron 30.0%
!! CTAAAGTTAGTTATTGGCTT+GGG + chr4_3:50605544-50605563 Msa0645410:intron 30.0%
! AAGTACAAGGATATTTTGCA+TGG + chr4_3:50606148-50606167 Msa0645410:CDS 30.0%
!! ATATTTTGCATGGTCACTTT+TGG + chr4_3:50606158-50606177 Msa0645410:CDS 30.0%
! CACTTTTGGACAATTTTGAA+TGG + chr4_3:50606172-50606191 Msa0645410:CDS 30.0%
AAAGTTAATTCCAAAACGTC+TGG - chr4_3:50606211-50606230 None:intergenic 30.0%
AATTCAAGACACCAAAGTAG+AGG - chr4_3:50601797-50601816 None:intergenic 35.0%
TAGCTCCTTTGTTCCAATAA+TGG + chr4_3:50601824-50601843 Msa0645410:CDS 35.0%
TACTACGGTTTAGAGAAGTA+CGG - chr4_3:50601881-50601900 None:intergenic 35.0%
! AACCGTAGTAGTTTTCCTAA+TGG + chr4_3:50601891-50601910 Msa0645410:CDS 35.0%
TAAGACCAAAGATTTAGGGA+TGG + chr4_3:50602095-50602114 Msa0645410:intron 35.0%
TCATTCCATCCCTAAATCTT+TGG - chr4_3:50602103-50602122 None:intergenic 35.0%
! TGGAAGAGGACAAAGTATTT+GGG + chr4_3:50602217-50602236 Msa0645410:CDS 35.0%
AAATATGCTCATGAACATCC+AGG + chr4_3:50602242-50602261 Msa0645410:CDS 35.0%
AAACGTCATAGTTAAGCGAT+TGG - chr4_3:50602751-50602770 None:intergenic 35.0%
GACAAACGGAGTTAAGTTAT+GGG - chr4_3:50602812-50602831 None:intergenic 35.0%
TGACAAACGGAGTTAAGTTA+TGG - chr4_3:50602813-50602832 None:intergenic 35.0%
GTGGACCACATATAACAATA+GGG - chr4_3:50602863-50602882 None:intergenic 35.0%
GCATGAGAAAGAATAGGAAT+TGG + chr4_3:50603064-50603083 Msa0645410:intron 35.0%
! TAATCATGTTTGAGACGTGT+CGG - chr4_3:50603204-50603223 None:intergenic 35.0%
GTGGGACCAAAAAAAGTTAA+AGG + chr4_3:50603275-50603294 Msa0645410:intron 35.0%
GACTACTTTCAGTAACAACA+TGG - chr4_3:50603333-50603352 None:intergenic 35.0%
CATACAGATTCTCTATCTCT+TGG + chr4_3:50603714-50603733 Msa0645410:CDS 35.0%
AGTGCAGGTATAAATCAAGA+AGG + chr4_3:50603880-50603899 Msa0645410:CDS 35.0%
!! ATTGATGGAGGTATTGATAC+AGG + chr4_3:50604046-50604065 Msa0645410:intron 35.0%
GACGTCATAAAGTTCTATCA+TGG - chr4_3:50604265-50604284 None:intergenic 35.0%
AAACCATATATCCTCATGTG+GGG - chr4_3:50604305-50604324 None:intergenic 35.0%
GAAACCATATATCCTCATGT+GGG - chr4_3:50604306-50604325 None:intergenic 35.0%
TGAAACCATATATCCTCATG+TGG - chr4_3:50604307-50604326 None:intergenic 35.0%
TAACTCGAATTAATGGCATC+AGG + chr4_3:50604607-50604626 Msa0645410:intron 35.0%
GCTTCTCAAAATGGTTCAAT+AGG + chr4_3:50604629-50604648 Msa0645410:CDS 35.0%
!! TCTAACGTGTCATTTGAGAA+CGG - chr4_3:50604683-50604702 None:intergenic 35.0%
CTCTTGATTTCATGTTTGGA+TGG + chr4_3:50604723-50604742 Msa0645410:CDS 35.0%
TGAGTATCCTTGAAATATCC+AGG - chr4_3:50605068-50605087 None:intergenic 35.0%
TGCAGTTACTGCTATATATC+TGG + chr4_3:50605276-50605295 Msa0645410:intron 35.0%
GCAGTTACTGCTATATATCT+GGG + chr4_3:50605277-50605296 Msa0645410:intron 35.0%
CTTGGATAAACAAATAGCCA+AGG - chr4_3:50605385-50605404 None:intergenic 35.0%
TGGCTATTTGTTTATCCAAG+AGG + chr4_3:50605385-50605404 Msa0645410:CDS 35.0%
GGCTATTTGTTTATCCAAGA+GGG + chr4_3:50605386-50605405 Msa0645410:CDS 35.0%
!!! TGACTTTTTTGGTTAAGGCA+TGG + chr4_3:50606120-50606139 Msa0645410:intron 35.0%
TGCTGCCATTATTGGAACAA+AGG - chr4_3:50601832-50601851 None:intergenic 40.0%
!! TATGAAGGTGCTGCAAATGA+AGG + chr4_3:50602194-50602213 Msa0645410:CDS 40.0%
! GTGGAAGAGGACAAAGTATT+TGG + chr4_3:50602216-50602235 Msa0645410:CDS 40.0%
!! GTCTTGTTCTGGAAGTTAGT+AGG + chr4_3:50602453-50602472 Msa0645410:intron 40.0%
!! GTTCTGGAAGTTAGTAGGAA+AGG + chr4_3:50602458-50602477 Msa0645410:intron 40.0%
CTTAACTCCGTTTGTCAAGT+TGG + chr4_3:50602816-50602835 Msa0645410:intron 40.0%
GGTGGACCACATATAACAAT+AGG - chr4_3:50602864-50602883 None:intergenic 40.0%
CAAACTAATGAAGTCATCGG+TGG - chr4_3:50602882-50602901 None:intergenic 40.0%
TGCACTTAGGAGATGAGAAA+TGG - chr4_3:50602997-50603016 None:intergenic 40.0%
AATAGACAAGAGCTGCACTT+AGG - chr4_3:50603010-50603029 None:intergenic 40.0%
GTTTGAATTACAACTGAGGG+AGG + chr4_3:50603162-50603181 Msa0645410:intron 40.0%
! AGCTCTAACAAACATGCAGA+AGG + chr4_3:50603850-50603869 Msa0645410:intron 40.0%
! ATTTGTGACCCTTTTCCACT+GGG + chr4_3:50604078-50604097 Msa0645410:CDS 40.0%
AATGTAATTGACCCCACATG+AGG + chr4_3:50604291-50604310 Msa0645410:intron 40.0%
GAATTCCTTGAAGCATAGCT+CGG - chr4_3:50604389-50604408 None:intergenic 40.0%
GAGCTATGCTTCAAGGAATT+CGG + chr4_3:50604388-50604407 Msa0645410:intron 40.0%
ACACTCTTACAGTTTGGGTA+CGG + chr4_3:50604447-50604466 Msa0645410:CDS 40.0%
GCATCACCTTAAATTGTCAC+TGG + chr4_3:50604651-50604670 Msa0645410:CDS 40.0%
! CATGAAATCAAGAGCACGTT+CGG - chr4_3:50604717-50604736 None:intergenic 40.0%
CGTGCTCTTGATTTCATGTT+TGG + chr4_3:50604719-50604738 Msa0645410:CDS 40.0%
CACAATGTTCCGCAAAATCA+TGG + chr4_3:50604762-50604781 Msa0645410:intron 40.0%
! TGTCGTGATCCATGATTTTG+CGG - chr4_3:50604774-50604793 None:intergenic 40.0%
! GAGCAGTCTAAGATGCTTAT+TGG + chr4_3:50604960-50604979 Msa0645410:CDS 40.0%
AATAATGACACAAGCACACC+TGG + chr4_3:50605047-50605066 Msa0645410:CDS 40.0%
AAGCACACCTGGATATTTCA+AGG + chr4_3:50605058-50605077 Msa0645410:CDS 40.0%
AATAGAGTCTTACTCACCCT+TGG - chr4_3:50605257-50605276 None:intergenic 40.0%
CTGAGAAACGACACTTATGT+AGG + chr4_3:50605351-50605370 Msa0645410:intron 40.0%
!! TTAGTTATTGGCTTGGGATC+TGG + chr4_3:50605550-50605569 Msa0645410:intron 40.0%
!! TAGTTATTGGCTTGGGATCT+GGG + chr4_3:50605551-50605570 Msa0645410:intron 40.0%
! ATTCTCACCGGTGTCAATTT+AGG + chr4_3:50605604-50605623 Msa0645410:intron 40.0%
!! GACAATTTTGAATGGCTTGC+CGG + chr4_3:50606180-50606199 Msa0645410:CDS 40.0%
ACCAAAAGCTTTCAGCTCAT+TGG + chr4_3:50606259-50606278 Msa0645410:CDS 40.0%
! ACCAATGAGCTGAAAGCTTT+TGG - chr4_3:50606263-50606282 None:intergenic 40.0%
GTAACAGCTGCTGCCATTAT+TGG - chr4_3:50601840-50601859 None:intergenic 45.0%
! GTACGGTCAACAAGTGTTGT+AGG - chr4_3:50601864-50601883 None:intergenic 45.0%
TACAAAACGCACCTGGTAAG+AGG - chr4_3:50601940-50601959 None:intergenic 45.0%
TTAGGCGACAAATACCTGTC+AGG + chr4_3:50603112-50603131 Msa0645410:intron 45.0%
GCAGAAGGAAAAGTAAGTGC+AGG + chr4_3:50603865-50603884 Msa0645410:intron 45.0%
!! TGGAGGTATTGATACAGGTC+TGG + chr4_3:50604051-50604070 Msa0645410:intron 45.0%
CAGTGGAAAAGGGTCACAAA+TGG - chr4_3:50604079-50604098 None:intergenic 45.0%
! CATTTGTGACCCTTTTCCAC+TGG + chr4_3:50604077-50604096 Msa0645410:CDS 45.0%
TGACCCCACATGAGGATATA+TGG + chr4_3:50604299-50604318 Msa0645410:intron 45.0%
! GTAAGAGTGTGGCTCATTGA+GGG - chr4_3:50604437-50604456 None:intergenic 45.0%
! TGTAAGAGTGTGGCTCATTG+AGG - chr4_3:50604438-50604457 None:intergenic 45.0%
TGAGCCACACTCTTACAGTT+TGG + chr4_3:50604441-50604460 Msa0645410:CDS 45.0%
GAGCCACACTCTTACAGTTT+GGG + chr4_3:50604442-50604461 Msa0645410:CDS 45.0%
GTACCCAAACTGTAAGAGTG+TGG - chr4_3:50604448-50604467 None:intergenic 45.0%
TTTGGGTACGGAACCTTACA+TGG + chr4_3:50604459-50604478 Msa0645410:CDS 45.0%
TGGCATCAGGCTTCTCAAAA+TGG + chr4_3:50604620-50604639 Msa0645410:intron 45.0%
TTTATGCAGCCACTGACAAC+AGG + chr4_3:50604879-50604898 Msa0645410:CDS 45.0%
!! AGGAGAGTATCCATCAAGCA+TGG + chr4_3:50604899-50604918 Msa0645410:CDS 45.0%
ACGAAAGACACCATGCTTGA+TGG - chr4_3:50604912-50604931 None:intergenic 45.0%
ATCAAGCATGGTGTCTTTCG+TGG + chr4_3:50604911-50604930 Msa0645410:CDS 45.0%
TCAAGCATGGTGTCTTTCGT+GGG + chr4_3:50604912-50604931 Msa0645410:CDS 45.0%
AAGCATCTTAGACTGCTCTC+TGG - chr4_3:50604958-50604977 None:intergenic 45.0%
CCTCATAGGAGTTGTGATCA+AGG - chr4_3:50605114-50605133 None:intergenic 45.0%
CCTTGATCACAACTCCTATG+AGG + chr4_3:50605111-50605130 Msa0645410:intron 45.0%
! CTTTTGACAGCTGAGCGTAA+CGG + chr4_3:50605218-50605237 Msa0645410:intron 45.0%
! TTTTGACAGCTGAGCGTAAC+GGG + chr4_3:50605219-50605238 Msa0645410:intron 45.0%
TTACTCACCCTTGGTCCAAT+TGG - chr4_3:50605248-50605267 None:intergenic 45.0%
TTATGTAGGCTGCTTCACCT+TGG + chr4_3:50605365-50605384 Msa0645410:intron 45.0%
AGCAGTTCTTGAATCCCTCT+TGG - chr4_3:50605403-50605422 None:intergenic 45.0%
! TCACCGGTGTCAATTTAGGT+GGG + chr4_3:50605608-50605627 Msa0645410:intron 45.0%
TCCAACGCTATCACTTGAAG+AGG + chr4_3:50605926-50605945 Msa0645410:CDS 45.0%
!! ATCACTTGAAGAGGCACTCA+TGG + chr4_3:50605935-50605954 Msa0645410:CDS 45.0%
! AGGCATGGTGTGAAAGTACA+AGG + chr4_3:50606135-50606154 Msa0645410:intron 45.0%
TCCAAAACGTCTGGTGTATC+CGG - chr4_3:50606202-50606221 None:intergenic 45.0%
!! AAAAAAAAAAAAAAACTTAA+AGG - chr4_3:50602691-50602710 None:intergenic 5.0%
!! TAAAAAAAAAAAAAAAGATT+AGG - chr4_3:50605716-50605735 None:intergenic 5.0%
!!! AAATTTTAATAACTAAATTT+AGG + chr4_3:50605885-50605904 Msa0645410:intron 5.0%
TACTGCCTCATCCTCTTACC+AGG + chr4_3:50601926-50601945 Msa0645410:CDS 50.0%
!! GAAGGTGCTGCAAATGAAGG+TGG + chr4_3:50602197-50602216 Msa0645410:CDS 50.0%
GCTGCAAATGAAGGTGGAAG+AGG + chr4_3:50602203-50602222 Msa0645410:CDS 50.0%
TAGGTTATGTAGCGCCTGAC+AGG - chr4_3:50603129-50603148 None:intergenic 50.0%
GGGAAGATCCCAGTGGAAAA+GGG - chr4_3:50604089-50604108 None:intergenic 50.0%
TACTCGTCTTGTAGCGCTTG+GGG - chr4_3:50604109-50604128 None:intergenic 50.0%
GTACTCGTCTTGTAGCGCTT+GGG - chr4_3:50604110-50604129 None:intergenic 50.0%
TGTACTCGTCTTGTAGCGCT+TGG - chr4_3:50604111-50604130 None:intergenic 50.0%
CTATGCCGAGCTATGCTTCA+AGG + chr4_3:50604381-50604400 Msa0645410:intron 50.0%
TCGGAGACAGAGTGAAGCAT+TGG + chr4_3:50604407-50604426 Msa0645410:intron 50.0%
TGGTTGTGTGAGGCCATGTA+AGG - chr4_3:50604475-50604494 None:intergenic 50.0%
! AGCAAGGAGTTGGTTGTGTG+AGG - chr4_3:50604485-50604504 None:intergenic 50.0%
!! TCGGTGTTGCACCTGATGAT+TGG - chr4_3:50604536-50604555 None:intergenic 50.0%
GGATACTCTCCTGTTGTCAG+TGG - chr4_3:50604891-50604910 None:intergenic 50.0%
GACTGCTCTCTGGTGAACTT+TGG - chr4_3:50604948-50604967 None:intergenic 50.0%
! GAGCGTAACGGGATACCAAT+TGG + chr4_3:50605230-50605249 Msa0645410:CDS 50.0%
ACGGGATACCAATTGGACCA+AGG + chr4_3:50605237-50605256 Msa0645410:CDS 50.0%
CGGGATACCAATTGGACCAA+GGG + chr4_3:50605238-50605257 Msa0645410:CDS 50.0%
GTCTCAGGTTCGATTCTCAC+CGG + chr4_3:50605592-50605611 Msa0645410:intron 50.0%
! CTCACCGGTGTCAATTTAGG+TGG + chr4_3:50605607-50605626 Msa0645410:intron 50.0%
! GCCTCTTCAAGTGATAGCGT+TGG - chr4_3:50605930-50605949 None:intergenic 50.0%
ACGCACCTGGTAAGAGGATG+AGG - chr4_3:50601934-50601953 None:intergenic 55.0%
GGGGAAGATCCCAGTGGAAA+AGG - chr4_3:50604090-50604109 None:intergenic 55.0%
GCGCTACAAGACGAGTACAG+CGG + chr4_3:50604112-50604131 Msa0645410:CDS 55.0%
CAGCTGCATGAGCAAGGAGT+TGG - chr4_3:50604495-50604514 None:intergenic 55.0%
TGACAGCAGCTGCATGAGCA+AGG - chr4_3:50604501-50604520 None:intergenic 55.0%
GAATCGAACCTGAGACCTCG+AGG - chr4_3:50605588-50605607 None:intergenic 55.0%
CCGCCCACCTAAATTGACAC+CGG - chr4_3:50605614-50605633 None:intergenic 55.0%
! CCGGTGTCAATTTAGGTGGG+CGG + chr4_3:50605611-50605630 Msa0645410:intron 55.0%
! CGGTGTCAATTTAGGTGGGC+GGG + chr4_3:50605612-50605631 Msa0645410:intron 55.0%
! GCCGGATACACCAGACGTTT+TGG + chr4_3:50606198-50606217 Msa0645410:CDS 55.0%
TCGAACCTGAGACCTCGAGG+AGG - chr4_3:50605585-50605604 None:intergenic 60.0%
GCGCTTGGGGAAGATCCCAG+TGG - chr4_3:50604096-50604115 None:intergenic 65.0%
! TGGGAGTGTGCTCCTCCTCG+AGG + chr4_3:50605570-50605589 Msa0645410:intron 65.0%
GTGCTCCTCCTCGAGGTCTC+AGG + chr4_3:50605577-50605596 Msa0645410:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 50601768 50606308 50601768 ID=Msa0645410;Name=Msa0645410
chr4_3 mRNA 50601768 50606308 50601768 ID=Msa0645410-mRNA-1;Parent=Msa0645410;Name=Msa0645410-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|0.16|0.07|1|1|1|13|0|477
chr4_3 exon 50601768 50601947 50601768 ID=Msa0645410-mRNA-1:exon:12053;Parent=Msa0645410-mRNA-1
chr4_3 exon 50602194 50602263 50602194 ID=Msa0645410-mRNA-1:exon:12054;Parent=Msa0645410-mRNA-1
chr4_3 exon 50603412 50603470 50603412 ID=Msa0645410-mRNA-1:exon:12055;Parent=Msa0645410-mRNA-1
chr4_3 exon 50603677 50603752 50603677 ID=Msa0645410-mRNA-1:exon:12056;Parent=Msa0645410-mRNA-1
chr4_3 exon 50603869 50603946 50603869 ID=Msa0645410-mRNA-1:exon:12057;Parent=Msa0645410-mRNA-1
chr4_3 exon 50604068 50604155 50604068 ID=Msa0645410-mRNA-1:exon:12058;Parent=Msa0645410-mRNA-1
chr4_3 exon 50604417 50604543 50604417 ID=Msa0645410-mRNA-1:exon:12059;Parent=Msa0645410-mRNA-1
chr4_3 exon 50604629 50604744 50604629 ID=Msa0645410-mRNA-1:exon:12060;Parent=Msa0645410-mRNA-1
chr4_3 exon 50604878 50605101 50604878 ID=Msa0645410-mRNA-1:exon:12061;Parent=Msa0645410-mRNA-1
chr4_3 exon 50605228 50605259 50605228 ID=Msa0645410-mRNA-1:exon:12062;Parent=Msa0645410-mRNA-1
chr4_3 exon 50605373 50605475 50605373 ID=Msa0645410-mRNA-1:exon:12063;Parent=Msa0645410-mRNA-1
chr4_3 exon 50605907 50606015 50605907 ID=Msa0645410-mRNA-1:exon:12064;Parent=Msa0645410-mRNA-1
chr4_3 exon 50606137 50606308 50606137 ID=Msa0645410-mRNA-1:exon:12065;Parent=Msa0645410-mRNA-1
chr4_3 CDS 50601768 50601947 50601768 ID=Msa0645410-mRNA-1:cds;Parent=Msa0645410-mRNA-1
chr4_3 CDS 50602194 50602263 50602194 ID=Msa0645410-mRNA-1:cds;Parent=Msa0645410-mRNA-1
chr4_3 CDS 50603412 50603470 50603412 ID=Msa0645410-mRNA-1:cds;Parent=Msa0645410-mRNA-1
chr4_3 CDS 50603677 50603752 50603677 ID=Msa0645410-mRNA-1:cds;Parent=Msa0645410-mRNA-1
chr4_3 CDS 50603869 50603946 50603869 ID=Msa0645410-mRNA-1:cds;Parent=Msa0645410-mRNA-1
chr4_3 CDS 50604068 50604155 50604068 ID=Msa0645410-mRNA-1:cds;Parent=Msa0645410-mRNA-1
chr4_3 CDS 50604417 50604543 50604417 ID=Msa0645410-mRNA-1:cds;Parent=Msa0645410-mRNA-1
chr4_3 CDS 50604629 50604744 50604629 ID=Msa0645410-mRNA-1:cds;Parent=Msa0645410-mRNA-1
chr4_3 CDS 50604878 50605101 50604878 ID=Msa0645410-mRNA-1:cds;Parent=Msa0645410-mRNA-1
chr4_3 CDS 50605228 50605259 50605228 ID=Msa0645410-mRNA-1:cds;Parent=Msa0645410-mRNA-1
chr4_3 CDS 50605373 50605475 50605373 ID=Msa0645410-mRNA-1:cds;Parent=Msa0645410-mRNA-1
chr4_3 CDS 50605907 50606015 50605907 ID=Msa0645410-mRNA-1:cds;Parent=Msa0645410-mRNA-1
chr4_3 CDS 50606137 50606308 50606137 ID=Msa0645410-mRNA-1:cds;Parent=Msa0645410-mRNA-1
Gene Sequence

>Msa0645410

ATGGCATTCAATGGCCATCAATATTTCCTCTACTTTGGTGTCTTGAATTTTATCATTAGCTCCTTTGTTCCAATAATGGCAGCAGCTGTTACTCCTACAACACTTGTTGACCGTACTTCTCTAAACCGTAGTAGTTTTCCTAATGGTTTCATTTTTGGTACTGCCTCATCCTCTTACCAGTATGAAGGTGCTGCAAATGAAGGTGGAAGAGGACAAAGTATTTGGGATAAATATGCTCATGAACATCCAGAAAAAATATTGGATAGAAGCAATGGAGATGTAGCTGTTGATCAGTATCATCGTTATAAGGAAGATGTTGCAATCATGAAGTATATGAATACAGATGCATACAGATTCTCTATCTCTTGGCCAAGAATATTGCCAAAAGGAAAAGTAAGTGCAGGTATAAATCAAGAAGGAATCAAATATTACAACAATCTGATCAATGAGCTACTTGATAATGGTCTGGTACCATTTGTGACCCTTTTCCACTGGGATCTTCCCCAAGCGCTACAAGACGAGTACAGCGGCTTCTTACGCCCTAATATTATAGTGAAGCATTGGATTACCCTCAATGAGCCACACTCTTACAGTTTGGGTACGGAACCTTACATGGCCTCACACAACCAACTCCTTGCTCATGCAGCTGCTGTCAAACTTTATAGAACCAATCATCAGGCTTCTCAAAATGGTTCAATAGGCATCACCTTAAATTGTCACTGGTTTTTGCCGTTCTCAAATGACACGTTAGATCATCAAGCTGCCGAACGTGCTCTTGATTTCATGTTTGGATGGTTTATGCAGCCACTGACAACAGGAGAGTATCCATCAAGCATGGTGTCTTTCGTGGGAAATCGATTACCAAAGTTCACCAGAGAGCAGTCTAAGATGCTTATTGGTTCATTTGATTTTATTGGAATAAATTACTACACCTCCAATTATGCTTCCAATATACCACAATCAAATAATGACACAAGCACACCTGGATATTTCAAGGATACTCATGTCAATTTAACAACTGAGCGTAACGGGATACCAATTGGACCAAGGGCTGCTTCACCTTGGCTATTTGTTTATCCAAGAGGGATTCAAGAACTGCTGTTGTACACAAAGACAAAGTATAACAATCCTTTGATTTACATAACAGAAAATGGTATGGACGAGTTAAATGATCCAACGCTATCACTTGAAGAGGCACTCATGGATACTTACAGAATCAATTACTTTTATCGACATCTTTATTATATTTCATCTGCAATCAAGCATGGTGTGAAAGTACAAGGATATTTTGCATGGTCACTTTTGGACAATTTTGAATGGCTTGCCGGATACACCAGACGTTTTGGAATTAACTTTGTAGATTACAAAGATAATCTTAAAAGACACCAAAAGCTTTCAGCTCATTGGTTTAGAAATTTTCTTAAAAAGCAGTAG
Protein sequence

>Msa0645410

MAFNGHQYFLYFGVLNFIISSFVPIMAAAVTPTTLVDRTSLNRSSFPNGFIFGTASSSYQYEGAANEGGRGQSIWDKYAHEHPEKILDRSNGDVAVDQYHRYKEDVAIMKYMNTDAYRFSISWPRILPKGKVSAGINQEGIKYYNNLINELLDNGLVPFVTLFHWDLPQALQDEYSGFLRPNIIVKHWITLNEPHSYSLGTEPYMASHNQLLAHAAAVKLYRTNHQASQNGSIGITLNCHWFLPFSNDTLDHQAAERALDFMFGWFMQPLTTGEYPSSMVSFVGNRLPKFTREQSKMLIGSFDFIGINYYTSNYASNIPQSNNDTSTPGYFKDTHVNLTTERNGIPIGPRAASPWLFVYPRGIQELLLYTKTKYNNPLIYITENGMDELNDPTLSLEEALMDTYRINYFYRHLYYISSAIKHGVKVQGYFAWSLLDNFEWLAGYTRRFGINFVDYKDNLKRHQKLSAHWFRNFLKKQ*