AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0685000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0685000 MtrunA17_Chr4g0033261 96.154 494 19 0 1 494 1 494 0.0 999
Msa0685000 MtrunA17_Chr4g0033231 89.496 476 50 0 19 494 19 494 0.0 906
Msa0685000 MtrunA17_Chr2g0329201 69.185 503 126 3 17 493 8 507 0.0 721
Msa0685000 MtrunA17_Chr2g0329191 67.540 496 129 5 24 493 20 509 0.0 694
Msa0685000 MtrunA17_Chr6g0482071 65.116 516 147 5 8 494 3 514 0.0 682
Msa0685000 MtrunA17_Chr6g0482131 64.258 512 153 4 9 494 6 513 0.0 676
Msa0685000 MtrunA17_Chr4g0006111 68.050 482 140 6 23 493 47 525 0.0 675
Msa0685000 MtrunA17_Chr3g0088441 60.962 520 172 3 1 494 1 515 0.0 656
Msa0685000 MtrunA17_Chr4g0033311 60.769 520 174 3 1 494 1 516 0.0 655
Msa0685000 MtrunA17_Chr4g0033271 63.043 506 157 3 14 493 10 511 0.0 654
Msa0685000 MtrunA17_Chr4g0033341 60.777 515 170 4 9 493 31 543 0.0 654
Msa0685000 MtrunA17_Chr6g0482081 64.016 503 154 3 18 494 13 514 0.0 652
Msa0685000 MtrunA17_Chr4g0033401 63.116 507 153 3 17 491 4 508 0.0 649
Msa0685000 MtrunA17_Chr4g0033201 61.265 506 165 3 17 493 9 512 0.0 649
Msa0685000 MtrunA17_Chr2g0329321 61.850 519 141 5 1 493 1 488 0.0 646
Msa0685000 MtrunA17_Chr4g0033321 62.083 509 160 3 17 494 4 510 0.0 644
Msa0685000 MtrunA17_Chr4g0033331 62.575 497 158 2 21 491 19 513 0.0 644
Msa0685000 MtrunA17_Chr4g0033191 62.680 485 153 2 35 493 37 519 0.0 643
Msa0685000 MtrunA17_Chr4g0006131 65.773 485 153 8 18 492 19 500 0.0 634
Msa0685000 MtrunA17_Chr5g0430451 57.692 520 149 5 17 493 7 498 0.0 583
Msa0685000 MtrunA17_Chr4g0010501 58.125 480 169 3 39 493 22 494 0.0 578
Msa0685000 MtrunA17_Chr2g0301231 68.528 394 113 6 109 493 1 392 0.0 546
Msa0685000 MtrunA17_Chr3g0087261 49.315 511 224 5 9 493 8 509 1.16e-177 508
Msa0685000 MtrunA17_Chr3g0087271 49.805 512 225 4 7 493 6 510 5.83e-177 507
Msa0685000 MtrunA17_Chr4g0043091 49.505 505 212 7 23 492 6 502 2.75e-171 492
Msa0685000 MtrunA17_Chr4g0042171 48.805 502 219 5 26 492 1 499 1.69e-164 475
Msa0685000 MtrunA17_Chr3g0090131 48.723 470 218 6 40 492 20 483 4.15e-159 461
Msa0685000 MtrunA17_Chr6g0482941 44.808 520 253 7 1 494 2 513 8.35e-159 461
Msa0685000 MtrunA17_Chr3g0090111 48.614 469 217 7 41 492 19 480 1.61e-158 459
Msa0685000 MtrunA17_Chr6g0482951 45.155 516 245 8 6 494 9 513 1.87e-157 457
Msa0685000 MtrunA17_Chr4g0074561 46.512 473 224 4 46 491 40 510 3.37e-151 442
Msa0685000 MtrunA17_Chr2g0288071 45.759 507 237 7 18 494 45 543 4.24e-150 440
Msa0685000 MtrunA17_Chr4g0074551 47.182 479 215 8 46 494 55 525 1.64e-143 423
Msa0685000 MtrunA17_Chr2g0317821 44.583 480 229 7 41 491 32 503 6.19e-140 413
Msa0685000 MtrunA17_Chr2g0317841 44.748 476 234 6 41 491 32 503 7.20e-140 413
Msa0685000 MtrunA17_Chr2g0288021 41.732 508 256 8 17 494 13 510 2.48e-135 401
Msa0685000 MtrunA17_Chr7g0242691 41.968 498 246 8 25 494 14 496 1.80e-132 394
Msa0685000 MtrunA17_Chr4g0006971 42.916 487 226 9 40 494 26 492 1.77e-128 383
Msa0685000 MtrunA17_Chr7g0242681 41.701 482 236 9 41 494 38 502 1.76e-124 373
Msa0685000 MtrunA17_Chr5g0428831 41.286 482 232 8 42 492 28 489 2.73e-123 370
Msa0685000 MtrunA17_Chr1g0195611 41.667 480 232 8 42 492 28 488 4.79e-122 367
Msa0685000 MtrunA17_Chr3g0087201 53.869 336 120 4 185 493 17 344 6.59e-122 360
Msa0685000 MtrunA17_Chr7g0234091 42.918 473 234 6 42 493 76 533 3.45e-121 365
Msa0685000 MtrunA17_Chr7g0234111 43.220 472 230 7 42 491 76 531 8.30e-119 359
Msa0685000 MtrunA17_Chr7g0234131 40.592 473 242 6 42 491 76 532 5.88e-114 347
Msa0685000 MtrunA17_Chr5g0418871 51.839 299 115 4 60 332 2 297 5.79e-104 314
Msa0685000 MtrunA17_Chr1g0195641 43.188 389 180 6 43 403 29 404 3.58e-102 312
Msa0685000 MtrunA17_Chr7g0230651 41.600 375 187 5 87 441 17 379 6.61e-95 292
Msa0685000 MtrunA17_Chr3g0087191 69.863 146 44 0 39 184 20 165 1.37e-71 224
Msa0685000 MtrunA17_Chr7g0227561 41.065 263 110 3 42 285 47 283 1.81e-65 213
Msa0685000 MtrunA17_Chr7g0236731 44.958 238 104 3 130 340 28 265 5.97e-55 185
Msa0685000 MtrunA17_Chr4g0068871 27.182 401 227 12 105 491 183 532 1.12e-27 116
Msa0685000 MtrunA17_Chr5g0418861 52.222 90 42 1 403 491 1 90 8.91e-26 100
Msa0685000 MtrunA17_Chr8g0352881 43.299 97 55 0 242 338 58 154 4.44e-20 87.0
Msa0685000 MtrunA17_Chr8g0352881 51.852 54 25 1 50 103 7 59 3.49e-12 64.7
Msa0685000 MtrunA17_Chr2g0301221 71.930 57 15 1 26 81 49 105 2.37e-15 72.4
Msa0685000 MtrunA17_Chr7g0227591 51.923 52 25 0 440 491 14 65 4.25e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0685000 AT2G44450.1 53.542 480 190 4 41 494 31 503 0.0 535
Msa0685000 AT5G44640.1 51.556 514 205 7 11 494 5 504 0.0 534
Msa0685000 AT5G42260.1 51.581 506 205 7 10 487 4 497 0.0 527
Msa0685000 AT3G60130.1 53.112 482 192 5 38 492 27 501 0.0 521
Msa0685000 AT2G44480.3 51.025 488 212 2 29 491 54 539 0.0 521
Msa0685000 AT2G44480.1 51.025 488 212 2 29 491 23 508 0.0 521
Msa0685000 AT2G25630.1 49.606 508 200 7 11 491 5 483 1.28e-172 495
Msa0685000 AT1G26560.1 51.255 478 203 4 41 491 32 506 9.91e-170 488
Msa0685000 AT5G24550.1 48.627 510 225 8 14 493 9 511 2.02e-168 486
Msa0685000 AT5G54570.1 49.068 483 217 3 36 491 25 505 9.56e-166 479
Msa0685000 AT5G24540.1 48.824 510 224 8 14 493 9 511 7.68e-163 472
Msa0685000 AT2G44490.1 48.163 490 220 7 34 492 7 493 5.47e-162 471
Msa0685000 AT3G60120.1 49.795 488 206 9 38 493 11 491 6.15e-162 470
Msa0685000 AT5G36890.2 49.174 484 225 5 26 492 1 480 1.13e-160 464
Msa0685000 AT5G36890.1 49.174 484 225 5 26 492 1 480 1.34e-160 464
Msa0685000 AT3G60140.1 45.508 512 237 7 11 493 6 504 1.88e-160 467
Msa0685000 AT3G18080.1 48.017 479 217 7 40 493 38 509 9.22e-157 456
Msa0685000 AT1G51470.1 48.218 477 215 5 40 493 44 511 1.47e-156 455
Msa0685000 AT1G47600.1 47.379 477 219 5 40 493 44 511 9.34e-156 453
Msa0685000 AT1G47600.2 47.379 477 218 5 40 493 44 510 1.43e-155 452
Msa0685000 AT2G44470.3 44.401 509 246 8 14 493 7 507 7.20e-153 448
Msa0685000 AT3G18070.3 46.653 478 215 8 41 493 30 492 1.16e-151 442
Msa0685000 AT3G60130.2 52.278 417 165 5 103 492 40 449 1.37e-151 441
Msa0685000 AT3G18070.1 46.862 478 220 8 41 493 30 498 2.61e-151 441
Msa0685000 AT2G44460.1 45.793 511 239 9 10 491 3 504 4.04e-150 441
Msa0685000 AT2G44480.2 49.265 408 180 2 109 491 1 406 4.39e-148 430
Msa0685000 AT5G54570.3 47.786 429 196 2 36 438 25 451 3.73e-143 419
Msa0685000 AT3G60130.3 50.839 417 160 6 103 492 40 438 1.60e-142 417
Msa0685000 AT1G61820.1 43.418 509 252 8 12 493 7 506 2.03e-142 419
Msa0685000 AT5G25980.2 43.126 531 255 13 1 492 1 523 2.66e-142 420
Msa0685000 AT1G75940.1 45.527 503 231 10 25 491 22 517 5.80e-142 419
Msa0685000 AT3G03640.1 41.279 516 265 7 9 493 6 514 1.78e-141 417
Msa0685000 AT1G66280.1 43.487 522 252 11 1 491 1 510 3.86e-141 416
Msa0685000 AT3G09260.1 43.983 482 237 7 41 493 35 512 2.72e-140 414
Msa0685000 AT2G32860.2 42.975 484 245 5 40 494 93 574 3.64e-140 417
Msa0685000 AT2G32860.1 42.126 508 252 7 26 494 69 573 7.38e-140 416
Msa0685000 AT5G26000.1 44.890 499 220 12 32 491 29 511 1.04e-139 413
Msa0685000 AT3G21370.1 44.668 497 231 10 30 492 26 512 5.71e-139 410
Msa0685000 AT5G28510.1 42.802 521 251 11 9 491 8 519 1.64e-138 410
Msa0685000 AT1G66270.1 44.000 500 238 9 30 493 19 512 5.70e-138 408
Msa0685000 AT5G24540.2 49.533 428 184 6 39 437 30 454 2.20e-137 404
Msa0685000 AT1G52400.3 43.888 499 236 10 30 494 30 518 1.14e-136 405
Msa0685000 AT1G52400.1 43.888 499 236 10 30 494 30 518 1.14e-136 405
Msa0685000 AT1G66270.2 43.800 500 237 10 30 493 19 510 9.35e-135 400
Msa0685000 AT1G02850.2 43.443 488 223 9 36 492 21 486 7.48e-134 397
Msa0685000 AT5G54570.2 47.805 410 185 3 109 491 1 408 1.51e-131 389
Msa0685000 AT1G61810.1 43.542 480 241 7 41 493 33 509 3.56e-131 390
Msa0685000 AT4G21760.2 41.584 505 259 7 15 491 25 521 4.97e-131 391
Msa0685000 AT2G44470.1 43.584 452 219 7 14 437 7 450 1.97e-130 386
Msa0685000 AT1G02850.5 41.683 511 222 10 36 492 21 509 2.20e-129 386
Msa0685000 AT2G44450.2 50.418 359 145 4 162 494 1 352 1.21e-128 378
Msa0685000 AT3G60140.2 45.000 420 190 6 11 402 6 412 1.72e-128 381
Msa0685000 AT1G02850.4 41.602 512 222 11 36 492 21 510 1.08e-127 382
Msa0685000 AT4G27830.1 41.235 502 244 9 18 491 6 484 2.02e-127 380
Msa0685000 AT1G61810.3 43.683 467 233 7 41 480 33 496 5.17e-126 378
Msa0685000 AT5G25980.3 42.947 475 226 11 1 438 1 467 1.13e-125 375
Msa0685000 AT5G25980.1 42.947 475 226 11 1 438 1 467 1.13e-125 375
Msa0685000 AT4G21760.1 40.792 505 253 8 15 491 25 511 2.99e-125 376
Msa0685000 AT5G26000.2 44.820 444 192 10 32 438 29 456 7.22e-123 367
Msa0685000 AT1G02850.1 41.598 488 205 10 36 492 21 459 1.61e-121 364
Msa0685000 AT4G27820.1 41.788 481 231 8 40 491 22 482 7.97e-121 363
Msa0685000 AT3G62750.1 39.796 490 253 9 22 491 6 473 5.66e-120 361
Msa0685000 AT3G62750.2 39.796 490 253 9 22 491 32 499 8.67e-120 361
Msa0685000 AT2G44480.4 53.376 311 120 1 29 314 54 364 8.37e-118 352
Msa0685000 AT4G22100.1 39.506 486 249 9 34 492 16 483 2.70e-116 352
Msa0685000 AT1G60090.2 37.500 480 256 7 41 494 24 485 3.59e-113 344
Msa0685000 AT3G62740.1 37.475 491 262 9 25 491 9 478 3.90e-113 343
Msa0685000 AT1G52400.2 42.404 441 211 9 30 437 30 460 3.19e-111 337
Msa0685000 AT5G48375.1 42.022 445 190 11 25 456 22 411 4.65e-111 336
Msa0685000 AT1G45191.4 38.462 481 244 9 42 494 30 486 5.27e-111 338
Msa0685000 AT1G45191.6 38.462 481 255 8 42 494 30 497 3.20e-110 337
Msa0685000 AT1G60090.1 36.420 486 260 8 41 494 24 492 3.73e-110 336
Msa0685000 AT2G44470.2 43.003 393 188 7 14 378 7 391 4.22e-110 332
Msa0685000 AT1G45191.2 37.787 479 256 7 42 494 30 492 2.38e-109 334
Msa0685000 AT1G45191.1 37.910 488 260 8 42 494 30 509 6.93e-109 333
Msa0685000 AT1G61810.5 42.233 412 208 7 109 493 1 409 5.26e-104 317
Msa0685000 AT1G02850.3 41.905 420 175 8 36 402 21 424 1.37e-103 317
Msa0685000 AT3G62750.4 38.952 439 227 8 21 440 31 447 3.37e-103 317
Msa0685000 AT1G61810.4 43.188 389 192 6 41 402 33 419 6.09e-102 312
Msa0685000 AT4G27830.2 41.119 411 199 7 109 491 1 396 2.22e-100 308
Msa0685000 AT3G18070.4 45.322 342 156 6 41 357 30 365 1.45e-98 302
Msa0685000 AT3G62740.2 37.385 436 229 8 25 437 9 423 8.43e-98 301
Msa0685000 AT2G44460.2 45.152 361 166 6 160 491 1 358 5.75e-95 295
Msa0685000 AT4G27820.2 40.534 412 201 7 109 491 1 397 8.49e-95 294
Msa0685000 AT4G27820.3 40.534 412 201 7 109 491 1 397 8.49e-95 294
Msa0685000 AT2G44480.5 48.727 275 139 1 217 491 30 302 2.54e-94 289
Msa0685000 AT3G62750.7 42.169 332 166 4 22 336 6 328 1.02e-93 289
Msa0685000 AT3G62750.8 42.169 332 166 4 22 336 6 328 1.02e-93 289
Msa0685000 AT3G18070.2 44.578 332 155 5 187 493 94 421 4.60e-93 289
Msa0685000 AT3G18070.2 54.688 64 28 1 40 103 29 91 4.89e-15 77.4
Msa0685000 AT3G62750.3 42.169 332 166 4 22 336 32 354 6.43e-93 289
Msa0685000 AT1G60270.1 41.056 341 170 3 41 356 24 358 4.22e-92 285
Msa0685000 AT1G61820.3 41.530 366 185 6 155 493 4 367 1.61e-91 284
Msa0685000 AT3G62750.6 38.710 403 212 7 109 491 1 388 2.52e-91 285
Msa0685000 AT3G62750.5 38.710 403 212 7 109 491 1 388 2.52e-91 285
Msa0685000 AT1G51490.1 45.115 348 152 9 48 369 27 361 4.86e-91 284
Msa0685000 AT3G18070.7 48.014 277 140 3 217 493 37 309 7.94e-89 275
Msa0685000 AT1G45191.5 38.617 347 181 3 42 363 30 369 1.00e-84 267
Msa0685000 AT1G45191.3 38.122 362 190 4 42 376 30 384 2.67e-84 266
Msa0685000 AT1G61810.2 46.097 269 119 3 41 284 33 300 4.79e-79 249
Msa0685000 AT1G60270.2 39.560 273 138 2 109 356 1 271 1.19e-64 211
Msa0685000 AT3G18070.5 41.322 242 116 3 187 403 94 334 6.38e-58 196
Msa0685000 AT3G18070.5 54.688 64 28 1 40 103 29 91 2.82e-15 77.4
Msa0685000 AT3G18070.6 45.455 187 101 1 217 403 37 222 6.47e-54 181
Msa0685000 AT5G16580.1 34.859 284 152 5 187 448 27 299 1.65e-48 169
Msa0685000 AT3G06510.1 26.536 407 226 17 104 492 159 510 1.40e-25 110
Msa0685000 AT3G06510.2 26.824 425 236 19 104 492 159 544 4.20e-25 109

Find 100 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGCAACTTACATAATATT+AGG 0.188638 4_4:+48820900 None:intergenic
GTGGAAGAGGACAAAGTATT+TGG 0.192835 4_4:-48822551 Msa0685000:CDS
TGTAATATTTGATTCCTTCT+TGG 0.196303 4_4:+48821102 None:intergenic
GAGCAATCTAAGATGCTTAT+TGG 0.212349 4_4:-48820135 Msa0685000:CDS
TTGATTTACATAACAGAAAA+TGG 0.216843 4_4:-48819639 Msa0685000:intron
GTTATACTTGGCAATATTCT+TGG 0.259733 4_4:+48821250 None:intergenic
TGGCATCAGGCTTCTCAAAA+TGG 0.265050 4_4:-48820425 Msa0685000:intron
TGGTATATTGGAAGCATAAT+TGG 0.270855 4_4:+48820077 None:intergenic
CGTGCTCTTGATTTCATGTT+TGG 0.285929 4_4:-48820326 Msa0685000:CDS
GTAACAGATGCTGCCATTAT+TGG 0.291365 4_4:+48822975 None:intergenic
ACCAATGAGCTGAAAGCTTT+TGG 0.307343 4_4:+48818899 None:intergenic
ATTATTTGATTGTGGTATAT+TGG 0.312657 4_4:+48820065 None:intergenic
TAGCTCCTTTGTTCCAATAA+TGG 0.326020 4_4:-48822988 Msa0685000:CDS
GCCGGATACACCAGACGTTT+TGG 0.335510 4_4:-48818961 Msa0685000:CDS
ATGCAACTTACATAATATTA+GGG 0.338145 4_4:+48820901 None:intergenic
ATACTTGTCTTGTAAAGCTT+GGG 0.359924 4_4:+48820938 None:intergenic
ATCAATGAACTACTTGATAA+TGG 0.365882 4_4:-48821071 Msa0685000:intron
TGAGCCACACTCTTACAGTT+TGG 0.367434 4_4:-48820604 Msa0685000:CDS
ACCAAAAGCTTTCAGCTCAT+TGG 0.373860 4_4:-48818900 Msa0685000:CDS
GCTTCTCAAAATGGTTCAAT+TGG 0.377536 4_4:-48820416 Msa0685000:CDS
TGATCAATATCATCGTTATA+AGG 0.390170 4_4:-48821603 Msa0685000:intron
TCCAAAACGTCTGGTGTATC+CGG 0.394369 4_4:+48818960 None:intergenic
ACGAAAGACACCATGCTTGA+TGG 0.404375 4_4:+48820186 None:intergenic
TATCAAGTAGTTCATTGATC+AGG 0.412549 4_4:+48821075 None:intergenic
GAGCCACACTCTTACAGTTT+GGG 0.414537 4_4:-48820603 Msa0685000:CDS
TGGAAGAGGACAAAGTATTT+GGG 0.414779 4_4:-48822550 Msa0685000:CDS
TCGGTGTTGCACCTGATGAT+TGG 0.418117 4_4:+48820512 None:intergenic
GGGGAAGATCCCAGTGGAAA+AGG 0.421057 4_4:+48820958 None:intergenic
TTACTCACCCTTGGTCCAAT+TGG 0.424891 4_4:+48819850 None:intergenic
TACAAAACGCACCTGGTAAG+AGG 0.428857 4_4:+48822875 None:intergenic
GAGCTATGCTTCAAGGAATT+CGG 0.432941 4_4:-48820657 Msa0685000:CDS
TATACTTGTCTTGTAAAGCT+TGG 0.437001 4_4:+48820937 None:intergenic
GATTGCTCTCTGGTGAACTT+TGG 0.452453 4_4:+48820150 None:intergenic
CTATGCCGAGCTATGCTTCA+AGG 0.457568 4_4:-48820664 Msa0685000:CDS
GAGCGTAACGGGATACCAAT+TGG 0.457796 4_4:-48819865 Msa0685000:CDS
TTTATGCAGCCACTGACAAC+AGG 0.464763 4_4:-48820216 Msa0685000:CDS
AGCAGTTCTTGAATCCCTCT+TGG 0.465436 4_4:+48819693 None:intergenic
AAAGTTAATTCCAAAACGTC+TGG 0.470833 4_4:+48818951 None:intergenic
CATCAATATCTCCTCTACTT+TGG 0.470993 4_4:-48823029 Msa0685000:CDS
ATCACTTGAAGAGGCGCTCA+TGG 0.476692 4_4:-48819224 Msa0685000:CDS
ATCAAGCATGGTGTCTTTCG+TGG 0.490440 4_4:-48820184 Msa0685000:CDS
CATACAGATTCTCCATCTCT+TGG 0.492132 4_4:-48821273 Msa0685000:CDS
CACAAGGGCATGGCATTCAA+TGG 0.496087 4_4:-48823053 None:intergenic
GGCTATTTGTTTATCCAAGA+GGG 0.499794 4_4:-48819707 Msa0685000:CDS
TCGGAGACAGAGTGAAGCAT+TGG 0.500114 4_4:-48820638 Msa0685000:CDS
GCATCACCTTAAATTGTCAC+TGG 0.501551 4_4:-48820394 Msa0685000:CDS
AAAATATTGGATGGAAGCAA+TGG 0.501970 4_4:-48821638 Msa0685000:CDS
GTACGGTCCACAAGTGTTGT+AGG 0.503023 4_4:+48822951 None:intergenic
CAAAGTAGAGGAGATATTGA+TGG 0.506004 4_4:+48823030 None:intergenic
TACTGCCTCATCCTCTTACC+AGG 0.507287 4_4:-48822886 Msa0685000:intron
TGAGTATCCTTGAAATATCC+AGG 0.513235 4_4:+48820030 None:intergenic
GGGAAGATCCCAGTGGAAAA+GGG 0.516334 4_4:+48820959 None:intergenic
GTAAGAGTGTGGCTCATTGA+GGG 0.516603 4_4:+48820611 None:intergenic
CAATATTCTTGGCCAAGAGA+TGG 0.516800 4_4:+48821261 None:intergenic
CTCTTGATTTCATGTTTGGA+TGG 0.519547 4_4:-48820322 Msa0685000:intron
ACGCACCTGGTAAGAGGATG+AGG 0.521862 4_4:+48822881 None:intergenic
AATAATGACACAAGCACACC+TGG 0.522457 4_4:-48820048 Msa0685000:CDS
GCTGCAAATGAAGGTGGAAG+AGG 0.523453 4_4:-48822564 Msa0685000:CDS
AAGCATCTTAGATTGCTCTC+TGG 0.523983 4_4:+48820140 None:intergenic
AACCTTTGGGAAAACTACTA+CGG 0.526702 4_4:+48822919 None:intergenic
TGGTTGTGTGAGGCCATGTA+AGG 0.527832 4_4:+48820573 None:intergenic
AAGCACACCTGGATATTTCA+AGG 0.530555 4_4:-48820037 Msa0685000:CDS
CAGCTGCATGAGCAAGGAGT+TGG 0.531565 4_4:+48820553 None:intergenic
ACTTTATAGAACCAATCATC+AGG 0.533812 4_4:-48820523 Msa0685000:intron
TGTAAGAGTGTGGCTCATTG+AGG 0.538006 4_4:+48820610 None:intergenic
GCAGAAGGAAAAGTAAATGA+AGG 0.542081 4_4:-48821131 Msa0685000:intron
TTTGGGTACGGAGCCTTACA+TGG 0.542587 4_4:-48820586 Msa0685000:CDS
CAGTGGAAAAGGGTCACAAA+TGG 0.545800 4_4:+48820969 None:intergenic
CTTGTGTCATTATTTGATTG+TGG 0.555807 4_4:+48820057 None:intergenic
TGACAGCAGCTGCATGAGCA+AGG 0.559687 4_4:+48820547 None:intergenic
AAATATGCTCATGAACATCC+AGG 0.571960 4_4:-48822525 Msa0685000:intron
AATGAAGGTATAAACCAAGA+AGG 0.573462 4_4:-48821116 Msa0685000:CDS
TACTTGTCTTGTAAAGCTTG+GGG 0.576901 4_4:+48820939 None:intergenic
TGCTGCCATTATTGGAACAA+AGG 0.580516 4_4:+48822983 None:intergenic
GTGTTGATAATATAGTATGA+AGG 0.581970 4_4:-48822588 Msa0685000:intron
AGGCATGGTGTGAAAGTACA+AGG 0.584642 4_4:-48819024 Msa0685000:CDS
TGTTACTCCTACAACACTTG+TGG 0.588446 4_4:-48822958 Msa0685000:CDS
TATATTGGAAGCATAATTGG+AGG 0.589479 4_4:+48820080 None:intergenic
TATGAAGGTGCTGCAAATGA+AGG 0.592608 4_4:-48822573 Msa0685000:CDS
TCCAACGCTATCACTTGAAG+AGG 0.593045 4_4:-48819233 Msa0685000:CDS
TGGCTATTTGTTTATCCAAG+AGG 0.593511 4_4:-48819708 Msa0685000:CDS
ACACTCTTACAGTTTGGGTA+CGG 0.597858 4_4:-48820598 Msa0685000:CDS
TCAAGCATGGTGTCTTTCGT+GGG 0.598348 4_4:-48820183 Msa0685000:CDS
GGATACTCTCCTGTTGTCAG+TGG 0.600363 4_4:+48820207 None:intergenic
GAATTCCTTGAAGCATAGCT+CGG 0.603333 4_4:+48820659 None:intergenic
CGGGATACCAATTGGACCAA+GGG 0.607534 4_4:-48819857 Msa0685000:intron
CTTGGATAAACAAATAGCCA+AGG 0.631378 4_4:+48819711 None:intergenic
GCCTCTTCAAGTGATAGCGT+TGG 0.633794 4_4:+48819232 None:intergenic
ATGTACAGGCTGCTTCACCT+TGG 0.644607 4_4:-48819728 Msa0685000:intron
AATTTAAGACACCAAAGTAG+AGG 0.647935 4_4:+48823018 None:intergenic
TACTACGGTTTAGAGAAGTA+CGG 0.651396 4_4:+48822934 None:intergenic
AGGAGAGTATCCATCAAGCA+TGG 0.653040 4_4:-48820196 Msa0685000:CDS
GAAGGTGCTGCAAATGAAGG+TGG 0.659790 4_4:-48822570 Msa0685000:CDS
AGCAAGGAGTTGGTTGTGTG+AGG 0.661595 4_4:+48820563 None:intergenic
ACGGGATACCAATTGGACCA+AGG 0.664320 4_4:-48819858 Msa0685000:CDS
CACGTTAGATCATCAAGCTG+CGG 0.666746 4_4:-48820351 Msa0685000:CDS
GCTTTACAAGACAAGTATAG+CGG 0.668281 4_4:-48820933 Msa0685000:CDS
GTACCCAAACTGTAAGAGTG+TGG 0.675082 4_4:+48820600 None:intergenic
TCTAACGTGTCATTTGAGAA+CGG 0.686339 4_4:+48820365 None:intergenic
AAGCTTGGGGAAGATCCCAG+TGG 0.742534 4_4:+48820952 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTGATATAATTAAAGAA+AGG + chr4_4:48819441-48819460 None:intergenic 10.0%
!! TTTCTTTAATTATATCAATT+TGG - chr4_4:48819440-48819459 Msa0685000:intron 10.0%
!!! AAAATATTTTACATCAAATT+AGG + chr4_4:48822011-48822030 None:intergenic 10.0%
!! TTATATTTATCTCTAAACTT+TGG + chr4_4:48819111-48819130 None:intergenic 15.0%
!! TTGAACAAAATTTAATACTT+AGG - chr4_4:48819236-48819255 Msa0685000:CDS 15.0%
!! TGAACAAAATTTAATACTTA+GGG - chr4_4:48819237-48819256 Msa0685000:CDS 15.0%
!!! CAAGTTATTTTATTTTTGAA+GGG + chr4_4:48819490-48819509 None:intergenic 15.0%
!!! TCAAGTTATTTTATTTTTGA+AGG + chr4_4:48819491-48819510 None:intergenic 15.0%
!! ACTTGAATGAAAAATAAAAT+AGG - chr4_4:48819505-48819524 Msa0685000:intron 15.0%
!!! CTATTTATGAAGTTTTATAT+TGG - chr4_4:48819777-48819796 Msa0685000:intron 15.0%
!! TAAAACAAAACAAAATAATG+AGG + chr4_4:48819929-48819948 None:intergenic 15.0%
!!! ACATATATAGTAATTTGTTT+GGG + chr4_4:48820152-48820171 None:intergenic 15.0%
!! AAACAAATTACTATATATGT+GGG - chr4_4:48820152-48820171 Msa0685000:CDS 15.0%
!!! TATATTCCTTTAACTTTTTT+TGG + chr4_4:48820179-48820198 None:intergenic 15.0%
!! ATTTCATACAGAAAAAATAT+TGG - chr4_4:48820266-48820285 Msa0685000:intron 15.0%
!! AAGTTGTACAAATATTATAA+TGG + chr4_4:48820422-48820441 None:intergenic 15.0%
!!! AATTTTTTGTCTTGTATATA+TGG - chr4_4:48820561-48820580 Msa0685000:CDS 15.0%
!!! AACCATTTTAACATTTTAAT+AGG - chr4_4:48820585-48820604 Msa0685000:CDS 15.0%
!! TTCCTATTAAAATGTTAAAA+TGG + chr4_4:48820590-48820609 None:intergenic 15.0%
!!! CAAATTGATTTTTTTATCAA+TGG + chr4_4:48821977-48821996 None:intergenic 15.0%
!!! ATATATCTAAAGTTAGTTAT+TGG - chr4_4:48822362-48822381 Msa0685000:intron 15.0%
!! ATCTCTTAAAAAAAAAGATT+AGG + chr4_4:48822541-48822560 None:intergenic 15.0%
!!! AATCTTTTTTTTTAAGAGAT+AGG - chr4_4:48822541-48822560 Msa0685000:CDS 15.0%
!! TTAATAACTAAATTTAGGTA+TGG - chr4_4:48822648-48822667 Msa0685000:intron 15.0%
!!! ATTATTATTTCTGACTTTTT+TGG - chr4_4:48822867-48822886 Msa0685000:intron 15.0%
!! AAGAAAAAAAGAAATATACC+TGG + chr4_4:48819413-48819432 None:intergenic 20.0%
!!! ATGAGTGATATTTTATGTAT+TGG - chr4_4:48819560-48819579 Msa0685000:intron 20.0%
!! AAAACTTCATAAATAGTTGA+GGG + chr4_4:48819773-48819792 None:intergenic 20.0%
!! TAAAACTTCATAAATAGTTG+AGG + chr4_4:48819774-48819793 None:intergenic 20.0%
!! GTGTACAAAATTAGATATAA+TGG + chr4_4:48819980-48819999 None:intergenic 20.0%
!! TAATTCGAACCTTAATTAAA+AGG + chr4_4:48820051-48820070 None:intergenic 20.0%
!!! CACATATATAGTAATTTGTT+TGG + chr4_4:48820153-48820172 None:intergenic 20.0%
!! CAAACAAATTACTATATATG+TGG - chr4_4:48820151-48820170 Msa0685000:CDS 20.0%
!!! ACAAATATTATAATGGATGA+TGG + chr4_4:48820415-48820434 None:intergenic 20.0%
!!! AATTCAACATTTGTTATACT+TGG + chr4_4:48820682-48820701 None:intergenic 20.0%
!! ATGCAACTTACATAATATTA+GGG + chr4_4:48821019-48821038 None:intergenic 20.0%
!! AATCATAACAAATACAAGAA+AGG + chr4_4:48821442-48821461 None:intergenic 20.0%
!!! TTGAGTTAGTTAAAGTTTTT+CGG + chr4_4:48821467-48821486 None:intergenic 20.0%
!! CTTTAACTAACTCAAATTAA+TGG - chr4_4:48821472-48821491 Msa0685000:intron 20.0%
!!! ATTGGTTCATTTGATTTTAT+TGG - chr4_4:48821800-48821819 Msa0685000:intron 20.0%
!! ATTATTTGATTGTGGTATAT+TGG + chr4_4:48821855-48821874 None:intergenic 20.0%
!!! TTTTCTGTTATGTAAATCAA+AGG + chr4_4:48822278-48822297 None:intergenic 20.0%
!! TTGATTTACATAACAGAAAA+TGG - chr4_4:48822278-48822297 Msa0685000:intron 20.0%
!! TGCATATTGTAAAAATCAAA+TGG + chr4_4:48822321-48822340 None:intergenic 20.0%
!!! ATTATACAATTGATTTGACT+AGG + chr4_4:48822617-48822636 None:intergenic 20.0%
!! TAAAATAAGCAAACTAAGTA+AGG + chr4_4:48822836-48822855 None:intergenic 20.0%
! CAAAATTTAATACTTAGGGA+TGG - chr4_4:48819241-48819260 Msa0685000:CDS 25.0%
! GATATGGCTAATATTATTGA+AGG - chr4_4:48819267-48819286 Msa0685000:intron 25.0%
! ATTATTGAAGGCTTAAGAAT+TGG - chr4_4:48819279-48819298 Msa0685000:intron 25.0%
!!! GTGTTGATAATATAGTATGA+AGG - chr4_4:48819329-48819348 Msa0685000:intron 25.0%
! ATAAAGTATAAAGAGGCTAA+AGG + chr4_4:48819542-48819561 None:intergenic 25.0%
! ATCACTCATAAAGTATAAAG+AGG + chr4_4:48819549-48819568 None:intergenic 25.0%
! CTTATGTAGCATACTATATA+TGG - chr4_4:48819643-48819662 Msa0685000:CDS 25.0%
! TGTAGTCATAATACTTATGT+TGG - chr4_4:48819682-48819701 Msa0685000:CDS 25.0%
! TAACTAACGAAAGTAAGTTA+TGG + chr4_4:48819834-48819853 None:intergenic 25.0%
!! TAATCATATTTGAGATGTGT+CGG + chr4_4:48820103-48820122 None:intergenic 25.0%
! TCAAATATGATTATCTCGAA+TGG - chr4_4:48820110-48820129 Msa0685000:CDS 25.0%
! TACTGAAAGTAATTGGTTAA+TGG - chr4_4:48820239-48820258 Msa0685000:intron 25.0%
! CATACAGAAAAAATATTGGA+TGG - chr4_4:48820270-48820289 Msa0685000:intron 25.0%
! TGATCAATATCATCGTTATA+AGG - chr4_4:48820314-48820333 Msa0685000:intron 25.0%
!! AGAATGCTTTTATATCGAAT+TGG + chr4_4:48820345-48820364 None:intergenic 25.0%
!!! TTATAATGGATGATGGATTT+TGG + chr4_4:48820408-48820427 None:intergenic 25.0%
! ATGAAACTTTAGGTGAATAA+AGG + chr4_4:48820714-48820733 None:intergenic 25.0%
! TGTAATATTTGATTCCTTCT+TGG + chr4_4:48820818-48820837 None:intergenic 25.0%
! ATCAATGAACTACTTGATAA+TGG - chr4_4:48820846-48820865 Msa0685000:intron 25.0%
! ATCATAGTAATGTAAATCGA+TGG - chr4_4:48820903-48820922 Msa0685000:intron 25.0%
! ATCGATGGAATTATTGATAT+AGG - chr4_4:48820918-48820937 Msa0685000:CDS 25.0%
! CATGCAACTTACATAATATT+AGG + chr4_4:48821020-48821039 None:intergenic 25.0%
! CTAATATTATGTAAGTTGCA+TGG - chr4_4:48821018-48821037 Msa0685000:intron 25.0%
!!! ATGTTATTTCAATTCCTCAT+AGG + chr4_4:48821950-48821969 None:intergenic 25.0%
!! AATTTTCATTCTTAAGAGAG+AGG - chr4_4:48822512-48822531 Msa0685000:intron 25.0%
! TATTGAGGGTAAAAAAGATT+AGG + chr4_4:48822570-48822589 None:intergenic 25.0%
! AGAATCAAACAATGTATTGA+GGG + chr4_4:48822584-48822603 None:intergenic 25.0%
! AAGAATCAAACAATGTATTG+AGG + chr4_4:48822585-48822604 None:intergenic 25.0%
! ATATGAAATAAGTAGAGAGA+TGG + chr4_4:48822787-48822806 None:intergenic 25.0%
!!! ATTTCTGACTTTTTTGGTTA+AGG - chr4_4:48822873-48822892 Msa0685000:intron 25.0%
! AATTTAAGACACCAAAGTAG+AGG + chr4_4:48818902-48818921 None:intergenic 30.0%
! TTTCCCAAAGGTTTCATTTT+TGG - chr4_4:48819008-48819027 Msa0685000:CDS 30.0%
GTACCAAAAATGAAACCTTT+GGG + chr4_4:48819014-48819033 None:intergenic 30.0%
AGTACCAAAAATGAAACCTT+TGG + chr4_4:48819015-48819034 None:intergenic 30.0%
! AAAATTTTACAAAACGCACC+TGG + chr4_4:48819052-48819071 None:intergenic 30.0%
!! TTTATGTATTGGTCTTGTTC+TGG - chr4_4:48819571-48819590 Msa0685000:intron 30.0%
ATGTAGCATACTATATATGG+TGG - chr4_4:48819646-48819665 Msa0685000:CDS 30.0%
CTTACTTTCGTTAGTTAAGT+TGG - chr4_4:48819837-48819856 Msa0685000:intron 30.0%
GGGTTAAAAGTTAACAGAAT+TGG + chr4_4:48819864-48819883 None:intergenic 30.0%
AAAACAAAATAATGAGGCCA+TGG + chr4_4:48819923-48819942 None:intergenic 30.0%
!! CACAACCTACCTTTTAATTA+AGG - chr4_4:48820039-48820058 Msa0685000:CDS 30.0%
TCGAACCTTAATTAAAAGGT+AGG + chr4_4:48820047-48820066 None:intergenic 30.0%
TTAAATATGGATACCTTCCT+CGG + chr4_4:48820077-48820096 None:intergenic 30.0%
AAAATATTGGATGGAAGCAA+TGG - chr4_4:48820279-48820298 Msa0685000:intron 30.0%
GCATCATAAGGTAAAATCTA+TGG + chr4_4:48820524-48820543 None:intergenic 30.0%
!! CAAAATGTAAGAGCATCATA+AGG + chr4_4:48820536-48820555 None:intergenic 30.0%
GTTATACTTGGCAATATTCT+TGG + chr4_4:48820670-48820689 None:intergenic 30.0%
TCACCTAAAGTTTCATCTAT+AGG - chr4_4:48820718-48820737 Msa0685000:intron 30.0%
TCACCTATAGATGAAACTTT+AGG + chr4_4:48820724-48820743 None:intergenic 30.0%
!! AGCTCTAAAAAATATGCAGA+AGG - chr4_4:48820771-48820790 Msa0685000:intron 30.0%
! AATGAAGGTATAAACCAAGA+AGG - chr4_4:48820801-48820820 Msa0685000:intron 30.0%
TATCAAGTAGTTCATTGATC+AGG + chr4_4:48820845-48820864 None:intergenic 30.0%
TGGAATTATTGATATAGGTC+TGG - chr4_4:48820923-48820942 Msa0685000:CDS 30.0%
ATACTTGTCTTGTAAAGCTT+GGG + chr4_4:48820982-48821001 None:intergenic 30.0%
TATACTTGTCTTGTAAAGCT+TGG + chr4_4:48820983-48821002 None:intergenic 30.0%
! CATATGCAGAAATGATTTTC+AGG - chr4_4:48821229-48821248 Msa0685000:intron 30.0%
ACTTTATAGAACCAATCATC+AGG - chr4_4:48821394-48821413 Msa0685000:intron 30.0%
ACAAATACAAGAAAGGCTTA+TGG + chr4_4:48821435-48821454 None:intergenic 30.0%
TAACTCAAATTAATGGCATC+AGG - chr4_4:48821479-48821498 Msa0685000:intron 30.0%
CAAAAACCAGTGACAATTTA+AGG + chr4_4:48821532-48821551 None:intergenic 30.0%
CACAATTGTTCACAAAATCA+TGG - chr4_4:48821634-48821653 Msa0685000:CDS 30.0%
! TATATTGGAAGCATAATTGG+AGG + chr4_4:48821840-48821859 None:intergenic 30.0%
! TGGTATATTGGAAGCATAAT+TGG + chr4_4:48821843-48821862 None:intergenic 30.0%
! CTTGTGTCATTATTTGATTG+TGG + chr4_4:48821863-48821882 None:intergenic 30.0%
TCAGCATACGATTGTATAAA+TGG + chr4_4:48822157-48822176 None:intergenic 30.0%
! TCTAAAGTTAGTTATTGGCT+TGG - chr4_4:48822367-48822386 Msa0685000:intron 30.0%
!! CTAAAGTTAGTTATTGGCTT+GGG - chr4_4:48822368-48822387 Msa0685000:intron 30.0%
! AAGTACAAGGATATTTTGCA+TGG - chr4_4:48822906-48822925 Msa0685000:CDS 30.0%
!! ATATTTTGCATGGTCACTTT+TGG - chr4_4:48822916-48822935 Msa0685000:CDS 30.0%
! CACTTTTGGACAATTTTGAA+TGG - chr4_4:48822930-48822949 Msa0685000:CDS 30.0%
AAAGTTAATTCCAAAACGTC+TGG + chr4_4:48822969-48822988 None:intergenic 30.0%
CAAAGTAGAGGAGATATTGA+TGG + chr4_4:48818890-48818909 None:intergenic 35.0%
CATCAATATCTCCTCTACTT+TGG - chr4_4:48818888-48818907 Msa0685000:CDS 35.0%
TAGCTCCTTTGTTCCAATAA+TGG - chr4_4:48818929-48818948 Msa0685000:CDS 35.0%
TACTACGGTTTAGAGAAGTA+CGG + chr4_4:48818986-48819005 None:intergenic 35.0%
! AACCTTTGGGAAAACTACTA+CGG + chr4_4:48819001-48819020 None:intergenic 35.0%
! TGGAAGAGGACAAAGTATTT+GGG - chr4_4:48819367-48819386 Msa0685000:intron 35.0%
AAATATGCTCATGAACATCC+AGG - chr4_4:48819392-48819411 Msa0685000:intron 35.0%
! GCACTTCTTGTGCTAATTTT+AGG - chr4_4:48819731-48819750 Msa0685000:intron 35.0%
GATGTGTCGGACATTAAATA+TGG + chr4_4:48820090-48820109 None:intergenic 35.0%
GTGGGACCAAAAAAAGTTAA+AGG - chr4_4:48820170-48820189 Msa0685000:CDS 35.0%
AATTACTTTCAGTAGCCACA+TGG + chr4_4:48820233-48820252 None:intergenic 35.0%
ATGTGGCTACTGAAAGTAAT+TGG - chr4_4:48820232-48820251 Msa0685000:intron 35.0%
GCAGAAGGAAAAGTAAATGA+AGG - chr4_4:48820786-48820805 Msa0685000:intron 35.0%
TACTTGTCTTGTAAAGCTTG+GGG + chr4_4:48820981-48821000 None:intergenic 35.0%
GCTTTACAAGACAAGTATAG+CGG - chr4_4:48820984-48821003 Msa0685000:intron 35.0%
AATCCATATATCCTCATGTG+GGG + chr4_4:48821177-48821196 None:intergenic 35.0%
GAATCCATATATCCTCATGT+GGG + chr4_4:48821178-48821197 None:intergenic 35.0%
TGAATCCATATATCCTCATG+TGG + chr4_4:48821179-48821198 None:intergenic 35.0%
AAGAAAGGCTTATGGTTCTT+CGG + chr4_4:48821427-48821446 None:intergenic 35.0%
GCTTCTCAAAATGGTTCAAT+TGG - chr4_4:48821501-48821520 Msa0685000:intron 35.0%
!! TCTAACGTGTCATTTGAGAA+CGG + chr4_4:48821555-48821574 None:intergenic 35.0%
CTCTTGATTTCATGTTTGGA+TGG - chr4_4:48821595-48821614 Msa0685000:intron 35.0%
! GAGCAATCTAAGATGCTTAT+TGG - chr4_4:48821782-48821801 Msa0685000:intron 35.0%
TGAGTATCCTTGAAATATCC+AGG + chr4_4:48821890-48821909 None:intergenic 35.0%
CTTGGATAAACAAATAGCCA+AGG + chr4_4:48822209-48822228 None:intergenic 35.0%
TGGCTATTTGTTTATCCAAG+AGG - chr4_4:48822209-48822228 Msa0685000:intron 35.0%
GGCTATTTGTTTATCCAAGA+GGG - chr4_4:48822210-48822229 Msa0685000:intron 35.0%
!!! TGACTTTTTTGGTTAAGGCA+TGG - chr4_4:48822878-48822897 Msa0685000:intron 35.0%
TGCTGCCATTATTGGAACAA+AGG + chr4_4:48818937-48818956 None:intergenic 40.0%
GTAACAGATGCTGCCATTAT+TGG + chr4_4:48818945-48818964 None:intergenic 40.0%
TGTTACTCCTACAACACTTG+TGG - chr4_4:48818959-48818978 Msa0685000:CDS 40.0%
! AACCGTAGTAGTTTTCCCAA+AGG - chr4_4:48818996-48819015 Msa0685000:CDS 40.0%
TACTTAGGGATGGAGTGATA+TGG - chr4_4:48819251-48819270 Msa0685000:CDS 40.0%
!! TATGAAGGTGCTGCAAATGA+AGG - chr4_4:48819344-48819363 Msa0685000:intron 40.0%
! GTGGAAGAGGACAAAGTATT+TGG - chr4_4:48819366-48819385 Msa0685000:intron 40.0%
!! GTCTTGTTCTGGAAGTTAGT+AGG - chr4_4:48819582-48819601 Msa0685000:intron 40.0%
!! TTGTTCTGGAAGTTAGTAGG+AGG - chr4_4:48819585-48819604 Msa0685000:intron 40.0%
!! ACTATATATGGTGGTGTTGC+AGG - chr4_4:48819655-48819674 Msa0685000:CDS 40.0%
TGTGCTCATCTTAGACATTG+GGG + chr4_4:48819884-48819903 None:intergenic 40.0%
GTGTGCTCATCTTAGACATT+GGG + chr4_4:48819885-48819904 None:intergenic 40.0%
TGTGTGCTCATCTTAGACAT+TGG + chr4_4:48819886-48819905 None:intergenic 40.0%
AGATGAGCACACATTGTATG+TGG - chr4_4:48819893-48819912 Msa0685000:intron 40.0%
ACATTGTATGTGGTTCACCA+TGG - chr4_4:48819903-48819922 Msa0685000:intron 40.0%
TAAGGTTCGAATTACAACCG+AGG - chr4_4:48820057-48820076 Msa0685000:CDS 40.0%
! AATTTGTTTGGGTTTCTCGC+AGG + chr4_4:48820141-48820160 None:intergenic 40.0%
CATACAGATTCTCCATCTCT+TGG - chr4_4:48820644-48820663 Msa0685000:CDS 40.0%
CAATATTCTTGGCCAAGAGA+TGG + chr4_4:48820659-48820678 None:intergenic 40.0%
! ATTTGTGACCCTTTTCCACT+GGG - chr4_4:48820950-48820969 Msa0685000:CDS 40.0%
GACGTCATAAAGCTCTATCA+TGG + chr4_4:48821137-48821156 None:intergenic 40.0%
! GCTTTATGACGTCGATTGAA+AGG - chr4_4:48821144-48821163 Msa0685000:intron 40.0%
GAATTCCTTGAAGCATAGCT+CGG + chr4_4:48821261-48821280 None:intergenic 40.0%
GAGCTATGCTTCAAGGAATT+CGG - chr4_4:48821260-48821279 Msa0685000:CDS 40.0%
ACACTCTTACAGTTTGGGTA+CGG - chr4_4:48821319-48821338 Msa0685000:intron 40.0%
GCATCACCTTAAATTGTCAC+TGG - chr4_4:48821523-48821542 Msa0685000:intron 40.0%
CGTGCTCTTGATTTCATGTT+TGG - chr4_4:48821591-48821610 Msa0685000:intron 40.0%
AAGCATCTTAGATTGCTCTC+TGG + chr4_4:48821780-48821799 None:intergenic 40.0%
AATAATGACACAAGCACACC+TGG - chr4_4:48821869-48821888 Msa0685000:intron 40.0%
AAGCACACCTGGATATTTCA+AGG - chr4_4:48821880-48821899 Msa0685000:intron 40.0%
AATAGAGTCTTACTCACCCT+TGG + chr4_4:48822079-48822098 None:intergenic 40.0%
GAGAAACGACACTTATGTAC+AGG - chr4_4:48822175-48822194 Msa0685000:intron 40.0%
!! TTAGTTATTGGCTTGGGATC+TGG - chr4_4:48822374-48822393 Msa0685000:intron 40.0%
!! TAGTTATTGGCTTGGGATCT+GGG - chr4_4:48822375-48822394 Msa0685000:intron 40.0%
! ATTCTCACCGGTGTCAATTT+AGG - chr4_4:48822428-48822447 Msa0685000:intron 40.0%
!! GACAATTTTGAATGGCTTGC+CGG - chr4_4:48822938-48822957 Msa0685000:CDS 40.0%
ACCAAAAGCTTTCAGCTCAT+TGG - chr4_4:48823017-48823036 Msa0685000:CDS 40.0%
! ACCAATGAGCTGAAAGCTTT+TGG + chr4_4:48823021-48823040 None:intergenic 40.0%
TACAAAACGCACCTGGTAAG+AGG + chr4_4:48819045-48819064 None:intergenic 45.0%
GTTCGAATTACAACCGAGGA+AGG - chr4_4:48820061-48820080 Msa0685000:CDS 45.0%
AACTGTAGTGACACTCCATG+TGG - chr4_4:48820215-48820234 Msa0685000:CDS 45.0%
CAGTGGAAAAGGGTCACAAA+TGG + chr4_4:48820951-48820970 None:intergenic 45.0%
! CATTTGTGACCCTTTTCCAC+TGG - chr4_4:48820949-48820968 Msa0685000:CDS 45.0%
AAGGTAATTGACCCCACATG+AGG - chr4_4:48821163-48821182 Msa0685000:intron 45.0%
TGACCCCACATGAGGATATA+TGG - chr4_4:48821171-48821190 Msa0685000:intron 45.0%
! GTAAGAGTGTGGCTCATTGA+GGG + chr4_4:48821309-48821328 None:intergenic 45.0%
! TGTAAGAGTGTGGCTCATTG+AGG + chr4_4:48821310-48821329 None:intergenic 45.0%
TGAGCCACACTCTTACAGTT+TGG - chr4_4:48821313-48821332 Msa0685000:CDS 45.0%
GAGCCACACTCTTACAGTTT+GGG - chr4_4:48821314-48821333 Msa0685000:intron 45.0%
GTACCCAAACTGTAAGAGTG+TGG + chr4_4:48821320-48821339 None:intergenic 45.0%
TGGCATCAGGCTTCTCAAAA+TGG - chr4_4:48821492-48821511 Msa0685000:intron 45.0%
CACGTTAGATCATCAAGCTG+CGG - chr4_4:48821566-48821585 Msa0685000:intron 45.0%
TTTATGCAGCCACTGACAAC+AGG - chr4_4:48821701-48821720 Msa0685000:intron 45.0%
!! AGGAGAGTATCCATCAAGCA+TGG - chr4_4:48821721-48821740 Msa0685000:intron 45.0%
ACGAAAGACACCATGCTTGA+TGG + chr4_4:48821734-48821753 None:intergenic 45.0%
ATCAAGCATGGTGTCTTTCG+TGG - chr4_4:48821733-48821752 Msa0685000:intron 45.0%
TCAAGCATGGTGTCTTTCGT+GGG - chr4_4:48821734-48821753 Msa0685000:intron 45.0%
GATTGCTCTCTGGTGAACTT+TGG + chr4_4:48821770-48821789 None:intergenic 45.0%
CCTCATAGGAGTTGTGATCA+AGG + chr4_4:48821936-48821955 None:intergenic 45.0%
CCTTGATCACAACTCCTATG+AGG - chr4_4:48821933-48821952 Msa0685000:intron 45.0%
! CTTTTGACAGCTGAGCGTAA+CGG - chr4_4:48822040-48822059 Msa0685000:intron 45.0%
! TTTTGACAGCTGAGCGTAAC+GGG - chr4_4:48822041-48822060 Msa0685000:intron 45.0%
TTACTCACCCTTGGTCCAAT+TGG + chr4_4:48822070-48822089 None:intergenic 45.0%
AGCAGTTCTTGAATCCCTCT+TGG + chr4_4:48822227-48822246 None:intergenic 45.0%
! TCACCGGTGTCAATTTAGGT+GGG - chr4_4:48822432-48822451 Msa0685000:intron 45.0%
TCCAACGCTATCACTTGAAG+AGG - chr4_4:48822684-48822703 Msa0685000:intron 45.0%
! AGGCATGGTGTGAAAGTACA+AGG - chr4_4:48822893-48822912 Msa0685000:CDS 45.0%
TCCAAAACGTCTGGTGTATC+CGG + chr4_4:48822960-48822979 None:intergenic 45.0%
!!! TATGTTTTTAAATTTTTTAA+TGG - chr4_4:48819174-48819193 Msa0685000:CDS 5.0%
!!! TAAATTTAGTTTTAATTTAA+TGG - chr4_4:48821088-48821107 Msa0685000:CDS 5.0%
!!! TAAATAAGATTTTAAATTAT+GGG + chr4_4:48821677-48821696 None:intergenic 5.0%
!!! ATAAATAAGATTTTAAATTA+TGG + chr4_4:48821678-48821697 None:intergenic 5.0%
!!! TAATTTAAAATCTTATTTAT+AGG - chr4_4:48821678-48821697 Msa0685000:intron 5.0%
!!! AAATTTTAATAACTAAATTT+AGG - chr4_4:48822643-48822662 Msa0685000:intron 5.0%
! GTACGGTCCACAAGTGTTGT+AGG + chr4_4:48818969-48818988 None:intergenic 50.0%
TACTGCCTCATCCTCTTACC+AGG - chr4_4:48819031-48819050 Msa0685000:intron 50.0%
!! GAAGGTGCTGCAAATGAAGG+TGG - chr4_4:48819347-48819366 Msa0685000:intron 50.0%
GCTGCAAATGAAGGTGGAAG+AGG - chr4_4:48819353-48819372 Msa0685000:intron 50.0%
GGGAAGATCCCAGTGGAAAA+GGG + chr4_4:48820961-48820980 None:intergenic 50.0%
CTATGCCGAGCTATGCTTCA+AGG - chr4_4:48821253-48821272 Msa0685000:intron 50.0%
TCGGAGACAGAGTGAAGCAT+TGG - chr4_4:48821279-48821298 Msa0685000:CDS 50.0%
TTTGGGTACGGAGCCTTACA+TGG - chr4_4:48821331-48821350 Msa0685000:intron 50.0%
TGGTTGTGTGAGGCCATGTA+AGG + chr4_4:48821347-48821366 None:intergenic 50.0%
! AGCAAGGAGTTGGTTGTGTG+AGG + chr4_4:48821357-48821376 None:intergenic 50.0%
!! TCGGTGTTGCACCTGATGAT+TGG + chr4_4:48821408-48821427 None:intergenic 50.0%
GGATACTCTCCTGTTGTCAG+TGG + chr4_4:48821713-48821732 None:intergenic 50.0%
! GAGCGTAACGGGATACCAAT+TGG - chr4_4:48822052-48822071 Msa0685000:intron 50.0%
ACGGGATACCAATTGGACCA+AGG - chr4_4:48822059-48822078 Msa0685000:intron 50.0%
CGGGATACCAATTGGACCAA+GGG - chr4_4:48822060-48822079 Msa0685000:intron 50.0%
ATGTACAGGCTGCTTCACCT+TGG - chr4_4:48822189-48822208 Msa0685000:intron 50.0%
GTCTCAGGTTCGATTCTCAC+CGG - chr4_4:48822416-48822435 Msa0685000:intron 50.0%
! CTCACCGGTGTCAATTTAGG+TGG - chr4_4:48822431-48822450 Msa0685000:intron 50.0%
! GCCTCTTCAAGTGATAGCGT+TGG + chr4_4:48822688-48822707 None:intergenic 50.0%
!! ATCACTTGAAGAGGCGCTCA+TGG - chr4_4:48822693-48822712 Msa0685000:intron 50.0%
ACGCACCTGGTAAGAGGATG+AGG + chr4_4:48819039-48819058 None:intergenic 55.0%
GGGGAAGATCCCAGTGGAAA+AGG + chr4_4:48820962-48820981 None:intergenic 55.0%
AAGCTTGGGGAAGATCCCAG+TGG + chr4_4:48820968-48820987 None:intergenic 55.0%
CAGCTGCATGAGCAAGGAGT+TGG + chr4_4:48821367-48821386 None:intergenic 55.0%
TGACAGCAGCTGCATGAGCA+AGG + chr4_4:48821373-48821392 None:intergenic 55.0%
GAATCGAACCTGAGACCTCG+AGG + chr4_4:48822412-48822431 None:intergenic 55.0%
CCGCCCACCTAAATTGACAC+CGG + chr4_4:48822438-48822457 None:intergenic 55.0%
! CCGGTGTCAATTTAGGTGGG+CGG - chr4_4:48822435-48822454 Msa0685000:intron 55.0%
! CGGTGTCAATTTAGGTGGGC+GGG - chr4_4:48822436-48822455 Msa0685000:intron 55.0%
! GCCGGATACACCAGACGTTT+TGG - chr4_4:48822956-48822975 Msa0685000:CDS 55.0%
TCGAACCTGAGACCTCGAGG+AGG + chr4_4:48822409-48822428 None:intergenic 60.0%
! TGGGAGTGTGCTCCTCCTCG+AGG - chr4_4:48822394-48822413 Msa0685000:intron 65.0%
GTGCTCCTCCTCGAGGTCTC+AGG - chr4_4:48822401-48822420 Msa0685000:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 48818873 48823066 48818873 ID=Msa0685000;Name=Msa0685000
chr4_4 mRNA 48818873 48823066 48818873 ID=Msa0685000-mRNA-1;Parent=Msa0685000;Name=Msa0685000-mRNA-1;_AED=0.33;_eAED=0.34;_QI=0|0.25|0.15|1|1|1|13|0|494
chr4_4 exon 48822887 48823066 48822887 ID=Msa0685000-mRNA-1:exon:12144;Parent=Msa0685000-mRNA-1
chr4_4 exon 48822526 48822595 48822526 ID=Msa0685000-mRNA-1:exon:12143;Parent=Msa0685000-mRNA-1
chr4_4 exon 48821604 48821662 48821604 ID=Msa0685000-mRNA-1:exon:12142;Parent=Msa0685000-mRNA-1
chr4_4 exon 48821257 48821332 48821257 ID=Msa0685000-mRNA-1:exon:12141;Parent=Msa0685000-mRNA-1
chr4_4 exon 48821072 48821149 48821072 ID=Msa0685000-mRNA-1:exon:12140;Parent=Msa0685000-mRNA-1
chr4_4 exon 48820912 48820999 48820912 ID=Msa0685000-mRNA-1:exon:12139;Parent=Msa0685000-mRNA-1
chr4_4 exon 48820524 48820701 48820524 ID=Msa0685000-mRNA-1:exon:12138;Parent=Msa0685000-mRNA-1
chr4_4 exon 48820323 48820438 48820323 ID=Msa0685000-mRNA-1:exon:12137;Parent=Msa0685000-mRNA-1
chr4_4 exon 48820016 48820239 48820016 ID=Msa0685000-mRNA-1:exon:12136;Parent=Msa0685000-mRNA-1
chr4_4 exon 48819858 48819889 48819858 ID=Msa0685000-mRNA-1:exon:12135;Parent=Msa0685000-mRNA-1
chr4_4 exon 48819640 48819742 48819640 ID=Msa0685000-mRNA-1:exon:12134;Parent=Msa0685000-mRNA-1
chr4_4 exon 48819166 48819274 48819166 ID=Msa0685000-mRNA-1:exon:12133;Parent=Msa0685000-mRNA-1
chr4_4 exon 48818873 48819044 48818873 ID=Msa0685000-mRNA-1:exon:12132;Parent=Msa0685000-mRNA-1
chr4_4 CDS 48822887 48823066 48822887 ID=Msa0685000-mRNA-1:cds;Parent=Msa0685000-mRNA-1
chr4_4 CDS 48822526 48822595 48822526 ID=Msa0685000-mRNA-1:cds;Parent=Msa0685000-mRNA-1
chr4_4 CDS 48821604 48821662 48821604 ID=Msa0685000-mRNA-1:cds;Parent=Msa0685000-mRNA-1
chr4_4 CDS 48821257 48821332 48821257 ID=Msa0685000-mRNA-1:cds;Parent=Msa0685000-mRNA-1
chr4_4 CDS 48821072 48821149 48821072 ID=Msa0685000-mRNA-1:cds;Parent=Msa0685000-mRNA-1
chr4_4 CDS 48820912 48820999 48820912 ID=Msa0685000-mRNA-1:cds;Parent=Msa0685000-mRNA-1
chr4_4 CDS 48820524 48820701 48820524 ID=Msa0685000-mRNA-1:cds;Parent=Msa0685000-mRNA-1
chr4_4 CDS 48820323 48820438 48820323 ID=Msa0685000-mRNA-1:cds;Parent=Msa0685000-mRNA-1
chr4_4 CDS 48820016 48820239 48820016 ID=Msa0685000-mRNA-1:cds;Parent=Msa0685000-mRNA-1
chr4_4 CDS 48819858 48819889 48819858 ID=Msa0685000-mRNA-1:cds;Parent=Msa0685000-mRNA-1
chr4_4 CDS 48819640 48819742 48819640 ID=Msa0685000-mRNA-1:cds;Parent=Msa0685000-mRNA-1
chr4_4 CDS 48819166 48819274 48819166 ID=Msa0685000-mRNA-1:cds;Parent=Msa0685000-mRNA-1
chr4_4 CDS 48818873 48819044 48818873 ID=Msa0685000-mRNA-1:cds;Parent=Msa0685000-mRNA-1
Gene Sequence

>Msa0685000

ATGGCATTCAATGGCCATCAATATCTCCTCTACTTTGGTGTCTTAAATTTTATCATTAGCTCCTTTGTTCCAATAATGGCAGCATCTGTTACTCCTACAACACTTGTGGACCGTACTTCTCTAAACCGTAGTAGTTTTCCCAAAGGTTTCATTTTTGGTACTGCCTCATCCTCTTACCAGTATGAAGGTGCTGCAAATGAAGGTGGAAGAGGACAAAGTATTTGGGATAAATATGCTCATGAACATCCAGAAAAAATATTGGATGGAAGCAATGGAGATGTAGCTGTTGATCAATATCATCGTTATAAGGAAGATGTTGCAATCATGAAGTATATGAATACAGATGCATACAGATTCTCCATCTCTTGGCCAAGAATATTGCCAAAAGGAAAAGTAAATGAAGGTATAAACCAAGAAGGAATCAAATATTACAACAACCTGATCAATGAACTACTTGATAATGGTCTGGTACCATTTGTGACCCTTTTCCACTGGGATCTTCCCCAAGCTTTACAAGACAAGTATAGCGGCTTCTTAAGCCCTAATATTATAAATGATTTTCAGGACTATGCCGAGCTATGCTTCAAGGAATTCGGAGACAGAGTGAAGCATTGGATTACCCTCAATGAGCCACACTCTTACAGTTTGGGTACGGAGCCTTACATGGCCTCACACAACCAACTCCTTGCTCATGCAGCTGCTGTCAAACTTTATAGAACCAATCATCAGGCTTCTCAAAATGGTTCAATTGGCATCACCTTAAATTGTCACTGGTTTTTGCCGTTCTCAAATGACACGTTAGATCATCAAGCTGCGGAACGTGCTCTTGATTTCATGTTTGGATGGTTTATGCAGCCACTGACAACAGGAGAGTATCCATCAAGCATGGTGTCTTTCGTGGGAAATCGATTACCAAAGTTCACCAGAGAGCAATCTAAGATGCTTATTGGTTCATTTGATTTTATTGGAATAAATTACTACACCTCCAATTATGCTTCCAATATACCACAATCAAATAATGACACAAGCACACCTGGATATTTCAAGGATACTCATGTCAATTTAACAACTGAGCGTAACGGGATACCAATTGGACCAAGGGCTGCTTCACCTTGGCTATTTGTTTATCCAAGAGGGATTCAAGAACTGCTGTTGTACACAAAGACAAAGTATAACAATCCTTTGATTTACATAACAGAAAATGGTATGGACGAGTTAAATGATCCAACGCTATCACTTGAAGAGGCGCTCATGGATACTTACAGAATCAATTACTTTTATCGACATCTTTATTATATTTCATCTGCAATCAAGCATGGTGTGAAAGTACAAGGATATTTTGCATGGTCACTTTTGGACAATTTTGAATGGCTTGCCGGATACACCAGACGTTTTGGAATTAACTTTGTAGATTACAAAGATAATCTTAAAAGACACCAAAAGCTTTCAGCTCATTGGTTTAGAAATTTTCTTAAAAAGCAGTAG
Protein sequence

>Msa0685000

MAFNGHQYLLYFGVLNFIISSFVPIMAASVTPTTLVDRTSLNRSSFPKGFIFGTASSSYQYEGAANEGGRGQSIWDKYAHEHPEKILDGSNGDVAVDQYHRYKEDVAIMKYMNTDAYRFSISWPRILPKGKVNEGINQEGIKYYNNLINELLDNGLVPFVTLFHWDLPQALQDKYSGFLSPNIINDFQDYAELCFKEFGDRVKHWITLNEPHSYSLGTEPYMASHNQLLAHAAAVKLYRTNHQASQNGSIGITLNCHWFLPFSNDTLDHQAAERALDFMFGWFMQPLTTGEYPSSMVSFVGNRLPKFTREQSKMLIGSFDFIGINYYTSNYASNIPQSNNDTSTPGYFKDTHVNLTTERNGIPIGPRAASPWLFVYPRGIQELLLYTKTKYNNPLIYITENGMDELNDPTLSLEEALMDTYRINYFYRHLYYISSAIKHGVKVQGYFAWSLLDNFEWLAGYTRRFGINFVDYKDNLKRHQKLSAHWFRNFLKKQ*